#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:P21554 uniprotkb:P21554 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:"MI:0809"(bimolecular fluorescence complementation) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-21300159|imex:IM-26955-21 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N - - - figure legend:Supplementary Figure 1|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/04/10 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rigid:Ii9wKSEbRKFJemJQqMs0hWPAd78 false venus n-terminal fragment:c-c|ha tag:n-n venus c-terminal fragment:c-c|ha tag:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P21554 uniprotkb:P21554 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:"MI:0012"(bioluminescence resonance energy transfer) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-21300073|imex:IM-26955-1 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:2335"(luminescence acceptor) psi-mi:"MI:2334"(luminescence donor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N - - - figure legend:Figure 1-1|comment:BRET results show that CB1and D2L receptors form both homo and heteromers, with no preference to form either.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rigid:Ii9wKSEbRKFJemJQqMs0hWPAd78 false green fluorescent protein tag:c-c renilla luciferase protein tag:c-c 1 1 psi-mi:"MI:0866"(tag visualisation) psi-mi:"MI:0866"(tag visualisation) uniprotkb:P14416 uniprotkb:P14416 intact:EBI-2928178|ensembl:ENSP00000354859|ensembl:ENSP00000442172|uniprotkb:Q9NZR3|uniprotkb:Q9UPA9 intact:EBI-2928178|ensembl:ENSP00000354859|ensembl:ENSP00000442172|uniprotkb:Q9NZR3|uniprotkb:Q9UPA9 psi-mi:drd2_human(display_long)|uniprotkb:DRD2(gene name)|psi-mi:DRD2(display_short)|uniprotkb:Dopamine D2 receptor(gene name synonym) psi-mi:drd2_human(display_long)|uniprotkb:DRD2(gene name)|psi-mi:DRD2(display_short)|uniprotkb:Dopamine D2 receptor(gene name synonym) psi-mi:"MI:0012"(bioluminescence resonance energy transfer) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-21300089|imex:IM-26955-3 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:2335"(luminescence acceptor) psi-mi:"MI:2334"(luminescence donor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSG00000149295(gene)|ensembl:ENST00000362072(transcript)|ensembl:ENST00000542968(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001591"(dopamine neurotransmitter receptor activity, coupled via Gi/Go)|go:"GO:0001659"(temperature homeostasis)|go:"GO:0001666"(response to hypoxia)|go:"GO:0001669"(acrosomal vesicle)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0001975"(response to amphetamine)|go:"GO:0001976"(nervous system process involved in regulation of systemic arterial blood pressure)|go:"GO:0002027"(regulation of heart rate)|go:"GO:0002028"(regulation of sodium ion transport)|go:"GO:0002031"(G protein-coupled receptor internalization)|go:"GO:0002052"(positive regulation of neuroblast proliferation)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005929"(cilium)|go:"GO:0006874"(cellular calcium ion homeostasis)|go:"GO:0006914"(autophagy)|go:"GO:0007195"(adenylate cyclase-inhibiting dopamine receptor signaling pathway)|go:"GO:0007270"(neuron-neuron synaptic transmission)|go:"GO:0007409"(axonogenesis)|go:"GO:0007416"(synapse assembly)|go:"GO:0007608"(sensory perception of smell)|go:"GO:0007616"(long-term memory)|go:"GO:0007625"(grooming behavior)|go:"GO:0007626"(locomotory behavior)|go:"GO:0007628"(adult walking behavior)|go:"GO:0008104"(protein localization)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0008306"(associative learning)|go:"GO:0008542"(visual learning)|go:"GO:0009416"(response to light stimulus)|go:"GO:0009636"(response to toxic substance)|go:"GO:0010039"(response to iron ion)|go:"GO:0014059"(regulation of dopamine secretion)|go:"GO:0014069"(postsynaptic density)|go:"GO:0014854"(response to inactivity)|go:"GO:0015459"(potassium channel regulator activity)|go:"GO:0016055"(Wnt signaling pathway)|go:"GO:0016328"(lateral plasma membrane)|go:"GO:0021756"(striatum development)|go:"GO:0021769"(orbitofrontal cortex development)|go:"GO:0021853"(cerebral cortex GABAergic interneuron migration)|go:"GO:0021984"(adenohypophysis development)|go:"GO:0030139"(endocytic vesicle)|go:"GO:0030336"(negative regulation of cell migration)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0030432"(peristalsis)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0031223"(auditory behavior)|go:"GO:0032147"(activation of protein kinase activity)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032467"(positive regulation of cytokinesis)|go:"GO:0007194"(negative regulation of adenylate cyclase activity)|go:"GO:0032922"(circadian regulation of gene expression)|go:"GO:0034776"(response to histamine)|go:"GO:0035094"(response to nicotine)|go:"GO:0035240"(dopamine binding)|go:"GO:0035255"(ionotropic glutamate receptor binding)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0035810"(positive regulation of urine volume)|go:"GO:0035815"(positive regulation of renal sodium excretion)|go:"GO:0036126"(sperm flagellum)|go:"GO:0040018"(positive regulation of multicellular organism growth)|go:"GO:0042220"(response to cocaine)|go:"GO:0042321"(negative regulation of circadian sleep/wake cycle, sleep)|go:"GO:0042417"(dopamine metabolic process)|go:"GO:0042493"|go:"GO:0042756"(drinking behavior)|go:"GO:0042802"(identical protein binding)|go:"GO:0043197"(dendritic spine)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0043204"(perikaryon)|go:"GO:0043266"(regulation of potassium ion transport)|go:"GO:0043278"(response to morphine)|go:"GO:0043473"(pigmentation)|go:"GO:0043666"(regulation of phosphoprotein phosphatase activity)|go:"GO:0043679"(axon terminus)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045202"(synapse)|go:"GO:0045745"(positive regulation of G protein-coupled receptor signaling pathway)|go:"GO:0045824"(negative regulation of innate immune response)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046676"(negative regulation of insulin secretion)|go:"GO:0046717"(acid secretion)|go:"GO:0048148"(behavioral response to cocaine)|go:"GO:0048149"(behavioral response to ethanol)|go:"GO:0048169"(regulation of long-term neuronal synaptic plasticity)|go:"GO:0048678"(response to axon injury)|go:"GO:0048755"(branching morphogenesis of a nerve)|go:"GO:0050482"(arachidonic acid secretion)|go:"GO:0050709"(negative regulation of protein secretion)|go:"GO:0051209"(release of sequestered calcium ion into cytosol)|go:"GO:0051481"(negative regulation of cytosolic calcium ion concentration)|go:"GO:0051482"(positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway)|go:"GO:0051584"(regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0051586"(positive regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0051898"(negative regulation of protein kinase B signaling)|go:"GO:0051967"(negative regulation of synaptic transmission, glutamatergic)|go:"GO:0060079"(excitatory postsynaptic potential)|go:"GO:0060124"(positive regulation of growth hormone secretion)|go:"GO:0060134"(prepulse inhibition)|go:"GO:0060158"(phospholipase C-activating dopamine receptor signaling pathway)|go:"GO:0060160"(negative regulation of dopamine receptor signaling pathway)|go:"GO:0060170"(ciliary membrane)|go:"GO:0060548"(negative regulation of cell death)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071880"(adenylate cyclase-activating adrenergic receptor signaling pathway)|go:"GO:0090325"(regulation of locomotion involved in locomotory behavior)|go:"GO:0097730"(non-motile cilium)|go:"GO:0098691"(dopaminergic synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099055"(integral component of postsynaptic membrane)|go:"GO:0099056"(integral component of presynaptic membrane)|go:"GO:0099170"(postsynaptic modulation of chemical synaptic transmission)|go:"GO:1900168"(positive regulation of glial cell-derived neurotrophic factor production)|go:"GO:1900273"(positive regulation of long-term synaptic potentiation)|go:"GO:1901386"(negative regulation of voltage-gated calcium channel activity)|go:"GO:1990384"(hyaloid vascular plexus regression)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000929(Dopamine receptor)|interpro:IPR001922(Dopamine D2 receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|mint:P14416|rcsb pdb:1I15|rcsb pdb:5AER|rcsb pdb:6CM4|rcsb pdb:6LUQ|rcsb pdb:6VMS|rcsb pdb:7JVR|reactome:R-HSA-390651|go:"GO:0051823"(regulation of synapse structural plasticity)|refseq:NP_000786.1|refseq:NP_057658.2|refseq:XP_016872785.1|dip:DIP-5977N ensembl:ENSG00000149295(gene)|ensembl:ENST00000362072(transcript)|ensembl:ENST00000542968(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001591"(dopamine neurotransmitter receptor activity, coupled via Gi/Go)|go:"GO:0001659"(temperature homeostasis)|go:"GO:0001666"(response to hypoxia)|go:"GO:0001669"(acrosomal vesicle)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0001975"(response to amphetamine)|go:"GO:0001976"(nervous system process involved in regulation of systemic arterial blood pressure)|go:"GO:0002027"(regulation of heart rate)|go:"GO:0002028"(regulation of sodium ion transport)|go:"GO:0002031"(G protein-coupled receptor internalization)|go:"GO:0002052"(positive regulation of neuroblast proliferation)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005929"(cilium)|go:"GO:0006874"(cellular calcium ion homeostasis)|go:"GO:0006914"(autophagy)|go:"GO:0007195"(adenylate cyclase-inhibiting dopamine receptor signaling pathway)|go:"GO:0007270"(neuron-neuron synaptic transmission)|go:"GO:0007409"(axonogenesis)|go:"GO:0007416"(synapse assembly)|go:"GO:0007608"(sensory perception of smell)|go:"GO:0007616"(long-term memory)|go:"GO:0007625"(grooming behavior)|go:"GO:0007626"(locomotory behavior)|go:"GO:0007628"(adult walking behavior)|go:"GO:0008104"(protein localization)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0008306"(associative learning)|go:"GO:0008542"(visual learning)|go:"GO:0009416"(response to light stimulus)|go:"GO:0009636"(response to toxic substance)|go:"GO:0010039"(response to iron ion)|go:"GO:0014059"(regulation of dopamine secretion)|go:"GO:0014069"(postsynaptic density)|go:"GO:0014854"(response to inactivity)|go:"GO:0015459"(potassium channel regulator activity)|go:"GO:0016055"(Wnt signaling pathway)|go:"GO:0016328"(lateral plasma membrane)|go:"GO:0021756"(striatum development)|go:"GO:0021769"(orbitofrontal cortex development)|go:"GO:0021853"(cerebral cortex GABAergic interneuron migration)|go:"GO:0021984"(adenohypophysis development)|go:"GO:0030139"(endocytic vesicle)|go:"GO:0030336"(negative regulation of cell migration)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0030432"(peristalsis)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0031223"(auditory behavior)|go:"GO:0032147"(activation of protein kinase activity)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032467"(positive regulation of cytokinesis)|go:"GO:0007194"(negative regulation of adenylate cyclase activity)|go:"GO:0032922"(circadian regulation of gene expression)|go:"GO:0034776"(response to histamine)|go:"GO:0035094"(response to nicotine)|go:"GO:0035240"(dopamine binding)|go:"GO:0035255"(ionotropic glutamate receptor binding)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0035810"(positive regulation of urine volume)|go:"GO:0035815"(positive regulation of renal sodium excretion)|go:"GO:0036126"(sperm flagellum)|go:"GO:0040018"(positive regulation of multicellular organism growth)|go:"GO:0042220"(response to cocaine)|go:"GO:0042321"(negative regulation of circadian sleep/wake cycle, sleep)|go:"GO:0042417"(dopamine metabolic process)|go:"GO:0042493"|go:"GO:0042756"(drinking behavior)|go:"GO:0042802"(identical protein binding)|go:"GO:0043197"(dendritic spine)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0043204"(perikaryon)|go:"GO:0043266"(regulation of potassium ion transport)|go:"GO:0043278"(response to morphine)|go:"GO:0043473"(pigmentation)|go:"GO:0043666"(regulation of phosphoprotein phosphatase activity)|go:"GO:0043679"(axon terminus)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045202"(synapse)|go:"GO:0045745"(positive regulation of G protein-coupled receptor signaling pathway)|go:"GO:0045824"(negative regulation of innate immune response)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046676"(negative regulation of insulin secretion)|go:"GO:0046717"(acid secretion)|go:"GO:0048148"(behavioral response to cocaine)|go:"GO:0048149"(behavioral response to ethanol)|go:"GO:0048169"(regulation of long-term neuronal synaptic plasticity)|go:"GO:0048678"(response to axon injury)|go:"GO:0048755"(branching morphogenesis of a nerve)|go:"GO:0050482"(arachidonic acid secretion)|go:"GO:0050709"(negative regulation of protein secretion)|go:"GO:0051209"(release of sequestered calcium ion into cytosol)|go:"GO:0051481"(negative regulation of cytosolic calcium ion concentration)|go:"GO:0051482"(positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway)|go:"GO:0051584"(regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0051586"(positive regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0051898"(negative regulation of protein kinase B signaling)|go:"GO:0051967"(negative regulation of synaptic transmission, glutamatergic)|go:"GO:0060079"(excitatory postsynaptic potential)|go:"GO:0060124"(positive regulation of growth hormone secretion)|go:"GO:0060134"(prepulse inhibition)|go:"GO:0060158"(phospholipase C-activating dopamine receptor signaling pathway)|go:"GO:0060160"(negative regulation of dopamine receptor signaling pathway)|go:"GO:0060170"(ciliary membrane)|go:"GO:0060548"(negative regulation of cell death)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071880"(adenylate cyclase-activating adrenergic receptor signaling pathway)|go:"GO:0090325"(regulation of locomotion involved in locomotory behavior)|go:"GO:0097730"(non-motile cilium)|go:"GO:0098691"(dopaminergic synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099055"(integral component of postsynaptic membrane)|go:"GO:0099056"(integral component of presynaptic membrane)|go:"GO:0099170"(postsynaptic modulation of chemical synaptic transmission)|go:"GO:1900168"(positive regulation of glial cell-derived neurotrophic factor production)|go:"GO:1900273"(positive regulation of long-term synaptic potentiation)|go:"GO:1901386"(negative regulation of voltage-gated calcium channel activity)|go:"GO:1990384"(hyaloid vascular plexus regression)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000929(Dopamine receptor)|interpro:IPR001922(Dopamine D2 receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|mint:P14416|rcsb pdb:1I15|rcsb pdb:5AER|rcsb pdb:6CM4|rcsb pdb:6LUQ|rcsb pdb:6VMS|rcsb pdb:7JVR|reactome:R-HSA-390651|go:"GO:0051823"(regulation of synapse structural plasticity)|refseq:NP_000786.1|refseq:NP_057658.2|refseq:XP_016872785.1|dip:DIP-5977N - - - figure legend:Figure 1-2|comment:BRET results show that CB1and D2L receptors form both homo and heteromers, with no preference to form either.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:S5beCpwOapTeUXcS45WlU5ApKEI9606 rogid:S5beCpwOapTeUXcS45WlU5ApKEI9606 rigid:CRVkrHeAvCUc+jiNkNp4h0DnrsI false green fluorescent protein tag:c-c renilla luciferase protein tag:c-c 1 1 psi-mi:"MI:0866"(tag visualisation) psi-mi:"MI:0866"(tag visualisation) uniprotkb:P21554 uniprotkb:P21554 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:"MI:0012"(bioluminescence resonance energy transfer) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-21300108|imex:IM-26955-5 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:2334"(luminescence donor) psi-mi:"MI:2334"(luminescence donor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N - - - figure legend:Figure 1-3|comment:BRET results show that CB1and D2L receptors form both homo and heteromers, with no preference to form either.|comment:"Co-expression of CB1 and D2L resulted in a calculated oligomerization state value of N= 2.61 ± 0.24 (Fig. 1B), which implied that CB 1 /D 2L heteromers were present as tetramers or higher-order oligomeric complexes."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rigid:BgN+WYynSK4bN6LGSe2Vb6xqtw4 false renilla luciferase protein tag:c-c renilla luciferase protein tag:c-c - - psi-mi:"MI:0866"(tag visualisation) psi-mi:"MI:0866"(tag visualisation) uniprotkb:P21554 uniprotkb:P14416 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 intact:EBI-2928178|ensembl:ENSP00000354859|ensembl:ENSP00000442172|uniprotkb:Q9NZR3|uniprotkb:Q9UPA9 psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:drd2_human(display_long)|uniprotkb:DRD2(gene name)|psi-mi:DRD2(display_short)|uniprotkb:Dopamine D2 receptor(gene name synonym) psi-mi:"MI:0012"(bioluminescence resonance energy transfer) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-21300108|imex:IM-26955-5 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:2334"(luminescence donor) psi-mi:"MI:2335"(luminescence acceptor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N ensembl:ENSG00000149295(gene)|ensembl:ENST00000362072(transcript)|ensembl:ENST00000542968(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001591"(dopamine neurotransmitter receptor activity, coupled via Gi/Go)|go:"GO:0001659"(temperature homeostasis)|go:"GO:0001666"(response to hypoxia)|go:"GO:0001669"(acrosomal vesicle)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0001975"(response to amphetamine)|go:"GO:0001976"(nervous system process involved in regulation of systemic arterial blood pressure)|go:"GO:0002027"(regulation of heart rate)|go:"GO:0002028"(regulation of sodium ion transport)|go:"GO:0002031"(G protein-coupled receptor internalization)|go:"GO:0002052"(positive regulation of neuroblast proliferation)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005929"(cilium)|go:"GO:0006874"(cellular calcium ion homeostasis)|go:"GO:0006914"(autophagy)|go:"GO:0007195"(adenylate cyclase-inhibiting dopamine receptor signaling pathway)|go:"GO:0007270"(neuron-neuron synaptic transmission)|go:"GO:0007409"(axonogenesis)|go:"GO:0007416"(synapse assembly)|go:"GO:0007608"(sensory perception of smell)|go:"GO:0007616"(long-term memory)|go:"GO:0007625"(grooming behavior)|go:"GO:0007626"(locomotory behavior)|go:"GO:0007628"(adult walking behavior)|go:"GO:0008104"(protein localization)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0008306"(associative learning)|go:"GO:0008542"(visual learning)|go:"GO:0009416"(response to light stimulus)|go:"GO:0009636"(response to toxic substance)|go:"GO:0010039"(response to iron ion)|go:"GO:0014059"(regulation of dopamine secretion)|go:"GO:0014069"(postsynaptic density)|go:"GO:0014854"(response to inactivity)|go:"GO:0015459"(potassium channel regulator activity)|go:"GO:0016055"(Wnt signaling pathway)|go:"GO:0016328"(lateral plasma membrane)|go:"GO:0021756"(striatum development)|go:"GO:0021769"(orbitofrontal cortex development)|go:"GO:0021853"(cerebral cortex GABAergic interneuron migration)|go:"GO:0021984"(adenohypophysis development)|go:"GO:0030139"(endocytic vesicle)|go:"GO:0030336"(negative regulation of cell migration)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0030432"(peristalsis)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0031223"(auditory behavior)|go:"GO:0032147"(activation of protein kinase activity)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032467"(positive regulation of cytokinesis)|go:"GO:0007194"(negative regulation of adenylate cyclase activity)|go:"GO:0032922"(circadian regulation of gene expression)|go:"GO:0034776"(response to histamine)|go:"GO:0035094"(response to nicotine)|go:"GO:0035240"(dopamine binding)|go:"GO:0035255"(ionotropic glutamate receptor binding)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0035810"(positive regulation of urine volume)|go:"GO:0035815"(positive regulation of renal sodium excretion)|go:"GO:0036126"(sperm flagellum)|go:"GO:0040018"(positive regulation of multicellular organism growth)|go:"GO:0042220"(response to cocaine)|go:"GO:0042321"(negative regulation of circadian sleep/wake cycle, sleep)|go:"GO:0042417"(dopamine metabolic process)|go:"GO:0042493"|go:"GO:0042756"(drinking behavior)|go:"GO:0042802"(identical protein binding)|go:"GO:0043197"(dendritic spine)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0043204"(perikaryon)|go:"GO:0043266"(regulation of potassium ion transport)|go:"GO:0043278"(response to morphine)|go:"GO:0043473"(pigmentation)|go:"GO:0043666"(regulation of phosphoprotein phosphatase activity)|go:"GO:0043679"(axon terminus)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045202"(synapse)|go:"GO:0045745"(positive regulation of G protein-coupled receptor signaling pathway)|go:"GO:0045824"(negative regulation of innate immune response)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046676"(negative regulation of insulin secretion)|go:"GO:0046717"(acid secretion)|go:"GO:0048148"(behavioral response to cocaine)|go:"GO:0048149"(behavioral response to ethanol)|go:"GO:0048169"(regulation of long-term neuronal synaptic plasticity)|go:"GO:0048678"(response to axon injury)|go:"GO:0048755"(branching morphogenesis of a nerve)|go:"GO:0050482"(arachidonic acid secretion)|go:"GO:0050709"(negative regulation of protein secretion)|go:"GO:0051209"(release of sequestered calcium ion into cytosol)|go:"GO:0051481"(negative regulation of cytosolic calcium ion concentration)|go:"GO:0051482"(positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway)|go:"GO:0051584"(regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0051586"(positive regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0051898"(negative regulation of protein kinase B signaling)|go:"GO:0051967"(negative regulation of synaptic transmission, glutamatergic)|go:"GO:0060079"(excitatory postsynaptic potential)|go:"GO:0060124"(positive regulation of growth hormone secretion)|go:"GO:0060134"(prepulse inhibition)|go:"GO:0060158"(phospholipase C-activating dopamine receptor signaling pathway)|go:"GO:0060160"(negative regulation of dopamine receptor signaling pathway)|go:"GO:0060170"(ciliary membrane)|go:"GO:0060548"(negative regulation of cell death)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071880"(adenylate cyclase-activating adrenergic receptor signaling pathway)|go:"GO:0090325"(regulation of locomotion involved in locomotory behavior)|go:"GO:0097730"(non-motile cilium)|go:"GO:0098691"(dopaminergic synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099055"(integral component of postsynaptic membrane)|go:"GO:0099056"(integral component of presynaptic membrane)|go:"GO:0099170"(postsynaptic modulation of chemical synaptic transmission)|go:"GO:1900168"(positive regulation of glial cell-derived neurotrophic factor production)|go:"GO:1900273"(positive regulation of long-term synaptic potentiation)|go:"GO:1901386"(negative regulation of voltage-gated calcium channel activity)|go:"GO:1990384"(hyaloid vascular plexus regression)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000929(Dopamine receptor)|interpro:IPR001922(Dopamine D2 receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|mint:P14416|rcsb pdb:1I15|rcsb pdb:5AER|rcsb pdb:6CM4|rcsb pdb:6LUQ|rcsb pdb:6VMS|rcsb pdb:7JVR|reactome:R-HSA-390651|go:"GO:0051823"(regulation of synapse structural plasticity)|refseq:NP_000786.1|refseq:NP_057658.2|refseq:XP_016872785.1|dip:DIP-5977N - - - figure legend:Figure 1-3|comment:BRET results show that CB1and D2L receptors form both homo and heteromers, with no preference to form either.|comment:"Co-expression of CB1 and D2L resulted in a calculated oligomerization state value of N= 2.61 ± 0.24 (Fig. 1B), which implied that CB 1 /D 2L heteromers were present as tetramers or higher-order oligomeric complexes."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rogid:S5beCpwOapTeUXcS45WlU5ApKEI9606 rigid:BgN+WYynSK4bN6LGSe2Vb6xqtw4 false renilla luciferase protein tag:c-c green fluorescent protein tag:c-c - - psi-mi:"MI:0866"(tag visualisation) psi-mi:"MI:0866"(tag visualisation) uniprotkb:P21554 uniprotkb:P14416 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 intact:EBI-2928178|ensembl:ENSP00000354859|ensembl:ENSP00000442172|uniprotkb:Q9NZR3|uniprotkb:Q9UPA9 psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:drd2_human(display_long)|uniprotkb:DRD2(gene name)|psi-mi:DRD2(display_short)|uniprotkb:Dopamine D2 receptor(gene name synonym) psi-mi:"MI:0012"(bioluminescence resonance energy transfer) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-21300108|imex:IM-26955-5 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:2334"(luminescence donor) psi-mi:"MI:2335"(luminescence acceptor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N ensembl:ENSG00000149295(gene)|ensembl:ENST00000362072(transcript)|ensembl:ENST00000542968(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001591"(dopamine neurotransmitter receptor activity, coupled via Gi/Go)|go:"GO:0001659"(temperature homeostasis)|go:"GO:0001666"(response to hypoxia)|go:"GO:0001669"(acrosomal vesicle)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0001975"(response to amphetamine)|go:"GO:0001976"(nervous system process involved in regulation of systemic arterial blood pressure)|go:"GO:0002027"(regulation of heart rate)|go:"GO:0002028"(regulation of sodium ion transport)|go:"GO:0002031"(G protein-coupled receptor internalization)|go:"GO:0002052"(positive regulation of neuroblast proliferation)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005929"(cilium)|go:"GO:0006874"(cellular calcium ion homeostasis)|go:"GO:0006914"(autophagy)|go:"GO:0007195"(adenylate cyclase-inhibiting dopamine receptor signaling pathway)|go:"GO:0007270"(neuron-neuron synaptic transmission)|go:"GO:0007409"(axonogenesis)|go:"GO:0007416"(synapse assembly)|go:"GO:0007608"(sensory perception of smell)|go:"GO:0007616"(long-term memory)|go:"GO:0007625"(grooming behavior)|go:"GO:0007626"(locomotory behavior)|go:"GO:0007628"(adult walking behavior)|go:"GO:0008104"(protein localization)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0008306"(associative learning)|go:"GO:0008542"(visual learning)|go:"GO:0009416"(response to light stimulus)|go:"GO:0009636"(response to toxic substance)|go:"GO:0010039"(response to iron ion)|go:"GO:0014059"(regulation of dopamine secretion)|go:"GO:0014069"(postsynaptic density)|go:"GO:0014854"(response to inactivity)|go:"GO:0015459"(potassium channel regulator activity)|go:"GO:0016055"(Wnt signaling pathway)|go:"GO:0016328"(lateral plasma membrane)|go:"GO:0021756"(striatum development)|go:"GO:0021769"(orbitofrontal cortex development)|go:"GO:0021853"(cerebral cortex GABAergic interneuron migration)|go:"GO:0021984"(adenohypophysis development)|go:"GO:0030139"(endocytic vesicle)|go:"GO:0030336"(negative regulation of cell migration)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0030432"(peristalsis)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0031223"(auditory behavior)|go:"GO:0032147"(activation of protein kinase activity)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032467"(positive regulation of cytokinesis)|go:"GO:0007194"(negative regulation of adenylate cyclase activity)|go:"GO:0032922"(circadian regulation of gene expression)|go:"GO:0034776"(response to histamine)|go:"GO:0035094"(response to nicotine)|go:"GO:0035240"(dopamine binding)|go:"GO:0035255"(ionotropic glutamate receptor binding)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0035810"(positive regulation of urine volume)|go:"GO:0035815"(positive regulation of renal sodium excretion)|go:"GO:0036126"(sperm flagellum)|go:"GO:0040018"(positive regulation of multicellular organism growth)|go:"GO:0042220"(response to cocaine)|go:"GO:0042321"(negative regulation of circadian sleep/wake cycle, sleep)|go:"GO:0042417"(dopamine metabolic process)|go:"GO:0042493"|go:"GO:0042756"(drinking behavior)|go:"GO:0042802"(identical protein binding)|go:"GO:0043197"(dendritic spine)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0043204"(perikaryon)|go:"GO:0043266"(regulation of potassium ion transport)|go:"GO:0043278"(response to morphine)|go:"GO:0043473"(pigmentation)|go:"GO:0043666"(regulation of phosphoprotein phosphatase activity)|go:"GO:0043679"(axon terminus)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045202"(synapse)|go:"GO:0045745"(positive regulation of G protein-coupled receptor signaling pathway)|go:"GO:0045824"(negative regulation of innate immune response)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046676"(negative regulation of insulin secretion)|go:"GO:0046717"(acid secretion)|go:"GO:0048148"(behavioral response to cocaine)|go:"GO:0048149"(behavioral response to ethanol)|go:"GO:0048169"(regulation of long-term neuronal synaptic plasticity)|go:"GO:0048678"(response to axon injury)|go:"GO:0048755"(branching morphogenesis of a nerve)|go:"GO:0050482"(arachidonic acid secretion)|go:"GO:0050709"(negative regulation of protein secretion)|go:"GO:0051209"(release of sequestered calcium ion into cytosol)|go:"GO:0051481"(negative regulation of cytosolic calcium ion concentration)|go:"GO:0051482"(positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway)|go:"GO:0051584"(regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0051586"(positive regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0051898"(negative regulation of protein kinase B signaling)|go:"GO:0051967"(negative regulation of synaptic transmission, glutamatergic)|go:"GO:0060079"(excitatory postsynaptic potential)|go:"GO:0060124"(positive regulation of growth hormone secretion)|go:"GO:0060134"(prepulse inhibition)|go:"GO:0060158"(phospholipase C-activating dopamine receptor signaling pathway)|go:"GO:0060160"(negative regulation of dopamine receptor signaling pathway)|go:"GO:0060170"(ciliary membrane)|go:"GO:0060548"(negative regulation of cell death)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071880"(adenylate cyclase-activating adrenergic receptor signaling pathway)|go:"GO:0090325"(regulation of locomotion involved in locomotory behavior)|go:"GO:0097730"(non-motile cilium)|go:"GO:0098691"(dopaminergic synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099055"(integral component of postsynaptic membrane)|go:"GO:0099056"(integral component of presynaptic membrane)|go:"GO:0099170"(postsynaptic modulation of chemical synaptic transmission)|go:"GO:1900168"(positive regulation of glial cell-derived neurotrophic factor production)|go:"GO:1900273"(positive regulation of long-term synaptic potentiation)|go:"GO:1901386"(negative regulation of voltage-gated calcium channel activity)|go:"GO:1990384"(hyaloid vascular plexus regression)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000929(Dopamine receptor)|interpro:IPR001922(Dopamine D2 receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|mint:P14416|rcsb pdb:1I15|rcsb pdb:5AER|rcsb pdb:6CM4|rcsb pdb:6LUQ|rcsb pdb:6VMS|rcsb pdb:7JVR|reactome:R-HSA-390651|go:"GO:0051823"(regulation of synapse structural plasticity)|refseq:NP_000786.1|refseq:NP_057658.2|refseq:XP_016872785.1|dip:DIP-5977N - - - figure legend:Figure 1-3|comment:BRET results show that CB1and D2L receptors form both homo and heteromers, with no preference to form either.|comment:"Co-expression of CB1 and D2L resulted in a calculated oligomerization state value of N= 2.61 ± 0.24 (Fig. 1B), which implied that CB 1 /D 2L heteromers were present as tetramers or higher-order oligomeric complexes."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rogid:S5beCpwOapTeUXcS45WlU5ApKEI9606 rigid:BgN+WYynSK4bN6LGSe2Vb6xqtw4 false renilla luciferase protein tag:c-c green fluorescent protein tag:c-c - - psi-mi:"MI:0866"(tag visualisation) psi-mi:"MI:0866"(tag visualisation) uniprotkb:P63096 uniprotkb:P21554 intact:EBI-618639|ensembl:ENSP00000497051|ensembl:ENSP00000497179|ensembl:ENSP00000497260|ensembl:ENSP00000497315|ensembl:ENSP00000497379|ensembl:ENSP00000497717|ensembl:ENSP00000497754|ensembl:ENSP00000497760|ensembl:ENSP00000497765|ensembl:ENSP00000497773|ensembl:ENSP00000498091|ensembl:ENSP00000343027|ensembl:ENSP00000391439|ensembl:ENSP00000496800|ensembl:ENSP00000496829|uniprotkb:P04898|uniprotkb:P11015|uniprotkb:P31871|uniprotkb:Q5U074|uniprotkb:Q8TAN5|uniprotkb:Q9UGA4|uniprotkb:A8KA88|uniprotkb:B4E2V1|uniprotkb:C9J3A4 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 psi-mi:gnai1_human(display_long)|uniprotkb:GNAI1(gene name)|psi-mi:GNAI1(display_short)|uniprotkb:Adenylate cyclase-inhibiting G alpha protein(gene name synonym) psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:"MI:0012"(bioluminescence resonance energy transfer) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-21300397|imex:IM-26955-36 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:2334"(luminescence donor) psi-mi:"MI:2335"(luminescence acceptor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) reactome:R-HSA-418597|reactome:R-HSA-422356|reactome:R-HSA-9009391|reactome:R-HSA-9660821|reactome:R-HSA-418594|ensembl:ENST00000351004(transcript)|ensembl:ENST00000442586(transcript)|ensembl:ENST00000648098(transcript)|ensembl:ENSG00000127955(gene)|ensembl:ENST00000648306(transcript)|ensembl:ENST00000648412(transcript)|ensembl:ENST00000648476(transcript)|ensembl:ENST00000648663(transcript)|ensembl:ENST00000648832(transcript)|ensembl:ENST00000648877(transcript)|ensembl:ENST00000648953(transcript)|ensembl:ENST00000649225(transcript)|ensembl:ENST00000649267(transcript)|ensembl:ENST00000649487(transcript)|ensembl:ENST00000649796(transcript)|ensembl:ENST00000649855(transcript)|go:"GO:0000287"(magnesium ion binding)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005765"(lysosomal membrane)|go:"GO:0005813"(centrosome)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0007049"(cell cycle)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007193"(adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway)|go:"GO:0019003"(GDP binding)|go:"GO:0030496"(midbody)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0031821"(G protein-coupled serotonin receptor binding)|go:"GO:0032794"(GTPase activating protein binding)|go:"GO:0043434"(response to peptide hormone)|go:"GO:0043949"(regulation of cAMP-mediated signaling)|go:"GO:0045121"(membrane raft)|go:"GO:0050805"(negative regulation of synaptic transmission)|go:"GO:0051301"(cell division)|go:"GO:0060236"(regulation of mitotic spindle organization)|go:"GO:0070062"(extracellular exosome)|go:"GO:0099738"(cell cortex region)|go:"GO:1904322"(cellular response to forskolin)|go:"GO:1904778"(positive regulation of protein localization to cell cortex)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR001408(G-protein alpha subunit, group I)|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|mint:P63096|rcsb pdb:1KJY|rcsb pdb:1Y3A|rcsb pdb:2G83|rcsb pdb:2GTP|rcsb pdb:2IK8|rcsb pdb:2OM2|rcsb pdb:2XNS|rcsb pdb:3ONW|rcsb pdb:3QE0|rcsb pdb:3QI2|rcsb pdb:3UMR|rcsb pdb:3UMS|rcsb pdb:4G5Q|rcsb pdb:5JS7|rcsb pdb:5JS8|rcsb pdb:5TDH|rcsb pdb:6CMO|rcsb pdb:6CRK|rcsb pdb:6DDE|rcsb pdb:6DDF|rcsb pdb:6KPF|rcsb pdb:6KPG|rcsb pdb:6LFM|rcsb pdb:6LFO|rcsb pdb:6LML|rcsb pdb:6N4B|rcsb pdb:6OMM|rcsb pdb:6OS9|rcsb pdb:6OSA|rcsb pdb:6OT0|rcsb pdb:6PB0|rcsb pdb:6PB1|rcsb pdb:6PT0|rcsb pdb:6QNO|rcsb pdb:6VU8|rcsb pdb:6XBJ|rcsb pdb:6XBK|rcsb pdb:6XBL|rcsb pdb:6XBM|rcsb pdb:7CMU|rcsb pdb:7CMV|rcsb pdb:7E2X|rcsb pdb:7E2Y|rcsb pdb:7E2Z|rcsb pdb:7E32|rcsb pdb:7E33|rcsb pdb:7E9G|rcsb pdb:7EB2|rcsb pdb:7JVR|rcsb pdb:7L0P|rcsb pdb:7L0Q|rcsb pdb:7L0R|rcsb pdb:7L0S|rcsb pdb:7O7F|reactome:R-HSA-170670|reactome:R-HSA-392170|reactome:R-HSA-400042|reactome:R-HSA-418555|refseq:NP_002060.4|refseq:NP_001243343.1 go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N - - - figure legend:Figure 3A|agonist:"Arachidonyl-2'-chloroethylamide (ACEA) - a selective, synthetic agonist of CB1R."|comment:Untagged D2L was co-expressed.|antagonist:"Quinpirole (D2L agonist) added concurrently with Arachidonyl-2'-chloroethylamide (ACEA) - a selective, synthetic agonist of CB1R."|comment:Treating the cells with 1 uM ACEA and increasing the concentration of quinpirole resulted in concentration-dependent inhibition in BRET Eff between Gai-Rluc and CB1-GFP 2.|comment:Co-expression and co-activation of either D2L and beta2 did not alter CB1 coupling to G proteins.|agonist:"Quinpirole (D2L agonist) but only in the presence of untagged D2L. This increase in BRET Eff was PTx-sensitive."|comment:"PTx (Pertussis toxin) blocked ACEA-dependent and also Quinpirole+D2L dependent increases in BRET Efficiency."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:3yQOCXJb8Undp/Xw42xrBiG5ePE9606 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rigid:IzLXYnd5auXbNl36x+xrzzb18Bw false renilla luciferase protein tag:93-94 green fluorescent protein tag:c-c - - psi-mi:"MI:0866"(tag visualisation) psi-mi:"MI:0866"(tag visualisation) uniprotkb:P21554 uniprotkb:P21554 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:"MI:0012"(bioluminescence resonance energy transfer) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-21300409|imex:IM-26955-38 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0941"(competitor) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:2335"(luminescence acceptor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N - - - figure legend:Figure 4B-2|agonist:"Arachidonyl-2'-chloroethylamide (ACEA) - a selective, synthetic agonist of CB1R. In the presence of the blocking peptide agonist activity was reduced."|comment:Untagged D2L was co-expressed.|agonist:"Quinpirole (D2L agonist). The presence of the blocking peptide did not significantly affect the action of Quinpirole."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rigid:IzLXYnd5auXbNl36x+xrzzb18Bw false sufficient binding region:300-431 green fluorescent protein tag:c-c - - psi-mi:"MI:0866"(tag visualisation) psi-mi:"MI:0866"(tag visualisation) uniprotkb:P21554 uniprotkb:P63096 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 intact:EBI-618639|ensembl:ENSP00000497051|ensembl:ENSP00000497179|ensembl:ENSP00000497260|ensembl:ENSP00000497315|ensembl:ENSP00000497379|ensembl:ENSP00000497717|ensembl:ENSP00000497754|ensembl:ENSP00000497760|ensembl:ENSP00000497765|ensembl:ENSP00000497773|ensembl:ENSP00000498091|ensembl:ENSP00000343027|ensembl:ENSP00000391439|ensembl:ENSP00000496800|ensembl:ENSP00000496829|uniprotkb:P04898|uniprotkb:P11015|uniprotkb:P31871|uniprotkb:Q5U074|uniprotkb:Q8TAN5|uniprotkb:Q9UGA4|uniprotkb:A8KA88|uniprotkb:B4E2V1|uniprotkb:C9J3A4 psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:gnai1_human(display_long)|uniprotkb:GNAI1(gene name)|psi-mi:GNAI1(display_short)|uniprotkb:Adenylate cyclase-inhibiting G alpha protein(gene name synonym) psi-mi:"MI:0012"(bioluminescence resonance energy transfer) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-21300409|imex:IM-26955-38 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0941"(competitor) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:2334"(luminescence donor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N reactome:R-HSA-418597|reactome:R-HSA-422356|reactome:R-HSA-9009391|reactome:R-HSA-9660821|reactome:R-HSA-418594|ensembl:ENST00000351004(transcript)|ensembl:ENST00000442586(transcript)|ensembl:ENST00000648098(transcript)|ensembl:ENSG00000127955(gene)|ensembl:ENST00000648306(transcript)|ensembl:ENST00000648412(transcript)|ensembl:ENST00000648476(transcript)|ensembl:ENST00000648663(transcript)|ensembl:ENST00000648832(transcript)|ensembl:ENST00000648877(transcript)|ensembl:ENST00000648953(transcript)|ensembl:ENST00000649225(transcript)|ensembl:ENST00000649267(transcript)|ensembl:ENST00000649487(transcript)|ensembl:ENST00000649796(transcript)|ensembl:ENST00000649855(transcript)|go:"GO:0000287"(magnesium ion binding)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005765"(lysosomal membrane)|go:"GO:0005813"(centrosome)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0007049"(cell cycle)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007193"(adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway)|go:"GO:0019003"(GDP binding)|go:"GO:0030496"(midbody)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0031821"(G protein-coupled serotonin receptor binding)|go:"GO:0032794"(GTPase activating protein binding)|go:"GO:0043434"(response to peptide hormone)|go:"GO:0043949"(regulation of cAMP-mediated signaling)|go:"GO:0045121"(membrane raft)|go:"GO:0050805"(negative regulation of synaptic transmission)|go:"GO:0051301"(cell division)|go:"GO:0060236"(regulation of mitotic spindle organization)|go:"GO:0070062"(extracellular exosome)|go:"GO:0099738"(cell cortex region)|go:"GO:1904322"(cellular response to forskolin)|go:"GO:1904778"(positive regulation of protein localization to cell cortex)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR001408(G-protein alpha subunit, group I)|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|mint:P63096|rcsb pdb:1KJY|rcsb pdb:1Y3A|rcsb pdb:2G83|rcsb pdb:2GTP|rcsb pdb:2IK8|rcsb pdb:2OM2|rcsb pdb:2XNS|rcsb pdb:3ONW|rcsb pdb:3QE0|rcsb pdb:3QI2|rcsb pdb:3UMR|rcsb pdb:3UMS|rcsb pdb:4G5Q|rcsb pdb:5JS7|rcsb pdb:5JS8|rcsb pdb:5TDH|rcsb pdb:6CMO|rcsb pdb:6CRK|rcsb pdb:6DDE|rcsb pdb:6DDF|rcsb pdb:6KPF|rcsb pdb:6KPG|rcsb pdb:6LFM|rcsb pdb:6LFO|rcsb pdb:6LML|rcsb pdb:6N4B|rcsb pdb:6OMM|rcsb pdb:6OS9|rcsb pdb:6OSA|rcsb pdb:6OT0|rcsb pdb:6PB0|rcsb pdb:6PB1|rcsb pdb:6PT0|rcsb pdb:6QNO|rcsb pdb:6VU8|rcsb pdb:6XBJ|rcsb pdb:6XBK|rcsb pdb:6XBL|rcsb pdb:6XBM|rcsb pdb:7CMU|rcsb pdb:7CMV|rcsb pdb:7E2X|rcsb pdb:7E2Y|rcsb pdb:7E2Z|rcsb pdb:7E32|rcsb pdb:7E33|rcsb pdb:7E9G|rcsb pdb:7EB2|rcsb pdb:7JVR|rcsb pdb:7L0P|rcsb pdb:7L0Q|rcsb pdb:7L0R|rcsb pdb:7L0S|rcsb pdb:7O7F|reactome:R-HSA-170670|reactome:R-HSA-392170|reactome:R-HSA-400042|reactome:R-HSA-418555|refseq:NP_002060.4|refseq:NP_001243343.1 - - - figure legend:Figure 4B-2|agonist:"Arachidonyl-2'-chloroethylamide (ACEA) - a selective, synthetic agonist of CB1R. In the presence of the blocking peptide agonist activity was reduced."|comment:Untagged D2L was co-expressed.|agonist:"Quinpirole (D2L agonist). The presence of the blocking peptide did not significantly affect the action of Quinpirole."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rogid:3yQOCXJb8Undp/Xw42xrBiG5ePE9606 rigid:IzLXYnd5auXbNl36x+xrzzb18Bw false sufficient binding region:300-431 renilla luciferase protein tag:93-94 - - psi-mi:"MI:0866"(tag visualisation) psi-mi:"MI:0866"(tag visualisation) uniprotkb:P63096 uniprotkb:P14416 intact:EBI-618639|ensembl:ENSP00000497051|ensembl:ENSP00000497179|ensembl:ENSP00000497260|ensembl:ENSP00000497315|ensembl:ENSP00000497379|ensembl:ENSP00000497717|ensembl:ENSP00000497754|ensembl:ENSP00000497760|ensembl:ENSP00000497765|ensembl:ENSP00000497773|ensembl:ENSP00000498091|ensembl:ENSP00000343027|ensembl:ENSP00000391439|ensembl:ENSP00000496800|ensembl:ENSP00000496829|uniprotkb:P04898|uniprotkb:P11015|uniprotkb:P31871|uniprotkb:Q5U074|uniprotkb:Q8TAN5|uniprotkb:Q9UGA4|uniprotkb:A8KA88|uniprotkb:B4E2V1|uniprotkb:C9J3A4 intact:EBI-2928178|ensembl:ENSP00000354859|ensembl:ENSP00000442172|uniprotkb:Q9NZR3|uniprotkb:Q9UPA9 psi-mi:gnai1_human(display_long)|uniprotkb:GNAI1(gene name)|psi-mi:GNAI1(display_short)|uniprotkb:Adenylate cyclase-inhibiting G alpha protein(gene name synonym) psi-mi:drd2_human(display_long)|uniprotkb:DRD2(gene name)|psi-mi:DRD2(display_short)|uniprotkb:Dopamine D2 receptor(gene name synonym) psi-mi:"MI:0012"(bioluminescence resonance energy transfer) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-21300793|imex:IM-26955-31 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:2334"(luminescence donor) psi-mi:"MI:2335"(luminescence acceptor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) reactome:R-HSA-418597|reactome:R-HSA-422356|reactome:R-HSA-9009391|reactome:R-HSA-9660821|reactome:R-HSA-418594|ensembl:ENST00000351004(transcript)|ensembl:ENST00000442586(transcript)|ensembl:ENST00000648098(transcript)|ensembl:ENSG00000127955(gene)|ensembl:ENST00000648306(transcript)|ensembl:ENST00000648412(transcript)|ensembl:ENST00000648476(transcript)|ensembl:ENST00000648663(transcript)|ensembl:ENST00000648832(transcript)|ensembl:ENST00000648877(transcript)|ensembl:ENST00000648953(transcript)|ensembl:ENST00000649225(transcript)|ensembl:ENST00000649267(transcript)|ensembl:ENST00000649487(transcript)|ensembl:ENST00000649796(transcript)|ensembl:ENST00000649855(transcript)|go:"GO:0000287"(magnesium ion binding)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005765"(lysosomal membrane)|go:"GO:0005813"(centrosome)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0007049"(cell cycle)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007193"(adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway)|go:"GO:0019003"(GDP binding)|go:"GO:0030496"(midbody)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0031821"(G protein-coupled serotonin receptor binding)|go:"GO:0032794"(GTPase activating protein binding)|go:"GO:0043434"(response to peptide hormone)|go:"GO:0043949"(regulation of cAMP-mediated signaling)|go:"GO:0045121"(membrane raft)|go:"GO:0050805"(negative regulation of synaptic transmission)|go:"GO:0051301"(cell division)|go:"GO:0060236"(regulation of mitotic spindle organization)|go:"GO:0070062"(extracellular exosome)|go:"GO:0099738"(cell cortex region)|go:"GO:1904322"(cellular response to forskolin)|go:"GO:1904778"(positive regulation of protein localization to cell cortex)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR001408(G-protein alpha subunit, group I)|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|mint:P63096|rcsb pdb:1KJY|rcsb pdb:1Y3A|rcsb pdb:2G83|rcsb pdb:2GTP|rcsb pdb:2IK8|rcsb pdb:2OM2|rcsb pdb:2XNS|rcsb pdb:3ONW|rcsb pdb:3QE0|rcsb pdb:3QI2|rcsb pdb:3UMR|rcsb pdb:3UMS|rcsb pdb:4G5Q|rcsb pdb:5JS7|rcsb pdb:5JS8|rcsb pdb:5TDH|rcsb pdb:6CMO|rcsb pdb:6CRK|rcsb pdb:6DDE|rcsb pdb:6DDF|rcsb pdb:6KPF|rcsb pdb:6KPG|rcsb pdb:6LFM|rcsb pdb:6LFO|rcsb pdb:6LML|rcsb pdb:6N4B|rcsb pdb:6OMM|rcsb pdb:6OS9|rcsb pdb:6OSA|rcsb pdb:6OT0|rcsb pdb:6PB0|rcsb pdb:6PB1|rcsb pdb:6PT0|rcsb pdb:6QNO|rcsb pdb:6VU8|rcsb pdb:6XBJ|rcsb pdb:6XBK|rcsb pdb:6XBL|rcsb pdb:6XBM|rcsb pdb:7CMU|rcsb pdb:7CMV|rcsb pdb:7E2X|rcsb pdb:7E2Y|rcsb pdb:7E2Z|rcsb pdb:7E32|rcsb pdb:7E33|rcsb pdb:7E9G|rcsb pdb:7EB2|rcsb pdb:7JVR|rcsb pdb:7L0P|rcsb pdb:7L0Q|rcsb pdb:7L0R|rcsb pdb:7L0S|rcsb pdb:7O7F|reactome:R-HSA-170670|reactome:R-HSA-392170|reactome:R-HSA-400042|reactome:R-HSA-418555|refseq:NP_002060.4|refseq:NP_001243343.1 ensembl:ENSG00000149295(gene)|ensembl:ENST00000362072(transcript)|ensembl:ENST00000542968(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001591"(dopamine neurotransmitter receptor activity, coupled via Gi/Go)|go:"GO:0001659"(temperature homeostasis)|go:"GO:0001666"(response to hypoxia)|go:"GO:0001669"(acrosomal vesicle)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0001975"(response to amphetamine)|go:"GO:0001976"(nervous system process involved in regulation of systemic arterial blood pressure)|go:"GO:0002027"(regulation of heart rate)|go:"GO:0002028"(regulation of sodium ion transport)|go:"GO:0002031"(G protein-coupled receptor internalization)|go:"GO:0002052"(positive regulation of neuroblast proliferation)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005929"(cilium)|go:"GO:0006874"(cellular calcium ion homeostasis)|go:"GO:0006914"(autophagy)|go:"GO:0007195"(adenylate cyclase-inhibiting dopamine receptor signaling pathway)|go:"GO:0007270"(neuron-neuron synaptic transmission)|go:"GO:0007409"(axonogenesis)|go:"GO:0007416"(synapse assembly)|go:"GO:0007608"(sensory perception of smell)|go:"GO:0007616"(long-term memory)|go:"GO:0007625"(grooming behavior)|go:"GO:0007626"(locomotory behavior)|go:"GO:0007628"(adult walking behavior)|go:"GO:0008104"(protein localization)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0008306"(associative learning)|go:"GO:0008542"(visual learning)|go:"GO:0009416"(response to light stimulus)|go:"GO:0009636"(response to toxic substance)|go:"GO:0010039"(response to iron ion)|go:"GO:0014059"(regulation of dopamine secretion)|go:"GO:0014069"(postsynaptic density)|go:"GO:0014854"(response to inactivity)|go:"GO:0015459"(potassium channel regulator activity)|go:"GO:0016055"(Wnt signaling pathway)|go:"GO:0016328"(lateral plasma membrane)|go:"GO:0021756"(striatum development)|go:"GO:0021769"(orbitofrontal cortex development)|go:"GO:0021853"(cerebral cortex GABAergic interneuron migration)|go:"GO:0021984"(adenohypophysis development)|go:"GO:0030139"(endocytic vesicle)|go:"GO:0030336"(negative regulation of cell migration)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0030432"(peristalsis)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0031223"(auditory behavior)|go:"GO:0032147"(activation of protein kinase activity)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032467"(positive regulation of cytokinesis)|go:"GO:0007194"(negative regulation of adenylate cyclase activity)|go:"GO:0032922"(circadian regulation of gene expression)|go:"GO:0034776"(response to histamine)|go:"GO:0035094"(response to nicotine)|go:"GO:0035240"(dopamine binding)|go:"GO:0035255"(ionotropic glutamate receptor binding)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0035810"(positive regulation of urine volume)|go:"GO:0035815"(positive regulation of renal sodium excretion)|go:"GO:0036126"(sperm flagellum)|go:"GO:0040018"(positive regulation of multicellular organism growth)|go:"GO:0042220"(response to cocaine)|go:"GO:0042321"(negative regulation of circadian sleep/wake cycle, sleep)|go:"GO:0042417"(dopamine metabolic process)|go:"GO:0042493"|go:"GO:0042756"(drinking behavior)|go:"GO:0042802"(identical protein binding)|go:"GO:0043197"(dendritic spine)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0043204"(perikaryon)|go:"GO:0043266"(regulation of potassium ion transport)|go:"GO:0043278"(response to morphine)|go:"GO:0043473"(pigmentation)|go:"GO:0043666"(regulation of phosphoprotein phosphatase activity)|go:"GO:0043679"(axon terminus)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045202"(synapse)|go:"GO:0045745"(positive regulation of G protein-coupled receptor signaling pathway)|go:"GO:0045824"(negative regulation of innate immune response)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046676"(negative regulation of insulin secretion)|go:"GO:0046717"(acid secretion)|go:"GO:0048148"(behavioral response to cocaine)|go:"GO:0048149"(behavioral response to ethanol)|go:"GO:0048169"(regulation of long-term neuronal synaptic plasticity)|go:"GO:0048678"(response to axon injury)|go:"GO:0048755"(branching morphogenesis of a nerve)|go:"GO:0050482"(arachidonic acid secretion)|go:"GO:0050709"(negative regulation of protein secretion)|go:"GO:0051209"(release of sequestered calcium ion into cytosol)|go:"GO:0051481"(negative regulation of cytosolic calcium ion concentration)|go:"GO:0051482"(positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway)|go:"GO:0051584"(regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0051586"(positive regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0051898"(negative regulation of protein kinase B signaling)|go:"GO:0051967"(negative regulation of synaptic transmission, glutamatergic)|go:"GO:0060079"(excitatory postsynaptic potential)|go:"GO:0060124"(positive regulation of growth hormone secretion)|go:"GO:0060134"(prepulse inhibition)|go:"GO:0060158"(phospholipase C-activating dopamine receptor signaling pathway)|go:"GO:0060160"(negative regulation of dopamine receptor signaling pathway)|go:"GO:0060170"(ciliary membrane)|go:"GO:0060548"(negative regulation of cell death)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071880"(adenylate cyclase-activating adrenergic receptor signaling pathway)|go:"GO:0090325"(regulation of locomotion involved in locomotory behavior)|go:"GO:0097730"(non-motile cilium)|go:"GO:0098691"(dopaminergic synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099055"(integral component of postsynaptic membrane)|go:"GO:0099056"(integral component of presynaptic membrane)|go:"GO:0099170"(postsynaptic modulation of chemical synaptic transmission)|go:"GO:1900168"(positive regulation of glial cell-derived neurotrophic factor production)|go:"GO:1900273"(positive regulation of long-term synaptic potentiation)|go:"GO:1901386"(negative regulation of voltage-gated calcium channel activity)|go:"GO:1990384"(hyaloid vascular plexus regression)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000929(Dopamine receptor)|interpro:IPR001922(Dopamine D2 receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|mint:P14416|rcsb pdb:1I15|rcsb pdb:5AER|rcsb pdb:6CM4|rcsb pdb:6LUQ|rcsb pdb:6VMS|rcsb pdb:7JVR|reactome:R-HSA-390651|go:"GO:0051823"(regulation of synapse structural plasticity)|refseq:NP_000786.1|refseq:NP_057658.2|refseq:XP_016872785.1|dip:DIP-5977N - - - figure legend:Figure 3C, 4D, 5E/F, Supplementary fig 2C, 5B,6B|agonist:"Arachidonyl-2'-chloroethylamide (ACEA) - a selective, synthetic agonist of CB1R. In the presence of the CB1 blocking peptide (aas 300-431) agonist activity was reduced."|comment:Untagged CB1 was co-expressed.|agonist:"Quinpirole (D2L agonist). The presence of the blocking peptide did not significantly affect the action of Quinpirole."|comment:"A reduction in BRET Eff signals between Gai-Rluc and D2L-GFP 2 was observed in cells treated with 1 uM quinpirole and increasing concentrations of ACEA (Fig. 5E)."|antagonist:"Quinpirole (D2L agonist) added concurrently with Arachidonyl-2'-chloroethylamide (ACEA) - a selective, synthetic agonist of CB1R."|comment:Co-expression and co-activation of either CB1 and beta2 did not alter D2 coupling to G proteins.|comment:"PTx (Pertussis toxin) blocked Quinpirole-dependent and also ACEA+CB1 dependent increases in BRET Efficiency."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:3yQOCXJb8Undp/Xw42xrBiG5ePE9606 rogid:S5beCpwOapTeUXcS45WlU5ApKEI9606 rigid:XgEiULzcTxKPQmBRGjsoC3lxdyI false renilla luciferase protein tag:93-94 green fluorescent protein tag:c-c - - psi-mi:"MI:0866"(tag visualisation) psi-mi:"MI:0866"(tag visualisation) uniprotkb:P07550 uniprotkb:P63092-1 intact:EBI-491169|uniprotkb:Q9UH95|uniprotkb:Q9UHA1|uniprotkb:O14823|uniprotkb:O14824|uniprotkb:O14825|uniprotkb:O14826|uniprotkb:Q4JG18|uniprotkb:Q8NEQ9|uniprotkb:Q9UMZ5|uniprotkb:Q6P4D8|uniprotkb:B0LPE4|uniprotkb:B2R7X2|uniprotkb:Q53GA6|uniprotkb:Q6GMT4|uniprotkb:Q96EC3|uniprotkb:Q9UCZ0|uniprotkb:Q9UCZ1|uniprotkb:Q9UCZ2|uniprotkb:Q9UCZ3|ensembl:ENSP00000305372 intact:EBI-11428973|uniprotkb:P04895-1|ensembl:ENSP00000360126 psi-mi:adrb2_human(display_long)|uniprotkb:Beta-2 adrenoreceptor(gene name synonym)|uniprotkb:ADRB2(gene name)|psi-mi:ADRB2(display_short)|uniprotkb:ADRB2R(gene name synonym)|uniprotkb:B2AR(gene name synonym) psi-mi:p63092-1(display_long)|uniprotkb:Adenylate cyclase-stimulating G alpha protein(gene name synonym)|uniprotkb:GNAS(gene name)|psi-mi:GNAS(display_short)|uniprotkb:GNAS1(gene name synonym)|uniprotkb:GSP(gene name synonym)|uniprotkb:Alpha-S2(isoform synonym)|uniprotkb:GNASl(isoform synonym)|uniprotkb:Alpha-S-long(isoform synonym) psi-mi:"MI:0012"(bioluminescence resonance energy transfer) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-21300844|imex:IM-26955-33 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:2335"(luminescence acceptor) psi-mi:"MI:2334"(luminescence donor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_000015.1|ensembl:ENSG00000169252(gene)|ensembl:ENST00000305988(transcript)|go:"GO:0001540"(amyloid-beta binding)|go:"GO:0002024"(diet induced thermogenesis)|go:"GO:0002025"(norepinephrine-epinephrine-mediated vasodilation involved in regulation of systemic arterial blood pressure)|go:"GO:0002028"(regulation of sodium ion transport)|go:"GO:0002032"(desensitization of G protein-coupled receptor signaling pathway by arrestin)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004941"(beta2-adrenergic receptor activity)|go:"GO:0005634"(nucleus)|go:"GO:0005764"(lysosome)|go:"GO:0005768"(endosome)|go:"GO:0005769"(early endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0007166"(cell surface receptor signaling pathway)|go:"GO:0007171"(activation of transmembrane receptor protein tyrosine kinase activity)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007190"(activation of adenylate cyclase activity)|go:"GO:0008179"(adenylate cyclase binding)|go:"GO:0008333"(endosome to lysosome transport)|go:"GO:0009409"(response to cold)|go:"GO:0010008"(endosome membrane)|go:"GO:0010739"(positive regulation of protein kinase A signaling)|go:"GO:0015459"(potassium channel regulator activity)|go:"GO:0016020"(membrane)|go:"GO:0016324"(apical plasma membrane)|go:"GO:0030501"(positive regulation of bone mineralization)|go:"GO:0030669"(clathrin-coated endocytic vesicle membrane)|go:"GO:0031649"(heat generation)|go:"GO:0040015"(negative regulation of multicellular organism growth)|go:"GO:0042802"(identical protein binding)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0043235"(receptor complex)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045453"(bone resorption)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0045986"(negative regulation of smooth muscle contraction)|go:"GO:0050873"(brown fat cell differentiation)|go:"GO:0061885"(positive regulation of mini excitatory postsynaptic potential)|go:"GO:0071875"(adrenergic receptor signaling pathway)|go:"GO:0071880"(adenylate cyclase-activating adrenergic receptor signaling pathway)|go:"GO:0051380"(norepinephrine binding)|go:"GO:0071902"(positive regulation of protein serine/threonine kinase activity)|go:"GO:0120162"(positive regulation of cold-induced thermogenesis)|go:"GO:1901098"(positive regulation of autophagosome maturation)|go:"GO:1904504"(positive regulation of lipophagy)|go:"GO:1904646"(cellular response to amyloid-beta)|go:"GO:1990911"(response to psychosocial stress)|go:"GO:2000481"(positive regulation of cAMP-dependent protein kinase activity)|go:"GO:2000969"(positive regulation of AMPA receptor activity)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000332(Adrenergic receptor, beta 2)|interpro:IPR002233(Adrenergic receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|mint:P07550|rcsb pdb:1GQ4|rcsb pdb:2R4R|rcsb pdb:2R4S|rcsb pdb:2RH1|rcsb pdb:3D4S|rcsb pdb:3KJ6|rcsb pdb:3NY8|rcsb pdb:3NY9|rcsb pdb:3NYA|rcsb pdb:3P0G|rcsb pdb:3PDS|rcsb pdb:3SN6|rcsb pdb:4GBR|rcsb pdb:4LDE|rcsb pdb:4LDL|rcsb pdb:4LDO|rcsb pdb:4QKX|rcsb pdb:5D5A|rcsb pdb:5D5B|rcsb pdb:5D6L|rcsb pdb:5JQH|rcsb pdb:5X7D|rcsb pdb:6E67|rcsb pdb:6KR8|rcsb pdb:6MXT|rcsb pdb:6N48|rcsb pdb:6NI3|rcsb pdb:6OBA|rcsb pdb:6PRZ|rcsb pdb:6PS0|rcsb pdb:6PS1|rcsb pdb:6PS2|rcsb pdb:6PS3|rcsb pdb:6PS4|rcsb pdb:6PS5|rcsb pdb:6PS6|rcsb pdb:7BZ2|rcsb pdb:7DHI|rcsb pdb:7DHR|reactome:R-HSA-390696|reactome:R-HSA-418555|reactome:R-HSA-5689880|reactome:R-HSA-8856825|reactome:R-HSA-8856828|reactome:R-HSA-9660821|dip:DIP-33948N intact:EBI-1047114(isoform-parent)|ensembl:ENSG00000087460(gene)|ensembl:ENST00000371085(transcript) - - - figure legend:Supplementary Fig 4A/B +6B/D|agonist:isoprenaline - a Beta2 agonist|antagonist:"Cholera toxin (CTx)"|comment:Co-expression and co-activation of CB1 did not alter coupling to G proteins.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:+pZmEUm9SKroeqpa7uI44Bz18zo9606 rogid:fUnrdxwx4bBAqE3iO2cCB3UInyg9606 rigid:5UQZGP9JtcqjyfF2pERO8swN0L8 false green fluorescent protein tag:?-? renilla luciferase protein tag:188-189 - - psi-mi:"MI:0866"(tag visualisation) psi-mi:"MI:0866"(tag visualisation) uniprotkb:P63092-1 uniprotkb:P21554 intact:EBI-11428973|uniprotkb:P04895-1|ensembl:ENSP00000360126 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 psi-mi:p63092-1(display_long)|uniprotkb:Adenylate cyclase-stimulating G alpha protein(gene name synonym)|uniprotkb:GNAS(gene name)|psi-mi:GNAS(display_short)|uniprotkb:GNAS1(gene name synonym)|uniprotkb:GSP(gene name synonym)|uniprotkb:Alpha-S2(isoform synonym)|uniprotkb:GNASl(isoform synonym)|uniprotkb:Alpha-S-long(isoform synonym) psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:"MI:0012"(bioluminescence resonance energy transfer) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-21303281|imex:IM-26955-35 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:2334"(luminescence donor) psi-mi:"MI:2335"(luminescence acceptor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-1047114(isoform-parent)|ensembl:ENSG00000087460(gene)|ensembl:ENST00000371085(transcript) go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N - - - figure legend:Fig 6B/C, Suppl. Fig. 2B,6B|comment:Untagged D2L was co-expressed.|agonist:Co-application of ACEA and quinpirole. Application of either compound alone had marginal effect.|comment:Co-expression and co-activation of either D2L and beta2 did not alter CB1 or D2 coupling to G proteins.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:fUnrdxwx4bBAqE3iO2cCB3UInyg9606 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rigid:ikE1S4Z4c0LoQhiyy9K6oRrNWfU false renilla luciferase protein tag:188-189 green fluorescent protein tag:c-c - - psi-mi:"MI:0866"(tag visualisation) psi-mi:"MI:0866"(tag visualisation) uniprotkb:P63092-1 uniprotkb:P14416 intact:EBI-11428973|uniprotkb:P04895-1|ensembl:ENSP00000360126 intact:EBI-2928178|ensembl:ENSP00000354859|ensembl:ENSP00000442172|uniprotkb:Q9NZR3|uniprotkb:Q9UPA9 psi-mi:p63092-1(display_long)|uniprotkb:Adenylate cyclase-stimulating G alpha protein(gene name synonym)|uniprotkb:GNAS(gene name)|psi-mi:GNAS(display_short)|uniprotkb:GNAS1(gene name synonym)|uniprotkb:GSP(gene name synonym)|uniprotkb:Alpha-S2(isoform synonym)|uniprotkb:GNASl(isoform synonym)|uniprotkb:Alpha-S-long(isoform synonym) psi-mi:drd2_human(display_long)|uniprotkb:DRD2(gene name)|psi-mi:DRD2(display_short)|uniprotkb:Dopamine D2 receptor(gene name synonym) psi-mi:"MI:0012"(bioluminescence resonance energy transfer) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-21303292|imex:IM-26955-11 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:2334"(luminescence donor) psi-mi:"MI:2335"(luminescence acceptor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-1047114(isoform-parent)|ensembl:ENSG00000087460(gene)|ensembl:ENST00000371085(transcript) ensembl:ENSG00000149295(gene)|ensembl:ENST00000362072(transcript)|ensembl:ENST00000542968(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001591"(dopamine neurotransmitter receptor activity, coupled via Gi/Go)|go:"GO:0001659"(temperature homeostasis)|go:"GO:0001666"(response to hypoxia)|go:"GO:0001669"(acrosomal vesicle)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0001975"(response to amphetamine)|go:"GO:0001976"(nervous system process involved in regulation of systemic arterial blood pressure)|go:"GO:0002027"(regulation of heart rate)|go:"GO:0002028"(regulation of sodium ion transport)|go:"GO:0002031"(G protein-coupled receptor internalization)|go:"GO:0002052"(positive regulation of neuroblast proliferation)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005929"(cilium)|go:"GO:0006874"(cellular calcium ion homeostasis)|go:"GO:0006914"(autophagy)|go:"GO:0007195"(adenylate cyclase-inhibiting dopamine receptor signaling pathway)|go:"GO:0007270"(neuron-neuron synaptic transmission)|go:"GO:0007409"(axonogenesis)|go:"GO:0007416"(synapse assembly)|go:"GO:0007608"(sensory perception of smell)|go:"GO:0007616"(long-term memory)|go:"GO:0007625"(grooming behavior)|go:"GO:0007626"(locomotory behavior)|go:"GO:0007628"(adult walking behavior)|go:"GO:0008104"(protein localization)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0008306"(associative learning)|go:"GO:0008542"(visual learning)|go:"GO:0009416"(response to light stimulus)|go:"GO:0009636"(response to toxic substance)|go:"GO:0010039"(response to iron ion)|go:"GO:0014059"(regulation of dopamine secretion)|go:"GO:0014069"(postsynaptic density)|go:"GO:0014854"(response to inactivity)|go:"GO:0015459"(potassium channel regulator activity)|go:"GO:0016055"(Wnt signaling pathway)|go:"GO:0016328"(lateral plasma membrane)|go:"GO:0021756"(striatum development)|go:"GO:0021769"(orbitofrontal cortex development)|go:"GO:0021853"(cerebral cortex GABAergic interneuron migration)|go:"GO:0021984"(adenohypophysis development)|go:"GO:0030139"(endocytic vesicle)|go:"GO:0030336"(negative regulation of cell migration)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0030432"(peristalsis)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0031223"(auditory behavior)|go:"GO:0032147"(activation of protein kinase activity)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032467"(positive regulation of cytokinesis)|go:"GO:0007194"(negative regulation of adenylate cyclase activity)|go:"GO:0032922"(circadian regulation of gene expression)|go:"GO:0034776"(response to histamine)|go:"GO:0035094"(response to nicotine)|go:"GO:0035240"(dopamine binding)|go:"GO:0035255"(ionotropic glutamate receptor binding)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0035810"(positive regulation of urine volume)|go:"GO:0035815"(positive regulation of renal sodium excretion)|go:"GO:0036126"(sperm flagellum)|go:"GO:0040018"(positive regulation of multicellular organism growth)|go:"GO:0042220"(response to cocaine)|go:"GO:0042321"(negative regulation of circadian sleep/wake cycle, sleep)|go:"GO:0042417"(dopamine metabolic process)|go:"GO:0042493"|go:"GO:0042756"(drinking behavior)|go:"GO:0042802"(identical protein binding)|go:"GO:0043197"(dendritic spine)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0043204"(perikaryon)|go:"GO:0043266"(regulation of potassium ion transport)|go:"GO:0043278"(response to morphine)|go:"GO:0043473"(pigmentation)|go:"GO:0043666"(regulation of phosphoprotein phosphatase activity)|go:"GO:0043679"(axon terminus)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045202"(synapse)|go:"GO:0045745"(positive regulation of G protein-coupled receptor signaling pathway)|go:"GO:0045824"(negative regulation of innate immune response)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046676"(negative regulation of insulin secretion)|go:"GO:0046717"(acid secretion)|go:"GO:0048148"(behavioral response to cocaine)|go:"GO:0048149"(behavioral response to ethanol)|go:"GO:0048169"(regulation of long-term neuronal synaptic plasticity)|go:"GO:0048678"(response to axon injury)|go:"GO:0048755"(branching morphogenesis of a nerve)|go:"GO:0050482"(arachidonic acid secretion)|go:"GO:0050709"(negative regulation of protein secretion)|go:"GO:0051209"(release of sequestered calcium ion into cytosol)|go:"GO:0051481"(negative regulation of cytosolic calcium ion concentration)|go:"GO:0051482"(positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway)|go:"GO:0051584"(regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0051586"(positive regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0051898"(negative regulation of protein kinase B signaling)|go:"GO:0051967"(negative regulation of synaptic transmission, glutamatergic)|go:"GO:0060079"(excitatory postsynaptic potential)|go:"GO:0060124"(positive regulation of growth hormone secretion)|go:"GO:0060134"(prepulse inhibition)|go:"GO:0060158"(phospholipase C-activating dopamine receptor signaling pathway)|go:"GO:0060160"(negative regulation of dopamine receptor signaling pathway)|go:"GO:0060170"(ciliary membrane)|go:"GO:0060548"(negative regulation of cell death)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071880"(adenylate cyclase-activating adrenergic receptor signaling pathway)|go:"GO:0090325"(regulation of locomotion involved in locomotory behavior)|go:"GO:0097730"(non-motile cilium)|go:"GO:0098691"(dopaminergic synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099055"(integral component of postsynaptic membrane)|go:"GO:0099056"(integral component of presynaptic membrane)|go:"GO:0099170"(postsynaptic modulation of chemical synaptic transmission)|go:"GO:1900168"(positive regulation of glial cell-derived neurotrophic factor production)|go:"GO:1900273"(positive regulation of long-term synaptic potentiation)|go:"GO:1901386"(negative regulation of voltage-gated calcium channel activity)|go:"GO:1990384"(hyaloid vascular plexus regression)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000929(Dopamine receptor)|interpro:IPR001922(Dopamine D2 receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|mint:P14416|rcsb pdb:1I15|rcsb pdb:5AER|rcsb pdb:6CM4|rcsb pdb:6LUQ|rcsb pdb:6VMS|rcsb pdb:7JVR|reactome:R-HSA-390651|go:"GO:0051823"(regulation of synapse structural plasticity)|refseq:NP_000786.1|refseq:NP_057658.2|refseq:XP_016872785.1|dip:DIP-5977N - - - figure legend:Fig 6E/F , Suppl. Fig. 2D, 6D|comment:Untagged CB1 was co-expressed.|agonist:Co-application of ACEA and quinpirole. Application of either compound alone had marginal effect.|comment:Co-expression and co-activation of either CB1 and beta2 did not alter D2 coupling to G proteins.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:fUnrdxwx4bBAqE3iO2cCB3UInyg9606 rogid:S5beCpwOapTeUXcS45WlU5ApKEI9606 rigid:U7qaJo09UnaaD1RWbqF8BTc1/EQ false renilla luciferase protein tag:188-189 green fluorescent protein tag:c-c - - psi-mi:"MI:0866"(tag visualisation) psi-mi:"MI:0866"(tag visualisation) uniprotkb:P29066 uniprotkb:P21554 intact:EBI-4303019|ensembl:ENSRNOP00000046069 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 psi-mi:arrb1_rat(display_long)|uniprotkb:Arrb1(gene name)|psi-mi:Arrb1(display_short)|uniprotkb:Arrestin beta-1(gene name synonym) psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:"MI:0012"(bioluminescence resonance energy transfer) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-21303315|imex:IM-26955-40 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:2334"(luminescence donor) psi-mi:"MI:2335"(luminescence acceptor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:2156|refseq:NP_037042.1|interpro:IPR011022(Arrestin-like, C-terminal)|interpro:IPR014752(Arrestin, C-terminal)|interpro:IPR014753(Arrestin, N-terminal)|interpro:IPR014756(Immunoglobulin E-set)|interpro:IPR017864|mint:P29066|rcsb pdb:4JQI|rcsb pdb:6KL7|rcsb pdb:6U1N|reactome:R-RNO-418555|reactome:R-RNO-432720|reactome:R-RNO-432722|reactome:R-RNO-456926|reactome:R-RNO-5635838|reactome:R-RNO-5674135|reactome:R-RNO-5689880|reactome:R-RNO-8856825|reactome:R-RNO-8856828|interpro:IPR011021(Arrestin-like, N-terminal)|ensembl:ENSRNOG00000030404(gene)|ensembl:ENSRNOT00000043554(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0000785"(chromatin)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0001934"(positive regulation of protein phosphorylation)|go:"GO:0002031"(G protein-coupled receptor internalization)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0003713"(transcription coactivator activity)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005159"(insulin-like growth factor receptor binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005768"(endosome)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0006897"(endocytosis)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007602"(phototransduction)|go:"GO:0008277"(regulation of G protein-coupled receptor signaling pathway)|go:"GO:0008284"(positive regulation of cell population proliferation)|go:"GO:0014069"(postsynaptic density)|go:"GO:0015031"(protein transport)|go:"GO:0016323"(basolateral plasma membrane)|go:"GO:0016567"(protein ubiquitination)|go:"GO:0016573"(histone acetylation)|go:"GO:0016604"(nuclear body)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0019899"(enzyme binding)|go:"GO:0030276"(clathrin binding)|go:"GO:0030331"(estrogen receptor binding)|go:"GO:0031143"(pseudopodium)|go:"GO:0031397"(negative regulation of protein ubiquitination)|go:"GO:0031398"(positive regulation of protein ubiquitination)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031434"(mitogen-activated protein kinase kinase binding)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0031691"(alpha-1A adrenergic receptor binding)|go:"GO:0031692"(alpha-1B adrenergic receptor binding)|go:"GO:0031701"(angiotensin receptor binding)|go:"GO:0031762"(follicle-stimulating hormone receptor binding)|go:"GO:0031896"(V2 vasopressin receptor binding)|go:"GO:0032088"(negative regulation of NF-kappaB transcription factor activity)|go:"GO:0032092"(positive regulation of protein binding)|go:"GO:0032715"(negative regulation of interleukin-6 production)|go:"GO:0032717"(negative regulation of interleukin-8 production)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|go:"GO:0034260"(negative regulation of GTPase activity)|go:"GO:0034393"(positive regulation of smooth muscle cell apoptotic process)|go:"GO:0035025"(positive regulation of Rho protein signal transduction)|go:"GO:0035066"(positive regulation of histone acetylation)|go:"GO:0035612"(AP-2 adaptor complex binding)|go:"GO:0035615"(clathrin adaptor activity)|go:"GO:0035774"(positive regulation of insulin secretion involved in cellular response to glucose stimulus)|go:"GO:0042493"|go:"GO:0042699"(follicle-stimulating hormone signaling pathway)|go:"GO:0042981"(regulation of apoptotic process)|go:"GO:0043027"(cysteine-type endopeptidase inhibitor activity involved in apoptotic process)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0043149"(stress fiber assembly)|go:"GO:0043154"(negative regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0043161"(proteasome-mediated ubiquitin-dependent protein catabolic process)|go:"GO:0043197"(dendritic spine)|go:"GO:0043280"(positive regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0043524"(negative regulation of neuron apoptotic process)|go:"GO:0044325"(transmembrane transporter binding)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0045309"(protein phosphorylated amino acid binding)|go:"GO:0045746"(negative regulation of Notch signaling pathway)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0050975"(sensory perception of touch)|go:"GO:0051219"(phosphoprotein binding)|go:"GO:0070373"(negative regulation of ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0090240"(positive regulation of histone H4 acetylation)|go:"GO:1990763"(arrestin family protein binding)|interpro:IPR000698(Arrestin)|dip:DIP-40808N go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N - - - figure legend:Figure 9, 11B.|agonist:"Arachidonyl-2'-chloroethylamide (ACEA) - a selective, synthetic agonist of CB1R."|comment:Untagged D2L was co-expressed.|agonist:"Quinpirole (D2L agonist)."|comment:Co-application of both ACEA and quinpirole significantly potentiated BRET Eff signal between Beta-arrestin1-Rluc and CB 1-GFP compared to ACEA-treated cells.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:154Z++W8VsgmEd4rSCEVkw4xLgA10116 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rigid:6qKo3JcqNG6Ajzd0aaJlBBi8zFY false renilla luciferase protein tag:c-c green fluorescent protein tag:c-c - - psi-mi:"MI:0866"(tag visualisation) psi-mi:"MI:0866"(tag visualisation) uniprotkb:P29066 uniprotkb:P14416 intact:EBI-4303019|ensembl:ENSRNOP00000046069 intact:EBI-2928178|ensembl:ENSP00000354859|ensembl:ENSP00000442172|uniprotkb:Q9NZR3|uniprotkb:Q9UPA9 psi-mi:arrb1_rat(display_long)|uniprotkb:Arrb1(gene name)|psi-mi:Arrb1(display_short)|uniprotkb:Arrestin beta-1(gene name synonym) psi-mi:drd2_human(display_long)|uniprotkb:DRD2(gene name)|psi-mi:DRD2(display_short)|uniprotkb:Dopamine D2 receptor(gene name synonym) psi-mi:"MI:0012"(bioluminescence resonance energy transfer) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-21303492|imex:IM-26955-42 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:2335"(luminescence acceptor) psi-mi:"MI:2334"(luminescence donor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:2156|refseq:NP_037042.1|interpro:IPR011022(Arrestin-like, C-terminal)|interpro:IPR014752(Arrestin, C-terminal)|interpro:IPR014753(Arrestin, N-terminal)|interpro:IPR014756(Immunoglobulin E-set)|interpro:IPR017864|mint:P29066|rcsb pdb:4JQI|rcsb pdb:6KL7|rcsb pdb:6U1N|reactome:R-RNO-418555|reactome:R-RNO-432720|reactome:R-RNO-432722|reactome:R-RNO-456926|reactome:R-RNO-5635838|reactome:R-RNO-5674135|reactome:R-RNO-5689880|reactome:R-RNO-8856825|reactome:R-RNO-8856828|interpro:IPR011021(Arrestin-like, N-terminal)|ensembl:ENSRNOG00000030404(gene)|ensembl:ENSRNOT00000043554(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0000785"(chromatin)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0001934"(positive regulation of protein phosphorylation)|go:"GO:0002031"(G protein-coupled receptor internalization)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0003713"(transcription coactivator activity)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005159"(insulin-like growth factor receptor binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005768"(endosome)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0006897"(endocytosis)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007602"(phototransduction)|go:"GO:0008277"(regulation of G protein-coupled receptor signaling pathway)|go:"GO:0008284"(positive regulation of cell population proliferation)|go:"GO:0014069"(postsynaptic density)|go:"GO:0015031"(protein transport)|go:"GO:0016323"(basolateral plasma membrane)|go:"GO:0016567"(protein ubiquitination)|go:"GO:0016573"(histone acetylation)|go:"GO:0016604"(nuclear body)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0019899"(enzyme binding)|go:"GO:0030276"(clathrin binding)|go:"GO:0030331"(estrogen receptor binding)|go:"GO:0031143"(pseudopodium)|go:"GO:0031397"(negative regulation of protein ubiquitination)|go:"GO:0031398"(positive regulation of protein ubiquitination)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031434"(mitogen-activated protein kinase kinase binding)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0031691"(alpha-1A adrenergic receptor binding)|go:"GO:0031692"(alpha-1B adrenergic receptor binding)|go:"GO:0031701"(angiotensin receptor binding)|go:"GO:0031762"(follicle-stimulating hormone receptor binding)|go:"GO:0031896"(V2 vasopressin receptor binding)|go:"GO:0032088"(negative regulation of NF-kappaB transcription factor activity)|go:"GO:0032092"(positive regulation of protein binding)|go:"GO:0032715"(negative regulation of interleukin-6 production)|go:"GO:0032717"(negative regulation of interleukin-8 production)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|go:"GO:0034260"(negative regulation of GTPase activity)|go:"GO:0034393"(positive regulation of smooth muscle cell apoptotic process)|go:"GO:0035025"(positive regulation of Rho protein signal transduction)|go:"GO:0035066"(positive regulation of histone acetylation)|go:"GO:0035612"(AP-2 adaptor complex binding)|go:"GO:0035615"(clathrin adaptor activity)|go:"GO:0035774"(positive regulation of insulin secretion involved in cellular response to glucose stimulus)|go:"GO:0042493"|go:"GO:0042699"(follicle-stimulating hormone signaling pathway)|go:"GO:0042981"(regulation of apoptotic process)|go:"GO:0043027"(cysteine-type endopeptidase inhibitor activity involved in apoptotic process)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0043149"(stress fiber assembly)|go:"GO:0043154"(negative regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0043161"(proteasome-mediated ubiquitin-dependent protein catabolic process)|go:"GO:0043197"(dendritic spine)|go:"GO:0043280"(positive regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0043524"(negative regulation of neuron apoptotic process)|go:"GO:0044325"(transmembrane transporter binding)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0045309"(protein phosphorylated amino acid binding)|go:"GO:0045746"(negative regulation of Notch signaling pathway)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0050975"(sensory perception of touch)|go:"GO:0051219"(phosphoprotein binding)|go:"GO:0070373"(negative regulation of ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0090240"(positive regulation of histone H4 acetylation)|go:"GO:1990763"(arrestin family protein binding)|interpro:IPR000698(Arrestin)|dip:DIP-40808N ensembl:ENSG00000149295(gene)|ensembl:ENST00000362072(transcript)|ensembl:ENST00000542968(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001591"(dopamine neurotransmitter receptor activity, coupled via Gi/Go)|go:"GO:0001659"(temperature homeostasis)|go:"GO:0001666"(response to hypoxia)|go:"GO:0001669"(acrosomal vesicle)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0001975"(response to amphetamine)|go:"GO:0001976"(nervous system process involved in regulation of systemic arterial blood pressure)|go:"GO:0002027"(regulation of heart rate)|go:"GO:0002028"(regulation of sodium ion transport)|go:"GO:0002031"(G protein-coupled receptor internalization)|go:"GO:0002052"(positive regulation of neuroblast proliferation)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005929"(cilium)|go:"GO:0006874"(cellular calcium ion homeostasis)|go:"GO:0006914"(autophagy)|go:"GO:0007195"(adenylate cyclase-inhibiting dopamine receptor signaling pathway)|go:"GO:0007270"(neuron-neuron synaptic transmission)|go:"GO:0007409"(axonogenesis)|go:"GO:0007416"(synapse assembly)|go:"GO:0007608"(sensory perception of smell)|go:"GO:0007616"(long-term memory)|go:"GO:0007625"(grooming behavior)|go:"GO:0007626"(locomotory behavior)|go:"GO:0007628"(adult walking behavior)|go:"GO:0008104"(protein localization)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0008306"(associative learning)|go:"GO:0008542"(visual learning)|go:"GO:0009416"(response to light stimulus)|go:"GO:0009636"(response to toxic substance)|go:"GO:0010039"(response to iron ion)|go:"GO:0014059"(regulation of dopamine secretion)|go:"GO:0014069"(postsynaptic density)|go:"GO:0014854"(response to inactivity)|go:"GO:0015459"(potassium channel regulator activity)|go:"GO:0016055"(Wnt signaling pathway)|go:"GO:0016328"(lateral plasma membrane)|go:"GO:0021756"(striatum development)|go:"GO:0021769"(orbitofrontal cortex development)|go:"GO:0021853"(cerebral cortex GABAergic interneuron migration)|go:"GO:0021984"(adenohypophysis development)|go:"GO:0030139"(endocytic vesicle)|go:"GO:0030336"(negative regulation of cell migration)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0030432"(peristalsis)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0031223"(auditory behavior)|go:"GO:0032147"(activation of protein kinase activity)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032467"(positive regulation of cytokinesis)|go:"GO:0007194"(negative regulation of adenylate cyclase activity)|go:"GO:0032922"(circadian regulation of gene expression)|go:"GO:0034776"(response to histamine)|go:"GO:0035094"(response to nicotine)|go:"GO:0035240"(dopamine binding)|go:"GO:0035255"(ionotropic glutamate receptor binding)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0035810"(positive regulation of urine volume)|go:"GO:0035815"(positive regulation of renal sodium excretion)|go:"GO:0036126"(sperm flagellum)|go:"GO:0040018"(positive regulation of multicellular organism growth)|go:"GO:0042220"(response to cocaine)|go:"GO:0042321"(negative regulation of circadian sleep/wake cycle, sleep)|go:"GO:0042417"(dopamine metabolic process)|go:"GO:0042493"|go:"GO:0042756"(drinking behavior)|go:"GO:0042802"(identical protein binding)|go:"GO:0043197"(dendritic spine)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0043204"(perikaryon)|go:"GO:0043266"(regulation of potassium ion transport)|go:"GO:0043278"(response to morphine)|go:"GO:0043473"(pigmentation)|go:"GO:0043666"(regulation of phosphoprotein phosphatase activity)|go:"GO:0043679"(axon terminus)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045202"(synapse)|go:"GO:0045745"(positive regulation of G protein-coupled receptor signaling pathway)|go:"GO:0045824"(negative regulation of innate immune response)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046676"(negative regulation of insulin secretion)|go:"GO:0046717"(acid secretion)|go:"GO:0048148"(behavioral response to cocaine)|go:"GO:0048149"(behavioral response to ethanol)|go:"GO:0048169"(regulation of long-term neuronal synaptic plasticity)|go:"GO:0048678"(response to axon injury)|go:"GO:0048755"(branching morphogenesis of a nerve)|go:"GO:0050482"(arachidonic acid secretion)|go:"GO:0050709"(negative regulation of protein secretion)|go:"GO:0051209"(release of sequestered calcium ion into cytosol)|go:"GO:0051481"(negative regulation of cytosolic calcium ion concentration)|go:"GO:0051482"(positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway)|go:"GO:0051584"(regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0051586"(positive regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0051898"(negative regulation of protein kinase B signaling)|go:"GO:0051967"(negative regulation of synaptic transmission, glutamatergic)|go:"GO:0060079"(excitatory postsynaptic potential)|go:"GO:0060124"(positive regulation of growth hormone secretion)|go:"GO:0060134"(prepulse inhibition)|go:"GO:0060158"(phospholipase C-activating dopamine receptor signaling pathway)|go:"GO:0060160"(negative regulation of dopamine receptor signaling pathway)|go:"GO:0060170"(ciliary membrane)|go:"GO:0060548"(negative regulation of cell death)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071880"(adenylate cyclase-activating adrenergic receptor signaling pathway)|go:"GO:0090325"(regulation of locomotion involved in locomotory behavior)|go:"GO:0097730"(non-motile cilium)|go:"GO:0098691"(dopaminergic synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099055"(integral component of postsynaptic membrane)|go:"GO:0099056"(integral component of presynaptic membrane)|go:"GO:0099170"(postsynaptic modulation of chemical synaptic transmission)|go:"GO:1900168"(positive regulation of glial cell-derived neurotrophic factor production)|go:"GO:1900273"(positive regulation of long-term synaptic potentiation)|go:"GO:1901386"(negative regulation of voltage-gated calcium channel activity)|go:"GO:1990384"(hyaloid vascular plexus regression)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000929(Dopamine receptor)|interpro:IPR001922(Dopamine D2 receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|mint:P14416|rcsb pdb:1I15|rcsb pdb:5AER|rcsb pdb:6CM4|rcsb pdb:6LUQ|rcsb pdb:6VMS|rcsb pdb:7JVR|reactome:R-HSA-390651|go:"GO:0051823"(regulation of synapse structural plasticity)|refseq:NP_000786.1|refseq:NP_057658.2|refseq:XP_016872785.1|dip:DIP-5977N - - - figure legend:Figure 10, 11D, Supplementary Figure 8B|agonist:"Arachidonyl-2'-chloroethylamide (ACEA) - a selective, synthetic agonist of CB1R."|comment:Untagged CB1 was co-expressed.|agonist:"Quinpirole (D2L agonist)."|comment:Co-application of both ACEA and quinpirole significantly increased BRET Eff signal as compared to BRET Eff signals obtained from cells treated with each agonist individually.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:154Z++W8VsgmEd4rSCEVkw4xLgA10116 rogid:S5beCpwOapTeUXcS45WlU5ApKEI9606 rigid:O60Ve2WWzDCug8FG08mnb2VhXvE false green fluorescent protein tag:c-c renilla luciferase protein tag:c-c - - psi-mi:"MI:0866"(tag visualisation) psi-mi:"MI:0866"(tag visualisation) uniprotkb:P29066 uniprotkb:P21554 intact:EBI-4303019|ensembl:ENSRNOP00000046069 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 psi-mi:arrb1_rat(display_long)|uniprotkb:Arrb1(gene name)|psi-mi:Arrb1(display_short)|uniprotkb:Arrestin beta-1(gene name synonym) psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:"MI:0012"(bioluminescence resonance energy transfer) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-21303504|imex:IM-26955-17 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0941"(competitor) psi-mi:"MI:2334"(luminescence donor) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:2156|refseq:NP_037042.1|interpro:IPR011022(Arrestin-like, C-terminal)|interpro:IPR014752(Arrestin, C-terminal)|interpro:IPR014753(Arrestin, N-terminal)|interpro:IPR014756(Immunoglobulin E-set)|interpro:IPR017864|mint:P29066|rcsb pdb:4JQI|rcsb pdb:6KL7|rcsb pdb:6U1N|reactome:R-RNO-418555|reactome:R-RNO-432720|reactome:R-RNO-432722|reactome:R-RNO-456926|reactome:R-RNO-5635838|reactome:R-RNO-5674135|reactome:R-RNO-5689880|reactome:R-RNO-8856825|reactome:R-RNO-8856828|interpro:IPR011021(Arrestin-like, N-terminal)|ensembl:ENSRNOG00000030404(gene)|ensembl:ENSRNOT00000043554(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0000785"(chromatin)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0001934"(positive regulation of protein phosphorylation)|go:"GO:0002031"(G protein-coupled receptor internalization)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0003713"(transcription coactivator activity)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005159"(insulin-like growth factor receptor binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005768"(endosome)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0006897"(endocytosis)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007602"(phototransduction)|go:"GO:0008277"(regulation of G protein-coupled receptor signaling pathway)|go:"GO:0008284"(positive regulation of cell population proliferation)|go:"GO:0014069"(postsynaptic density)|go:"GO:0015031"(protein transport)|go:"GO:0016323"(basolateral plasma membrane)|go:"GO:0016567"(protein ubiquitination)|go:"GO:0016573"(histone acetylation)|go:"GO:0016604"(nuclear body)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0019899"(enzyme binding)|go:"GO:0030276"(clathrin binding)|go:"GO:0030331"(estrogen receptor binding)|go:"GO:0031143"(pseudopodium)|go:"GO:0031397"(negative regulation of protein ubiquitination)|go:"GO:0031398"(positive regulation of protein ubiquitination)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031434"(mitogen-activated protein kinase kinase binding)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0031691"(alpha-1A adrenergic receptor binding)|go:"GO:0031692"(alpha-1B adrenergic receptor binding)|go:"GO:0031701"(angiotensin receptor binding)|go:"GO:0031762"(follicle-stimulating hormone receptor binding)|go:"GO:0031896"(V2 vasopressin receptor binding)|go:"GO:0032088"(negative regulation of NF-kappaB transcription factor activity)|go:"GO:0032092"(positive regulation of protein binding)|go:"GO:0032715"(negative regulation of interleukin-6 production)|go:"GO:0032717"(negative regulation of interleukin-8 production)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|go:"GO:0034260"(negative regulation of GTPase activity)|go:"GO:0034393"(positive regulation of smooth muscle cell apoptotic process)|go:"GO:0035025"(positive regulation of Rho protein signal transduction)|go:"GO:0035066"(positive regulation of histone acetylation)|go:"GO:0035612"(AP-2 adaptor complex binding)|go:"GO:0035615"(clathrin adaptor activity)|go:"GO:0035774"(positive regulation of insulin secretion involved in cellular response to glucose stimulus)|go:"GO:0042493"|go:"GO:0042699"(follicle-stimulating hormone signaling pathway)|go:"GO:0042981"(regulation of apoptotic process)|go:"GO:0043027"(cysteine-type endopeptidase inhibitor activity involved in apoptotic process)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0043149"(stress fiber assembly)|go:"GO:0043154"(negative regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0043161"(proteasome-mediated ubiquitin-dependent protein catabolic process)|go:"GO:0043197"(dendritic spine)|go:"GO:0043280"(positive regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0043524"(negative regulation of neuron apoptotic process)|go:"GO:0044325"(transmembrane transporter binding)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0045309"(protein phosphorylated amino acid binding)|go:"GO:0045746"(negative regulation of Notch signaling pathway)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0050975"(sensory perception of touch)|go:"GO:0051219"(phosphoprotein binding)|go:"GO:0070373"(negative regulation of ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0090240"(positive regulation of histone H4 acetylation)|go:"GO:1990763"(arrestin family protein binding)|interpro:IPR000698(Arrestin)|dip:DIP-40808N go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N - - - figure legend:Figure 11B|agonist:"Arachidonyl-2'-chloroethylamide (ACEA) - a selective, synthetic agonist of CB1R."|comment:Untagged D2L was co-expressed.|agonist:"Quinpirole (D2L agonist)."|comment:"In cells expressing CB1-BP lower energy transfer was observed between cells treated with quinpirole or co-treated with quinpirole and ACEA, as compared to cells treated with the same agonist(s) expressing an empty pcDNA. No change in the energy transfer was observed in cells expressing CB1-BP following ACEA treatment compared to cells transfected with empty pcDNA."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:154Z++W8VsgmEd4rSCEVkw4xLgA10116 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rigid:6qKo3JcqNG6Ajzd0aaJlBBi8zFY false renilla luciferase protein tag:c-c sufficient binding region:432-456 - - psi-mi:"MI:0866"(tag visualisation) psi-mi:"MI:0866"(tag visualisation) uniprotkb:P29066 uniprotkb:P21554 intact:EBI-4303019|ensembl:ENSRNOP00000046069 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 psi-mi:arrb1_rat(display_long)|uniprotkb:Arrb1(gene name)|psi-mi:Arrb1(display_short)|uniprotkb:Arrestin beta-1(gene name synonym) psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:"MI:0012"(bioluminescence resonance energy transfer) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-21303504|imex:IM-26955-17 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:2334"(luminescence donor) psi-mi:"MI:2335"(luminescence acceptor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:2156|refseq:NP_037042.1|interpro:IPR011022(Arrestin-like, C-terminal)|interpro:IPR014752(Arrestin, C-terminal)|interpro:IPR014753(Arrestin, N-terminal)|interpro:IPR014756(Immunoglobulin E-set)|interpro:IPR017864|mint:P29066|rcsb pdb:4JQI|rcsb pdb:6KL7|rcsb pdb:6U1N|reactome:R-RNO-418555|reactome:R-RNO-432720|reactome:R-RNO-432722|reactome:R-RNO-456926|reactome:R-RNO-5635838|reactome:R-RNO-5674135|reactome:R-RNO-5689880|reactome:R-RNO-8856825|reactome:R-RNO-8856828|interpro:IPR011021(Arrestin-like, N-terminal)|ensembl:ENSRNOG00000030404(gene)|ensembl:ENSRNOT00000043554(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0000785"(chromatin)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0001934"(positive regulation of protein phosphorylation)|go:"GO:0002031"(G protein-coupled receptor internalization)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0003713"(transcription coactivator activity)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005159"(insulin-like growth factor receptor binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005768"(endosome)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0006897"(endocytosis)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007602"(phototransduction)|go:"GO:0008277"(regulation of G protein-coupled receptor signaling pathway)|go:"GO:0008284"(positive regulation of cell population proliferation)|go:"GO:0014069"(postsynaptic density)|go:"GO:0015031"(protein transport)|go:"GO:0016323"(basolateral plasma membrane)|go:"GO:0016567"(protein ubiquitination)|go:"GO:0016573"(histone acetylation)|go:"GO:0016604"(nuclear body)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0019899"(enzyme binding)|go:"GO:0030276"(clathrin binding)|go:"GO:0030331"(estrogen receptor binding)|go:"GO:0031143"(pseudopodium)|go:"GO:0031397"(negative regulation of protein ubiquitination)|go:"GO:0031398"(positive regulation of protein ubiquitination)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031434"(mitogen-activated protein kinase kinase binding)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0031691"(alpha-1A adrenergic receptor binding)|go:"GO:0031692"(alpha-1B adrenergic receptor binding)|go:"GO:0031701"(angiotensin receptor binding)|go:"GO:0031762"(follicle-stimulating hormone receptor binding)|go:"GO:0031896"(V2 vasopressin receptor binding)|go:"GO:0032088"(negative regulation of NF-kappaB transcription factor activity)|go:"GO:0032092"(positive regulation of protein binding)|go:"GO:0032715"(negative regulation of interleukin-6 production)|go:"GO:0032717"(negative regulation of interleukin-8 production)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|go:"GO:0034260"(negative regulation of GTPase activity)|go:"GO:0034393"(positive regulation of smooth muscle cell apoptotic process)|go:"GO:0035025"(positive regulation of Rho protein signal transduction)|go:"GO:0035066"(positive regulation of histone acetylation)|go:"GO:0035612"(AP-2 adaptor complex binding)|go:"GO:0035615"(clathrin adaptor activity)|go:"GO:0035774"(positive regulation of insulin secretion involved in cellular response to glucose stimulus)|go:"GO:0042493"|go:"GO:0042699"(follicle-stimulating hormone signaling pathway)|go:"GO:0042981"(regulation of apoptotic process)|go:"GO:0043027"(cysteine-type endopeptidase inhibitor activity involved in apoptotic process)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0043149"(stress fiber assembly)|go:"GO:0043154"(negative regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0043161"(proteasome-mediated ubiquitin-dependent protein catabolic process)|go:"GO:0043197"(dendritic spine)|go:"GO:0043280"(positive regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0043524"(negative regulation of neuron apoptotic process)|go:"GO:0044325"(transmembrane transporter binding)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0045309"(protein phosphorylated amino acid binding)|go:"GO:0045746"(negative regulation of Notch signaling pathway)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0050975"(sensory perception of touch)|go:"GO:0051219"(phosphoprotein binding)|go:"GO:0070373"(negative regulation of ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0090240"(positive regulation of histone H4 acetylation)|go:"GO:1990763"(arrestin family protein binding)|interpro:IPR000698(Arrestin)|dip:DIP-40808N go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N - - - figure legend:Figure 11B|agonist:"Arachidonyl-2'-chloroethylamide (ACEA) - a selective, synthetic agonist of CB1R."|comment:Untagged D2L was co-expressed.|agonist:"Quinpirole (D2L agonist)."|comment:"In cells expressing CB1-BP lower energy transfer was observed between cells treated with quinpirole or co-treated with quinpirole and ACEA, as compared to cells treated with the same agonist(s) expressing an empty pcDNA. No change in the energy transfer was observed in cells expressing CB1-BP following ACEA treatment compared to cells transfected with empty pcDNA."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:154Z++W8VsgmEd4rSCEVkw4xLgA10116 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rigid:6qKo3JcqNG6Ajzd0aaJlBBi8zFY false renilla luciferase protein tag:c-c green fluorescent protein tag:c-c - - psi-mi:"MI:0866"(tag visualisation) psi-mi:"MI:0866"(tag visualisation) uniprotkb:P29066 uniprotkb:P21554 intact:EBI-4303019|ensembl:ENSRNOP00000046069 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 psi-mi:arrb1_rat(display_long)|uniprotkb:Arrb1(gene name)|psi-mi:Arrb1(display_short)|uniprotkb:Arrestin beta-1(gene name synonym) psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:"MI:0012"(bioluminescence resonance energy transfer) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-21303519|imex:IM-26955-19 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0941"(competitor) psi-mi:"MI:2335"(luminescence acceptor) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:2156|refseq:NP_037042.1|interpro:IPR011022(Arrestin-like, C-terminal)|interpro:IPR014752(Arrestin, C-terminal)|interpro:IPR014753(Arrestin, N-terminal)|interpro:IPR014756(Immunoglobulin E-set)|interpro:IPR017864|mint:P29066|rcsb pdb:4JQI|rcsb pdb:6KL7|rcsb pdb:6U1N|reactome:R-RNO-418555|reactome:R-RNO-432720|reactome:R-RNO-432722|reactome:R-RNO-456926|reactome:R-RNO-5635838|reactome:R-RNO-5674135|reactome:R-RNO-5689880|reactome:R-RNO-8856825|reactome:R-RNO-8856828|interpro:IPR011021(Arrestin-like, N-terminal)|ensembl:ENSRNOG00000030404(gene)|ensembl:ENSRNOT00000043554(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0000785"(chromatin)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0001934"(positive regulation of protein phosphorylation)|go:"GO:0002031"(G protein-coupled receptor internalization)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0003713"(transcription coactivator activity)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005159"(insulin-like growth factor receptor binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005768"(endosome)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0006897"(endocytosis)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007602"(phototransduction)|go:"GO:0008277"(regulation of G protein-coupled receptor signaling pathway)|go:"GO:0008284"(positive regulation of cell population proliferation)|go:"GO:0014069"(postsynaptic density)|go:"GO:0015031"(protein transport)|go:"GO:0016323"(basolateral plasma membrane)|go:"GO:0016567"(protein ubiquitination)|go:"GO:0016573"(histone acetylation)|go:"GO:0016604"(nuclear body)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0019899"(enzyme binding)|go:"GO:0030276"(clathrin binding)|go:"GO:0030331"(estrogen receptor binding)|go:"GO:0031143"(pseudopodium)|go:"GO:0031397"(negative regulation of protein ubiquitination)|go:"GO:0031398"(positive regulation of protein ubiquitination)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031434"(mitogen-activated protein kinase kinase binding)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0031691"(alpha-1A adrenergic receptor binding)|go:"GO:0031692"(alpha-1B adrenergic receptor binding)|go:"GO:0031701"(angiotensin receptor binding)|go:"GO:0031762"(follicle-stimulating hormone receptor binding)|go:"GO:0031896"(V2 vasopressin receptor binding)|go:"GO:0032088"(negative regulation of NF-kappaB transcription factor activity)|go:"GO:0032092"(positive regulation of protein binding)|go:"GO:0032715"(negative regulation of interleukin-6 production)|go:"GO:0032717"(negative regulation of interleukin-8 production)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|go:"GO:0034260"(negative regulation of GTPase activity)|go:"GO:0034393"(positive regulation of smooth muscle cell apoptotic process)|go:"GO:0035025"(positive regulation of Rho protein signal transduction)|go:"GO:0035066"(positive regulation of histone acetylation)|go:"GO:0035612"(AP-2 adaptor complex binding)|go:"GO:0035615"(clathrin adaptor activity)|go:"GO:0035774"(positive regulation of insulin secretion involved in cellular response to glucose stimulus)|go:"GO:0042493"|go:"GO:0042699"(follicle-stimulating hormone signaling pathway)|go:"GO:0042981"(regulation of apoptotic process)|go:"GO:0043027"(cysteine-type endopeptidase inhibitor activity involved in apoptotic process)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0043149"(stress fiber assembly)|go:"GO:0043154"(negative regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0043161"(proteasome-mediated ubiquitin-dependent protein catabolic process)|go:"GO:0043197"(dendritic spine)|go:"GO:0043280"(positive regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0043524"(negative regulation of neuron apoptotic process)|go:"GO:0044325"(transmembrane transporter binding)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0045309"(protein phosphorylated amino acid binding)|go:"GO:0045746"(negative regulation of Notch signaling pathway)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0050975"(sensory perception of touch)|go:"GO:0051219"(phosphoprotein binding)|go:"GO:0070373"(negative regulation of ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0090240"(positive regulation of histone H4 acetylation)|go:"GO:1990763"(arrestin family protein binding)|interpro:IPR000698(Arrestin)|dip:DIP-40808N go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N - - - figure legend:Figure 11D|agonist:"Arachidonyl-2'-chloroethylamide (ACEA) - a selective, synthetic agonist of CB1R."|comment:Untagged CB1 was co-expressed.|agonist:"Quinpirole (D2L agonist)."|comment:Co-expression of CB1-BP reduced the BRET signal in cells treated with ACEA or ACEA plus Quinpirole. Treating the cells with quinpirole alone did not alter BRET signal in cells expressing CB1-BP compared to those expressing empty pcDNA.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:154Z++W8VsgmEd4rSCEVkw4xLgA10116 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rigid:ZNsx0flJfcVWTNoBW1X/WakwRY4 false green fluorescent protein tag:c-c sufficient binding region:432-456 - - psi-mi:"MI:0866"(tag visualisation) psi-mi:"MI:0866"(tag visualisation) uniprotkb:P29066 uniprotkb:P14416 intact:EBI-4303019|ensembl:ENSRNOP00000046069 intact:EBI-2928178|ensembl:ENSP00000354859|ensembl:ENSP00000442172|uniprotkb:Q9NZR3|uniprotkb:Q9UPA9 psi-mi:arrb1_rat(display_long)|uniprotkb:Arrb1(gene name)|psi-mi:Arrb1(display_short)|uniprotkb:Arrestin beta-1(gene name synonym) psi-mi:drd2_human(display_long)|uniprotkb:DRD2(gene name)|psi-mi:DRD2(display_short)|uniprotkb:Dopamine D2 receptor(gene name synonym) psi-mi:"MI:0012"(bioluminescence resonance energy transfer) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-21303519|imex:IM-26955-19 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:2335"(luminescence acceptor) psi-mi:"MI:2334"(luminescence donor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:2156|refseq:NP_037042.1|interpro:IPR011022(Arrestin-like, C-terminal)|interpro:IPR014752(Arrestin, C-terminal)|interpro:IPR014753(Arrestin, N-terminal)|interpro:IPR014756(Immunoglobulin E-set)|interpro:IPR017864|mint:P29066|rcsb pdb:4JQI|rcsb pdb:6KL7|rcsb pdb:6U1N|reactome:R-RNO-418555|reactome:R-RNO-432720|reactome:R-RNO-432722|reactome:R-RNO-456926|reactome:R-RNO-5635838|reactome:R-RNO-5674135|reactome:R-RNO-5689880|reactome:R-RNO-8856825|reactome:R-RNO-8856828|interpro:IPR011021(Arrestin-like, N-terminal)|ensembl:ENSRNOG00000030404(gene)|ensembl:ENSRNOT00000043554(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0000785"(chromatin)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0001934"(positive regulation of protein phosphorylation)|go:"GO:0002031"(G protein-coupled receptor internalization)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0003713"(transcription coactivator activity)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005159"(insulin-like growth factor receptor binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005768"(endosome)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0006897"(endocytosis)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007602"(phototransduction)|go:"GO:0008277"(regulation of G protein-coupled receptor signaling pathway)|go:"GO:0008284"(positive regulation of cell population proliferation)|go:"GO:0014069"(postsynaptic density)|go:"GO:0015031"(protein transport)|go:"GO:0016323"(basolateral plasma membrane)|go:"GO:0016567"(protein ubiquitination)|go:"GO:0016573"(histone acetylation)|go:"GO:0016604"(nuclear body)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0019899"(enzyme binding)|go:"GO:0030276"(clathrin binding)|go:"GO:0030331"(estrogen receptor binding)|go:"GO:0031143"(pseudopodium)|go:"GO:0031397"(negative regulation of protein ubiquitination)|go:"GO:0031398"(positive regulation of protein ubiquitination)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031434"(mitogen-activated protein kinase kinase binding)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0031691"(alpha-1A adrenergic receptor binding)|go:"GO:0031692"(alpha-1B adrenergic receptor binding)|go:"GO:0031701"(angiotensin receptor binding)|go:"GO:0031762"(follicle-stimulating hormone receptor binding)|go:"GO:0031896"(V2 vasopressin receptor binding)|go:"GO:0032088"(negative regulation of NF-kappaB transcription factor activity)|go:"GO:0032092"(positive regulation of protein binding)|go:"GO:0032715"(negative regulation of interleukin-6 production)|go:"GO:0032717"(negative regulation of interleukin-8 production)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|go:"GO:0034260"(negative regulation of GTPase activity)|go:"GO:0034393"(positive regulation of smooth muscle cell apoptotic process)|go:"GO:0035025"(positive regulation of Rho protein signal transduction)|go:"GO:0035066"(positive regulation of histone acetylation)|go:"GO:0035612"(AP-2 adaptor complex binding)|go:"GO:0035615"(clathrin adaptor activity)|go:"GO:0035774"(positive regulation of insulin secretion involved in cellular response to glucose stimulus)|go:"GO:0042493"|go:"GO:0042699"(follicle-stimulating hormone signaling pathway)|go:"GO:0042981"(regulation of apoptotic process)|go:"GO:0043027"(cysteine-type endopeptidase inhibitor activity involved in apoptotic process)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0043149"(stress fiber assembly)|go:"GO:0043154"(negative regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0043161"(proteasome-mediated ubiquitin-dependent protein catabolic process)|go:"GO:0043197"(dendritic spine)|go:"GO:0043280"(positive regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0043524"(negative regulation of neuron apoptotic process)|go:"GO:0044325"(transmembrane transporter binding)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0045309"(protein phosphorylated amino acid binding)|go:"GO:0045746"(negative regulation of Notch signaling pathway)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0050975"(sensory perception of touch)|go:"GO:0051219"(phosphoprotein binding)|go:"GO:0070373"(negative regulation of ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0090240"(positive regulation of histone H4 acetylation)|go:"GO:1990763"(arrestin family protein binding)|interpro:IPR000698(Arrestin)|dip:DIP-40808N ensembl:ENSG00000149295(gene)|ensembl:ENST00000362072(transcript)|ensembl:ENST00000542968(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001591"(dopamine neurotransmitter receptor activity, coupled via Gi/Go)|go:"GO:0001659"(temperature homeostasis)|go:"GO:0001666"(response to hypoxia)|go:"GO:0001669"(acrosomal vesicle)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0001975"(response to amphetamine)|go:"GO:0001976"(nervous system process involved in regulation of systemic arterial blood pressure)|go:"GO:0002027"(regulation of heart rate)|go:"GO:0002028"(regulation of sodium ion transport)|go:"GO:0002031"(G protein-coupled receptor internalization)|go:"GO:0002052"(positive regulation of neuroblast proliferation)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005929"(cilium)|go:"GO:0006874"(cellular calcium ion homeostasis)|go:"GO:0006914"(autophagy)|go:"GO:0007195"(adenylate cyclase-inhibiting dopamine receptor signaling pathway)|go:"GO:0007270"(neuron-neuron synaptic transmission)|go:"GO:0007409"(axonogenesis)|go:"GO:0007416"(synapse assembly)|go:"GO:0007608"(sensory perception of smell)|go:"GO:0007616"(long-term memory)|go:"GO:0007625"(grooming behavior)|go:"GO:0007626"(locomotory behavior)|go:"GO:0007628"(adult walking behavior)|go:"GO:0008104"(protein localization)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0008306"(associative learning)|go:"GO:0008542"(visual learning)|go:"GO:0009416"(response to light stimulus)|go:"GO:0009636"(response to toxic substance)|go:"GO:0010039"(response to iron ion)|go:"GO:0014059"(regulation of dopamine secretion)|go:"GO:0014069"(postsynaptic density)|go:"GO:0014854"(response to inactivity)|go:"GO:0015459"(potassium channel regulator activity)|go:"GO:0016055"(Wnt signaling pathway)|go:"GO:0016328"(lateral plasma membrane)|go:"GO:0021756"(striatum development)|go:"GO:0021769"(orbitofrontal cortex development)|go:"GO:0021853"(cerebral cortex GABAergic interneuron migration)|go:"GO:0021984"(adenohypophysis development)|go:"GO:0030139"(endocytic vesicle)|go:"GO:0030336"(negative regulation of cell migration)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0030432"(peristalsis)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0031223"(auditory behavior)|go:"GO:0032147"(activation of protein kinase activity)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032467"(positive regulation of cytokinesis)|go:"GO:0007194"(negative regulation of adenylate cyclase activity)|go:"GO:0032922"(circadian regulation of gene expression)|go:"GO:0034776"(response to histamine)|go:"GO:0035094"(response to nicotine)|go:"GO:0035240"(dopamine binding)|go:"GO:0035255"(ionotropic glutamate receptor binding)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0035810"(positive regulation of urine volume)|go:"GO:0035815"(positive regulation of renal sodium excretion)|go:"GO:0036126"(sperm flagellum)|go:"GO:0040018"(positive regulation of multicellular organism growth)|go:"GO:0042220"(response to cocaine)|go:"GO:0042321"(negative regulation of circadian sleep/wake cycle, sleep)|go:"GO:0042417"(dopamine metabolic process)|go:"GO:0042493"|go:"GO:0042756"(drinking behavior)|go:"GO:0042802"(identical protein binding)|go:"GO:0043197"(dendritic spine)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0043204"(perikaryon)|go:"GO:0043266"(regulation of potassium ion transport)|go:"GO:0043278"(response to morphine)|go:"GO:0043473"(pigmentation)|go:"GO:0043666"(regulation of phosphoprotein phosphatase activity)|go:"GO:0043679"(axon terminus)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045202"(synapse)|go:"GO:0045745"(positive regulation of G protein-coupled receptor signaling pathway)|go:"GO:0045824"(negative regulation of innate immune response)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046676"(negative regulation of insulin secretion)|go:"GO:0046717"(acid secretion)|go:"GO:0048148"(behavioral response to cocaine)|go:"GO:0048149"(behavioral response to ethanol)|go:"GO:0048169"(regulation of long-term neuronal synaptic plasticity)|go:"GO:0048678"(response to axon injury)|go:"GO:0048755"(branching morphogenesis of a nerve)|go:"GO:0050482"(arachidonic acid secretion)|go:"GO:0050709"(negative regulation of protein secretion)|go:"GO:0051209"(release of sequestered calcium ion into cytosol)|go:"GO:0051481"(negative regulation of cytosolic calcium ion concentration)|go:"GO:0051482"(positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway)|go:"GO:0051584"(regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0051586"(positive regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0051898"(negative regulation of protein kinase B signaling)|go:"GO:0051967"(negative regulation of synaptic transmission, glutamatergic)|go:"GO:0060079"(excitatory postsynaptic potential)|go:"GO:0060124"(positive regulation of growth hormone secretion)|go:"GO:0060134"(prepulse inhibition)|go:"GO:0060158"(phospholipase C-activating dopamine receptor signaling pathway)|go:"GO:0060160"(negative regulation of dopamine receptor signaling pathway)|go:"GO:0060170"(ciliary membrane)|go:"GO:0060548"(negative regulation of cell death)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071880"(adenylate cyclase-activating adrenergic receptor signaling pathway)|go:"GO:0090325"(regulation of locomotion involved in locomotory behavior)|go:"GO:0097730"(non-motile cilium)|go:"GO:0098691"(dopaminergic synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099055"(integral component of postsynaptic membrane)|go:"GO:0099056"(integral component of presynaptic membrane)|go:"GO:0099170"(postsynaptic modulation of chemical synaptic transmission)|go:"GO:1900168"(positive regulation of glial cell-derived neurotrophic factor production)|go:"GO:1900273"(positive regulation of long-term synaptic potentiation)|go:"GO:1901386"(negative regulation of voltage-gated calcium channel activity)|go:"GO:1990384"(hyaloid vascular plexus regression)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000929(Dopamine receptor)|interpro:IPR001922(Dopamine D2 receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|mint:P14416|rcsb pdb:1I15|rcsb pdb:5AER|rcsb pdb:6CM4|rcsb pdb:6LUQ|rcsb pdb:6VMS|rcsb pdb:7JVR|reactome:R-HSA-390651|go:"GO:0051823"(regulation of synapse structural plasticity)|refseq:NP_000786.1|refseq:NP_057658.2|refseq:XP_016872785.1|dip:DIP-5977N - - - figure legend:Figure 11D|agonist:"Arachidonyl-2'-chloroethylamide (ACEA) - a selective, synthetic agonist of CB1R."|comment:Untagged CB1 was co-expressed.|agonist:"Quinpirole (D2L agonist)."|comment:Co-expression of CB1-BP reduced the BRET signal in cells treated with ACEA or ACEA plus Quinpirole. Treating the cells with quinpirole alone did not alter BRET signal in cells expressing CB1-BP compared to those expressing empty pcDNA.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:154Z++W8VsgmEd4rSCEVkw4xLgA10116 rogid:S5beCpwOapTeUXcS45WlU5ApKEI9606 rigid:ZNsx0flJfcVWTNoBW1X/WakwRY4 false green fluorescent protein tag:c-c renilla luciferase protein tag:c-c - - psi-mi:"MI:0866"(tag visualisation) psi-mi:"MI:0866"(tag visualisation) uniprotkb:P21554 uniprotkb:P21554 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:"MI:0012"(bioluminescence resonance energy transfer) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-21305425|imex:IM-26955-44 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0941"(competitor) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:2335"(luminescence acceptor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N - - - figure legend:Supplementary Figure 5A|agonist:"Arachidonyl-2'-chloroethylamide (ACEA) - a selective, synthetic agonist of CB1R. In the presence of the blocking peptide agonist activity was not affected."|comment:Untagged D2L was co-expressed.|agonist:"Quinpirole (D2L agonist). The presence of the blocking peptide reduced the activity of Quinpirole."|comment:"The co-expression of CB1/D2L hetero-oligomer blocking peptide (CB1-BP), which inhibited the physical interaction between CB1 and D2 (Fig. 2C), inhibited the switch of CB1 and D2L coupling from Ga i to Gas proteins following co-activation of both receptors (Suppl. Fig. 5A,B)."|agonist:"ACEA plus Quinpirole (D2L agonist):-in the presence of CB1 blocking peptide the BRET signal increased."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rigid:IzLXYnd5auXbNl36x+xrzzb18Bw false sufficient binding region:432-456 green fluorescent protein tag:c-c - - psi-mi:"MI:0866"(tag visualisation) psi-mi:"MI:0866"(tag visualisation) uniprotkb:P21554 uniprotkb:P63096 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 intact:EBI-618639|ensembl:ENSP00000497051|ensembl:ENSP00000497179|ensembl:ENSP00000497260|ensembl:ENSP00000497315|ensembl:ENSP00000497379|ensembl:ENSP00000497717|ensembl:ENSP00000497754|ensembl:ENSP00000497760|ensembl:ENSP00000497765|ensembl:ENSP00000497773|ensembl:ENSP00000498091|ensembl:ENSP00000343027|ensembl:ENSP00000391439|ensembl:ENSP00000496800|ensembl:ENSP00000496829|uniprotkb:P04898|uniprotkb:P11015|uniprotkb:P31871|uniprotkb:Q5U074|uniprotkb:Q8TAN5|uniprotkb:Q9UGA4|uniprotkb:A8KA88|uniprotkb:B4E2V1|uniprotkb:C9J3A4 psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:gnai1_human(display_long)|uniprotkb:GNAI1(gene name)|psi-mi:GNAI1(display_short)|uniprotkb:Adenylate cyclase-inhibiting G alpha protein(gene name synonym) psi-mi:"MI:0012"(bioluminescence resonance energy transfer) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-21305425|imex:IM-26955-44 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0941"(competitor) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:2334"(luminescence donor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N reactome:R-HSA-418597|reactome:R-HSA-422356|reactome:R-HSA-9009391|reactome:R-HSA-9660821|reactome:R-HSA-418594|ensembl:ENST00000351004(transcript)|ensembl:ENST00000442586(transcript)|ensembl:ENST00000648098(transcript)|ensembl:ENSG00000127955(gene)|ensembl:ENST00000648306(transcript)|ensembl:ENST00000648412(transcript)|ensembl:ENST00000648476(transcript)|ensembl:ENST00000648663(transcript)|ensembl:ENST00000648832(transcript)|ensembl:ENST00000648877(transcript)|ensembl:ENST00000648953(transcript)|ensembl:ENST00000649225(transcript)|ensembl:ENST00000649267(transcript)|ensembl:ENST00000649487(transcript)|ensembl:ENST00000649796(transcript)|ensembl:ENST00000649855(transcript)|go:"GO:0000287"(magnesium ion binding)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005765"(lysosomal membrane)|go:"GO:0005813"(centrosome)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0007049"(cell cycle)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007193"(adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway)|go:"GO:0019003"(GDP binding)|go:"GO:0030496"(midbody)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0031821"(G protein-coupled serotonin receptor binding)|go:"GO:0032794"(GTPase activating protein binding)|go:"GO:0043434"(response to peptide hormone)|go:"GO:0043949"(regulation of cAMP-mediated signaling)|go:"GO:0045121"(membrane raft)|go:"GO:0050805"(negative regulation of synaptic transmission)|go:"GO:0051301"(cell division)|go:"GO:0060236"(regulation of mitotic spindle organization)|go:"GO:0070062"(extracellular exosome)|go:"GO:0099738"(cell cortex region)|go:"GO:1904322"(cellular response to forskolin)|go:"GO:1904778"(positive regulation of protein localization to cell cortex)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR001408(G-protein alpha subunit, group I)|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|mint:P63096|rcsb pdb:1KJY|rcsb pdb:1Y3A|rcsb pdb:2G83|rcsb pdb:2GTP|rcsb pdb:2IK8|rcsb pdb:2OM2|rcsb pdb:2XNS|rcsb pdb:3ONW|rcsb pdb:3QE0|rcsb pdb:3QI2|rcsb pdb:3UMR|rcsb pdb:3UMS|rcsb pdb:4G5Q|rcsb pdb:5JS7|rcsb pdb:5JS8|rcsb pdb:5TDH|rcsb pdb:6CMO|rcsb pdb:6CRK|rcsb pdb:6DDE|rcsb pdb:6DDF|rcsb pdb:6KPF|rcsb pdb:6KPG|rcsb pdb:6LFM|rcsb pdb:6LFO|rcsb pdb:6LML|rcsb pdb:6N4B|rcsb pdb:6OMM|rcsb pdb:6OS9|rcsb pdb:6OSA|rcsb pdb:6OT0|rcsb pdb:6PB0|rcsb pdb:6PB1|rcsb pdb:6PT0|rcsb pdb:6QNO|rcsb pdb:6VU8|rcsb pdb:6XBJ|rcsb pdb:6XBK|rcsb pdb:6XBL|rcsb pdb:6XBM|rcsb pdb:7CMU|rcsb pdb:7CMV|rcsb pdb:7E2X|rcsb pdb:7E2Y|rcsb pdb:7E2Z|rcsb pdb:7E32|rcsb pdb:7E33|rcsb pdb:7E9G|rcsb pdb:7EB2|rcsb pdb:7JVR|rcsb pdb:7L0P|rcsb pdb:7L0Q|rcsb pdb:7L0R|rcsb pdb:7L0S|rcsb pdb:7O7F|reactome:R-HSA-170670|reactome:R-HSA-392170|reactome:R-HSA-400042|reactome:R-HSA-418555|refseq:NP_002060.4|refseq:NP_001243343.1 - - - figure legend:Supplementary Figure 5A|agonist:"Arachidonyl-2'-chloroethylamide (ACEA) - a selective, synthetic agonist of CB1R. In the presence of the blocking peptide agonist activity was not affected."|comment:Untagged D2L was co-expressed.|agonist:"Quinpirole (D2L agonist). The presence of the blocking peptide reduced the activity of Quinpirole."|comment:"The co-expression of CB1/D2L hetero-oligomer blocking peptide (CB1-BP), which inhibited the physical interaction between CB1 and D2 (Fig. 2C), inhibited the switch of CB1 and D2L coupling from Ga i to Gas proteins following co-activation of both receptors (Suppl. Fig. 5A,B)."|agonist:"ACEA plus Quinpirole (D2L agonist):-in the presence of CB1 blocking peptide the BRET signal increased."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rogid:3yQOCXJb8Undp/Xw42xrBiG5ePE9606 rigid:IzLXYnd5auXbNl36x+xrzzb18Bw false sufficient binding region:432-456 renilla luciferase protein tag:93-94 - - psi-mi:"MI:0866"(tag visualisation) psi-mi:"MI:0866"(tag visualisation) uniprotkb:P63096 uniprotkb:P14416 intact:EBI-618639|ensembl:ENSP00000497051|ensembl:ENSP00000497179|ensembl:ENSP00000497260|ensembl:ENSP00000497315|ensembl:ENSP00000497379|ensembl:ENSP00000497717|ensembl:ENSP00000497754|ensembl:ENSP00000497760|ensembl:ENSP00000497765|ensembl:ENSP00000497773|ensembl:ENSP00000498091|ensembl:ENSP00000343027|ensembl:ENSP00000391439|ensembl:ENSP00000496800|ensembl:ENSP00000496829|uniprotkb:P04898|uniprotkb:P11015|uniprotkb:P31871|uniprotkb:Q5U074|uniprotkb:Q8TAN5|uniprotkb:Q9UGA4|uniprotkb:A8KA88|uniprotkb:B4E2V1|uniprotkb:C9J3A4 intact:EBI-2928178|ensembl:ENSP00000354859|ensembl:ENSP00000442172|uniprotkb:Q9NZR3|uniprotkb:Q9UPA9 psi-mi:gnai1_human(display_long)|uniprotkb:GNAI1(gene name)|psi-mi:GNAI1(display_short)|uniprotkb:Adenylate cyclase-inhibiting G alpha protein(gene name synonym) psi-mi:drd2_human(display_long)|uniprotkb:DRD2(gene name)|psi-mi:DRD2(display_short)|uniprotkb:Dopamine D2 receptor(gene name synonym) psi-mi:"MI:0012"(bioluminescence resonance energy transfer) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-21305447|imex:IM-26955-46 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:2334"(luminescence donor) psi-mi:"MI:2335"(luminescence acceptor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) reactome:R-HSA-418597|reactome:R-HSA-422356|reactome:R-HSA-9009391|reactome:R-HSA-9660821|reactome:R-HSA-418594|ensembl:ENST00000351004(transcript)|ensembl:ENST00000442586(transcript)|ensembl:ENST00000648098(transcript)|ensembl:ENSG00000127955(gene)|ensembl:ENST00000648306(transcript)|ensembl:ENST00000648412(transcript)|ensembl:ENST00000648476(transcript)|ensembl:ENST00000648663(transcript)|ensembl:ENST00000648832(transcript)|ensembl:ENST00000648877(transcript)|ensembl:ENST00000648953(transcript)|ensembl:ENST00000649225(transcript)|ensembl:ENST00000649267(transcript)|ensembl:ENST00000649487(transcript)|ensembl:ENST00000649796(transcript)|ensembl:ENST00000649855(transcript)|go:"GO:0000287"(magnesium ion binding)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005765"(lysosomal membrane)|go:"GO:0005813"(centrosome)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0007049"(cell cycle)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007193"(adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway)|go:"GO:0019003"(GDP binding)|go:"GO:0030496"(midbody)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0031821"(G protein-coupled serotonin receptor binding)|go:"GO:0032794"(GTPase activating protein binding)|go:"GO:0043434"(response to peptide hormone)|go:"GO:0043949"(regulation of cAMP-mediated signaling)|go:"GO:0045121"(membrane raft)|go:"GO:0050805"(negative regulation of synaptic transmission)|go:"GO:0051301"(cell division)|go:"GO:0060236"(regulation of mitotic spindle organization)|go:"GO:0070062"(extracellular exosome)|go:"GO:0099738"(cell cortex region)|go:"GO:1904322"(cellular response to forskolin)|go:"GO:1904778"(positive regulation of protein localization to cell cortex)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR001408(G-protein alpha subunit, group I)|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|mint:P63096|rcsb pdb:1KJY|rcsb pdb:1Y3A|rcsb pdb:2G83|rcsb pdb:2GTP|rcsb pdb:2IK8|rcsb pdb:2OM2|rcsb pdb:2XNS|rcsb pdb:3ONW|rcsb pdb:3QE0|rcsb pdb:3QI2|rcsb pdb:3UMR|rcsb pdb:3UMS|rcsb pdb:4G5Q|rcsb pdb:5JS7|rcsb pdb:5JS8|rcsb pdb:5TDH|rcsb pdb:6CMO|rcsb pdb:6CRK|rcsb pdb:6DDE|rcsb pdb:6DDF|rcsb pdb:6KPF|rcsb pdb:6KPG|rcsb pdb:6LFM|rcsb pdb:6LFO|rcsb pdb:6LML|rcsb pdb:6N4B|rcsb pdb:6OMM|rcsb pdb:6OS9|rcsb pdb:6OSA|rcsb pdb:6OT0|rcsb pdb:6PB0|rcsb pdb:6PB1|rcsb pdb:6PT0|rcsb pdb:6QNO|rcsb pdb:6VU8|rcsb pdb:6XBJ|rcsb pdb:6XBK|rcsb pdb:6XBL|rcsb pdb:6XBM|rcsb pdb:7CMU|rcsb pdb:7CMV|rcsb pdb:7E2X|rcsb pdb:7E2Y|rcsb pdb:7E2Z|rcsb pdb:7E32|rcsb pdb:7E33|rcsb pdb:7E9G|rcsb pdb:7EB2|rcsb pdb:7JVR|rcsb pdb:7L0P|rcsb pdb:7L0Q|rcsb pdb:7L0R|rcsb pdb:7L0S|rcsb pdb:7O7F|reactome:R-HSA-170670|reactome:R-HSA-392170|reactome:R-HSA-400042|reactome:R-HSA-418555|refseq:NP_002060.4|refseq:NP_001243343.1 ensembl:ENSG00000149295(gene)|ensembl:ENST00000362072(transcript)|ensembl:ENST00000542968(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001591"(dopamine neurotransmitter receptor activity, coupled via Gi/Go)|go:"GO:0001659"(temperature homeostasis)|go:"GO:0001666"(response to hypoxia)|go:"GO:0001669"(acrosomal vesicle)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0001975"(response to amphetamine)|go:"GO:0001976"(nervous system process involved in regulation of systemic arterial blood pressure)|go:"GO:0002027"(regulation of heart rate)|go:"GO:0002028"(regulation of sodium ion transport)|go:"GO:0002031"(G protein-coupled receptor internalization)|go:"GO:0002052"(positive regulation of neuroblast proliferation)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005929"(cilium)|go:"GO:0006874"(cellular calcium ion homeostasis)|go:"GO:0006914"(autophagy)|go:"GO:0007195"(adenylate cyclase-inhibiting dopamine receptor signaling pathway)|go:"GO:0007270"(neuron-neuron synaptic transmission)|go:"GO:0007409"(axonogenesis)|go:"GO:0007416"(synapse assembly)|go:"GO:0007608"(sensory perception of smell)|go:"GO:0007616"(long-term memory)|go:"GO:0007625"(grooming behavior)|go:"GO:0007626"(locomotory behavior)|go:"GO:0007628"(adult walking behavior)|go:"GO:0008104"(protein localization)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0008306"(associative learning)|go:"GO:0008542"(visual learning)|go:"GO:0009416"(response to light stimulus)|go:"GO:0009636"(response to toxic substance)|go:"GO:0010039"(response to iron ion)|go:"GO:0014059"(regulation of dopamine secretion)|go:"GO:0014069"(postsynaptic density)|go:"GO:0014854"(response to inactivity)|go:"GO:0015459"(potassium channel regulator activity)|go:"GO:0016055"(Wnt signaling pathway)|go:"GO:0016328"(lateral plasma membrane)|go:"GO:0021756"(striatum development)|go:"GO:0021769"(orbitofrontal cortex development)|go:"GO:0021853"(cerebral cortex GABAergic interneuron migration)|go:"GO:0021984"(adenohypophysis development)|go:"GO:0030139"(endocytic vesicle)|go:"GO:0030336"(negative regulation of cell migration)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0030432"(peristalsis)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0031223"(auditory behavior)|go:"GO:0032147"(activation of protein kinase activity)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032467"(positive regulation of cytokinesis)|go:"GO:0007194"(negative regulation of adenylate cyclase activity)|go:"GO:0032922"(circadian regulation of gene expression)|go:"GO:0034776"(response to histamine)|go:"GO:0035094"(response to nicotine)|go:"GO:0035240"(dopamine binding)|go:"GO:0035255"(ionotropic glutamate receptor binding)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0035810"(positive regulation of urine volume)|go:"GO:0035815"(positive regulation of renal sodium excretion)|go:"GO:0036126"(sperm flagellum)|go:"GO:0040018"(positive regulation of multicellular organism growth)|go:"GO:0042220"(response to cocaine)|go:"GO:0042321"(negative regulation of circadian sleep/wake cycle, sleep)|go:"GO:0042417"(dopamine metabolic process)|go:"GO:0042493"|go:"GO:0042756"(drinking behavior)|go:"GO:0042802"(identical protein binding)|go:"GO:0043197"(dendritic spine)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0043204"(perikaryon)|go:"GO:0043266"(regulation of potassium ion transport)|go:"GO:0043278"(response to morphine)|go:"GO:0043473"(pigmentation)|go:"GO:0043666"(regulation of phosphoprotein phosphatase activity)|go:"GO:0043679"(axon terminus)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045202"(synapse)|go:"GO:0045745"(positive regulation of G protein-coupled receptor signaling pathway)|go:"GO:0045824"(negative regulation of innate immune response)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046676"(negative regulation of insulin secretion)|go:"GO:0046717"(acid secretion)|go:"GO:0048148"(behavioral response to cocaine)|go:"GO:0048149"(behavioral response to ethanol)|go:"GO:0048169"(regulation of long-term neuronal synaptic plasticity)|go:"GO:0048678"(response to axon injury)|go:"GO:0048755"(branching morphogenesis of a nerve)|go:"GO:0050482"(arachidonic acid secretion)|go:"GO:0050709"(negative regulation of protein secretion)|go:"GO:0051209"(release of sequestered calcium ion into cytosol)|go:"GO:0051481"(negative regulation of cytosolic calcium ion concentration)|go:"GO:0051482"(positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway)|go:"GO:0051584"(regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0051586"(positive regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0051898"(negative regulation of protein kinase B signaling)|go:"GO:0051967"(negative regulation of synaptic transmission, glutamatergic)|go:"GO:0060079"(excitatory postsynaptic potential)|go:"GO:0060124"(positive regulation of growth hormone secretion)|go:"GO:0060134"(prepulse inhibition)|go:"GO:0060158"(phospholipase C-activating dopamine receptor signaling pathway)|go:"GO:0060160"(negative regulation of dopamine receptor signaling pathway)|go:"GO:0060170"(ciliary membrane)|go:"GO:0060548"(negative regulation of cell death)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071880"(adenylate cyclase-activating adrenergic receptor signaling pathway)|go:"GO:0090325"(regulation of locomotion involved in locomotory behavior)|go:"GO:0097730"(non-motile cilium)|go:"GO:0098691"(dopaminergic synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099055"(integral component of postsynaptic membrane)|go:"GO:0099056"(integral component of presynaptic membrane)|go:"GO:0099170"(postsynaptic modulation of chemical synaptic transmission)|go:"GO:1900168"(positive regulation of glial cell-derived neurotrophic factor production)|go:"GO:1900273"(positive regulation of long-term synaptic potentiation)|go:"GO:1901386"(negative regulation of voltage-gated calcium channel activity)|go:"GO:1990384"(hyaloid vascular plexus regression)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000929(Dopamine receptor)|interpro:IPR001922(Dopamine D2 receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|mint:P14416|rcsb pdb:1I15|rcsb pdb:5AER|rcsb pdb:6CM4|rcsb pdb:6LUQ|rcsb pdb:6VMS|rcsb pdb:7JVR|reactome:R-HSA-390651|go:"GO:0051823"(regulation of synapse structural plasticity)|refseq:NP_000786.1|refseq:NP_057658.2|refseq:XP_016872785.1|dip:DIP-5977N - - - figure legend:Supplementary Figure 5B|agonist:"Arachidonyl-2'-chloroethylamide (ACEA) - a selective, synthetic agonist of CB1R. In the presence of the blocking peptide agonist activity was decreased."|comment:Untagged CB1 was co-expressed.|agonist:"Quinpirole (D2L agonist). The presence of the blocking peptide did not affect the activity of Quinpirole."|comment:"The co-expression of CB1/D2L hetero-oligomer blocking peptide (CB1-BP aas 432-456), which inhibited the physical interaction between CB1 and D2 (Fig. 2C), inhibited the switch of CB1 and D2L coupling from Ga i to Gas proteins following co-activation of both receptors (Suppl. Fig. 5A,B)."|agonist:"ACEA plus Quinpirole (D2L agonist):-in the presence of CB1 blocking peptide the BRET signal increased."|caution:The figure legend and figure text give different interactors for this figure- the interactors from the figure legend have been assumed to be the correct ones.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:3yQOCXJb8Undp/Xw42xrBiG5ePE9606 rogid:S5beCpwOapTeUXcS45WlU5ApKEI9606 rigid:XgEiULzcTxKPQmBRGjsoC3lxdyI false renilla luciferase protein tag:93-94 green fluorescent protein tag:c-c - - psi-mi:"MI:0866"(tag visualisation) psi-mi:"MI:0866"(tag visualisation) uniprotkb:P29066 uniprotkb:P21554 intact:EBI-4303019|ensembl:ENSRNOP00000046069 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 psi-mi:arrb1_rat(display_long)|uniprotkb:Arrb1(gene name)|psi-mi:Arrb1(display_short)|uniprotkb:Arrestin beta-1(gene name synonym) psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:"MI:0012"(bioluminescence resonance energy transfer) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-21306817|imex:IM-26955-52 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:2334"(luminescence donor) psi-mi:"MI:2335"(luminescence acceptor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:2156|refseq:NP_037042.1|interpro:IPR011022(Arrestin-like, C-terminal)|interpro:IPR014752(Arrestin, C-terminal)|interpro:IPR014753(Arrestin, N-terminal)|interpro:IPR014756(Immunoglobulin E-set)|interpro:IPR017864|mint:P29066|rcsb pdb:4JQI|rcsb pdb:6KL7|rcsb pdb:6U1N|reactome:R-RNO-418555|reactome:R-RNO-432720|reactome:R-RNO-432722|reactome:R-RNO-456926|reactome:R-RNO-5635838|reactome:R-RNO-5674135|reactome:R-RNO-5689880|reactome:R-RNO-8856825|reactome:R-RNO-8856828|interpro:IPR011021(Arrestin-like, N-terminal)|ensembl:ENSRNOG00000030404(gene)|ensembl:ENSRNOT00000043554(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0000785"(chromatin)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0001934"(positive regulation of protein phosphorylation)|go:"GO:0002031"(G protein-coupled receptor internalization)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0003713"(transcription coactivator activity)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005159"(insulin-like growth factor receptor binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005768"(endosome)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0006897"(endocytosis)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007602"(phototransduction)|go:"GO:0008277"(regulation of G protein-coupled receptor signaling pathway)|go:"GO:0008284"(positive regulation of cell population proliferation)|go:"GO:0014069"(postsynaptic density)|go:"GO:0015031"(protein transport)|go:"GO:0016323"(basolateral plasma membrane)|go:"GO:0016567"(protein ubiquitination)|go:"GO:0016573"(histone acetylation)|go:"GO:0016604"(nuclear body)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0019899"(enzyme binding)|go:"GO:0030276"(clathrin binding)|go:"GO:0030331"(estrogen receptor binding)|go:"GO:0031143"(pseudopodium)|go:"GO:0031397"(negative regulation of protein ubiquitination)|go:"GO:0031398"(positive regulation of protein ubiquitination)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031434"(mitogen-activated protein kinase kinase binding)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0031691"(alpha-1A adrenergic receptor binding)|go:"GO:0031692"(alpha-1B adrenergic receptor binding)|go:"GO:0031701"(angiotensin receptor binding)|go:"GO:0031762"(follicle-stimulating hormone receptor binding)|go:"GO:0031896"(V2 vasopressin receptor binding)|go:"GO:0032088"(negative regulation of NF-kappaB transcription factor activity)|go:"GO:0032092"(positive regulation of protein binding)|go:"GO:0032715"(negative regulation of interleukin-6 production)|go:"GO:0032717"(negative regulation of interleukin-8 production)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|go:"GO:0034260"(negative regulation of GTPase activity)|go:"GO:0034393"(positive regulation of smooth muscle cell apoptotic process)|go:"GO:0035025"(positive regulation of Rho protein signal transduction)|go:"GO:0035066"(positive regulation of histone acetylation)|go:"GO:0035612"(AP-2 adaptor complex binding)|go:"GO:0035615"(clathrin adaptor activity)|go:"GO:0035774"(positive regulation of insulin secretion involved in cellular response to glucose stimulus)|go:"GO:0042493"|go:"GO:0042699"(follicle-stimulating hormone signaling pathway)|go:"GO:0042981"(regulation of apoptotic process)|go:"GO:0043027"(cysteine-type endopeptidase inhibitor activity involved in apoptotic process)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0043149"(stress fiber assembly)|go:"GO:0043154"(negative regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0043161"(proteasome-mediated ubiquitin-dependent protein catabolic process)|go:"GO:0043197"(dendritic spine)|go:"GO:0043280"(positive regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0043524"(negative regulation of neuron apoptotic process)|go:"GO:0044325"(transmembrane transporter binding)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0045309"(protein phosphorylated amino acid binding)|go:"GO:0045746"(negative regulation of Notch signaling pathway)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0050975"(sensory perception of touch)|go:"GO:0051219"(phosphoprotein binding)|go:"GO:0070373"(negative regulation of ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0090240"(positive regulation of histone H4 acetylation)|go:"GO:1990763"(arrestin family protein binding)|interpro:IPR000698(Arrestin)|dip:DIP-40808N go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N - - - figure legend:Suppl. Fig. 8A|agonist:"Arachidonyl-2'-chloroethylamide (ACEA) - a selective, synthetic agonist of CB1R."|comment:Untagged mGluR6 was co-expressed.|antagonist:"mGluR6 agonist L-2-amino-4-phosphonobutyric acid (L-AP4)"|comment:co-application of both ACEA and 1 L-AP4 resulted in similar BRET Eff signal between b-arrestin1-Rluc and CB1-GFP 2 as compared to cells treated with ACEA only.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:154Z++W8VsgmEd4rSCEVkw4xLgA10116 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rigid:6qKo3JcqNG6Ajzd0aaJlBBi8zFY false renilla luciferase protein tag:c-c green fluorescent protein tag:c-c - - psi-mi:"MI:0866"(tag visualisation) psi-mi:"MI:0866"(tag visualisation) uniprotkb:P29066 uniprotkb:P14416 intact:EBI-4303019|ensembl:ENSRNOP00000046069 intact:EBI-2928178|ensembl:ENSP00000354859|ensembl:ENSP00000442172|uniprotkb:Q9NZR3|uniprotkb:Q9UPA9 psi-mi:arrb1_rat(display_long)|uniprotkb:Arrb1(gene name)|psi-mi:Arrb1(display_short)|uniprotkb:Arrestin beta-1(gene name synonym) psi-mi:drd2_human(display_long)|uniprotkb:DRD2(gene name)|psi-mi:DRD2(display_short)|uniprotkb:Dopamine D2 receptor(gene name synonym) psi-mi:"MI:0012"(bioluminescence resonance energy transfer) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-21306944|imex:IM-26955-54 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:2335"(luminescence acceptor) psi-mi:"MI:2334"(luminescence donor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:2156|refseq:NP_037042.1|interpro:IPR011022(Arrestin-like, C-terminal)|interpro:IPR014752(Arrestin, C-terminal)|interpro:IPR014753(Arrestin, N-terminal)|interpro:IPR014756(Immunoglobulin E-set)|interpro:IPR017864|mint:P29066|rcsb pdb:4JQI|rcsb pdb:6KL7|rcsb pdb:6U1N|reactome:R-RNO-418555|reactome:R-RNO-432720|reactome:R-RNO-432722|reactome:R-RNO-456926|reactome:R-RNO-5635838|reactome:R-RNO-5674135|reactome:R-RNO-5689880|reactome:R-RNO-8856825|reactome:R-RNO-8856828|interpro:IPR011021(Arrestin-like, N-terminal)|ensembl:ENSRNOG00000030404(gene)|ensembl:ENSRNOT00000043554(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0000785"(chromatin)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0001934"(positive regulation of protein phosphorylation)|go:"GO:0002031"(G protein-coupled receptor internalization)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0003713"(transcription coactivator activity)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005159"(insulin-like growth factor receptor binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005768"(endosome)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0006897"(endocytosis)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007602"(phototransduction)|go:"GO:0008277"(regulation of G protein-coupled receptor signaling pathway)|go:"GO:0008284"(positive regulation of cell population proliferation)|go:"GO:0014069"(postsynaptic density)|go:"GO:0015031"(protein transport)|go:"GO:0016323"(basolateral plasma membrane)|go:"GO:0016567"(protein ubiquitination)|go:"GO:0016573"(histone acetylation)|go:"GO:0016604"(nuclear body)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0019899"(enzyme binding)|go:"GO:0030276"(clathrin binding)|go:"GO:0030331"(estrogen receptor binding)|go:"GO:0031143"(pseudopodium)|go:"GO:0031397"(negative regulation of protein ubiquitination)|go:"GO:0031398"(positive regulation of protein ubiquitination)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031434"(mitogen-activated protein kinase kinase binding)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0031691"(alpha-1A adrenergic receptor binding)|go:"GO:0031692"(alpha-1B adrenergic receptor binding)|go:"GO:0031701"(angiotensin receptor binding)|go:"GO:0031762"(follicle-stimulating hormone receptor binding)|go:"GO:0031896"(V2 vasopressin receptor binding)|go:"GO:0032088"(negative regulation of NF-kappaB transcription factor activity)|go:"GO:0032092"(positive regulation of protein binding)|go:"GO:0032715"(negative regulation of interleukin-6 production)|go:"GO:0032717"(negative regulation of interleukin-8 production)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|go:"GO:0034260"(negative regulation of GTPase activity)|go:"GO:0034393"(positive regulation of smooth muscle cell apoptotic process)|go:"GO:0035025"(positive regulation of Rho protein signal transduction)|go:"GO:0035066"(positive regulation of histone acetylation)|go:"GO:0035612"(AP-2 adaptor complex binding)|go:"GO:0035615"(clathrin adaptor activity)|go:"GO:0035774"(positive regulation of insulin secretion involved in cellular response to glucose stimulus)|go:"GO:0042493"|go:"GO:0042699"(follicle-stimulating hormone signaling pathway)|go:"GO:0042981"(regulation of apoptotic process)|go:"GO:0043027"(cysteine-type endopeptidase inhibitor activity involved in apoptotic process)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0043149"(stress fiber assembly)|go:"GO:0043154"(negative regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0043161"(proteasome-mediated ubiquitin-dependent protein catabolic process)|go:"GO:0043197"(dendritic spine)|go:"GO:0043280"(positive regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0043524"(negative regulation of neuron apoptotic process)|go:"GO:0044325"(transmembrane transporter binding)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0045309"(protein phosphorylated amino acid binding)|go:"GO:0045746"(negative regulation of Notch signaling pathway)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0050975"(sensory perception of touch)|go:"GO:0051219"(phosphoprotein binding)|go:"GO:0070373"(negative regulation of ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0090240"(positive regulation of histone H4 acetylation)|go:"GO:1990763"(arrestin family protein binding)|interpro:IPR000698(Arrestin)|dip:DIP-40808N ensembl:ENSG00000149295(gene)|ensembl:ENST00000362072(transcript)|ensembl:ENST00000542968(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001591"(dopamine neurotransmitter receptor activity, coupled via Gi/Go)|go:"GO:0001659"(temperature homeostasis)|go:"GO:0001666"(response to hypoxia)|go:"GO:0001669"(acrosomal vesicle)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0001975"(response to amphetamine)|go:"GO:0001976"(nervous system process involved in regulation of systemic arterial blood pressure)|go:"GO:0002027"(regulation of heart rate)|go:"GO:0002028"(regulation of sodium ion transport)|go:"GO:0002031"(G protein-coupled receptor internalization)|go:"GO:0002052"(positive regulation of neuroblast proliferation)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005929"(cilium)|go:"GO:0006874"(cellular calcium ion homeostasis)|go:"GO:0006914"(autophagy)|go:"GO:0007195"(adenylate cyclase-inhibiting dopamine receptor signaling pathway)|go:"GO:0007270"(neuron-neuron synaptic transmission)|go:"GO:0007409"(axonogenesis)|go:"GO:0007416"(synapse assembly)|go:"GO:0007608"(sensory perception of smell)|go:"GO:0007616"(long-term memory)|go:"GO:0007625"(grooming behavior)|go:"GO:0007626"(locomotory behavior)|go:"GO:0007628"(adult walking behavior)|go:"GO:0008104"(protein localization)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0008306"(associative learning)|go:"GO:0008542"(visual learning)|go:"GO:0009416"(response to light stimulus)|go:"GO:0009636"(response to toxic substance)|go:"GO:0010039"(response to iron ion)|go:"GO:0014059"(regulation of dopamine secretion)|go:"GO:0014069"(postsynaptic density)|go:"GO:0014854"(response to inactivity)|go:"GO:0015459"(potassium channel regulator activity)|go:"GO:0016055"(Wnt signaling pathway)|go:"GO:0016328"(lateral plasma membrane)|go:"GO:0021756"(striatum development)|go:"GO:0021769"(orbitofrontal cortex development)|go:"GO:0021853"(cerebral cortex GABAergic interneuron migration)|go:"GO:0021984"(adenohypophysis development)|go:"GO:0030139"(endocytic vesicle)|go:"GO:0030336"(negative regulation of cell migration)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0030432"(peristalsis)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0031223"(auditory behavior)|go:"GO:0032147"(activation of protein kinase activity)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032467"(positive regulation of cytokinesis)|go:"GO:0007194"(negative regulation of adenylate cyclase activity)|go:"GO:0032922"(circadian regulation of gene expression)|go:"GO:0034776"(response to histamine)|go:"GO:0035094"(response to nicotine)|go:"GO:0035240"(dopamine binding)|go:"GO:0035255"(ionotropic glutamate receptor binding)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0035810"(positive regulation of urine volume)|go:"GO:0035815"(positive regulation of renal sodium excretion)|go:"GO:0036126"(sperm flagellum)|go:"GO:0040018"(positive regulation of multicellular organism growth)|go:"GO:0042220"(response to cocaine)|go:"GO:0042321"(negative regulation of circadian sleep/wake cycle, sleep)|go:"GO:0042417"(dopamine metabolic process)|go:"GO:0042493"|go:"GO:0042756"(drinking behavior)|go:"GO:0042802"(identical protein binding)|go:"GO:0043197"(dendritic spine)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0043204"(perikaryon)|go:"GO:0043266"(regulation of potassium ion transport)|go:"GO:0043278"(response to morphine)|go:"GO:0043473"(pigmentation)|go:"GO:0043666"(regulation of phosphoprotein phosphatase activity)|go:"GO:0043679"(axon terminus)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045202"(synapse)|go:"GO:0045745"(positive regulation of G protein-coupled receptor signaling pathway)|go:"GO:0045824"(negative regulation of innate immune response)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046676"(negative regulation of insulin secretion)|go:"GO:0046717"(acid secretion)|go:"GO:0048148"(behavioral response to cocaine)|go:"GO:0048149"(behavioral response to ethanol)|go:"GO:0048169"(regulation of long-term neuronal synaptic plasticity)|go:"GO:0048678"(response to axon injury)|go:"GO:0048755"(branching morphogenesis of a nerve)|go:"GO:0050482"(arachidonic acid secretion)|go:"GO:0050709"(negative regulation of protein secretion)|go:"GO:0051209"(release of sequestered calcium ion into cytosol)|go:"GO:0051481"(negative regulation of cytosolic calcium ion concentration)|go:"GO:0051482"(positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway)|go:"GO:0051584"(regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0051586"(positive regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0051898"(negative regulation of protein kinase B signaling)|go:"GO:0051967"(negative regulation of synaptic transmission, glutamatergic)|go:"GO:0060079"(excitatory postsynaptic potential)|go:"GO:0060124"(positive regulation of growth hormone secretion)|go:"GO:0060134"(prepulse inhibition)|go:"GO:0060158"(phospholipase C-activating dopamine receptor signaling pathway)|go:"GO:0060160"(negative regulation of dopamine receptor signaling pathway)|go:"GO:0060170"(ciliary membrane)|go:"GO:0060548"(negative regulation of cell death)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071880"(adenylate cyclase-activating adrenergic receptor signaling pathway)|go:"GO:0090325"(regulation of locomotion involved in locomotory behavior)|go:"GO:0097730"(non-motile cilium)|go:"GO:0098691"(dopaminergic synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099055"(integral component of postsynaptic membrane)|go:"GO:0099056"(integral component of presynaptic membrane)|go:"GO:0099170"(postsynaptic modulation of chemical synaptic transmission)|go:"GO:1900168"(positive regulation of glial cell-derived neurotrophic factor production)|go:"GO:1900273"(positive regulation of long-term synaptic potentiation)|go:"GO:1901386"(negative regulation of voltage-gated calcium channel activity)|go:"GO:1990384"(hyaloid vascular plexus regression)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000929(Dopamine receptor)|interpro:IPR001922(Dopamine D2 receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|mint:P14416|rcsb pdb:1I15|rcsb pdb:5AER|rcsb pdb:6CM4|rcsb pdb:6LUQ|rcsb pdb:6VMS|rcsb pdb:7JVR|reactome:R-HSA-390651|go:"GO:0051823"(regulation of synapse structural plasticity)|refseq:NP_000786.1|refseq:NP_057658.2|refseq:XP_016872785.1|dip:DIP-5977N - - - figure legend:Supplementary figure 8B|comment:Untagged mGluR6 was co-expressed.|agonist:"Quinpirole (D2L agonist) concurrently with L-AP4 -an mGluR6 agonist, L-2-amino-4-phosphonobutyric acid."|comment:"The co-expression of un-tagged mGluR6 together with D 2L -Rluc and b-arrestin1-GFP 2 did not alter quinpirole-induced b-arrestin1 recruitment to D2L receptors in the absence or presence of the mGluR6 agonist (L-AP4)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:154Z++W8VsgmEd4rSCEVkw4xLgA10116 rogid:S5beCpwOapTeUXcS45WlU5ApKEI9606 rigid:O60Ve2WWzDCug8FG08mnb2VhXvE false green fluorescent protein tag:c-c renilla luciferase protein tag:c-c - - psi-mi:"MI:0866"(tag visualisation) psi-mi:"MI:0866"(tag visualisation) uniprotkb:P21554 uniprotkb:P21554 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:"MI:0051"(fluorescence technology) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-21300208|imex:IM-26955-23 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0584"(fluorescence acceptor) psi-mi:"MI:0584"(fluorescence acceptor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N - - - figure legend:Figure 2, Supplementary Figure 3|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rigid:BgN+WYynSK4bN6LGSe2Vb6xqtw4 false venus n-terminal fragment:c-c|ha tag:n-n venus c-terminal fragment:c-c|ha tag:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P21554 uniprotkb:P14416 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 intact:EBI-2928178|ensembl:ENSP00000354859|ensembl:ENSP00000442172|uniprotkb:Q9NZR3|uniprotkb:Q9UPA9 psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:drd2_human(display_long)|uniprotkb:DRD2(gene name)|psi-mi:DRD2(display_short)|uniprotkb:Dopamine D2 receptor(gene name synonym) psi-mi:"MI:0051"(fluorescence technology) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-21300208|imex:IM-26955-23 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0584"(fluorescence acceptor) psi-mi:"MI:2334"(luminescence donor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N ensembl:ENSG00000149295(gene)|ensembl:ENST00000362072(transcript)|ensembl:ENST00000542968(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001591"(dopamine neurotransmitter receptor activity, coupled via Gi/Go)|go:"GO:0001659"(temperature homeostasis)|go:"GO:0001666"(response to hypoxia)|go:"GO:0001669"(acrosomal vesicle)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0001975"(response to amphetamine)|go:"GO:0001976"(nervous system process involved in regulation of systemic arterial blood pressure)|go:"GO:0002027"(regulation of heart rate)|go:"GO:0002028"(regulation of sodium ion transport)|go:"GO:0002031"(G protein-coupled receptor internalization)|go:"GO:0002052"(positive regulation of neuroblast proliferation)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005929"(cilium)|go:"GO:0006874"(cellular calcium ion homeostasis)|go:"GO:0006914"(autophagy)|go:"GO:0007195"(adenylate cyclase-inhibiting dopamine receptor signaling pathway)|go:"GO:0007270"(neuron-neuron synaptic transmission)|go:"GO:0007409"(axonogenesis)|go:"GO:0007416"(synapse assembly)|go:"GO:0007608"(sensory perception of smell)|go:"GO:0007616"(long-term memory)|go:"GO:0007625"(grooming behavior)|go:"GO:0007626"(locomotory behavior)|go:"GO:0007628"(adult walking behavior)|go:"GO:0008104"(protein localization)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0008306"(associative learning)|go:"GO:0008542"(visual learning)|go:"GO:0009416"(response to light stimulus)|go:"GO:0009636"(response to toxic substance)|go:"GO:0010039"(response to iron ion)|go:"GO:0014059"(regulation of dopamine secretion)|go:"GO:0014069"(postsynaptic density)|go:"GO:0014854"(response to inactivity)|go:"GO:0015459"(potassium channel regulator activity)|go:"GO:0016055"(Wnt signaling pathway)|go:"GO:0016328"(lateral plasma membrane)|go:"GO:0021756"(striatum development)|go:"GO:0021769"(orbitofrontal cortex development)|go:"GO:0021853"(cerebral cortex GABAergic interneuron migration)|go:"GO:0021984"(adenohypophysis development)|go:"GO:0030139"(endocytic vesicle)|go:"GO:0030336"(negative regulation of cell migration)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0030432"(peristalsis)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0031223"(auditory behavior)|go:"GO:0032147"(activation of protein kinase activity)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032467"(positive regulation of cytokinesis)|go:"GO:0007194"(negative regulation of adenylate cyclase activity)|go:"GO:0032922"(circadian regulation of gene expression)|go:"GO:0034776"(response to histamine)|go:"GO:0035094"(response to nicotine)|go:"GO:0035240"(dopamine binding)|go:"GO:0035255"(ionotropic glutamate receptor binding)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0035810"(positive regulation of urine volume)|go:"GO:0035815"(positive regulation of renal sodium excretion)|go:"GO:0036126"(sperm flagellum)|go:"GO:0040018"(positive regulation of multicellular organism growth)|go:"GO:0042220"(response to cocaine)|go:"GO:0042321"(negative regulation of circadian sleep/wake cycle, sleep)|go:"GO:0042417"(dopamine metabolic process)|go:"GO:0042493"|go:"GO:0042756"(drinking behavior)|go:"GO:0042802"(identical protein binding)|go:"GO:0043197"(dendritic spine)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0043204"(perikaryon)|go:"GO:0043266"(regulation of potassium ion transport)|go:"GO:0043278"(response to morphine)|go:"GO:0043473"(pigmentation)|go:"GO:0043666"(regulation of phosphoprotein phosphatase activity)|go:"GO:0043679"(axon terminus)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045202"(synapse)|go:"GO:0045745"(positive regulation of G protein-coupled receptor signaling pathway)|go:"GO:0045824"(negative regulation of innate immune response)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046676"(negative regulation of insulin secretion)|go:"GO:0046717"(acid secretion)|go:"GO:0048148"(behavioral response to cocaine)|go:"GO:0048149"(behavioral response to ethanol)|go:"GO:0048169"(regulation of long-term neuronal synaptic plasticity)|go:"GO:0048678"(response to axon injury)|go:"GO:0048755"(branching morphogenesis of a nerve)|go:"GO:0050482"(arachidonic acid secretion)|go:"GO:0050709"(negative regulation of protein secretion)|go:"GO:0051209"(release of sequestered calcium ion into cytosol)|go:"GO:0051481"(negative regulation of cytosolic calcium ion concentration)|go:"GO:0051482"(positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway)|go:"GO:0051584"(regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0051586"(positive regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0051898"(negative regulation of protein kinase B signaling)|go:"GO:0051967"(negative regulation of synaptic transmission, glutamatergic)|go:"GO:0060079"(excitatory postsynaptic potential)|go:"GO:0060124"(positive regulation of growth hormone secretion)|go:"GO:0060134"(prepulse inhibition)|go:"GO:0060158"(phospholipase C-activating dopamine receptor signaling pathway)|go:"GO:0060160"(negative regulation of dopamine receptor signaling pathway)|go:"GO:0060170"(ciliary membrane)|go:"GO:0060548"(negative regulation of cell death)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071880"(adenylate cyclase-activating adrenergic receptor signaling pathway)|go:"GO:0090325"(regulation of locomotion involved in locomotory behavior)|go:"GO:0097730"(non-motile cilium)|go:"GO:0098691"(dopaminergic synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099055"(integral component of postsynaptic membrane)|go:"GO:0099056"(integral component of presynaptic membrane)|go:"GO:0099170"(postsynaptic modulation of chemical synaptic transmission)|go:"GO:1900168"(positive regulation of glial cell-derived neurotrophic factor production)|go:"GO:1900273"(positive regulation of long-term synaptic potentiation)|go:"GO:1901386"(negative regulation of voltage-gated calcium channel activity)|go:"GO:1990384"(hyaloid vascular plexus regression)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000929(Dopamine receptor)|interpro:IPR001922(Dopamine D2 receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|mint:P14416|rcsb pdb:1I15|rcsb pdb:5AER|rcsb pdb:6CM4|rcsb pdb:6LUQ|rcsb pdb:6VMS|rcsb pdb:7JVR|reactome:R-HSA-390651|go:"GO:0051823"(regulation of synapse structural plasticity)|refseq:NP_000786.1|refseq:NP_057658.2|refseq:XP_016872785.1|dip:DIP-5977N - - - figure legend:Figure 2, Supplementary Figure 3|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rogid:S5beCpwOapTeUXcS45WlU5ApKEI9606 rigid:BgN+WYynSK4bN6LGSe2Vb6xqtw4 false venus n-terminal fragment:c-c|ha tag:n-n renilla luciferase protein tag:c-c - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P21554 uniprotkb:P14416 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 intact:EBI-2928178|ensembl:ENSP00000354859|ensembl:ENSP00000442172|uniprotkb:Q9NZR3|uniprotkb:Q9UPA9 psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:drd2_human(display_long)|uniprotkb:DRD2(gene name)|psi-mi:DRD2(display_short)|uniprotkb:Dopamine D2 receptor(gene name synonym) psi-mi:"MI:0051"(fluorescence technology) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-21300208|imex:IM-26955-23 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0584"(fluorescence acceptor) psi-mi:"MI:2341"(luminescence transmitter) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N ensembl:ENSG00000149295(gene)|ensembl:ENST00000362072(transcript)|ensembl:ENST00000542968(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001591"(dopamine neurotransmitter receptor activity, coupled via Gi/Go)|go:"GO:0001659"(temperature homeostasis)|go:"GO:0001666"(response to hypoxia)|go:"GO:0001669"(acrosomal vesicle)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0001975"(response to amphetamine)|go:"GO:0001976"(nervous system process involved in regulation of systemic arterial blood pressure)|go:"GO:0002027"(regulation of heart rate)|go:"GO:0002028"(regulation of sodium ion transport)|go:"GO:0002031"(G protein-coupled receptor internalization)|go:"GO:0002052"(positive regulation of neuroblast proliferation)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005929"(cilium)|go:"GO:0006874"(cellular calcium ion homeostasis)|go:"GO:0006914"(autophagy)|go:"GO:0007195"(adenylate cyclase-inhibiting dopamine receptor signaling pathway)|go:"GO:0007270"(neuron-neuron synaptic transmission)|go:"GO:0007409"(axonogenesis)|go:"GO:0007416"(synapse assembly)|go:"GO:0007608"(sensory perception of smell)|go:"GO:0007616"(long-term memory)|go:"GO:0007625"(grooming behavior)|go:"GO:0007626"(locomotory behavior)|go:"GO:0007628"(adult walking behavior)|go:"GO:0008104"(protein localization)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0008306"(associative learning)|go:"GO:0008542"(visual learning)|go:"GO:0009416"(response to light stimulus)|go:"GO:0009636"(response to toxic substance)|go:"GO:0010039"(response to iron ion)|go:"GO:0014059"(regulation of dopamine secretion)|go:"GO:0014069"(postsynaptic density)|go:"GO:0014854"(response to inactivity)|go:"GO:0015459"(potassium channel regulator activity)|go:"GO:0016055"(Wnt signaling pathway)|go:"GO:0016328"(lateral plasma membrane)|go:"GO:0021756"(striatum development)|go:"GO:0021769"(orbitofrontal cortex development)|go:"GO:0021853"(cerebral cortex GABAergic interneuron migration)|go:"GO:0021984"(adenohypophysis development)|go:"GO:0030139"(endocytic vesicle)|go:"GO:0030336"(negative regulation of cell migration)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0030432"(peristalsis)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0031223"(auditory behavior)|go:"GO:0032147"(activation of protein kinase activity)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032467"(positive regulation of cytokinesis)|go:"GO:0007194"(negative regulation of adenylate cyclase activity)|go:"GO:0032922"(circadian regulation of gene expression)|go:"GO:0034776"(response to histamine)|go:"GO:0035094"(response to nicotine)|go:"GO:0035240"(dopamine binding)|go:"GO:0035255"(ionotropic glutamate receptor binding)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0035810"(positive regulation of urine volume)|go:"GO:0035815"(positive regulation of renal sodium excretion)|go:"GO:0036126"(sperm flagellum)|go:"GO:0040018"(positive regulation of multicellular organism growth)|go:"GO:0042220"(response to cocaine)|go:"GO:0042321"(negative regulation of circadian sleep/wake cycle, sleep)|go:"GO:0042417"(dopamine metabolic process)|go:"GO:0042493"|go:"GO:0042756"(drinking behavior)|go:"GO:0042802"(identical protein binding)|go:"GO:0043197"(dendritic spine)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0043204"(perikaryon)|go:"GO:0043266"(regulation of potassium ion transport)|go:"GO:0043278"(response to morphine)|go:"GO:0043473"(pigmentation)|go:"GO:0043666"(regulation of phosphoprotein phosphatase activity)|go:"GO:0043679"(axon terminus)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045202"(synapse)|go:"GO:0045745"(positive regulation of G protein-coupled receptor signaling pathway)|go:"GO:0045824"(negative regulation of innate immune response)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046676"(negative regulation of insulin secretion)|go:"GO:0046717"(acid secretion)|go:"GO:0048148"(behavioral response to cocaine)|go:"GO:0048149"(behavioral response to ethanol)|go:"GO:0048169"(regulation of long-term neuronal synaptic plasticity)|go:"GO:0048678"(response to axon injury)|go:"GO:0048755"(branching morphogenesis of a nerve)|go:"GO:0050482"(arachidonic acid secretion)|go:"GO:0050709"(negative regulation of protein secretion)|go:"GO:0051209"(release of sequestered calcium ion into cytosol)|go:"GO:0051481"(negative regulation of cytosolic calcium ion concentration)|go:"GO:0051482"(positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway)|go:"GO:0051584"(regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0051586"(positive regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0051898"(negative regulation of protein kinase B signaling)|go:"GO:0051967"(negative regulation of synaptic transmission, glutamatergic)|go:"GO:0060079"(excitatory postsynaptic potential)|go:"GO:0060124"(positive regulation of growth hormone secretion)|go:"GO:0060134"(prepulse inhibition)|go:"GO:0060158"(phospholipase C-activating dopamine receptor signaling pathway)|go:"GO:0060160"(negative regulation of dopamine receptor signaling pathway)|go:"GO:0060170"(ciliary membrane)|go:"GO:0060548"(negative regulation of cell death)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071880"(adenylate cyclase-activating adrenergic receptor signaling pathway)|go:"GO:0090325"(regulation of locomotion involved in locomotory behavior)|go:"GO:0097730"(non-motile cilium)|go:"GO:0098691"(dopaminergic synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099055"(integral component of postsynaptic membrane)|go:"GO:0099056"(integral component of presynaptic membrane)|go:"GO:0099170"(postsynaptic modulation of chemical synaptic transmission)|go:"GO:1900168"(positive regulation of glial cell-derived neurotrophic factor production)|go:"GO:1900273"(positive regulation of long-term synaptic potentiation)|go:"GO:1901386"(negative regulation of voltage-gated calcium channel activity)|go:"GO:1990384"(hyaloid vascular plexus regression)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000929(Dopamine receptor)|interpro:IPR001922(Dopamine D2 receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|mint:P14416|rcsb pdb:1I15|rcsb pdb:5AER|rcsb pdb:6CM4|rcsb pdb:6LUQ|rcsb pdb:6VMS|rcsb pdb:7JVR|reactome:R-HSA-390651|go:"GO:0051823"(regulation of synapse structural plasticity)|refseq:NP_000786.1|refseq:NP_057658.2|refseq:XP_016872785.1|dip:DIP-5977N - - - figure legend:Figure 2, Supplementary Figure 3|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rogid:S5beCpwOapTeUXcS45WlU5ApKEI9606 rigid:BgN+WYynSK4bN6LGSe2Vb6xqtw4 false venus n-terminal fragment:c-c|ha tag:n-n green fluorescent protein tag:c-c - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P21554 uniprotkb:P21554 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:"MI:0051"(fluorescence technology) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-21300228|imex:IM-26955-25 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0941"(competitor) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0584"(fluorescence acceptor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N - - - figure legend:Figure 2C, Supplementary Figure 3|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rigid:BgN+WYynSK4bN6LGSe2Vb6xqtw4 false sufficient binding region:432-456 venus n-terminal fragment:c-c|ha tag:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P21554 uniprotkb:P21554 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:"MI:0051"(fluorescence technology) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-21300228|imex:IM-26955-25 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0941"(competitor) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0584"(fluorescence acceptor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N - - - figure legend:Figure 2C, Supplementary Figure 3|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rigid:BgN+WYynSK4bN6LGSe2Vb6xqtw4 false sufficient binding region:432-456 venus c-terminal fragment:c-c|ha tag:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P21554 uniprotkb:P14416 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 intact:EBI-2928178|ensembl:ENSP00000354859|ensembl:ENSP00000442172|uniprotkb:Q9NZR3|uniprotkb:Q9UPA9 psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:drd2_human(display_long)|uniprotkb:DRD2(gene name)|psi-mi:DRD2(display_short)|uniprotkb:Dopamine D2 receptor(gene name synonym) psi-mi:"MI:0051"(fluorescence technology) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-21300228|imex:IM-26955-25 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0941"(competitor) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:2334"(luminescence donor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N ensembl:ENSG00000149295(gene)|ensembl:ENST00000362072(transcript)|ensembl:ENST00000542968(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001591"(dopamine neurotransmitter receptor activity, coupled via Gi/Go)|go:"GO:0001659"(temperature homeostasis)|go:"GO:0001666"(response to hypoxia)|go:"GO:0001669"(acrosomal vesicle)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0001975"(response to amphetamine)|go:"GO:0001976"(nervous system process involved in regulation of systemic arterial blood pressure)|go:"GO:0002027"(regulation of heart rate)|go:"GO:0002028"(regulation of sodium ion transport)|go:"GO:0002031"(G protein-coupled receptor internalization)|go:"GO:0002052"(positive regulation of neuroblast proliferation)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005929"(cilium)|go:"GO:0006874"(cellular calcium ion homeostasis)|go:"GO:0006914"(autophagy)|go:"GO:0007195"(adenylate cyclase-inhibiting dopamine receptor signaling pathway)|go:"GO:0007270"(neuron-neuron synaptic transmission)|go:"GO:0007409"(axonogenesis)|go:"GO:0007416"(synapse assembly)|go:"GO:0007608"(sensory perception of smell)|go:"GO:0007616"(long-term memory)|go:"GO:0007625"(grooming behavior)|go:"GO:0007626"(locomotory behavior)|go:"GO:0007628"(adult walking behavior)|go:"GO:0008104"(protein localization)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0008306"(associative learning)|go:"GO:0008542"(visual learning)|go:"GO:0009416"(response to light stimulus)|go:"GO:0009636"(response to toxic substance)|go:"GO:0010039"(response to iron ion)|go:"GO:0014059"(regulation of dopamine secretion)|go:"GO:0014069"(postsynaptic density)|go:"GO:0014854"(response to inactivity)|go:"GO:0015459"(potassium channel regulator activity)|go:"GO:0016055"(Wnt signaling pathway)|go:"GO:0016328"(lateral plasma membrane)|go:"GO:0021756"(striatum development)|go:"GO:0021769"(orbitofrontal cortex development)|go:"GO:0021853"(cerebral cortex GABAergic interneuron migration)|go:"GO:0021984"(adenohypophysis development)|go:"GO:0030139"(endocytic vesicle)|go:"GO:0030336"(negative regulation of cell migration)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0030432"(peristalsis)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0031223"(auditory behavior)|go:"GO:0032147"(activation of protein kinase activity)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032467"(positive regulation of cytokinesis)|go:"GO:0007194"(negative regulation of adenylate cyclase activity)|go:"GO:0032922"(circadian regulation of gene expression)|go:"GO:0034776"(response to histamine)|go:"GO:0035094"(response to nicotine)|go:"GO:0035240"(dopamine binding)|go:"GO:0035255"(ionotropic glutamate receptor binding)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0035810"(positive regulation of urine volume)|go:"GO:0035815"(positive regulation of renal sodium excretion)|go:"GO:0036126"(sperm flagellum)|go:"GO:0040018"(positive regulation of multicellular organism growth)|go:"GO:0042220"(response to cocaine)|go:"GO:0042321"(negative regulation of circadian sleep/wake cycle, sleep)|go:"GO:0042417"(dopamine metabolic process)|go:"GO:0042493"|go:"GO:0042756"(drinking behavior)|go:"GO:0042802"(identical protein binding)|go:"GO:0043197"(dendritic spine)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0043204"(perikaryon)|go:"GO:0043266"(regulation of potassium ion transport)|go:"GO:0043278"(response to morphine)|go:"GO:0043473"(pigmentation)|go:"GO:0043666"(regulation of phosphoprotein phosphatase activity)|go:"GO:0043679"(axon terminus)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045202"(synapse)|go:"GO:0045745"(positive regulation of G protein-coupled receptor signaling pathway)|go:"GO:0045824"(negative regulation of innate immune response)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046676"(negative regulation of insulin secretion)|go:"GO:0046717"(acid secretion)|go:"GO:0048148"(behavioral response to cocaine)|go:"GO:0048149"(behavioral response to ethanol)|go:"GO:0048169"(regulation of long-term neuronal synaptic plasticity)|go:"GO:0048678"(response to axon injury)|go:"GO:0048755"(branching morphogenesis of a nerve)|go:"GO:0050482"(arachidonic acid secretion)|go:"GO:0050709"(negative regulation of protein secretion)|go:"GO:0051209"(release of sequestered calcium ion into cytosol)|go:"GO:0051481"(negative regulation of cytosolic calcium ion concentration)|go:"GO:0051482"(positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway)|go:"GO:0051584"(regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0051586"(positive regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0051898"(negative regulation of protein kinase B signaling)|go:"GO:0051967"(negative regulation of synaptic transmission, glutamatergic)|go:"GO:0060079"(excitatory postsynaptic potential)|go:"GO:0060124"(positive regulation of growth hormone secretion)|go:"GO:0060134"(prepulse inhibition)|go:"GO:0060158"(phospholipase C-activating dopamine receptor signaling pathway)|go:"GO:0060160"(negative regulation of dopamine receptor signaling pathway)|go:"GO:0060170"(ciliary membrane)|go:"GO:0060548"(negative regulation of cell death)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071880"(adenylate cyclase-activating adrenergic receptor signaling pathway)|go:"GO:0090325"(regulation of locomotion involved in locomotory behavior)|go:"GO:0097730"(non-motile cilium)|go:"GO:0098691"(dopaminergic synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099055"(integral component of postsynaptic membrane)|go:"GO:0099056"(integral component of presynaptic membrane)|go:"GO:0099170"(postsynaptic modulation of chemical synaptic transmission)|go:"GO:1900168"(positive regulation of glial cell-derived neurotrophic factor production)|go:"GO:1900273"(positive regulation of long-term synaptic potentiation)|go:"GO:1901386"(negative regulation of voltage-gated calcium channel activity)|go:"GO:1990384"(hyaloid vascular plexus regression)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000929(Dopamine receptor)|interpro:IPR001922(Dopamine D2 receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|mint:P14416|rcsb pdb:1I15|rcsb pdb:5AER|rcsb pdb:6CM4|rcsb pdb:6LUQ|rcsb pdb:6VMS|rcsb pdb:7JVR|reactome:R-HSA-390651|go:"GO:0051823"(regulation of synapse structural plasticity)|refseq:NP_000786.1|refseq:NP_057658.2|refseq:XP_016872785.1|dip:DIP-5977N - - - figure legend:Figure 2C, Supplementary Figure 3|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rogid:S5beCpwOapTeUXcS45WlU5ApKEI9606 rigid:BgN+WYynSK4bN6LGSe2Vb6xqtw4 false sufficient binding region:432-456 renilla luciferase protein tag:c-c - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P21554 uniprotkb:P14416 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 intact:EBI-2928178|ensembl:ENSP00000354859|ensembl:ENSP00000442172|uniprotkb:Q9NZR3|uniprotkb:Q9UPA9 psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:drd2_human(display_long)|uniprotkb:DRD2(gene name)|psi-mi:DRD2(display_short)|uniprotkb:Dopamine D2 receptor(gene name synonym) psi-mi:"MI:0051"(fluorescence technology) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-21300228|imex:IM-26955-25 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0941"(competitor) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:2341"(luminescence transmitter) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N ensembl:ENSG00000149295(gene)|ensembl:ENST00000362072(transcript)|ensembl:ENST00000542968(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001591"(dopamine neurotransmitter receptor activity, coupled via Gi/Go)|go:"GO:0001659"(temperature homeostasis)|go:"GO:0001666"(response to hypoxia)|go:"GO:0001669"(acrosomal vesicle)|go:"GO:0001933"(negative regulation of protein phosphorylation)|go:"GO:0001975"(response to amphetamine)|go:"GO:0001976"(nervous system process involved in regulation of systemic arterial blood pressure)|go:"GO:0002027"(regulation of heart rate)|go:"GO:0002028"(regulation of sodium ion transport)|go:"GO:0002031"(G protein-coupled receptor internalization)|go:"GO:0002052"(positive regulation of neuroblast proliferation)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005929"(cilium)|go:"GO:0006874"(cellular calcium ion homeostasis)|go:"GO:0006914"(autophagy)|go:"GO:0007195"(adenylate cyclase-inhibiting dopamine receptor signaling pathway)|go:"GO:0007270"(neuron-neuron synaptic transmission)|go:"GO:0007409"(axonogenesis)|go:"GO:0007416"(synapse assembly)|go:"GO:0007608"(sensory perception of smell)|go:"GO:0007616"(long-term memory)|go:"GO:0007625"(grooming behavior)|go:"GO:0007626"(locomotory behavior)|go:"GO:0007628"(adult walking behavior)|go:"GO:0008104"(protein localization)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0008306"(associative learning)|go:"GO:0008542"(visual learning)|go:"GO:0009416"(response to light stimulus)|go:"GO:0009636"(response to toxic substance)|go:"GO:0010039"(response to iron ion)|go:"GO:0014059"(regulation of dopamine secretion)|go:"GO:0014069"(postsynaptic density)|go:"GO:0014854"(response to inactivity)|go:"GO:0015459"(potassium channel regulator activity)|go:"GO:0016055"(Wnt signaling pathway)|go:"GO:0016328"(lateral plasma membrane)|go:"GO:0021756"(striatum development)|go:"GO:0021769"(orbitofrontal cortex development)|go:"GO:0021853"(cerebral cortex GABAergic interneuron migration)|go:"GO:0021984"(adenohypophysis development)|go:"GO:0030139"(endocytic vesicle)|go:"GO:0030336"(negative regulation of cell migration)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0030432"(peristalsis)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0031223"(auditory behavior)|go:"GO:0032147"(activation of protein kinase activity)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032467"(positive regulation of cytokinesis)|go:"GO:0007194"(negative regulation of adenylate cyclase activity)|go:"GO:0032922"(circadian regulation of gene expression)|go:"GO:0034776"(response to histamine)|go:"GO:0035094"(response to nicotine)|go:"GO:0035240"(dopamine binding)|go:"GO:0035255"(ionotropic glutamate receptor binding)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0035810"(positive regulation of urine volume)|go:"GO:0035815"(positive regulation of renal sodium excretion)|go:"GO:0036126"(sperm flagellum)|go:"GO:0040018"(positive regulation of multicellular organism growth)|go:"GO:0042220"(response to cocaine)|go:"GO:0042321"(negative regulation of circadian sleep/wake cycle, sleep)|go:"GO:0042417"(dopamine metabolic process)|go:"GO:0042493"|go:"GO:0042756"(drinking behavior)|go:"GO:0042802"(identical protein binding)|go:"GO:0043197"(dendritic spine)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0043204"(perikaryon)|go:"GO:0043266"(regulation of potassium ion transport)|go:"GO:0043278"(response to morphine)|go:"GO:0043473"(pigmentation)|go:"GO:0043666"(regulation of phosphoprotein phosphatase activity)|go:"GO:0043679"(axon terminus)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045202"(synapse)|go:"GO:0045745"(positive regulation of G protein-coupled receptor signaling pathway)|go:"GO:0045824"(negative regulation of innate immune response)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046676"(negative regulation of insulin secretion)|go:"GO:0046717"(acid secretion)|go:"GO:0048148"(behavioral response to cocaine)|go:"GO:0048149"(behavioral response to ethanol)|go:"GO:0048169"(regulation of long-term neuronal synaptic plasticity)|go:"GO:0048678"(response to axon injury)|go:"GO:0048755"(branching morphogenesis of a nerve)|go:"GO:0050482"(arachidonic acid secretion)|go:"GO:0050709"(negative regulation of protein secretion)|go:"GO:0051209"(release of sequestered calcium ion into cytosol)|go:"GO:0051481"(negative regulation of cytosolic calcium ion concentration)|go:"GO:0051482"(positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway)|go:"GO:0051584"(regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0051586"(positive regulation of dopamine uptake involved in synaptic transmission)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0051898"(negative regulation of protein kinase B signaling)|go:"GO:0051967"(negative regulation of synaptic transmission, glutamatergic)|go:"GO:0060079"(excitatory postsynaptic potential)|go:"GO:0060124"(positive regulation of growth hormone secretion)|go:"GO:0060134"(prepulse inhibition)|go:"GO:0060158"(phospholipase C-activating dopamine receptor signaling pathway)|go:"GO:0060160"(negative regulation of dopamine receptor signaling pathway)|go:"GO:0060170"(ciliary membrane)|go:"GO:0060548"(negative regulation of cell death)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071880"(adenylate cyclase-activating adrenergic receptor signaling pathway)|go:"GO:0090325"(regulation of locomotion involved in locomotory behavior)|go:"GO:0097730"(non-motile cilium)|go:"GO:0098691"(dopaminergic synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099055"(integral component of postsynaptic membrane)|go:"GO:0099056"(integral component of presynaptic membrane)|go:"GO:0099170"(postsynaptic modulation of chemical synaptic transmission)|go:"GO:1900168"(positive regulation of glial cell-derived neurotrophic factor production)|go:"GO:1900273"(positive regulation of long-term synaptic potentiation)|go:"GO:1901386"(negative regulation of voltage-gated calcium channel activity)|go:"GO:1990384"(hyaloid vascular plexus regression)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000929(Dopamine receptor)|interpro:IPR001922(Dopamine D2 receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|mint:P14416|rcsb pdb:1I15|rcsb pdb:5AER|rcsb pdb:6CM4|rcsb pdb:6LUQ|rcsb pdb:6VMS|rcsb pdb:7JVR|reactome:R-HSA-390651|go:"GO:0051823"(regulation of synapse structural plasticity)|refseq:NP_000786.1|refseq:NP_057658.2|refseq:XP_016872785.1|dip:DIP-5977N - - - figure legend:Figure 2C, Supplementary Figure 3|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293a)|taxid:9606(Homo sapiens) - 2019/04/03 2019/07/04 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rogid:S5beCpwOapTeUXcS45WlU5ApKEI9606 rigid:BgN+WYynSK4bN6LGSe2Vb6xqtw4 false sufficient binding region:432-456 green fluorescent protein tag:c-c - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P63096 uniprotkb:P21554 intact:EBI-618639|ensembl:ENSP00000497051|ensembl:ENSP00000497179|ensembl:ENSP00000497260|ensembl:ENSP00000497315|ensembl:ENSP00000497379|ensembl:ENSP00000497717|ensembl:ENSP00000497754|ensembl:ENSP00000497760|ensembl:ENSP00000497765|ensembl:ENSP00000497773|ensembl:ENSP00000498091|ensembl:ENSP00000343027|ensembl:ENSP00000391439|ensembl:ENSP00000496800|ensembl:ENSP00000496829|uniprotkb:P04898|uniprotkb:P11015|uniprotkb:P31871|uniprotkb:Q5U074|uniprotkb:Q8TAN5|uniprotkb:Q9UGA4|uniprotkb:A8KA88|uniprotkb:B4E2V1|uniprotkb:C9J3A4 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 psi-mi:gnai1_human(display_long)|uniprotkb:GNAI1(gene name)|psi-mi:GNAI1(display_short)|uniprotkb:Adenylate cyclase-inhibiting G alpha protein(gene name synonym) psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:"MI:0012"(bioluminescence resonance energy transfer) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-21306696|imex:IM-26955-48 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:2334"(luminescence donor) psi-mi:"MI:2335"(luminescence acceptor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) reactome:R-HSA-418597|reactome:R-HSA-422356|reactome:R-HSA-9009391|reactome:R-HSA-9660821|reactome:R-HSA-418594|ensembl:ENST00000351004(transcript)|ensembl:ENST00000442586(transcript)|ensembl:ENST00000648098(transcript)|ensembl:ENSG00000127955(gene)|ensembl:ENST00000648306(transcript)|ensembl:ENST00000648412(transcript)|ensembl:ENST00000648476(transcript)|ensembl:ENST00000648663(transcript)|ensembl:ENST00000648832(transcript)|ensembl:ENST00000648877(transcript)|ensembl:ENST00000648953(transcript)|ensembl:ENST00000649225(transcript)|ensembl:ENST00000649267(transcript)|ensembl:ENST00000649487(transcript)|ensembl:ENST00000649796(transcript)|ensembl:ENST00000649855(transcript)|go:"GO:0000287"(magnesium ion binding)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005765"(lysosomal membrane)|go:"GO:0005813"(centrosome)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0007049"(cell cycle)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007193"(adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway)|go:"GO:0019003"(GDP binding)|go:"GO:0030496"(midbody)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0031821"(G protein-coupled serotonin receptor binding)|go:"GO:0032794"(GTPase activating protein binding)|go:"GO:0043434"(response to peptide hormone)|go:"GO:0043949"(regulation of cAMP-mediated signaling)|go:"GO:0045121"(membrane raft)|go:"GO:0050805"(negative regulation of synaptic transmission)|go:"GO:0051301"(cell division)|go:"GO:0060236"(regulation of mitotic spindle organization)|go:"GO:0070062"(extracellular exosome)|go:"GO:0099738"(cell cortex region)|go:"GO:1904322"(cellular response to forskolin)|go:"GO:1904778"(positive regulation of protein localization to cell cortex)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR001408(G-protein alpha subunit, group I)|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|mint:P63096|rcsb pdb:1KJY|rcsb pdb:1Y3A|rcsb pdb:2G83|rcsb pdb:2GTP|rcsb pdb:2IK8|rcsb pdb:2OM2|rcsb pdb:2XNS|rcsb pdb:3ONW|rcsb pdb:3QE0|rcsb pdb:3QI2|rcsb pdb:3UMR|rcsb pdb:3UMS|rcsb pdb:4G5Q|rcsb pdb:5JS7|rcsb pdb:5JS8|rcsb pdb:5TDH|rcsb pdb:6CMO|rcsb pdb:6CRK|rcsb pdb:6DDE|rcsb pdb:6DDF|rcsb pdb:6KPF|rcsb pdb:6KPG|rcsb pdb:6LFM|rcsb pdb:6LFO|rcsb pdb:6LML|rcsb pdb:6N4B|rcsb pdb:6OMM|rcsb pdb:6OS9|rcsb pdb:6OSA|rcsb pdb:6OT0|rcsb pdb:6PB0|rcsb pdb:6PB1|rcsb pdb:6PT0|rcsb pdb:6QNO|rcsb pdb:6VU8|rcsb pdb:6XBJ|rcsb pdb:6XBK|rcsb pdb:6XBL|rcsb pdb:6XBM|rcsb pdb:7CMU|rcsb pdb:7CMV|rcsb pdb:7E2X|rcsb pdb:7E2Y|rcsb pdb:7E2Z|rcsb pdb:7E32|rcsb pdb:7E33|rcsb pdb:7E9G|rcsb pdb:7EB2|rcsb pdb:7JVR|rcsb pdb:7L0P|rcsb pdb:7L0Q|rcsb pdb:7L0R|rcsb pdb:7L0S|rcsb pdb:7O7F|reactome:R-HSA-170670|reactome:R-HSA-392170|reactome:R-HSA-400042|reactome:R-HSA-418555|refseq:NP_002060.4|refseq:NP_001243343.1 go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N - - - figure legend:Supplementary Fig 7A|agonist:"Arachidonyl-2'-chloroethylamide (ACEA) - a selective, synthetic agonist of CB1R."|antagonist:"Quinpirole (D2L agonist) added concurrently with Arachidonyl-2'-chloroethylamide (ACEA) (a selective, synthetic agonist of CB1R), but only when untagged D2L was co-expressed. This effect was reduced by addition of O-2050 -a small molecule cannabinoid derivative, acting as an antagonist for the CB1 receptor."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:11090(mouse-STHdhQ7/Q7)|taxid:11090(mouse STHdhQ7/Q7) - 2019/04/03 2019/07/04 rogid:3yQOCXJb8Undp/Xw42xrBiG5ePE9606 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rigid:IzLXYnd5auXbNl36x+xrzzb18Bw false renilla luciferase protein tag:93-94 green fluorescent protein tag:c-c - - psi-mi:"MI:0866"(tag visualisation) psi-mi:"MI:0866"(tag visualisation) uniprotkb:P63092-1 uniprotkb:P21554 intact:EBI-11428973|uniprotkb:P04895-1|ensembl:ENSP00000360126 intact:EBI-2909859|ensembl:ENSP00000358511|ensembl:ENSP00000358513|ensembl:ENSP00000412192|ensembl:ENSP00000446702|ensembl:ENSP00000446819|uniprotkb:B2R9T4|uniprotkb:Q13949|uniprotkb:Q495Z0|uniprotkb:Q4PLI4|uniprotkb:Q4VBM6|uniprotkb:Q5JVL5|uniprotkb:Q5UB37|uniprotkb:Q9UNN0|uniprotkb:E1P512 psi-mi:p63092-1(display_long)|uniprotkb:Adenylate cyclase-stimulating G alpha protein(gene name synonym)|uniprotkb:GNAS(gene name)|psi-mi:GNAS(display_short)|uniprotkb:GNAS1(gene name synonym)|uniprotkb:GSP(gene name synonym)|uniprotkb:Alpha-S2(isoform synonym)|uniprotkb:GNASl(isoform synonym)|uniprotkb:Alpha-S-long(isoform synonym) psi-mi:cnr1_human(display_long)|uniprotkb:CNR1(gene name)|psi-mi:CNR1(display_short)|uniprotkb:CNR(gene name synonym)|uniprotkb:CANN6(gene name synonym) psi-mi:"MI:0012"(bioluminescence resonance energy transfer) Bagher et al. (2017) pubmed:28734930|imex:IM-26955 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-21306743|imex:IM-26955-50 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:2334"(luminescence donor) psi-mi:"MI:2335"(luminescence acceptor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-1047114(isoform-parent)|ensembl:ENSG00000087460(gene)|ensembl:ENST00000371085(transcript) go:"GO:0099635"(voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000810(Cannabinoid receptor, type 1)|interpro:IPR002230(Cannabinoid receptor)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1LVQ|rcsb pdb:1LVR|rcsb pdb:2B0Y|rcsb pdb:2KOE|rcsb pdb:2MZ2|rcsb pdb:2MZ3|rcsb pdb:2MZA|rcsb pdb:5TGZ|rcsb pdb:5U09|rcsb pdb:5XR8|rcsb pdb:5XRA|rcsb pdb:6KPG|rcsb pdb:6KQI|rcsb pdb:6N4B|reactome:R-HSA-373076|reactome:R-HSA-418594|go:"GO:0099553"(trans-synaptic signaling by endocannabinoid, modulating synaptic transmission)|go:"GO:0007584"(response to nutrient)|go:"GO:0007613"(memory)|go:"GO:0010976"(positive regulation of neuron projection development)|go:"GO:0014063"(negative regulation of serotonin secretion)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019222"(regulation of metabolic process)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030426"(growth cone)|go:"GO:0031622"(positive regulation of fever generation)|go:"GO:0031999"(negative regulation of fatty acid beta-oxidation)|ensembl:ENSG00000118432(gene)|ensembl:ENST00000369499(transcript)|ensembl:ENST00000369501(transcript)|ensembl:ENST00000428600(transcript)|ensembl:ENST00000549890(transcript)|ensembl:ENST00000551417(transcript)|go:"GO:0002866"(positive regulation of acute inflammatory response to antigenic stimulus)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0004949"(cannabinoid receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007413"(axonal fasciculation)|go:"GO:0007568"(aging)|go:"GO:0032228"(regulation of synaptic transmission, GABAergic)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0033004"(negative regulation of mast cell activation)|go:"GO:0033602"(negative regulation of dopamine secretion)|go:"GO:0035094"(response to nicotine)|go:"GO:0038171"(cannabinoid signaling pathway)|go:"GO:0042220"(response to cocaine)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042802"(identical protein binding)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043278"(response to morphine)|go:"GO:0045121"(membrane raft)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0045776"(negative regulation of blood pressure)|go:"GO:0045777"(positive regulation of blood pressure)|go:"GO:0050796"(regulation of insulin secretion)|go:"GO:0051001"(negative regulation of nitric-oxide synthase activity)|go:"GO:0051966"(regulation of synaptic transmission, glutamatergic)|go:"GO:0060135"(maternal process involved in female pregnancy)|go:"GO:0060259"(regulation of feeding behavior)|go:"GO:0060405"(regulation of penile erection)|go:"GO:0098921"(retrograde trans-synaptic signaling by endocannabinoid)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|refseq:XP_011533726.1|refseq:XP_011533727.1|refseq:XP_011533728.1|refseq:XP_011533730.1|refseq:NP_001153698.1|refseq:NP_001153730.1|refseq:NP_001153731.1|refseq:NP_057167.2|refseq:NP_149421.2|refseq:XP_016865727.1|refseq:XP_016865728.1|refseq:XP_016865729.1|refseq:XP_006715393.1|dip:DIP-61575N - - - figure legend:Supplementary Fig 7B|agonist:"Quinpirole (D2L agonist) added concurrently with Arachidonyl-2'-chloroethylamide (ACEA) (a selective, synthetic agonist of CB1R), but only when untagged D2L was co-expressed. This effect was eradicated by addition of O-2050 -a small molecule cannabinoid derivative, acting as an antagonist for the CB1 receptor."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:11090(mouse-STHdhQ7/Q7)|taxid:11090(mouse STHdhQ7/Q7) - 2019/04/03 2019/07/04 rogid:fUnrdxwx4bBAqE3iO2cCB3UInyg9606 rogid:lqrlYa5VAmUZyZnutQlhneY1jLc9606 rigid:ikE1S4Z4c0LoQhiyy9K6oRrNWfU false renilla luciferase protein tag:188-189 green fluorescent protein tag:c-c - - psi-mi:"MI:0866"(tag visualisation) psi-mi:"MI:0866"(tag visualisation)