#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P43681	uniprotkb:Q6UXH1-1	intact:EBI-7132379|ensembl:ENSP00000359285|uniprotkb:Q4JGR7|intact:MINT-3015931|uniprotkb:Q4VAQ6|intact:MINT-4869480|uniprotkb:Q4VAQ5	intact:EBI-21348071|ensembl:ENSP00000332223	psi-mi:acha4_human(display_long)|uniprotkb:CHRNA4(gene name)|psi-mi:CHRNA4(display_short)|uniprotkb:NACRA4(gene name synonym)	psi-mi:q6uxh1-1(display_long)|uniprotkb:CRELD2(gene name)|psi-mi:CRELD2(display_short)|uniprotkb:Cysteine-rich with EGF-like domain protein 2(gene name synonym)|uniprotkb:UNQ185/PRO211(orf name)|uniprotkb:Delta(isoform synonym)	psi-mi:"MI:0727"(lexa b52 complementation)	Ortiz et al. (2005)	pubmed:16238698|imex:IM-26962	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-21348061|imex:IM-26962-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:XP_011526826.1|go:"GO:0006979"(response to oxidative stress)|go:"GO:0007165"(signal transduction)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007271"(synaptic transmission, cholinergic)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0014059"(regulation of dopamine secretion)|go:"GO:0015276"(ligand-gated ion channel activity)|go:"GO:0015464"(acetylcholine receptor activity)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0030425"(dendrite)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0035094"(response to nicotine)|go:"GO:0035095"(behavioral response to nicotine)|go:"GO:0042113"(B cell activation)|go:"GO:0042166"(acetylcholine binding)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0043005"(neuron projection)|go:"GO:0043025"(neuronal cell body)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0050877"(nervous system process)|go:"GO:0050890"(cognition)|go:"GO:0051899"(membrane depolarization)|go:"GO:0060080"(inhibitory postsynaptic potential)|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR018000|interpro:IPR036719|interpro:IPR036734|rcsb pdb:2GVT|rcsb pdb:2LLY|rcsb pdb:5KXI|rcsb pdb:6CNJ|rcsb pdb:6CNK|rcsb pdb:6UR8|rcsb pdb:6USF|reactome:R-HSA-629587|reactome:R-HSA-629594|reactome:R-HSA-629597|ensembl:ENSG00000101204(gene)|ensembl:ENST00000370263(transcript)|go:"GO:0001508"(action potential)|go:"GO:0001666"(response to hypoxia)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005892"(acetylcholine-gated channel complex)|go:"GO:0006281"(DNA repair)|go:"GO:0006811"(ion transport)|go:"GO:0006816"(calcium ion transport)|refseq:NP_001243502.1|refseq:NP_000735.1	ensembl:ENSG00000184164(gene)|ensembl:ENST00000328268(transcript)|intact:EBI-3935314(isoform-parent)	-	comment:mint|comment:homomint|function:After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane|function:"Defects in CHRNA4 are the cause of autosomal dominant nocturnal frontal lobe epilepsy type 1 (ENFL1) [MIM:600513]; also symbolized ADNFLE. ENFL1 is a disease characterized by clusters of motor seizures during sleep"	-	figure legend:no figure|comment:"GenBank accession number NP_077300. (\"alpha\" isoform)"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	-	2019/04/09	2019/04/23	rogid:1mIuzGVLlNQeGoCu/fI+lbDZir49606	rogid:MJtefM+QiJRIn/HGdEr6vEd4bB49606	rigid:6L8uQVxNSye/fY6JtHlmzQrUheM	false	sufficient binding region:331-600	-	-	-	psi-mi:"MI:0078"(nucleotide sequence identification)	psi-mi:"MI:0078"(nucleotide sequence identification)
uniprotkb:P43681	uniprotkb:Q6UXH1-3	intact:EBI-7132379|ensembl:ENSP00000359285|uniprotkb:Q4JGR7|intact:MINT-3015931|uniprotkb:Q4VAQ6|intact:MINT-4869480|uniprotkb:Q4VAQ5	intact:EBI-21348090	psi-mi:acha4_human(display_long)|uniprotkb:CHRNA4(gene name)|psi-mi:CHRNA4(display_short)|uniprotkb:NACRA4(gene name synonym)	psi-mi:q6uxh1-3(display_long)|uniprotkb:CRELD2(gene name)|psi-mi:CRELD2(display_short)|uniprotkb:Cysteine-rich with EGF-like domain protein 2(gene name synonym)|uniprotkb:UNQ185/PRO211(orf name)|uniprotkb:Beta(isoform synonym)	psi-mi:"MI:0727"(lexa b52 complementation)	Ortiz et al. (2005)	pubmed:16238698|imex:IM-26962	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-21348098|imex:IM-26962-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:XP_011526826.1|go:"GO:0006979"(response to oxidative stress)|go:"GO:0007165"(signal transduction)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007271"(synaptic transmission, cholinergic)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0014059"(regulation of dopamine secretion)|go:"GO:0015276"(ligand-gated ion channel activity)|go:"GO:0015464"(acetylcholine receptor activity)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0030425"(dendrite)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0035094"(response to nicotine)|go:"GO:0035095"(behavioral response to nicotine)|go:"GO:0042113"(B cell activation)|go:"GO:0042166"(acetylcholine binding)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0043005"(neuron projection)|go:"GO:0043025"(neuronal cell body)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0050877"(nervous system process)|go:"GO:0050890"(cognition)|go:"GO:0051899"(membrane depolarization)|go:"GO:0060080"(inhibitory postsynaptic potential)|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR018000|interpro:IPR036719|interpro:IPR036734|rcsb pdb:2GVT|rcsb pdb:2LLY|rcsb pdb:5KXI|rcsb pdb:6CNJ|rcsb pdb:6CNK|rcsb pdb:6UR8|rcsb pdb:6USF|reactome:R-HSA-629587|reactome:R-HSA-629594|reactome:R-HSA-629597|ensembl:ENSG00000101204(gene)|ensembl:ENST00000370263(transcript)|go:"GO:0001508"(action potential)|go:"GO:0001666"(response to hypoxia)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005892"(acetylcholine-gated channel complex)|go:"GO:0006281"(DNA repair)|go:"GO:0006811"(ion transport)|go:"GO:0006816"(calcium ion transport)|refseq:NP_001243502.1|refseq:NP_000735.1	intact:EBI-3935314(isoform-parent)	-	comment:mint|comment:homomint|function:After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane|function:"Defects in CHRNA4 are the cause of autosomal dominant nocturnal frontal lobe epilepsy type 1 (ENFL1) [MIM:600513]; also symbolized ADNFLE. ENFL1 is a disease characterized by clusters of motor seizures during sleep"	isoform-comment:Minor isoform.	figure legend:no figure|comment:"Clone B260, \"beta\" isoform. (Genbank identifier: AJ968414)"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	-	2019/04/09	2019/04/23	rogid:1mIuzGVLlNQeGoCu/fI+lbDZir49606	rogid:Pct6qcuvej1ixuinF8phSdFhLis9606	rigid:kj5mPEAUJkopVis/ZL6b1vfeVAE	false	sufficient binding region:331-600	-	-	-	psi-mi:"MI:0078"(nucleotide sequence identification)	psi-mi:"MI:0078"(nucleotide sequence identification)
uniprotkb:P43681	uniprotkb:Q6UXH1-1	intact:EBI-7132379|ensembl:ENSP00000359285|uniprotkb:Q4JGR7|intact:MINT-3015931|uniprotkb:Q4VAQ6|intact:MINT-4869480|uniprotkb:Q4VAQ5	intact:EBI-21348071|ensembl:ENSP00000332223	psi-mi:acha4_human(display_long)|uniprotkb:CHRNA4(gene name)|psi-mi:CHRNA4(display_short)|uniprotkb:NACRA4(gene name synonym)	psi-mi:q6uxh1-1(display_long)|uniprotkb:CRELD2(gene name)|psi-mi:CRELD2(display_short)|uniprotkb:Cysteine-rich with EGF-like domain protein 2(gene name synonym)|uniprotkb:UNQ185/PRO211(orf name)|uniprotkb:Delta(isoform synonym)	psi-mi:"MI:0727"(lexa b52 complementation)	Ortiz et al. (2005)	pubmed:16238698|imex:IM-26962	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-21348119|imex:IM-26962-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:XP_011526826.1|go:"GO:0006979"(response to oxidative stress)|go:"GO:0007165"(signal transduction)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007271"(synaptic transmission, cholinergic)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0014059"(regulation of dopamine secretion)|go:"GO:0015276"(ligand-gated ion channel activity)|go:"GO:0015464"(acetylcholine receptor activity)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0030425"(dendrite)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0035094"(response to nicotine)|go:"GO:0035095"(behavioral response to nicotine)|go:"GO:0042113"(B cell activation)|go:"GO:0042166"(acetylcholine binding)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0043005"(neuron projection)|go:"GO:0043025"(neuronal cell body)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0050877"(nervous system process)|go:"GO:0050890"(cognition)|go:"GO:0051899"(membrane depolarization)|go:"GO:0060080"(inhibitory postsynaptic potential)|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR018000|interpro:IPR036719|interpro:IPR036734|rcsb pdb:2GVT|rcsb pdb:2LLY|rcsb pdb:5KXI|rcsb pdb:6CNJ|rcsb pdb:6CNK|rcsb pdb:6UR8|rcsb pdb:6USF|reactome:R-HSA-629587|reactome:R-HSA-629594|reactome:R-HSA-629597|ensembl:ENSG00000101204(gene)|ensembl:ENST00000370263(transcript)|go:"GO:0001508"(action potential)|go:"GO:0001666"(response to hypoxia)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005892"(acetylcholine-gated channel complex)|go:"GO:0006281"(DNA repair)|go:"GO:0006811"(ion transport)|go:"GO:0006816"(calcium ion transport)|refseq:NP_001243502.1|refseq:NP_000735.1	ensembl:ENSG00000184164(gene)|ensembl:ENST00000328268(transcript)|intact:EBI-3935314(isoform-parent)	-	comment:mint|comment:homomint|function:After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane|function:"Defects in CHRNA4 are the cause of autosomal dominant nocturnal frontal lobe epilepsy type 1 (ENFL1) [MIM:600513]; also symbolized ADNFLE. ENFL1 is a disease characterized by clusters of motor seizures during sleep"	-	figure legend:Figure 3C|comment:"GenBank accession number NP_077300. (\"alpha\" isoform)  A weaker interaction than with CRELD2 - beta isoform."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	-	2019/04/09	2019/04/23	rogid:1mIuzGVLlNQeGoCu/fI+lbDZir49606	rogid:MJtefM+QiJRIn/HGdEr6vEd4bB49606	rigid:6L8uQVxNSye/fY6JtHlmzQrUheM	false	sufficient binding region:331-381	sufficient binding region:26-321	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P43681	uniprotkb:Q6UXH1-3	intact:EBI-7132379|ensembl:ENSP00000359285|uniprotkb:Q4JGR7|intact:MINT-3015931|uniprotkb:Q4VAQ6|intact:MINT-4869480|uniprotkb:Q4VAQ5	intact:EBI-21348090	psi-mi:acha4_human(display_long)|uniprotkb:CHRNA4(gene name)|psi-mi:CHRNA4(display_short)|uniprotkb:NACRA4(gene name synonym)	psi-mi:q6uxh1-3(display_long)|uniprotkb:CRELD2(gene name)|psi-mi:CRELD2(display_short)|uniprotkb:Cysteine-rich with EGF-like domain protein 2(gene name synonym)|uniprotkb:UNQ185/PRO211(orf name)|uniprotkb:Beta(isoform synonym)	psi-mi:"MI:0727"(lexa b52 complementation)	Ortiz et al. (2005)	pubmed:16238698|imex:IM-26962	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-21348126|imex:IM-26962-7	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:XP_011526826.1|go:"GO:0006979"(response to oxidative stress)|go:"GO:0007165"(signal transduction)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007271"(synaptic transmission, cholinergic)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0014059"(regulation of dopamine secretion)|go:"GO:0015276"(ligand-gated ion channel activity)|go:"GO:0015464"(acetylcholine receptor activity)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0030425"(dendrite)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0035094"(response to nicotine)|go:"GO:0035095"(behavioral response to nicotine)|go:"GO:0042113"(B cell activation)|go:"GO:0042166"(acetylcholine binding)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0043005"(neuron projection)|go:"GO:0043025"(neuronal cell body)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0050877"(nervous system process)|go:"GO:0050890"(cognition)|go:"GO:0051899"(membrane depolarization)|go:"GO:0060080"(inhibitory postsynaptic potential)|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR018000|interpro:IPR036719|interpro:IPR036734|rcsb pdb:2GVT|rcsb pdb:2LLY|rcsb pdb:5KXI|rcsb pdb:6CNJ|rcsb pdb:6CNK|rcsb pdb:6UR8|rcsb pdb:6USF|reactome:R-HSA-629587|reactome:R-HSA-629594|reactome:R-HSA-629597|ensembl:ENSG00000101204(gene)|ensembl:ENST00000370263(transcript)|go:"GO:0001508"(action potential)|go:"GO:0001666"(response to hypoxia)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005892"(acetylcholine-gated channel complex)|go:"GO:0006281"(DNA repair)|go:"GO:0006811"(ion transport)|go:"GO:0006816"(calcium ion transport)|refseq:NP_001243502.1|refseq:NP_000735.1	intact:EBI-3935314(isoform-parent)	-	comment:mint|comment:homomint|function:After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane|function:"Defects in CHRNA4 are the cause of autosomal dominant nocturnal frontal lobe epilepsy type 1 (ENFL1) [MIM:600513]; also symbolized ADNFLE. ENFL1 is a disease characterized by clusters of motor seizures during sleep"	isoform-comment:Minor isoform.	figure legend:Figure 3A/B/C|comment:"Clone B260, \"beta\" isoform. (Genbank identifier: AJ968414)"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	-	2019/04/09	2019/04/23	rogid:1mIuzGVLlNQeGoCu/fI+lbDZir49606	rogid:Pct6qcuvej1ixuinF8phSdFhLis9606	rigid:kj5mPEAUJkopVis/ZL6b1vfeVAE	false	sufficient binding region:331-381	sufficient binding region:26-284	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q6UXH1-3	uniprotkb:P17787	intact:EBI-21348090	intact:EBI-9008612|uniprotkb:Q9UEH9|ensembl:ENSP00000357461	psi-mi:q6uxh1-3(display_long)|uniprotkb:CRELD2(gene name)|psi-mi:CRELD2(display_short)|uniprotkb:Cysteine-rich with EGF-like domain protein 2(gene name synonym)|uniprotkb:UNQ185/PRO211(orf name)|uniprotkb:Beta(isoform synonym)	psi-mi:achb2_human(display_long)|uniprotkb:CHRNB2(gene name)|psi-mi:CHRNB2(display_short)	psi-mi:"MI:0727"(lexa b52 complementation)	Ortiz et al. (2005)	pubmed:16238698|imex:IM-26962	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-21348161|imex:IM-26962-9	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	intact:EBI-3935314(isoform-parent)	refseq:NP_000739.1|ensembl:ENSG00000160716(gene)|ensembl:ENST00000368476(transcript)|go:"GO:0001666"(response to hypoxia)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005892"(acetylcholine-gated channel complex)|go:"GO:0006811"(ion transport)|go:"GO:0006816"(calcium ion transport)|go:"GO:0006939"(smooth muscle contraction)|go:"GO:0007165"(signal transduction)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007271"(synaptic transmission, cholinergic)|go:"GO:0007601"(visual perception)|go:"GO:0007605"(sensory perception of sound)|go:"GO:0007612"(learning)|go:"GO:0007613"(memory)|go:"GO:0007626"(locomotory behavior)|go:"GO:0008306"(associative learning)|go:"GO:0008542"(visual learning)|go:"GO:0014059"(regulation of dopamine secretion)|go:"GO:0015276"(ligand-gated ion channel activity)|go:"GO:0015464"(acetylcholine receptor activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0021562"(vestibulocochlear nerve development)|go:"GO:0021631"(optic nerve morphogenesis)|go:"GO:0021771"(lateral geniculate nucleus development)|go:"GO:0021952"(central nervous system projection neuron axonogenesis)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0032225"(regulation of synaptic transmission, dopaminergic)|go:"GO:0033603"(positive regulation of dopamine secretion)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0035094"(response to nicotine)|go:"GO:0035095"(behavioral response to nicotine)|go:"GO:0035176"(social behavior)|go:"GO:0042053"(regulation of dopamine metabolic process)|go:"GO:0042113"(B cell activation)|go:"GO:0042166"(acetylcholine binding)|go:"GO:0042220"(response to cocaine)|go:"GO:0042320"(regulation of circadian sleep/wake cycle, REM sleep)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0043005"(neuron projection)|go:"GO:0044853"(plasma membrane raft)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045202"(synapse)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0048814"(regulation of dendrite morphogenesis)|go:"GO:0050877"(nervous system process)|go:"GO:0050890"(cognition)|go:"GO:0051899"(membrane depolarization)|go:"GO:0051963"(regulation of synapse assembly)|go:"GO:0060084"(synaptic transmission involved in micturition)|go:"GO:0095500"(acetylcholine receptor signaling pathway)|go:"GO:0098981"(cholinergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|go:"GO:0099060"(integral component of postsynaptic specialization membrane)|go:"GO:1905144"(response to acetylcholine)|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR018000|interpro:IPR032932|interpro:IPR036719|interpro:IPR036734|rcsb pdb:2GVT|go:"GO:0009897"(external side of plasma membrane)|rcsb pdb:2K58|rcsb pdb:2K59|rcsb pdb:2KSR|rcsb pdb:2LM2|rcsb pdb:5KXI|rcsb pdb:6CNJ|rcsb pdb:6CNK|rcsb pdb:6UR8|rcsb pdb:6USF|reactome:R-HSA-629587|reactome:R-HSA-629594|reactome:R-HSA-629597	-	isoform-comment:Minor isoform.	-	figure legend:Figure 3B|comment:"Clone B260, \"beta\" isoform. (Genbank identifier: AJ968414)"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	-	2019/04/09	2019/04/23	rogid:Pct6qcuvej1ixuinF8phSdFhLis9606	rogid:Ru/U+9R/PxJ2AZ3QQJ7fsoFhX9c9606	rigid:ki1LFoDvjBTKSHJIGs6Z4R/6fNI	false	sufficient binding region:26-284	sufficient binding region:322-460	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P43681	uniprotkb:Q6UXH1-1	intact:EBI-7132379|ensembl:ENSP00000359285|uniprotkb:Q4JGR7|intact:MINT-3015931|uniprotkb:Q4VAQ6|intact:MINT-4869480|uniprotkb:Q4VAQ5	intact:EBI-21348071|ensembl:ENSP00000332223	psi-mi:acha4_human(display_long)|uniprotkb:CHRNA4(gene name)|psi-mi:CHRNA4(display_short)|uniprotkb:NACRA4(gene name synonym)	psi-mi:q6uxh1-1(display_long)|uniprotkb:CRELD2(gene name)|psi-mi:CRELD2(display_short)|uniprotkb:Cysteine-rich with EGF-like domain protein 2(gene name synonym)|uniprotkb:UNQ185/PRO211(orf name)|uniprotkb:Delta(isoform synonym)	psi-mi:"MI:0096"(pull down)	Ortiz et al. (2005)	pubmed:16238698|imex:IM-26962	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-21348300|imex:IM-26962-11	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:XP_011526826.1|go:"GO:0006979"(response to oxidative stress)|go:"GO:0007165"(signal transduction)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007271"(synaptic transmission, cholinergic)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0014059"(regulation of dopamine secretion)|go:"GO:0015276"(ligand-gated ion channel activity)|go:"GO:0015464"(acetylcholine receptor activity)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0030425"(dendrite)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0035094"(response to nicotine)|go:"GO:0035095"(behavioral response to nicotine)|go:"GO:0042113"(B cell activation)|go:"GO:0042166"(acetylcholine binding)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0043005"(neuron projection)|go:"GO:0043025"(neuronal cell body)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0050877"(nervous system process)|go:"GO:0050890"(cognition)|go:"GO:0051899"(membrane depolarization)|go:"GO:0060080"(inhibitory postsynaptic potential)|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR018000|interpro:IPR036719|interpro:IPR036734|rcsb pdb:2GVT|rcsb pdb:2LLY|rcsb pdb:5KXI|rcsb pdb:6CNJ|rcsb pdb:6CNK|rcsb pdb:6UR8|rcsb pdb:6USF|reactome:R-HSA-629587|reactome:R-HSA-629594|reactome:R-HSA-629597|ensembl:ENSG00000101204(gene)|ensembl:ENST00000370263(transcript)|go:"GO:0001508"(action potential)|go:"GO:0001666"(response to hypoxia)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005892"(acetylcholine-gated channel complex)|go:"GO:0006281"(DNA repair)|go:"GO:0006811"(ion transport)|go:"GO:0006816"(calcium ion transport)|refseq:NP_001243502.1|refseq:NP_000735.1	ensembl:ENSG00000184164(gene)|ensembl:ENST00000328268(transcript)|intact:EBI-3935314(isoform-parent)	-	comment:mint|comment:homomint|function:After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane|function:"Defects in CHRNA4 are the cause of autosomal dominant nocturnal frontal lobe epilepsy type 1 (ENFL1) [MIM:600513]; also symbolized ADNFLE. ENFL1 is a disease characterized by clusters of motor seizures during sleep"	-	figure legend:Figure 4|comment:"GenBank accession number NP_077300. (\"alpha\" isoform)"|comment:"The N-terminal domain of CRELD2 (residues 1–130), common to both a and b isoforms, showed a much weaker interaction with the a4 cytoplasmic loop than the whole protein. A similar result was obtained using CRELD2 C-terminal regions."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2019/04/09	2019/04/24	rogid:1mIuzGVLlNQeGoCu/fI+lbDZir49606	rogid:MJtefM+QiJRIn/HGdEr6vEd4bB49606	rigid:6L8uQVxNSye/fY6JtHlmzQrUheM	false	sufficient binding region:331-600|glutathione s tranferase tag:n-n	35s radiolabel:?-?	-	-	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)
uniprotkb:P43681	uniprotkb:Q6UXH1-3	intact:EBI-7132379|ensembl:ENSP00000359285|uniprotkb:Q4JGR7|intact:MINT-3015931|uniprotkb:Q4VAQ6|intact:MINT-4869480|uniprotkb:Q4VAQ5	intact:EBI-21348090	psi-mi:acha4_human(display_long)|uniprotkb:CHRNA4(gene name)|psi-mi:CHRNA4(display_short)|uniprotkb:NACRA4(gene name synonym)	psi-mi:q6uxh1-3(display_long)|uniprotkb:CRELD2(gene name)|psi-mi:CRELD2(display_short)|uniprotkb:Cysteine-rich with EGF-like domain protein 2(gene name synonym)|uniprotkb:UNQ185/PRO211(orf name)|uniprotkb:Beta(isoform synonym)	psi-mi:"MI:0096"(pull down)	Ortiz et al. (2005)	pubmed:16238698|imex:IM-26962	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-21348309|imex:IM-26962-13	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:XP_011526826.1|go:"GO:0006979"(response to oxidative stress)|go:"GO:0007165"(signal transduction)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007271"(synaptic transmission, cholinergic)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0014059"(regulation of dopamine secretion)|go:"GO:0015276"(ligand-gated ion channel activity)|go:"GO:0015464"(acetylcholine receptor activity)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0030425"(dendrite)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0035094"(response to nicotine)|go:"GO:0035095"(behavioral response to nicotine)|go:"GO:0042113"(B cell activation)|go:"GO:0042166"(acetylcholine binding)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0043005"(neuron projection)|go:"GO:0043025"(neuronal cell body)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0050877"(nervous system process)|go:"GO:0050890"(cognition)|go:"GO:0051899"(membrane depolarization)|go:"GO:0060080"(inhibitory postsynaptic potential)|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR018000|interpro:IPR036719|interpro:IPR036734|rcsb pdb:2GVT|rcsb pdb:2LLY|rcsb pdb:5KXI|rcsb pdb:6CNJ|rcsb pdb:6CNK|rcsb pdb:6UR8|rcsb pdb:6USF|reactome:R-HSA-629587|reactome:R-HSA-629594|reactome:R-HSA-629597|ensembl:ENSG00000101204(gene)|ensembl:ENST00000370263(transcript)|go:"GO:0001508"(action potential)|go:"GO:0001666"(response to hypoxia)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005892"(acetylcholine-gated channel complex)|go:"GO:0006281"(DNA repair)|go:"GO:0006811"(ion transport)|go:"GO:0006816"(calcium ion transport)|refseq:NP_001243502.1|refseq:NP_000735.1	intact:EBI-3935314(isoform-parent)	-	comment:mint|comment:homomint|function:After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane|function:"Defects in CHRNA4 are the cause of autosomal dominant nocturnal frontal lobe epilepsy type 1 (ENFL1) [MIM:600513]; also symbolized ADNFLE. ENFL1 is a disease characterized by clusters of motor seizures during sleep"	isoform-comment:Minor isoform.	figure legend:Figure 4|comment:"Clone B260, \"beta\" isoform. (Genbank identifier: AJ968414)"|comment:"The N-terminal domain of CRELD2 (residues 1–130), common to both a and b isoforms, showed a much weaker interaction with the a4 cytoplasmic loop than the whole protein. A similar result was obtained using CRELD2 C-terminal regions."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2019/04/09	2019/04/24	rogid:1mIuzGVLlNQeGoCu/fI+lbDZir49606	rogid:Pct6qcuvej1ixuinF8phSdFhLis9606	rigid:kj5mPEAUJkopVis/ZL6b1vfeVAE	false	sufficient binding region:331-600|glutathione s tranferase tag:n-n	35s radiolabel:?-?	-	-	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)
uniprotkb:Q6UXH1-3	uniprotkb:P17787	intact:EBI-21348090	intact:EBI-9008612|uniprotkb:Q9UEH9|ensembl:ENSP00000357461	psi-mi:q6uxh1-3(display_long)|uniprotkb:CRELD2(gene name)|psi-mi:CRELD2(display_short)|uniprotkb:Cysteine-rich with EGF-like domain protein 2(gene name synonym)|uniprotkb:UNQ185/PRO211(orf name)|uniprotkb:Beta(isoform synonym)	psi-mi:achb2_human(display_long)|uniprotkb:CHRNB2(gene name)|psi-mi:CHRNB2(display_short)	psi-mi:"MI:0663"(confocal microscopy)	Ortiz et al. (2005)	pubmed:16238698|imex:IM-26962	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0403"(colocalization)	psi-mi:"MI:0469"(IntAct)	intact:EBI-21348336|imex:IM-26962-15	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	intact:EBI-3935314(isoform-parent)	refseq:NP_000739.1|ensembl:ENSG00000160716(gene)|ensembl:ENST00000368476(transcript)|go:"GO:0001666"(response to hypoxia)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005892"(acetylcholine-gated channel complex)|go:"GO:0006811"(ion transport)|go:"GO:0006816"(calcium ion transport)|go:"GO:0006939"(smooth muscle contraction)|go:"GO:0007165"(signal transduction)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007271"(synaptic transmission, cholinergic)|go:"GO:0007601"(visual perception)|go:"GO:0007605"(sensory perception of sound)|go:"GO:0007612"(learning)|go:"GO:0007613"(memory)|go:"GO:0007626"(locomotory behavior)|go:"GO:0008306"(associative learning)|go:"GO:0008542"(visual learning)|go:"GO:0014059"(regulation of dopamine secretion)|go:"GO:0015276"(ligand-gated ion channel activity)|go:"GO:0015464"(acetylcholine receptor activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0021562"(vestibulocochlear nerve development)|go:"GO:0021631"(optic nerve morphogenesis)|go:"GO:0021771"(lateral geniculate nucleus development)|go:"GO:0021952"(central nervous system projection neuron axonogenesis)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0032225"(regulation of synaptic transmission, dopaminergic)|go:"GO:0033603"(positive regulation of dopamine secretion)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0035094"(response to nicotine)|go:"GO:0035095"(behavioral response to nicotine)|go:"GO:0035176"(social behavior)|go:"GO:0042053"(regulation of dopamine metabolic process)|go:"GO:0042113"(B cell activation)|go:"GO:0042166"(acetylcholine binding)|go:"GO:0042220"(response to cocaine)|go:"GO:0042320"(regulation of circadian sleep/wake cycle, REM sleep)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0043005"(neuron projection)|go:"GO:0044853"(plasma membrane raft)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045202"(synapse)|go:"GO:0045471"(response to ethanol)|go:"GO:0045759"(negative regulation of action potential)|go:"GO:0048814"(regulation of dendrite morphogenesis)|go:"GO:0050877"(nervous system process)|go:"GO:0050890"(cognition)|go:"GO:0051899"(membrane depolarization)|go:"GO:0051963"(regulation of synapse assembly)|go:"GO:0060084"(synaptic transmission involved in micturition)|go:"GO:0095500"(acetylcholine receptor signaling pathway)|go:"GO:0098981"(cholinergic synapse)|go:"GO:0099056"(integral component of presynaptic membrane)|go:"GO:0099060"(integral component of postsynaptic specialization membrane)|go:"GO:1905144"(response to acetylcholine)|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR018000|interpro:IPR032932|interpro:IPR036719|interpro:IPR036734|rcsb pdb:2GVT|go:"GO:0009897"(external side of plasma membrane)|rcsb pdb:2K58|rcsb pdb:2K59|rcsb pdb:2KSR|rcsb pdb:2LM2|rcsb pdb:5KXI|rcsb pdb:6CNJ|rcsb pdb:6CNK|rcsb pdb:6UR8|rcsb pdb:6USF|reactome:R-HSA-629587|reactome:R-HSA-629594|reactome:R-HSA-629597	go:"GO:0005783"(endoplasmic reticulum)	isoform-comment:Minor isoform.	-	figure legend:Figure 6|comment:"Untagged alpha-4 nAChR (CHRNA4;Uniprot ID = P43681)  was also transfected."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9534(chlae-vero)|taxid:9534("Chlorocebus (Cercopithecus) aethiops kidney cells")	-	2019/04/09	2019/04/24	rogid:Pct6qcuvej1ixuinF8phSdFhLis9606	rogid:Ru/U+9R/PxJ2AZ3QQJ7fsoFhX9c9606	rigid:ki1LFoDvjBTKSHJIGs6Z4R/6fNI	false	red fluorescent protein tag:?-?	green fluorescent protein tag:c-c	-	-	psi-mi:"MI:0867"(tag visualisation by fluorescence)	psi-mi:"MI:0867"(tag visualisation by fluorescence)