#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:P45758 uniprotkb:P45758 intact:EBI-21225187|uniprotkb:Q2M6Z0|ensemblbacteria:AAC76350|ensemblbacteria:BAE77966 intact:EBI-21225187|uniprotkb:Q2M6Z0|ensemblbacteria:AAC76350|ensemblbacteria:BAE77966 psi-mi:gspd_ecoli(display_long)|uniprotkb:Putative general secretion pathway protein D(gene name synonym)|uniprotkb:Putative type II secretion system protein D(gene name synonym)|uniprotkb:gspD(gene name)|psi-mi:gspD(display_short)|uniprotkb:yheF(gene name synonym)|uniprotkb:b3325(locus name)|uniprotkb:JW5707(locus name) psi-mi:gspd_ecoli(display_long)|uniprotkb:Putative general secretion pathway protein D(gene name synonym)|uniprotkb:Putative type II secretion system protein D(gene name synonym)|uniprotkb:gspD(gene name)|psi-mi:gspD(display_short)|uniprotkb:yheF(gene name synonym)|uniprotkb:b3325(locus name)|uniprotkb:JW5707(locus name) psi-mi:"MI:0410"(3D electron microscopy) Yan et al. (2017) pubmed:28067918|imex:IM-27023 taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0465"(DIP) intact:EBI-21399097|wwpdb:5wq7|emdb:EMD-6675|imex:IM-27023-13 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_417784.4|refseq:WP_001326512.1|rcsb pdb:5WQ7|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR005644(NolW-like)|interpro:IPR038591|interpro:IPR004846(Type II and III secretion system)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR004845(Type II secretion system gspD, conserved site)|ensemblbacteria:AAC76350(transcript)|ensemblbacteria:b3325(gene)|ensemblbacteria:BAE77966(gene)|ensemblbacteria:BAE77966(transcript)|go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding) refseq:NP_417784.4|refseq:WP_001326512.1|rcsb pdb:5WQ7|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR005644(NolW-like)|interpro:IPR038591|interpro:IPR004846(Type II and III secretion system)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR004845(Type II secretion system gspD, conserved site)|ensemblbacteria:AAC76350(transcript)|ensemblbacteria:b3325(gene)|ensemblbacteria:BAE77966(gene)|ensemblbacteria:BAE77966(transcript)|go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding) - - - figure legend:Figure1, Table1, Supplementary Figure 2|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") - 2019/02/27 2019/05/09 rogid:ztgP3MIAa88+8XuGfKwJ49amauY83333 rogid:ztgP3MIAa88+8XuGfKwJ49amauY83333 rigid:TAqL0Kc/0yMD3n80f3oZLAGsWow false strep tag:c-c|binding-associated region:24-650 strep tag:c-c|binding-associated region:24-650 15 0 psi-mi:"MI:2287"(identification by structure determination)|psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:2287"(identification by structure determination)|psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:P45779 uniprotkb:P45779 intact:EBI-6449570 intact:EBI-6449570 psi-mi:gspd_vibch(display_long)|uniprotkb:epsD(gene name)|psi-mi:epsD(display_short)|uniprotkb:Cholera toxin secretion protein EpsD(gene name synonym)|uniprotkb:General secretion pathway protein D(gene name synonym)|uniprotkb:Type II secretion system protein D(gene name synonym)|uniprotkb:VC_2733(locus name) psi-mi:gspd_vibch(display_long)|uniprotkb:epsD(gene name)|psi-mi:epsD(display_short)|uniprotkb:Cholera toxin secretion protein EpsD(gene name synonym)|uniprotkb:General secretion pathway protein D(gene name synonym)|uniprotkb:Type II secretion system protein D(gene name synonym)|uniprotkb:VC_2733(locus name) psi-mi:"MI:0410"(3D electron microscopy) Yan et al. (2017) pubmed:28067918|imex:IM-27023 taxid:243277(vibch)|taxid:243277(Vibrio cholerae) taxid:243277(vibch)|taxid:243277(Vibrio cholerae) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0465"(DIP) intact:EBI-21401453|emdb:EMD-6678|imex:IM-27023-15 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR004845(Type II secretion system gspD, conserved site)|interpro:IPR004846(Type II and III secretion system)|interpro:IPR005644(NolW-like)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR038591|rcsb pdb:5WQ8|rcsb pdb:5WQ9|dip:DIP-59035N|refseq:WP_000878557.1 go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR004845(Type II secretion system gspD, conserved site)|interpro:IPR004846(Type II and III secretion system)|interpro:IPR005644(NolW-like)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR038591|rcsb pdb:5WQ8|rcsb pdb:5WQ9|dip:DIP-59035N|refseq:WP_000878557.1 - crc64:3D77B891A59E6223 crc64:3D77B891A59E6223 figure legend:Figure1, Table1, Supplementary Figure 2|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") - 2019/02/27 2019/05/09 rogid:TTGtSpDUEpebDth5e8/R/zJI5Rg243277 rogid:TTGtSpDUEpebDth5e8/R/zJI5Rg243277 rigid:TwjUuthQ4cbvl1YD7fxeybpaCqg false mutation:371-393|strep tag:c-c|binding-associated region:25-674 mutation:371-393|strep tag:c-c|binding-associated region:25-674 15 0 psi-mi:"MI:2287"(identification by structure determination)|psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:2287"(identification by structure determination)|psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:P45779 uniprotkb:P45779 intact:EBI-6449570 intact:EBI-6449570 psi-mi:gspd_vibch(display_long)|uniprotkb:epsD(gene name)|psi-mi:epsD(display_short)|uniprotkb:Cholera toxin secretion protein EpsD(gene name synonym)|uniprotkb:General secretion pathway protein D(gene name synonym)|uniprotkb:Type II secretion system protein D(gene name synonym)|uniprotkb:VC_2733(locus name) psi-mi:gspd_vibch(display_long)|uniprotkb:epsD(gene name)|psi-mi:epsD(display_short)|uniprotkb:Cholera toxin secretion protein EpsD(gene name synonym)|uniprotkb:General secretion pathway protein D(gene name synonym)|uniprotkb:Type II secretion system protein D(gene name synonym)|uniprotkb:VC_2733(locus name) psi-mi:"MI:0410"(3D electron microscopy) Yan et al. (2017) pubmed:28067918|imex:IM-27023 taxid:243277(vibch)|taxid:243277(Vibrio cholerae) taxid:243277(vibch)|taxid:243277(Vibrio cholerae) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0465"(DIP) intact:EBI-21401480|wwpdb:5wq9|emdb:EMD-6677|imex:IM-27023-17 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR004845(Type II secretion system gspD, conserved site)|interpro:IPR004846(Type II and III secretion system)|interpro:IPR005644(NolW-like)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR038591|rcsb pdb:5WQ8|rcsb pdb:5WQ9|dip:DIP-59035N|refseq:WP_000878557.1 go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR004845(Type II secretion system gspD, conserved site)|interpro:IPR004846(Type II and III secretion system)|interpro:IPR005644(NolW-like)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR038591|rcsb pdb:5WQ8|rcsb pdb:5WQ9|dip:DIP-59035N|refseq:WP_000878557.1 - crc64:3D77B891A59E6223 crc64:3D77B891A59E6223 figure legend:Figure1, Table1, Supplementary Figure 2|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") - 2019/02/27 2019/05/09 rogid:TTGtSpDUEpebDth5e8/R/zJI5Rg243277 rogid:TTGtSpDUEpebDth5e8/R/zJI5Rg243277 rigid:TwjUuthQ4cbvl1YD7fxeybpaCqg false strep tag:c-c|mutation:477-477|binding-associated region:25-674 strep tag:c-c|mutation:477-477|binding-associated region:25-674 15 0 psi-mi:"MI:2287"(identification by structure determination)|psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:2287"(identification by structure determination)|psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:P45779 uniprotkb:P45779 intact:EBI-6449570 intact:EBI-6449570 psi-mi:gspd_vibch(display_long)|uniprotkb:epsD(gene name)|psi-mi:epsD(display_short)|uniprotkb:Cholera toxin secretion protein EpsD(gene name synonym)|uniprotkb:General secretion pathway protein D(gene name synonym)|uniprotkb:Type II secretion system protein D(gene name synonym)|uniprotkb:VC_2733(locus name) psi-mi:gspd_vibch(display_long)|uniprotkb:epsD(gene name)|psi-mi:epsD(display_short)|uniprotkb:Cholera toxin secretion protein EpsD(gene name synonym)|uniprotkb:General secretion pathway protein D(gene name synonym)|uniprotkb:Type II secretion system protein D(gene name synonym)|uniprotkb:VC_2733(locus name) psi-mi:"MI:0410"(3D electron microscopy) Yan et al. (2017) pubmed:28067918|imex:IM-27023 taxid:243277(vibch)|taxid:243277(Vibrio cholerae) taxid:243277(vibch)|taxid:243277(Vibrio cholerae) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0465"(DIP) intact:EBI-21401507|wwpdb:5wq8|emdb:EMD-6676|imex:IM-27023-19 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR004845(Type II secretion system gspD, conserved site)|interpro:IPR004846(Type II and III secretion system)|interpro:IPR005644(NolW-like)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR038591|rcsb pdb:5WQ8|rcsb pdb:5WQ9|dip:DIP-59035N|refseq:WP_000878557.1 go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR004845(Type II secretion system gspD, conserved site)|interpro:IPR004846(Type II and III secretion system)|interpro:IPR005644(NolW-like)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR038591|rcsb pdb:5WQ8|rcsb pdb:5WQ9|dip:DIP-59035N|refseq:WP_000878557.1 - crc64:3D77B891A59E6223 crc64:3D77B891A59E6223 figure legend:Figure1, Table1, Supplementary Figure 2|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") - 2019/02/27 2019/05/09 rogid:TTGtSpDUEpebDth5e8/R/zJI5Rg243277 rogid:TTGtSpDUEpebDth5e8/R/zJI5Rg243277 rigid:TwjUuthQ4cbvl1YD7fxeybpaCqg false strep tag:c-c|binding-associated region:25-674 strep tag:c-c|binding-associated region:25-674 15 0 psi-mi:"MI:2287"(identification by structure determination)|psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:2287"(identification by structure determination)|psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:P45758 uniprotkb:P45758 intact:EBI-21225187|uniprotkb:Q2M6Z0|ensemblbacteria:AAC76350|ensemblbacteria:BAE77966 intact:EBI-21225187|uniprotkb:Q2M6Z0|ensemblbacteria:AAC76350|ensemblbacteria:BAE77966 psi-mi:gspd_ecoli(display_long)|uniprotkb:Putative general secretion pathway protein D(gene name synonym)|uniprotkb:Putative type II secretion system protein D(gene name synonym)|uniprotkb:gspD(gene name)|psi-mi:gspD(display_short)|uniprotkb:yheF(gene name synonym)|uniprotkb:b3325(locus name)|uniprotkb:JW5707(locus name) psi-mi:gspd_ecoli(display_long)|uniprotkb:Putative general secretion pathway protein D(gene name synonym)|uniprotkb:Putative type II secretion system protein D(gene name synonym)|uniprotkb:gspD(gene name)|psi-mi:gspD(display_short)|uniprotkb:yheF(gene name synonym)|uniprotkb:b3325(locus name)|uniprotkb:JW5707(locus name) psi-mi:"MI:0071"(molecular sieving) Yan et al. (2017) pubmed:28067918|imex:IM-27023 taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0465"(DIP) intact:EBI-21225252|imex:IM-27023-21 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_417784.4|refseq:WP_001326512.1|rcsb pdb:5WQ7|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR005644(NolW-like)|interpro:IPR038591|interpro:IPR004846(Type II and III secretion system)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR004845(Type II secretion system gspD, conserved site)|ensemblbacteria:AAC76350(transcript)|ensemblbacteria:b3325(gene)|ensemblbacteria:BAE77966(gene)|ensemblbacteria:BAE77966(transcript)|go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051260"(protein homooligomerization) refseq:NP_417784.4|refseq:WP_001326512.1|rcsb pdb:5WQ7|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR005644(NolW-like)|interpro:IPR038591|interpro:IPR004846(Type II and III secretion system)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR004845(Type II secretion system gspD, conserved site)|ensemblbacteria:AAC76350(transcript)|ensemblbacteria:b3325(gene)|ensemblbacteria:BAE77966(gene)|ensemblbacteria:BAE77966(transcript)|go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding) go:"GO:0051260"(protein homooligomerization) - - figure legend:Supplementary Figure 1c|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") - 2019/02/27 2019/02/27 rogid:ztgP3MIAa88+8XuGfKwJ49amauY83333 rogid:ztgP3MIAa88+8XuGfKwJ49amauY83333 rigid:OMWkMh9oFTYjoQuqhbTMKb7c1zo false strep tag:c-c|binding-associated region:24-650 strep tag:c-c|binding-associated region:24-650 - - psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:P45779 uniprotkb:P45779 intact:EBI-6449570 intact:EBI-6449570 psi-mi:gspd_vibch(display_long)|uniprotkb:epsD(gene name)|psi-mi:epsD(display_short)|uniprotkb:Cholera toxin secretion protein EpsD(gene name synonym)|uniprotkb:General secretion pathway protein D(gene name synonym)|uniprotkb:Type II secretion system protein D(gene name synonym)|uniprotkb:VC_2733(locus name) psi-mi:gspd_vibch(display_long)|uniprotkb:epsD(gene name)|psi-mi:epsD(display_short)|uniprotkb:Cholera toxin secretion protein EpsD(gene name synonym)|uniprotkb:General secretion pathway protein D(gene name synonym)|uniprotkb:Type II secretion system protein D(gene name synonym)|uniprotkb:VC_2733(locus name) psi-mi:"MI:0071"(molecular sieving) Yan et al. (2017) pubmed:28067918|imex:IM-27023 taxid:243277(vibch)|taxid:243277(Vibrio cholerae) taxid:243277(vibch)|taxid:243277(Vibrio cholerae) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0465"(DIP) intact:EBI-21225266|imex:IM-27023-23 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR004845(Type II secretion system gspD, conserved site)|interpro:IPR004846(Type II and III secretion system)|interpro:IPR005644(NolW-like)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR038591|rcsb pdb:5WQ8|rcsb pdb:5WQ9|dip:DIP-59035N|refseq:WP_000878557.1 go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR004845(Type II secretion system gspD, conserved site)|interpro:IPR004846(Type II and III secretion system)|interpro:IPR005644(NolW-like)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR038591|rcsb pdb:5WQ8|rcsb pdb:5WQ9|dip:DIP-59035N|refseq:WP_000878557.1 go:"GO:0051260"(protein homooligomerization) crc64:3D77B891A59E6223 crc64:3D77B891A59E6223 figure legend:Supplementary Figure 1e|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") - 2019/02/27 2019/02/27 rogid:TTGtSpDUEpebDth5e8/R/zJI5Rg243277 rogid:TTGtSpDUEpebDth5e8/R/zJI5Rg243277 rigid:CMHW4LPyTLLlfT7QJ8DLToB/6kk false strep tag:c-c|binding-associated region:25-674 strep tag:c-c|binding-associated region:25-674 - - psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:P45779 uniprotkb:P45779 intact:EBI-6449570 intact:EBI-6449570 psi-mi:gspd_vibch(display_long)|uniprotkb:epsD(gene name)|psi-mi:epsD(display_short)|uniprotkb:Cholera toxin secretion protein EpsD(gene name synonym)|uniprotkb:General secretion pathway protein D(gene name synonym)|uniprotkb:Type II secretion system protein D(gene name synonym)|uniprotkb:VC_2733(locus name) psi-mi:gspd_vibch(display_long)|uniprotkb:epsD(gene name)|psi-mi:epsD(display_short)|uniprotkb:Cholera toxin secretion protein EpsD(gene name synonym)|uniprotkb:General secretion pathway protein D(gene name synonym)|uniprotkb:Type II secretion system protein D(gene name synonym)|uniprotkb:VC_2733(locus name) psi-mi:"MI:0071"(molecular sieving) Yan et al. (2017) pubmed:28067918|imex:IM-27023 taxid:243277(vibch)|taxid:243277(Vibrio cholerae) taxid:243277(vibch)|taxid:243277(Vibrio cholerae) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0465"(DIP) intact:EBI-21225331|imex:IM-27023-25 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR004845(Type II secretion system gspD, conserved site)|interpro:IPR004846(Type II and III secretion system)|interpro:IPR005644(NolW-like)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR038591|rcsb pdb:5WQ8|rcsb pdb:5WQ9|dip:DIP-59035N|refseq:WP_000878557.1 go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR004845(Type II secretion system gspD, conserved site)|interpro:IPR004846(Type II and III secretion system)|interpro:IPR005644(NolW-like)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR038591|rcsb pdb:5WQ8|rcsb pdb:5WQ9|dip:DIP-59035N|refseq:WP_000878557.1 go:"GO:0051260"(protein homooligomerization) crc64:3D77B891A59E6223 crc64:3D77B891A59E6223 figure legend:Supplementary Figure 1f|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") - 2019/02/27 2019/02/27 rogid:TTGtSpDUEpebDth5e8/R/zJI5Rg243277 rogid:TTGtSpDUEpebDth5e8/R/zJI5Rg243277 rigid:CMHW4LPyTLLlfT7QJ8DLToB/6kk false strep tag:n-n|binding-associated region:25-674 strep tag:n-n|binding-associated region:25-674 - - psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:P45758 uniprotkb:P45758 intact:EBI-21225187|uniprotkb:Q2M6Z0|ensemblbacteria:AAC76350|ensemblbacteria:BAE77966 intact:EBI-21225187|uniprotkb:Q2M6Z0|ensemblbacteria:AAC76350|ensemblbacteria:BAE77966 psi-mi:gspd_ecoli(display_long)|uniprotkb:Putative general secretion pathway protein D(gene name synonym)|uniprotkb:Putative type II secretion system protein D(gene name synonym)|uniprotkb:gspD(gene name)|psi-mi:gspD(display_short)|uniprotkb:yheF(gene name synonym)|uniprotkb:b3325(locus name)|uniprotkb:JW5707(locus name) psi-mi:gspd_ecoli(display_long)|uniprotkb:Putative general secretion pathway protein D(gene name synonym)|uniprotkb:Putative type II secretion system protein D(gene name synonym)|uniprotkb:gspD(gene name)|psi-mi:gspD(display_short)|uniprotkb:yheF(gene name synonym)|uniprotkb:b3325(locus name)|uniprotkb:JW5707(locus name) psi-mi:"MI:0071"(molecular sieving) Yan et al. (2017) pubmed:28067918|imex:IM-27023 taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0465"(DIP) intact:EBI-21225346|imex:IM-27023-27 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_417784.4|refseq:WP_001326512.1|rcsb pdb:5WQ7|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR005644(NolW-like)|interpro:IPR038591|interpro:IPR004846(Type II and III secretion system)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR004845(Type II secretion system gspD, conserved site)|ensemblbacteria:AAC76350(transcript)|ensemblbacteria:b3325(gene)|ensemblbacteria:BAE77966(gene)|ensemblbacteria:BAE77966(transcript)|go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051260"(protein homooligomerization) refseq:NP_417784.4|refseq:WP_001326512.1|rcsb pdb:5WQ7|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR005644(NolW-like)|interpro:IPR038591|interpro:IPR004846(Type II and III secretion system)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR004845(Type II secretion system gspD, conserved site)|ensemblbacteria:AAC76350(transcript)|ensemblbacteria:b3325(gene)|ensemblbacteria:BAE77966(gene)|ensemblbacteria:BAE77966(transcript)|go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding) go:"GO:0051260"(protein homooligomerization) - - figure legend:Supplementary Figure 1d|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") - 2019/02/27 2019/02/27 rogid:ztgP3MIAa88+8XuGfKwJ49amauY83333 rogid:ztgP3MIAa88+8XuGfKwJ49amauY83333 rigid:OMWkMh9oFTYjoQuqhbTMKb7c1zo false strep tag:n-n|binding-associated region:24-650 strep tag:n-n|binding-associated region:24-650 - - psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:P45779 uniprotkb:P45779 intact:EBI-6449570 intact:EBI-6449570 psi-mi:gspd_vibch(display_long)|uniprotkb:epsD(gene name)|psi-mi:epsD(display_short)|uniprotkb:Cholera toxin secretion protein EpsD(gene name synonym)|uniprotkb:General secretion pathway protein D(gene name synonym)|uniprotkb:Type II secretion system protein D(gene name synonym)|uniprotkb:VC_2733(locus name) psi-mi:gspd_vibch(display_long)|uniprotkb:epsD(gene name)|psi-mi:epsD(display_short)|uniprotkb:Cholera toxin secretion protein EpsD(gene name synonym)|uniprotkb:General secretion pathway protein D(gene name synonym)|uniprotkb:Type II secretion system protein D(gene name synonym)|uniprotkb:VC_2733(locus name) psi-mi:"MI:0808"(comigration in sds page) Yan et al. (2017) pubmed:28067918|imex:IM-27023 taxid:243277(vibch)|taxid:243277(Vibrio cholerae) taxid:243277(vibch)|taxid:243277(Vibrio cholerae) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0465"(DIP) intact:EBI-21229122|imex:IM-27023-1 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR004845(Type II secretion system gspD, conserved site)|interpro:IPR004846(Type II and III secretion system)|interpro:IPR005644(NolW-like)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR038591|rcsb pdb:5WQ8|rcsb pdb:5WQ9|dip:DIP-59035N|refseq:WP_000878557.1 go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR004845(Type II secretion system gspD, conserved site)|interpro:IPR004846(Type II and III secretion system)|interpro:IPR005644(NolW-like)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR038591|rcsb pdb:5WQ8|rcsb pdb:5WQ9|dip:DIP-59035N|refseq:WP_000878557.1 go:"GO:0051260"(protein homooligomerization) crc64:3D77B891A59E6223 crc64:3D77B891A59E6223 figure legend:"Supplementary Figure 7b (L484D lane)"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") - 2019/02/27 2019/03/05 rogid:TTGtSpDUEpebDth5e8/R/zJI5Rg243277 rogid:TTGtSpDUEpebDth5e8/R/zJI5Rg243277 rigid:CMHW4LPyTLLlfT7QJ8DLToB/6kk false strep tag:c-c|mutation disrupting interaction:508-508|binding-associated region:25-674 strep tag:c-c|mutation disrupting interaction:508-508|binding-associated region:25-674 - - psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:P45779 uniprotkb:P45779 intact:EBI-6449570 intact:EBI-6449570 psi-mi:gspd_vibch(display_long)|uniprotkb:epsD(gene name)|psi-mi:epsD(display_short)|uniprotkb:Cholera toxin secretion protein EpsD(gene name synonym)|uniprotkb:General secretion pathway protein D(gene name synonym)|uniprotkb:Type II secretion system protein D(gene name synonym)|uniprotkb:VC_2733(locus name) psi-mi:gspd_vibch(display_long)|uniprotkb:epsD(gene name)|psi-mi:epsD(display_short)|uniprotkb:Cholera toxin secretion protein EpsD(gene name synonym)|uniprotkb:General secretion pathway protein D(gene name synonym)|uniprotkb:Type II secretion system protein D(gene name synonym)|uniprotkb:VC_2733(locus name) psi-mi:"MI:0808"(comigration in sds page) Yan et al. (2017) pubmed:28067918|imex:IM-27023 taxid:243277(vibch)|taxid:243277(Vibrio cholerae) taxid:243277(vibch)|taxid:243277(Vibrio cholerae) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0465"(DIP) intact:EBI-21229145|imex:IM-27023-3 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR004845(Type II secretion system gspD, conserved site)|interpro:IPR004846(Type II and III secretion system)|interpro:IPR005644(NolW-like)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR038591|rcsb pdb:5WQ8|rcsb pdb:5WQ9|dip:DIP-59035N|refseq:WP_000878557.1 go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR004845(Type II secretion system gspD, conserved site)|interpro:IPR004846(Type II and III secretion system)|interpro:IPR005644(NolW-like)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR038591|rcsb pdb:5WQ8|rcsb pdb:5WQ9|dip:DIP-59035N|refseq:WP_000878557.1 go:"GO:0051260"(protein homooligomerization) crc64:3D77B891A59E6223 crc64:3D77B891A59E6223 figure legend:"Supplementary Figure 7b (V486D lane)"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") - 2019/02/27 2019/03/05 rogid:TTGtSpDUEpebDth5e8/R/zJI5Rg243277 rogid:TTGtSpDUEpebDth5e8/R/zJI5Rg243277 rigid:CMHW4LPyTLLlfT7QJ8DLToB/6kk false strep tag:c-c|mutation disrupting interaction:510-510|binding-associated region:25-674 strep tag:c-c|mutation disrupting interaction:510-510|binding-associated region:25-674 - - psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:P45779 uniprotkb:P45779 intact:EBI-6449570 intact:EBI-6449570 psi-mi:gspd_vibch(display_long)|uniprotkb:epsD(gene name)|psi-mi:epsD(display_short)|uniprotkb:Cholera toxin secretion protein EpsD(gene name synonym)|uniprotkb:General secretion pathway protein D(gene name synonym)|uniprotkb:Type II secretion system protein D(gene name synonym)|uniprotkb:VC_2733(locus name) psi-mi:gspd_vibch(display_long)|uniprotkb:epsD(gene name)|psi-mi:epsD(display_short)|uniprotkb:Cholera toxin secretion protein EpsD(gene name synonym)|uniprotkb:General secretion pathway protein D(gene name synonym)|uniprotkb:Type II secretion system protein D(gene name synonym)|uniprotkb:VC_2733(locus name) psi-mi:"MI:0808"(comigration in sds page) Yan et al. (2017) pubmed:28067918|imex:IM-27023 taxid:243277(vibch)|taxid:243277(Vibrio cholerae) taxid:243277(vibch)|taxid:243277(Vibrio cholerae) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0465"(DIP) intact:EBI-21229160|imex:IM-27023-5 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR004845(Type II secretion system gspD, conserved site)|interpro:IPR004846(Type II and III secretion system)|interpro:IPR005644(NolW-like)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR038591|rcsb pdb:5WQ8|rcsb pdb:5WQ9|dip:DIP-59035N|refseq:WP_000878557.1 go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR004845(Type II secretion system gspD, conserved site)|interpro:IPR004846(Type II and III secretion system)|interpro:IPR005644(NolW-like)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR038591|rcsb pdb:5WQ8|rcsb pdb:5WQ9|dip:DIP-59035N|refseq:WP_000878557.1 go:"GO:0051260"(protein homooligomerization) crc64:3D77B891A59E6223 crc64:3D77B891A59E6223 figure legend:"Supplementary Figure 7b (L498D lane)"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") - 2019/02/27 2019/03/05 rogid:TTGtSpDUEpebDth5e8/R/zJI5Rg243277 rogid:TTGtSpDUEpebDth5e8/R/zJI5Rg243277 rigid:CMHW4LPyTLLlfT7QJ8DLToB/6kk false strep tag:c-c|mutation disrupting interaction:522-522|binding-associated region:25-674 strep tag:c-c|mutation disrupting interaction:522-522|binding-associated region:25-674 - - psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:P45779 uniprotkb:P45779 intact:EBI-6449570 intact:EBI-6449570 psi-mi:gspd_vibch(display_long)|uniprotkb:epsD(gene name)|psi-mi:epsD(display_short)|uniprotkb:Cholera toxin secretion protein EpsD(gene name synonym)|uniprotkb:General secretion pathway protein D(gene name synonym)|uniprotkb:Type II secretion system protein D(gene name synonym)|uniprotkb:VC_2733(locus name) psi-mi:gspd_vibch(display_long)|uniprotkb:epsD(gene name)|psi-mi:epsD(display_short)|uniprotkb:Cholera toxin secretion protein EpsD(gene name synonym)|uniprotkb:General secretion pathway protein D(gene name synonym)|uniprotkb:Type II secretion system protein D(gene name synonym)|uniprotkb:VC_2733(locus name) psi-mi:"MI:0808"(comigration in sds page) Yan et al. (2017) pubmed:28067918|imex:IM-27023 taxid:243277(vibch)|taxid:243277(Vibrio cholerae) taxid:243277(vibch)|taxid:243277(Vibrio cholerae) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0465"(DIP) intact:EBI-21229175|imex:IM-27023-7 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR004845(Type II secretion system gspD, conserved site)|interpro:IPR004846(Type II and III secretion system)|interpro:IPR005644(NolW-like)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR038591|rcsb pdb:5WQ8|rcsb pdb:5WQ9|dip:DIP-59035N|refseq:WP_000878557.1 go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR004845(Type II secretion system gspD, conserved site)|interpro:IPR004846(Type II and III secretion system)|interpro:IPR005644(NolW-like)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR038591|rcsb pdb:5WQ8|rcsb pdb:5WQ9|dip:DIP-59035N|refseq:WP_000878557.1 go:"GO:0051260"(protein homooligomerization) crc64:3D77B891A59E6223 crc64:3D77B891A59E6223 figure legend:"Supplementary Figure 7b (I500E lane)"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") - 2019/02/27 2019/03/05 rogid:TTGtSpDUEpebDth5e8/R/zJI5Rg243277 rogid:TTGtSpDUEpebDth5e8/R/zJI5Rg243277 rigid:CMHW4LPyTLLlfT7QJ8DLToB/6kk false strep tag:c-c|mutation disrupting interaction:524-524|binding-associated region:25-674 strep tag:c-c|mutation disrupting interaction:524-524|binding-associated region:25-674 - - psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:P45779 uniprotkb:P45779 intact:EBI-6449570 intact:EBI-6449570 psi-mi:gspd_vibch(display_long)|uniprotkb:epsD(gene name)|psi-mi:epsD(display_short)|uniprotkb:Cholera toxin secretion protein EpsD(gene name synonym)|uniprotkb:General secretion pathway protein D(gene name synonym)|uniprotkb:Type II secretion system protein D(gene name synonym)|uniprotkb:VC_2733(locus name) psi-mi:gspd_vibch(display_long)|uniprotkb:epsD(gene name)|psi-mi:epsD(display_short)|uniprotkb:Cholera toxin secretion protein EpsD(gene name synonym)|uniprotkb:General secretion pathway protein D(gene name synonym)|uniprotkb:Type II secretion system protein D(gene name synonym)|uniprotkb:VC_2733(locus name) psi-mi:"MI:0808"(comigration in sds page) Yan et al. (2017) pubmed:28067918|imex:IM-27023 taxid:243277(vibch)|taxid:243277(Vibrio cholerae) taxid:243277(vibch)|taxid:243277(Vibrio cholerae) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0465"(DIP) intact:EBI-21229190|imex:IM-27023-29 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR004845(Type II secretion system gspD, conserved site)|interpro:IPR004846(Type II and III secretion system)|interpro:IPR005644(NolW-like)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR038591|rcsb pdb:5WQ8|rcsb pdb:5WQ9|dip:DIP-59035N|refseq:WP_000878557.1 go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR004845(Type II secretion system gspD, conserved site)|interpro:IPR004846(Type II and III secretion system)|interpro:IPR005644(NolW-like)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR038591|rcsb pdb:5WQ8|rcsb pdb:5WQ9|dip:DIP-59035N|refseq:WP_000878557.1 go:"GO:0051260"(protein homooligomerization) crc64:3D77B891A59E6223 crc64:3D77B891A59E6223 figure legend:"Supplementary Figure 7b (I581E lane)"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") - 2019/02/27 2019/03/05 rogid:TTGtSpDUEpebDth5e8/R/zJI5Rg243277 rogid:TTGtSpDUEpebDth5e8/R/zJI5Rg243277 rigid:CMHW4LPyTLLlfT7QJ8DLToB/6kk false strep tag:c-c|mutation disrupting interaction:605-605|binding-associated region:25-674 strep tag:c-c|mutation disrupting interaction:605-605|binding-associated region:25-674 - - psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:P45779 uniprotkb:P45779 intact:EBI-6449570 intact:EBI-6449570 psi-mi:gspd_vibch(display_long)|uniprotkb:epsD(gene name)|psi-mi:epsD(display_short)|uniprotkb:Cholera toxin secretion protein EpsD(gene name synonym)|uniprotkb:General secretion pathway protein D(gene name synonym)|uniprotkb:Type II secretion system protein D(gene name synonym)|uniprotkb:VC_2733(locus name) psi-mi:gspd_vibch(display_long)|uniprotkb:epsD(gene name)|psi-mi:epsD(display_short)|uniprotkb:Cholera toxin secretion protein EpsD(gene name synonym)|uniprotkb:General secretion pathway protein D(gene name synonym)|uniprotkb:Type II secretion system protein D(gene name synonym)|uniprotkb:VC_2733(locus name) psi-mi:"MI:0808"(comigration in sds page) Yan et al. (2017) pubmed:28067918|imex:IM-27023 taxid:243277(vibch)|taxid:243277(Vibrio cholerae) taxid:243277(vibch)|taxid:243277(Vibrio cholerae) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0465"(DIP) intact:EBI-21229205|imex:IM-27023-9 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR004845(Type II secretion system gspD, conserved site)|interpro:IPR004846(Type II and III secretion system)|interpro:IPR005644(NolW-like)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR038591|rcsb pdb:5WQ8|rcsb pdb:5WQ9|dip:DIP-59035N|refseq:WP_000878557.1 go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR004845(Type II secretion system gspD, conserved site)|interpro:IPR004846(Type II and III secretion system)|interpro:IPR005644(NolW-like)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR038591|rcsb pdb:5WQ8|rcsb pdb:5WQ9|dip:DIP-59035N|refseq:WP_000878557.1 go:"GO:0051260"(protein homooligomerization) crc64:3D77B891A59E6223 crc64:3D77B891A59E6223 figure legend:"Supplementary Figure 7c (G453A lane)"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") - 2019/02/27 2019/03/05 rogid:TTGtSpDUEpebDth5e8/R/zJI5Rg243277 rogid:TTGtSpDUEpebDth5e8/R/zJI5Rg243277 rigid:CMHW4LPyTLLlfT7QJ8DLToB/6kk false strep tag:c-c|mutation decreasing interaction:477-477|binding-associated region:25-674 strep tag:c-c|mutation decreasing interaction:477-477|binding-associated region:25-674 - - psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:P45779 uniprotkb:P45779 intact:EBI-6449570 intact:EBI-6449570 psi-mi:gspd_vibch(display_long)|uniprotkb:epsD(gene name)|psi-mi:epsD(display_short)|uniprotkb:Cholera toxin secretion protein EpsD(gene name synonym)|uniprotkb:General secretion pathway protein D(gene name synonym)|uniprotkb:Type II secretion system protein D(gene name synonym)|uniprotkb:VC_2733(locus name) psi-mi:gspd_vibch(display_long)|uniprotkb:epsD(gene name)|psi-mi:epsD(display_short)|uniprotkb:Cholera toxin secretion protein EpsD(gene name synonym)|uniprotkb:General secretion pathway protein D(gene name synonym)|uniprotkb:Type II secretion system protein D(gene name synonym)|uniprotkb:VC_2733(locus name) psi-mi:"MI:0808"(comigration in sds page) Yan et al. (2017) pubmed:28067918|imex:IM-27023 taxid:243277(vibch)|taxid:243277(Vibrio cholerae) taxid:243277(vibch)|taxid:243277(Vibrio cholerae) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0465"(DIP) intact:EBI-21229220|imex:IM-27023-31 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR004845(Type II secretion system gspD, conserved site)|interpro:IPR004846(Type II and III secretion system)|interpro:IPR005644(NolW-like)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR038591|rcsb pdb:5WQ8|rcsb pdb:5WQ9|dip:DIP-59035N|refseq:WP_000878557.1 go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR004845(Type II secretion system gspD, conserved site)|interpro:IPR004846(Type II and III secretion system)|interpro:IPR005644(NolW-like)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR038591|rcsb pdb:5WQ8|rcsb pdb:5WQ9|dip:DIP-59035N|refseq:WP_000878557.1 go:"GO:0051260"(protein homooligomerization) crc64:3D77B891A59E6223 crc64:3D77B891A59E6223 figure legend:"Supplementary Figure 7c (G481A lane)"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") - 2019/02/27 2019/03/05 rogid:TTGtSpDUEpebDth5e8/R/zJI5Rg243277 rogid:TTGtSpDUEpebDth5e8/R/zJI5Rg243277 rigid:CMHW4LPyTLLlfT7QJ8DLToB/6kk false strep tag:c-c|mutation disrupting interaction:505-505|binding-associated region:25-674 strep tag:c-c|mutation disrupting interaction:505-505|binding-associated region:25-674 - - psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:P45779 uniprotkb:P45779 intact:EBI-6449570 intact:EBI-6449570 psi-mi:gspd_vibch(display_long)|uniprotkb:epsD(gene name)|psi-mi:epsD(display_short)|uniprotkb:Cholera toxin secretion protein EpsD(gene name synonym)|uniprotkb:General secretion pathway protein D(gene name synonym)|uniprotkb:Type II secretion system protein D(gene name synonym)|uniprotkb:VC_2733(locus name) psi-mi:gspd_vibch(display_long)|uniprotkb:epsD(gene name)|psi-mi:epsD(display_short)|uniprotkb:Cholera toxin secretion protein EpsD(gene name synonym)|uniprotkb:General secretion pathway protein D(gene name synonym)|uniprotkb:Type II secretion system protein D(gene name synonym)|uniprotkb:VC_2733(locus name) psi-mi:"MI:0808"(comigration in sds page) Yan et al. (2017) pubmed:28067918|imex:IM-27023 taxid:243277(vibch)|taxid:243277(Vibrio cholerae) taxid:243277(vibch)|taxid:243277(Vibrio cholerae) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0465"(DIP) intact:EBI-21229235|imex:IM-27023-11 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR004845(Type II secretion system gspD, conserved site)|interpro:IPR004846(Type II and III secretion system)|interpro:IPR005644(NolW-like)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR038591|rcsb pdb:5WQ8|rcsb pdb:5WQ9|dip:DIP-59035N|refseq:WP_000878557.1 go:"GO:0009279"(cell outer membrane)|go:"GO:0009306"(protein secretion)|go:"GO:0015627"(type II protein secretion system complex)|go:"GO:0015628"(protein secretion by the type II secretion system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR001775(Bacterial general secretion pathway protein D)|interpro:IPR004845(Type II secretion system gspD, conserved site)|interpro:IPR004846(Type II and III secretion system)|interpro:IPR005644(NolW-like)|interpro:IPR013356(Type II secretion system gspD)|interpro:IPR038591|rcsb pdb:5WQ8|rcsb pdb:5WQ9|dip:DIP-59035N|refseq:WP_000878557.1 go:"GO:0051260"(protein homooligomerization) crc64:3D77B891A59E6223 crc64:3D77B891A59E6223 figure legend:"Supplementary Figure 7c (G453A&G481A lane)"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") - 2019/02/27 2019/03/05 rogid:TTGtSpDUEpebDth5e8/R/zJI5Rg243277 rogid:TTGtSpDUEpebDth5e8/R/zJI5Rg243277 rigid:CMHW4LPyTLLlfT7QJ8DLToB/6kk false mutation disrupting interaction:477-477,505-505|strep tag:c-c|binding-associated region:25-674 strep tag:c-c|mutation disrupting interaction:477-477,505-505|binding-associated region:25-674 - - psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry)