#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
intact:EBI-26876600	uniprotkb:P0DTD1-PRO_0000449628	-	intact:EBI-25475880	psi-mi:ds_h4_rna(display_short)|psi-mi:EBI-26876600(display_long)	psi-mi:p0dtd1-pro_0000449628(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:"MI:0920"(ribonuclease assay)	Saramago et al. (2021)	pubmed:33705595|imex:IM-28812	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	psi-mi:"MI:0902"(rna cleavage)	psi-mi:"MI:0469"(IntAct)	intact:EBI-26876561|imex:IM-28812-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0840"(stimulator)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:2359"(ds rna)	psi-mi:"MI:0326"(protein)	-	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	go:"GO:0004527"(exonuclease activity)	-	chain-seq-start:4254|chain-seq-end:4392	figure legend:1C, 1E, 2, 4D, 5C|comment:Co-purifying nsp14  and nsp10  proteins  substantially increases  nsp14 ExoNase activity.|agonist:MnCl2|agonist:Mgcl2|comment:Addition of  ZnCl2 and CoCl2 caused a slight increase in ExoN activity.|antagonist:"EDTA (CHEBI:42191 )"|comment:"Mutations of nsp10 (with the exception of S72A) reduced ExoNase activity to that of nsp14 alone. The S72A mutation further decreased activity."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/03/12	2021/03/15	-	rogid:L4eD+20oc1IdNFJ9By3H2uun6L02697049	-	false	32p radiolabel:1-1	mutation decreasing interaction rate:80-80|mutation decreasing interaction rate:19-19|mutation decreasing interaction rate:69-69|mutation decreasing interaction rate:96-96|his tag:n-n|mutation decreasing interaction rate:72-72	-	-	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)
intact:EBI-26876600	uniprotkb:P0DTD1-PRO_0000449631	-	intact:EBI-25475920	psi-mi:ds_h4_rna(display_short)|psi-mi:EBI-26876600(display_long)	psi-mi:p0dtd1-pro_0000449631(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:"MI:0920"(ribonuclease assay)	Saramago et al. (2021)	pubmed:33705595|imex:IM-28812	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	psi-mi:"MI:0902"(rna cleavage)	psi-mi:"MI:0469"(IntAct)	intact:EBI-26876561|imex:IM-28812-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:2359"(ds rna)	psi-mi:"MI:0326"(protein)	-	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	go:"GO:0004527"(exonuclease activity)	-	chain-seq-start:5926|chain-seq-end:6452	figure legend:1C, 1E, 2, 4D, 5C|comment:Co-purifying nsp14  and nsp10  proteins  substantially increases  nsp14 ExoNase activity.|agonist:MnCl2|agonist:Mgcl2|comment:Addition of  ZnCl2 and CoCl2 caused a slight increase in ExoN activity.|antagonist:"EDTA (CHEBI:42191 )"|comment:"Mutations of nsp10 (with the exception of S72A) reduced ExoNase activity to that of nsp14 alone. The S72A mutation further decreased activity."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/03/12	2021/03/15	-	rogid:LpGB5BQU5uhWK/60422jkiKHRL82697049	-	false	32p radiolabel:1-1	his tag:n-n	-	-	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)
intact:EBI-26876600	uniprotkb:P0DTD1-PRO_0000449631	-	intact:EBI-25475920	psi-mi:ds_h4_rna(display_short)|psi-mi:EBI-26876600(display_long)	psi-mi:p0dtd1-pro_0000449631(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:"MI:0920"(ribonuclease assay)	Saramago et al. (2021)	pubmed:33705595|imex:IM-28812	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	psi-mi:"MI:0902"(rna cleavage)	psi-mi:"MI:0469"(IntAct)	intact:EBI-26876577|imex:IM-28812-3	-	-	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:2359"(ds rna)	psi-mi:"MI:0326"(protein)	-	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	go:"GO:0004527"(exonuclease activity)	-	chain-seq-start:5926|chain-seq-end:6452	figure legend:1C, 1E, 4D, 5C|comment:nsp14 alone exhibits exoribonucleolytic activity; however low activity was seen.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/03/12	2021/03/15	-	rogid:LpGB5BQU5uhWK/60422jkiKHRL82697049	-	false	32p radiolabel:1-1	his tag:n-n	-	-	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)
intact:EBI-26876600	uniprotkb:P0DTD1-PRO_0000449628	-	intact:EBI-25475880	psi-mi:ds_h4_rna(display_short)|psi-mi:EBI-26876600(display_long)	psi-mi:p0dtd1-pro_0000449628(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:"MI:0920"(ribonuclease assay)	Saramago et al. (2021)	pubmed:33705595|imex:IM-28812	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	psi-mi:"MI:0902"(rna cleavage)	psi-mi:"MI:0469"(IntAct)	intact:EBI-26876772|imex:IM-28812-9	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0840"(stimulator)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:2359"(ds rna)	psi-mi:"MI:0326"(protein)	-	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	go:"GO:0004527"(exonuclease activity)	-	chain-seq-start:4254|chain-seq-end:4392	figure legend:5C|comment:Co-purifying nsp14  and nsp10  proteins  substantially increases  nsp14 ExoNase activity.|agonist:MnCl2|agonist:Mgcl2|comment:Addition of  ZnCl2 and CoCl2 caused a slight increase in ExoN activity.|antagonist:"EDTA (CHEBI:42191 )"|comment:"Mutations of nsp10 (with the exception of S72A) reduced ExoNase activity to that of nsp14 alone. The S72A mutation further decreased activity."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/03/12	2021/03/15	-	rogid:L4eD+20oc1IdNFJ9By3H2uun6L02697049	-	false	32p radiolabel:1-1	his tag:n-n	-	-	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)
intact:EBI-26876600	uniprotkb:P0DTD1-PRO_0000449631	-	intact:EBI-25475920	psi-mi:ds_h4_rna(display_short)|psi-mi:EBI-26876600(display_long)	psi-mi:p0dtd1-pro_0000449631(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:"MI:0920"(ribonuclease assay)	Saramago et al. (2021)	pubmed:33705595|imex:IM-28812	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	psi-mi:"MI:0902"(rna cleavage)	psi-mi:"MI:0469"(IntAct)	intact:EBI-26876772|imex:IM-28812-9	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:2359"(ds rna)	psi-mi:"MI:0326"(protein)	-	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	go:"GO:0004527"(exonuclease activity)	-	chain-seq-start:5926|chain-seq-end:6452	figure legend:5C|comment:Co-purifying nsp14  and nsp10  proteins  substantially increases  nsp14 ExoNase activity.|agonist:MnCl2|agonist:Mgcl2|comment:Addition of  ZnCl2 and CoCl2 caused a slight increase in ExoN activity.|antagonist:"EDTA (CHEBI:42191 )"|comment:"Mutations of nsp10 (with the exception of S72A) reduced ExoNase activity to that of nsp14 alone. The S72A mutation further decreased activity."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/03/12	2021/03/15	-	rogid:LpGB5BQU5uhWK/60422jkiKHRL82697049	-	false	32p radiolabel:1-1	mutation decreasing interaction rate:90-90|his tag:n-n|mutation decreasing interaction rate:92-92|mutation decreasing interaction rate:243-243|mutation decreasing interaction rate:273-273	-	-	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)
uniprotkb:P0DTD1-PRO_0000449628	uniprotkb:P0DTD1-PRO_0000449631	intact:EBI-25475880	intact:EBI-25475920	psi-mi:p0dtd1-pro_0000449628(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:p0dtd1-pro_0000449631(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:"MI:0107"(surface plasmon resonance)	Saramago et al. (2021)	pubmed:33705595|imex:IM-28812	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-26876657|imex:IM-28812-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	-	chain-seq-start:4254|chain-seq-end:4392	chain-seq-start:5926|chain-seq-end:6452	figure legend:1D, 4C|comment:As both proteins are unstable, it was not   possible to obtain a good fit of the data in order to determine the binding kinetics and dissociation constants.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/03/12	2021/03/15	rogid:L4eD+20oc1IdNFJ9By3H2uun6L02697049	rogid:LpGB5BQU5uhWK/60422jkiKHRL82697049	rigid:31XhqL6F4tfEjhLqU/s/PXVionw	false	his tag:n-n|mutation decreasing interaction strength:19-19|mutation decreasing interaction strength:72-72|mutation disrupting interaction strength:69-69|mutation disrupting interaction strength:80-80|mutation disrupting interaction strength:96-96	his tag:n-n	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P0DTD1-PRO_0000449628	uniprotkb:P0DTD1-PRO_0000449631	intact:EBI-25475880	intact:EBI-25475920	psi-mi:p0dtd1-pro_0000449628(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:p0dtd1-pro_0000449631(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:"MI:0071"(molecular sieving)	Saramago et al. (2021)	pubmed:33705595|imex:IM-28812	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-26876687|imex:IM-28812-7	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	-	chain-seq-start:4254|chain-seq-end:4392	chain-seq-start:5926|chain-seq-end:6452	figure legend:1E|comment:When co-purified, there are several peaks that can not be seen when the proteins are purified alone.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2021/03/12	2021/04/04	rogid:L4eD+20oc1IdNFJ9By3H2uun6L02697049	rogid:LpGB5BQU5uhWK/60422jkiKHRL82697049	rigid:31XhqL6F4tfEjhLqU/s/PXVionw	false	his tag:n-n	his tag:n-n	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P0DTD1-PRO_0000449631	intact:EBI-26876841	intact:EBI-25475920	-	psi-mi:p0dtd1-pro_0000449631(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:30mer_gppp-rna(display_short)|psi-mi:EBI-26876841(display_long)	psi-mi:"MI:0515"(methyltransferase assay)	Saramago et al. (2021)	pubmed:33705595|imex:IM-28812	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	taxid:-1(in vitro)|taxid:-1(In vitro)	psi-mi:"MI:0213"(methylation reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-26876831|imex:IM-28812-11	-	-	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0320"(ribonucleic acid)	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	-	go:"GO:0001510"(RNA methylation)	chain-seq-start:5926|chain-seq-end:6452	-	figure legend:6|comment:nsp14  MTase  activity    seems  to  be independent of the presence of nsp10 cofactor.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/03/12	2021/03/15	rogid:LpGB5BQU5uhWK/60422jkiKHRL82697049	-	-	false	his tag:n-n|mutation with no effect:90-90|mutation with no effect:92-92|mutation with no effect:243-243|mutation with no effect:273-273|mutation disrupting interaction rate:331-331	32p radiolabel:1-1	-	-	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)