#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P35414	uniprotkb:P32121	intact:EBI-2875891|ensembl:ENSP00000257254|ensembl:ENSP00000475344	intact:EBI-714559|uniprotkb:Q0Z8D3|uniprotkb:Q2PP19|uniprotkb:Q6ICT3|uniprotkb:Q8N7Y2|uniprotkb:Q9UEQ6|uniprotkb:B7WPL3|uniprotkb:B5B0C0|uniprotkb:D3DTK2|intact:EBI-28974065|ensembl:ENSP00000269260|uniprotkb:B4DLW0|uniprotkb:H0Y688	psi-mi:apj_human(display_long)|uniprotkb:APLNR(gene name)|psi-mi:APLNR(display_short)|uniprotkb:AGTRL1(gene name synonym)|uniprotkb:APJ(gene name synonym)|uniprotkb:Angiotensin receptor-like 1(gene name synonym)|uniprotkb:G-protein coupled receptor APJ(gene name synonym)|uniprotkb:G-protein coupled receptor HG11(gene name synonym)	psi-mi:arrb2_human(display_long)|uniprotkb:Arrestin beta-2(gene name synonym)|uniprotkb:ARRB2(gene name)|psi-mi:ARRB2(display_short)|uniprotkb:ARB2(gene name synonym)|uniprotkb:ARR2(gene name synonym)|uniprotkb:Non-visual arrestin-3(gene name synonym)	psi-mi:"MI:1203"(split luciferase complementation)	Ban et al. (2018)	pubmed:30409826|imex:IM-26947	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-21298405|imex:IM-26947-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensembl:ENSG00000134817(gene)|ensembl:ENST00000257254(transcript)|ensembl:ENST00000606794(transcript)|go:"GO:0001525"(angiogenesis)|go:"GO:0001568"(blood vessel development)|go:"GO:0001570"(vasculogenesis)|go:"GO:0001944"(vasculature development)|go:"GO:0001947"(heart looping)|go:"GO:0003171"(atrioventricular valve development)|go:"GO:0003272"(endocardial cushion formation)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007369"(gastrulation)|go:"GO:0007507"(heart development)|go:"GO:0007512"(adult heart development)|go:"GO:0010468"(regulation of gene expression)|go:"GO:0010629"(negative regulation of gene expression)|go:"GO:0031065"(positive regulation of histone deacetylation)|go:"GO:0035886"(vascular associated smooth muscle cell differentiation)|go:"GO:0035904"(aorta development)|go:"GO:0038023"(signaling receptor activity)|go:"GO:0043951"(negative regulation of cAMP-mediated signaling)|go:"GO:0045766"(positive regulation of angiogenesis)|go:"GO:0050878"(regulation of body fluid levels)|go:"GO:0051281"(positive regulation of release of sequestered calcium ion into cytosol)|go:"GO:0060182"(apelin receptor activity)|go:"GO:0060183"(apelin receptor signaling pathway)|go:"GO:0060412"(ventricular septum morphogenesis)|go:"GO:0060841"(venous blood vessel development)|go:"GO:0060976"(coronary vasculature development)|go:"GO:1903589"(positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis)|go:"GO:1903596"(regulation of gap junction assembly)|go:"GO:1904325"(positive regulation of inhibitory G protein-coupled receptor phosphorylation)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR003904(APJ receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|mint:P35414|rcsb pdb:2LOT|rcsb pdb:2LOU|rcsb pdb:2LOV|rcsb pdb:2LOW|rcsb pdb:5VBL|rcsb pdb:6KNM|reactome:R-HSA-375276|reactome:R-HSA-418594|refseq:NP_005152.1	ensembl:ENSP00000269260|ensembl:ENST00000346341|ensembl:ENSP00000341895|ensembl:ENST00000381488|ensembl:ENSP00000370898|ensembl:ENST00000412477|ensembl:ENSP00000403701|ensembl:ENSG00000141480(gene)|ensembl:ENST00000269260(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0002031"(G protein-coupled receptor internalization)|go:"GO:0002032"(desensitization of G protein-coupled receptor signaling pathway by arrestin)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0005102"(signaling receptor binding)|interpro:IPR017864|go:"GO:0005634"(nucleus)|mint:P32121|reactome:R-HSA-2122948|reactome:R-HSA-418555|reactome:R-HSA-456926|reactome:R-HSA-5099900|reactome:R-HSA-5635838|reactome:R-HSA-5674135|reactome:R-HSA-5689880|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-8856825|reactome:R-HSA-8856828|reactome:R-HSA-9649948|reactome:R-HSA-9656223|interpro:IPR014756(Immunoglobulin E-set)|go:"GO:0016323"(basolateral plasma membrane)|go:"GO:0016567"(protein ubiquitination)|go:"GO:0019899"(enzyme binding)|go:"GO:0019904"(protein domain specific binding)|go:"GO:0030139"(endocytic vesicle)|go:"GO:0031397"(negative regulation of protein ubiquitination)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031623"(receptor internalization)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0031691"(alpha-1A adrenergic receptor binding)|go:"GO:0031692"(alpha-1B adrenergic receptor binding)|go:"GO:0031701"(angiotensin receptor binding)|go:"GO:0031702"(type 1 angiotensin receptor binding)|go:"GO:0031748"(D1 dopamine receptor binding)|go:"GO:0031762"(follicle-stimulating hormone receptor binding)|go:"GO:0031826"(type 2A serotonin receptor binding)|go:"GO:0031859"(platelet activating factor receptor binding)|go:"GO:0032088"(negative regulation of NF-kappaB transcription factor activity)|go:"GO:0032226"(positive regulation of synaptic transmission, dopaminergic)|go:"GO:0032691"(negative regulation of interleukin-1 beta production)|go:"GO:0032695"(negative regulation of interleukin-12 production)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005768"(endosome)|go:"GO:0032715"(negative regulation of interleukin-6 production)|go:"GO:0032720"(negative regulation of tumor necrosis factor production)|go:"GO:0032967"(positive regulation of collagen biosynthetic process)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|go:"GO:0034122"(negative regulation of toll-like receptor signaling pathway)|go:"GO:0034260"(negative regulation of GTPase activity)|go:"GO:0034392"(negative regulation of smooth muscle cell apoptotic process)|go:"GO:0042699"(follicle-stimulating hormone signaling pathway)|go:"GO:0042802"(identical protein binding)|go:"GO:0005829"(cytosol)|go:"GO:0043154"(negative regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0043161"(proteasome-mediated ubiquitin-dependent protein catabolic process)|go:"GO:0043197"(dendritic spine)|go:"GO:0043422"(protein kinase B binding)|go:"GO:0043524"(negative regulation of neuron apoptotic process)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0045953"(negative regulation of natural killer cell mediated cytotoxicity)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050965"(detection of temperature stimulus involved in sensory perception of pain)|go:"GO:0005886"(plasma membrane)|go:"GO:0051019"(mitogen-activated protein kinase binding)|go:"GO:0051897"(positive regulation of protein kinase B signaling)|go:"GO:0051898"(negative regulation of protein kinase B signaling)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0006366"(transcription by RNA polymerase II)|go:"GO:0051928"(positive regulation of calcium ion transport)|go:"GO:0060079"(excitatory postsynaptic potential)|go:"GO:0007179"(transforming growth factor beta receptor signaling pathway)|go:"GO:0060326"(cell chemotaxis)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071889"(14-3-3 protein binding)|go:"GO:0090201"(negative regulation of release of cytochrome c from mitochondria)|go:"GO:1904037"(positive regulation of epithelial cell apoptotic process)|go:"GO:1990763"(arrestin family protein binding)|go:"GO:2000573"(positive regulation of DNA biosynthetic process)|go:"GO:2000727"(positive regulation of cardiac muscle cell differentiation)|go:"GO:0007212"(dopamine receptor signaling pathway)|interpro:IPR000698(Arrestin)|interpro:IPR011021(Arrestin-like, N-terminal)|interpro:IPR011022(Arrestin-like, C-terminal)|go:"GO:0007420"(brain development)|interpro:IPR014752(Arrestin, C-terminal)|interpro:IPR014753(Arrestin, N-terminal)|go:"GO:0007628"(adult walking behavior)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0014069"(postsynaptic density)|go:"GO:0015031"(protein transport)|refseq:NP_004304.1|refseq:NP_945355.1|refseq:NP_001316993.1|refseq:NP_001244259.1|refseq:NP_001244260.1|refseq:NP_001244257.1|refseq:NP_001244258.1|dip:DIP-40089N	-	-	-	figure legend:Figure 3A|agonist:Apelin-13 peptide|comment:"Agonist AP-13 stimulation induced a rapid and robust signal increase.  Under the same conditions, the APJ I109A mutant completely abolished AP-13 stimulated beta-arrestin recruitment (Fig. 3A)"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293ft)|taxid:9606(Homo sapiens HEK293FT cell line)	-	2019/03/29	2019/04/05	rogid:OaKR4qnsU9jANK+HB2qQ4TlanpQ9606	rogid:MY34flMH9cdhPtMLGYOQtoQyr2U9606	rigid:GAu6MgEzlELEpQSMgUf30f41CW0	false	luciferase tag:c-c	luciferase tag:n-n	-	-	psi-mi:"MI:1220"(tag visualisation by luciferase assay)	psi-mi:"MI:1220"(tag visualisation by luciferase assay)
uniprotkb:P35414	uniprotkb:P25098	intact:EBI-2875891|ensembl:ENSP00000257254|ensembl:ENSP00000475344	intact:EBI-3904795|uniprotkb:Q6GTT3|uniprotkb:B0ZBE1|uniprotkb:Q13837|ensembl:ENSP00000312262	psi-mi:apj_human(display_long)|uniprotkb:APLNR(gene name)|psi-mi:APLNR(display_short)|uniprotkb:AGTRL1(gene name synonym)|uniprotkb:APJ(gene name synonym)|uniprotkb:Angiotensin receptor-like 1(gene name synonym)|uniprotkb:G-protein coupled receptor APJ(gene name synonym)|uniprotkb:G-protein coupled receptor HG11(gene name synonym)	psi-mi:arbk1_human(display_long)|uniprotkb:GRK2(gene name)|psi-mi:GRK2(display_short)|uniprotkb:ADRBK1(gene name synonym)|uniprotkb:BARK(gene name synonym)|uniprotkb:BARK1(gene name synonym)|uniprotkb:G-protein coupled receptor kinase 2(gene name synonym)	psi-mi:"MI:0012"(bioluminescence resonance energy transfer)	Ban et al. (2018)	pubmed:30409826|imex:IM-26947	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:2364"(proximity)	psi-mi:"MI:0469"(IntAct)	intact:EBI-21298471|imex:IM-26947-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0584"(fluorescence acceptor)	psi-mi:"MI:0583"(fluorescence donor)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensembl:ENSG00000134817(gene)|ensembl:ENST00000257254(transcript)|ensembl:ENST00000606794(transcript)|go:"GO:0001525"(angiogenesis)|go:"GO:0001568"(blood vessel development)|go:"GO:0001570"(vasculogenesis)|go:"GO:0001944"(vasculature development)|go:"GO:0001947"(heart looping)|go:"GO:0003171"(atrioventricular valve development)|go:"GO:0003272"(endocardial cushion formation)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007369"(gastrulation)|go:"GO:0007507"(heart development)|go:"GO:0007512"(adult heart development)|go:"GO:0010468"(regulation of gene expression)|go:"GO:0010629"(negative regulation of gene expression)|go:"GO:0031065"(positive regulation of histone deacetylation)|go:"GO:0035886"(vascular associated smooth muscle cell differentiation)|go:"GO:0035904"(aorta development)|go:"GO:0038023"(signaling receptor activity)|go:"GO:0043951"(negative regulation of cAMP-mediated signaling)|go:"GO:0045766"(positive regulation of angiogenesis)|go:"GO:0050878"(regulation of body fluid levels)|go:"GO:0051281"(positive regulation of release of sequestered calcium ion into cytosol)|go:"GO:0060182"(apelin receptor activity)|go:"GO:0060183"(apelin receptor signaling pathway)|go:"GO:0060412"(ventricular septum morphogenesis)|go:"GO:0060841"(venous blood vessel development)|go:"GO:0060976"(coronary vasculature development)|go:"GO:1903589"(positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis)|go:"GO:1903596"(regulation of gap junction assembly)|go:"GO:1904325"(positive regulation of inhibitory G protein-coupled receptor phosphorylation)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR003904(APJ receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|mint:P35414|rcsb pdb:2LOT|rcsb pdb:2LOU|rcsb pdb:2LOV|rcsb pdb:2LOW|rcsb pdb:5VBL|rcsb pdb:6KNM|reactome:R-HSA-375276|reactome:R-HSA-418594|refseq:NP_005152.1	refseq:NP_001610.2|dip:DIP-42429N|ensembl:ENSG00000173020(gene)|ensembl:ENST00000308595(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0002026"(regulation of the force of heart contraction)|go:"GO:0002029"(desensitization of G protein-coupled receptor signaling pathway)|go:"GO:0003108"(negative regulation of the force of heart contraction by chemical signal)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004703"(G protein-coupled receptor kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005929"(cilium)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007213"(G protein-coupled acetylcholine receptor signaling pathway)|go:"GO:0007217"(tachykinin receptor signaling pathway)|go:"GO:0007507"(heart development)|go:"GO:0009966"(regulation of signal transduction)|go:"GO:0016020"(membrane)|go:"GO:0018105"(peptidyl-serine phosphorylation)|go:"GO:0018107"(peptidyl-threonine phosphorylation)|go:"GO:0019079"(viral genome replication)|go:"GO:0031623"(receptor internalization)|go:"GO:0031694"(alpha-2A adrenergic receptor binding)|go:"GO:0031755"(Edg-2 lysophosphatidic acid receptor binding)|go:"GO:0033605"(positive regulation of catecholamine secretion)|go:"GO:0005737"(cytoplasm)|go:"GO:0045988"(negative regulation of striated muscle contraction)|go:"GO:0046718"(viral entry into host cell)|go:"GO:0047696"(beta-adrenergic receptor kinase activity)|go:"GO:0060048"(cardiac muscle contraction)|go:"GO:1901081"(negative regulation of relaxation of smooth muscle)|interpro:IPR000239(GPCR kinase)|interpro:IPR000719(Protein kinase, core)|interpro:IPR000961(Protein kinase, C-terminal)|interpro:IPR001849(Pleckstrin-like)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR016137(Regulator of G protein signalling superfamily)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR036305|mint:P25098|rcsb pdb:1BAK|rcsb pdb:3CIK|rcsb pdb:3KRW|rcsb pdb:3KRX|rcsb pdb:3V5W|rcsb pdb:4MK0|rcsb pdb:4PNK|rcsb pdb:5HE1|rcsb pdb:5UKK|rcsb pdb:5UKL|rcsb pdb:5UUU|rcsb pdb:5UVC|rcsb pdb:5WG3|rcsb pdb:5WG4|rcsb pdb:5WG5|rcsb pdb:6C2Y|rcsb pdb:6U7C|rcsb pdb:7K7L|rcsb pdb:7K7Z|reactome:R-HSA-111933|reactome:R-HSA-416476|reactome:R-HSA-418555|reactome:R-HSA-5635838|reactome:R-HSA-8856825|go:"GO:0045202"(synapse)	-	-	-	figure legend:Figure 3B|agonist:Apelin-13 peptide|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293ft)|taxid:9606(Homo sapiens HEK293FT cell line)	-	2019/03/29	2019/04/05	rogid:OaKR4qnsU9jANK+HB2qQ4TlanpQ9606	rogid:iAd5kj9koCtmmVivrWfgN4y0YMc9606	rigid:a/WsZ2/h/bdGNBtvK8FHEK0YCvA	false	green fluorescent protein tag:?-?|mutation disrupting interaction strength:109-109|mutation disrupting interaction strength:168-168	renilla luciferase protein tag:?-?	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
uniprotkb:P35414	uniprotkb:P32121	intact:EBI-2875891|ensembl:ENSP00000257254|ensembl:ENSP00000475344	intact:EBI-714559|uniprotkb:Q0Z8D3|uniprotkb:Q2PP19|uniprotkb:Q6ICT3|uniprotkb:Q8N7Y2|uniprotkb:Q9UEQ6|uniprotkb:B7WPL3|uniprotkb:B5B0C0|uniprotkb:D3DTK2|intact:EBI-28974065|ensembl:ENSP00000269260|uniprotkb:B4DLW0|uniprotkb:H0Y688	psi-mi:apj_human(display_long)|uniprotkb:APLNR(gene name)|psi-mi:APLNR(display_short)|uniprotkb:AGTRL1(gene name synonym)|uniprotkb:APJ(gene name synonym)|uniprotkb:Angiotensin receptor-like 1(gene name synonym)|uniprotkb:G-protein coupled receptor APJ(gene name synonym)|uniprotkb:G-protein coupled receptor HG11(gene name synonym)	psi-mi:arrb2_human(display_long)|uniprotkb:Arrestin beta-2(gene name synonym)|uniprotkb:ARRB2(gene name)|psi-mi:ARRB2(display_short)|uniprotkb:ARB2(gene name synonym)|uniprotkb:ARR2(gene name synonym)|uniprotkb:Non-visual arrestin-3(gene name synonym)	psi-mi:"MI:0663"(confocal microscopy)	Ban et al. (2018)	pubmed:30409826|imex:IM-26947	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0403"(colocalization)	psi-mi:"MI:0469"(IntAct)	intact:EBI-21298703|imex:IM-26947-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensembl:ENSG00000134817(gene)|ensembl:ENST00000257254(transcript)|ensembl:ENST00000606794(transcript)|go:"GO:0001525"(angiogenesis)|go:"GO:0001568"(blood vessel development)|go:"GO:0001570"(vasculogenesis)|go:"GO:0001944"(vasculature development)|go:"GO:0001947"(heart looping)|go:"GO:0003171"(atrioventricular valve development)|go:"GO:0003272"(endocardial cushion formation)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007369"(gastrulation)|go:"GO:0007507"(heart development)|go:"GO:0007512"(adult heart development)|go:"GO:0010468"(regulation of gene expression)|go:"GO:0010629"(negative regulation of gene expression)|go:"GO:0031065"(positive regulation of histone deacetylation)|go:"GO:0035886"(vascular associated smooth muscle cell differentiation)|go:"GO:0035904"(aorta development)|go:"GO:0038023"(signaling receptor activity)|go:"GO:0043951"(negative regulation of cAMP-mediated signaling)|go:"GO:0045766"(positive regulation of angiogenesis)|go:"GO:0050878"(regulation of body fluid levels)|go:"GO:0051281"(positive regulation of release of sequestered calcium ion into cytosol)|go:"GO:0060182"(apelin receptor activity)|go:"GO:0060183"(apelin receptor signaling pathway)|go:"GO:0060412"(ventricular septum morphogenesis)|go:"GO:0060841"(venous blood vessel development)|go:"GO:0060976"(coronary vasculature development)|go:"GO:1903589"(positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis)|go:"GO:1903596"(regulation of gap junction assembly)|go:"GO:1904325"(positive regulation of inhibitory G protein-coupled receptor phosphorylation)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR003904(APJ receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|mint:P35414|rcsb pdb:2LOT|rcsb pdb:2LOU|rcsb pdb:2LOV|rcsb pdb:2LOW|rcsb pdb:5VBL|rcsb pdb:6KNM|reactome:R-HSA-375276|reactome:R-HSA-418594|refseq:NP_005152.1	ensembl:ENSP00000269260|ensembl:ENST00000346341|ensembl:ENSP00000341895|ensembl:ENST00000381488|ensembl:ENSP00000370898|ensembl:ENST00000412477|ensembl:ENSP00000403701|ensembl:ENSG00000141480(gene)|ensembl:ENST00000269260(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0002031"(G protein-coupled receptor internalization)|go:"GO:0002032"(desensitization of G protein-coupled receptor signaling pathway by arrestin)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0005102"(signaling receptor binding)|interpro:IPR017864|go:"GO:0005634"(nucleus)|mint:P32121|reactome:R-HSA-2122948|reactome:R-HSA-418555|reactome:R-HSA-456926|reactome:R-HSA-5099900|reactome:R-HSA-5635838|reactome:R-HSA-5674135|reactome:R-HSA-5689880|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-8856825|reactome:R-HSA-8856828|reactome:R-HSA-9649948|reactome:R-HSA-9656223|interpro:IPR014756(Immunoglobulin E-set)|go:"GO:0016323"(basolateral plasma membrane)|go:"GO:0016567"(protein ubiquitination)|go:"GO:0019899"(enzyme binding)|go:"GO:0019904"(protein domain specific binding)|go:"GO:0030139"(endocytic vesicle)|go:"GO:0031397"(negative regulation of protein ubiquitination)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031623"(receptor internalization)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0031691"(alpha-1A adrenergic receptor binding)|go:"GO:0031692"(alpha-1B adrenergic receptor binding)|go:"GO:0031701"(angiotensin receptor binding)|go:"GO:0031702"(type 1 angiotensin receptor binding)|go:"GO:0031748"(D1 dopamine receptor binding)|go:"GO:0031762"(follicle-stimulating hormone receptor binding)|go:"GO:0031826"(type 2A serotonin receptor binding)|go:"GO:0031859"(platelet activating factor receptor binding)|go:"GO:0032088"(negative regulation of NF-kappaB transcription factor activity)|go:"GO:0032226"(positive regulation of synaptic transmission, dopaminergic)|go:"GO:0032691"(negative regulation of interleukin-1 beta production)|go:"GO:0032695"(negative regulation of interleukin-12 production)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005768"(endosome)|go:"GO:0032715"(negative regulation of interleukin-6 production)|go:"GO:0032720"(negative regulation of tumor necrosis factor production)|go:"GO:0032967"(positive regulation of collagen biosynthetic process)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|go:"GO:0034122"(negative regulation of toll-like receptor signaling pathway)|go:"GO:0034260"(negative regulation of GTPase activity)|go:"GO:0034392"(negative regulation of smooth muscle cell apoptotic process)|go:"GO:0042699"(follicle-stimulating hormone signaling pathway)|go:"GO:0042802"(identical protein binding)|go:"GO:0005829"(cytosol)|go:"GO:0043154"(negative regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0043161"(proteasome-mediated ubiquitin-dependent protein catabolic process)|go:"GO:0043197"(dendritic spine)|go:"GO:0043422"(protein kinase B binding)|go:"GO:0043524"(negative regulation of neuron apoptotic process)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0045953"(negative regulation of natural killer cell mediated cytotoxicity)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050965"(detection of temperature stimulus involved in sensory perception of pain)|go:"GO:0005886"(plasma membrane)|go:"GO:0051019"(mitogen-activated protein kinase binding)|go:"GO:0051897"(positive regulation of protein kinase B signaling)|go:"GO:0051898"(negative regulation of protein kinase B signaling)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0006366"(transcription by RNA polymerase II)|go:"GO:0051928"(positive regulation of calcium ion transport)|go:"GO:0060079"(excitatory postsynaptic potential)|go:"GO:0007179"(transforming growth factor beta receptor signaling pathway)|go:"GO:0060326"(cell chemotaxis)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071889"(14-3-3 protein binding)|go:"GO:0090201"(negative regulation of release of cytochrome c from mitochondria)|go:"GO:1904037"(positive regulation of epithelial cell apoptotic process)|go:"GO:1990763"(arrestin family protein binding)|go:"GO:2000573"(positive regulation of DNA biosynthetic process)|go:"GO:2000727"(positive regulation of cardiac muscle cell differentiation)|go:"GO:0007212"(dopamine receptor signaling pathway)|interpro:IPR000698(Arrestin)|interpro:IPR011021(Arrestin-like, N-terminal)|interpro:IPR011022(Arrestin-like, C-terminal)|go:"GO:0007420"(brain development)|interpro:IPR014752(Arrestin, C-terminal)|interpro:IPR014753(Arrestin, N-terminal)|go:"GO:0007628"(adult walking behavior)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0014069"(postsynaptic density)|go:"GO:0015031"(protein transport)|refseq:NP_004304.1|refseq:NP_945355.1|refseq:NP_001316993.1|refseq:NP_001244259.1|refseq:NP_001244260.1|refseq:NP_001244257.1|refseq:NP_001244258.1|dip:DIP-40089N	go:"GO:0005886"(plasma membrane)	-	-	figure legend:Figure 3 C/D|comment:"In wild type APJ expressing cells, treatment with AP-13 caused a quick beta-arrestin translocation from cytoplasm to plasma membrane. The APJ/beta-arrestin complexes disappeared from the plasma membrane and were internalized into the cytosol  to form large intracellular vesicles at 60 minutes (Fig. 3C). However, AP-13 stimulated  beta-arrestin recruitment and receptor internalization were not detected in APJ I109A  mutant expressing cells even after agonist treatment for 60 minutes (Fig. 3D)"|agonist:AP-13 peptide|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-u2-os)|taxid:9606(Human U2-OS osteosarcoma cell line)	-	2019/03/29	2019/04/08	rogid:OaKR4qnsU9jANK+HB2qQ4TlanpQ9606	rogid:MY34flMH9cdhPtMLGYOQtoQyr2U9606	rigid:GAu6MgEzlELEpQSMgUf30f41CW0	false	ha tag:?-?|mutation with no effect:109-109	green fluorescent protein tag:c-c	-	-	psi-mi:"MI:1200"(immunocytochemistry)	psi-mi:"MI:0867"(tag visualisation by fluorescence)