#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q8BIZ1	uniprotkb:P30416	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-492746|uniprotkb:Q3TVC9|ensembl:ENSMUSP00000032508	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:fkbp4_mouse(display_long)|uniprotkb:FK506-binding protein 4(gene name synonym)|uniprotkb:HSP-binding immunophilin(gene name synonym)|uniprotkb:Rotamase(gene name synonym)|uniprotkb:52 kDa FK506-binding protein(gene name synonym)|uniprotkb:FKBP59(gene name synonym)|uniprotkb:Immunophilin FKBP52(gene name synonym)|uniprotkb:Fkbp4(gene name)|psi-mi:Fkbp4(display_short)|uniprotkb:Fkpb52(gene name synonym)|uniprotkb:59 kDa immunophilin(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000030357(gene)|refseq:NP_034349.1|go:"GO:0044295"(axonal growth cone)|go:"GO:0046661"(male sex differentiation)|go:"GO:0048156"(tau protein binding)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0048608"(reproductive structure development)|go:"GO:0051219"(phosphoprotein binding)|go:"GO:0061077"(chaperone-mediated protein folding)|interpro:IPR001179(Peptidyl-prolyl cis-trans isomerase, FKBP-type)|interpro:IPR001440(Tetratricopeptide TPR-1)|interpro:IPR011990(Tetratricopeptide-like helical)|interpro:IPR013105(Tetratricopeptide TPR2)|interpro:IPR019734|interpro:IPR031212|mint:P30416|reactome:R-MMU-3371497|reactome:R-MMU-3371568|reactome:R-MMU-8939211|reactome:R-MMU-9018519|ensembl:ENSMUST00000032508(transcript)|go:"GO:0000413"(protein peptidyl-prolyl isomerization)|go:"GO:0003755"(peptidyl-prolyl cis-trans isomerase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005525"(GTP binding)|go:"GO:0005528"(FK506 binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005829"(cytosol)|go:"GO:0005874"(microtubule)|go:"GO:0006463"(steroid hormone receptor complex assembly)|go:"GO:0006825"(copper ion transport)|go:"GO:0007566"(embryo implantation)|go:"GO:0010977"(negative regulation of neuron projection development)|go:"GO:0030521"(androgen receptor signaling pathway)|go:"GO:0030850"(prostate gland development)|go:"GO:0031072"(heat shock protein binding)|go:"GO:0031111"(negative regulation of microtubule polymerization or depolymerization)|go:"GO:0031115"(negative regulation of microtubule polymerization)|go:"GO:0031503"(protein-containing complex localization)|go:"GO:0032767"(copper-dependent protein binding)|go:"GO:0032991"(protein-containing complex)|go:"GO:0035259"(glucocorticoid receptor binding)|go:"GO:0043005"(neuron projection)|go:"GO:0043025"(neuronal cell body)	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:EPHHukqiCLO3TuZcToruUwMFbtU10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P35282	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-1993555|uniprotkb:Q7TPN7|ensembl:ENSMUSP00000020343	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:rab21_mouse(display_long)|uniprotkb:Rab21(gene name)|psi-mi:Rab21(display_short)|uniprotkb:Rab-12(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_077774.1|ensembl:ENSMUSG00000020132(gene)|ensembl:ENSMUST00000020343(transcript)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005768"(endosome)|go:"GO:0005769"(early endosome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0008089"(anterograde axonal transport)|go:"GO:0009898"(cytoplasmic side of plasma membrane)|go:"GO:0012505"(endomembrane system)|go:"GO:0012506"(vesicle membrane)|go:"GO:0017157"(regulation of exocytosis)|go:"GO:0019003"(GDP binding)|go:"GO:0030516"(regulation of axon extension)|go:"GO:0032154"(cleavage furrow)|go:"GO:0032482"(Rab protein signal transduction)|go:"GO:0032580"(Golgi cisterna membrane)|go:"GO:0043005"(neuron projection)|go:"GO:0045202"(synapse)|go:"GO:0048260"(positive regulation of receptor-mediated endocytosis)|go:"GO:0050775"(positive regulation of dendrite morphogenesis)|go:"GO:0050821"(protein stabilization)|go:"GO:0098559"(cytoplasmic side of early endosome membrane)|go:"GO:1904115"(axon cytoplasm)|go:"GO:2000643"(positive regulation of early endosome to late endosome transport)|interpro:IPR001806(Ras GTPase)|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR020849|interpro:IPR027417|interpro:IPR041833|mint:P35282|reactome:R-MMU-8873719|reactome:R-MMU-8876198	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:LutxdtFI7bIYCtGadJaQ5DdohYY10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9D7X3	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-8320546|intact:MINT-4093702|ensembl:ENSMUSP00000003612|ensembl:ENSMUSP00000102790	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:dus3_mouse(display_long)|uniprotkb:Dusp3(gene name)|psi-mi:Dusp3(display_short)|uniprotkb:T-DSP11(gene name synonym)|uniprotkb:Vaccinia H1-related phosphatase(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_082483.1|ensembl:ENSMUSG00000003518(gene)|ensembl:ENSMUST00000003612(transcript)|ensembl:ENSMUST00000107172(transcript)|go:"GO:0000188"(obsolete inactivation of MAPK activity)|go:"GO:0001701"(in utero embryonic development)|go:"GO:0001772"(immunological synapse)|go:"GO:0004721"(phosphoprotein phosphatase activity)|go:"GO:0004725"(protein tyrosine phosphatase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006470"(protein dephosphorylation)|go:"GO:0008092"(cytoskeletal protein binding)|go:"GO:0008138"(protein tyrosine/serine/threonine phosphatase activity)|go:"GO:0016311"(dephosphorylation)|go:"GO:0016791"(phosphatase activity)|go:"GO:0019901"(protein kinase binding)|go:"GO:0030336"(negative regulation of cell migration)|go:"GO:0030971"(receptor tyrosine kinase binding)|go:"GO:0033549"(MAP kinase phosphatase activity)|go:"GO:0035335"(peptidyl-tyrosine dephosphorylation)|go:"GO:0042059"(negative regulation of epidermal growth factor receptor signaling pathway)|go:"GO:0043409"(negative regulation of MAPK cascade)|go:"GO:0045931"(positive regulation of mitotic cell cycle)|go:"GO:0046329"(negative regulation of JNK cascade)|go:"GO:0050860"(negative regulation of T cell receptor signaling pathway)|go:"GO:0050868"(negative regulation of T cell activation)|go:"GO:0050922"(negative regulation of chemotaxis)|go:"GO:0051893"(regulation of focal adhesion assembly)|go:"GO:0070373"(negative regulation of ERK1 and ERK2 cascade)|go:"GO:0071364"(cellular response to epidermal growth factor stimulus)|go:"GO:0106306"|go:"GO:0106307"|go:"GO:0120183"(positive regulation of focal adhesion disassembly)|go:"GO:1990782"(protein tyrosine kinase binding)|interpro:IPR000340(Protein-tyrosine phosphatase, dual specificity)|interpro:IPR000387(Protein-tyrosine phosphatase)|interpro:IPR016130(Protein-tyrosine phosphatase, active site)|interpro:IPR020405|interpro:IPR020422|interpro:IPR029021|mint:Q9D7X3|reactome:R-MMU-202670|go:"GO:1990264"(peptidyl-tyrosine dephosphorylation involved in inactivation of protein kinase activity)	-	-	comment:mint|function:This protein shows activity both toward tyrosine-protein phosphate as well as with serine-protein phosphate	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:CPkFDe9SCv4Nq8Dwl5NRteIKckg10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q8C9B9	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-8340028|uniprotkb:A2AJ47|uniprotkb:Q3ZTP5|uniprotkb:A2AJ48|uniprotkb:Q4V9W1|uniprotkb:Q8BMD0|uniprotkb:Q8BRG2|uniprotkb:Q8CHR5|uniprotkb:B2RS46|uniprotkb:Q6ZQD7|uniprotkb:Q3ZTP6|uniprotkb:Q05C59|uniprotkb:Q80V34|uniprotkb:Q9WV00|intact:MINT-4111472|ensembl:ENSMUSP00000084794	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:dido1_mouse(display_long)|uniprotkb:Dido1(gene name)|psi-mi:Dido1(display_short)|uniprotkb:Datf1(gene name synonym)|uniprotkb:Dio1(gene name synonym)|uniprotkb:Death-associated transcription factor 1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_808520.2|refseq:NP_780760.2|refseq:NP_035935.2|refseq:XP_006500671.1|refseq:XP_006500672.1|refseq:XP_006500673.1|refseq:XP_006500674.1|refseq:XP_006500675.1|refseq:XP_006500678.1|refseq:XP_006500679.1|refseq:NP_001278361.1|refseq:NP_001278362.1|ensembl:ENSMUSG00000038914(gene)|ensembl:ENSMUST00000087517(transcript)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005819"(spindle)|go:"GO:0006351"(transcription, DNA-templated)|go:"GO:0046872"(metal ion binding)|go:"GO:0097190"(apoptotic signaling pathway)|interpro:IPR001965(Zinc finger, PHD-type)|interpro:IPR003618(Transcription elongation factor S-II, central region)|interpro:IPR011011(Zinc finger, FYVE/PHD-type)|interpro:IPR012921(Spen paralogue and orthologue C-terminal)|interpro:IPR013083(Zinc finger, RING/FYVE/PHD-type)|interpro:IPR019786|interpro:IPR019787|interpro:IPR033082|interpro:IPR036575|mint:Q8C9B9|rcsb pdb:1WEM	-	-	comment:mint|function:Putative transcription factor, weakly pro-apoptotic when overexpressed	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:dMthBBfD+e96GyEkwCBsH7lsJ/810090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9DB72	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-8317600|intact:MINT-4128116|ensembl:ENSMUSP00000000206	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:btbdh_mouse(display_long)|uniprotkb:Btbd17(gene name)|psi-mi:Btbd17(display_short)|uniprotkb:Galectin-3-binding protein-like(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_082331.1|ensembl:ENSMUSG00000000202(gene)|ensembl:ENSMUST00000000206(transcript)|go:"GO:0005576"(extracellular region)|interpro:IPR000210(BTB/POZ-like)|interpro:IPR011333(BTB/POZ fold)|interpro:IPR011705(BTB/Kelch-associated)|mint:Q9DB72	-	-	comment:mint	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:Nr7MrwB8aSTupWcjW8h8wYwEHuk10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q64518	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-8402639|uniprotkb:Q64517|uniprotkb:Q8VD16|intact:MINT-4088610|uniprotkb:O70625|ensembl:ENSMUSP00000104125	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:at2a3_mouse(display_long)|uniprotkb:Atp2a3(gene name)|psi-mi:Atp2a3(display_short)|uniprotkb:Calcium pump 3(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001156809.1|refseq:NP_058025.2|refseq:NP_001156808.1|ensembl:ENSMUSG00000020788(gene)|ensembl:ENSMUST00000108485(transcript)|go:"GO:0005388"(P-type calcium transporter activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0006874"(cellular calcium ion homeostasis)|go:"GO:0006919"(activation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0008553"(P-type proton-exporting transporter activity)|go:"GO:0008656"(cysteine-type endopeptidase activator activity involved in apoptotic process)|go:"GO:0015085"(calcium ion transmembrane transporter activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016529"(sarcoplasmic reticulum)|go:"GO:0030899"(calcium-dependent ATPase activity)|go:"GO:0031090"(organelle membrane)|go:"GO:0032469"(endoplasmic reticulum calcium ion homeostasis)|go:"GO:0033017"(sarcoplasmic reticulum membrane)|go:"GO:0044325"(transmembrane transporter binding)|go:"GO:0046872"(metal ion binding)|go:"GO:0070059"(intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress)|go:"GO:0070588"(calcium ion transmembrane transport)|go:"GO:1900121"(negative regulation of receptor binding)|go:"GO:1903515"(calcium ion transport from cytosol to endoplasmic reticulum)|interpro:IPR001757(ATPase, P-type, K/Mg/Cd/Cu/Zn/Na/Ca/Na/H-transporter)|interpro:IPR004014(ATPase, P-type cation-transporter, N-terminal)|interpro:IPR005782(Calcium ATPase)|interpro:IPR006068(ATPase, P-type cation-transporter, C-terminal)|interpro:IPR008250(E1-E2 ATPase-associated region)|interpro:IPR018303|interpro:IPR023214|interpro:IPR023298|interpro:IPR023299|interpro:IPR036412|interpro:IPR044492|mint:Q64518|reactome:R-MMU-418359|reactome:R-MMU-5578775|reactome:R-MMU-936837	-	-	comment:mint|function:This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:qjTQv/32Eu7jgDPY2ve+A1f9t+s10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q8BH66	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-8314525|ensembl:ENSMUSP00000021466|uniprotkb:Q8BJH5|intact:MINT-4135323	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:atla1_mouse(display_long)|uniprotkb:Atl1(gene name)|psi-mi:Atl1(display_short)|uniprotkb:Spastic paraplegia 3A homolog(gene name synonym)|uniprotkb:Spg3a(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000021066(gene)|ensembl:ENSMUST00000021466(transcript)|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0007029"(endoplasmic reticulum organization)|go:"GO:0007409"(axonogenesis)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030424"(axon)|go:"GO:0042802"(identical protein binding)|go:"GO:0051260"(protein homooligomerization)|go:"GO:0071782"(endoplasmic reticulum tubular network)|go:"GO:0098826"(endoplasmic reticulum tubular network membrane)|go:"GO:1990809"(endoplasmic reticulum tubular network membrane organization)|interpro:IPR015894(Guanylate-binding protein, N-terminal)|interpro:IPR027417|interpro:IPR030386|interpro:IPR036543|mint:Q8BH66|refseq:NP_848743.1	-	-	function:May play a role for proper Golgi membrane dynamics or vesicle trafficking. Functions as a GTPase|comment:mint	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:0QE83v1xxQYB2vWz6Z5QyXzt0U010090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q02357	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-8319704|uniprotkb:Q78E45|uniprotkb:Q3TZ35|uniprotkb:P97446|uniprotkb:Q61302|uniprotkb:P70440|uniprotkb:Q61303|intact:MINT-255055|uniprotkb:Q3UH42|uniprotkb:Q3UHP2|uniprotkb:P97941|uniprotkb:Q3UYY7	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:ank1_mouse(display_long)|uniprotkb:Ank1(gene name)|psi-mi:Ank1(display_short)|uniprotkb:Ank-1(gene name synonym)|uniprotkb:Erythrocyte ankyrin(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001297365.1|refseq:NP_001297366.1|refseq:NP_001104253.1|interpro:IPR020683|refseq:NP_001264209.1|refseq:NP_112435.2|refseq:NP_001264215.1|refseq:NP_001264210.1|refseq:NP_001264218.1|mint:Q02357|interpro:IPR040745|interpro:IPR036770|interpro:IPR000488(Death)|interpro:IPR000906(ZU5)|interpro:IPR002110(Ankyrin)|interpro:IPR011029(DEATH-like)|go:"GO:0005634"(nucleus)|go:"GO:0005886"(plasma membrane)|go:"GO:0006779"(porphyrin-containing compound biosynthetic process)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0007165"(signal transduction)|go:"GO:0008093"(cytoskeletal anchor activity)|go:"GO:0009898"(cytoplasmic side of plasma membrane)|go:"GO:0010638"(positive regulation of organelle organization)|go:"GO:0014731"(spectrin-associated cytoskeleton)|go:"GO:0016020"(membrane)|go:"GO:0016529"(sarcoplasmic reticulum)|go:"GO:0019899"(enzyme binding)|go:"GO:0019903"(protein phosphatase binding)|go:"GO:0030018"(Z disc)|go:"GO:0030507"(spectrin binding)|go:"GO:0030673"(axolemma)|go:"GO:0030863"(cortical cytoskeleton)|go:"GO:0031430"(M band)|go:"GO:0031672"(A band)|go:"GO:0042383"(sarcolemma)|go:"GO:0043005"(neuron projection)|go:"GO:0044325"(transmembrane transporter binding)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0048821"(erythrocyte development)|go:"GO:0051117"(ATPase binding)|go:"GO:0055072"(iron ion homeostasis)|go:"GO:0072659"(protein localization to plasma membrane)|go:"GO:0098662"(inorganic cation transmembrane transport)|reactome:R-MMU-445095|reactome:R-MMU-447043|reactome:R-MMU-6807878	-	-	comment:mint|function:"Attaches integral membrane proteins to cytoskeletal elements; binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin. Erythrocyte ankyrins also link spectrin (beta chain) to the cytoplasmic domain of the erythrocytes anion exchange protein; they retain most or all of these binding functions. In skeletal muscle, isoform Mu7 together with obscurin may provide a molecular link between the sarcoplasmic reticulum and myofibrils"	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:GprhtwVP5FEWl+UJrqrJoU8RjBw10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q8C103	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-8393653|intact:MINT-4113920	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:q8c103_mouse(display_long)|uniprotkb:Ankhd1(gene name)|psi-mi:Ankhd1(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	interpro:IPR020683|refseq:NP_780584.2|interpro:IPR036770|mint:Q8C103|go:"GO:0005737"(cytoplasm)|go:"GO:0045087"(innate immune response)|interpro:IPR002110(Ankyrin)	-	-	comment:"fragmentation type: fragment"|comment:mint	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:7dxkzG95vtHdfZGWbCUH3ej6zAE10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q6P1B1	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-8318710|uniprotkb:Q91Y37|intact:MINT-4121768|ensembl:ENSMUSP00000157689|ensembl:ENSMUSP00000158390	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:xpp1_mouse(display_long)|uniprotkb:Xpnpep1(gene name)|psi-mi:Xpnpep1(display_short)|uniprotkb:Soluble aminopeptidase P(gene name synonym)|uniprotkb:X-Pro aminopeptidase 1(gene name synonym)|uniprotkb:X-prolyl aminopeptidase 1, soluble(gene name synonym)|uniprotkb:Aminoacylproline aminopeptidase(gene name synonym)|uniprotkb:Cytosolic aminopeptidase P(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:XP_011245461.1|refseq:XP_011245462.1|refseq:NP_573479.3|ensembl:ENSMUSG00000025027(gene)|ensembl:ENSMUST00000236058(transcript)|ensembl:ENSMUST00000237294(transcript)|go:"GO:0004177"(aminopeptidase activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006508"(proteolysis)|go:"GO:0010815"(bradykinin catabolic process)|go:"GO:0030145"(manganese ion binding)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0070006"(metalloaminopeptidase activity)|interpro:IPR000587(Creatinase)|interpro:IPR000994(Peptidase M24, catalytic core)|interpro:IPR001131(Peptidase M24B, X-Pro dipeptidase/aminopeptidase P, conserved site)|interpro:IPR029149|interpro:IPR032416|interpro:IPR033740|interpro:IPR036005|mint:Q6P1B1	-	-	comment:mint	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:tfx7qMu+NWY8/W0M0Kn/qosDT9Y10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9JMA1	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-8321236|uniprotkb:Q9D0L0|intact:MINT-4139172|uniprotkb:Q923F2|uniprotkb:Q543U5|ensembl:ENSMUSP00000089728	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:ubp14_mouse(display_long)|uniprotkb:Usp14(gene name)|psi-mi:Usp14(display_short)|uniprotkb:Ubiquitin thioesterase 14(gene name synonym)|uniprotkb:Ubiquitin-specific-processing protease 14(gene name synonym)|uniprotkb:Deubiquitinating enzyme 14(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_067497.2|ensembl:ENSMUSG00000047879(gene)|ensembl:ENSMUST00000092096(transcript)|go:"GO:0000502"(proteasome complex)|go:"GO:0004843"(thiol-dependent deubiquitinase)|go:"GO:0004866"(endopeptidase inhibitor activity)|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0009986"(cell surface)|go:"GO:0016579"(protein deubiquitination)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0043161"(proteasome-mediated ubiquitin-dependent protein catabolic process)|go:"GO:0045087"(innate immune response)|go:"GO:0045202"(synapse)|go:"GO:0050920"(regulation of chemotaxis)|go:"GO:0061136"(regulation of proteasomal protein catabolic process)|go:"GO:0070628"(proteasome binding)|go:"GO:1903070"(negative regulation of ER-associated ubiquitin-dependent protein catabolic process)|interpro:IPR000626(Ubiquitin)|interpro:IPR001394(Peptidase C19, ubiquitin carboxyl-terminal hydrolase 2)|interpro:IPR018200|interpro:IPR019954|interpro:IPR028889|interpro:IPR029071|interpro:IPR038765|interpro:IPR044635|mint:Q9JMA1|rcsb pdb:1WGG|reactome:R-MMU-5689880|go:"GO:0005886"(plasma membrane)	-	-	comment:mint	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:ACr2couis5eQlWoJb5Qfrg3Lt6Y10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9Z1Z0	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-7597484|uniprotkb:Q3U1C7|uniprotkb:Q3TH58|uniprotkb:Q3UMW6|uniprotkb:Q91WE7|intact:MINT-1864334|uniprotkb:Q99JZ5|uniprotkb:Q3T9L9|ensembl:ENSMUSP00000031355	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:uso1_mouse(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_062363.1|ensembl:ENSMUSG00000029407(gene)|ensembl:ENSMUST00000031355(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|mint:Q9Z1Z0|reactome:R-MMU-204005|reactome:R-MMU-6807878	-	-	comment:mint|function:General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:T+8c4kaecmaiMJ5V2z+G+i5ZeIw10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q8BK08	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-8333477|uniprotkb:Q5NCT6|intact:MINT-4111033|ensembl:ENSMUSP00000059494	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:tmm11_mouse(display_long)|uniprotkb:Tmem11(gene name)|psi-mi:Tmem11(display_short)|uniprotkb:Protein PM1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_775655.1|ensembl:ENSMUSG00000043284(gene)|ensembl:ENSMUST00000062677(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0007005"(mitochondrion organization)|go:"GO:0007007"(inner mitochondrial membrane organization)|go:"GO:0031305"(integral component of mitochondrial inner membrane)|interpro:IPR026120|mint:Q8BK08	-	-	comment:mint	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:mmQrr+TaIqRGfPz6B7JxIJn3ES010090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P70297	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-7389748|intact:MINT-96905|ensembl:ENSMUSP00000100025	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:stam1_mouse(display_long)|uniprotkb:Stam(gene name)|psi-mi:Stam(display_short)|uniprotkb:Stam1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_035614.1|ensembl:ENSMUSG00000026718(gene)|ensembl:ENSMUST00000102960(transcript)|go:"GO:0031901"(early endosome membrane)|go:"GO:0033565"(ESCRT-0 complex)|go:"GO:0035091"(phosphatidylinositol binding)|go:"GO:0043130"(ubiquitin binding)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0043328"(protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway)|go:"GO:0044389"(ubiquitin-like protein ligase binding)|go:"GO:1903543"(positive regulation of exosomal secretion)|go:"GO:1903551"(regulation of extracellular exosome assembly)|interpro:IPR001452(Src homology-3)|interpro:IPR002014(VHS)|interpro:IPR003903(Ubiquitin interacting motif)|interpro:IPR008942(ENTH/VHS)|interpro:IPR035657|interpro:IPR036028|mint:P70297|reactome:R-MMU-182971|reactome:R-MMU-5689901|reactome:R-MMU-6807004|reactome:R-MMU-8856825|reactome:R-MMU-8856828|reactome:R-MMU-9013420|reactome:R-MMU-917729	-	-	comment:mint|function:Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and c-myc induction. May also play a role in endocytic trafficking as well as in T-cell development	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:PPoDLENlcg2H6/QBtyxf5au2Vow10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P26638	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-8318893|intact:MINT-4136532|uniprotkb:A2AFR8|ensembl:ENSMUSP00000088041	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:sysc_mouse(display_long)|uniprotkb:Sars1(gene name)|psi-mi:Sars1(display_short)|uniprotkb:Sars(gene name synonym)|uniprotkb:Sers(gene name synonym)|uniprotkb:Seryl-tRNA synthetase(gene name synonym)|uniprotkb:"Seryl-tRNA(Ser/Sec) synthetase"(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001191908.1|ensembl:ENSMUSG00000068739(gene)|ensembl:ENSMUST00000090553(transcript)|go:"GO:0000049"(tRNA binding)|go:"GO:0000122"(negative regulation of transcription by RNA polymerase II)|go:"GO:0000978"(RNA polymerase II cis-regulatory region sequence-specific DNA binding)|go:"GO:0004828"(serine-tRNA ligase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005829"(cytosol)|go:"GO:0006434"(seryl-tRNA aminoacylation)|go:"GO:0016525"(negative regulation of angiogenesis)|go:"GO:0019899"(enzyme binding)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0097056"("selenocysteinyl-tRNA(Sec) biosynthetic process")|go:"GO:1904046"(negative regulation of vascular endothelial growth factor production)|interpro:IPR002314("Aminoacyl-tRNA synthetase, class II (G, H, P and S)")|interpro:IPR002317(Seryl-tRNA synthetase, class IIa)|interpro:IPR006195(Aminoacyl-tRNA synthetase, class II)|interpro:IPR010978(tRNA-binding arm)|interpro:IPR015866(Seryl-tRNA synthetase, class IIa, N-terminal)|interpro:IPR033729|interpro:IPR042103|mint:P26638	-	-	comment:mint|function:"Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec)"	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:yRMXbM5tm7RfSScHzlRsHbvoQxI10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q99PL5	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-8361659|uniprotkb:Q99PK6|uniprotkb:Q99PK8|uniprotkb:Q9CS20|uniprotkb:Q99PL4|uniprotkb:Q99PK9|uniprotkb:Q99PL0|intact:MINT-1867743|uniprotkb:Q99PL1|uniprotkb:Q99PL3|uniprotkb:Q99PK7|uniprotkb:Q99PK5|uniprotkb:Q99PL2|uniprotkb:A2AVJ8	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:rrbp1_mouse(display_long)|uniprotkb:Rrbp1(gene name)|psi-mi:Rrbp1(display_short)|uniprotkb:Ribosome receptor protein(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	interpro:IPR040248|refseq:NP_077243.2|refseq:NP_598329.1|mint:Q99PL5|go:"GO:0007165"(signal transduction)|go:"GO:0015031"(protein transport)|go:"GO:0030176"(integral component of endoplasmic reticulum membrane)|interpro:IPR007794(Ribosome receptor lysine or proline rich)	-	-	comment:mint|function:Acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:HSI1U7KA5w2yF41srMqdcd1FM6s10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P62281	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-8328629|uniprotkb:P04643|intact:MINT-1857199|ensembl:ENSMUSP00000003521	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:rs11_mouse(display_long)|uniprotkb:Rps11(gene name)|psi-mi:Rps11(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_038753.1|dip:DIP-59975N|ensembl:ENSMUSG00000003429(gene)|ensembl:ENSMUST00000003521(transcript)|go:"GO:0003735"(structural constituent of ribosome)|go:"GO:0005829"(cytosol)|go:"GO:0006412"(translation)|go:"GO:0019843"(rRNA binding)|go:"GO:0022626"(cytosolic ribosome)|go:"GO:0022627"(cytosolic small ribosomal subunit)|interpro:IPR000266(Ribosomal protein S17)|interpro:IPR012340(Nucleic acid-binding, OB-fold)|interpro:IPR019979|interpro:IPR028333|interpro:IPR032440|mint:P62281|reactome:R-MMU-156827|reactome:R-MMU-1799339|reactome:R-MMU-6791226|reactome:R-MMU-72649|reactome:R-MMU-72689|reactome:R-MMU-72695|reactome:R-MMU-72702|reactome:R-MMU-72706|reactome:R-MMU-975956|reactome:R-MMU-975957	-	-	comment:mint	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:0fqJjtWAuz31VTKt2mbteRoi8vA10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q921J2	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-7924728|intact:MINT-4611176|ensembl:ENSMUSP00000030787	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:rheb_mouse(display_long)|uniprotkb:Rheb(gene name)|psi-mi:Rheb(display_short)|uniprotkb:Ras homolog enriched in brain(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_444305.2|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099175"(regulation of postsynapse organization)|go:"GO:0120163"(negative regulation of cold-induced thermogenesis)|go:"GO:2000074"(regulation of type B pancreatic cell development)|interpro:IPR001806(Ras GTPase)|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR020849|interpro:IPR027417|interpro:IPR037586|mint:Q921J2|rcsb pdb:4O25|rcsb pdb:4O2L|rcsb pdb:4O2R|reactome:R-MMU-1632852|reactome:R-MMU-165159|reactome:R-MMU-166208|reactome:R-MMU-380972|reactome:R-MMU-5628897|reactome:R-MMU-8943724|reactome:R-MMU-9639288|ensembl:ENSMUSG00000028945(gene)|ensembl:ENSMUST00000030787(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000287"(magnesium ion binding)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0014069"(postsynaptic density)|go:"GO:0019003"(GDP binding)|go:"GO:0019901"(protein kinase binding)|go:"GO:0030425"(dendrite)|go:"GO:0032006"(regulation of TOR signaling)|go:"GO:0032008"(positive regulation of TOR signaling)|go:"GO:0043025"(neuronal cell body)|go:"GO:0048714"(positive regulation of oligodendrocyte differentiation)	-	-	function:Not known; binds GTP and exhibits intrinsic GTPase activity|comment:mint	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:hWQL1uMB4w4vwGD6IZBHfOx9UaI10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9D1G1	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-8319000|uniprotkb:Q3U0N1|intact:MINT-1865627|ensembl:ENSMUSP00000025804	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:rab1b_mouse(display_long)|uniprotkb:Rab1b(gene name)|psi-mi:Rab1b(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_083852.1|ensembl:ENSMUSG00000024870(gene)|ensembl:ENSMUST00000025804(transcript)|go:"GO:0000045"(autophagosome assembly)|go:"GO:0003924"(GTPase activity)|go:"GO:0003925"(G protein activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005739"(mitochondrion)|go:"GO:0005793"(endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0012505"(endomembrane system)|go:"GO:0034045"(phagophore assembly site membrane)|go:"GO:0045202"(synapse)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:1903020"(positive regulation of glycoprotein metabolic process)|go:"GO:2000785"(regulation of autophagosome assembly)|interpro:IPR001806(Ras GTPase)|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR027417|mint:Q9D1G1|reactome:R-MMU-162658|reactome:R-MMU-204005|reactome:R-MMU-6807878|reactome:R-MMU-6811434|reactome:R-MMU-8873719|reactome:R-MMU-8876198	-	-	comment:mint|function:Protein transport. Probably involved in vesicular traffic	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:Lyjkc4ttyNLU6U0vbVuQx+7141w10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q8VDQ1	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-8344793|uniprotkb:Q3ULY3|uniprotkb:Q3TG36|uniprotkb:Q9D1W8|intact:UNK-5404747|uniprotkb:Q8BZA2|intact:MINT-4126675|ensembl:ENSMUSP00000115704|ensembl:ENSMUSP00000119981	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:ptgr2_mouse(display_long)|uniprotkb:Zadh1(gene name synonym)|uniprotkb:15-oxoprostaglandin 13-reductase(gene name synonym)|uniprotkb:Zinc-binding alcohol dehydrogenase domain-containing protein 1(gene name synonym)|uniprotkb:Ptgr2(gene name)|psi-mi:Ptgr2(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_084156.2|refseq:NP_001239555.1|refseq:NP_001239554.1|ensembl:ENSMUSG00000072946(gene)|ensembl:ENSMUST00000123614(transcript)|ensembl:ENSMUST00000146377(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005829"(cytosol)|go:"GO:0006693"(prostaglandin metabolic process)|go:"GO:0036132"(13-prostaglandin reductase activity)|go:"GO:0047522"(15-oxoprostaglandin 13-oxidase activity)|interpro:IPR011032(GroES-like)|interpro:IPR013149(Alcohol dehydrogenase, zinc-binding)|interpro:IPR036291|interpro:IPR037399|interpro:IPR041694|mint:Q8VDQ1|rcsb pdb:1VJ1|rcsb pdb:2ZB3	-	-	comment:mint|function:May function as NADPH-dependent oxidoreductase	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:QNea4rx85xekDMhzgPb/VzCO7+010090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P20444	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-6976815|intact:MINT-98140	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:kpca_mouse(display_long)|uniprotkb:Prkca(gene name)|psi-mi:Prkca(display_short)|uniprotkb:Pkca(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	go:"GO:0000302"(response to reactive oxygen species)|go:"GO:0001525"(angiogenesis)|go:"GO:0001750"(photoreceptor outer segment)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0001934"(positive regulation of protein phosphorylation)|go:"GO:0001938"(positive regulation of endothelial cell proliferation)|go:"GO:0002026"(regulation of the force of heart contraction)|go:"GO:0002062"(chondrocyte differentiation)|go:"GO:0002159"(desmosome assembly)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004697"(protein kinase C activity)|go:"GO:0004698"(calcium-dependent protein kinase C activity)|go:"GO:0005178"(integrin binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006469"(negative regulation of protein kinase activity)|go:"GO:0006874"(cellular calcium ion homeostasis)|go:"GO:0006937"(regulation of muscle contraction)|go:"GO:0007155"(cell adhesion)|go:"GO:0007611"(learning or memory)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010360"(negative regulation of anion channel activity)|go:"GO:0010595"(positive regulation of endothelial cell migration)|go:"GO:0010613"(positive regulation of cardiac muscle hypertrophy)|go:"GO:0014704"(intercalated disc)|go:"GO:0016020"(membrane)|go:"GO:0017148"(negative regulation of translation)|go:"GO:0018105"(peptidyl-serine phosphorylation)|go:"GO:0018107"(peptidyl-threonine phosphorylation)|go:"GO:0019899"(enzyme binding)|go:"GO:0021955"(central nervous system neuron axonogenesis)|go:"GO:0030335"(positive regulation of cell migration)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0030593"(neutrophil chemotaxis)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0031966"(mitochondrial membrane)|go:"GO:0032355"(response to estradiol)|go:"GO:0032991"(protein-containing complex)|go:"GO:0034351"(negative regulation of glial cell apoptotic process)|go:"GO:0035403"("histone kinase activity (H3-T6 specific)")|go:"GO:0035408"(histone H3-T6 phosphorylation)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0035866"(alphav-beta3 integrin-PKCalpha complex)|go:"GO:0036289"(peptidyl-serine autophosphorylation)|go:"GO:0043005"(neuron projection)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043409"(negative regulation of MAPK cascade)|go:"GO:0043536"(positive regulation of blood vessel endothelial cell migration)|go:"GO:0031666"(positive regulation of lipopolysaccharide-mediated signaling pathway)|go:"GO:0044305"(calyx of Held)|go:"GO:0045121"(membrane raft)|go:"GO:0045177"(apical part of cell)|go:"GO:0045471"(response to ethanol)|go:"GO:0045651"(positive regulation of macrophage differentiation)|go:"GO:0045766"(positive regulation of angiogenesis)|go:"GO:0045780"(positive regulation of bone resorption)|go:"GO:0045785"(positive regulation of cell adhesion)|go:"GO:0045822"(negative regulation of heart contraction)|go:"GO:0045921"(positive regulation of exocytosis)|go:"GO:0045931"(positive regulation of mitotic cell cycle)|go:"GO:0046325"(negative regulation of glucose import)|go:"GO:0046627"(negative regulation of insulin receptor signaling pathway)|go:"GO:0046777"(protein autophosphorylation)|go:"GO:0047484"(regulation of response to osmotic stress)|go:"GO:0048259"(regulation of receptor-mediated endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0048661"(positive regulation of smooth muscle cell proliferation)|go:"GO:0050729"(positive regulation of inflammatory response)|go:"GO:0050730"(regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050930"(induction of positive chemotaxis)|go:"GO:0051965"(positive regulation of synapse assembly)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0070555"(response to interleukin-1)|go:"GO:0071322"(cellular response to carbohydrate stimulus)|go:"GO:0090330"(regulation of platelet aggregation)|go:"GO:0097193"(intrinsic apoptotic signaling pathway)|go:"GO:0099171"(presynaptic modulation of chemical synaptic transmission)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|go:"GO:2000707"(positive regulation of dense core granule biogenesis)|interpro:IPR000008(C2 calcium-dependent membrane targeting)|interpro:IPR000719(Protein kinase, core)|interpro:IPR000961(Protein kinase, C-terminal)|interpro:IPR002219(Protein kinase C, phorbol ester/diacylglycerol binding)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR014375(Protein kinase C, alpha/beta/gamma types)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR017892|interpro:IPR020454|interpro:IPR034663|interpro:IPR035892|reactome:R-MMU-111933|reactome:R-MMU-114516|reactome:R-MMU-1250196|reactome:R-MMU-1433557|reactome:R-MMU-1433559|reactome:R-MMU-2179392|reactome:R-MMU-3000170|reactome:R-MMU-399997|reactome:R-MMU-416993|reactome:R-MMU-4419969|reactome:R-MMU-450520|reactome:R-MMU-5099900|reactome:R-MMU-5218921|reactome:R-MMU-5668599|reactome:R-MMU-76005|reactome:R-MMU-8853659|refseq:NP_035231.2|mint:P20444|dip:DIP-532N	-	-	comment:mint|function:This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4|function:PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters|function:Expression of the mutant form UV25 causes malignant transformation of cells	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:mcB2Fkcsk/5w7OhEH93JAec585I10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q8CGA0	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-7788270|ensembl:ENSMUSP00000027373|intact:MINT-4115705	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:ppm1f_mouse(display_long)|uniprotkb:Ppm1f(gene name)|psi-mi:Ppm1f(display_short)|uniprotkb:"Ca(2+)/calmodulin-dependent protein kinase phosphatase"(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:XP_017172613.1|ensembl:ENSMUSG00000026181(gene)|ensembl:ENSMUST00000027373(transcript)|go:"GO:0004722"(protein serine/threonine phosphatase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0006469"(negative regulation of protein kinase activity)|go:"GO:0006470"(protein dephosphorylation)|go:"GO:0008138"(protein tyrosine/serine/threonine phosphatase activity)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0010634"(positive regulation of epithelial cell migration)|go:"GO:0010811"(positive regulation of cell-substrate adhesion)|go:"GO:0016576"(histone dephosphorylation)|go:"GO:0030335"(positive regulation of cell migration)|go:"GO:0032991"(protein-containing complex)|go:"GO:0033137"(negative regulation of peptidyl-serine phosphorylation)|go:"GO:0033192"(calmodulin-dependent protein phosphatase activity)|go:"GO:0035690"|go:"GO:0035970"(peptidyl-threonine dephosphorylation)|go:"GO:0043280"(positive regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0044387"(negative regulation of protein kinase activity by regulation of protein phosphorylation)|go:"GO:0045892"(negative regulation of transcription, DNA-templated)|go:"GO:0045927"(positive regulation of growth)|go:"GO:0046872"(metal ion binding)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050921"(positive regulation of chemotaxis)|go:"GO:0051224"(negative regulation of protein transport)|go:"GO:0051496"(positive regulation of stress fiber assembly)|go:"GO:0051894"(positive regulation of focal adhesion assembly)|go:"GO:0070262"(peptidyl-serine dephosphorylation)|go:"GO:0097193"(intrinsic apoptotic signaling pathway)|go:"GO:0106306"|go:"GO:0106307"|go:"GO:1903827"|go:"GO:2000048"(negative regulation of cell-cell adhesion mediated by cadherin)|interpro:IPR000222(Protein phosphatase 2C,  manganese/magnesium aspartate binding site)|interpro:IPR001932(Protein phosphatase 2C-related)|interpro:IPR015655(Protein phosphatase 2C)|interpro:IPR036457|mint:Q8CGA0|refseq:NP_789803.1	-	-	comment:mint|function:Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:264OzmVVef3BKBI9nm8xFsoClh010090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:O35954	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-8327777|intact:MINT-4104925|ensembl:ENSMUSP00000054309|ensembl:ENSMUSP00000097599	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:pitm1_mouse(display_long)|uniprotkb:Pitpnm1(gene name)|psi-mi:Pitpnm1(display_short)|uniprotkb:Dres9(gene name synonym)|uniprotkb:Mpt1(gene name synonym)|uniprotkb:Nir2(gene name synonym)|uniprotkb:Pitpnm(gene name synonym)|uniprotkb:Phosphatidylinositol transfer protein, membrane-associated 1(gene name synonym)|uniprotkb:Mpt-1(gene name synonym)|uniprotkb:Pyk2 N-terminal domain-interacting receptor 2(gene name synonym)|uniprotkb:Drosophila retinal degeneration B homolog 1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_032877.1|ensembl:ENSMUSG00000024851(gene)|ensembl:ENSMUST00000049658(transcript)|ensembl:ENSMUST00000100022(transcript)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005543"(phospholipid binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005811"(lipid droplet)|go:"GO:0005829"(cytosol)|go:"GO:0008525"(phosphatidylcholine transporter activity)|go:"GO:0008526"(phosphatidylinositol transfer activity)|go:"GO:0015031"(protein transport)|go:"GO:0015914"(phospholipid transport)|go:"GO:0030496"(midbody)|go:"GO:0030971"(receptor tyrosine kinase binding)|go:"GO:0031210"(phosphatidylcholine binding)|go:"GO:0032154"(cleavage furrow)|go:"GO:0032580"(Golgi cisterna membrane)|go:"GO:0035091"(phosphatidylinositol binding)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0044297"(cell body)|go:"GO:0070300"(phosphatidic acid binding)|interpro:IPR001666(Phosphatidylinositol transfer protein)|interpro:IPR004177(DDHD)|interpro:IPR023214|interpro:IPR023393|interpro:IPR031315|interpro:IPR036412|mint:O35954|reactome:R-MMU-1483226	-	-	comment:mint|function:"Regulates RHOA activity, and plays a role in cytoskeleton remodeling. Necessary for normal completion of cytokinesis. Plays a role in maintaining normal diacylglycerol levels in the Golgi apparatus. Binds phosphatidyl inositol phosphates (in vitro). May catalyze the transfer of phosphatidylinositol and phosphatidylcholine between membranes (By similarity). Necessary for maintaining the normal structure of the endoplasmic reticulum and the Golgi apparatus. Required for protein export from the endoplasmic reticulum and the Golgi. Binds calcium ions"	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:FOv7BJbaEF2IBuGzhURvp2lPVRs10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q922D8	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-8316499|ensembl:ENSMUSP00000021443|intact:MINT-1856310|uniprotkb:Q8R013	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:c1tc_mouse(display_long)|uniprotkb:Mthfd1(gene name)|psi-mi:Mthfd1(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000021048(gene)|ensembl:ENSMUST00000021443(transcript)|go:"GO:0000105"(histidine biosynthetic process)|go:"GO:0001780"(neutrophil homeostasis)|go:"GO:0001843"(neural tube closure)|go:"GO:0004329"(formate-tetrahydrofolate ligase activity)|go:"GO:0004477"(methenyltetrahydrofolate cyclohydrolase activity)|go:"GO:0004486"("methylenetetrahydrofolate dehydrogenase [NAD(P)+] activity")|go:"GO:0004487"("methylenetetrahydrofolate dehydrogenase (NAD+) activity")|go:"GO:0004488"("methylenetetrahydrofolate dehydrogenase (NADP+) activity")|go:"GO:0005524"(ATP binding)|go:"GO:0005739"(mitochondrion)|go:"GO:0005829"(cytosol)|go:"GO:0006164"(purine nucleotide biosynthetic process)|go:"GO:0006555"(methionine metabolic process)|go:"GO:0006730"(one-carbon metabolic process)|go:"GO:0009069"(serine family amino acid metabolic process)|go:"GO:0009070"(serine family amino acid biosynthetic process)|go:"GO:0009086"(methionine biosynthetic process)|go:"GO:0009257"(10-formyltetrahydrofolate biosynthetic process)|go:"GO:0019346"(transsulfuration)|go:"GO:0035999"(tetrahydrofolate interconversion)|go:"GO:0061053"(somite development)|interpro:IPR000559(Formate-tetrahydrofolate ligase, FTHFS)|interpro:IPR000672(Tetrahydrofolate dehydrogenase/cyclohydrolase)|interpro:IPR020628|interpro:IPR020630|interpro:IPR020631|go:"GO:0007507"(heart development)|interpro:IPR020867|interpro:IPR027417|interpro:IPR036291|mint:Q922D8|reactome:R-MMU-196757|refseq:NP_620084.2	-	-	comment:mint	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:5mhsOKMWX4yCn5OZpAcw9fpf8ac10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q68FL6	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-8587205|ensembl:ENSMUSP00000037446|intact:UNK-5468878|intact:MINT-1869810	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:symc_mouse(display_long)|uniprotkb:Mars1(gene name)|psi-mi:Mars1(display_short)|uniprotkb:Mars(gene name synonym)|uniprotkb:Methionyl-tRNA synthetase(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000040354(gene)|ensembl:ENSMUST00000037290(transcript)|go:"GO:0000049"(tRNA binding)|go:"GO:0004825"(methionine-tRNA ligase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005730"(nucleolus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005829"(cytosol)|go:"GO:0006418"(tRNA aminoacylation for protein translation)|go:"GO:0006431"(methionyl-tRNA aminoacylation)|go:"GO:0009267"(cellular response to starvation)|go:"GO:0009303"(rRNA transcription)|go:"GO:0017101"(aminoacyl-tRNA synthetase multienzyme complex)|go:"GO:0032869"(cellular response to insulin stimulus)|go:"GO:0036120"(cellular response to platelet-derived growth factor stimulus)|go:"GO:0071364"(cellular response to epidermal growth factor stimulus)|go:"GO:1901838"(positive regulation of transcription of nucleolar large rRNA by RNA polymerase I)|interpro:IPR000738(WHEP-TRS)|interpro:IPR001412(Aminoacyl-tRNA synthetase, class I, conserved site)|interpro:IPR004046(Glutathione S-transferase, C-terminal)|interpro:IPR009068(S15/NS1, RNA-binding)|interpro:IPR009080(Aminoacyl-tRNA synthetase, class 1a, anticodon-binding)|interpro:IPR010987(Glutathione S-transferase, C-terminal-like)|interpro:IPR014729(Rossmann-like alpha/beta/alpha sandwich fold)|interpro:IPR014758(Methionyl-tRNA synthetase, class Ia, N-terminal)|interpro:IPR015413("Aminoacyl-tRNA synthetase, class I (M)")|interpro:IPR023458|interpro:IPR029038|interpro:IPR033911|interpro:IPR036282|interpro:IPR041598|interpro:IPR041872|mint:Q68FL6|refseq:NP_001165053.1|refseq:NP_001003913.1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:MzUISmZ5sW5yezibMg19yEv8ny010090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9WU01	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-8339046|uniprotkb:Q6NXZ3|intact:MINT-4131040|ensembl:ENSMUSP00000027226	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:khdr2_mouse(display_long)|uniprotkb:Khdrbs2(gene name)|psi-mi:Khdrbs2(display_short)|uniprotkb:Slm1(gene name synonym)|uniprotkb:Sam68-like mammalian protein 1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_573498.1|ensembl:ENSMUSG00000026058(gene)|ensembl:ENSMUST00000027226(transcript)|go:"GO:0000381"(regulation of alternative mRNA splicing, via spliceosome)|go:"GO:0003729"(mRNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0006397"(mRNA processing)|go:"GO:0008143"("poly(A) binding")|go:"GO:0008266"("poly(U) RNA binding")|go:"GO:0017124"(SH3 domain binding)|go:"GO:0042169"(SH2 domain binding)|go:"GO:0048024"(regulation of mRNA splicing, via spliceosome)|interpro:IPR004087(K Homology)|interpro:IPR004088(K Homology, type 1)|interpro:IPR032335|interpro:IPR032571|interpro:IPR036612|mint:Q9WU01|reactome:R-MMU-8849468	-	-	comment:mint|function:"RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion (By similarity). Its phosphorylation by FYN inhibits its ability to regulate splice site selection (By similarity). Induces an increased concentration-dependent incorporation of exon in CD44 pre-mRNA by direct binding to purine-rich exonic enhancer (By similarity). May function as an adapter protein for Src kinases during mitosis. Binds both poly(A) and poly(U) homopolymers. Phosphorylation by PTK6 inhibits its RNA-binding ability"	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:IVVA2n46neNZhewYfJO16OyDG2g10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q8R366	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-8329630|intact:MINT-4118899|uniprotkb:Q8R0L7	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:igsf8_mouse(display_long)|uniprotkb:Igsf8(gene name)|psi-mi:Igsf8(display_short)|uniprotkb:Ewi2(gene name synonym)|uniprotkb:Kct4(gene name synonym)|uniprotkb:Pgrl(gene name synonym)|uniprotkb:CD81 partner 3(gene name synonym)|uniprotkb:Glu-Trp-Ile EWI motif-containing protein 2(gene name synonym)|uniprotkb:Keratinocyte-associated transmembrane protein 4(gene name synonym)|uniprotkb:Prostaglandin regulatory-like protein(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_536344.1|mint:Q8R366|interpro:IPR036179|interpro:IPR003599(Immunoglobulin subtype)|interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013106(Immunoglobulin V-set)|interpro:IPR013783(Immunoglobulin-like fold)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030424"(axon)|go:"GO:0043209"(myelin sheath)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0045202"(synapse)|go:"GO:2000145"(regulation of cell motility)	-	-	function:"May play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin-dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain"|comment:mint	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:JPeWi6PT7DnIU30GKsrr46Pp2/o10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P48722	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-8314699|ensembl:ENSMUSP00000145468|uniprotkb:Q91X29|intact:MINT-4098038|uniprotkb:P97854|uniprotkb:Q8BQD0|uniprotkb:Q3TQN2|uniprotkb:Q8CC45|uniprotkb:Q3UNG4	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:hs74l_mouse(display_long)|uniprotkb:Hspa4l(gene name)|psi-mi:Hspa4l(display_short)|uniprotkb:Apg1(gene name synonym)|uniprotkb:Hsp4l(gene name synonym)|uniprotkb:Osp94(gene name synonym)|uniprotkb:Osmotic stress protein 94(gene name synonym)|uniprotkb:Heat shock 70-related protein APG-1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000025757(gene)|ensembl:ENSMUST00000204702(transcript)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006457"(protein folding)|go:"GO:0006986"(response to unfolded protein)|interpro:IPR013126(Heat shock protein 70)|interpro:IPR018181|interpro:IPR029047|interpro:IPR029048|interpro:IPR042708|interpro:IPR043129|mint:P48722|reactome:R-MMU-3371453|refseq:XP_006500829.2|refseq:NP_035150.3|refseq:XP_006500830.1	-	-	comment:mint|function:Possesses chaperone activity in vitro where it inhibits aggregation of citrate synthase	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:6woBxNys6oLJnLJERYXaeuymJvw10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P23242	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-298630|ensembl:ENSMUSP00000064536|ensembl:ENSMUSP00000151620|uniprotkb:Q544I7|uniprotkb:Q8CE05	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:cxa1_mouse(display_long)|uniprotkb:Gja1(gene name)|psi-mi:Gja1(display_short)|uniprotkb:Cxn-43(gene name synonym)|uniprotkb:Connexin-43(gene name synonym)|uniprotkb:Gap junction 43 kDa heart protein(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	go:"GO:0005771"(multivesicular body)|go:"GO:0030054"(cell junction)|ensembl:ENSMUSG00000050953(gene)|ensembl:ENSMUST00000068581(transcript)|ensembl:ENSMUST00000220069(transcript)|go:"GO:0000132"(establishment of mitotic spindle orientation)|go:"GO:0001649"(osteoblast differentiation)|go:"GO:0001701"(in utero embryonic development)|go:"GO:0001764"(neuron migration)|go:"GO:0001937"(negative regulation of endothelial cell proliferation)|go:"GO:0001947"(heart looping)|go:"GO:0002070"(epithelial cell maturation)|go:"GO:0002088"(lens development in camera-type eye)|go:"GO:0002544"(chronic inflammatory response)|go:"GO:0002931"(response to ischemia)|go:"GO:0003104"(positive regulation of glomerular filtration)|go:"GO:0003158"(endothelium development)|go:"GO:0003294"(atrial ventricular junction remodeling)|go:"GO:0003347"(epicardial cell to mesenchymal cell transition)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005243"(gap junction channel activity)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005764"(lysosome)|go:"GO:0005768"(endosome)|go:"GO:0005769"(early endosome)|go:"GO:0005770"(late endosome)|go:"GO:0007507"(heart development)|go:"GO:0007512"(adult heart development)|go:"GO:0008013"(beta-catenin binding)|go:"GO:0008016"(regulation of heart contraction)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0008584"(male gonad development)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0009268"(response to pH)|go:"GO:0009749"(response to glucose)|go:"GO:0010232"(vascular transport)|go:"GO:0005882"(intermediate filament)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0010629"(negative regulation of gene expression)|go:"GO:0010643"(cell communication by chemical coupling)|go:"GO:0010644"(cell communication by electrical coupling)|go:"GO:0010649"(regulation of cell communication by electrical coupling)|go:"GO:0005886"(plasma membrane)|go:"GO:0010652"(positive regulation of cell communication by chemical coupling)|go:"GO:0014047"(glutamate secretion)|go:"GO:0014704"(intercalated disc)|go:"GO:0005911"(cell-cell junction)|go:"GO:0005916"(fascia adherens)|go:"GO:0015075"(ion transmembrane transporter activity)|go:"GO:0015562"(efflux transmembrane transporter activity)|go:"GO:0015631"(tubulin binding)|go:"GO:0015867"(ATP transport)|go:"GO:0005921"(gap junction)|go:"GO:0005922"(connexin complex)|go:"GO:0006915"(apoptotic process)|go:"GO:0007154"(cell communication)|go:"GO:0016020"(membrane)|go:"GO:0007165"(signal transduction)|go:"GO:0007204"(positive regulation of cytosolic calcium ion concentration)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016324"(apical plasma membrane)|go:"GO:0016328"(lateral plasma membrane)|go:"GO:0017124"(SH3 domain binding)|go:"GO:0007267"(cell-cell signaling)|go:"GO:0007283"(spermatogenesis)|go:"GO:0019904"(protein domain specific binding)|go:"GO:0022898"(regulation of transmembrane transporter activity)|go:"GO:0030165"(PDZ domain binding)|go:"GO:0030308"(negative regulation of cell growth)|go:"GO:0030500"(regulation of bone mineralization)|go:"GO:0032024"(positive regulation of insulin secretion)|go:"GO:0032277"(negative regulation of gonadotropin secretion)|go:"GO:0032355"(response to estradiol)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032526"(response to retinoic acid)|go:"GO:0032991"(protein-containing complex)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0034405"(response to fluid shear stress)|go:"GO:0034613"(cellular protein localization)|go:"GO:0034634"(glutathione transmembrane transporter activity)|go:"GO:0035050"(embryonic heart tube development)|go:"GO:0035437"(maintenance of protein localization in endoplasmic reticulum)|go:"GO:0042098"(T cell proliferation)|go:"GO:0042110"(T cell activation)|go:"GO:0042311"(vasodilation)|go:"GO:0042733"(embryonic digit morphogenesis)|go:"GO:0042908"(xenobiotic transport)|go:"GO:0042981"(regulation of apoptotic process)|go:"GO:0043014"(alpha-tubulin binding)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0043292"(contractile fiber)|go:"GO:0043403"(skeletal muscle tissue regeneration)|go:"GO:0043434"(response to peptide hormone)|go:"GO:0044291"(cell-cell contact zone)|go:"GO:0045121"(membrane raft)|go:"GO:0071253"(connexin binding)|go:"GO:0071260"(cellular response to mechanical stimulus)|go:"GO:0071374"(cellular response to parathyroid hormone stimulus)|go:"GO:0071467"(cellular response to pH)|go:"GO:0086075"(gap junction channel activity involved in cardiac conduction electrical coupling)|go:"GO:0090162"(establishment of epithelial cell polarity)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0097718"(disordered domain specific binding)|go:"GO:0099111"(microtubule-based transport)|go:"GO:0120162"(positive regulation of cold-induced thermogenesis)|go:"GO:0140115"(export across plasma membrane)|go:"GO:1901164"(negative regulation of trophoblast cell migration)|go:"GO:1903763"(gap junction channel activity involved in cell communication by electrical coupling)|go:"GO:1904646"(cellular response to amyloid-beta)|go:"GO:1904707"(positive regulation of vascular associated smooth muscle cell proliferation)|go:"GO:1905332"(positive regulation of morphogenesis of an epithelium)|go:"GO:1905772"(positive regulation of mesodermal cell differentiation)|go:"GO:0070160"(tight junction)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0045844"(positive regulation of striated muscle tissue development)|go:"GO:0045907"(positive regulation of vasoconstriction)|go:"GO:0046697"(decidualization)|go:"GO:0046849"(bone remodeling)|go:"GO:0046850"(regulation of bone remodeling)|go:"GO:0048487"(beta-tubulin binding)|go:"GO:0048514"(blood vessel morphogenesis)|go:"GO:0048812"(neuron projection morphogenesis)|go:"GO:0051924"(regulation of calcium ion transport)|go:"GO:0055077"(gap junction hemi-channel activity)|go:"GO:0055085"(transmembrane transport)|go:"GO:0060044"(negative regulation of cardiac muscle cell proliferation)|go:"GO:0060156"(milk ejection reflex)|go:"GO:0060307"(regulation of ventricular cardiac muscle cell membrane repolarization)|go:"GO:0060312"(regulation of blood vessel remodeling)|go:"GO:0060348"(bone development)|go:"GO:0060371"(regulation of atrial cardiac muscle cell membrane depolarization)|go:"GO:0060373"(regulation of ventricular cardiac muscle cell membrane depolarization)|go:"GO:0061045"(negative regulation of wound healing)|go:"GO:0061337"(cardiac conduction)|go:"GO:0045216"(cell-cell junction organization)|go:"GO:1990782"(protein tyrosine kinase binding)|go:"GO:2000279"(negative regulation of DNA biosynthetic process)|go:"GO:2000648"(positive regulation of stem cell proliferation)|go:"GO:2000810"(regulation of bicellular tight junction assembly)|go:"GO:2000987"(positive regulation of behavioral fear response)|interpro:IPR000500(Connexins)|interpro:IPR002261("Gap junction alpha-1 protein (Cx43)")|interpro:IPR013092(Connexin, N-terminal)|interpro:IPR013124("Gap junction alpha-1 protein (Cx43), C-terminal")|interpro:IPR017990|interpro:IPR019570|interpro:IPR034634|interpro:IPR035091|interpro:IPR038359|mint:P23242|reactome:R-MMU-190840|reactome:R-MMU-190861|reactome:R-MMU-190873|reactome:R-MMU-191650|reactome:R-MMU-196025|reactome:R-MMU-9013406|go:"GO:1905867"(epididymis development)|refseq:NP_034418.1|dip:DIP-29207N	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:y7Yg1+W6tlVZnaRALQBCWQppVf010090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q68FF6	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-645933|ensembl:ENSMUSP00000037210	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:git1_mouse(display_long)|uniprotkb:G protein-coupled receptor kinase-interactor 1(gene name synonym)|uniprotkb:GRK-interacting protein 1(gene name synonym)|uniprotkb:Git1(gene name)|psi-mi:Git1(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001004144.1|ensembl:ENSMUSG00000011877(gene)|ensembl:ENSMUST00000037285(transcript)|go:"GO:0001771"(immunological synapse formation)|go:"GO:0001957"(intramembranous ossification)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0005768"(endosome)|go:"GO:0005813"(centrosome)|go:"GO:0005829"(cytosol)|go:"GO:0005925"(focal adhesion)|go:"GO:0007420"(brain development)|go:"GO:0007626"(locomotory behavior)|go:"GO:0008277"(regulation of G protein-coupled receptor signaling pathway)|go:"GO:0014069"(postsynaptic density)|go:"GO:0030027"(lamellipodium)|go:"GO:0031267"(small GTPase binding)|go:"GO:0032013"(negative regulation of ARF protein signal transduction)|go:"GO:0032465"(regulation of cytokinesis)|go:"GO:0032691"(negative regulation of interleukin-1 beta production)|go:"GO:0042802"(identical protein binding)|go:"GO:0043005"(neuron projection)|go:"GO:0043015"(gamma-tubulin binding)|go:"GO:0044305"(calyx of Held)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045202"(synapse)|go:"GO:0045454"(cell redox homeostasis)|go:"GO:0045820"(negative regulation of glycolytic process)|go:"GO:0046872"(metal ion binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0048666"(neuron development)|go:"GO:0060996"(dendritic spine development)|go:"GO:0061743"(motor learning)|go:"GO:0071222"(cellular response to lipopolysaccharide)|go:"GO:0071364"(cellular response to epidermal growth factor stimulus)|go:"GO:0090063"(positive regulation of microtubule nucleation)|go:"GO:0097431"(mitotic spindle pole)|go:"GO:0098794"(postsynapse)|go:"GO:0098879"(structural constituent of postsynaptic specialization)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099171"(presynaptic modulation of chemical synaptic transmission)|go:"GO:0099645"(neurotransmitter receptor localization to postsynaptic specialization membrane)|go:"GO:0106015"(negative regulation of inflammatory response to wounding)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|go:"GO:2000646"(positive regulation of receptor catabolic process)|interpro:IPR001164(Arf GTPase activating protein)|interpro:IPR002110(Ankyrin)|interpro:IPR013724("Spa2 homology (SHD) of GIT")|interpro:IPR020683|interpro:IPR022018|interpro:IPR032352|interpro:IPR036770|interpro:IPR037278|interpro:IPR038508|mint:Q68FF6|rcsb pdb:6JMU|reactome:R-MMU-3928664|reactome:R-MMU-9013148|reactome:R-MMU-9013149|reactome:R-MMU-9013404|reactome:R-MMU-9013406|reactome:R-MMU-9013420|reactome:R-MMU-9013423|reactome:R-MMU-9013424	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:3br9Xz6GZQV409u3u7CZDfqKMIM10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q60902	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-443931|ensembl:ENSMUSP00000129739|uniprotkb:Q3U7L9|uniprotkb:Q8CB60|uniprotkb:Q8CB70|uniprotkb:Q3UIS9|uniprotkb:Q91WH8	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:ep15r_mouse(display_long)|uniprotkb:Eps15-related protein(gene name synonym)|uniprotkb:Epidermal growth factor receptor pathway substrate 15-related sequence(gene name synonym)|uniprotkb:Eps15l1(gene name)|psi-mi:Eps15l1(display_short)|uniprotkb:Eps15-rs(gene name synonym)|uniprotkb:Eps15R(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000006276(gene)|ensembl:ENSMUST00000163643(transcript)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005634"(nucleus)|go:"GO:0006897"(endocytosis)|go:"GO:0016197"(endosomal transport)|go:"GO:0030132"(clathrin coat of coated pit)|interpro:IPR000261("EPS15 homology (EH)")|interpro:IPR002048(Calcium-binding EF-hand)|interpro:IPR003903(Ubiquitin interacting motif)|interpro:IPR011992(EF-Hand type)|interpro:IPR018247|mint:Q60902|reactome:R-MMU-182971|reactome:R-MMU-8856825|reactome:R-MMU-8856828|refseq:NP_001116304.1|refseq:NP_031970.2|refseq:NP_001276788.1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:8Xag/aZPFGDnwUbio3gkHL3nMes10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q99L45	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-2527165|uniprotkb:Q9CSH6|uniprotkb:Q9CT12|ensembl:ENSMUSP00000096777|ensembl:ENSMUSP00000128257	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:if2b_mouse(display_long)|uniprotkb:Eif2s2(gene name)|psi-mi:Eif2s2(display_short)|uniprotkb:Eukaryotic translation initiation factor 2 subunit beta(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_080306.1|ensembl:ENSMUSG00000074656(gene)|ensembl:ENSMUST00000099173(transcript)|ensembl:ENSMUST00000166171(transcript)|go:"GO:0001701"(in utero embryonic development)|go:"GO:0001731"(formation of translation preinitiation complex)|go:"GO:0001732"(formation of cytoplasmic translation initiation complex)|go:"GO:0002176"(male germ cell proliferation)|go:"GO:0003729"(mRNA binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005850"(eukaryotic translation initiation factor 2 complex)|go:"GO:0008584"(male gonad development)|go:"GO:0031369"(translation initiation factor binding)|go:"GO:0046872"(metal ion binding)|interpro:IPR002735(Translation initiation factor IF2/IF5)|interpro:IPR016189(Translation initiation factor IF2/IF5, N-terminal)|interpro:IPR016190(Translation initiation factor IF2/IF5, zinc-binding)|mint:Q99L45|reactome:R-MMU-156827|reactome:R-MMU-381042|reactome:R-MMU-382556|reactome:R-MMU-72649|reactome:R-MMU-72695|reactome:R-MMU-72702|reactome:R-MMU-72706|reactome:R-MMU-72731	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:rFKfeTTSJtJOwP6jsL1OCSQKsCU10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q6ZQ18	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-26595870|ensembl:ENSMUSP00000106809|uniprotkb:B2RXT1	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:efr3b_mouse(display_long)|uniprotkb:Efr3b(gene name)|psi-mi:Efr3b(display_short)|uniprotkb:Kiaa0953(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000020658(gene)|ensembl:ENSMUST00000111178(transcript)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0072659"(protein localization to plasma membrane)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|mint:Q6ZQ18|refseq:NP_001075952.1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:4dVY77QIwmVMY4pbRQtoJJ5fiLU10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P39054	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-642337|uniprotkb:Q9DBE1|ensembl:ENSMUSP00000133564	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:dyn2_mouse(display_long)|uniprotkb:Dynamin UDNM(gene name synonym)|uniprotkb:Dnm2(gene name)|psi-mi:Dnm2(display_short)|uniprotkb:Dyn2(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001240822.1|refseq:NP_001240823.1|refseq:NP_031897.2|ensembl:ENSMUSG00000033335(gene)|ensembl:ENSMUST00000172482(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000266"(mitochondrial fission)|go:"GO:0001891"(phagocytic cup)|go:"GO:0001917"(photoreceptor inner segment)|go:"GO:0002031"(G protein-coupled receptor internalization)|go:"GO:0003281"(ventricular septum development)|go:"GO:0003374"(dynamin family protein polymerization involved in mitochondrial fission)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005768"(endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005813"(centrosome)|go:"GO:0005829"(cytosol)|go:"GO:0005874"(microtubule)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0006893"(Golgi to plasma membrane transport)|go:"GO:0006897"(endocytosis)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0006909"(phagocytosis)|go:"GO:0008017"(microtubule binding)|go:"GO:0010592"(positive regulation of lamellipodium assembly)|go:"GO:0014069"(postsynaptic density)|go:"GO:0015630"(microtubule cytoskeleton)|go:"GO:0016020"(membrane)|go:"GO:0016185"(synaptic vesicle budding from presynaptic endocytic zone membrane)|go:"GO:0017124"(SH3 domain binding)|go:"GO:0019901"(protein kinase binding)|go:"GO:0030027"(lamellipodium)|go:"GO:0030054"(cell junction)|go:"GO:0030424"(axon)|go:"GO:0030496"(midbody)|go:"GO:0030512"(negative regulation of transforming growth factor beta receptor signaling pathway)|go:"GO:0030516"(regulation of axon extension)|go:"GO:0030670"(phagocytic vesicle membrane)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031623"(receptor internalization)|go:"GO:0031749"(D2 dopamine receptor binding)|go:"GO:0031966"(mitochondrial membrane)|go:"GO:0060976"(coronary vasculature development)|go:"GO:0061025"(membrane fusion)|go:"GO:0098793"(presynapse)|go:"GO:0098844"(postsynaptic endocytic zone membrane)|go:"GO:0098884"(postsynaptic neurotransmitter receptor internalization)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:1900026"(positive regulation of substrate adhesion-dependent cell spreading)|go:"GO:1902856"(negative regulation of non-motile cilium assembly)|go:"GO:1903351"(cellular response to dopamine)|go:"GO:1903358"(regulation of Golgi organization)|go:"GO:1903408"("positive regulation of P-type sodium:potassium-exchanging transporter activity")|go:"GO:1903526"(negative regulation of membrane tubulation)|go:"GO:0032587"(ruffle membrane)|go:"GO:0033572"(transferrin transport)|go:"GO:0035020"(regulation of Rac protein signal transduction)|go:"GO:0035904"(aorta development)|go:"GO:0036312"(phosphatidylinositol 3-kinase regulatory subunit binding)|go:"GO:0044327"(dendritic spine head)|go:"GO:0044351"(macropinocytosis)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0045334"(clathrin-coated endocytic vesicle)|go:"GO:0045429"(positive regulation of nitric oxide biosynthetic process)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0048488"(synaptic vesicle endocytosis)|go:"GO:0032991"(protein-containing complex)|go:"GO:0048812"(neuron projection morphogenesis)|go:"GO:0050699"(WW domain binding)|go:"GO:0050766"(positive regulation of phagocytosis)|go:"GO:0050803"(regulation of synapse structure or activity)|go:"GO:0050998"(nitric-oxide synthase binding)|go:"GO:2000370"(positive regulation of clathrin-dependent endocytosis)|interpro:IPR000375(Dynamin central region)|interpro:IPR001401(Dynamin, GTPase region)|interpro:IPR001849(Pleckstrin-like)|interpro:IPR003130(Dynamin GTPase effector)|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR019762|interpro:IPR020850|interpro:IPR022812|interpro:IPR027188|interpro:IPR027417|interpro:IPR030381|mint:P39054|reactome:R-MMU-166016|reactome:R-MMU-190873|reactome:R-MMU-196025|reactome:R-MMU-203641|reactome:R-MMU-2132295|reactome:R-MMU-432720|reactome:R-MMU-432722|reactome:R-MMU-437239|reactome:R-MMU-8856828	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:+f91mVsvYvuhsUOSzw37RpZv/2M10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9D2G2	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-773210|uniprotkb:Q3UEA0|uniprotkb:Q4FK55|uniprotkb:Q8CIE8|ensembl:ENSMUSP00000060346|ensembl:ENSMUSP00000152664	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:odo2_mouse(display_long)|uniprotkb:Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex(gene name synonym)|uniprotkb:E2K(gene name synonym)|uniprotkb:Dlst(gene name)|psi-mi:Dlst(display_short)|uniprotkb:2-oxoglutarate dehydrogenase complex component E2(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_084501.1|ensembl:ENSMUSG00000004789(gene)|ensembl:ENSMUST00000053811(transcript)|ensembl:ENSMUST00000221357(transcript)|go:"GO:0004149"(dihydrolipoyllysine-residue succinyltransferase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005759"(mitochondrial matrix)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006099"(tricarboxylic acid cycle)|go:"GO:0006103"(2-oxoglutarate metabolic process)|go:"GO:0006104"(succinyl-CoA metabolic process)|go:"GO:0006734"(NADH metabolic process)|go:"GO:0016746"(acyltransferase activity)|go:"GO:0031072"(heat shock protein binding)|go:"GO:0033512"(L-lysine catabolic process to acetyl-CoA via saccharopine)|go:"GO:0034451"(centriolar satellite)|go:"GO:0043209"(myelin sheath)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0045252"(oxoglutarate dehydrogenase complex)|go:"GO:0051087"(chaperone binding)|go:"GO:0106077"(histone succinylation)|interpro:IPR000089(Biotin/lipoyl attachment)|interpro:IPR001078(Catalytic domain of components of various dehydrogenase complexes)|interpro:IPR003016(2-oxo acid dehydrogenase, lipoyl-binding site)|interpro:IPR006255(Dihydrolipoamide succinyltransferase)|interpro:IPR011053(Single hybrid motif)|interpro:IPR023213|mint:Q9D2G2|reactome:R-MMU-389661|reactome:R-MMU-71064|reactome:R-MMU-71403	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:jyBoApRkfA3STOc5qJ01/cE2UHU10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q6PFD5	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-2308733|uniprotkb:Q6PDX0|uniprotkb:Q6XBF2|ensembl:ENSMUSP00000039724|ensembl:ENSMUSP00000101700	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:dlgp3_mouse(display_long)|uniprotkb:Dlgap3(gene name)|psi-mi:Dlgap3(display_short)|uniprotkb:Dap3(gene name synonym)|uniprotkb:SAP90/PSD-95-associated protein 3(gene name synonym)|uniprotkb:PSD-95/SAP90-binding protein 3(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:XP_011238839.1|refseq:NP_941020.1|refseq:NP_001289010.1|ensembl:ENSMUSG00000042388(gene)|ensembl:ENSMUST00000046659(transcript)|ensembl:ENSMUST00000106094(transcript)|go:"GO:0001540"(amyloid-beta binding)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0019904"(protein domain specific binding)|go:"GO:0023052"(signaling)|go:"GO:0030165"(PDZ domain binding)|go:"GO:0031594"(neuromuscular junction)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043197"(dendritic spine)|go:"GO:0045202"(synapse)|go:"GO:0060090"(molecular adaptor activity)|go:"GO:0065003"(protein-containing complex assembly)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0098962"(regulation of postsynaptic neurotransmitter receptor activity)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098981"(cholinergic synapse)|go:"GO:0099563"(modification of synaptic structure)|go:"GO:0099572"(postsynaptic specialization)|interpro:IPR005026(Guanylate-kinase-associated protein)|interpro:IPR030526|mint:Q6PFD5|rcsb pdb:5IZU|reactome:R-MMU-6794361	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:mkXYwYR5Snou4RA8d71UDC7G59I10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q62108	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-300895|ensembl:ENSMUSP00000104229|uniprotkb:Q5NCV5|uniprotkb:Q5NCV6|uniprotkb:Q5NCV7|uniprotkb:Q91WJ1	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:dlg4_mouse(display_long)|uniprotkb:Dlg4(gene name)|psi-mi:Dlg4(display_short)|uniprotkb:Dlgh4(gene name synonym)|uniprotkb:Psd95(gene name synonym)|uniprotkb:Postsynaptic density protein 95(gene name synonym)|uniprotkb:Synapse-associated protein 90(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000020886(gene)|ensembl:ENSMUST00000108588(transcript)|go:"GO:0002091"(negative regulation of receptor internalization)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005109"(frizzled binding)|go:"GO:0005198"(structural molecule activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005886"(plasma membrane)|go:"GO:0005911"(cell-cell junction)|go:"GO:0007204"(positive regulation of cytosolic calcium ion concentration)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007626"(locomotory behavior)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0014069"(postsynaptic density)|go:"GO:0016020"(membrane)|go:"GO:0016188"(synaptic vesicle maturation)|go:"GO:0019865"(immunoglobulin binding)|go:"GO:0019894"(kinesin binding)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0019900"(kinase binding)|go:"GO:0019901"(protein kinase binding)|go:"GO:0019903"(protein phosphatase binding)|go:"GO:0030054"(cell junction)|go:"GO:0030165"(PDZ domain binding)|go:"GO:0030425"(dendrite)|go:"GO:0030863"(cortical cytoskeleton)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0031594"(neuromuscular junction)|go:"GO:0031697"(beta-1 adrenergic receptor binding)|go:"GO:0031698"(beta-2 adrenergic receptor binding)|go:"GO:0031748"(D1 dopamine receptor binding)|go:"GO:0031812"(P2Y1 nucleotide receptor binding)|go:"GO:0032281"(AMPA glutamate receptor complex)|go:"GO:0032839"(dendrite cytoplasm)|go:"GO:0033130"(acetylcholine receptor binding)|go:"GO:0035176"(social behavior)|go:"GO:0035254"(glutamate receptor binding)|go:"GO:0035255"(ionotropic glutamate receptor binding)|go:"GO:0035418"(protein localization to synapse)|go:"GO:0035641"(locomotory exploration behavior)|go:"GO:0035865"(cellular response to potassium ion)|go:"GO:0042043"(neurexin family protein binding)|go:"GO:0042220"(response to cocaine)|go:"GO:0043005"(neuron projection)|go:"GO:0043113"(receptor clustering)|go:"GO:0043197"(dendritic spine)|go:"GO:0044224"(juxtaparanode region of axon)|go:"GO:0044300"(cerebellar mossy fiber)|go:"GO:0044306"(neuron projection terminus)|go:"GO:0044309"(neuron spine)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045184"(establishment of protein localization)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0048168"(regulation of neuronal synaptic plasticity)|go:"GO:0048169"(regulation of long-term neuronal synaptic plasticity)|go:"GO:0050806"(positive regulation of synaptic transmission)|go:"GO:0050885"(neuromuscular process controlling balance)|go:"GO:0060997"(dendritic spine morphogenesis)|go:"GO:0061098"(positive regulation of protein tyrosine kinase activity)|go:"GO:0065003"(protein-containing complex assembly)|go:"GO:0071625"(vocalization behavior)|go:"GO:0071944"(cell periphery)|go:"GO:0097060"(synaptic membrane)|go:"GO:0097061"(dendritic spine organization)|go:"GO:0097109"(neuroligin family protein binding)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0097113"(AMPA glutamate receptor clustering)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098609"(cell-cell adhesion)|go:"GO:0098794"(postsynapse)|go:"GO:0098839"(postsynaptic density membrane)|go:"GO:0098919"(structural constituent of postsynaptic density)|go:"GO:0098970"(postsynaptic neurotransmitter receptor diffusion trapping)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099031"(anchored component of postsynaptic density membrane)|go:"GO:0099645"(neurotransmitter receptor localization to postsynaptic specialization membrane)|go:"GO:0150012"(positive regulation of neuron projection arborization)|go:"GO:1904719"(positive regulation of AMPA glutamate receptor clustering)|go:"GO:2000310"(regulation of NMDA receptor activity)|go:"GO:2000463"(positive regulation of excitatory postsynaptic potential)|go:"GO:2000821"(regulation of grooming behavior)|go:"GO:0060076"(excitatory synapse)|interpro:IPR001452(Src homology-3)|interpro:IPR008145(Guanylate kinase/L-type calcium channel region)|interpro:IPR001478(PDZ/DHR/GLGF)|interpro:IPR008144(Guanylate kinase)|interpro:IPR019583|interpro:IPR019590|interpro:IPR020590|interpro:IPR027417|interpro:IPR036028|interpro:IPR036034|mint:Q62108|reactome:R-MMU-399719|reactome:R-MMU-438066|reactome:R-MMU-451308|reactome:R-MMU-5625900|reactome:R-MMU-5673001|reactome:R-MMU-5682910|reactome:R-MMU-6794361|reactome:R-MMU-8849932|interpro:IPR016313("Membrane-associated guanylate kinase (MAGUK) scaffold protein")|refseq:NP_001103222.1|refseq:NP_031890.1|dip:DIP-29888N	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:9Ylvs2goE8Z/6ff4AqFCEKrPanY10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:O08749	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-773199|uniprotkb:Q3TG55|uniprotkb:Q3U5W5|uniprotkb:Q3UWP7|uniprotkb:Q99LD3|ensembl:ENSMUSP00000106481	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:dldh_mouse(display_long)|uniprotkb:Dihydrolipoamide dehydrogenase(gene name synonym)|uniprotkb:Dld(gene name)|psi-mi:Dld(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_031887.2|ensembl:ENSMUSG00000020664(gene)|ensembl:ENSMUST00000110857(transcript)|go:"GO:0004148"(dihydrolipoyl dehydrogenase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005759"(mitochondrial matrix)|go:"GO:0005929"(cilium)|go:"GO:0006086"(acetyl-CoA biosynthetic process from pyruvate)|go:"GO:0006103"(2-oxoglutarate metabolic process)|go:"GO:0006120"(mitochondrial electron transport, NADH to ubiquinone)|go:"GO:0006508"(proteolysis)|go:"GO:0007369"(gastrulation)|go:"GO:0007568"(aging)|go:"GO:0009106"(lipoate metabolic process)|go:"GO:0031514"(motile cilium)|go:"GO:0034604"("pyruvate dehydrogenase (NAD+) activity")|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0043159"(acrosomal matrix)|go:"GO:0043209"(myelin sheath)|go:"GO:0043544"(lipoamide binding)|go:"GO:0045252"(oxoglutarate dehydrogenase complex)|go:"GO:0045254"(pyruvate dehydrogenase complex)|go:"GO:0045454"(cell redox homeostasis)|go:"GO:0048240"(sperm capacitation)|go:"GO:0050660"(flavin adenine dinucleotide binding)|go:"GO:0051068"(dihydrolipoamide metabolic process)|go:"GO:0051287"(NAD binding)|go:"GO:0061732"(mitochondrial acetyl-CoA biosynthetic process from pyruvate)|go:"GO:0106077"(histone succinylation)|interpro:IPR001100(Pyridine nucleotide-disulphide oxidoreductase, class I)|interpro:IPR004099(Pyridine nucleotide-disulphide oxidoreductase, dimerisation)|interpro:IPR006258(Dihydrolipoamide dehydrogenase)|interpro:IPR012999(Pyridine nucleotide-disulphide oxidoreductase, class I, active site)|interpro:IPR016156(FAD/NAD-linked reductase, dimerisation)|interpro:IPR023753|interpro:IPR036188|mint:O08749|reactome:R-MMU-204174|reactome:R-MMU-389661|reactome:R-MMU-5362517|reactome:R-MMU-6783984|reactome:R-MMU-70268|reactome:R-MMU-70895|reactome:R-MMU-71064|reactome:R-MMU-71403	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:6j/8rw4lkNMW4Lo5sGoRdDJBk2w10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q8BMF4	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-769141|uniprotkb:Q8K2G8|uniprotkb:Q8R339|uniprotkb:Q91ZB1|ensembl:ENSMUSP00000034567	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:odp2_mouse(display_long)|uniprotkb:Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex(gene name synonym)|uniprotkb:Pyruvate dehydrogenase complex component E2(gene name synonym)|uniprotkb:Dlat(gene name)|psi-mi:Dlat(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_663589.3|ensembl:ENSMUSG00000000168(gene)|ensembl:ENSMUST00000034567(transcript)|go:"GO:0004742"(dihydrolipoyllysine-residue acetyltransferase activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0005967"(mitochondrial pyruvate dehydrogenase complex)|go:"GO:0006006"(glucose metabolic process)|go:"GO:0006086"(acetyl-CoA biosynthetic process from pyruvate)|go:"GO:0006099"(tricarboxylic acid cycle)|go:"GO:0030431"(sleep)|go:"GO:0034604"("pyruvate dehydrogenase (NAD+) activity")|go:"GO:0042802"(identical protein binding)|go:"GO:0043209"(myelin sheath)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0045254"(pyruvate dehydrogenase complex)|go:"GO:0061732"(mitochondrial acetyl-CoA biosynthetic process from pyruvate)|interpro:IPR000089(Biotin/lipoyl attachment)|interpro:IPR001078(Catalytic domain of components of various dehydrogenase complexes)|interpro:IPR003016(2-oxo acid dehydrogenase, lipoyl-binding site)|interpro:IPR004167(E3 binding)|interpro:IPR006257(Dihydrolipoamide acetyltransferase, long form)|interpro:IPR011053(Single hybrid motif)|interpro:IPR023213|interpro:IPR036625|mint:Q8BMF4|reactome:R-MMU-204174|reactome:R-MMU-389661|reactome:R-MMU-5362517|reactome:R-MMU-70268	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:I0x+9L5a4O6Bch+NFbNzYiONj+810090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q62095	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-26595844|ensembl:ENSMUSP00000088729|uniprotkb:Q9QWS9	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:ddx3y_mouse(display_long)|uniprotkb:D1Pas1-related sequence 1(gene name synonym)|uniprotkb:DEAD box protein 3, Y-chromosomal(gene name synonym)|uniprotkb:DEAD-box RNA helicase DEAD2(gene name synonym)|uniprotkb:Ddx3y(gene name)|psi-mi:Ddx3y(display_short)|uniprotkb:D1Pas1-rs1(gene name synonym)|uniprotkb:Dead2(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000069045(gene)|ensembl:ENSMUST00000091190(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003724"(RNA helicase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0007276"(gamete generation)|go:"GO:0030154"(cell differentiation)|go:"GO:0043186"(P granule)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|interpro:IPR000629(RNA helicase, ATP-dependent, DEAD-box, conserved site)|interpro:IPR001650(DNA/RNA helicase, C-terminal)|interpro:IPR011545(DNA/RNA helicase, DEAD/DEAH box type, N-terminal)|interpro:IPR014001(DEAD-like helicase, N-terminal)|interpro:IPR014014(RNA helicase, DEAD-box type, Q motif)|interpro:IPR027417|mint:Q62095|refseq:NP_036138.1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:uIyY3/8vJ6qqOtJJ8Hwu4K+OpIU10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q8CBY8	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-776033|uniprotkb:Q923A0|uniprotkb:Q9D4X0|ensembl:ENSMUSP00000153008	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:dctn4_mouse(display_long)|uniprotkb:Dynactin subunit p62(gene name synonym)|uniprotkb:Dctn4(gene name)|psi-mi:Dctn4(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_080578.1|refseq:XP_006526252.1|ensembl:ENSMUSG00000024603(gene)|ensembl:ENSMUST00000223984(transcript)|go:"GO:0000776"(kinetochore)|go:"GO:0000922"(spindle pole)|go:"GO:0001725"(stress fiber)|go:"GO:0005813"(centrosome)|go:"GO:0005868"(cytoplasmic dynein complex)|go:"GO:0005869"(dynactin complex)|go:"GO:0005925"(focal adhesion)|go:"GO:0005938"(cell cortex)|go:"GO:0030017"(sarcomere)|go:"GO:0047485"(protein N-terminus binding)|interpro:IPR008603(Dynactin p62)|mint:Q8CBY8|reactome:R-MMU-2132295|reactome:R-MMU-3371497|reactome:R-MMU-6807878|reactome:R-MMU-6811436	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:ku/Cy0u7rZmYNcJ19nL9Mn6YLYQ10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9JLM8	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-771719|uniprotkb:Q6P207|ensembl:ENSMUSP00000050034	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:dclk1_mouse(display_long)|uniprotkb:Doublecortin-like and CAM kinase-like 1(gene name synonym)|uniprotkb:Dclk1(gene name)|psi-mi:Dclk1(display_short)|uniprotkb:Dclk(gene name synonym)|uniprotkb:Dcamkl1(gene name synonym)|uniprotkb:Doublecortin-like kinase 1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_064362.1|ensembl:ENSMUSG00000027797(gene)|ensembl:ENSMUST00000054237(transcript)|go:"GO:0001764"(neuron migration)|go:"GO:0004672"(protein kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0007409"(axonogenesis)|go:"GO:0007420"(brain development)|go:"GO:0009615"(response to virus)|go:"GO:0014069"(postsynaptic density)|go:"GO:0018105"(peptidyl-serine phosphorylation)|go:"GO:0021952"(central nervous system projection neuron axonogenesis)|go:"GO:0030900"(forebrain development)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0048675"(axon extension)|go:"GO:0048812"(neuron projection morphogenesis)|go:"GO:0048813"(dendrite morphogenesis)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1900181"(negative regulation of protein localization to nucleus)|interpro:IPR000719(Protein kinase, core)|interpro:IPR003533(Doublecortin)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR036572|mint:Q9JLM8	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:P3ZZ2GyKcdMimAcekZ8OlLbuciM10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P70211	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-1798863|uniprotkb:G3X9X6|ensembl:ENSMUSP00000110593	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:dcc_mouse(display_long)|uniprotkb:Dcc(gene name)|psi-mi:Dcc(display_short)|uniprotkb:Tumor suppressor protein DCC(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:XP_006525651.1|refseq:NP_031857.2|dip:DIP-46587N|ensembl:ENSMUSG00000060534(gene)|ensembl:ENSMUST00000114943(transcript)|go:"GO:0001764"(neuron migration)|go:"GO:0003713"(transcription coactivator activity)|go:"GO:0005042"(netrin receptor activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0006915"(apoptotic process)|go:"GO:0007411"(axon guidance)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0021965"(spinal cord ventral commissure morphogenesis)|go:"GO:0030424"(axon)|go:"GO:0032584"(growth cone membrane)|go:"GO:0033563"(dorsal/ventral axon guidance)|go:"GO:0033564"(anterior/posterior axon guidance)|go:"GO:0042802"(identical protein binding)|go:"GO:0045121"(membrane raft)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0099061"(integral component of postsynaptic density membrane)|go:"GO:0099170"(postsynaptic modulation of chemical synaptic transmission)|go:"GO:1901214"(regulation of neuron death)|interpro:IPR003598(Immunoglobulin subtype 2)|interpro:IPR003599(Immunoglobulin subtype)|interpro:IPR003961(Fibronectin, type III)|interpro:IPR007110(Immunoglobulin-like)|interpro:IPR010560(Neogenin, C-terminal)|interpro:IPR013098(Immunoglobulin I-set)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR033012|interpro:IPR036116|interpro:IPR036179|mint:P70211|rcsb pdb:3PZD|rcsb pdb:4PLO|reactome:R-MMU-373752|reactome:R-MMU-418885|reactome:R-MMU-418889	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:EMyR8LhWLCvJIBIAEn9dULe8Xfo10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q62418	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-1550322|uniprotkb:Q80WP1|uniprotkb:Q8BH56|uniprotkb:Q3TG34|uniprotkb:Q3U5X3|uniprotkb:Q3U8I5|uniprotkb:Q3UZ33|uniprotkb:Q5NCI5|uniprotkb:Q5NCI6|uniprotkb:Q5NCI7|ensembl:ENSMUSP00000020769	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:dbnl_mouse(display_long)|uniprotkb:Dbnl(gene name)|psi-mi:Dbnl(display_short)|uniprotkb:Abp1(gene name synonym)|uniprotkb:Sh3p7(gene name synonym)|uniprotkb:SH3 domain-containing protein 7(gene name synonym)|uniprotkb:Actin-binding protein 1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001139780.1|refseq:NP_001139781.1|refseq:NP_038838.1|dip:DIP-39835N|ensembl:ENSMUSG00000020476(gene)|ensembl:ENSMUST00000020769(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001726"(ruffle)|go:"GO:0002102"(podosome)|go:"GO:0002250"(adaptive immune response)|go:"GO:0003779"(actin binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005769"(early endosome)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005938"(cell cortex)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0007416"(synapse assembly)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0014069"(postsynaptic density)|go:"GO:0016601"(Rac protein signal transduction)|go:"GO:0019904"(protein domain specific binding)|go:"GO:0030027"(lamellipodium)|go:"GO:0030425"(dendrite)|go:"GO:0030427"(site of polarized growth)|go:"GO:0030665"(clathrin-coated vesicle membrane)|go:"GO:0030833"(regulation of actin filament polymerization)|go:"GO:0030864"(cortical actin cytoskeleton)|go:"GO:0043204"(perikaryon)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0045773"(positive regulation of axon extension)|go:"GO:0048812"(neuron projection morphogenesis)|go:"GO:0051015"(actin filament binding)|go:"GO:0061003"(positive regulation of dendritic spine morphogenesis)|go:"GO:0071800"(podosome assembly)|go:"GO:0097178"(ruffle assembly)|go:"GO:0098974"(postsynaptic actin cytoskeleton organization)|interpro:IPR035717|interpro:IPR036028|mint:Q62418|reactome:R-MMU-264870|reactome:R-MMU-6794361|reactome:R-MMU-6798695|interpro:IPR001452(Src homology-3)|interpro:IPR002108(Actin-binding, cofilin/tropomyosin type)|interpro:IPR029006|interpro:IPR029923	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:uRFrfUfKF7cfhsrbleRauR33+g810090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9D0M3	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-773129|uniprotkb:Q3TDC5|uniprotkb:Q3UAN2|uniprotkb:Q63ZW4|uniprotkb:Q9DCG0|ensembl:ENSMUSP00000023210	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:cy1_mouse(display_long)|uniprotkb:Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit(gene name synonym)|uniprotkb:Cytochrome b-c1 complex subunit 4(gene name synonym)|uniprotkb:Complex III subunit 4(gene name synonym)|uniprotkb:Complex III subunit IV(gene name synonym)|uniprotkb:Cyc1(gene name)|psi-mi:Cyc1(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_079843.1|ensembl:ENSMUSG00000022551(gene)|ensembl:ENSMUST00000023210(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005750"(mitochondrial respiratory chain complex III)|go:"GO:0006122"(mitochondrial electron transport, ubiquinol to cytochrome c)|go:"GO:0008121"(ubiquinol-cytochrome-c reductase activity)|go:"GO:0020037"(heme binding)|go:"GO:0033762"(response to glucagon)|go:"GO:0046872"(metal ion binding)|interpro:IPR002326(Cytochrome c1)|interpro:IPR009056(Cytochrome c, monohaem)|interpro:IPR021157|interpro:IPR036909|mint:Q9D0M3|reactome:R-MMU-611105	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:tcTgdCV0NKIvzZsYmq8cBV4D5QY10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q60737	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-771698|uniprotkb:Q8R0X4|ensembl:ENSMUSP00000096829	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:csk21_mouse(display_long)|uniprotkb:Csnk2a1(gene name)|psi-mi:Csnk2a1(display_short)|uniprotkb:Ckiia(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_031814.2|refseq:XP_011237574.1|refseq:XP_006498720.1|dip:DIP-32409N|ensembl:ENSMUSG00000074698(gene)|ensembl:ENSMUST00000099224(transcript)|go:"GO:0000785"(chromatin)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005956"(protein kinase CK2 complex)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006915"(apoptotic process)|go:"GO:0007049"(cell cycle)|go:"GO:0008013"(beta-catenin binding)|go:"GO:0008284"(positive regulation of cell population proliferation)|go:"GO:0016055"(Wnt signaling pathway)|go:"GO:0016301"(kinase activity)|go:"GO:0016580"(Sin3 complex)|go:"GO:0016581"(NuRD complex)|go:"GO:0018105"(peptidyl-serine phosphorylation)|go:"GO:0018107"(peptidyl-threonine phosphorylation)|go:"GO:0019888"(protein phosphatase regulator activity)|go:"GO:0021987"(cerebral cortex development)|go:"GO:0030177"(positive regulation of Wnt signaling pathway)|go:"GO:0030307"(positive regulation of cell growth)|go:"GO:0033574"(response to testosterone)|go:"GO:0042802"(identical protein binding)|go:"GO:0043021"(ribonucleoprotein complex binding)|go:"GO:0043154"(negative regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0046777"(protein autophosphorylation)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048511"(rhythmic process)|go:"GO:0051726"(regulation of cell cycle)|go:"GO:0097421"(liver regeneration)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1903076"(regulation of protein localization to plasma membrane)|go:"GO:1905818"(regulation of chromosome separation)|go:"GO:2000059"(negative regulation of ubiquitin-dependent protein catabolic process)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|mint:Q60737|reactome:R-MMU-1483191|reactome:R-MMU-201688|reactome:R-MMU-2514853|reactome:R-MMU-445144|reactome:R-MMU-6804756|reactome:R-MMU-6814122|reactome:R-MMU-8934903|reactome:R-MMU-8939243|reactome:R-MMU-8948751	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:7UK+lLG3t/3xJi7CaiGSCIcxfPg10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q8BK63	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-771679|uniprotkb:Q64506|uniprotkb:Q80XI2|uniprotkb:Q99LY3|uniprotkb:Q3UIL0|ensembl:ENSMUSP00000132083	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:kc1a_mouse(display_long)|uniprotkb:CK1(gene name synonym)|uniprotkb:Csnk1a1(gene name)|psi-mi:Csnk1a1(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:XP_006526451.1|dip:DIP-32408N|ensembl:ENSMUSG00000024576(gene)|ensembl:ENSMUST00000165721(transcript)|go:"GO:0000287"(magnesium ion binding)|go:"GO:0000776"(kinetochore)|go:"GO:0000902"(cell morphogenesis)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005813"(centrosome)|go:"GO:0005829"(cytosol)|go:"GO:0005929"(cilium)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007030"(Golgi organization)|go:"GO:0007049"(cell cycle)|go:"GO:0016055"(Wnt signaling pathway)|go:"GO:0016607"(nuclear speck)|go:"GO:0018105"(peptidyl-serine phosphorylation)|go:"GO:0030877"(beta-catenin destruction complex)|go:"GO:0032436"(positive regulation of proteasomal ubiquitin-dependent protein catabolic process)|go:"GO:0036064"(ciliary basal body)|go:"GO:0042277"(peptide binding)|go:"GO:0045104"(intermediate filament cytoskeleton organization)|go:"GO:0051219"(phosphoprotein binding)|go:"GO:0051301"(cell division)|go:"GO:0090090"(negative regulation of canonical Wnt signaling pathway)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1904424"(regulation of GTP binding)|go:"GO:1990904"(ribonucleoprotein complex)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|mint:Q8BK63|reactome:R-MMU-195253|reactome:R-MMU-196299|reactome:R-MMU-4641262|reactome:R-MMU-5610785|reactome:R-MMU-5635838	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:2rG1/sMVnsRKUEWsLhLAyHvqPnY10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:O54991	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-774794|uniprotkb:A2A4K6|ensembl:ENSMUSP00000099398	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:cntp1_mouse(display_long)|uniprotkb:Neurexin-4(gene name synonym)|uniprotkb:Neurexin IV(gene name synonym)|uniprotkb:Paranodin(gene name synonym)|uniprotkb:NCP1(gene name synonym)|uniprotkb:MHDNIV(gene name synonym)|uniprotkb:Cntnap1(gene name)|psi-mi:Cntnap1(display_short)|uniprotkb:Nrxn4(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_058062.2|ensembl:ENSMUSG00000017167(gene)|ensembl:ENSMUST00000103109(transcript)|go:"GO:0002175"(protein localization to paranode region of axon)|go:"GO:0005886"(plasma membrane)|go:"GO:0005918"(septate junction)|go:"GO:0007010"(cytoskeleton organization)|go:"GO:0007155"(cell adhesion)|go:"GO:0016021"(integral component of membrane)|go:"GO:0017124"(SH3 domain binding)|go:"GO:0019227"(neuronal action potential propagation)|go:"GO:0022010"(central nervous system myelination)|go:"GO:0022011"(myelination in peripheral nervous system)|go:"GO:0030424"(axon)|go:"GO:0030913"(paranodal junction assembly)|go:"GO:0031175"(neuron projection development)|go:"GO:0033010"(paranodal junction)|go:"GO:0033270"(paranode region of axon)|go:"GO:0043209"(myelin sheath)|go:"GO:0048787"(presynaptic active zone membrane)|go:"GO:0048812"(neuron projection morphogenesis)|go:"GO:0050884"(neuromuscular process controlling posture)|go:"GO:0050885"(neuromuscular process controlling balance)|go:"GO:0071205"(protein localization to juxtaparanode region of axon)|interpro:IPR000421(Coagulation factor 5/8 type, C-terminal)|interpro:IPR000742(EGF-like, type 3)|interpro:IPR001791(Laminin G)|interpro:IPR002181(Fibrinogen, alpha/beta/gamma chain, C-terminal globular)|interpro:IPR003585(Neurexin/syndecan/glycophorin C)|interpro:IPR008979(Galactose-binding like)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR028872|interpro:IPR036056|mint:O54991	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:BTW+FjgSUq00WpWGDC6PpQcIowE10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P12960	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-774808|uniprotkb:Q6NXV7|uniprotkb:Q8BR42|uniprotkb:Q8C6A0|ensembl:ENSMUSP00000000109|ensembl:ENSMUSP00000067842|ensembl:ENSMUSP00000133063	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:cntn1_mouse(display_long)|uniprotkb:Neural cell surface protein F3(gene name synonym)|uniprotkb:Cntn1(gene name)|psi-mi:Cntn1(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001153119.1|refseq:NP_001153120.1|refseq:NP_031753.1|refseq:XP_017171911.1|ensembl:ENSMUSG00000055022(gene)|ensembl:ENSMUST00000000109(transcript)|ensembl:ENSMUST00000068378(transcript)|ensembl:ENSMUST00000169825(transcript)|go:"GO:0005886"(plasma membrane)|go:"GO:0007155"(cell adhesion)|go:"GO:0007219"(Notch signaling pathway)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0010765"(positive regulation of sodium ion transport)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0021549"(cerebellum development)|go:"GO:0030246"(carbohydrate binding)|go:"GO:0031175"(neuron projection development)|go:"GO:0043209"(myelin sheath)|go:"GO:0045121"(membrane raft)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0099025"(anchored component of postsynaptic membrane)|go:"GO:0099026"(anchored component of presynaptic membrane)|interpro:IPR003598(Immunoglobulin subtype 2)|interpro:IPR003599(Immunoglobulin subtype)|interpro:IPR003961(Fibronectin, type III)|interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013098(Immunoglobulin I-set)|interpro:IPR013151(Immunoglobulin)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR036116|interpro:IPR036179|interpro:IPR036992|mint:P12960	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:TftNvfwJlK/7DTczqiV/IzDxhOM10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q5M8N0	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-16733235|ensembl:ENSMUSP00000050036|uniprotkb:Q3TAC7|uniprotkb:Q3UFR2	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:cnrp1_mouse(display_long)|uniprotkb:Cnrip1(gene name)|psi-mi:Cnrip1(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000044629(gene)|ensembl:ENSMUST00000058159(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0005886"(plasma membrane)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0010469"(regulation of signaling receptor activity)|go:"GO:0031718"(type 1 cannabinoid receptor binding)|go:"GO:0043209"(myelin sheath)|go:"GO:2000272"(negative regulation of signaling receptor activity)|interpro:IPR029204|mint:Q5M8N0|refseq:NP_084137.1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:d4VBaXMMjEQ11X2/zohvbiwN3k010090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9DBP5	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-1635267|uniprotkb:Q8BK17|uniprotkb:Q8VD05|uniprotkb:Q9DCS7	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:kcy_mouse(display_long)|uniprotkb:Cmpk1(gene name)|psi-mi:Cmpk1(display_short)|uniprotkb:Cmk(gene name synonym)|uniprotkb:Uck(gene name synonym)|uniprotkb:Umk(gene name synonym)|uniprotkb:Umpk(gene name synonym)|uniprotkb:Cmpk(gene name synonym)|uniprotkb:Deoxycytidylate kinase(gene name synonym)|uniprotkb:Uridine monophosphate/cytidine monophosphate kinase(gene name synonym)|uniprotkb:Nucleoside-diphosphate kinase(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	interpro:IPR033690|refseq:NP_079923.3|interpro:IPR000850(Adenylate kinase)|interpro:IPR006266(UMP-CMP kinase)|interpro:IPR027417|mint:Q9DBP5|go:"GO:0004127"(cytidylate kinase activity)|go:"GO:0004550"(nucleoside diphosphate kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005730"(nucleolus)|go:"GO:0005737"(cytoplasm)|go:"GO:0006165"(nucleoside diphosphate phosphorylation)|go:"GO:0006207"('de novo' pyrimidine nucleobase biosynthetic process)|go:"GO:0006225"(UDP biosynthetic process)|go:"GO:0006227"(dUDP biosynthetic process)|go:"GO:0006240"(dCDP biosynthetic process)|go:"GO:0009041"(uridylate kinase activity)|go:"GO:0009142"(nucleoside triphosphate biosynthetic process)|go:"GO:0033862"(UMP kinase activity)|go:"GO:0036430"(CMP kinase activity)|go:"GO:0036431"(dCMP kinase activity)|go:"GO:0046705"(CDP biosynthetic process)|reactome:R-MMU-499943	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:I1hyl+mlW2kp29eULytDWnH/Fj410090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q99LI2	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-11570871|uniprotkb:A2AEK9|ensembl:ENSMUSP00000029483|ensembl:ENSMUSP00000102220	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:clcc1_mouse(display_long)|uniprotkb:Clcc1(gene name)|psi-mi:Clcc1(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:XP_017175050.1|refseq:NP_001171242.1|refseq:NP_663518.1|refseq:XP_006501479.1|ensembl:ENSMUSG00000027884(gene)|ensembl:ENSMUST00000029483(transcript)|ensembl:ENSMUST00000106609(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005254"(chloride channel activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0006821"(chloride transport)|go:"GO:0031965"(nuclear membrane)|go:"GO:0034707"(chloride channel complex)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0044233"(mitochondria-associated endoplasmic reticulum membrane)|interpro:IPR009231(Mid-1-related chloride channel)|mint:Q99LI2	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:TfbC1NQI0EaF2nSgmPacsDg+Agk10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P80316	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-772379|uniprotkb:Q3TIE0|uniprotkb:Q3U530|uniprotkb:Q3UCU4|uniprotkb:Q3UJK9|uniprotkb:Q3UWA9|uniprotkb:Q542K3|uniprotkb:Q6ZQJ1|ensembl:ENSMUSP00000022842	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:tcpe_mouse(display_long)|uniprotkb:CCT-epsilon(gene name synonym)|uniprotkb:Cct5(gene name)|psi-mi:Cct5(display_short)|uniprotkb:Ccte(gene name synonym)|uniprotkb:Kiaa0098(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_031663.1|refseq:NP_001334964.1|ensembl:ENSMUSG00000022234(gene)|ensembl:ENSMUST00000022842(transcript)|go:"GO:0003730"(mRNA 3'-UTR binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005813"(centrosome)|go:"GO:0005832"(chaperonin-containing T-complex)|go:"GO:0005874"(microtubule)|go:"GO:0006457"(protein folding)|go:"GO:0007339"(binding of sperm to zona pellucida)|go:"GO:0009615"(response to virus)|go:"GO:0031681"(G-protein beta-subunit binding)|go:"GO:0032212"(positive regulation of telomere maintenance via telomerase)|go:"GO:0043209"(myelin sheath)|go:"GO:0044183"(protein folding chaperone)|go:"GO:0044297"(cell body)|go:"GO:0048027"(mRNA 5'-UTR binding)|go:"GO:0048487"(beta-tubulin binding)|go:"GO:0050821"(protein stabilization)|go:"GO:0051082"(unfolded protein binding)|go:"GO:1901998"(toxin transport)|go:"GO:1904851"(positive regulation of establishment of protein localization to telomere)|interpro:IPR002194(Chaperonin TCP-1, conserved site)|interpro:IPR002423(Chaperonin Cpn60/TCP-1)|interpro:IPR012718(T-complex protein 1, epsilon subunit)|interpro:IPR017998|interpro:IPR027409|interpro:IPR027410|interpro:IPR027413|mint:P80316|reactome:R-MMU-390471|reactome:R-MMU-5620922|reactome:R-MMU-6814122	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:UpEEjKLyd92Zl4VnOTTlziuwAGA10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P80315	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-772370|ensembl:ENSMUSP00000133523	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:tcpd_mouse(display_long)|uniprotkb:CCT-delta(gene name synonym)|uniprotkb:A45(gene name synonym)|uniprotkb:Cct4(gene name)|psi-mi:Cct4(display_short)|uniprotkb:Cctd(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_033967.1|ensembl:ENSMUSG00000007739(gene)|ensembl:ENSMUST00000173867(transcript)|go:"GO:0002199"(zona pellucida receptor complex)|go:"GO:0005524"(ATP binding)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005813"(centrosome)|go:"GO:0005829"(cytosol)|go:"GO:0005832"(chaperonin-containing T-complex)|go:"GO:0005874"(microtubule)|go:"GO:0005929"(cilium)|go:"GO:0006457"(protein folding)|go:"GO:0007339"(binding of sperm to zona pellucida)|go:"GO:0032212"(positive regulation of telomere maintenance via telomerase)|go:"GO:0042470"(melanosome)|go:"GO:0044183"(protein folding chaperone)|go:"GO:0044297"(cell body)|go:"GO:0050821"(protein stabilization)|go:"GO:0051082"(unfolded protein binding)|go:"GO:0051973"(positive regulation of telomerase activity)|go:"GO:0090666"(scaRNA localization to Cajal body)|go:"GO:1901998"(toxin transport)|go:"GO:1904851"(positive regulation of establishment of protein localization to telomere)|go:"GO:1904874"(positive regulation of telomerase RNA localization to Cajal body)|interpro:IPR002194(Chaperonin TCP-1, conserved site)|interpro:IPR002423(Chaperonin Cpn60/TCP-1)|interpro:IPR012717(T-complex protein 1, delta subunit)|interpro:IPR017998|interpro:IPR027409|interpro:IPR027410|interpro:IPR027413|mint:P80315|reactome:R-MMU-390471|reactome:R-MMU-5620922|reactome:R-MMU-6814122	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:/40BY5vhE/fuloWu9L2k7ujy9Nc10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q8VDP4	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-2931660|ensembl:ENSMUSP00000036924|uniprotkb:Q6PIB1|uniprotkb:Q8BWR5|uniprotkb:Q8C0F0|uniprotkb:Q8R3G6	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:ccar2_mouse(display_long)|uniprotkb:Ccar2(gene name)|psi-mi:Ccar2(display_short)|uniprotkb:Cell division cycle and apoptosis regulator protein 2(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000033712(gene)|ensembl:ENSMUST00000035612(transcript)|go:"GO:0000785"(chromatin)|go:"GO:0000993"(RNA polymerase II complex binding)|go:"GO:0004857"(enzyme inhibitor activity)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005759"(mitochondrial matrix)|go:"GO:0005819"(spindle)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0006397"(mRNA processing)|go:"GO:0006974"(cellular response to DNA damage stimulus)|go:"GO:0007049"(cell cycle)|go:"GO:0008380"(RNA splicing)|go:"GO:0009411"(response to UV)|go:"GO:0016055"(Wnt signaling pathway)|go:"GO:0019899"(enzyme binding)|go:"GO:0030308"(negative regulation of cell growth)|go:"GO:0030374"(nuclear receptor coactivator activity)|go:"GO:0031647"(regulation of protein stability)|go:"GO:0032435"(negative regulation of proteasomal ubiquitin-dependent protein catabolic process)|go:"GO:0032784"(regulation of DNA-templated transcription, elongation)|go:"GO:0042752"(regulation of circadian rhythm)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043086"(negative regulation of catalytic activity)|go:"GO:0043653"(mitochondrial fragmentation involved in apoptotic process)|go:"GO:0044609"(DBIRD complex)|go:"GO:0045892"(negative regulation of transcription, DNA-templated)|go:"GO:0048511"(rhythmic process)|go:"GO:0090263"(positive regulation of canonical Wnt signaling pathway)|go:"GO:0090311"(regulation of protein deacetylation)|go:"GO:1902230"(negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage)|go:"GO:2000003"(positive regulation of DNA damage checkpoint)|interpro:IPR011992(EF-Hand type)|interpro:IPR025223|interpro:IPR025224|interpro:IPR025954|interpro:IPR028811|mint:Q8VDP4|reactome:R-MMU-3371453|refseq:NP_666167.3|refseq:XP_006518959.1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:4KqmxaC2o3+yWOF9dwQ9kQ7AMqs10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q923T9	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-771638|uniprotkb:Q3U3H3|uniprotkb:Q8VED3|ensembl:ENSMUSP00000071720	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:kcc2g_mouse(display_long)|uniprotkb:Camk2g(gene name)|psi-mi:Camk2g(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001034227.1|refseq:NP_001034228.1|refseq:NP_848712.2|ensembl:ENSMUSG00000021820(gene)|ensembl:ENSMUST00000071816(transcript)|go:"GO:0000082"(G1/S transition of mitotic cell cycle)|go:"GO:0001666"(response to hypoxia)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004683"(calmodulin-dependent protein kinase activity)|go:"GO:0005516"(calmodulin binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005954"(calcium- and calmodulin-dependent protein kinase complex)|go:"GO:0006816"(calcium ion transport)|go:"GO:0006979"(response to oxidative stress)|go:"GO:0007399"(nervous system development)|go:"GO:0010975"(regulation of neuron projection development)|go:"GO:0014069"(postsynaptic density)|go:"GO:0030154"(cell differentiation)|go:"GO:0033017"(sarcoplasmic reticulum membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0043005"(neuron projection)|go:"GO:0046777"(protein autophosphorylation)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1901897"(regulation of relaxation of cardiac muscle)|go:"GO:1903076"(regulation of protein localization to plasma membrane)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR013543(Calcium/calmodulin dependent protein kinase II, association-domain)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR032710|mint:Q923T9|reactome:R-MMU-3371571|reactome:R-MMU-399719|reactome:R-MMU-438066|reactome:R-MMU-5576892|reactome:R-MMU-5578775|reactome:R-MMU-5673000|reactome:R-MMU-5673001|reactome:R-MMU-877300|reactome:R-MMU-936837	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:pbJqcEUQJQRnAwcKozmS72n9s1w10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q02105	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-20567414|uniprotkb:Q6DI63|ensembl:ENSMUSP00000036747	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:c1qc_mouse(display_long)|uniprotkb:C1qc(gene name)|psi-mi:C1qc(display_short)|uniprotkb:C1qg(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_031600.2|refseq:XP_017175416.1|ensembl:ENSMUSG00000036896(gene)|ensembl:ENSMUST00000046332(transcript)|go:"GO:0005581"(collagen trimer)|go:"GO:0005615"(extracellular space)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0030853"(negative regulation of granulocyte differentiation)|go:"GO:0045087"(innate immune response)|go:"GO:0045202"(synapse)|go:"GO:0045650"(negative regulation of macrophage differentiation)|go:"GO:0098794"(postsynapse)|go:"GO:0098883"(synapse pruning)|interpro:IPR001073(Complement C1q protein)|interpro:IPR008160(Collagen triple helix repeat)|interpro:IPR008983(Tumour necrosis factor-like)|mint:Q02105|reactome:R-MMU-166663|reactome:R-MMU-173623|reactome:R-MMU-977606	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:bS60z9qFSICByYWP9YbySA/E2dM10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P14106	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-2794395|uniprotkb:Q3U793|ensembl:ENSMUSP00000040246	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:c1qb_mouse(display_long)|uniprotkb:C1qb(gene name)|psi-mi:C1qb(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_033907.1|ensembl:ENSMUSG00000036905(gene)|ensembl:ENSMUST00000046384(transcript)|go:"GO:0005581"(collagen trimer)|go:"GO:0005602"(complement component C1 complex)|go:"GO:0005615"(extracellular space)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0042802"(identical protein binding)|go:"GO:0045087"(innate immune response)|go:"GO:0045202"(synapse)|go:"GO:0048839"(inner ear development)|go:"GO:0098794"(postsynapse)|go:"GO:0098883"(synapse pruning)|interpro:IPR001073(Complement C1q protein)|interpro:IPR008160(Collagen triple helix repeat)|interpro:IPR008983(Tumour necrosis factor-like)|interpro:IPR037573|mint:P14106|reactome:R-MMU-166663|reactome:R-MMU-173623|reactome:R-MMU-977606	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:foGutZBoR+VSolYUxHhWd55XgOo10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P98086	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-299840|ensembl:ENSMUSP00000048836|intact:EBI-6171723|uniprotkb:Q9DCM6	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:c1qa_mouse(display_long)|uniprotkb:C1qa(gene name)|psi-mi:C1qa(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000036887(gene)|interpro:IPR001073(Complement C1q protein)|interpro:IPR008160(Collagen triple helix repeat)|interpro:IPR008983(Tumour necrosis factor-like)|ensembl:ENSMUST00000046285(transcript)|go:"GO:0001774"(microglial cell activation)|go:"GO:0005581"(collagen trimer)|go:"GO:0005615"(extracellular space)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0007568"(aging)|go:"GO:0010039"(response to iron ion)|go:"GO:0016322"(neuron remodeling)|go:"GO:0045087"(innate immune response)|go:"GO:0045202"(synapse)|go:"GO:0048143"(astrocyte activation)|go:"GO:0050808"(synapse organization)|go:"GO:0098794"(postsynapse)|go:"GO:0098883"(synapse pruning)|go:"GO:0150062"(complement-mediated synapse pruning)|go:"GO:0150064"(vertebrate eye-specific patterning)|go:"GO:1901216"(positive regulation of neuron death)|interpro:IPR037572|mint:P98086|reactome:R-MMU-166663|reactome:R-MMU-173623|reactome:R-MMU-977606|refseq:NP_031598.2	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:QjjOX9mLtiqcbgHsEOww/qvEdH410090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:O88737	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-400493|uniprotkb:Q6ZQB5|uniprotkb:E9QMZ3|ensembl:ENSMUSP00000035208	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:bsn_mouse(display_long)|uniprotkb:Bsn(gene name)|psi-mi:Bsn(display_short)|uniprotkb:Kiaa0434(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_031593.2|go:"GO:0008021"(synaptic vesicle)|go:"GO:0008088"(axo-dendritic transport)|go:"GO:0008090"(retrograde axonal transport)|go:"GO:0009986"(cell surface)|go:"GO:0014069"(postsynaptic density)|go:"GO:0019898"(extrinsic component of membrane)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0035418"(protein localization to synapse)|go:"GO:0043025"(neuronal cell body)|go:"GO:0044306"(neuron projection terminus)|go:"GO:0045202"(synapse)|go:"GO:0045503"(dynein light chain binding)|go:"GO:0046872"(metal ion binding)|go:"GO:0048786"(presynaptic active zone)|go:"GO:0048788"(cytoskeleton of presynaptic active zone)|go:"GO:0050808"(synapse organization)|go:"GO:0060076"(excitatory synapse)|go:"GO:0060077"(inhibitory synapse)|go:"GO:0097470"(ribbon synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098693"(regulation of synaptic vesicle cycle)|go:"GO:0098793"(presynapse)|go:"GO:0098831"(presynaptic active zone cytoplasmic component)|go:"GO:0098882"(structural constituent of presynaptic active zone)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099526"(presynapse to nucleus signaling pathway)|go:"GO:1904071"(presynaptic active zone assembly)|go:"GO:1904115"(axon cytoplasm)|go:"GO:1904666"(regulation of ubiquitin protein ligase activity)|go:"GO:1990257"(piccolo-bassoon transport vesicle)|interpro:IPR008899(Zinc finger, piccolo-type)|interpro:IPR011011(Zinc finger, FYVE/PHD-type)|interpro:IPR013083(Zinc finger, RING/FYVE/PHD-type)|interpro:IPR030627|mint:O88737|ensembl:ENSMUSG00000032589(gene)|ensembl:ENSMUST00000035208(transcript)|go:"GO:0001222"(transcription corepressor binding)|go:"GO:0005798"(Golgi-associated vesicle)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|refseq:XP_006511697.1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:W4w0sRW518SHtgLQ/mY/unJDgwc10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9QWY8	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-698498|uniprotkb:Q99LV8|uniprotkb:Q9Z2B6|uniprotkb:O08612|uniprotkb:Q80TG8|uniprotkb:Q80UV6|uniprotkb:Q505F0|ensembl:ENSMUSP00000134825	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:asap1_mouse(display_long)|uniprotkb:PIP2-dependent ARF1 GAP(gene name synonym)|uniprotkb:ADP-ribosylation factor-directed GTPase-activating protein 1(gene name synonym)|uniprotkb:Development and differentiation-enhancing factor 1(gene name synonym)|uniprotkb:Asap1(gene name)|psi-mi:Asap1(display_short)|uniprotkb:Ddef1(gene name synonym)|uniprotkb:Kiaa1249(gene name synonym)|uniprotkb:Shag1(gene name synonym)|uniprotkb:130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001263396.1|refseq:NP_034156.2|refseq:NP_001263390.1|refseq:NP_001263391.1|ensembl:ENSMUSG00000022377(gene)|ensembl:ENSMUST00000177374(transcript)|go:"GO:0001786"(phosphatidylserine binding)|go:"GO:0002102"(podosome)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005546"(phosphatidylinositol-4,5-bisphosphate binding)|go:"GO:0005547"(phosphatidylinositol-3,4,5-trisphosphate binding)|go:"GO:0005829"(cytosol)|go:"GO:0031253"(cell projection membrane)|go:"GO:0043197"(dendritic spine)|go:"GO:0043547"(positive regulation of GTPase activity)|go:"GO:0046872"(metal ion binding)|go:"GO:0060271"(cilium assembly)|go:"GO:0061000"(negative regulation of dendritic spine development)|go:"GO:0071803"(positive regulation of podosome assembly)|go:"GO:1903527"(positive regulation of membrane tubulation)|interpro:IPR001164(Arf GTPase activating protein)|interpro:IPR001452(Src homology-3)|interpro:IPR001849(Pleckstrin-like)|interpro:IPR002110(Ankyrin)|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR020683|interpro:IPR027267|interpro:IPR036028|interpro:IPR036770|interpro:IPR037278|interpro:IPR037844|interpro:IPR037928|interpro:IPR038016|interpro:IPR038508|interpro:IPR043593|mint:Q9QWY8|rcsb pdb:5C6R|rcsb pdb:5C79|reactome:R-MMU-5620916	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:/jhMt+/t+DTqI3ZQmzU0IMFCHdA10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9ES28	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-642580|uniprotkb:O08757|uniprotkb:Q3UTS5|uniprotkb:Q6XPA5|uniprotkb:Q6ZQI5|uniprotkb:Q8C750|uniprotkb:Q91ZZ6|uniprotkb:Q9ES27	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:arhg7_mouse(display_long)|uniprotkb:PAK-interacting exchange factor beta(gene name synonym)|uniprotkb:Beta-Pix(gene name synonym)|uniprotkb:p85SPR(gene name synonym)|uniprotkb:Arhgef7(gene name)|psi-mi:Arhgef7(display_short)|uniprotkb:Pak3bp(gene name synonym)|uniprotkb:Kiaa0142(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001106989.1|refseq:NP_001106990.1|refseq:NP_059098.2|interpro:IPR001715(Calponin-like actin-binding)|interpro:IPR000219("Dbl homology (DH) domain")|interpro:IPR001331(Guanine-nucleotide dissociation stimulator, CDC24, conserved site)|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR001849(Pleckstrin-like)|interpro:IPR001452(Src homology-3)|refseq:XP_006508906.2|refseq:XP_006508907.2|interpro:IPR032409|refseq:XP_006508904.2|reactome:R-MMU-416482|reactome:R-MMU-8980692|reactome:R-MMU-9013148|reactome:R-MMU-9013149|reactome:R-MMU-9013406|reactome:R-MMU-9013420|reactome:R-MMU-9013424|go:"GO:0000322"(storage vacuole)|go:"GO:0001726"(ruffle)|go:"GO:0002244"(hematopoietic progenitor cell differentiation)|go:"GO:0005085"(guanyl-nucleotide exchange factor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005813"(centrosome)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0005938"(cell cortex)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0019901"(protein kinase binding)|go:"GO:0030027"(lamellipodium)|go:"GO:0030032"(lamellipodium assembly)|go:"GO:0030426"(growth cone)|go:"GO:0032092"(positive regulation of protein binding)|go:"GO:0032991"(protein-containing complex)|go:"GO:0043005"(neuron projection)|go:"GO:0043015"(gamma-tubulin binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043547"(positive regulation of GTPase activity)|go:"GO:0043615"(astrocyte cell migration)|go:"GO:0060124"(positive regulation of growth hormone secretion)|go:"GO:0097431"(mitotic spindle pole)|go:"GO:0098794"(postsynapse)|go:"GO:0098974"(postsynaptic actin cytoskeleton organization)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099140"(presynaptic actin cytoskeleton organization)|go:"GO:1904424"(regulation of GTP binding)|go:"GO:1905833"(negative regulation of microtubule nucleation)|rcsb pdb:2ESW|rcsb pdb:6JMT|reactome:R-MMU-182971|reactome:R-MMU-193648|reactome:R-MMU-3928664|mint:Q9ES28|interpro:IPR035789|interpro:IPR035899|interpro:IPR036028|interpro:IPR036872	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:+Bie+UoaAI9/ldibUxAjNYc3sYs10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q8BW86	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-26595823|ensembl:ENSMUSP00000063284|ensembl:ENSMUSP00000153224	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:arg33_mouse(display_long)|uniprotkb:Arhgef33(gene name)|psi-mi:Arhgef33(display_short)|uniprotkb:Gm941(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000054901(gene)|ensembl:ENSMUST00000068175(transcript)|ensembl:ENSMUST00000223878(transcript)|go:"GO:0005085"(guanyl-nucleotide exchange factor activity)|interpro:IPR000219("Dbl homology (DH) domain")|interpro:IPR035899|interpro:IPR042849|mint:Q8BW86|reactome:R-MMU-193648|reactome:R-MMU-416482|refseq:NP_001138924.1|refseq:XP_006524586.1|refseq:XP_006524587.1|refseq:XP_017173034.1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:kt1xQR1EkSToFg+TI822cEISJXY10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q8R2R9	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-775262|uniprotkb:Q3UYJ3|uniprotkb:Q923G7|ensembl:ENSMUSP00000128446|ensembl:ENSMUSP00000147967	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:ap3m2_mouse(display_long)|uniprotkb:Mu3B-adaptin(gene name synonym)|uniprotkb:Golgi adaptor AP-1 47 kDa protein homolog 2(gene name synonym)|uniprotkb:HA1 47 kDa subunit homolog 2(gene name synonym)|uniprotkb:Clathrin coat assembly protein AP47 homolog 2(gene name synonym)|uniprotkb:Clathrin coat-associated protein AP47 homolog 2(gene name synonym)|uniprotkb:P47B(gene name synonym)|uniprotkb:Ap3m2(gene name)|psi-mi:Ap3m2(display_short)|uniprotkb:Clathrin assembly protein assembly protein complex 3 mu-2 medium chain(gene name synonym)|uniprotkb:Adaptor-related protein complex 3 subunit mu-2(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001116292.1|refseq:NP_083781.2|ensembl:ENSMUSG00000031539(gene)|ensembl:ENSMUST00000163739(transcript)|ensembl:ENSMUST00000210656(transcript)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006897"(endocytosis)|go:"GO:0008089"(anterograde axonal transport)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0030131"(clathrin adaptor complex)|go:"GO:0030659"(cytoplasmic vesicle membrane)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0048490"(anterograde synaptic vesicle transport)|go:"GO:1904115"(axon cytoplasm)|interpro:IPR001392(Clathrin adaptor, mu subunit)|interpro:IPR011012(Longin-like)|interpro:IPR018240|interpro:IPR022775|interpro:IPR028565|interpro:IPR036168|mint:Q8R2R9	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:oowb5lTzk6pT2nDM3jgNAUWqDmM10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P62743	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-1033702|uniprotkb:P70626|uniprotkb:P97626|uniprotkb:Q00380|ensembl:ENSMUSP00000083281	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:ap2s1_mouse(display_long)|uniprotkb:Ap2s1(gene name)|psi-mi:Ap2s1(display_short)|uniprotkb:Ap17(gene name synonym)|uniprotkb:Claps2(gene name synonym)|uniprotkb:Sigma2-adaptin(gene name synonym)|uniprotkb:Sigma-adaptin 3b(gene name synonym)|uniprotkb:Plasma membrane adaptor AP-2 17 kDa protein(gene name synonym)|uniprotkb:Clathrin coat assembly protein AP17(gene name synonym)|uniprotkb:Clathrin coat-associated protein AP17(gene name synonym)|uniprotkb:Adaptor protein complex AP-2 subunit sigma(gene name synonym)|uniprotkb:Clathrin assembly protein 2 sigma small chain(gene name synonym)|uniprotkb:Adaptor-related protein complex 2 subunit sigma(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000008036(gene)|ensembl:ENSMUST00000086112(transcript)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0030122"(AP-2 adaptor complex)|go:"GO:0035615"(clathrin adaptor activity)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0072583"(clathrin-dependent endocytosis)|interpro:IPR000804(Clathrin adaptor, sigma subunit/coatomer, zeta subunit)|interpro:IPR011012(Longin-like)|interpro:IPR016635(Adaptor protein complex, sigma subunit)|interpro:IPR022775|interpro:IPR027156|mint:P62743|rcsb pdb:2JKR|rcsb pdb:2JKT|rcsb pdb:2VGL|rcsb pdb:2XA7|rcsb pdb:4UQI|rcsb pdb:6QH5|rcsb pdb:6QH6|rcsb pdb:6QH7|rcsb pdb:6YAE|rcsb pdb:6YAF|rcsb pdb:6YAH|reactome:R-MMU-177504|reactome:R-MMU-2132295|reactome:R-MMU-416993|reactome:R-MMU-437239|reactome:R-MMU-5099900|reactome:R-MMU-5140745|reactome:R-MMU-8856825|reactome:R-MMU-8856828|reactome:R-MMU-8866427|reactome:R-MMU-8964038|refseq:NP_941015.2|dip:DIP-37505N	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:AJxHeeoCpwPxjsRcAQkiAGaC9k010090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P17427	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-775215|uniprotkb:Q8C2J5|uniprotkb:Q921V0|ensembl:ENSMUSP00000003038	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:ap2a2_mouse(display_long)|uniprotkb:Adaptor protein complex AP-2 subunit alpha-2(gene name synonym)|uniprotkb:Alpha2-adaptin(gene name synonym)|uniprotkb:Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit(gene name synonym)|uniprotkb:Alpha-adaptin C(gene name synonym)|uniprotkb:Clathrin assembly protein complex 2 alpha-C large chain(gene name synonym)|uniprotkb:100 kDa coated vesicle protein C(gene name synonym)|uniprotkb:Ap2a2(gene name)|psi-mi:Ap2a2(display_short)|uniprotkb:Adtab(gene name synonym)|uniprotkb:Adaptor-related protein complex 2 subunit alpha-2(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_031485.3|dip:DIP-32160N|ensembl:ENSMUSG00000002957(gene)|ensembl:ENSMUST00000003038(transcript)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019900"(kinase binding)|go:"GO:0019901"(protein kinase binding)|go:"GO:0019904"(protein domain specific binding)|go:"GO:0030117"(membrane coat)|go:"GO:0030122"(AP-2 adaptor complex)|go:"GO:0030141"(secretory granule)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0035091"(phosphatidylinositol binding)|go:"GO:0035615"(clathrin adaptor activity)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0072583"(clathrin-dependent endocytosis)|go:"GO:0097718"(disordered domain specific binding)|go:"GO:0140312"(cargo adaptor activity)|go:"GO:1902036"(regulation of hematopoietic stem cell differentiation)|interpro:IPR002553(Clathrin/coatomer adaptor, adaptin-like, N-terminal)|interpro:IPR003164(Clathrin adaptor, alpha-adaptin, appendage, C-terminal subdomain)|interpro:IPR008152(Clathrin adaptor, alpha/beta/gamma-adaptin, appendage, Ig-like subdomain)|interpro:IPR009028(Clathrin/coatomer adaptor, adaptin-like, appendage, C-terminal subdomain)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR012295(Beta2-adaptin/TATA-box binding, C-terminal)|interpro:IPR013041(Clathrin/coatomer adaptor, adaptin-like, appendage, Ig-like subdomain)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR017104(Adaptor protein complex AP-2, alpha subunit)|mint:P17427|rcsb pdb:1B9K|rcsb pdb:1KY6|rcsb pdb:1KY7|rcsb pdb:1KYD|rcsb pdb:1KYF|rcsb pdb:1KYU|rcsb pdb:1QTP|rcsb pdb:1QTS|rcsb pdb:1W80|rcsb pdb:2JKR|rcsb pdb:2JKT|rcsb pdb:2VJ0|rcsb pdb:3HS8|rcsb pdb:6OWO|rcsb pdb:6OXL|reactome:R-MMU-177504|reactome:R-MMU-2132295|reactome:R-MMU-416993|reactome:R-MMU-437239|reactome:R-MMU-5099900|reactome:R-MMU-5140745|reactome:R-MMU-6798695|reactome:R-MMU-8856825|reactome:R-MMU-8856828|reactome:R-MMU-8866427|reactome:R-MMU-8964038	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:KlOjm5d8qxxQkcuD2Hukoj8DLss10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9R0Y5	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-777466|uniprotkb:A2AK80|uniprotkb:Q542C5|uniprotkb:Q9R0Y4|ensembl:ENSMUSP00000108902|ensembl:ENSMUSP00000108903	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:kad1_mouse(display_long)|uniprotkb:ATP-AMP transphosphorylase 1(gene name synonym)|uniprotkb:Myokinase(gene name synonym)|uniprotkb:Ak1(gene name)|psi-mi:Ak1(display_short)|uniprotkb:"ATP:AMP phosphotransferase"(gene name synonym)|uniprotkb:Adenylate monophosphate kinase(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001185719.1|refseq:NP_001185720.1|refseq:NP_001185721.1|refseq:NP_067490.1|refseq:XP_006497687.1|ensembl:ENSMUSG00000026817(gene)|ensembl:ENSMUST00000113277(transcript)|ensembl:ENSMUST00000113278(transcript)|go:"GO:0001520"(outer dense fiber)|go:"GO:0004017"(adenylate kinase activity)|go:"GO:0004550"(nucleoside diphosphate kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006165"(nucleoside diphosphate phosphorylation)|go:"GO:0006172"(ADP biosynthetic process)|go:"GO:0009142"(nucleoside triphosphate biosynthetic process)|go:"GO:0010828"(positive regulation of glucose transmembrane transport)|go:"GO:0030017"(sarcomere)|go:"GO:0036126"(sperm flagellum)|go:"GO:0043005"(neuron projection)|go:"GO:0046033"(AMP metabolic process)|go:"GO:0046034"(ATP metabolic process)|go:"GO:0046103"(inosine biosynthetic process)|go:"GO:0048471"(perinuclear region of cytoplasm)|interpro:IPR000850(Adenylate kinase)|interpro:IPR006267(Adenylate kinase, isozyme 1)|interpro:IPR027417|interpro:IPR028582|interpro:IPR033690|mint:Q9R0Y5|reactome:R-MMU-499943	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:HTydMeIFNEaVZDs7sEN7aLieuVg10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P54923	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-26595804|ensembl:ENSMUSP00000002923	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:adprh_mouse(display_long)|uniprotkb:ADP-ribose-L-arginine cleaving enzyme(gene name synonym)|uniprotkb:Adprh(gene name)|psi-mi:Adprh(display_short)|uniprotkb:Arh1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000002844(gene)|ensembl:ENSMUST00000002923(transcript)|go:"GO:0000287"(magnesium ion binding)|go:"GO:0003875"(ADP-ribosylarginine hydrolase activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0030955"(potassium ion binding)|go:"GO:0051725"(protein de-ADP-ribosylation)|interpro:IPR005502(ADP-ribosylation/Crystallin J1)|interpro:IPR012108(ADP-ribosylarginine hydrolase)|interpro:IPR036705|mint:P54923|refseq:NP_031440.1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:bRgQFMpZzE3Ildc19EzZvk8kz8U10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q641P0	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-26471986|ensembl:ENSMUSP00000085578|uniprotkb:Q7TSH1	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:arp3b_mouse(display_long)|uniprotkb:Actr3b(gene name)|psi-mi:Actr3b(display_short)|uniprotkb:ARP3-beta(gene name synonym)|uniprotkb:Actin-like protein 3B(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000056367(gene)|ensembl:ENSMUST00000088244(transcript)|go:"GO:0003779"(actin binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005856"(cytoskeleton)|go:"GO:0042995"(cell projection)|interpro:IPR004000(Actin/actin-like)|interpro:IPR020902|interpro:IPR043129|mint:Q641P0|refseq:NP_001004365.1|refseq:NP_001297433.1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:Dgvqzk0jTsysP2LG9zi2YYMNax410090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P61164	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-774242|uniprotkb:P42024|uniprotkb:Q3TJF9|ensembl:ENSMUSP00000039844	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:actz_mouse(display_long)|uniprotkb:Centrosome-associated actin homolog(gene name synonym)|uniprotkb:Actin-RPV(gene name synonym)|uniprotkb:ARP1(gene name synonym)|uniprotkb:Actr1a(gene name)|psi-mi:Actr1a(display_short)|uniprotkb:Ctrn1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_058556.1|ensembl:ENSMUSG00000025228(gene)|ensembl:ENSMUST00000040270(transcript)|go:"GO:0002177"(manchette)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005813"(centrosome)|go:"GO:0005814"(centriole)|go:"GO:0007283"(spermatogenesis)|go:"GO:0015630"(microtubule cytoskeleton)|go:"GO:0030137"(COPI-coated vesicle)|go:"GO:0043209"(myelin sheath)|go:"GO:0099738"(cell cortex region)|interpro:IPR004000(Actin/actin-like)|interpro:IPR004001(Actin, conserved site)|interpro:IPR020902|interpro:IPR043129|mint:P61164|reactome:R-MMU-2132295|reactome:R-MMU-2565942|reactome:R-MMU-3371497|reactome:R-MMU-380259|reactome:R-MMU-380270|reactome:R-MMU-380284|reactome:R-MMU-380320|reactome:R-MMU-5620912|reactome:R-MMU-6807878|reactome:R-MMU-6811436|reactome:R-MMU-8854518	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:aJHKOSj1qmZ7Pk8aZAyWWd8DEdw10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q3UHJ0	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-771618|uniprotkb:B2RUJ0|uniprotkb:Q3TY53|uniprotkb:Q6ZPZ6|uniprotkb:Q80XP6|ensembl:ENSMUSP00000086948	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:aak1_mouse(display_long)|uniprotkb:Adaptor-associated kinase 1(gene name synonym)|uniprotkb:Aak1(gene name)|psi-mi:Aak1(display_short)|uniprotkb:Kiaa1048(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001035195.1|refseq:NP_808430.2|go:"GO:0005737"(cytoplasm)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006897"(endocytosis)|go:"GO:0019897"(extrinsic component of plasma membrane)|go:"GO:0030136"(clathrin-coated vesicle)|ensembl:ENSMUSG00000057230(gene)|ensembl:ENSMUST00000089519(transcript)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0005112"(Notch binding)|go:"GO:0005524"(ATP binding)|go:"GO:0031252"(cell leading edge)|go:"GO:0032880"(regulation of protein localization)|go:"GO:0035612"(AP-2 adaptor complex binding)|go:"GO:0043195"(terminal bouton)|go:"GO:0045747"(positive regulation of Notch signaling pathway)|go:"GO:0046777"(protein autophosphorylation)|go:"GO:0050821"(protein stabilization)|go:"GO:0071439"(clathrin complex)|go:"GO:0098793"(presynapse)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:2000369"(regulation of clathrin-dependent endocytosis)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|mint:Q3UHJ0|reactome:R-MMU-8856825|reactome:R-MMU-8856828	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:VdUCkCmofdJSa676n0jLS8VLDso10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q8CBE3	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-912188|uniprotkb:Q80Y96|uniprotkb:Q8CCL2|ensembl:ENSMUSP00000021572|ensembl:ENSMUSP00000062174	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:wdr37_mouse(display_long)|uniprotkb:Wdr37(gene name)|psi-mi:Wdr37(display_short)|uniprotkb:Kiaa0982(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001034477.1|refseq:NP_766033.1|refseq:XP_006516532.1|refseq:XP_006516533.1|ensembl:ENSMUSG00000021147(gene)|ensembl:ENSMUST00000021572(transcript)|ensembl:ENSMUST00000054251(transcript)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0030687"(preribosome, large subunit precursor)|interpro:IPR001680(WD40 repeat)|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR019775|interpro:IPR020472|interpro:IPR036322|mint:Q8CBE3	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:lwhck22UFkJCcpVKLEA90ajEBqc10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q8R5H6	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-774719|uniprotkb:Q91W51|uniprotkb:Q9ERQ9|ensembl:ENSMUSP00000019975|ensembl:ENSMUSP00000101148	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:wasf1_mouse(display_long)|uniprotkb:Protein WAVE-1(gene name synonym)|uniprotkb:Wasf1(gene name)|psi-mi:Wasf1(display_short)|uniprotkb:Wave1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_114083.1|refseq:XP_006512995.1|ensembl:ENSMUSG00000019831(gene)|ensembl:ENSMUST00000019975(transcript)|ensembl:ENSMUST00000105509(transcript)|go:"GO:0003779"(actin binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005856"(cytoskeleton)|go:"GO:0005925"(focal adhesion)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0016601"(Rac protein signal transduction)|go:"GO:0030027"(lamellipodium)|go:"GO:0030036"(actin cytoskeleton organization)|go:"GO:0031175"(neuron projection development)|go:"GO:0031209"(SCAR complex)|go:"GO:0031267"(small GTPase binding)|go:"GO:0032839"(dendrite cytoplasm)|go:"GO:0032991"(protein-containing complex)|go:"GO:0034237"(protein kinase A regulatory subunit binding)|go:"GO:0051018"(protein kinase A binding)|go:"GO:0051388"(positive regulation of neurotrophin TRK receptor signaling pathway)|go:"GO:0070584"(mitochondrion morphogenesis)|go:"GO:0071933"(Arp2/3 complex binding)|go:"GO:0072673"(lamellipodium morphogenesis)|go:"GO:0097484"(dendrite extension)|go:"GO:0098794"(postsynapse)|go:"GO:0098885"(modification of postsynaptic actin cytoskeleton)|go:"GO:0098939"(dendritic transport of mitochondrion)|go:"GO:1990416"(cellular response to brain-derived neurotrophic factor stimulus)|go:"GO:2000601"(positive regulation of Arp2/3 complex-mediated actin nucleation)|interpro:IPR003124(Actin-binding WH2)|interpro:IPR028288|mint:Q8R5H6|reactome:R-MMU-2029482|reactome:R-MMU-4420097|reactome:R-MMU-5663213|reactome:R-MMU-9013149|reactome:R-MMU-9013423	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:t+4F2ap7lNjE/AGuIwMhel4YknU10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q8CC88	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-12602574|ensembl:ENSMUSP00000048925|uniprotkb:Q3U4P9|uniprotkb:Q6ZQ94|uniprotkb:Q8CDH8|uniprotkb:Q8VCU3	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:vwa8_mouse(display_long)|uniprotkb:Vwa8(gene name)|psi-mi:Vwa8(display_short)|uniprotkb:Kiaa0564(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000058997(gene)|ensembl:ENSMUST00000040990(transcript)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005777"(peroxisome)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|interpro:IPR002035(von Willebrand factor, type A)|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR011704(ATPase associated with various cellular activities, AAA-5)|interpro:IPR027417|interpro:IPR036465|interpro:IPR039891|mint:Q8CC88|refseq:NP_082182.1|refseq:NP_776119.2	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:JRlZvEqyEowI+W/4vQZy0mZEQk810090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q62059	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-772574|uniprotkb:Q62058|uniprotkb:Q9CUU0	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:cspg2_mouse(display_long)|uniprotkb:Chondroitin sulfate proteoglycan core protein 2(gene name synonym)|uniprotkb:PG-M(gene name synonym)|uniprotkb:Cspg2(gene name synonym)|uniprotkb:Large fibroblast proteoglycan(gene name synonym)|uniprotkb:Vcan(gene name)|psi-mi:Vcan(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	interpro:IPR001304(C-type lectin)|interpro:IPR016186(C-type lectin-like)|interpro:IPR018378|interpro:IPR016187(C-type lectin fold)|interpro:IPR001881(EGF-like calcium-binding)|interpro:IPR000152(Aspartic acid and asparagine hydroxylation site)|interpro:IPR000742(EGF-like, type 3)|interpro:IPR018097|interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003599(Immunoglobulin subtype)|interpro:IPR013106(Immunoglobulin V-set)|interpro:IPR000538(Link)|interpro:IPR000436(Sushi/SCR/CCP)|interpro:IPR033987|mint:Q62059|interpro:IPR035976|interpro:IPR036179|go:"GO:0007417"(central nervous system development)|go:"GO:0007507"(heart development)|go:"GO:0008347"(glial cell migration)|go:"GO:0009986"(cell surface)|go:"GO:0019903"(protein phosphatase binding)|go:"GO:0030246"(carbohydrate binding)|go:"GO:0031012"(extracellular matrix)|go:"GO:0033165"(interphotoreceptor matrix)|go:"GO:0043666"(regulation of phosphoprotein phosphatase activity)|go:"GO:0045202"(synapse)|go:"GO:0062023"(collagen-containing extracellular matrix)|go:"GO:0072534"(perineuronal net)|reactome:R-MMU-1971475|reactome:R-MMU-2022870|reactome:R-MMU-2022923|reactome:R-MMU-2024101|reactome:R-MMU-3000178|reactome:R-MMU-381426|reactome:R-MMU-8957275|go:"GO:0001501"(skeletal system development)|go:"GO:0001657"(ureteric bud development)|go:"GO:0001750"(photoreceptor outer segment)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005540"(hyaluronic acid binding)|go:"GO:0005576"(extracellular region)|go:"GO:0007155"(cell adhesion)	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:pDGK/Hj7x5nFPw16KL9ROydQyno10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q8R1I1	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-1219641|ensembl:ENSMUSP00000054856	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:qcr9_mouse(display_long)|uniprotkb:Uqcr10(gene name)|psi-mi:Uqcr10(display_short)|uniprotkb:Ubiquinol-cytochrome c reductase complex 7.2 kDa protein(gene name synonym)|uniprotkb:Cytochrome c1 non-heme 7 kDa protein(gene name synonym)|uniprotkb:Complex III subunit X(gene name synonym)|uniprotkb:Complex III subunit 9(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_932096.1|mint:Q8R1I1|reactome:R-MMU-611105|ensembl:ENSMUSG00000059534(gene)|ensembl:ENSMUST00000058407(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005750"(mitochondrial respiratory chain complex III)|go:"GO:0006122"(mitochondrial electron transport, ubiquinol to cytochrome c)|go:"GO:0009060"(aerobic respiration)|interpro:IPR008027(Ubiquinol-cytochrome C reductase, UQCRX/QCR9-like)|interpro:IPR036656	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:guk9NxhiqhaiTQvxTgWP+k+9NEY10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9R0P9	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-1635245|uniprotkb:Q9R122|ensembl:ENSMUSP00000031131	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:uchl1_mouse(display_long)|uniprotkb:Uchl1(gene name)|psi-mi:Uchl1(display_short)|uniprotkb:Ubiquitin thioesterase L1(gene name synonym)|uniprotkb:Neuron cytoplasmic protein 9.5(gene name synonym)|uniprotkb:PGP 9.5(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_035800.2|ensembl:ENSMUSG00000029223(gene)|ensembl:ENSMUST00000031131(transcript)|go:"GO:0002931"(response to ischemia)|go:"GO:0004197"(cysteine-type endopeptidase activity)|go:"GO:0004843"(thiol-dependent deubiquitinase)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0007409"(axonogenesis)|go:"GO:0007412"(axon target recognition)|go:"GO:0007628"(adult walking behavior)|go:"GO:0008242"(omega peptidase activity)|go:"GO:0008283"(cell population proliferation)|go:"GO:0016579"(protein deubiquitination)|go:"GO:0016874"(ligase activity)|go:"GO:0019896"(axonal transport of mitochondrion)|go:"GO:0030424"(axon)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0031694"(alpha-2A adrenergic receptor binding)|go:"GO:0035690"|go:"GO:0042755"(eating behavior)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043130"(ubiquitin binding)|go:"GO:0043209"(myelin sheath)|go:"GO:0043407"(negative regulation of MAP kinase activity)|go:"GO:0044306"(neuron projection terminus)|go:"GO:0050905"(neuromuscular process)|go:"GO:0055001"(muscle cell development)|go:"GO:1904115"(axon cytoplasm)|interpro:IPR001578(Peptidase C12, ubiquitin carboxyl-terminal hydrolase 1)|interpro:IPR030297|interpro:IPR036959|interpro:IPR038765|mint:Q9R0P9|reactome:R-MMU-5689603|go:"GO:0019233"(sensory perception of pain)	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:OwNZHaBcqFPb5UKgm9WtL6AXn/o10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P99024	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-350896|uniprotkb:P05218|uniprotkb:Q3TFB6|uniprotkb:Q3THH9|uniprotkb:Q3TIL1|uniprotkb:Q3UAV4|uniprotkb:Q3UF52|uniprotkb:Q8WUC1|uniprotkb:Q9CY33|uniprotkb:B1B178|ensembl:ENSMUSP00000001566	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:tbb5_mouse(display_long)|uniprotkb:Tubb5(gene name)|psi-mi:Tubb5(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_035785.1|interpro:IPR023123|interpro:IPR036525|interpro:IPR018316|interpro:IPR037103|mint:P99024|reactome:R-MMU-2565942|reactome:R-MMU-380259|reactome:R-MMU-380270|reactome:R-MMU-380284|reactome:R-MMU-380320|reactome:R-MMU-5620912|reactome:R-MMU-6798695|reactome:R-MMU-8854518|ensembl:ENSMUSG00000001525(gene)|ensembl:ENSMUST00000001566(transcript)|go:"GO:0000226"(microtubule cytoskeleton organization)|go:"GO:0000278"(mitotic cell cycle)|go:"GO:0005200"(structural constituent of cytoskeleton)|go:"GO:0005525"(GTP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005641"(nuclear envelope lumen)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005874"(microtubule)|go:"GO:0007017"(microtubule-based process)|go:"GO:0009987"(cellular process)|go:"GO:0019904"(protein domain specific binding)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0032794"(GTPase activating protein binding)|go:"GO:0032991"(protein-containing complex)|go:"GO:0036464"(cytoplasmic ribonucleoprotein granule)|go:"GO:0042288"(MHC class I protein binding)|go:"GO:0044297"(cell body)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045121"(membrane raft)|go:"GO:0045298"(tubulin complex)|go:"GO:0050807"(regulation of synapse organization)|go:"GO:0051225"(spindle assembly)|interpro:IPR000217(Tubulin)|interpro:IPR002453(Beta tubulin)|interpro:IPR003008(Tubulin/FtsZ, GTPase)|interpro:IPR008280(Tubulin/FtsZ, C-terminal)|interpro:IPR013838(Beta tubulin, autoregulation binding site)|interpro:IPR017975	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:B6pmb1dvCroZXGd/1Zazi+s72GA10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P68372	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-2553564|uniprotkb:P05217|uniprotkb:Q3UJ73|uniprotkb:Q3TKF0|uniprotkb:Q99JZ6|ensembl:ENSMUSP00000042342	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:tbb4b_mouse(display_long)|uniprotkb:Tubb4b(gene name)|psi-mi:Tubb4b(display_short)|uniprotkb:Tubb2c(gene name synonym)|uniprotkb:Tubulin beta-2C chain(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000036752(gene)|ensembl:ENSMUST00000043584(transcript)|go:"GO:0000226"(microtubule cytoskeleton organization)|go:"GO:0000278"(mitotic cell cycle)|go:"GO:0003725"(double-stranded RNA binding)|go:"GO:0005200"(structural constituent of cytoskeleton)|go:"GO:0005525"(GTP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005874"(microtubule)|go:"GO:0007017"(microtubule-based process)|go:"GO:0043209"(myelin sheath)|go:"GO:0045298"(tubulin complex)|interpro:IPR000217(Tubulin)|interpro:IPR002453(Beta tubulin)|interpro:IPR003008(Tubulin/FtsZ, GTPase)|interpro:IPR008280(Tubulin/FtsZ, C-terminal)|interpro:IPR013838(Beta tubulin, autoregulation binding site)|interpro:IPR017975|interpro:IPR018316|interpro:IPR023123|interpro:IPR036525|interpro:IPR037103|mint:P68372|reactome:R-MMU-190840|reactome:R-MMU-2132295|reactome:R-MMU-2467813|reactome:R-MMU-2500257|reactome:R-MMU-2565942|reactome:R-MMU-3371497|reactome:R-MMU-380259|reactome:R-MMU-380270|reactome:R-MMU-380284|reactome:R-MMU-380320|reactome:R-MMU-437239|reactome:R-MMU-5610787|reactome:R-MMU-5617833|reactome:R-MMU-5620912|reactome:R-MMU-5620924|reactome:R-MMU-5626467|reactome:R-MMU-5663220|reactome:R-MMU-6798695|reactome:R-MMU-6807878|reactome:R-MMU-6811434|reactome:R-MMU-6811436|reactome:R-MMU-68877|reactome:R-MMU-8852276|reactome:R-MMU-8854518|reactome:R-MMU-8955332|reactome:R-MMU-9646399|reactome:R-MMU-9648025|reactome:R-MMU-9668328|reactome:R-MMU-983189|refseq:NP_666228.1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:4LwMs7E7eILMjB9Z/1bstAp22CY10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9CWF2	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-2794256|uniprotkb:Q3TG26|ensembl:ENSMUSP00000075178	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:tbb2b_mouse(display_long)|uniprotkb:Tubb2b(gene name)|psi-mi:Tubb2b(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_076205.1|go:"GO:0050804"(modulation of chemical synaptic transmission)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:1902669"(positive regulation of axon guidance)|go:"GO:1990403"(embryonic brain development)|interpro:IPR000217(Tubulin)|interpro:IPR002453(Beta tubulin)|interpro:IPR003008(Tubulin/FtsZ, GTPase)|interpro:IPR008280(Tubulin/FtsZ, C-terminal)|interpro:IPR013838(Beta tubulin, autoregulation binding site)|interpro:IPR017975|interpro:IPR018316|interpro:IPR023123|interpro:IPR036525|interpro:IPR037103|mint:Q9CWF2|reactome:R-MMU-190840|reactome:R-MMU-2132295|reactome:R-MMU-2467813|reactome:R-MMU-2500257|reactome:R-MMU-3371497|reactome:R-MMU-380320|reactome:R-MMU-437239|reactome:R-MMU-5610787|reactome:R-MMU-5617833|reactome:R-MMU-5620924|reactome:R-MMU-5626467|reactome:R-MMU-5663220|reactome:R-MMU-6807878|reactome:R-MMU-6811434|reactome:R-MMU-6811436|reactome:R-MMU-9646399|reactome:R-MMU-9648025|reactome:R-MMU-9668328|reactome:R-MMU-983189|reactome:R-MMU-68877|reactome:R-MMU-8852276|reactome:R-MMU-8955332|ensembl:ENSMUSG00000045136(gene)|ensembl:ENSMUST00000075774(transcript)|go:"GO:0000226"(microtubule cytoskeleton organization)|go:"GO:0000278"(mitotic cell cycle)|go:"GO:0001764"(neuron migration)|go:"GO:0005200"(structural constituent of cytoskeleton)|go:"GO:0005525"(GTP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005874"(microtubule)|go:"GO:0007017"(microtubule-based process)|go:"GO:0015630"(microtubule cytoskeleton)|go:"GO:0046982"(protein heterodimerization activity)	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:sluQMryNuOvp6xlV558+agiRwvE10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q7TMM9	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-2255578|ensembl:ENSMUSP00000060246	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:tbb2a_mouse(display_long)|uniprotkb:Tubb2(gene name synonym)|uniprotkb:Tubb2a(gene name)|psi-mi:Tubb2a(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_033476.1|ensembl:ENSMUSG00000058672(gene)|ensembl:ENSMUST00000056427(transcript)|go:"GO:0000226"(microtubule cytoskeleton organization)|go:"GO:0000278"(mitotic cell cycle)|go:"GO:0001764"(neuron migration)|go:"GO:0005200"(structural constituent of cytoskeleton)|go:"GO:0005525"(GTP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005874"(microtubule)|interpro:IPR000217(Tubulin)|interpro:IPR002453(Beta tubulin)|interpro:IPR003008(Tubulin/FtsZ, GTPase)|interpro:IPR008280(Tubulin/FtsZ, C-terminal)|interpro:IPR013838(Beta tubulin, autoregulation binding site)|interpro:IPR017975|interpro:IPR018316|interpro:IPR023123|interpro:IPR036525|interpro:IPR037103|mint:Q7TMM9|reactome:R-MMU-190840|reactome:R-MMU-2132295|reactome:R-MMU-2467813|reactome:R-MMU-2500257|reactome:R-MMU-3371497|reactome:R-MMU-380320|reactome:R-MMU-437239|reactome:R-MMU-5610787|reactome:R-MMU-5617833|reactome:R-MMU-5620924|reactome:R-MMU-5626467|reactome:R-MMU-5663220|reactome:R-MMU-6807878|reactome:R-MMU-6811434|reactome:R-MMU-6811436|reactome:R-MMU-68877|reactome:R-MMU-8852276|reactome:R-MMU-8955332|reactome:R-MMU-9646399|reactome:R-MMU-9648025|reactome:R-MMU-9668328|reactome:R-MMU-983189	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:XN9uTGU0KxQjMod0AGnM+XJoHyk10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q3UX10	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-6910149|ensembl:ENSMUSP00000021639|uniprotkb:B9EJS3	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:tbal3_mouse(display_long)|uniprotkb:Tubal3(gene name)|psi-mi:Tubal3(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000021216(gene)|ensembl:ENSMUST00000021639(transcript)|go:"GO:0000226"(microtubule cytoskeleton organization)|go:"GO:0000278"(mitotic cell cycle)|go:"GO:0005200"(structural constituent of cytoskeleton)|go:"GO:0005525"(GTP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005874"(microtubule)|interpro:IPR000217(Tubulin)|interpro:IPR002452(Alpha tubulin)|interpro:IPR003008(Tubulin/FtsZ, GTPase)|interpro:IPR008280(Tubulin/FtsZ, C-terminal)|interpro:IPR017975|interpro:IPR018316|interpro:IPR023123|interpro:IPR036525|interpro:IPR037103|mint:Q3UX10|reactome:R-MMU-190840|reactome:R-MMU-2132295|reactome:R-MMU-2467813|reactome:R-MMU-2500257|reactome:R-MMU-3371497|reactome:R-MMU-380320|reactome:R-MMU-437239|reactome:R-MMU-5617833|reactome:R-MMU-5626467|reactome:R-MMU-5663220|reactome:R-MMU-6807878|reactome:R-MMU-6811434|reactome:R-MMU-6811436|reactome:R-MMU-68877|reactome:R-MMU-8852276|reactome:R-MMU-8955332|reactome:R-MMU-9646399|reactome:R-MMU-9648025|reactome:R-MMU-9668328|reactome:R-MMU-983189|refseq:NP_001029051.2	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:jbpZr3Dct0cMp2lQFmaEBQO81zI10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9QZ06	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-74272|uniprotkb:Q543N1|uniprotkb:Q9D5P5|ensembl:ENSMUSP00000001950	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:tolip_mouse(display_long)|uniprotkb:Tollip(gene name)|psi-mi:Tollip(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_076253.1|ensembl:ENSMUSG00000025139(gene)|ensembl:ENSMUST00000001950(transcript)|go:"GO:0005150"(interleukin-1, type I receptor binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0006914"(autophagy)|go:"GO:0006954"(inflammatory response)|go:"GO:0007165"(signal transduction)|go:"GO:0016310"(phosphorylation)|go:"GO:0016604"(nuclear body)|go:"GO:0019900"(kinase binding)|go:"GO:0030855"(epithelial cell differentiation)|go:"GO:0031624"(ubiquitin conjugating enzyme binding)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0032183"(SUMO binding)|go:"GO:0032991"(protein-containing complex)|go:"GO:0033235"(positive regulation of protein sumoylation)|go:"GO:0035325"(Toll-like receptor binding)|go:"GO:0036010"(protein localization to endosome)|go:"GO:0043130"(ubiquitin binding)|go:"GO:0045087"(innate immune response)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0070498"(interleukin-1-mediated signaling pathway)|interpro:IPR000008(C2 calcium-dependent membrane targeting)|interpro:IPR003892(Ubiquitin system component Cue)|interpro:IPR009060(UBA-like)|interpro:IPR035892|interpro:IPR037301|interpro:IPR041799|mint:Q9QZ06|reactome:R-MMU-6798695|reactome:R-MMU-9020702	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:l31YAZvuxJMK48wHqwvgPu8lVUs10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q8BYI9	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-774167|uniprotkb:A2RT70|uniprotkb:O88717|ensembl:ENSMUSP00000107298|ensembl:ENSMUSP00000141553	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:tenr_mouse(display_long)|uniprotkb:Restrictin(gene name synonym)|uniprotkb:Janusin(gene name synonym)|uniprotkb:Neural recognition molecule J1-160/180(gene name synonym)|uniprotkb:Tnr(gene name)|psi-mi:Tnr(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_071707.2|refseq:XP_006496805.1|refseq:XP_006496806.1|refseq:XP_006496807.1|ensembl:ENSMUSG00000015829(gene)|ensembl:ENSMUST00000111669(transcript)|ensembl:ENSMUST00000192069(transcript)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005178"(integrin binding)|go:"GO:0005615"(extracellular space)|go:"GO:0007155"(cell adhesion)|go:"GO:0007158"(neuron cell-cell adhesion)|go:"GO:0007162"(negative regulation of cell adhesion)|go:"GO:0008306"(associative learning)|go:"GO:0009986"(cell surface)|go:"GO:0010977"(negative regulation of neuron projection development)|go:"GO:0022029"(telencephalon cell migration)|go:"GO:0022408"(negative regulation of cell-cell adhesion)|go:"GO:0030198"(extracellular matrix organization)|go:"GO:0030517"(negative regulation of axon extension)|go:"GO:0031012"(extracellular matrix)|go:"GO:0035641"(locomotory exploration behavior)|go:"GO:0045121"(membrane raft)|go:"GO:0046625"(sphingolipid binding)|go:"GO:0048692"(negative regulation of axon extension involved in regeneration)|go:"GO:0050804"(modulation of chemical synaptic transmission)|go:"GO:0050805"(negative regulation of synaptic transmission)|go:"GO:0050808"(synapse organization)|go:"GO:0050885"(neuromuscular process controlling balance)|go:"GO:0051968"(positive regulation of synaptic transmission, glutamatergic)|go:"GO:0051971"(positive regulation of transmission of nerve impulse)|go:"GO:0060291"(long-term synaptic potentiation)|go:"GO:0062023"(collagen-containing extracellular matrix)|go:"GO:0072534"(perineuronal net)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098966"(perisynaptic extracellular matrix)|go:"GO:0098978"(glutamatergic synapse)|interpro:IPR000742(EGF-like, type 3)|interpro:IPR002181(Fibrinogen, alpha/beta/gamma chain, C-terminal globular)|interpro:IPR003961(Fibronectin, type III)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR014716(Fibrinogen, alpha/beta/gamma chain, C-terminal globular, subdomain 1)|interpro:IPR033079|interpro:IPR036056|interpro:IPR036116|interpro:IPR041161|mint:Q8BYI9|reactome:R-MMU-3000178	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:K7s7yPGS4RvIe+thCkAZIFGjNRA10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:F6SEU4	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-5797569|ensembl:ENSMUSP00000141686	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:sygp1_mouse(display_long)|uniprotkb:Syngap1(gene name)|psi-mi:Syngap1(display_short)|uniprotkb:Neuronal RasGAP(gene name synonym)|uniprotkb:Synaptic Ras GTPase-activating protein 1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001268420.1|ensembl:ENSMUSG00000067629(gene)|ensembl:ENSMUST00000194598(transcript)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0007265"(Ras protein signal transduction)|go:"GO:0007389"(pattern specification process)|go:"GO:0008542"(visual learning)|go:"GO:0014069"(postsynaptic density)|go:"GO:0016020"(membrane)|go:"GO:0016358"(dendrite development)|go:"GO:0017124"(SH3 domain binding)|go:"GO:0019901"(protein kinase binding)|go:"GO:0043087"(regulation of GTPase activity)|go:"GO:0043113"(receptor clustering)|go:"GO:0043198"(dendritic shaft)|go:"GO:0043408"(regulation of MAPK cascade)|go:"GO:0043524"(negative regulation of neuron apoptotic process)|go:"GO:0045202"(synapse)|go:"GO:0046580"(negative regulation of Ras protein signal transduction)|go:"GO:0048167"(regulation of synaptic plasticity)|go:"GO:0048169"(regulation of long-term neuronal synaptic plasticity)|go:"GO:0050771"(negative regulation of axonogenesis)|go:"GO:0050803"(regulation of synapse structure or activity)|go:"GO:0098880"(maintenance of postsynaptic specialization structure)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|interpro:IPR000008(C2 calcium-dependent membrane targeting)|interpro:IPR001849(Pleckstrin-like)|interpro:IPR001936(Ras GTPase-activating protein)|interpro:IPR008936(Rho GTPase activation protein)|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR021887|interpro:IPR023152|interpro:IPR035892|interpro:IPR037779|interpro:IPR039360|mint:F6SEU4|rcsb pdb:5JXC|reactome:R-MMU-5658442	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:X1QOpwn0EU5av7G5/QRYmQopTdM10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q7TMK9	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-2735641|uniprotkb:O88991|uniprotkb:Q2YDV8|uniprotkb:Q68ED6|uniprotkb:Q80YV6|uniprotkb:Q8BGP1|uniprotkb:Q8C5K6|uniprotkb:Q8CGC2|uniprotkb:Q91ZR0|uniprotkb:Q99KU9|uniprotkb:Q9QYF4|ensembl:ENSMUSP00000133649	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:hnrpq_mouse(display_long)|uniprotkb:Syncrip(gene name)|psi-mi:Syncrip(display_short)|uniprotkb:Hnrpq(gene name synonym)|uniprotkb:Nsap1(gene name synonym)|uniprotkb:Nsap1l(gene name synonym)|uniprotkb:Synaptotagmin-binding, cytoplasmic RNA-interacting protein(gene name synonym)|uniprotkb:Glycine- and tyrosine-rich RNA-binding protein(gene name synonym)|uniprotkb:NS1-associated protein 1(gene name synonym)|uniprotkb:pp68(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001271257.1|refseq:NP_062640.2|refseq:NP_062770.1|refseq:NP_001298042.1|ensembl:ENSMUSG00000032423(gene)|ensembl:ENSMUST00000173801(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003729"(mRNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0006397"(mRNA processing)|go:"GO:0008143"("poly(A) binding")|go:"GO:0008380"(RNA splicing)|go:"GO:0017148"(negative regulation of translation)|go:"GO:0048027"(mRNA 5'-UTR binding)|go:"GO:0070934"(CRD-mediated mRNA stabilization)|go:"GO:0070937"(CRD-mediated mRNA stability complex)|go:"GO:0071013"(catalytic step 2 spliceosome)|go:"GO:0071204"(histone pre-mRNA 3'end processing complex)|go:"GO:0071346"(cellular response to interferon-gamma)|go:"GO:0097452"(GAIT complex)|go:"GO:1990904"(ribonucleoprotein complex)|interpro:IPR000504(RNA recognition motif, RNP-1)|interpro:IPR006535(HnRNP R and Q splicing factor)|interpro:IPR012677(Nucleotide-binding, alpha-beta plait)|interpro:IPR034544|interpro:IPR034548|interpro:IPR035979|interpro:IPR041337|mint:Q7TMK9	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:Ljuz8koebOL7L9jFBaevQYHinzw10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9WUD1	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-773027|uniprotkb:Q9DCJ0|ensembl:ENSMUSP00000040431	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:chip_mouse(display_long)|uniprotkb:Carboxy terminus of Hsp70-interacting protein(gene name synonym)|uniprotkb:Stub1(gene name)|psi-mi:Stub1(display_short)|uniprotkb:Chip(gene name synonym)|uniprotkb:RING-type E3 ubiquitin transferase CHIP(gene name synonym)|uniprotkb:STIP1 homology and U box-containing protein 1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_062693.1|dip:DIP-29751N|ensembl:ENSMUSG00000039615(gene)|ensembl:ENSMUST00000044911(transcript)|go:"GO:0000151"(ubiquitin ligase complex)|go:"GO:0000209"(protein polyubiquitination)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0002931"(response to ischemia)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005829"(cytosol)|go:"GO:0006281"(DNA repair)|go:"GO:0006457"(protein folding)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0006515"(protein quality control for misfolded or incompletely synthesized proteins)|go:"GO:0016567"(protein ubiquitination)|go:"GO:0019899"(enzyme binding)|go:"GO:0019900"(kinase binding)|go:"GO:0030018"(Z disc)|go:"GO:0030433"(ubiquitin-dependent ERAD pathway)|go:"GO:0030512"(negative regulation of transforming growth factor beta receptor signaling pathway)|go:"GO:0030544"(Hsp70 protein binding)|go:"GO:0030579"(ubiquitin-dependent SMAD protein catabolic process)|go:"GO:0030674"(protein-macromolecule adaptor activity)|go:"GO:0030911"(TPR domain binding)|go:"GO:0030968"(endoplasmic reticulum unfolded protein response)|go:"GO:0031072"(heat shock protein binding)|go:"GO:0031371"(ubiquitin conjugating enzyme complex)|go:"GO:0031398"(positive regulation of protein ubiquitination)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0031647"(regulation of protein stability)|go:"GO:0031943"(regulation of glucocorticoid metabolic process)|go:"GO:0032091"(negative regulation of protein binding)|go:"GO:0032436"(positive regulation of proteasomal ubiquitin-dependent protein catabolic process)|go:"GO:0034450"(ubiquitin-ubiquitin ligase activity)|go:"GO:0034605"(cellular response to heat)|go:"GO:0042405"(nuclear inclusion body)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0043161"(proteasome-mediated ubiquitin-dependent protein catabolic process)|go:"GO:0045862"(positive regulation of proteolysis)|go:"GO:0046332"(SMAD binding)|go:"GO:0051087"(chaperone binding)|go:"GO:0051443"(positive regulation of ubiquitin-protein transferase activity)|go:"GO:0051604"(protein maturation)|go:"GO:0051787"(misfolded protein binding)|go:"GO:0051865"(protein autoubiquitination)|go:"GO:0051879"(Hsp90 protein binding)|go:"GO:0061630"(ubiquitin protein ligase activity)|go:"GO:0061684"(chaperone-mediated autophagy)|go:"GO:0070534"(protein K63-linked ubiquitination)|go:"GO:0071218"(cellular response to misfolded protein)|go:"GO:0071456"(cellular response to hypoxia)|go:"GO:0090035"(positive regulation of chaperone-mediated protein complex assembly)|go:"GO:0101031"(chaperone complex)|interpro:IPR003613(U box)|interpro:IPR011990(Tetratricopeptide-like helical)|interpro:IPR013083(Zinc finger, RING/FYVE/PHD-type)|interpro:IPR019734|interpro:IPR041312|mint:Q9WUD1|rcsb pdb:2C2L|rcsb pdb:2C2V|rcsb pdb:3Q47|rcsb pdb:3Q49|rcsb pdb:3Q4A|reactome:R-MMU-2173788|reactome:R-MMU-5675482|reactome:R-MMU-8863795|reactome:R-MMU-8939902|reactome:R-MMU-8948751|reactome:R-MMU-983168	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:C5WR6X2IRpYeSijSpib3cquUaBQ10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:O55106	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-2479958|uniprotkb:B2RWV9|uniprotkb:Q5BKR9|ensembl:ENSMUSP00000120830	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:strn_mouse(display_long)|uniprotkb:Strn(gene name)|psi-mi:Strn(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000024077(gene)|ensembl:ENSMUST00000145910(transcript)|go:"GO:0005516"(calmodulin binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005923"(bicellular tight junction)|go:"GO:0007626"(locomotory behavior)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0016020"(membrane)|go:"GO:0016055"(Wnt signaling pathway)|go:"GO:0016358"(dendrite development)|go:"GO:0019904"(protein domain specific binding)|go:"GO:0030331"(estrogen receptor binding)|go:"GO:0030425"(dendrite)|go:"GO:0032991"(protein-containing complex)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043197"(dendritic spine)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0051721"(protein phosphatase 2A binding)|go:"GO:0070016"(armadillo repeat domain binding)|go:"GO:0090443"(FAR/SIN/STRIPAK complex)|interpro:IPR001680(WD40 repeat)|interpro:IPR013258(Striatin, N-terminal)|go:"GO:0014069"(postsynaptic density)|interpro:IPR019775|interpro:IPR020472|interpro:IPR036322|mint:O55106|reactome:R-MMU-1257604|reactome:R-MMU-6811558|reactome:R-MMU-9009391|interpro:IPR015943(WD40/YVTN repeat-like)|refseq:NP_035630.2	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:tUJDyyIWZKuEBcyJKUIbk2dR4JE10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q91Z67	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-16730393|ensembl:ENSMUSP00000095195|uniprotkb:B2RY13|uniprotkb:Q3V1V8|uniprotkb:Q61054	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:srgp2_mouse(display_long)|uniprotkb:Formin-binding protein 2(gene name synonym)|uniprotkb:Formin-binding protein 27(gene name synonym)|uniprotkb:Srgap2(gene name)|psi-mi:Srgap2(display_short)|uniprotkb:Fbp27(gene name synonym)|uniprotkb:Fnbp2(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000026425(gene)|ensembl:ENSMUST00000097588(transcript)|go:"GO:0003363"(lamellipodium assembly involved in ameboidal cell migration)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0007165"(signal transduction)|go:"GO:0014069"(postsynaptic density)|go:"GO:0021816"(extension of a leading process involved in cell motility in cerebral cortex radial glia guided migration)|go:"GO:0030027"(lamellipodium)|go:"GO:0030336"(negative regulation of cell migration)|go:"GO:0031267"(small GTPase binding)|go:"GO:0034446"(substrate adhesion-dependent cell spreading)|go:"GO:0042802"(identical protein binding)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0043547"(positive regulation of GTPase activity)|go:"GO:0044327"(dendritic spine head)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0045335"(phagocytic vesicle)|go:"GO:0046847"(filopodium assembly)|go:"GO:0048812"(neuron projection morphogenesis)|go:"GO:0051014"(actin filament severing)|go:"GO:0060996"(dendritic spine development)|go:"GO:2001223"(negative regulation of neuron migration)|interpro:IPR000198(RhoGAP)|interpro:IPR001060(Cdc15/Fes/CIP4)|interpro:IPR001452(Src homology-3)|interpro:IPR008936(Rho GTPase activation protein)|interpro:IPR027267|interpro:IPR030252|interpro:IPR031160|interpro:IPR035648|interpro:IPR036028|mint:Q91Z67|reactome:R-MMU-5663220|reactome:R-MMU-9013148|reactome:R-MMU-9013149|reactome:R-MMU-9013406|reactome:R-MMU-9013420|reactome:R-MMU-9013423|reactome:R-MMU-9035034|refseq:NP_001074480.2|refseq:XP_006529197.1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:MLCAa8wEQvKNPatehgSzcizs2YI10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9QWI6	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-775592|uniprotkb:O70298	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:srcn1_mouse(display_long)|uniprotkb:p140Cap(gene name synonym)|uniprotkb:SNAP-25-interacting protein(gene name synonym)|uniprotkb:Srcin1(gene name)|psi-mi:Srcin1(display_short)|uniprotkb:Kiaa1684(gene name synonym)|uniprotkb:P140(gene name synonym)|uniprotkb:p130Cas-associated protein(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_061361.2|interpro:IPR022782|interpro:IPR026727|mint:Q9QWI6|go:"GO:0005737"(cytoplasm)|go:"GO:0006887"(exocytosis)|go:"GO:0007162"(negative regulation of cell adhesion)|go:"GO:0014069"(postsynaptic density)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019901"(protein kinase binding)|go:"GO:0019904"(protein domain specific binding)|go:"GO:0030027"(lamellipodium)|go:"GO:0030175"(filopodium)|go:"GO:0030234"(enzyme regulator activity)|go:"GO:0030334"(regulation of cell migration)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0034446"(substrate adhesion-dependent cell spreading)|go:"GO:0043005"(neuron projection)|go:"GO:0043025"(neuronal cell body)|go:"GO:0045202"(synapse)|go:"GO:0050709"(negative regulation of protein secretion)|go:"GO:0061001"(regulation of dendritic spine morphogenesis)|go:"GO:0061098"(positive regulation of protein tyrosine kinase activity)|go:"GO:0061099"(negative regulation of protein tyrosine kinase activity)|go:"GO:0098793"(presynapse)	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:M3+KJqcvaW0sMG63nFO9CXyjp9Q10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q3UHD6	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-6910393|ensembl:ENSMUSP00000102904|uniprotkb:Q7TQL6|uniprotkb:Q80TZ1|uniprotkb:Q9CYB5	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:snx27_mouse(display_long)|uniprotkb:Snx27(gene name)|psi-mi:Snx27(display_short)|uniprotkb:Kiaa0488(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	go:"GO:0006886"(intracellular protein transport)|go:"GO:0007165"(signal transduction)|go:"GO:0008333"(endosome to lysosome transport)|go:"GO:0014069"(postsynaptic density)|go:"GO:0016197"(endosomal transport)|go:"GO:0030904"(retromer complex)|ensembl:ENSMUSG00000028136(gene)|ensembl:ENSMUST00000107283(transcript)|go:"GO:0001772"(immunological synapse)|go:"GO:0031901"(early endosome membrane)|go:"GO:0032266"(phosphatidylinositol-3-phosphate binding)|go:"GO:0032456"(endocytic recycling)|go:"GO:0035091"(phosphatidylinositol binding)|go:"GO:0005654"(nucleoplasm)|go:"GO:0035255"(ionotropic glutamate receptor binding)|go:"GO:0061951"(establishment of protein localization to plasma membrane)|go:"GO:0071203"(WASH complex)|go:"GO:0005768"(endosome)|go:"GO:0099638"(endosome to plasma membrane protein transport)|go:"GO:1903609"(negative regulation of inward rectifier potassium channel activity)|go:"GO:1904719"(positive regulation of AMPA glutamate receptor clustering)|interpro:IPR000159(Ras-association)|interpro:IPR001478(PDZ/DHR/GLGF)|go:"GO:0005769"(early endosome)|interpro:IPR001683(Phox-like)|interpro:IPR028667|interpro:IPR029071|go:"GO:0005829"(cytosol)|interpro:IPR036034|go:"GO:0005886"(plasma membrane)|interpro:IPR036871|interpro:IPR037827|interpro:IPR037831|interpro:IPR037833|interpro:IPR037835|mint:Q3UHD6|refseq:NP_001075953.1|refseq:NP_083997.1|dip:DIP-60337N	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:+pFe87lSKQPJuMDWsiMU4iXhGOw10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q91VE0	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-26596020|ensembl:ENSMUSP00000078971|uniprotkb:O88562|uniprotkb:Q3TD48	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:s27a4_mouse(display_long)|uniprotkb:Arachidonate--CoA ligase(gene name synonym)|uniprotkb:Long-chain-fatty-acid--CoA ligase(gene name synonym)|uniprotkb:Solute carrier family 27 member 4(gene name synonym)|uniprotkb:Very long-chain acyl-CoA synthetase 4(gene name synonym)|uniprotkb:Slc27a4(gene name)|psi-mi:Slc27a4(display_short)|uniprotkb:Acsvl4(gene name synonym)|uniprotkb:Fatp4(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000059316(gene)|ensembl:ENSMUST00000080065(transcript)|go:"GO:0000038"(very long-chain fatty acid metabolic process)|go:"GO:0000166"(nucleotide binding)|go:"GO:0001579"(medium-chain fatty acid transport)|go:"GO:0001676"(long-chain fatty acid metabolic process)|go:"GO:0004467"(long-chain fatty acid-CoA ligase activity)|go:"GO:0005324"(long-chain fatty acid transporter activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005902"(microvillus)|go:"GO:0006631"(fatty acid metabolic process)|go:"GO:0007584"(response to nutrient)|go:"GO:0015908"(fatty acid transport)|go:"GO:0015909"(long-chain fatty acid transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031526"(brush border membrane)|go:"GO:0031957"(very long-chain fatty acid-CoA ligase activity)|go:"GO:0042760"(very long-chain fatty acid catabolic process)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043588"(skin development)|go:"GO:0044381"(glucose import in response to insulin stimulus)|go:"GO:0044539"(long-chain fatty acid import into cell)|go:"GO:0046627"(negative regulation of insulin receptor signaling pathway)|go:"GO:0047676"(arachidonate-CoA ligase activity)|go:"GO:0062003"(negative regulation of all-trans-retinyl-ester hydrolase, 11-cis retinol forming activity)|go:"GO:0090433"(palmitoyl-CoA ligase activity)|go:"GO:0090434"(oleoyl-CoA ligase activity)|go:"GO:0090630"(activation of GTPase activity)|go:"GO:1901480"(oleate transmembrane transporter activity)|go:"GO:1990379"(lipid transport across blood-brain barrier)|interpro:IPR000873(AMP-dependent synthetase and ligase)|interpro:IPR020845|interpro:IPR022272|interpro:IPR025110|interpro:IPR030304|interpro:IPR042099|mint:Q91VE0|reactome:R-MMU-804914|refseq:NP_036119.1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:K6elkQxCl5bz5X01ErBVDSK9L9A10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q62420	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-77971|ensembl:ENSMUSP00000030212|uniprotkb:Q3UTL7|uniprotkb:Q3UY49|uniprotkb:Q8BXS6|uniprotkb:Q8VBV1	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:sh3g2_mouse(display_long)|uniprotkb:Sh3gl2(gene name)|psi-mi:Sh3gl2(display_short)|uniprotkb:Sh3d2a(gene name synonym)|uniprotkb:Endophilin-1(gene name synonym)|uniprotkb:SH3 domain-containing GRB2-like protein 2(gene name synonym)|uniprotkb:SH3 domain protein 2A(gene name synonym)|uniprotkb:Een1(gene name synonym)|uniprotkb:SH3p4(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	interpro:IPR035824|interpro:IPR036028|mint:Q62420|rcsb pdb:1ZWW|reactome:R-MMU-177504|reactome:R-MMU-182971|reactome:R-MMU-2132295|reactome:R-MMU-432720|reactome:R-MMU-432722|reactome:R-MMU-437239|reactome:R-MMU-6807004|reactome:R-MMU-8856825|reactome:R-MMU-8856828|go:"GO:0008022"(protein C-terminus binding)|ensembl:ENSMUSG00000028488(gene)|ensembl:ENSMUST00000030212(transcript)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0002090"(regulation of receptor internalization)|go:"GO:0005769"(early endosome)|go:"GO:0005829"(cytosol)|go:"GO:0008289"(lipid binding)|go:"GO:0010629"(negative regulation of gene expression)|go:"GO:0016191"(synaptic vesicle uncoating)|go:"GO:0019901"(protein kinase binding)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0031175"(neuron projection development)|go:"GO:0042802"(identical protein binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0045202"(synapse)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0048488"(synaptic vesicle endocytosis)|go:"GO:0060988"(lipid tube assembly)|go:"GO:0097441"(basal dendrite)|go:"GO:0097484"(dendrite extension)|go:"GO:0097749"(membrane tubulation)|go:"GO:0097753"(membrane bending)|go:"GO:0098684"(photoreceptor ribbon synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098793"(presynapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099050"(vesicle scission)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:1903527"(positive regulation of membrane tubulation)|go:"GO:1905604"(negative regulation of blood-brain barrier permeability)|go:"GO:1990416"(cellular response to brain-derived neurotrophic factor stimulus)|go:"GO:2000369"(regulation of clathrin-dependent endocytosis)|interpro:IPR001452(Src homology-3)|interpro:IPR004148(BAR)|interpro:IPR027267|refseq:NP_062408.2|dip:DIP-30992N	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:hAkiLVjGXejkjd5pRcuq9wVXrfk10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:E9Q401	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-643628|ensembl:ENSMUSP00000021750|uniprotkb:O70181|uniprotkb:Q62174|uniprotkb:Q62197|uniprotkb:Q9ERN6	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:ryr2_mouse(display_long)|uniprotkb:Ryr2(gene name)|psi-mi:Ryr2(display_short)|uniprotkb:Cardiac muscle ryanodine receptor(gene name synonym)|uniprotkb:Cardiac muscle ryanodine receptor-calcium release channel(gene name synonym)|uniprotkb:Type 2 ryanodine receptor(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_076357.2|go:"GO:0071872"(cellular response to epinephrine stimulus)|go:"GO:0072599"(establishment of protein localization to endoplasmic reticulum)|go:"GO:0086005"(ventricular cardiac muscle cell action potential)|go:"GO:0086029"(Purkinje myocyte to ventricular cardiac muscle cell signaling)|go:"GO:0097050"(type B pancreatic cell apoptotic process)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0071421"(manganese ion transmembrane transport)|go:"GO:0097159"(organic cyclic compound binding)|go:"GO:0098735"(positive regulation of the force of heart contraction)|go:"GO:0098904"(regulation of AV node cell action potential)|go:"GO:0098907"(regulation of SA node cell action potential)|go:"GO:0098910"(regulation of atrial cardiac muscle cell action potential)|go:"GO:0098911"(regulation of ventricular cardiac muscle cell action potential)|go:"GO:1901896"(positive regulation of ATPase-coupled calcium transmembrane transporter activity)|interpro:IPR000699(Intracellular calcium-release channel)|interpro:IPR001870("B302 (SPRY)-like")|interpro:IPR002048(Calcium-binding EF-hand)|interpro:IPR003032(Ryanodine receptor Ryr)|interpro:IPR003877(SPla/RYanodine receptor SPRY)|interpro:IPR005821(Ion transport)|interpro:IPR009460(Ryanodine Receptor TM 4-6)|interpro:IPR011992(EF-Hand type)|go:"GO:0005886"(plasma membrane)|go:"GO:0006816"(calcium ion transport)|go:"GO:0006874"(cellular calcium ion homeostasis)|go:"GO:0010460"(positive regulation of heart rate)|go:"GO:0010881"(regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion)|go:"GO:0010882"(regulation of cardiac muscle contraction by calcium ion signaling)|go:"GO:0014808"(release of sequestered calcium ion into cytosol by sarcoplasmic reticulum)|go:"GO:0014850"(response to muscle activity)|go:"GO:0015278"(calcium-release channel activity)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016529"(sarcoplasmic reticulum)|go:"GO:0019722"(calcium-mediated signaling)|go:"GO:0019899"(enzyme binding)|go:"GO:0019901"(protein kinase binding)|go:"GO:0030017"(sarcomere)|go:"GO:0030018"(Z disc)|go:"GO:0030659"(cytoplasmic vesicle membrane)|go:"GO:0031000"(response to caffeine)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0031672"(A band)|go:"GO:0032026"(response to magnesium ion)|go:"GO:0032991"(protein-containing complex)|go:"GO:0033017"(sarcoplasmic reticulum membrane)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0034236"(protein kinase A catalytic subunit binding)|go:"GO:0034237"(protein kinase A regulatory subunit binding)|go:"GO:0034704"(calcium channel complex)|go:"GO:0035584"(calcium-mediated signaling using intracellular calcium source)|go:"GO:0035994"(response to muscle stretch)|go:"GO:0042383"(sarcolemma)|go:"GO:0042802"(identical protein binding)|go:"GO:0043005"(neuron projection)|go:"GO:0043621"(protein self-association)|go:"GO:0043924"(suramin binding)|go:"GO:0048763"(calcium-induced calcium release activity)|go:"GO:0051209"(release of sequestered calcium ion into cytosol)|go:"GO:0051284"(positive regulation of sequestering of calcium ion)|go:"GO:0051480"(regulation of cytosolic calcium ion concentration)|go:"GO:0051481"(negative regulation of cytosolic calcium ion concentration)|go:"GO:0051592"(response to calcium ion)|go:"GO:0051775"(response to redox state)|go:"GO:0055117"(regulation of cardiac muscle contraction)|go:"GO:0060048"(cardiac muscle contraction)|go:"GO:0060401"(cytosolic calcium ion transport)|go:"GO:0060402"(calcium ion transport into cytosol)|go:"GO:0070296"(sarcoplasmic reticulum calcium ion transport)|go:"GO:0070588"(calcium ion transmembrane transport)|go:"GO:0071313"(cellular response to caffeine)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|rcsb pdb:4ETV|rcsb pdb:4KEI|rcsb pdb:4KEJ|rcsb pdb:4KEK|rcsb pdb:4L4H|interpro:IPR013333(Ryanodine receptor, N-terminal)|rcsb pdb:4L4I|rcsb pdb:4P9I|interpro:IPR013662(RyR and IP3R Homology associated)|rcsb pdb:4P9L|interpro:IPR014821(Inositol 1,4,5-trisphosphate/ryanodine receptor)|rcsb pdb:5C33|rcsb pdb:5VSN|rcsb pdb:6J6L|rcsb pdb:6MM5|rcsb pdb:6MM6|rcsb pdb:6MM7|interpro:IPR016093(MIR motif)|rcsb pdb:6MM8|interpro:IPR035761|rcsb pdb:6WOU|rcsb pdb:6WOV|reactome:R-MMU-2672351|interpro:IPR035762|reactome:R-MMU-5578775|interpro:IPR035764|interpro:IPR035910|interpro:IPR036300|interpro:IPR043136|mint:E9Q401|rcsb pdb:2MC2|rcsb pdb:3IM5|rcsb pdb:3IM6|rcsb pdb:3IM7|rcsb pdb:3QR5|ensembl:ENSMUSG00000021313(gene)|ensembl:ENSMUST00000021750(transcript)|go:"GO:0001666"(response to hypoxia)|go:"GO:0002027"(regulation of heart rate)|go:"GO:0003143"(embryonic heart tube morphogenesis)|go:"GO:0003220"(left ventricular cardiac muscle tissue morphogenesis)|go:"GO:0003300"(cardiac muscle hypertrophy)|go:"GO:0005219"(ryanodine-sensitive calcium-release channel activity)|go:"GO:0005262"(calcium channel activity)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005513"(detection of calcium ion)|go:"GO:0005516"(calmodulin binding)|go:"GO:0005635"(nuclear envelope)|go:"GO:0005737"(cytoplasm)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005790"(smooth endoplasmic reticulum)	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:HHxj8k5HJe0bbt0o041H0inQo0w10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q99LF4	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-6261036|ensembl:ENSMUSP00000001834|uniprotkb:Q3UDW6|uniprotkb:Q3TI25|uniprotkb:Q3TA04	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:rtcb_mouse(display_long)|uniprotkb:Rtcb(gene name)|psi-mi:Rtcb(display_short)|uniprotkb:D10Wsu52e(gene name synonym)|uniprotkb:Focal adhesion-associated protein(gene name synonym)|uniprotkb:3'-phosphate/5'-hydroxy nucleic acid ligase(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000001783(gene)|go:"GO:0000971"(tRNA exon ligation utilizing 2',3' cyclic phosphate of 5'-exon as source of linkage phosphate)|go:"GO:0001701"(in utero embryonic development)|go:"GO:0001890"(placenta development)|go:"GO:0003972"("RNA ligase (ATP) activity")|go:"GO:0005525"(GTP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005635"(nuclear envelope)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005829"(cytosol)|go:"GO:0006388"(tRNA splicing, via endonucleolytic cleavage and ligation)|go:"GO:0017166"(vinculin binding)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046872"(metal ion binding)|go:"GO:0072669"(tRNA-splicing ligase complex)|interpro:IPR001233(Protein of unknown function UPF0027)|interpro:IPR027513|interpro:IPR036025|mint:Q99LF4|ensembl:ENSMUST00000001834(transcript)|refseq:NP_663397.1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:ISQNiLQA7DCcgBkTueMDhUHrkFQ10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P62242	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-351820|uniprotkb:P09058|uniprotkb:Q8C2G6|ensembl:ENSMUSP00000099757	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:rs8_mouse(display_long)|uniprotkb:Rps8(gene name)|psi-mi:Rps8(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_033124.1|ensembl:ENSMUSG00000047675(gene)|ensembl:ENSMUST00000102696(transcript)|go:"GO:0000462"("maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)")|go:"GO:0003735"(structural constituent of ribosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005829"(cytosol)|go:"GO:0006412"(translation)|go:"GO:0016020"(membrane)|go:"GO:0022626"(cytosolic ribosome)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:1990904"(ribonucleoprotein complex)|interpro:IPR001047(Ribosomal protein S8e)|interpro:IPR018283|interpro:IPR022309|interpro:IPR042563|mint:P62242|reactome:R-MMU-156827|reactome:R-MMU-1799339|reactome:R-MMU-6791226|reactome:R-MMU-72649|reactome:R-MMU-72689|reactome:R-MMU-72695|reactome:R-MMU-72702|reactome:R-MMU-72706|reactome:R-MMU-975956|reactome:R-MMU-975957	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:3rpCBdQyRWR+TCKnupbnCp30jKE10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P62754	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-2000022|uniprotkb:P08227|uniprotkb:P10660|ensembl:ENSMUSP00000099878	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:rs6_mouse(display_long)|uniprotkb:Rps6(gene name)|psi-mi:Rps6(display_short)|uniprotkb:Phosphoprotein NP33(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_033122.1|refseq:XP_011238179.1|ensembl:ENSMUSG00000028495(gene)|ensembl:ENSMUST00000102814(transcript)|go:"GO:0000082"(G1/S transition of mitotic cell cycle)|go:"GO:0000278"(mitotic cell cycle)|go:"GO:0001890"(placenta development)|go:"GO:0002309"(T cell proliferation involved in immune response)|go:"GO:0003729"(mRNA binding)|go:"GO:0003735"(structural constituent of ribosome)|go:"GO:0005634"(nucleus)|go:"GO:0005730"(nucleolus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005829"(cytosol)|go:"GO:0005840"(ribosome)|go:"GO:0005844"(polysome)|go:"GO:0006364"(rRNA processing)|go:"GO:0006412"(translation)|go:"GO:0006924"(activation-induced cell death of T cells)|go:"GO:0007369"(gastrulation)|go:"GO:0008284"(positive regulation of cell population proliferation)|go:"GO:0015935"(small ribosomal subunit)|interpro:IPR018282|mint:P62754|reactome:R-MMU-156827|go:"GO:0019901"(protein kinase binding)|go:"GO:0022605"(mammalian oogenesis stage)|go:"GO:0022626"(cytosolic ribosome)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0030425"(dendrite)|go:"GO:0031929"(TOR signaling)|go:"GO:0033077"(T cell differentiation in thymus)|go:"GO:0036464"(cytoplasmic ribonucleoprotein granule)|go:"GO:0042274"(ribosomal small subunit biogenesis)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0044297"(cell body)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0048821"(erythrocyte development)|go:"GO:1990904"(ribonucleoprotein complex)|interpro:IPR001377(Ribosomal protein S6e)|interpro:IPR014401(Ribosomal protein S6, eukaryotic)|dip:DIP-42772N|reactome:R-MMU-166208|reactome:R-MMU-1799339|reactome:R-MMU-6791226|reactome:R-MMU-72649|reactome:R-MMU-72689|reactome:R-MMU-72695|reactome:R-MMU-72702|reactome:R-MMU-72706|reactome:R-MMU-975956|reactome:R-MMU-975957	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:beDuXCIqL99V9JlVfpzx7MdpH6810090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9DBG6	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-529941|uniprotkb:Q3TKY0|uniprotkb:Q91XH0|uniprotkb:A2ACG6|ensembl:ENSMUSP00000112081	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:rpn2_mouse(display_long)|uniprotkb:Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit(gene name synonym)|uniprotkb:Ribophorin-2(gene name synonym)|uniprotkb:Ribophorin II(gene name synonym)|uniprotkb:Rpn2(gene name)|psi-mi:Rpn2(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_062616.2|ensembl:ENSMUSG00000027642(gene)|ensembl:ENSMUST00000116380(transcript)|go:"GO:0000421"(autophagosome membrane)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005791"(rough endoplasmic reticulum)|go:"GO:0006487"(protein N-linked glycosylation)|go:"GO:0007568"(aging)|go:"GO:0008250"(oligosaccharyltransferase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016604"(nuclear body)|go:"GO:0042493"|go:"GO:0043022"(ribosome binding)|interpro:IPR008814(Ribophorin II)|mint:Q9DBG6|rcsb pdb:5J6G|rcsb pdb:5J6H	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:FeAH2GGWVY8J5dSaAvfrsBSx3co10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P12970	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-773955|uniprotkb:Q91YM3|ensembl:ENSMUSP00000099962	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:rl7a_mouse(display_long)|uniprotkb:Surfeit locus protein 3(gene name synonym)|uniprotkb:Rpl7a(gene name)|psi-mi:Rpl7a(display_short)|uniprotkb:Surf3(gene name synonym)|uniprotkb:Surf-3(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_038749.1|ensembl:ENSMUSG00000062647(gene)|ensembl:ENSMUST00000102898(transcript)|go:"GO:0000470"(maturation of LSU-rRNA)|go:"GO:0003723"(RNA binding)|go:"GO:0003735"(structural constituent of ribosome)|go:"GO:0005730"(nucleolus)|go:"GO:0005829"(cytosol)|go:"GO:0005840"(ribosome)|go:"GO:0016020"(membrane)|go:"GO:0022625"(cytosolic large ribosomal subunit)|go:"GO:0022626"(cytosolic ribosome)|go:"GO:0042788"(polysomal ribosome)|go:"GO:0045202"(synapse)|interpro:IPR001921(Ribosomal protein L7A)|interpro:IPR004037(Ribosomal protein L7Ae)|interpro:IPR004038(Ribosomal protein L7Ae/L30e/S12e/Gadd45)|interpro:IPR018492|interpro:IPR029064|mint:P12970|rcsb pdb:6SWA|reactome:R-MMU-156827|reactome:R-MMU-1799339|reactome:R-MMU-6791226|reactome:R-MMU-72689|reactome:R-MMU-72706|reactome:R-MMU-975956|reactome:R-MMU-975957	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:XRP8YT3AaHxiv41NmJygfWNtBEs10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9CPR4	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-6260896	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:rl17_mouse(display_long)|uniprotkb:Rpl17(gene name)|psi-mi:Rpl17(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	go:"GO:0002181"(cytoplasmic translation)|go:"GO:0003735"(structural constituent of ribosome)|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005844"(polysome)|go:"GO:0022625"(cytosolic large ribosomal subunit)|go:"GO:0022626"(cytosolic ribosome)|go:"GO:0031672"(A band)|go:"GO:0045202"(synapse)|go:"GO:0070180"(large ribosomal subunit rRNA binding)|go:"GO:1900087"(positive regulation of G1/S transition of mitotic cell cycle)|reactome:R-MMU-156827|reactome:R-MMU-1799339|reactome:R-MMU-6791226|reactome:R-MMU-72689|reactome:R-MMU-72706|reactome:R-MMU-975956|reactome:R-MMU-975957|rcsb pdb:6SWA|mint:Q9CPR4|interpro:IPR001063(Ribosomal protein L22/L17)|interpro:IPR005721(Ribosomal protein L22/L17, eukaryotic/archaeal)|interpro:IPR018260|interpro:IPR036394	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:1O/Lf4KUmT/kaME3XavS27J+PGs10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P70336	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-771800|uniprotkb:Q8BR64|uniprotkb:Q8CBR0|uniprotkb:A5XDA7	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:rock2_mouse(display_long)|uniprotkb:Rho-associated, coiled-coil-containing protein kinase 2(gene name synonym)|uniprotkb:p164 ROCK-2(gene name synonym)|uniprotkb:Rho-associated, coiled-coil-containing protein kinase II(gene name synonym)|uniprotkb:Rock2(gene name)|psi-mi:Rock2(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_033098.2|interpro:IPR000961(Protein kinase, C-terminal)|interpro:IPR011009(Protein kinase-like)|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR001849(Pleckstrin-like)|interpro:IPR002219(Protein kinase C, phorbol ester/diacylglycerol binding)|interpro:IPR000719(Protein kinase, core)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020684|interpro:IPR015008(Rho Binding)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011072(HR1 rho-binding repeat)|mint:P70336|interpro:IPR037311|go:"GO:0000281"(mitotic cytokinesis)|go:"GO:0001837"(epithelial to mesenchymal transition)|go:"GO:0001843"(neural tube closure)|go:"GO:0001934"(positive regulation of protein phosphorylation)|go:"GO:0002931"(response to ischemia)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005813"(centrosome)|go:"GO:0005829"(cytosol)|go:"GO:0005856"(cytoskeleton)|go:"GO:0005886"(plasma membrane)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006939"(smooth muscle contraction)|go:"GO:0007249"(I-kappaB kinase/NF-kappaB signaling)|go:"GO:0007266"(Rho protein signal transduction)|go:"GO:0008625"(extrinsic apoptotic signaling pathway via death domain receptors)|go:"GO:0010595"(positive regulation of endothelial cell migration)|go:"GO:0010613"(positive regulation of cardiac muscle hypertrophy)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0010629"(negative regulation of gene expression)|go:"GO:0010825"(positive regulation of centrosome duplication)|go:"GO:0016525"(negative regulation of angiogenesis)|go:"GO:0018105"(peptidyl-serine phosphorylation)|go:"GO:0018107"(peptidyl-threonine phosphorylation)|go:"GO:0030036"(actin cytoskeleton organization)|go:"GO:0030335"(positive regulation of cell migration)|go:"GO:0030866"(cortical actin cytoskeleton organization)|go:"GO:0031032"(actomyosin structure organization)|go:"GO:0031267"(small GTPase binding)|go:"GO:0031616"(spindle pole centrosome)|go:"GO:0031644"(regulation of nervous system process)|go:"GO:0032723"(positive regulation of connective tissue growth factor production)|go:"GO:0035509"(negative regulation of myosin-light-chain-phosphatase activity)|go:"GO:0036464"(cytoplasmic ribonucleoprotein granule)|go:"GO:0039694"(viral RNA genome replication)|go:"GO:0042752"(regulation of circadian rhythm)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045019"(negative regulation of nitric oxide biosynthetic process)|go:"GO:0045616"(regulation of keratinocyte differentiation)|go:"GO:0046872"(metal ion binding)|go:"GO:0048511"(rhythmic process)|go:"GO:0048598"(embryonic morphogenesis)|go:"GO:0048813"(dendrite morphogenesis)|go:"GO:0051246"(regulation of protein metabolic process)|go:"GO:0051298"(centrosome duplication)|go:"GO:0051496"(positive regulation of stress fiber assembly)|go:"GO:0061157"(mRNA destabilization)|go:"GO:0071394"(cellular response to testosterone stimulus)|go:"GO:0071559"(response to transforming growth factor beta)|go:"GO:0072518"(Rho-dependent protein serine/threonine kinase activity)|go:"GO:0072659"(protein localization to plasma membrane)|go:"GO:0090271"(positive regulation of fibroblast growth factor production)|go:"GO:0097746"(blood vessel diameter maintenance)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098974"(postsynaptic actin cytoskeleton organization)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:0110061"(regulation of angiotensin-activated signaling pathway)|go:"GO:0150033"(negative regulation of protein localization to lysosome)|go:"GO:1900037"(regulation of cellular response to hypoxia)|go:"GO:1901888"(regulation of cell junction assembly)|go:"GO:1902004"(positive regulation of amyloid-beta formation)|go:"GO:1902961"(positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process)|go:"GO:1902966"(positive regulation of protein localization to early endosome)|go:"GO:1902993"(positive regulation of amyloid precursor protein catabolic process)|go:"GO:1903140"(regulation of establishment of endothelial barrier)|go:"GO:1903347"(negative regulation of bicellular tight junction assembly)|go:"GO:1905145"(cellular response to acetylcholine)|go:"GO:1905205"(positive regulation of connective tissue replacement)|go:"GO:1990776"(response to angiotensin)|reactome:R-MMU-3928662|reactome:R-MMU-416482|reactome:R-MMU-416572|reactome:R-MMU-4420097|reactome:R-MMU-5627117|reactome:R-MMU-8980692|reactome:R-MMU-9013026|reactome:R-MMU-9013106|reactome:R-MMU-9013407|reactome:R-MMU-9013422	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:XGh4GUDWts/RI6osd4zKXeh0lAE10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P60764	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-644949|uniprotkb:O14658|ensembl:ENSMUSP00000018156	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:rac3_mouse(display_long)|uniprotkb:p21-Rac3(gene name synonym)|uniprotkb:Rac3(gene name)|psi-mi:Rac3(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_573486.1|go:"GO:0007163"(establishment or maintenance of cell polarity)|go:"GO:0007264"(small GTPase mediated signal transduction)|ensembl:ENSMUSG00000018012(gene)|go:"GO:0008360"(regulation of cell shape)|go:"GO:0012505"(endomembrane system)|go:"GO:0014041"(regulation of neuron maturation)|go:"GO:0016055"(Wnt signaling pathway)|go:"GO:0019901"(protein kinase binding)|go:"GO:0021894"(cerebral cortex GABAergic interneuron development)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030027"(lamellipodium)|go:"GO:0030031"(cell projection assembly)|go:"GO:0030036"(actin cytoskeleton organization)|go:"GO:0030426"(growth cone)|go:"GO:0030865"(cortical cytoskeleton organization)|go:"GO:0031175"(neuron projection development)|ensembl:ENSMUST00000018156(transcript)|go:"GO:0003924"(GTPase activity)|go:"GO:0003925"(G protein activity)|go:"GO:0005525"(GTP binding)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0005829"(cytosol)|go:"GO:0005856"(cytoskeleton)|go:"GO:0005886"(plasma membrane)|go:"GO:0005938"(cell cortex)|go:"GO:0007015"(actin filament organization)|go:"GO:0031941"(filamentous actin)|go:"GO:0032956"(regulation of actin cytoskeleton organization)|go:"GO:0033630"(positive regulation of cell adhesion mediated by integrin)|go:"GO:0042995"(cell projection)|go:"GO:0043005"(neuron projection)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0045202"(synapse)|go:"GO:0048306"(calcium-dependent protein binding)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0048873"(homeostasis of number of cells within a tissue)|go:"GO:0050885"(neuromuscular process controlling balance)|go:"GO:0050905"(neuromuscular process)|go:"GO:0051932"(synaptic transmission, GABAergic)|go:"GO:0071944"(cell periphery)|go:"GO:1900026"(positive regulation of substrate adhesion-dependent cell spreading)|interpro:IPR001806(Ras GTPase)|interpro:IPR003578(Ras small GTPase, Rho type)|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR027417|mint:P60764|reactome:R-MMU-4086400|reactome:R-MMU-9013423	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:YWu9Tmqxzxu49RSwmyfhawoOUis10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:O09000	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-3508338|uniprotkb:Q9CRD5	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:ncoa3_mouse(display_long)|uniprotkb:Ncoa3(gene name)|psi-mi:Ncoa3(display_short)|uniprotkb:Aib1(gene name synonym)|uniprotkb:Pcip(gene name synonym)|uniprotkb:Rac3(gene name synonym)|uniprotkb:Tram1(gene name synonym)|uniprotkb:Amplified in breast cancer-1 protein homolog(gene name synonym)|uniprotkb:CBP-interacting protein(gene name synonym)|uniprotkb:Receptor-associated coactivator 3(gene name synonym)|uniprotkb:Steroid receptor coactivator protein 3(gene name synonym)|uniprotkb:Thyroid hormone receptor activator molecule 1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	go:"GO:2000036"(regulation of stem cell population maintenance)|go:"GO:2000737"(negative regulation of stem cell differentiation)|interpro:IPR000014(PAS)|interpro:IPR009110(Nuclear receptor coactivator, interlocking)|interpro:IPR010011(Protein of unknown function DUF1518)|interpro:IPR011598(Helix-loop-helix DNA-binding)|interpro:IPR013767(PAS fold)|interpro:IPR014920(Nuclear receptor coactivator, Ncoa-type, interlocking)|interpro:IPR014935(Steroid receptor coactivator)|interpro:IPR017426(Nuclear receptor coactivator)|interpro:IPR028818|interpro:IPR032565|interpro:IPR035965|interpro:IPR036638|interpro:IPR037077|reactome:R-MMU-5687128|reactome:R-MMU-9018519|go:"GO:0090575"(RNA polymerase II transcription regulator complex)|go:"GO:0097718"(disordered domain specific binding)|go:"GO:1902459"(positive regulation of stem cell population maintenance)|go:"GO:2000035"(regulation of stem cell division)|go:"GO:0000785"(chromatin)|go:"GO:0000977"(RNA polymerase II transcription regulatory region sequence-specific DNA binding)|go:"GO:0000993"(RNA polymerase II complex binding)|go:"GO:0001228"(DNA-binding transcription activator activity, RNA polymerase II-specific)|go:"GO:0003682"(chromatin binding)|go:"GO:0003713"(transcription coactivator activity)|go:"GO:0004402"(histone acetyltransferase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0016922"(nuclear receptor binding)|go:"GO:0030307"(positive regulation of cell growth)|go:"GO:0032870"(cellular response to hormone stimulus)|go:"GO:0032991"(protein-containing complex)|go:"GO:0033145"(positive regulation of intracellular steroid hormone receptor signaling pathway)|go:"GO:0033148"(positive regulation of intracellular estrogen receptor signaling pathway)|go:"GO:0030331"(estrogen receptor binding)|go:"GO:0035264"(multicellular organism growth)|go:"GO:0042975"(peroxisome proliferator activated receptor binding)|go:"GO:0030374"(nuclear receptor coactivator activity)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0043697"(cell dedifferentiation)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045618"(positive regulation of keratinocyte differentiation)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046983"(protein dimerization activity)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0048589"(developmental growth)|go:"GO:0050681"(androgen receptor binding)|go:"GO:0051091"(positive regulation of DNA-binding transcription factor activity)|go:"GO:0060068"(vagina development)|go:"GO:0060713"(labyrinthine layer morphogenesis)|go:"GO:0060744"(mammary gland branching involved in thelarche)|go:"GO:0071392"(cellular response to estradiol stimulus)|go:"GO:0072091"(regulation of stem cell proliferation)|dip:DIP-44921N|mint:O09000	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:kUBr4kzfOKOmyqLPHkFpp4vQQ5s10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P61294	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-529766|ensembl:ENSMUSP00000035155	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:rab6b_mouse(display_long)|uniprotkb:Rab6b(gene name)|psi-mi:Rab6b(display_short)|uniprotkb:D9Bwg0185e(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_776142.1|ensembl:ENSMUSG00000032549(gene)|ensembl:ENSMUST00000035155(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005793"(endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006890"(retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0012505"(endomembrane system)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031489"(myosin V binding)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0098793"(presynapse)|interpro:IPR001806(Ras GTPase)|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR027417|mint:P61294|reactome:R-MMU-6811436|reactome:R-MMU-6811440|reactome:R-MMU-8854214|reactome:R-MMU-8873719|reactome:R-MMU-8876198	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:GmhjkLknjr8fxoUBxNx5ak5S/hQ10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q8CHD8	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-26595972|ensembl:ENSMUSP00000112875|uniprotkb:Q8JZT3	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:rfip3_mouse(display_long)|uniprotkb:Rab11fip3(gene name)|psi-mi:Rab11fip3(display_short)|uniprotkb:Kiaa0665(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000037098(gene)|ensembl:ENSMUST00000120691(transcript)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005768"(endosome)|go:"GO:0005813"(centrosome)|go:"GO:0007049"(cell cycle)|go:"GO:0016020"(membrane)|go:"GO:0030139"(endocytic vesicle)|go:"GO:0030496"(midbody)|go:"GO:0031267"(small GTPase binding)|go:"GO:0032154"(cleavage furrow)|go:"GO:0032456"(endocytic recycling)|go:"GO:0032465"(regulation of cytokinesis)|go:"GO:0034451"(centriolar satellite)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0045171"(intercellular bridge)|go:"GO:0051301"(cell division)|go:"GO:0051959"(dynein light intermediate chain binding)|go:"GO:0055037"(recycling endosome)|go:"GO:0055038"(recycling endosome membrane)|go:"GO:0061512"(protein localization to cilium)|go:"GO:0070164"(negative regulation of adiponectin secretion)|interpro:IPR002048(Calcium-binding EF-hand)|interpro:IPR011992(EF-Hand type)|interpro:IPR019018|interpro:IPR032920|interpro:IPR037245|mint:Q8CHD8|reactome:R-MMU-5620916|refseq:NP_001156340.1|refseq:NP_001156341.1|refseq:NP_694780.1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:tg/aR/dVRoVe8kLZY28hzDX/rO010090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:B9EKR1	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-15946373|ensembl:ENSMUSP00000088056	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:ptprz_mouse(display_long)|uniprotkb:Ptprz1(gene name)|psi-mi:Ptprz1(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUST00000090568(transcript)|go:"GO:0002244"(hematopoietic progenitor cell differentiation)|go:"GO:0004725"(protein tyrosine phosphatase activity)|go:"GO:0005178"(integrin binding)|go:"GO:0005615"(extracellular space)|go:"GO:0005737"(cytoplasm)|go:"GO:0005886"(plasma membrane)|go:"GO:0006470"(protein dephosphorylation)|go:"GO:0007409"(axonogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007611"(learning or memory)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010812"(negative regulation of cell-substrate adhesion)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0016020"(membrane)|go:"GO:0016791"(phosphatase activity)|go:"GO:0017134"(fibroblast growth factor binding)|ensembl:ENSMUSG00000068748(gene)|go:"GO:0030027"(lamellipodium)|go:"GO:0030175"(filopodium)|go:"GO:0030335"(positive regulation of cell migration)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0030426"(growth cone)|go:"GO:0031012"(extracellular matrix)|go:"GO:0031226"(intrinsic component of plasma membrane)|go:"GO:0031641"(regulation of myelination)|go:"GO:0032587"(ruffle membrane)|go:"GO:0035335"(peptidyl-tyrosine dephosphorylation)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043197"(dendritic spine)|go:"GO:0043524"(negative regulation of neuron apoptotic process)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0048146"(positive regulation of fibroblast proliferation)|go:"GO:0048709"(oligodendrocyte differentiation)|go:"GO:0048714"(positive regulation of oligodendrocyte differentiation)|go:"GO:0048814"(regulation of dendrite morphogenesis)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0070445"(regulation of oligodendrocyte progenitor proliferation)|go:"GO:0072534"(perineuronal net)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099061"(integral component of postsynaptic density membrane)|go:"GO:1900006"(positive regulation of dendrite development)|go:"GO:1900149"(positive regulation of Schwann cell migration)|go:"GO:1901216"(positive regulation of neuron death)|go:"GO:2000171"(negative regulation of dendrite development)|go:"GO:2001224"(positive regulation of neuron migration)|interpro:IPR000242(Protein-tyrosine phosphatase, receptor/non-receptor type)|interpro:IPR000387(Protein-tyrosine phosphatase)|interpro:IPR001148(Carbonic anhydrase, eukaryotic)|interpro:IPR003595(Protein-tyrosine phosphatase, catalytic)|interpro:IPR003961(Fibronectin, type III)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR016130(Protein-tyrosine phosphatase, active site)|interpro:IPR029021|interpro:IPR036116|interpro:IPR036398|interpro:IPR041887|mint:B9EKR1|reactome:R-MMU-449836|refseq:NP_001074775.1	-	-	crc64:91C5D58F74BA999C	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:77BTIhylf3r1wcOe95cD5NHguRg10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:B0V2N1	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-647877|uniprotkb:Q3TEC3|uniprotkb:Q3TXC9|uniprotkb:Q5XJV4|uniprotkb:Q64503|uniprotkb:Q64699|uniprotkb:Q7TT17|uniprotkb:Q4JFC7|ensembl:ENSMUSP00000064048	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:ptprs_mouse(display_long)|uniprotkb:Ptprs(gene name)|psi-mi:Ptprs(display_short)|uniprotkb:Receptor-type tyrosine-protein phosphatase sigma(gene name synonym)|uniprotkb:PTPNU-3(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_035348.2|refseq:NP_001239382.1|refseq:NP_001239384.1|refseq:NP_001239385.1|go:"GO:0021549"(cerebellum development)|go:"GO:0021766"(hippocampus development)|go:"GO:0021987"(cerebral cortex development)|go:"GO:0022038"(corpus callosum development)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030424"(axon)|go:"GO:0030426"(growth cone)|go:"GO:0030517"(negative regulation of axon extension)|go:"GO:0032687"(negative regulation of interferon-alpha production)|go:"GO:0032688"(negative regulation of interferon-beta production)|go:"GO:0034164"(negative regulation of toll-like receptor 9 signaling pathway)|go:"GO:0035335"(peptidyl-tyrosine dephosphorylation)|go:"GO:0035374"(chondroitin sulfate binding)|go:"GO:0043204"(perikaryon)|go:"GO:0043395"(heparan sulfate proteoglycan binding)|go:"GO:0048671"(negative regulation of collateral sprouting)|go:"GO:0048681"(negative regulation of axon regeneration)|go:"GO:0050804"(modulation of chemical synaptic transmission)|go:"GO:0050808"(synapse organization)|go:"GO:0061000"(negative regulation of dendritic spine development)|go:"GO:0090557"(establishment of endothelial intestinal barrier)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|go:"GO:0099061"(integral component of postsynaptic density membrane)|go:"GO:0099151"(regulation of postsynaptic density assembly)|go:"GO:0099560"(synaptic membrane adhesion)|go:"GO:1905606"(regulation of presynapse assembly)|interpro:IPR000242(Protein-tyrosine phosphatase, receptor/non-receptor type)|interpro:IPR000387(Protein-tyrosine phosphatase)|interpro:IPR003595(Protein-tyrosine phosphatase, catalytic)|interpro:IPR003598(Immunoglobulin subtype 2)|interpro:IPR003599(Immunoglobulin subtype)|interpro:IPR003961(Fibronectin, type III)|interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013098(Immunoglobulin I-set)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR016130(Protein-tyrosine phosphatase, active site)|interpro:IPR029021|interpro:IPR036116|interpro:IPR036179|mint:B0V2N1|rcsb pdb:3SR9|reactome:R-MMU-388844|reactome:R-MMU-8849932|ensembl:ENSMUSG00000013236(gene)|ensembl:ENSMUST00000067538(transcript)|go:"GO:0004721"(phosphoprotein phosphatase activity)|go:"GO:0004725"(protein tyrosine phosphatase activity)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006470"(protein dephosphorylation)|go:"GO:0008201"(heparin binding)|go:"GO:0010977"(negative regulation of neuron projection development)|go:"GO:0021510"(spinal cord development)	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:ZHccWWvEOOQcSSD7ic6ow6xeqtU10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9QVP9	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-299061|ensembl:ENSMUSP00000022622|ensembl:ENSMUSP00000137008|uniprotkb:B2RQ16|uniprotkb:G3X8V1	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:fak2_mouse(display_long)|uniprotkb:Pyk2(gene name synonym)|uniprotkb:Raftk(gene name synonym)|uniprotkb:Ptk2b(gene name)|psi-mi:Ptk2b(display_short)|uniprotkb:Fak2(gene name synonym)|uniprotkb:Focal adhesion kinase 2(gene name synonym)|uniprotkb:Proline-rich tyrosine kinase 2(gene name synonym)|uniprotkb:Cell adhesion kinase beta(gene name synonym)|uniprotkb:Calcium-dependent tyrosine kinase(gene name synonym)|uniprotkb:Related adhesion focal tyrosine kinase(gene name synonym)|uniprotkb:Calcium-regulated non-receptor proline-rich tyrosine kinase(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000059456(gene)|ensembl:ENSMUST00000022622(transcript)|ensembl:ENSMUST00000178730(transcript)|go:"GO:0000165"(MAPK cascade)|go:"GO:0001525"(angiogenesis)|go:"GO:0001556"(oocyte maturation)|go:"GO:0001666"(response to hypoxia)|go:"GO:0001954"(positive regulation of cell-matrix adhesion)|go:"GO:0002040"(sprouting angiogenesis)|go:"GO:0002250"(adaptive immune response)|go:"GO:0002315"(marginal zone B cell differentiation)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004683"(calmodulin-dependent protein kinase activity)|go:"GO:0004713"(protein tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0004715"(non-membrane spanning protein tyrosine kinase activity)|go:"GO:0004972"(NMDA glutamate receptor activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005856"(cytoskeleton)|go:"GO:0005925"(focal adhesion)|go:"GO:0005938"(cell cortex)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006968"(cellular defense response)|go:"GO:0006970"(response to osmotic stress)|go:"GO:0007015"(actin filament organization)|go:"GO:0007155"(cell adhesion)|go:"GO:0007166"(cell surface receptor signaling pathway)|go:"GO:0007169"(transmembrane receptor protein tyrosine kinase signaling pathway)|go:"GO:0007172"(signal complex assembly)|go:"GO:0007173"(epidermal growth factor receptor signaling pathway)|go:"GO:0007204"(positive regulation of cytosolic calcium ion concentration)|go:"GO:0007229"(integrin-mediated signaling pathway)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0008284"(positive regulation of cell population proliferation)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0008360"(regulation of cell shape)|go:"GO:0009612"(response to mechanical stimulus)|go:"GO:0009725"(response to hormone)|go:"GO:0009749"(response to glucose)|go:"GO:0010226"(response to lithium ion)|go:"GO:0010595"(positive regulation of endothelial cell migration)|go:"GO:0030425"(dendrite)|go:"GO:0030426"(growth cone)|go:"GO:0030502"(negative regulation of bone mineralization)|go:"GO:0030838"(positive regulation of actin filament polymerization)|go:"GO:0031175"(neuron projection development)|go:"GO:0010656"(negative regulation of muscle cell apoptotic process)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0010752"(regulation of cGMP-mediated signaling)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0010758"(regulation of macrophage chemotaxis)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014009"(glial cell proliferation)|go:"GO:0014069"(postsynaptic density)|go:"GO:0017146"(NMDA selective glutamate receptor complex)|go:"GO:0018108"(peptidyl-tyrosine phosphorylation)|go:"GO:0019899"(enzyme binding)|go:"GO:0030027"(lamellipodium)|go:"GO:0030154"(cell differentiation)|go:"GO:0030155"(regulation of cell adhesion)|go:"GO:0030279"(negative regulation of ossification)|go:"GO:0030307"(positive regulation of cell growth)|go:"GO:0030335"(positive regulation of cell migration)|go:"GO:0030424"(axon)|go:"GO:0032270"(positive regulation of cellular protein metabolic process)|go:"GO:0032960"(regulation of inositol trisphosphate biosynthetic process)|go:"GO:0033209"(tumor necrosis factor-mediated signaling pathway)|go:"GO:0035235"(ionotropic glutamate receptor signaling pathway)|go:"GO:0035902"(response to immobilization stress)|go:"GO:0038083"(peptidyl-tyrosine autophosphorylation)|go:"GO:0042220"(response to cocaine)|go:"GO:0042493"|go:"GO:0042542"(response to hydrogen peroxide)|go:"GO:0042976"(activation of Janus kinase activity)|go:"GO:0042995"(cell projection)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0043149"(stress fiber assembly)|go:"GO:0043197"(dendritic spine)|go:"GO:0043267"(negative regulation of potassium ion transport)|go:"GO:0043423"(3-phosphoinositide-dependent protein kinase binding)|go:"GO:0043507"(positive regulation of JUN kinase activity)|go:"GO:0043524"(negative regulation of neuron apoptotic process)|go:"GO:0043534"(blood vessel endothelial cell migration)|go:"GO:0043552"(positive regulation of phosphatidylinositol 3-kinase activity)|go:"GO:0044297"(cell body)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045087"(innate immune response)|go:"GO:0045121"(membrane raft)|go:"GO:0045428"(regulation of nitric oxide biosynthetic process)|go:"GO:0045429"(positive regulation of nitric oxide biosynthetic process)|go:"GO:0045453"(bone resorption)|go:"GO:2000114"(regulation of establishment of cell polarity)|go:"GO:0045471"(response to ethanol)|go:"GO:0045638"(negative regulation of myeloid cell differentiation)|go:"GO:0045727"(positive regulation of translation)|go:"GO:0045766"(positive regulation of angiogenesis)|go:"GO:0045860"(positive regulation of protein kinase activity)|go:"GO:0046330"(positive regulation of JNK cascade)|go:"GO:0048010"(vascular endothelial growth factor receptor signaling pathway)|go:"GO:0048041"(focal adhesion assembly)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050848"(regulation of calcium-mediated signaling)|go:"GO:0051000"(positive regulation of nitric-oxide synthase activity)|go:"GO:0051279"(regulation of release of sequestered calcium ion into cytosol)|go:"GO:0051591"(response to cAMP)|go:"GO:0051592"(response to calcium ion)|go:"GO:0051968"(positive regulation of synaptic transmission, glutamatergic)|go:"GO:0060291"(long-term synaptic potentiation)|go:"GO:0060292"(long-term synaptic depression)|go:"GO:0070098"(chemokine-mediated signaling pathway)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071300"(cellular response to retinoic acid)|go:"GO:0071498"(cellular response to fluid shear stress)|go:"GO:0086100"(endothelin receptor signaling pathway)|go:"GO:0090630"(activation of GTPase activity)|go:"GO:0097440"(apical dendrite)|go:"GO:0098794"(postsynapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:2000058"(regulation of ubiquitin-dependent protein catabolic process)|go:"GO:2000060"(positive regulation of ubiquitin-dependent protein catabolic process)|go:"GO:2000249"(regulation of actin cytoskeleton reorganization)|go:"GO:2000310"(regulation of NMDA receptor activity)|go:"GO:2000379"(positive regulation of reactive oxygen species metabolic process)|go:"GO:2000463"(positive regulation of excitatory postsynaptic potential)|go:"GO:2000538"(positive regulation of B cell chemotaxis)|go:"GO:2000573"(positive regulation of DNA biosynthetic process)|interpro:IPR000299(Band 4.1, N-terminal)|interpro:IPR000719(Protein kinase, core)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR005189(Focal adhesion targeting region)|interpro:IPR008266(Tyrosine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR014352(FERM/acyl-CoA-binding protein, 3-helical bundle)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR019748|interpro:IPR019749|interpro:IPR020635|interpro:IPR029071|interpro:IPR030610|interpro:IPR035963|interpro:IPR036137|interpro:IPR041390|interpro:IPR041784|mint:Q9QVP9|reactome:R-MMU-391160|reactome:R-MMU-4420097|reactome:R-MMU-9020558|refseq:XP_006518789.1|refseq:XP_011243296.1|refseq:NP_001155838.1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:wFolkPm20C8WJ9CP/rnhc2FxqT410090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q3TXS7	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-991976|uniprotkb:B2RRP8|ensembl:ENSMUSP00000027432	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:psmd1_mouse(display_long)|uniprotkb:26S proteasome regulatory subunit RPN2(gene name synonym)|uniprotkb:26S proteasome regulatory subunit S1(gene name synonym)|uniprotkb:Psmd1(gene name)|psi-mi:Psmd1(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_081633.1|ensembl:ENSMUSG00000026229(gene)|ensembl:ENSMUST00000027432(transcript)|go:"GO:0000502"(proteasome complex)|go:"GO:0005634"(nucleus)|go:"GO:0008540"(proteasome regulatory particle, base subcomplex)|go:"GO:0022624"(proteasome accessory complex)|go:"GO:0030234"(enzyme regulator activity)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0034515"(proteasome storage granule)|go:"GO:0042176"(regulation of protein catabolic process)|go:"GO:0043161"(proteasome-mediated ubiquitin-dependent protein catabolic process)|interpro:IPR002015(Proteasome/cyclosome, regulatory subunit)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR016642(26S proteasome regulatory complex, non-ATPase subcomplex, Rpn2/Psmd1 subunit)|interpro:IPR035266|interpro:IPR040623|mint:Q3TXS7|reactome:R-MMU-1169091|reactome:R-MMU-1234176|reactome:R-MMU-1236978|reactome:R-MMU-174084|reactome:R-MMU-174154|reactome:R-MMU-174178|reactome:R-MMU-174184|reactome:R-MMU-187577|reactome:R-MMU-195253|reactome:R-MMU-382556|reactome:R-MMU-450408|reactome:R-MMU-202424|reactome:R-MMU-2467813|reactome:R-MMU-2871837|reactome:R-MMU-349425|reactome:R-MMU-350562|reactome:R-MMU-4608870|reactome:R-MMU-4641257|reactome:R-MMU-4641258|reactome:R-MMU-5358346|reactome:R-MMU-5607761|reactome:R-MMU-5607764|reactome:R-MMU-5610780|reactome:R-MMU-5610785|reactome:R-MMU-5632684|reactome:R-MMU-5658442|reactome:R-MMU-5668541|reactome:R-MMU-5676590|reactome:R-MMU-5687128|reactome:R-MMU-5689603|reactome:R-MMU-5689880|reactome:R-MMU-6798695|reactome:R-MMU-68827|reactome:R-MMU-68949|reactome:R-MMU-69017|reactome:R-MMU-69481|reactome:R-MMU-69601|reactome:R-MMU-75815|reactome:R-MMU-8852276|reactome:R-MMU-8854050|reactome:R-MMU-8932339|reactome:R-MMU-8939236|reactome:R-MMU-8939902|reactome:R-MMU-8941858|reactome:R-MMU-8948751|reactome:R-MMU-8951664|reactome:R-MMU-9020702|reactome:R-MMU-983168	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:sc+oNyx2S9yhdiY4x08P1NBEGtQ10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P68404	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-397048|uniprotkb:F2Z441|uniprotkb:P04410|uniprotkb:P04411|uniprotkb:A0JNZ5|intact:EBI-4304045|ensembl:ENSMUSP00000064812	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:kpcb_mouse(display_long)|uniprotkb:Prkcb(gene name)|psi-mi:Prkcb(display_short)|uniprotkb:Pkcb(gene name synonym)|uniprotkb:Prkcb1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_032881.1|refseq:NP_001303601.1|ensembl:ENSMUSG00000052889(gene)|ensembl:ENSMUST00000064921(transcript)|go:"GO:0002250"(adaptive immune response)|go:"GO:0003682"(chromatin binding)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004697"(protein kinase C activity)|go:"GO:0004698"(calcium-dependent protein kinase C activity)|go:"GO:0005080"(protein kinase C binding)|go:"GO:0005246"(calcium channel regulator activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005813"(centrosome)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006816"(calcium ion transport)|go:"GO:0006874"(cellular calcium ion homeostasis)|go:"GO:0006915"(apoptotic process)|go:"GO:0008091"(spectrin)|go:"GO:0008270"(zinc ion binding)|go:"GO:0010827"(regulation of glucose transmembrane transport)|go:"GO:0010829"(negative regulation of glucose transmembrane transport)|go:"GO:0014059"(regulation of dopamine secretion)|go:"GO:0018105"(peptidyl-serine phosphorylation)|go:"GO:0030374"(nuclear receptor coactivator activity)|go:"GO:0030949"(positive regulation of vascular endothelial growth factor receptor signaling pathway)|go:"GO:0031526"(brush border membrane)|go:"GO:0035403"("histone kinase activity (H3-T6 specific)")|go:"GO:0035408"(histone H3-T6 phosphorylation)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0040008"(regulation of growth)|go:"GO:0042113"(B cell activation)|go:"GO:0042393"(histone binding)|go:"GO:0042493"|go:"GO:0043123"(positive regulation of I-kappaB kinase/NF-kappaB signaling)|go:"GO:0044305"(calyx of Held)|go:"GO:0045766"(positive regulation of angiogenesis)|go:"GO:0046627"(negative regulation of insulin receptor signaling pathway)|go:"GO:0050681"(androgen receptor binding)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050861"(positive regulation of B cell receptor signaling pathway)|go:"GO:0051092"(positive regulation of NF-kappaB transcription factor activity)|go:"GO:0071322"(cellular response to carbohydrate stimulus)|go:"GO:0099171"(presynaptic modulation of chemical synaptic transmission)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR000008(C2 calcium-dependent membrane targeting)|interpro:IPR000719(Protein kinase, core)|interpro:IPR000961(Protein kinase, C-terminal)|interpro:IPR002219(Protein kinase C, phorbol ester/diacylglycerol binding)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR014375(Protein kinase C, alpha/beta/gamma types)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR017892|interpro:IPR020454|interpro:IPR034664|interpro:IPR035892|mint:P68404|reactome:R-MMU-114516|reactome:R-MMU-1169091|reactome:R-MMU-416993|reactome:R-MMU-4419969|reactome:R-MMU-5099900|reactome:R-MMU-5218921|reactome:R-MMU-5668599|reactome:R-MMU-76005|dip:DIP-31583N	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:wPR+19jsOpjLky/X5fpkIK5o5SM10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P31324	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-455340|uniprotkb:Q3UTZ1|uniprotkb:Q80ZM4|uniprotkb:Q8BRZ7|uniprotkb:B1B199|ensembl:ENSMUSP00000003079|ensembl:ENSMUSP00000039797	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:kap3_mouse(display_long)|uniprotkb:Prkar2b(gene name)|psi-mi:Prkar2b(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_035288.2|refseq:XP_006515077.1|dip:DIP-31571N|interpro:IPR003117(cAMP-dependent protein kinase, regulatory subunit, type I/II alpha/beta)|interpro:IPR012198(cAMP-dependent protein kinase regulatory subunit)|interpro:IPR014710(RmlC-like jelly roll fold)|interpro:IPR018488|interpro:IPR018490|mint:P31324|rcsb pdb:3TNP|rcsb pdb:3TNQ|rcsb pdb:4WBB|rcsb pdb:4X6Q|reactome:R-MMU-163615|reactome:R-MMU-164378|reactome:R-MMU-180024|reactome:R-MMU-2565942|reactome:R-MMU-380259|reactome:R-MMU-380270|reactome:R-MMU-380284|reactome:R-MMU-380320|reactome:R-MMU-432040|reactome:R-MMU-442720|reactome:R-MMU-5610787|reactome:R-MMU-5620912|reactome:R-MMU-8854518|reactome:R-MMU-983231|ensembl:ENSMUSG00000002997(gene)|ensembl:ENSMUST00000003079(transcript)|ensembl:ENSMUST00000036497(transcript)|go:"GO:0004862"(cAMP-dependent protein kinase inhibitor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005813"(centrosome)|go:"GO:0005886"(plasma membrane)|go:"GO:0005952"(cAMP-dependent protein kinase complex)|go:"GO:0006631"(fatty acid metabolic process)|go:"GO:0007612"(learning)|go:"GO:0008603"(cAMP-dependent protein kinase regulator activity)|go:"GO:0010738"(regulation of protein kinase A signaling)|go:"GO:0019901"(protein kinase binding)|go:"GO:0019904"(protein domain specific binding)|go:"GO:0030425"(dendrite)|go:"GO:0030552"(cAMP binding)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0034236"(protein kinase A catalytic subunit binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043197"(dendritic spine)|go:"GO:0043198"(dendritic shaft)|go:"GO:0043949"(regulation of cAMP-mediated signaling)|go:"GO:0045121"(membrane raft)|go:"GO:0045859"(regulation of protein kinase activity)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050804"(modulation of chemical synaptic transmission)|go:"GO:0097332"(response to antipsychotic drug)|go:"GO:0097546"(ciliary base)|go:"GO:0098794"(postsynapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0097338"(response to clozapine)|go:"GO:2000480"(negative regulation of cAMP-dependent protein kinase activity)|interpro:IPR000595(Cyclic nucleotide-binding)	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:/d/0NSAnglWjmZAcQUn6ATpbJnE10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P68181	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-400685|uniprotkb:P05206|uniprotkb:Q3TQH5|uniprotkb:Q3UDD0|uniprotkb:Q3UTH5|ensembl:ENSMUSP00000099573	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:kapcb_mouse(display_long)|uniprotkb:Prkacb(gene name)|psi-mi:Prkacb(display_short)|uniprotkb:Pkacb(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001157670.1|refseq:NP_001157671.1|refseq:NP_001157672.1|refseq:NP_035230.1|ensembl:ENSMUSG00000005034(gene)|ensembl:ENSMUST00000102515(transcript)|go:"GO:0000287"(magnesium ion binding)|go:"GO:0001843"(neural tube closure)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004679"(AMP-activated protein kinase activity)|go:"GO:0004691"(cAMP-dependent protein kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005813"(centrosome)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005952"(cAMP-dependent protein kinase complex)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0010737"(protein kinase A signaling)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0051447"(negative regulation of meiotic cell cycle)|go:"GO:0070613"(regulation of protein processing)|go:"GO:0097338"(response to clozapine)|go:"GO:0097546"(ciliary base)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1901621"(negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning)|interpro:IPR000719(Protein kinase, core)|interpro:IPR000961(Protein kinase, C-terminal)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR044109|mint:P68181|reactome:R-MMU-163615|reactome:R-MMU-164378|reactome:R-MMU-180024|reactome:R-MMU-381676|reactome:R-MMU-392517|reactome:R-MMU-422356|reactome:R-MMU-432040|reactome:R-MMU-4420097|reactome:R-MMU-442720|reactome:R-MMU-5610785|reactome:R-MMU-5610787|reactome:R-MMU-5621575|reactome:R-MMU-5687128|reactome:R-MMU-8853659|reactome:R-MMU-8963896|reactome:R-MMU-983231	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:9278c8xZsjU90+vryXpzUc0y/G010090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P99029	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-2735704|uniprotkb:Q9QX45|uniprotkb:Q9QZ75|ensembl:ENSMUSP00000025904	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:prdx5_mouse(display_long)|uniprotkb:Prdx5(gene name)|psi-mi:Prdx5(display_short)|uniprotkb:Prdx6(gene name synonym)|uniprotkb:Peroxiredoxin V(gene name synonym)|uniprotkb:Peroxisomal antioxidant enzyme(gene name synonym)|uniprotkb:PLP(gene name synonym)|uniprotkb:Thioredoxin peroxidase PMP20(gene name synonym)|uniprotkb:Antioxidant enzyme B166(gene name synonym)|uniprotkb:Liver tissue 2D-page spot 2D-0014IV(gene name synonym)|uniprotkb:Thioredoxin-dependent peroxiredoxin 5(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_036151.1|ensembl:ENSMUSG00000024953(gene)|ensembl:ENSMUST00000025904(transcript)|go:"GO:0001016"(RNA polymerase III transcription regulatory region sequence-specific DNA binding)|go:"GO:0004601"(peroxidase activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005777"(peroxisome)|go:"GO:0005782"(peroxisomal matrix)|go:"GO:0005829"(cytosol)|go:"GO:0006979"(response to oxidative stress)|go:"GO:0008379"(thioredoxin peroxidase activity)|go:"GO:0016209"(antioxidant activity)|go:"GO:0016480"(negative regulation of transcription by RNA polymerase III)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0032967"(positive regulation of collagen biosynthetic process)|go:"GO:0034599"(cellular response to oxidative stress)|go:"GO:0034614"(cellular response to reactive oxygen species)|go:"GO:0042744"(hydrogen peroxide catabolic process)|go:"GO:0043027"(cysteine-type endopeptidase inhibitor activity involved in apoptotic process)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0045454"(cell redox homeostasis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0051354"(negative regulation of oxidoreductase activity)|go:"GO:0051920"(peroxiredoxin activity)|go:"GO:0060785"(regulation of apoptosis involved in tissue homeostasis)|go:"GO:0070995"(NADPH oxidation)|go:"GO:0072541"(peroxynitrite reductase activity)|go:"GO:2001057"(reactive nitrogen species metabolic process)|interpro:IPR013740(Redoxin)|interpro:IPR036249|interpro:IPR037944|mint:P99029|reactome:R-MMU-3299685|reactome:R-MMU-5628897|interpro:IPR013766(Thioredoxin domain)	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:uWS2EcAdupLmsVsQ6h1AV+vRffs10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:O08709	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-444895|uniprotkb:Q91WT2|uniprotkb:Q9QWP4|uniprotkb:Q9QWW0	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:prdx6_mouse(display_long)|uniprotkb:Lysophosphatidylcholine acyltransferase 5(gene name synonym)|uniprotkb:Glutathione-dependent peroxiredoxin(gene name synonym)|uniprotkb:Antioxidant protein 2(gene name synonym)|uniprotkb:1-Cys peroxiredoxin(gene name synonym)|uniprotkb:Acidic calcium-independent phospholipase A2(gene name synonym)|uniprotkb:Non-selenium glutathione peroxidase(gene name synonym)|uniprotkb:Prdx6(gene name)|psi-mi:Prdx6(display_short)|uniprotkb:Aop2(gene name synonym)|uniprotkb:Ltw4(gene name synonym)|uniprotkb:Prdx5(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_031479.1|interpro:IPR000866(Alkyl hydroperoxide reductase/ Thiol specific antioxidant/ Mal allergen)|interpro:IPR024706|interpro:IPR019479|go:"GO:0000302"(response to reactive oxygen species)|go:"GO:0004601"(peroxidase activity)|go:"GO:0004602"(glutathione peroxidase activity)|go:"GO:0004623"(phospholipase A2 activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005764"(lysosome)|go:"GO:0005829"(cytosol)|go:"GO:0006979"(response to oxidative stress)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0032060"(bleb assembly)|go:"GO:0042744"(hydrogen peroxide catabolic process)|go:"GO:0042802"(identical protein binding)|go:"GO:0045454"(cell redox homeostasis)|go:"GO:0046475"(glycerophospholipid catabolic process)|go:"GO:0047184"(1-acylglycerophosphocholine O-acyltransferase activity)|go:"GO:0047499"(calcium-independent phospholipase A2 activity)|go:"GO:0048026"(positive regulation of mRNA splicing, via spliceosome)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0051920"(peroxiredoxin activity)|go:"GO:0098869"(cellular oxidant detoxification)|go:"GO:0102567"|go:"GO:0102568"|reactome:R-MMU-3299685|reactome:R-MMU-6798695|interpro:IPR013766(Thioredoxin domain)|mint:O08709|interpro:IPR036249	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:3Hnse2vkowtSGEobI2LJX6WrNl410090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P17742	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-1206043|uniprotkb:Q9CWJ5|uniprotkb:Q9R137|ensembl:ENSMUSP00000117987	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:ppia_mouse(display_long)|uniprotkb:Ppia(gene name)|psi-mi:Ppia(display_short)|uniprotkb:Cyclophilin A(gene name synonym)|uniprotkb:Cyclosporin A-binding protein(gene name synonym)|uniprotkb:SP18(gene name synonym)|uniprotkb:Rotamase A(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_032933.1|reactome:R-MMU-210991|reactome:R-MMU-6798695|rcsb pdb:6FK1|ensembl:ENSMUSG00000071866(gene)|ensembl:ENSMUST00000132846(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0000413"(protein peptidyl-prolyl isomerization)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0001934"(positive regulation of protein phosphorylation)|go:"GO:0003755"(peptidyl-prolyl cis-trans isomerase activity)|go:"GO:0005178"(integrin binding)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006457"(protein folding)|go:"GO:0006469"(negative regulation of protein kinase activity)|go:"GO:0006915"(apoptotic process)|go:"GO:0016018"(cyclosporin A binding)|go:"GO:0030168"(platelet activation)|go:"GO:0030182"(neuron differentiation)|go:"GO:0030593"(neutrophil chemotaxis)|go:"GO:0030595"(leukocyte chemotaxis)|go:"GO:0032148"(activation of protein kinase B activity)|go:"GO:0032873"(negative regulation of stress-activated MAPK cascade)|go:"GO:0032991"(protein-containing complex)|go:"GO:0034389"(lipid droplet organization)|go:"GO:0034599"(cellular response to oxidative stress)|go:"GO:0035307"(positive regulation of protein dephosphorylation)|go:"GO:0042118"(endothelial cell activation)|go:"GO:0043209"(myelin sheath)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0045069"(regulation of viral genome replication)|go:"GO:0045070"(positive regulation of viral genome replication)|go:"GO:0050714"(positive regulation of protein secretion)|go:"GO:0051092"(positive regulation of NF-kappaB transcription factor activity)|go:"GO:0060352"(cell adhesion molecule production)|go:"GO:0061944"(negative regulation of protein K48-linked ubiquitination)|go:"GO:0070527"(platelet aggregation)|go:"GO:1902176"(negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway)|go:"GO:1903901"(negative regulation of viral life cycle)|go:"GO:1904399"(heparan sulfate binding)|go:"GO:2001233"(regulation of apoptotic signaling pathway)|interpro:IPR002130(Peptidyl-prolyl cis-trans isomerase, cyclophilin-type)|interpro:IPR020892|interpro:IPR024936|interpro:IPR029000|mint:P17742	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:VwIoW2yfdaAEwZj6MfekFYqkZjw10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:O35621	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-20975328|ensembl:ENSMUSP00000023112	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:pmm1_mouse(display_long)|uniprotkb:Pmm1(gene name)|psi-mi:Pmm1(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000022474(gene)|ensembl:ENSMUST00000023112(transcript)|go:"GO:0004615"(phosphomannomutase activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006013"(mannose metabolic process)|go:"GO:0006487"(protein N-linked glycosylation)|go:"GO:0009298"(GDP-mannose biosynthetic process)|go:"GO:0043025"(neuronal cell body)|go:"GO:0046872"(metal ion binding)|go:"GO:1990830"(cellular response to leukemia inhibitory factor)|interpro:IPR005002(Eukaryotic phosphomannomutase)|interpro:IPR006379(HAD-superfamily hydrolase, subfamily IIB)|interpro:IPR023214|interpro:IPR036412|mint:O35621|reactome:R-MMU-446205|interpro:IPR043169|refseq:NP_038900.1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:a8YXtBGRJLhB/BVRD3SX+8QRUgw10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q3USB7	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-16733252|ensembl:ENSMUSP00000037854|uniprotkb:D3Z0W5|uniprotkb:Q3TSM9	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:plcl1_mouse(display_long)|uniprotkb:Phospholipase C-related but catalytically inactive protein(gene name synonym)|uniprotkb:Plcl1(gene name)|psi-mi:Plcl1(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000038349(gene)|ensembl:ENSMUST00000042986(transcript)|go:"GO:0004435"(phosphatidylinositol phospholipase C activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005886"(plasma membrane)|go:"GO:0006629"(lipid metabolic process)|go:"GO:0007214"(gamma-aminobutyric acid signaling pathway)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0033135"(regulation of peptidyl-serine phosphorylation)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0050811"(GABA receptor binding)|go:"GO:0070679"(inositol 1,4,5 trisphosphate binding)|go:"GO:0120163"(negative regulation of cold-induced thermogenesis)|go:"GO:1900122"(positive regulation of receptor binding)|interpro:IPR000008(C2 calcium-dependent membrane targeting)|interpro:IPR000909(Phospholipase C, phosphatidylinositol-specific , X region)|interpro:IPR001192("Phospholipase C, phosphoinositol-specific, C-terminal (PLC)")|interpro:IPR001711(Phospholipase C, phosphatidylinositol-specific, Y domain)|interpro:IPR001849(Pleckstrin-like)|interpro:IPR011992(EF-Hand type)|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR015359(Phospholipase C, phosphoinositol-specific, EF-hand-like)|interpro:IPR017946|interpro:IPR035892|interpro:IPR042124|mint:Q3USB7|refseq:NP_001108135.1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:UH43D5ZRmFajncAe1RTEkeZ68Pk10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q6ZPQ6	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-16744915|ensembl:ENSMUSP00000124111|uniprotkb:Q9R1P5	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:pitm2_mouse(display_long)|uniprotkb:Pitpnm2(gene name)|psi-mi:Pitpnm2(display_short)|uniprotkb:Drosophila retinal degeneration B homolog 2(gene name synonym)|uniprotkb:Kiaa1457(gene name synonym)|uniprotkb:Nir3(gene name synonym)|uniprotkb:Phosphatidylinositol transfer protein, membrane-associated 2(gene name synonym)|uniprotkb:Pyk2 N-terminal domain-interacting receptor 3(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000029406(gene)|ensembl:ENSMUST00000161938(transcript)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005543"(phospholipid binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005856"(cytoskeleton)|go:"GO:0008525"(phosphatidylcholine transporter activity)|go:"GO:0008526"(phosphatidylinositol transfer activity)|go:"GO:0012505"(endomembrane system)|go:"GO:0016020"(membrane)|go:"GO:0030971"(receptor tyrosine kinase binding)|go:"GO:0031210"(phosphatidylcholine binding)|go:"GO:0035091"(phosphatidylinositol binding)|go:"GO:0044297"(cell body)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|interpro:IPR001666(Phosphatidylinositol transfer protein)|interpro:IPR023214|interpro:IPR023393|interpro:IPR031315|interpro:IPR036412|mint:Q6ZPQ6|reactome:R-MMU-1483226|interpro:IPR004177(DDHD)|refseq:NP_001276401.1|refseq:NP_035386.1|refseq:XP_017176233.1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:4zrvxwjon6qsbpA3n5INhmMp/KQ10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:E9Q3L2	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-773641|uniprotkb:Q3UVT5|uniprotkb:Q6DIC7|uniprotkb:Q7TNG2|uniprotkb:Q80Y45	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:pi4ka_mouse(display_long)|uniprotkb:Pik4(gene name synonym)|uniprotkb:Pik4ca(gene name synonym)|uniprotkb:Pi4ka(gene name)|psi-mi:Pi4ka(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001001983.2|interpro:IPR016024(Armadillo-type fold)|interpro:IPR011009(Protein kinase-like)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR018936|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR042236|refseq:XP_006522097.1|interpro:IPR036940|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0016301"(kinase activity)|go:"GO:0016310"(phosphorylation)|go:"GO:0030660"(Golgi-associated vesicle membrane)|go:"GO:0039694"(viral RNA genome replication)|go:"GO:0044803"(multi-organism membrane organization)|go:"GO:0046786"(viral replication complex formation and maintenance)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|reactome:R-MMU-1483248|reactome:R-MMU-1660514|mint:E9Q3L2	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:IndUgD7njY39mo58MVLNKGSeD4c10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P12382	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-400474|uniprotkb:Q8VDX7|ensembl:ENSMUSP00000020522	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:pfkal_mouse(display_long)|uniprotkb:Pfkl(gene name)|psi-mi:Pfkl(display_short)|uniprotkb:Pfk-l(gene name synonym)|uniprotkb:Pfkb(gene name synonym)|uniprotkb:Phosphohexokinase(gene name synonym)|uniprotkb:Phosphofructo-1-kinase isozyme B(gene name synonym)|uniprotkb:6-phosphofructokinase type B(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_032852.2|ensembl:ENSMUSG00000020277(gene)|ensembl:ENSMUST00000020522(transcript)|go:"GO:0003872"(6-phosphofructokinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005829"(cytosol)|go:"GO:0005945"(6-phosphofructokinase complex)|go:"GO:0005975"(carbohydrate metabolic process)|go:"GO:0006002"(fructose 6-phosphate metabolic process)|go:"GO:0006096"(glycolytic process)|go:"GO:0009749"(response to glucose)|go:"GO:0016020"(membrane)|go:"GO:0016208"(AMP binding)|go:"GO:0019900"(kinase binding)|go:"GO:0030388"(fructose 1,6-bisphosphate metabolic process)|go:"GO:0042802"(identical protein binding)|go:"GO:0046676"(negative regulation of insulin secretion)|go:"GO:0046872"(metal ion binding)|go:"GO:0048029"(monosaccharide binding)|go:"GO:0061615"(glycolytic process through fructose-6-phosphate)|go:"GO:0061621"(canonical glycolysis)|go:"GO:0070061"(fructose binding)|go:"GO:0070095"(fructose-6-phosphate binding)|interpro:IPR000023(Phosphofructokinase)|interpro:IPR009161(6-phosphofructokinase, eukaryotic type)|interpro:IPR015912(Phosphofructokinase, conserved site)|interpro:IPR022953|interpro:IPR035966|interpro:IPR041914|mint:P12382|reactome:R-MMU-6798695|reactome:R-MMU-70171	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:WlBnp+GuMEVU1PRrdVHwJxZi5gE10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q8BKZ9	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-6877565|ensembl:ENSMUSP00000011058	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:odpx_mouse(display_long)|uniprotkb:Pdhx(gene name)|psi-mi:Pdhx(display_short)|uniprotkb:Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex(gene name synonym)|uniprotkb:Lipoyl-containing pyruvate dehydrogenase complex component X(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000010914(gene)|ensembl:ENSMUST00000011058(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005759"(mitochondrial matrix)|go:"GO:0016746"(acyltransferase activity)|go:"GO:0034604"("pyruvate dehydrogenase (NAD+) activity")|go:"GO:0045254"(pyruvate dehydrogenase complex)|go:"GO:0061732"(mitochondrial acetyl-CoA biosynthetic process from pyruvate)|interpro:IPR000089(Biotin/lipoyl attachment)|interpro:IPR001078(Catalytic domain of components of various dehydrogenase complexes)|interpro:IPR003016(2-oxo acid dehydrogenase, lipoyl-binding site)|interpro:IPR004167(E3 binding)|interpro:IPR011053(Single hybrid motif)|interpro:IPR023213|interpro:IPR036625|mint:Q8BKZ9|reactome:R-MMU-204174|reactome:R-MMU-389661|reactome:R-MMU-5362517|reactome:R-MMU-70268|refseq:NP_780303.1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:5b1n89M4YLpENMk4kqCsO2oQqJ010090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q61644	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-2255561|ensembl:ENSMUSP00000044168|ensembl:ENSMUSP00000094973|ensembl:ENSMUSP00000110523|ensembl:ENSMUSP00000155999|ensembl:ENSMUSP00000156003	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:pacn1_mouse(display_long)|uniprotkb:Pacsin(gene name synonym)|uniprotkb:Pacsin1(gene name)|psi-mi:Pacsin1(display_short)|uniprotkb:Syndapin-1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:XP_011244733.1|refseq:NP_035991.1|refseq:NP_848142.1|refseq:NP_001273672.1|refseq:NP_001273673.1|refseq:XP_006524289.1|dip:DIP-53108N|ensembl:ENSMUSG00000040276(gene)|ensembl:ENSMUST00000045896(transcript)|ensembl:ENSMUST00000097360(transcript)|ensembl:ENSMUST00000114873(transcript)|ensembl:ENSMUST00000231669(transcript)|ensembl:ENSMUST00000232437(transcript)|go:"GO:0005543"(phospholipid binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005768"(endosome)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0007010"(cytoskeleton organization)|go:"GO:0007015"(actin filament organization)|go:"GO:0007165"(signal transduction)|go:"GO:0008092"(cytoskeletal protein binding)|go:"GO:0030100"(regulation of endocytosis)|go:"GO:0030137"(COPI-coated vesicle)|go:"GO:0030659"(cytoplasmic vesicle membrane)|go:"GO:0032587"(ruffle membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043209"(myelin sheath)|go:"GO:0043679"(axon terminus)|go:"GO:0045202"(synapse)|go:"GO:0045806"(negative regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0048488"(synaptic vesicle endocytosis)|go:"GO:0048812"(neuron projection morphogenesis)|go:"GO:0072657"(protein localization to membrane)|go:"GO:0072659"(protein localization to plasma membrane)|go:"GO:0097320"(plasma membrane tubulation)|go:"GO:0098684"(photoreceptor ribbon synapse)|go:"GO:0098833"(presynaptic endocytic zone)|go:"GO:0098839"(postsynaptic density membrane)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099149"(regulation of postsynaptic neurotransmitter receptor internalization)|go:"GO:1900006"(positive regulation of dendrite development)|interpro:IPR001060(Cdc15/Fes/CIP4)|interpro:IPR001452(Src homology-3)|interpro:IPR027267|interpro:IPR028518|interpro:IPR031160|interpro:IPR035743|interpro:IPR036028|interpro:IPR037454|mint:Q61644|rcsb pdb:2X3V|rcsb pdb:2X3W|rcsb pdb:2X3X|reactome:R-MMU-8856828	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:lHj61yfdrObrknZUNqDUPgGI4cE10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q63912	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-774138	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:omgp_mouse(display_long)|uniprotkb:Omg(gene name)|psi-mi:Omg(display_short)|uniprotkb:Omgp(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	interpro:IPR032675|refseq:NP_062282.2|interpro:IPR001611(Leucine-rich repeat)|interpro:IPR003591(Leucine-rich repeat, typical subtype)|interpro:IPR000372(Leucine-rich repeat, cysteine-rich flanking region, N-terminal)|mint:Q63912|go:"GO:0005886"(plasma membrane)|go:"GO:0007155"(cell adhesion)|go:"GO:0022010"(central nervous system myelination)|go:"GO:0031102"(neuron projection regeneration)|go:"GO:0031225"(anchored component of membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0043209"(myelin sheath)|go:"GO:0048683"(regulation of collateral sprouting of intact axon in response to injury)|reactome:R-MMU-193634	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:iHphzgAn293E5bm07XyVXDtmG5w10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9CXZ1	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-2934359|uniprotkb:Q9WUB1	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:ndus4_mouse(display_long)|uniprotkb:Ndufs4(gene name)|psi-mi:Ndufs4(display_short)|uniprotkb:Complex I-18 kDa(gene name synonym)|uniprotkb:Complex I-AQDQ(gene name synonym)|uniprotkb:NADH-ubiquinone oxidoreductase 18 kDa subunit(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	interpro:IPR038532|reactome:R-MMU-6799198|go:"GO:0001932"(regulation of protein phosphorylation)|go:"GO:0003016"(respiratory system process)|go:"GO:0005739"(mitochondrion)|go:"GO:0005747"(mitochondrial respiratory chain complex I)|go:"GO:0006099"(tricarboxylic acid cycle)|go:"GO:0006954"(inflammatory response)|go:"GO:0007420"(brain development)|go:"GO:0007601"(visual perception)|go:"GO:0007605"(sensory perception of sound)|go:"GO:0007628"(adult walking behavior)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0022904"(respiratory electron transport chain)|go:"GO:0030534"(adult behavior)|go:"GO:0032981"(mitochondrial respiratory chain complex I assembly)|go:"GO:0036343"(psychomotor behavior)|go:"GO:0042417"(dopamine metabolic process)|go:"GO:0045087"(innate immune response)|go:"GO:0045333"(cellular respiration)|go:"GO:0048146"(positive regulation of fibroblast proliferation)|go:"GO:0050890"(cognition)|go:"GO:0051591"(response to cAMP)|go:"GO:0051881"(regulation of mitochondrial membrane potential)|go:"GO:0070997"(neuron death)|go:"GO:0071453"(cellular response to oxygen levels)|go:"GO:0072593"(reactive oxygen species metabolic process)|rcsb pdb:6G2J|rcsb pdb:6G72|rcsb pdb:6ZR2|rcsb pdb:6ZTQ|rcsb pdb:7AK5|rcsb pdb:7AK6|rcsb pdb:7B93|reactome:R-MMU-611105|go:"GO:0014876"(response to injury involved in regulation of muscle adaptation)|refseq:NP_035017.2|interpro:IPR006885(ETC complex I subunit conserved region)|mint:Q9CXZ1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:SbIn9Wz6DH4mipMhdL9qf55OJBo10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9CQC7	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-2255646|ensembl:ENSMUSP00000023514	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:ndub4_mouse(display_long)|uniprotkb:NADH-ubiquinone oxidoreductase B15 subunit(gene name synonym)|uniprotkb:Complex I-B15(gene name synonym)|uniprotkb:Ndufb4(gene name)|psi-mi:Ndufb4(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_080886.1|refseq:XP_001476621.1|refseq:XP_001478493.1|ensembl:ENSMUSG00000022820(gene)|ensembl:ENSMUST00000023514(transcript)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005747"(mitochondrial respiratory chain complex I)|go:"GO:0006979"(response to oxidative stress)|go:"GO:0032981"(mitochondrial respiratory chain complex I assembly)|interpro:IPR009866(NADH-ubiquinone oxidoreductase, subunit NDUFB4)|mint:Q9CQC7|rcsb pdb:6G2J|rcsb pdb:6G72|rcsb pdb:6ZR2|rcsb pdb:6ZTQ|rcsb pdb:7AK5|rcsb pdb:7AK6|rcsb pdb:7B93|reactome:R-MMU-611105|reactome:R-MMU-6799198	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:bOgcV3wmpY11CMgEaMUGzcugRiM10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9DC69	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-772731|intact:EBI-2934552|uniprotkb:Q6GTD3|uniprotkb:Q99JP9|ensembl:ENSMUSP00000144904	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:ndua9_mouse(display_long)|uniprotkb:NADH-ubiquinone oxidoreductase 39 kDa subunit(gene name synonym)|uniprotkb:Complex I-39kD(gene name synonym)|uniprotkb:Ndufa9(gene name)|psi-mi:Ndufa9(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_079634.2|ensembl:ENSMUSG00000000399(gene)|ensembl:ENSMUST00000205002(transcript)|go:"GO:0003954"(NADH dehydrogenase activity)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005747"(mitochondrial respiratory chain complex I)|go:"GO:0005759"(mitochondrial matrix)|go:"GO:0007623"(circadian rhythm)|go:"GO:0009749"(response to glucose)|go:"GO:0031966"(mitochondrial membrane)|go:"GO:0032981"(mitochondrial respiratory chain complex I assembly)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:1901006"(ubiquinone-6 biosynthetic process)|interpro:IPR008030(NmrA-like)|interpro:IPR036291|mint:Q9DC69|rcsb pdb:6G2J|rcsb pdb:6G72|rcsb pdb:6ZR2|rcsb pdb:6ZTQ|rcsb pdb:7AK5|rcsb pdb:7AK6|rcsb pdb:7B93|reactome:R-MMU-611105|reactome:R-MMU-6799198	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:cyWl9Af7lg/hJx/mANKhMVV4jbQ10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q3V3R1	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-912208|uniprotkb:Q3TVQ0|uniprotkb:Q3V402|uniprotkb:Q8CAL0|uniprotkb:Q8K2N5|uniprotkb:Q80V98|ensembl:ENSMUSP00000036178|ensembl:ENSMUSP00000112870|ensembl:ENSMUSP00000112897	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:c1tm_mouse(display_long)|uniprotkb:Formyltetrahydrofolate synthetase(gene name synonym)|uniprotkb:Mthfd1l(gene name)|psi-mi:Mthfd1l(display_short)|uniprotkb:Fthfsdc1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001164256.1|refseq:NP_001164257.1|refseq:NP_758512.3|ensembl:ENSMUSG00000040675(gene)|ensembl:ENSMUST00000043735(transcript)|ensembl:ENSMUST00000117291(transcript)|ensembl:ENSMUST00000120585(transcript)|go:"GO:0001843"(neural tube closure)|go:"GO:0004329"(formate-tetrahydrofolate ligase activity)|go:"GO:0004488"("methylenetetrahydrofolate dehydrogenase (NADP+) activity")|go:"GO:0005524"(ATP binding)|go:"GO:0005739"(mitochondrion)|go:"GO:0005829"(cytosol)|go:"GO:0006760"(folic acid-containing compound metabolic process)|go:"GO:0009257"(10-formyltetrahydrofolate biosynthetic process)|go:"GO:0015942"(formate metabolic process)|go:"GO:0035999"(tetrahydrofolate interconversion)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0048702"(embryonic neurocranium morphogenesis)|go:"GO:0048703"(embryonic viscerocranium morphogenesis)|interpro:IPR000559(Formate-tetrahydrofolate ligase, FTHFS)|interpro:IPR000672(Tetrahydrofolate dehydrogenase/cyclohydrolase)|interpro:IPR020628|interpro:IPR020630|interpro:IPR020631|interpro:IPR027417|interpro:IPR036291|mint:Q3V3R1|rcsb pdb:2EO2|reactome:R-MMU-196757	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:Hh58kyg8+iN4T2flIZSrUkUf1MI10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q791V5	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-775885|ensembl:ENSMUSP00000121851|uniprotkb:Q3TPS5|uniprotkb:Q99LZ6|uniprotkb:Q9D060|uniprotkb:Q9D7Y2|uniprotkb:Q9QZP3	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:mtch2_mouse(display_long)|uniprotkb:Mtch2(gene name)|psi-mi:Mtch2(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043280"(positive regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0045820"(negative regulation of glycolytic process)|go:"GO:0061484"(hematopoietic stem cell homeostasis)|go:"GO:0070585"(protein localization to mitochondrion)|go:"GO:0071478"(cellular response to radiation)|go:"GO:0090152"(establishment of protein localization to mitochondrial membrane involved in mitochondrial fission)|go:"GO:0097284"(hepatocyte apoptotic process)|go:"GO:1902108"(regulation of mitochondrial membrane permeability involved in apoptotic process)|ensembl:ENSMUSG00000027282(gene)|ensembl:ENSMUST00000136872(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0006089"(lactate metabolic process)|go:"GO:0010917"(negative regulation of mitochondrial membrane potential)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0035701"(hematopoietic stem cell migration)|go:"GO:0042775"(mitochondrial ATP synthesis coupled electron transport)|go:"GO:1902231"(positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage)|interpro:IPR018108|interpro:IPR023395|mint:Q791V5|refseq:NP_062732.1|refseq:NP_001304170.1|refseq:NP_001304171.1|refseq:NP_001304172.1|refseq:NP_001304173.1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:5TT13uYcx21wiKjuQc4tynZ31Ac10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9JLB0	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-771456|uniprotkb:Q9JLB1|uniprotkb:Q9WV37|ensembl:ENSMUSP00000038772|ensembl:ENSMUSP00000125880	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:pals2_mouse(display_long)|uniprotkb:Pals2(gene name)|psi-mi:Pals2(display_short)|uniprotkb:Dlgh4(gene name synonym)|uniprotkb:Dlgh4 protein(gene name synonym)|uniprotkb:MAGUK p55 subfamily member 6(gene name synonym)|uniprotkb:Mpp6(gene name synonym)|uniprotkb:P55T protein(gene name synonym)|uniprotkb:Protein associated with Lin-7 2(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001158205.1|refseq:NP_001158206.1|refseq:NP_064323.2|interpro:IPR001478(PDZ/DHR/GLGF)|interpro:IPR004172(L27)|interpro:IPR008144(Guanylate kinase)|interpro:IPR008145(Guanylate kinase/L-type calcium channel region)|interpro:IPR014775(L27, C-terminal)|interpro:IPR020590|interpro:IPR027417|interpro:IPR035603|interpro:IPR036028|interpro:IPR036034|interpro:IPR036892|mint:Q9JLB0|ensembl:ENSMUSG00000038388(gene)|ensembl:ENSMUST00000036225(transcript)|ensembl:ENSMUST00000166318(transcript)|go:"GO:0004385"(guanylate kinase activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|interpro:IPR001452(Src homology-3)	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:gMNWZ/pRWUn+VfBE5m6SZc9IvqA10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9D023	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-2693979|uniprotkb:Q3UA44|ensembl:ENSMUSP00000027853	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:mpc2_mouse(display_long)|uniprotkb:Mpc2(gene name)|psi-mi:Mpc2(display_short)|uniprotkb:Brain protein 44(gene name synonym)|uniprotkb:Brp44(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_081706.1|ensembl:ENSMUSG00000026568(gene)|ensembl:ENSMUST00000027853(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0006850"(mitochondrial pyruvate transmembrane transport)|go:"GO:0031305"(integral component of mitochondrial inner membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0061732"(mitochondrial acetyl-CoA biosynthetic process from pyruvate)|interpro:IPR005336(Protein of unknown function UPF0041)|mint:Q9D023|go:"GO:0035774"(positive regulation of insulin secretion involved in cellular response to glucose stimulus)	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:PDFMxMPgkUM9G0QKud00Lk/kors10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q8VHK5	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-26595938|ensembl:ENSMUSP00000047667	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:mlc1_mouse(display_long)|uniprotkb:Mlc1(gene name)|psi-mi:Mlc1(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	go:"GO:0097450"(astrocyte end-foot)|interpro:IPR033280|mint:Q8VHK5|ensembl:ENSMUSG00000035805(gene)|ensembl:ENSMUST00000042594(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0005764"(lysosome)|go:"GO:0005768"(endosome)|go:"GO:0005769"(early endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005901"(caveola)|go:"GO:0005911"(cell-cell junction)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0016323"(basolateral plasma membrane)|go:"GO:0016324"(apical plasma membrane)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0032388"(positive regulation of intracellular transport)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0042802"(identical protein binding)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045121"(membrane raft)|go:"GO:0047484"(regulation of response to osmotic stress)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0055037"(recycling endosome)|go:"GO:0071397"(cellular response to cholesterol)|go:"GO:0072584"(caveolin-mediated endocytosis)|refseq:NP_573504.1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:0jUNG8/4rNL4CJ0ZC7jXFfrkKeQ10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q99MR8	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-773469|uniprotkb:Q3TGU0|uniprotkb:Q9D8R2|ensembl:ENSMUSP00000029259	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:mcca_mouse(display_long)|uniprotkb:3-methylcrotonyl-CoA carboxylase 1(gene name synonym)|uniprotkb:"3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha"(gene name synonym)|uniprotkb:3-methylcrotonyl-CoA carboxylase biotin-containing subunit(gene name synonym)|uniprotkb:Mccc1(gene name)|psi-mi:Mccc1(display_short)|uniprotkb:Mcca(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_076133.3|ensembl:ENSMUSG00000027709(gene)|ensembl:ENSMUST00000029259(transcript)|go:"GO:0002169"(3-methylcrotonyl-CoA carboxylase complex, mitochondrial)|go:"GO:0004485"(methylcrotonoyl-CoA carboxylase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005759"(mitochondrial matrix)|go:"GO:0006552"(leucine catabolic process)|go:"GO:0016421"(CoA carboxylase activity)|go:"GO:0046872"(metal ion binding)|go:"GO:1905202"(methylcrotonoyl-CoA carboxylase complex)|interpro:IPR000089(Biotin/lipoyl attachment)|interpro:IPR001882(Biotin-binding site)|interpro:IPR005479(Carbamoyl phosphate synthetase, large subunit, ATP-binding)|interpro:IPR005481(Carbamoyl phosphate synthase, large subunit, N-terminal)|interpro:IPR005482(Biotin carboxylase, C-terminal)|interpro:IPR011053(Single hybrid motif)|interpro:IPR011054(Rudiment single hybrid motif)|interpro:IPR011761(ATP-grasp fold)|interpro:IPR011764(Biotin carboxylation region)|interpro:IPR016185(PreATP-grasp-like fold)|mint:Q99MR8|reactome:R-MMU-196780|reactome:R-MMU-70895	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:SziuYdcOx+maKy71pNxoKql1HGI10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q8R001	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-774541|uniprotkb:Q3UF61|uniprotkb:Q8BLZ6|uniprotkb:Q8BYR6|uniprotkb:Q8C177|uniprotkb:Q8K109|ensembl:ENSMUSP00000025127	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:mare2_mouse(display_long)|uniprotkb:End-binding protein 2(gene name synonym)|uniprotkb:APC-binding protein EB2(gene name synonym)|uniprotkb:Mapre2(gene name)|psi-mi:Mapre2(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001156413.1|refseq:NP_001156414.1|refseq:NP_694698.3|ensembl:ENSMUSG00000024277(gene)|ensembl:ENSMUST00000025127(transcript)|go:"GO:0005815"(microtubule organizing center)|go:"GO:0005881"(cytoplasmic microtubule)|go:"GO:0005925"(focal adhesion)|go:"GO:0019901"(protein kinase binding)|go:"GO:0031110"(regulation of microtubule polymerization or depolymerization)|go:"GO:0032014"(positive regulation of ARF protein signal transduction)|go:"GO:0035371"(microtubule plus-end)|go:"GO:0042802"(identical protein binding)|go:"GO:0043547"(positive regulation of GTPase activity)|go:"GO:0051010"(microtubule plus-end binding)|go:"GO:0051225"(spindle assembly)|go:"GO:0051233"(spindle midzone)|go:"GO:0051301"(cell division)|go:"GO:0051549"(positive regulation of keratinocyte migration)|go:"GO:0120183"(positive regulation of focal adhesion disassembly)|go:"GO:1904825"(protein localization to microtubule plus-end)|interpro:IPR001715(Calponin-like actin-binding)|interpro:IPR004953(EB1, C-terminal)|interpro:IPR027328|interpro:IPR027735|interpro:IPR036133|interpro:IPR036872|mint:Q8R001	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:NsL/q4JJQQNsirZNw3YMqr5GcvA10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P27546	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-2526714|uniprotkb:Q05BJ2|uniprotkb:Q3UIS2|uniprotkb:Q3UUH5|uniprotkb:Q3UY36|uniprotkb:Q7TPC6|uniprotkb:Q7TPD4|uniprotkb:Q80YQ5|uniprotkb:Q8CFP5|ensembl:ENSMUSP00000035055	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:map4_mouse(display_long)|uniprotkb:Map4(gene name)|psi-mi:Map4(display_short)|uniprotkb:Mtap4(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001192259.1|refseq:NP_001192261.1|refseq:NP_032659.2|ensembl:ENSMUSG00000032479(gene)|ensembl:ENSMUST00000035055(transcript)|go:"GO:0000226"(microtubule cytoskeleton organization)|go:"GO:0005829"(cytosol)|go:"GO:0005874"(microtubule)|go:"GO:0005886"(plasma membrane)|go:"GO:0005930"(axoneme)|go:"GO:0007052"(mitotic spindle organization)|go:"GO:0008017"(microtubule binding)|go:"GO:0014069"(postsynaptic density)|go:"GO:0015630"(microtubule cytoskeleton)|go:"GO:0030424"(axon)|go:"GO:0031175"(neuron projection development)|go:"GO:0051012"(microtubule sliding)|go:"GO:0051294"(establishment of spindle orientation)|go:"GO:0051301"(cell division)|go:"GO:0072686"(mitotic spindle)|go:"GO:1902856"(negative regulation of non-motile cilium assembly)|interpro:IPR001084(Tubulin-binding Tau protein)|interpro:IPR027323|mint:P27546|go:"GO:0043005"(neuron projection)|refseq:NP_001298092.1|refseq:NP_001298093.1|refseq:XP_017168653.1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:DVblnhNfUaxX6rpvxn660Pq09L410090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P14873	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-764653|uniprotkb:E9QM11|ensembl:ENSMUSP00000068374	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:map1b_mouse(display_long)|uniprotkb:MAP1.2(gene name synonym)|uniprotkb:"MAP1(X)"(gene name synonym)|uniprotkb:Map1b(gene name)|psi-mi:Map1b(display_short)|uniprotkb:Mtap1b(gene name synonym)|uniprotkb:Mtap5(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_032660.2|ensembl:ENSMUSG00000052727(gene)|ensembl:ENSMUST00000064762(transcript)|go:"GO:0000226"(microtubule cytoskeleton organization)|go:"GO:0001578"(microtubule bundle formation)|go:"GO:0001750"(photoreceptor outer segment)|go:"GO:0003779"(actin binding)|go:"GO:0005519"(cytoskeletal regulatory protein binding)|go:"GO:0005543"(phospholipid binding)|go:"GO:0005829"(cytosol)|go:"GO:0005856"(cytoskeleton)|go:"GO:0005874"(microtubule)|go:"GO:0005875"(microtubule associated complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0007017"(microtubule-based process)|go:"GO:0007026"(negative regulation of microtubule depolymerization)|go:"GO:0007409"(axonogenesis)|go:"GO:0007416"(synapse assembly)|go:"GO:0008017"(microtubule binding)|go:"GO:0009612"(response to mechanical stimulus)|go:"GO:0009743"(response to carbohydrate)|go:"GO:0009987"(cellular process)|go:"GO:0010035"(response to inorganic substance)|go:"GO:0014012"(peripheral nervous system axon regeneration)|go:"GO:0014069"(postsynaptic density)|go:"GO:0017085"(response to insecticide)|go:"GO:0021700"(developmental maturation)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0030426"(growth cone)|go:"GO:0031114"(regulation of microtubule depolymerization)|go:"GO:0031116"(positive regulation of microtubule polymerization)|go:"GO:0032355"(response to estradiol)|go:"GO:0032387"(negative regulation of intracellular transport)|go:"GO:0033189"(response to vitamin A)|go:"GO:0036477"(somatodendritic compartment)|go:"GO:0042493"|go:"GO:0043025"(neuronal cell body)|go:"GO:0043196"(varicosity)|go:"GO:0043197"(dendritic spine)|go:"GO:0043204"(perikaryon)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045202"(synapse)|go:"GO:0016358"(dendrite development)|go:"GO:0045666"(positive regulation of neuron differentiation)|go:"GO:0045773"(positive regulation of axon extension)|go:"GO:0047497"(mitochondrion transport along microtubule)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0048675"(axon extension)|go:"GO:0051915"(induction of synaptic plasticity by chemical substance)|go:"GO:0061162"(establishment of monopolar cell polarity)|go:"GO:0071363"(cellular response to growth factor stimulus)|go:"GO:0071375"(cellular response to peptide hormone stimulus)|go:"GO:0097440"(apical dendrite)|go:"GO:0097441"(basal dendrite)|go:"GO:0097457"(hippocampal mossy fiber)|interpro:IPR000102(Neuraxin/MAP1B repeat)|interpro:IPR026074|interpro:IPR027321|mint:P14873	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:8Ph5u01KvXbfedKGBXu5cGckp7Y10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9QYR6	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-774021|uniprotkb:A2ARN9|uniprotkb:Q3TQM8|uniprotkb:Q3TUV8|uniprotkb:Q3UHB7|uniprotkb:Q9QZH9|uniprotkb:Q9QZI0|uniprotkb:Q9QZI1|ensembl:ENSMUSP00000106269	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:map1a_mouse(display_long)|uniprotkb:Map1a(gene name)|psi-mi:Map1a(display_short)|uniprotkb:Mtap1(gene name synonym)|uniprotkb:Mtap1a(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001166977.1|refseq:NP_115769.1|ensembl:ENSMUSG00000027254(gene)|ensembl:ENSMUST00000110639(transcript)|go:"GO:0000226"(microtubule cytoskeleton organization)|go:"GO:0001750"(photoreceptor outer segment)|go:"GO:0003779"(actin binding)|go:"GO:0005518"(collagen binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005874"(microtubule)|go:"GO:0005875"(microtubule associated complex)|go:"GO:0007026"(negative regulation of microtubule depolymerization)|go:"GO:0007409"(axonogenesis)|go:"GO:0007605"(sensory perception of sound)|go:"GO:0007613"(memory)|go:"GO:0008017"(microtubule binding)|go:"GO:0008093"(cytoskeletal anchor activity)|go:"GO:0008306"(associative learning)|go:"GO:0014069"(postsynaptic density)|go:"GO:0015631"(tubulin binding)|go:"GO:0016358"(dendrite development)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0031114"(regulation of microtubule depolymerization)|go:"GO:0032435"(negative regulation of proteasomal ubiquitin-dependent protein catabolic process)|go:"GO:0035255"(ionotropic glutamate receptor binding)|go:"GO:0043005"(neuron projection)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043194"(axon initial segment)|go:"GO:0043198"(dendritic shaft)|go:"GO:0044307"(dendritic branch)|go:"GO:0045202"(synapse)|go:"GO:0045494"(photoreceptor cell maintenance)|go:"GO:0048167"(regulation of synaptic plasticity)|go:"GO:0050882"(voluntary musculoskeletal movement)|go:"GO:0070050"(neuron cellular homeostasis)|go:"GO:0099641"(anterograde axonal protein transport)|go:"GO:0099642"(retrograde axonal protein transport)|go:"GO:0150001"(primary dendrite)|go:"GO:1901588"(dendritic microtubule)|go:"GO:1902817"(negative regulation of protein localization to microtubule)|go:"GO:1903829"(positive regulation of protein localization)|go:"GO:1904115"(axon cytoplasm)|go:"GO:1990535"(neuron projection maintenance)|go:"GO:2000010"(positive regulation of protein localization to cell surface)|interpro:IPR015656(MAP1A)|interpro:IPR026074|interpro:IPR036866|mint:Q9QYR6|rcsb pdb:5GNV	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:YFVfrv4h8ioQ9vL1A9LYsA5xriQ10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P28738	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-2506834|uniprotkb:Q6NXI9|ensembl:ENSMUSP00000028102|uniprotkb:Q9Z2F8	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:kif5c_mouse(display_long)|uniprotkb:Kif5c(gene name)|psi-mi:Kif5c(display_short)|uniprotkb:Nkhc2(gene name synonym)|uniprotkb:Kinesin heavy chain neuron-specific 2(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000026764(gene)|ensembl:ENSMUST00000028102(transcript)|go:"GO:0003777"(microtubule motor activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005871"(kinesin complex)|go:"GO:0005874"(microtubule)|go:"GO:0007018"(microtubule-based movement)|go:"GO:0007411"(axon guidance)|go:"GO:0008017"(microtubule binding)|go:"GO:0008045"(motor neuron axon guidance)|go:"GO:0008574"(plus-end-directed microtubule motor activity)|go:"GO:0030425"(dendrite)|go:"GO:0030705"(cytoskeleton-dependent intracellular transport)|go:"GO:0032839"(dendrite cytoplasm)|go:"GO:0034190"(apolipoprotein receptor binding)|go:"GO:0035253"(ciliary rootlet)|go:"GO:0043005"(neuron projection)|go:"GO:0043025"(neuronal cell body)|go:"GO:0044295"(axonal growth cone)|go:"GO:0046034"(ATP metabolic process)|go:"GO:0048489"(synaptic vesicle transport)|go:"GO:0051028"(mRNA transport)|go:"GO:0098963"(dendritic transport of messenger ribonucleoprotein complex)|go:"GO:0098964"(anterograde dendritic transport of messenger ribonucleoprotein complex)|go:"GO:0098971"(anterograde dendritic transport of neurotransmitter receptor complex)|go:"GO:0099641"(anterograde axonal protein transport)|go:"GO:0150034"(distal axon)|go:"GO:1904115"(axon cytoplasm)|interpro:IPR001752(Kinesin, motor region)|interpro:IPR019821|interpro:IPR027417|interpro:IPR036961|mint:P28738|rcsb pdb:1VFV|rcsb pdb:1VFW|rcsb pdb:1VFX|rcsb pdb:1VFZ|rcsb pdb:3J6H|rcsb pdb:3WRD|rcsb pdb:3X2T|rcsb pdb:5HLE|refseq:NP_032475.2	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:GbPyKDztqPG8BP2+44k3TXPuI/010090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P28740	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-776122|uniprotkb:O54744|uniprotkb:Q91W03|ensembl:ENSMUSP00000022204	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:kif2a_mouse(display_long)|uniprotkb:Kif2a(gene name)|psi-mi:Kif2a(display_short)|uniprotkb:Kif2(gene name synonym)|uniprotkb:Kns2(gene name synonym)|uniprotkb:Kinesin-2(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001139251.1|refseq:XP_006517602.1|ensembl:ENSMUSG00000021693(gene)|ensembl:ENSMUST00000022204(transcript)|go:"GO:0000226"(microtubule cytoskeleton organization)|go:"GO:0000922"(spindle pole)|go:"GO:0003777"(microtubule motor activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005730"(nucleolus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005764"(lysosome)|go:"GO:0005813"(centrosome)|go:"GO:0005814"(centriole)|go:"GO:0005819"(spindle)|go:"GO:0005871"(kinesin complex)|go:"GO:0005874"(microtubule)|go:"GO:0007018"(microtubule-based movement)|go:"GO:0007052"(mitotic spindle organization)|go:"GO:0007399"(nervous system development)|go:"GO:0008017"(microtubule binding)|go:"GO:0016604"(nuclear body)|go:"GO:0019901"(protein kinase binding)|go:"GO:0030154"(cell differentiation)|go:"GO:0030334"(regulation of cell migration)|go:"GO:0051301"(cell division)|go:"GO:0090307"(mitotic spindle assembly)|go:"GO:0097228"(sperm principal piece)|go:"GO:0120103"(centriolar subdistal appendage)|go:"GO:1990939"|interpro:IPR001752(Kinesin, motor region)|interpro:IPR019821|interpro:IPR027417|interpro:IPR027640|interpro:IPR036961|mint:P28740|reactome:R-MMU-141444|reactome:R-MMU-2132295|reactome:R-MMU-2467813|reactome:R-MMU-2500257|reactome:R-MMU-5663220|reactome:R-MMU-6811434|reactome:R-MMU-68877|reactome:R-MMU-9648025|reactome:R-MMU-983189	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:xPWg0rzeKQooA3AM2Ics+e/QnRM10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P62482	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-771096|uniprotkb:P97381|uniprotkb:Q60942|uniprotkb:Q64284|ensembl:ENSMUSP00000101273|ensembl:ENSMUSP00000125058	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:kcab2_mouse(display_long)|uniprotkb:"K(+) channel subunit beta-2"(gene name synonym)|uniprotkb:Kv-beta-2(gene name synonym)|uniprotkb:Neuroimmune protein F5(gene name synonym)|uniprotkb:Kcnab2(gene name)|psi-mi:Kcnab2(display_short)|uniprotkb:Ckbeta2(gene name synonym)|uniprotkb:I2rf5(gene name synonym)|uniprotkb:Kcnb3(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_034728.2|refseq:XP_017175485.1|refseq:NP_001239585.1|ensembl:ENSMUSG00000028931(gene)|ensembl:ENSMUST00000105648(transcript)|ensembl:ENSMUST00000160884(transcript)|go:"GO:0002244"(hematopoietic progenitor cell differentiation)|go:"GO:0004033"("aldo-keto reductase (NADP) activity")|go:"GO:0005249"(voltage-gated potassium channel activity)|go:"GO:0005829"(cytosol)|go:"GO:0005856"(cytoskeleton)|go:"GO:0008076"(voltage-gated potassium channel complex)|go:"GO:0014069"(postsynaptic density)|go:"GO:0015459"(potassium channel regulator activity)|go:"GO:0016020"(membrane)|go:"GO:0030424"(axon)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0034705"(potassium channel complex)|go:"GO:0043005"(neuron projection)|go:"GO:0043679"(axon terminus)|go:"GO:0044224"(juxtaparanode region of axon)|go:"GO:0044325"(transmembrane transporter binding)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0047834"(D-threo-aldose 1-dehydrogenase activity)|go:"GO:0050905"(neuromuscular process)|go:"GO:0070995"(NADPH oxidation)|go:"GO:0098900"(regulation of action potential)|go:"GO:1901379"(regulation of potassium ion transmembrane transport)|go:"GO:1990031"(pinceau fiber)|go:"GO:2000008"(regulation of protein localization to cell surface)|interpro:IPR005399(Potassium channel, voltage-dependent, beta subunit, KCNAB-related)|interpro:IPR005401(Potassium channel, voltage-dependent, beta subunit, KCNAB2)|interpro:IPR005983(Potassium channel, voltage-dependent, beta subunit, KCNAB)|interpro:IPR023210|interpro:IPR036812|mint:P62482|reactome:R-MMU-1296072|reactome:R-MMU-6798695	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:ZWu/Ez0ginUniSAUbIGXgod3iqg10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9Z0R4	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-645386|uniprotkb:Q9R143|uniprotkb:F8VQE5|ensembl:ENSMUSP00000109635	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:itsn1_mouse(display_long)|uniprotkb:EH and SH3 domains protein 1(gene name synonym)|uniprotkb:Itsn1(gene name)|psi-mi:Itsn1(display_short)|uniprotkb:Ese1(gene name synonym)|uniprotkb:Itsn(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001103745.1|refseq:NP_034717.2|ensembl:ENSMUSG00000022957(gene)|ensembl:ENSMUST00000114002(transcript)|go:"GO:0005085"(guanyl-nucleotide exchange factor activity)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005635"(nuclear envelope)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0006887"(exocytosis)|go:"GO:0006897"(endocytosis)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0015031"(protein transport)|go:"GO:0016197"(endosomal transport)|go:"GO:0019209"(kinase activator activity)|go:"GO:0030027"(lamellipodium)|go:"GO:0030139"(endocytic vesicle)|go:"GO:0034613"(cellular protein localization)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043197"(dendritic spine)|go:"GO:0043524"(negative regulation of neuron apoptotic process)|go:"GO:0044305"(calyx of Held)|go:"GO:0045202"(synapse)|go:"GO:0048488"(synaptic vesicle endocytosis)|go:"GO:0051897"(positive regulation of protein kinase B signaling)|go:"GO:0055037"(recycling endosome)|go:"GO:0060090"(molecular adaptor activity)|go:"GO:0060124"(positive regulation of growth hormone secretion)|go:"GO:0060999"(positive regulation of dendritic spine development)|go:"GO:0070064"(proline-rich region binding)|go:"GO:0097440"(apical dendrite)|go:"GO:0097708"(intracellular vesicle)|go:"GO:0098833"(presynaptic endocytic zone)|go:"GO:0098871"(postsynaptic actin cytoskeleton)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0150007"(clathrin-dependent synaptic vesicle endocytosis)|go:"GO:1905274"(regulation of modification of postsynaptic actin cytoskeleton)|go:"GO:2001288"(positive regulation of caveolin-mediated endocytosis)|interpro:IPR000008(C2 calcium-dependent membrane targeting)|interpro:IPR000219("Dbl homology (DH) domain")|interpro:IPR000261("EPS15 homology (EH)")|interpro:IPR001331(Guanine-nucleotide dissociation stimulator, CDC24, conserved site)|interpro:IPR001452(Src homology-3)|interpro:IPR001849(Pleckstrin-like)|interpro:IPR002048(Calcium-binding EF-hand)|interpro:IPR011992(EF-Hand type)|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR018247|interpro:IPR035892|interpro:IPR035899|interpro:IPR036028|mint:Q9Z0R4|rcsb pdb:3HS8|rcsb pdb:3JV3|reactome:R-MMU-193648|reactome:R-MMU-3928662|reactome:R-MMU-416482|reactome:R-MMU-8856825|reactome:R-MMU-8856828|reactome:R-MMU-9013148|reactome:R-MMU-9013406|reactome:R-MMU-9013408	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:naFu4D6P1S+/t5+VVVeb0De8lEg10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q8R071	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-400175|uniprotkb:A2AQ20|ensembl:ENSMUSP00000028758	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:ip3ka_mouse(display_long)|uniprotkb:Itpka(gene name)|psi-mi:Itpka(display_short)|uniprotkb:Inositol 1,4,5-trisphosphate 3-kinase A(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_666237.1|ensembl:ENSMUSG00000027296(gene)|ensembl:ENSMUST00000028758(transcript)|go:"GO:0000828"(inositol hexakisphosphate kinase activity)|go:"GO:0004683"(calmodulin-dependent protein kinase activity)|go:"GO:0005516"(calmodulin binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0006020"(inositol metabolic process)|go:"GO:0008440"(inositol-1,4,5-trisphosphate 3-kinase activity)|go:"GO:0016301"(kinase activity)|go:"GO:0030036"(actin cytoskeleton organization)|go:"GO:0031267"(small GTPase binding)|go:"GO:0032958"(inositol phosphate biosynthetic process)|go:"GO:0043197"(dendritic spine)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048167"(regulation of synaptic plasticity)|go:"GO:0061003"(positive regulation of dendritic spine morphogenesis)|go:"GO:0097062"(dendritic spine maintenance)|interpro:IPR005522(Inositol polyphosphate kinase)|interpro:IPR038286|mint:Q8R071|reactome:R-MMU-1855204	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:jbKxFPr5BAsFC6V2IFEW7U6PBcA10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q8BU30	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-6909077|ensembl:ENSMUSP00000048096|ensembl:ENSMUSP00000126806|ensembl:ENSMUSP00000132082|uniprotkb:Q6NXK4	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:syic_mouse(display_long)|uniprotkb:Iars(gene name synonym)|uniprotkb:Isoleucyl-tRNA synthetase(gene name synonym)|uniprotkb:Iars1(gene name)|psi-mi:Iars1(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:XP_006516889.1|ensembl:ENSMUSG00000037851(gene)|ensembl:ENSMUST00000047363(transcript)|ensembl:ENSMUST00000164260(transcript)|ensembl:ENSMUST00000165316(transcript)|go:"GO:0000049"(tRNA binding)|go:"GO:0002161"(aminoacyl-tRNA editing activity)|go:"GO:0004822"(isoleucine-tRNA ligase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006428"(isoleucyl-tRNA aminoacylation)|go:"GO:0017101"(aminoacyl-tRNA synthetase multienzyme complex)|go:"GO:0051020"(GTPase binding)|interpro:IPR001412(Aminoacyl-tRNA synthetase, class I, conserved site)|interpro:IPR002300(Aminoacyl-tRNA synthetase, class Ia)|interpro:IPR002301(Isoleucyl-tRNA synthetase, class Ia)|interpro:IPR009008(Valyl/Leucyl/Isoleucyl-tRNA synthetase, class Ia, editing)|interpro:IPR009080(Aminoacyl-tRNA synthetase, class 1a, anticodon-binding)|interpro:IPR013155(Valyl/Leucyl/Isoleucyl-tRNA synthetase, class I, anticodon-binding)|interpro:IPR014729(Rossmann-like alpha/beta/alpha sandwich fold)|interpro:IPR023586|interpro:IPR033709|mint:Q8BU30|refseq:NP_742012.2	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:xOIl+VyRSco1eDBN0Cs35nQSk3I10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:O70503	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-773393|uniprotkb:Q3TID1|uniprotkb:Q3U7V9|uniprotkb:Q542N3|uniprotkb:Q8CI39|ensembl:ENSMUSP00000028619	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:dhb12_mouse(display_long)|uniprotkb:17-beta-hydroxysteroid dehydrogenase 12(gene name synonym)|uniprotkb:3-ketoacyl-CoA reductase(gene name synonym)|uniprotkb:KIK-I(gene name synonym)|uniprotkb:Hsd17b12(gene name)|psi-mi:Hsd17b12(display_short)|uniprotkb:Kik1(gene name synonym)|uniprotkb:Estradiol 17-beta-dehydrogenase 12(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_062631.1|ensembl:ENSMUSG00000027195(gene)|ensembl:ENSMUST00000028619(transcript)|go:"GO:0001968"(fibronectin binding)|go:"GO:0005518"(collagen binding)|go:"GO:0006633"(fatty acid biosynthetic process)|go:"GO:0006703"(estrogen biosynthetic process)|go:"GO:0008201"(heparin binding)|go:"GO:0009923"(fatty acid elongase complex)|go:"GO:0010811"(positive regulation of cell-substrate adhesion)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030198"(extracellular matrix organization)|go:"GO:0031012"(extracellular matrix)|go:"GO:0044594"("17-beta-hydroxysteroid dehydrogenase (NAD+) activity")|go:"GO:0050062"(long-chain-fatty-acyl-CoA reductase activity)|go:"GO:0072582"("17-beta-hydroxysteroid dehydrogenase (NADP+) activity")|go:"GO:0102339"(3-oxo-arachidoyl-CoA reductase activity)|go:"GO:0102340"(3-oxo-behenoyl-CoA reductase activity)|go:"GO:0102341"(3-oxo-lignoceroyl-CoA reductase activity)|go:"GO:0102342"(3-oxo-cerotoyl-CoA reductase activity)|interpro:IPR002347(Glucose/ribitol dehydrogenase)|interpro:IPR020904|interpro:IPR036291|mint:O70503|reactome:R-MMU-193048|reactome:R-MMU-75876	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:6sMp3lDLISb5O1Mr9y93ntq4QJI10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P70333	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-4282223|ensembl:ENSMUSP00000050838|ensembl:ENSMUSP00000074483|ensembl:ENSMUSP00000108827|ensembl:ENSMUSP00000108828|ensembl:ENSMUSP00000108829|uniprotkb:Q3THV9	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:hnrh2_mouse(display_long)|uniprotkb:Hnrnph2(gene name)|psi-mi:Hnrnph2(display_short)|uniprotkb:Hnrph2(gene name synonym)|uniprotkb:Heterogeneous nuclear ribonucleoprotein H'(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001300645.1|refseq:NP_001300646.1|uniprotkb:A2BDV8(uniprot-removed-ac)|ensembl:ENSMUST00000074950(transcript)|ensembl:ENSMUST00000113202(transcript)|ensembl:ENSMUST00000113203(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043484"(regulation of RNA splicing)|go:"GO:1990904"(ribonucleoprotein complex)|interpro:IPR000504(RNA recognition motif, RNP-1)|interpro:IPR012677(Nucleotide-binding, alpha-beta plait)|interpro:IPR012996(Zinc finger, CHHC-type)|interpro:IPR035979|mint:P70333|reactome:R-MMU-72163|reactome:R-MMU-72203|ensembl:ENSMUST00000059297(transcript)|ensembl:ENSMUSG00000045427(gene)|ensembl:ENSMUST00000050331(transcript)|refseq:NP_063921.1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:BKLnU/p+g6hd6joRgjXU+BO5i7o10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q60668	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-299932|ensembl:ENSMUSP00000132735|uniprotkb:Q91X94|uniprotkb:Q60667|uniprotkb:Q80ZJ0	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:hnrpd_mouse(display_long)|uniprotkb:Hnrnpd(gene name)|psi-mi:Hnrnpd(display_short)|uniprotkb:Auf1(gene name synonym)|uniprotkb:Hnrpd(gene name synonym)|uniprotkb:AU-rich element RNA-binding protein 1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000000568(gene)|ensembl:ENSMUST00000172361(transcript)|go:"GO:0001889"(liver development)|go:"GO:0003680"(minor groove of adenine-thymine-rich DNA binding)|go:"GO:0003682"(chromatin binding)|go:"GO:0003723"(RNA binding)|go:"GO:0003729"(mRNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0010468"(regulation of gene expression)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0010629"(negative regulation of gene expression)|go:"GO:0021549"(cerebellum development)|go:"GO:0032204"(regulation of telomere maintenance)|go:"GO:0035925"(mRNA 3'-UTR AU-rich region binding)|go:"GO:0042162"(telomeric DNA binding)|go:"GO:0042789"(mRNA transcription by RNA polymerase II)|go:"GO:0042826"(histone deacetylase binding)|go:"GO:0043488"(regulation of mRNA stability)|go:"GO:0045202"(synapse)|go:"GO:0045727"(positive regulation of translation)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0048255"(mRNA stabilization)|go:"GO:0051592"(response to calcium ion)|go:"GO:0051602"(response to electrical stimulus)|go:"GO:0061158"(3'-UTR-mediated mRNA destabilization)|go:"GO:0071230"(cellular response to amino acid stimulus)|go:"GO:0071392"(cellular response to estradiol stimulus)|go:"GO:0071732"(cellular response to nitric oxide)|go:"GO:0097167"(circadian regulation of translation)|go:"GO:1901355"(response to rapamycin)|go:"GO:1904355"(positive regulation of telomere capping)|go:"GO:1904383"(response to sodium phosphate)|go:"GO:1904586"(cellular response to putrescine)|go:"GO:1905663"(positive regulation of telomerase RNA reverse transcriptase activity)|go:"GO:1990828"(hepatocyte dedifferentiation)|go:"GO:1990904"(ribonucleoprotein complex)|interpro:IPR000504(RNA recognition motif, RNP-1)|interpro:IPR012677(Nucleotide-binding, alpha-beta plait)|interpro:IPR012956(CBF, N-terminal)|interpro:IPR035979|mint:Q60668|reactome:R-MMU-450408|reactome:R-MMU-72163|reactome:R-MMU-72203|go:"GO:0042752"(regulation of circadian rhythm)|refseq:NP_001070733.1|refseq:NP_001070734.1|refseq:NP_001070735.1|refseq:NP_031542.2|refseq:XP_006534798.1|dip:DIP-31411N	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:lGvmHnn59pHQTtZq6H6ijlvXvt010090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q80WM4	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-26595912|ensembl:ENSMUSP00000007738|uniprotkb:Q05AB1|uniprotkb:Q80XX2	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:hpln4_mouse(display_long)|uniprotkb:Brain link protein 2(gene name synonym)|uniprotkb:Hapln4(gene name)|psi-mi:Hapln4(display_short)|uniprotkb:Bral2(gene name synonym)|uniprotkb:Lpr4(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000007594(gene)|ensembl:ENSMUST00000007738(transcript)|go:"GO:0001501"(skeletal system development)|go:"GO:0005540"(hyaluronic acid binding)|go:"GO:0005576"(extracellular region)|go:"GO:0007155"(cell adhesion)|go:"GO:0007417"(central nervous system development)|go:"GO:0031012"(extracellular matrix)|interpro:IPR000538(Link)|interpro:IPR003598(Immunoglobulin subtype 2)|interpro:IPR003599(Immunoglobulin subtype)|interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013106(Immunoglobulin V-set)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR016186(C-type lectin-like)|interpro:IPR016187(C-type lectin fold)|interpro:IPR036179|mint:Q80WM4|refseq:NP_808568.1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:+ZDITL2JE/bVUlhaU6NNpS9egLc10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9ESM3	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-26595886|ensembl:ENSMUSP00000005014	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:hpln2_mouse(display_long)|uniprotkb:Brain link protein 1(gene name synonym)|uniprotkb:Hapln2(gene name)|psi-mi:Hapln2(display_short)|uniprotkb:Bral1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000004894(gene)|ensembl:ENSMUST00000005014(transcript)|go:"GO:0001501"(skeletal system development)|go:"GO:0005540"(hyaluronic acid binding)|go:"GO:0005576"(extracellular region)|go:"GO:0007155"(cell adhesion)|go:"GO:0007417"(central nervous system development)|go:"GO:0008065"(establishment of blood-nerve barrier)|go:"GO:0031012"(extracellular matrix)|go:"GO:0085029"(extracellular matrix assembly)|interpro:IPR000538(Link)|interpro:IPR003598(Immunoglobulin subtype 2)|interpro:IPR003599(Immunoglobulin subtype)|interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013106(Immunoglobulin V-set)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR016186(C-type lectin-like)|interpro:IPR016187(C-type lectin fold)|interpro:IPR036179|mint:Q9ESM3|refseq:NP_071314.1|refseq:XP_006502211.1	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:treJqq7VkuFDDK/f1v2Gzmqp7XY10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q9QUP5	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-774834|uniprotkb:Q9D1G9|uniprotkb:Q9Z1X7|ensembl:ENSMUSP00000022108	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:hpln1_mouse(display_long)|uniprotkb:Proteoglycan link protein(gene name synonym)|uniprotkb:Hapln1(gene name)|psi-mi:Hapln1(display_short)|uniprotkb:Crtl1(gene name synonym)|uniprotkb:Cartilage-linking protein 1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_038528.3|ensembl:ENSMUSG00000021613(gene)|ensembl:ENSMUST00000022108(transcript)|go:"GO:0001501"(skeletal system development)|go:"GO:0005540"(hyaluronic acid binding)|go:"GO:0005576"(extracellular region)|go:"GO:0007155"(cell adhesion)|go:"GO:0007417"(central nervous system development)|go:"GO:0031012"(extracellular matrix)|go:"GO:0045202"(synapse)|go:"GO:0062023"(collagen-containing extracellular matrix)|interpro:IPR000538(Link)|interpro:IPR003599(Immunoglobulin subtype)|interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013106(Immunoglobulin V-set)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR016186(C-type lectin-like)|interpro:IPR016187(C-type lectin fold)|interpro:IPR036179|mint:Q9QUP5|reactome:R-MMU-3000178	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:yB6tziUt1PVpuriKEhyJrHeRVBw10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q8BH60	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-296357|uniprotkb:Q8BSV4|uniprotkb:Q920R1|uniprotkb:Q9ET11	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:gopc_mouse(display_long)|uniprotkb:Gopc(gene name)|psi-mi:Gopc(display_short)|uniprotkb:PDZ protein interacting specifically with TC10(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	go:"GO:0000139"(Golgi membrane)|go:"GO:0005109"(frizzled binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005886"(plasma membrane)|go:"GO:0006914"(autophagy)|go:"GO:0007289"(spermatid nucleus differentiation)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0010360"(negative regulation of anion channel activity)|go:"GO:0014069"(postsynaptic density)|go:"GO:0015031"(protein transport)|go:"GO:0016020"(membrane)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030140"(trans-Golgi network transport vesicle)|go:"GO:0030425"(dendrite)|go:"GO:0030695"(GTPase regulator activity)|go:"GO:0032991"(protein-containing complex)|go:"GO:0042802"(identical protein binding)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0043004"(cytoplasmic sequestering of CFTR protein)|go:"GO:0044325"(transmembrane transporter binding)|go:"GO:0045202"(synapse)|go:"GO:2000009"(negative regulation of protein localization to cell surface)|reactome:R-MMU-5627083|reactome:R-MMU-9013406|refseq:NP_001186201.1|refseq:NP_444417.2|interpro:IPR001478(PDZ/DHR/GLGF)|interpro:IPR038879|mint:Q8BH60|interpro:IPR036034	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:g5t7Tcg8R/HEVPryIgPE21/mCsA10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:P26443	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-773329|uniprotkb:Q8C273|ensembl:ENSMUSP00000022322	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:dhe3_mouse(display_long)|uniprotkb:Glud1(gene name)|psi-mi:Glud1(display_short)|uniprotkb:Glud(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26595802|imex:IM-28644-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_032159.1|ensembl:ENSMUSG00000021794(gene)|ensembl:ENSMUST00000022322(transcript)|go:"GO:0004352"("glutamate dehydrogenase (NAD+) activity")|go:"GO:0004353"("glutamate dehydrogenase [NAD(P)+] activity")|go:"GO:0004354"("glutamate dehydrogenase (NADP+) activity")|go:"GO:0005524"(ATP binding)|go:"GO:0005525"(GTP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005759"(mitochondrial matrix)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0006537"(glutamate biosynthetic process)|go:"GO:0006538"(glutamate catabolic process)|go:"GO:0006541"(glutamine metabolic process)|go:"GO:0007616"(long-term memory)|go:"GO:0010044"(response to aluminum ion)|go:"GO:0019899"(enzyme binding)|go:"GO:0021549"(cerebellum development)|go:"GO:0032024"(positive regulation of insulin secretion)|go:"GO:0043531"(ADP binding)|go:"GO:0070403"(NAD+ binding)|go:"GO:0070728"(leucine binding)|go:"GO:0072350"(tricarboxylic acid metabolic process)|interpro:IPR006095(Glutamate/phenylalanine/leucine/valine dehydrogenase)|interpro:IPR006096(Glutamate/phenylalanine/leucine/valine dehydrogenase, C-terminal)|interpro:IPR006097(Glutamate/phenylalanine/leucine/valine dehydrogenase, dimerisation region)|interpro:IPR033524|interpro:IPR033922|interpro:IPR036291|mint:P26443|reactome:R-MMU-2151201|reactome:R-MMU-8964539	-	-	-	figure legend:suppl data 6b; fig. 4|comment:"We performed ten unique immunoprecipitations (IPs) from mouse brain lysates using control IgGs, AIDA-1 IgGs, and antibodies to unrelated synaptic proteins crosslinked to protein G-agarose or magnetic beads. Each eluted IP sample was labeled with a unique isobaric tag within a 10-plex set of tandem mass tags43. This approach allowed us to accurately quantify identified peptides, simultaneously analyze all samples to reduce variability associated with mass spectrometry (MS) analysis, and rigorously control for nonspecific binding by normalizing to control IgG samples and comparing to unrelated protein interactomes (Supplementary Data 6a). We used a commercially available antibody (C-10, Santa Cruz Biotechnologies), as well as in-house antibodies known to immunoprecipitate AIDA-1 (1A11 and 2B22) (Fig. 4b). We defined the AIDA-1 interactome as proteins co-immunoprecipitated by AIDA-1 antibodies that were identified and quantified by ≥3 peptides and enriched >1.4-fold over background in agarose experiments, or >3.0-fold in magnetic bead experiments (Supplementary Data 6b)."|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:HbPeUm/tpMD6GrgM/863swTQqGA10090	rigid:Hcn7DZgTnV/ptVajo6zjCO73/qY	false	-	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q8BIZ1	uniprotkb:Q91Z67	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-16730393|ensembl:ENSMUSP00000095195|uniprotkb:B2RY13|uniprotkb:Q3V1V8|uniprotkb:Q61054	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:srgp2_mouse(display_long)|uniprotkb:Formin-binding protein 2(gene name synonym)|uniprotkb:Formin-binding protein 27(gene name synonym)|uniprotkb:Srgap2(gene name)|psi-mi:Srgap2(display_short)|uniprotkb:Fbp27(gene name synonym)|uniprotkb:Fnbp2(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26596255|imex:IM-28644-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	ensembl:ENSMUSG00000026425(gene)|ensembl:ENSMUST00000097588(transcript)|go:"GO:0003363"(lamellipodium assembly involved in ameboidal cell migration)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0007165"(signal transduction)|go:"GO:0014069"(postsynaptic density)|go:"GO:0021816"(extension of a leading process involved in cell motility in cerebral cortex radial glia guided migration)|go:"GO:0030027"(lamellipodium)|go:"GO:0030336"(negative regulation of cell migration)|go:"GO:0031267"(small GTPase binding)|go:"GO:0034446"(substrate adhesion-dependent cell spreading)|go:"GO:0042802"(identical protein binding)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0043547"(positive regulation of GTPase activity)|go:"GO:0044327"(dendritic spine head)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0045335"(phagocytic vesicle)|go:"GO:0046847"(filopodium assembly)|go:"GO:0048812"(neuron projection morphogenesis)|go:"GO:0051014"(actin filament severing)|go:"GO:0060996"(dendritic spine development)|go:"GO:2001223"(negative regulation of neuron migration)|interpro:IPR000198(RhoGAP)|interpro:IPR001060(Cdc15/Fes/CIP4)|interpro:IPR001452(Src homology-3)|interpro:IPR008936(Rho GTPase activation protein)|interpro:IPR027267|interpro:IPR030252|interpro:IPR031160|interpro:IPR035648|interpro:IPR036028|mint:Q91Z67|reactome:R-MMU-5663220|reactome:R-MMU-9013148|reactome:R-MMU-9013149|reactome:R-MMU-9013406|reactome:R-MMU-9013420|reactome:R-MMU-9013423|reactome:R-MMU-9035034|refseq:NP_001074480.2|refseq:XP_006529197.1	-	-	-	figure legend:fig. 4f|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:MLCAa8wEQvKNPatehgSzcizs2YI10090	rigid:t5tJGFlVGKuBvzF6Q5FYizAuz6w	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q8BIZ1	uniprotkb:Q9QWY8	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-698498|uniprotkb:Q99LV8|uniprotkb:Q9Z2B6|uniprotkb:O08612|uniprotkb:Q80TG8|uniprotkb:Q80UV6|uniprotkb:Q505F0|ensembl:ENSMUSP00000134825	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:asap1_mouse(display_long)|uniprotkb:PIP2-dependent ARF1 GAP(gene name synonym)|uniprotkb:ADP-ribosylation factor-directed GTPase-activating protein 1(gene name synonym)|uniprotkb:Development and differentiation-enhancing factor 1(gene name synonym)|uniprotkb:Asap1(gene name)|psi-mi:Asap1(display_short)|uniprotkb:Ddef1(gene name synonym)|uniprotkb:Kiaa1249(gene name synonym)|uniprotkb:Shag1(gene name synonym)|uniprotkb:130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26596255|imex:IM-28644-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001263396.1|refseq:NP_034156.2|refseq:NP_001263390.1|refseq:NP_001263391.1|ensembl:ENSMUSG00000022377(gene)|ensembl:ENSMUST00000177374(transcript)|go:"GO:0001786"(phosphatidylserine binding)|go:"GO:0002102"(podosome)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005546"(phosphatidylinositol-4,5-bisphosphate binding)|go:"GO:0005547"(phosphatidylinositol-3,4,5-trisphosphate binding)|go:"GO:0005829"(cytosol)|go:"GO:0031253"(cell projection membrane)|go:"GO:0043197"(dendritic spine)|go:"GO:0043547"(positive regulation of GTPase activity)|go:"GO:0046872"(metal ion binding)|go:"GO:0060271"(cilium assembly)|go:"GO:0061000"(negative regulation of dendritic spine development)|go:"GO:0071803"(positive regulation of podosome assembly)|go:"GO:1903527"(positive regulation of membrane tubulation)|interpro:IPR001164(Arf GTPase activating protein)|interpro:IPR001452(Src homology-3)|interpro:IPR001849(Pleckstrin-like)|interpro:IPR002110(Ankyrin)|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR020683|interpro:IPR027267|interpro:IPR036028|interpro:IPR036770|interpro:IPR037278|interpro:IPR037844|interpro:IPR037928|interpro:IPR038016|interpro:IPR038508|interpro:IPR043593|mint:Q9QWY8|rcsb pdb:5C6R|rcsb pdb:5C79|reactome:R-MMU-5620916	-	-	-	figure legend:fig. 4f|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:/jhMt+/t+DTqI3ZQmzU0IMFCHdA10090	rigid:t5tJGFlVGKuBvzF6Q5FYizAuz6w	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q8BIZ1	uniprotkb:Q9Z0R4	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-645386|uniprotkb:Q9R143|uniprotkb:F8VQE5|ensembl:ENSMUSP00000109635	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:itsn1_mouse(display_long)|uniprotkb:EH and SH3 domains protein 1(gene name synonym)|uniprotkb:Itsn1(gene name)|psi-mi:Itsn1(display_short)|uniprotkb:Ese1(gene name synonym)|uniprotkb:Itsn(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26596255|imex:IM-28644-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001103745.1|refseq:NP_034717.2|ensembl:ENSMUSG00000022957(gene)|ensembl:ENSMUST00000114002(transcript)|go:"GO:0005085"(guanyl-nucleotide exchange factor activity)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005635"(nuclear envelope)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0006887"(exocytosis)|go:"GO:0006897"(endocytosis)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0015031"(protein transport)|go:"GO:0016197"(endosomal transport)|go:"GO:0019209"(kinase activator activity)|go:"GO:0030027"(lamellipodium)|go:"GO:0030139"(endocytic vesicle)|go:"GO:0034613"(cellular protein localization)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043197"(dendritic spine)|go:"GO:0043524"(negative regulation of neuron apoptotic process)|go:"GO:0044305"(calyx of Held)|go:"GO:0045202"(synapse)|go:"GO:0048488"(synaptic vesicle endocytosis)|go:"GO:0051897"(positive regulation of protein kinase B signaling)|go:"GO:0055037"(recycling endosome)|go:"GO:0060090"(molecular adaptor activity)|go:"GO:0060124"(positive regulation of growth hormone secretion)|go:"GO:0060999"(positive regulation of dendritic spine development)|go:"GO:0070064"(proline-rich region binding)|go:"GO:0097440"(apical dendrite)|go:"GO:0097708"(intracellular vesicle)|go:"GO:0098833"(presynaptic endocytic zone)|go:"GO:0098871"(postsynaptic actin cytoskeleton)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0150007"(clathrin-dependent synaptic vesicle endocytosis)|go:"GO:1905274"(regulation of modification of postsynaptic actin cytoskeleton)|go:"GO:2001288"(positive regulation of caveolin-mediated endocytosis)|interpro:IPR000008(C2 calcium-dependent membrane targeting)|interpro:IPR000219("Dbl homology (DH) domain")|interpro:IPR000261("EPS15 homology (EH)")|interpro:IPR001331(Guanine-nucleotide dissociation stimulator, CDC24, conserved site)|interpro:IPR001452(Src homology-3)|interpro:IPR001849(Pleckstrin-like)|interpro:IPR002048(Calcium-binding EF-hand)|interpro:IPR011992(EF-Hand type)|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR018247|interpro:IPR035892|interpro:IPR035899|interpro:IPR036028|mint:Q9Z0R4|rcsb pdb:3HS8|rcsb pdb:3JV3|reactome:R-MMU-193648|reactome:R-MMU-3928662|reactome:R-MMU-416482|reactome:R-MMU-8856825|reactome:R-MMU-8856828|reactome:R-MMU-9013148|reactome:R-MMU-9013406|reactome:R-MMU-9013408	-	-	-	figure legend:fig. 4f|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:naFu4D6P1S+/t5+VVVeb0De8lEg10090	rigid:t5tJGFlVGKuBvzF6Q5FYizAuz6w	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q8BIZ1	uniprotkb:Q68FF6	intact:EBI-16724439|uniprotkb:E9Q644|uniprotkb:Q4KMR9|uniprotkb:Q8BJ47|uniprotkb:Q8BJ49	intact:EBI-645933|ensembl:ENSMUSP00000037210	psi-mi:ans1b_mouse(display_long)|uniprotkb:Amyloid-beta protein intracellular domain-associated protein 1(gene name synonym)|uniprotkb:E2A-PBX1-associated protein(gene name synonym)|uniprotkb:Anks1b(gene name)|psi-mi:Anks1b(display_short)	psi-mi:git1_mouse(display_long)|uniprotkb:G protein-coupled receptor kinase-interactor 1(gene name synonym)|uniprotkb:GRK-interacting protein 1(gene name synonym)|uniprotkb:Git1(gene name)|psi-mi:Git1(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Carbonell et al. (2019)	pubmed:31388001|imex:IM-28644	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26596255|imex:IM-28644-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR041880|interpro:IPR041882|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0014069"(postsynaptic density)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0046875"(ephrin receptor binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099092"(postsynaptic density, intracellular component)|go:"GO:0099523"(presynaptic cytosol)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:1900383"(regulation of synaptic plasticity by receptor localization to synapse)|mint:Q8BIZ1|refseq:NP_001170868.1|refseq:NP_001170869.1|refseq:NP_001333982.1|refseq:NP_001333983.1|refseq:NP_852063.1|interpro:IPR033636|interpro:IPR002110(Ankyrin)|interpro:IPR020683|interpro:IPR036770|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR006020(Phosphotyrosine interaction region)|interpro:IPR001660(Sterile alpha motif SAM)|interpro:IPR013761(Sterile alpha motif-type)	refseq:NP_001004144.1|ensembl:ENSMUSG00000011877(gene)|ensembl:ENSMUST00000037285(transcript)|go:"GO:0001771"(immunological synapse formation)|go:"GO:0001957"(intramembranous ossification)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0005768"(endosome)|go:"GO:0005813"(centrosome)|go:"GO:0005829"(cytosol)|go:"GO:0005925"(focal adhesion)|go:"GO:0007420"(brain development)|go:"GO:0007626"(locomotory behavior)|go:"GO:0008277"(regulation of G protein-coupled receptor signaling pathway)|go:"GO:0014069"(postsynaptic density)|go:"GO:0030027"(lamellipodium)|go:"GO:0031267"(small GTPase binding)|go:"GO:0032013"(negative regulation of ARF protein signal transduction)|go:"GO:0032465"(regulation of cytokinesis)|go:"GO:0032691"(negative regulation of interleukin-1 beta production)|go:"GO:0042802"(identical protein binding)|go:"GO:0043005"(neuron projection)|go:"GO:0043015"(gamma-tubulin binding)|go:"GO:0044305"(calyx of Held)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045202"(synapse)|go:"GO:0045454"(cell redox homeostasis)|go:"GO:0045820"(negative regulation of glycolytic process)|go:"GO:0046872"(metal ion binding)|go:"GO:0048013"(ephrin receptor signaling pathway)|go:"GO:0048666"(neuron development)|go:"GO:0060996"(dendritic spine development)|go:"GO:0061743"(motor learning)|go:"GO:0071222"(cellular response to lipopolysaccharide)|go:"GO:0071364"(cellular response to epidermal growth factor stimulus)|go:"GO:0090063"(positive regulation of microtubule nucleation)|go:"GO:0097431"(mitotic spindle pole)|go:"GO:0098794"(postsynapse)|go:"GO:0098879"(structural constituent of postsynaptic specialization)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099171"(presynaptic modulation of chemical synaptic transmission)|go:"GO:0099645"(neurotransmitter receptor localization to postsynaptic specialization membrane)|go:"GO:0106015"(negative regulation of inflammatory response to wounding)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|go:"GO:2000646"(positive regulation of receptor catabolic process)|interpro:IPR001164(Arf GTPase activating protein)|interpro:IPR002110(Ankyrin)|interpro:IPR013724("Spa2 homology (SHD) of GIT")|interpro:IPR020683|interpro:IPR022018|interpro:IPR032352|interpro:IPR036770|interpro:IPR037278|interpro:IPR038508|mint:Q68FF6|rcsb pdb:6JMU|reactome:R-MMU-3928664|reactome:R-MMU-9013148|reactome:R-MMU-9013149|reactome:R-MMU-9013404|reactome:R-MMU-9013406|reactome:R-MMU-9013420|reactome:R-MMU-9013423|reactome:R-MMU-9013424	-	-	-	figure legend:fig. 4f|dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-brain)|taxid:10090(Mus musculus brain)	-	2021/01/18	2021/01/28	rogid:xm2OG9kb/tmIo7e7c/qXBE4JeAY10090	rogid:3br9Xz6GZQV409u3u7CZDfqKMIM10090	rigid:t5tJGFlVGKuBvzF6Q5FYizAuz6w	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)