#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
ddbj/embl/genbank:M95666	uniprotkb:Q06253	intact:EBI-2908801	intact:EBI-2908787	psi-mi:ta_p1_operator(display_short)|psi-mi:M95666(display_long)	psi-mi:phd_bpp1(display_long)|uniprotkb:phd(gene name)|psi-mi:phd(display_short)|uniprotkb:Addiction protein pdh(gene name synonym)|uniprotkb:Prevent host death protein(gene name synonym)	psi-mi:"MI:0016"(circular dichroism)	Garcia-Pino et al. (2010)	imex:IM-14972|pubmed:20603017	taxid:10678(bpp1)|taxid:10678(Enterobacteria phage P1)	taxid:10678(bpp1)|taxid:10678(Enterobacteria phage P1)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2908797|imex:IM-14972-7	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0319"(deoxyribonucleic acid)	psi-mi:"MI:0326"(protein)	-	go:"GO:0032993"(protein-DNA complex)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0043565"(sequence-specific DNA binding)|interpro:IPR006442(Prevent-host-death protein)|interpro:IPR036165|rcsb pdb:3DD7|rcsb pdb:3HRY|rcsb pdb:3HS2|rcsb pdb:3K33|rcsb pdb:3KH2|rcsb pdb:4ZLX|rcsb pdb:4ZM0|rcsb pdb:4ZM2|refseq:YP_006570.1|dip:DIP-62083N	-	comment:"Contains two phd binding sites: OR1 consisting of a perfect palindrome (5'-TGTGTACACA-3' and OR2 consisting of an imperfect palindrome (5'-TGAGTACACA-3')."	-	figure legend:Fig 2A.|comment:An alpha helix is induced in Phd upon addition of operator DNA in vitro.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2010/08/17	2014/10/16	-	rogid:pYz+SCI+RweXyW9txckzCjdtA5Q10678	intact-crc:EB9E204E1331FFF5	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q06259	uniprotkb:Q06253	intact:EBI-2908816	intact:EBI-2908787	psi-mi:doc_bpp1(display_long)|uniprotkb:doc(gene name)|psi-mi:doc(display_short)|uniprotkb:Death on curing protein(gene name synonym)|uniprotkb:Toxin doc(gene name synonym)	psi-mi:phd_bpp1(display_long)|uniprotkb:phd(gene name)|psi-mi:phd(display_short)|uniprotkb:Addiction protein pdh(gene name synonym)|uniprotkb:Prevent host death protein(gene name synonym)	psi-mi:"MI:0107"(surface plasmon resonance)	Garcia-Pino et al. (2010)	imex:IM-14972|pubmed:20603017	taxid:10678(bpp1)|taxid:10678(Enterobacteria phage P1)	taxid:10678(bpp1)|taxid:10678(Enterobacteria phage P1)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2908812|imex:IM-14972-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0003677"(DNA binding)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0039604"(suppression by virus of host translation)|go:"GO:0044074"(negative regulation by symbiont of host translation)|go:"GO:0044696"(killing by virus of host cell by post-segregational killing)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR003812(Filamentation induced by cAMP protein Fic)|interpro:IPR006440(Death-on-curing protein)|rcsb pdb:3DD9|rcsb pdb:3K33|rcsb pdb:3KH2|rcsb pdb:3DD7|refseq:YP_006571.1|dip:DIP-61743N	go:"GO:0032993"(protein-DNA complex)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0043565"(sequence-specific DNA binding)|interpro:IPR006442(Prevent-host-death protein)|interpro:IPR036165|rcsb pdb:3DD7|rcsb pdb:3HRY|rcsb pdb:3HS2|rcsb pdb:3K33|rcsb pdb:3KH2|rcsb pdb:4ZLX|rcsb pdb:4ZM0|rcsb pdb:4ZM2|refseq:YP_006570.1|dip:DIP-62083N	psi-mi:"MI:0469"(intact)	-	-	figure legend:Fig 2C, Table S4.|kinetics:The best model that explains the binding data involves two Phd dimers binding to a Doc monomer with affinities of 0.35 uM and 31 uM.|kinetic_conditions:25 degrees C in 20 mM HEPES pH 7.5.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2010/08/17	2014/10/16	rogid:RqRmxYsC/026Cu2KVmktQ6oJZc810678	rogid:pYz+SCI+RweXyW9txckzCjdtA5Q10678	intact-crc:8FCEAE3835D132E4|rigid:kztXHhQI70UNuMCI4DNuN4MITvs	false	-	-	1	2	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q06259	uniprotkb:Q06253	intact:EBI-2908816	intact:EBI-2908787	psi-mi:doc_bpp1(display_long)|uniprotkb:doc(gene name)|psi-mi:doc(display_short)|uniprotkb:Death on curing protein(gene name synonym)|uniprotkb:Toxin doc(gene name synonym)	psi-mi:phd_bpp1(display_long)|uniprotkb:phd(gene name)|psi-mi:phd(display_short)|uniprotkb:Addiction protein pdh(gene name synonym)|uniprotkb:Prevent host death protein(gene name synonym)	psi-mi:"MI:0065"(isothermal titration calorimetry)	Garcia-Pino et al. (2010)	imex:IM-14972|pubmed:20603017	taxid:10678(bpp1)|taxid:10678(Enterobacteria phage P1)	taxid:10678(bpp1)|taxid:10678(Enterobacteria phage P1)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2908916|imex:IM-14972-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0003677"(DNA binding)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0039604"(suppression by virus of host translation)|go:"GO:0044074"(negative regulation by symbiont of host translation)|go:"GO:0044696"(killing by virus of host cell by post-segregational killing)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR003812(Filamentation induced by cAMP protein Fic)|interpro:IPR006440(Death-on-curing protein)|rcsb pdb:3DD9|rcsb pdb:3K33|rcsb pdb:3KH2|rcsb pdb:3DD7|refseq:YP_006571.1|dip:DIP-61743N	go:"GO:0032993"(protein-DNA complex)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0043565"(sequence-specific DNA binding)|interpro:IPR006442(Prevent-host-death protein)|interpro:IPR036165|rcsb pdb:3DD7|rcsb pdb:3HRY|rcsb pdb:3HS2|rcsb pdb:3K33|rcsb pdb:3KH2|rcsb pdb:4ZLX|rcsb pdb:4ZM0|rcsb pdb:4ZM2|refseq:YP_006570.1|dip:DIP-62083N	psi-mi:"MI:0469"(intact)	-	-	figure legend:Fig 2D, Table S4.|kinetic_conditions:Temp=25C, pH 7.5.|kinetics:For mutations DocH66Y/A61R, Kd = 0.29 uM, DocH66Y/N78W Kd = 43.9 uM.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2010/08/17	2014/10/16	rogid:RqRmxYsC/026Cu2KVmktQ6oJZc810678	rogid:pYz+SCI+RweXyW9txckzCjdtA5Q10678	intact-crc:28C53E3B70E00E7B|rigid:kztXHhQI70UNuMCI4DNuN4MITvs	false	-	sufficient binding region:51-73	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
ddbj/embl/genbank:M95666	uniprotkb:Q06253	intact:EBI-2908801	intact:EBI-2908787	psi-mi:ta_p1_operator(display_short)|psi-mi:M95666(display_long)	psi-mi:phd_bpp1(display_long)|uniprotkb:phd(gene name)|psi-mi:phd(display_short)|uniprotkb:Addiction protein pdh(gene name synonym)|uniprotkb:Prevent host death protein(gene name synonym)	psi-mi:"MI:0065"(isothermal titration calorimetry)	Garcia-Pino et al. (2010)	imex:IM-14972|pubmed:20603017	taxid:10678(bpp1)|taxid:10678(Enterobacteria phage P1)	taxid:10678(bpp1)|taxid:10678(Enterobacteria phage P1)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2909100|imex:IM-14972-9	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0319"(deoxyribonucleic acid)	psi-mi:"MI:0326"(protein)	-	go:"GO:0032993"(protein-DNA complex)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0043565"(sequence-specific DNA binding)|interpro:IPR006442(Prevent-host-death protein)|interpro:IPR036165|rcsb pdb:3DD7|rcsb pdb:3HRY|rcsb pdb:3HS2|rcsb pdb:3K33|rcsb pdb:3KH2|rcsb pdb:4ZLX|rcsb pdb:4ZM0|rcsb pdb:4ZM2|refseq:YP_006570.1|dip:DIP-62083N	-	comment:"Contains two phd binding sites: OR1 consisting of a perfect palindrome (5'-TGTGTACACA-3' and OR2 consisting of an imperfect palindrome (5'-TGAGTACACA-3')."	-	figure legend:Fig 3D, Table S4.|kinetic_conditions:Temp=25C, pH 7.5.|comment:"Binding of Phd to OR1 (a single palindromic sequence (5'-TGTGTACACA-3'))from the phd/doc operon operator."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2010/08/17	2014/10/16	-	rogid:pYz+SCI+RweXyW9txckzCjdtA5Q10678	intact-crc:A89708D202210DAF	false	-	sufficient binding region:51-73	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q06259	uniprotkb:Q06253	intact:EBI-2908816	intact:EBI-2908787	psi-mi:doc_bpp1(display_long)|uniprotkb:doc(gene name)|psi-mi:doc(display_short)|uniprotkb:Death on curing protein(gene name synonym)|uniprotkb:Toxin doc(gene name synonym)	psi-mi:phd_bpp1(display_long)|uniprotkb:phd(gene name)|psi-mi:phd(display_short)|uniprotkb:Addiction protein pdh(gene name synonym)|uniprotkb:Prevent host death protein(gene name synonym)	psi-mi:"MI:0065"(isothermal titration calorimetry)	Garcia-Pino et al. (2010)	imex:IM-14972|pubmed:20603017	taxid:10678(bpp1)|taxid:10678(Enterobacteria phage P1)	taxid:10678(bpp1)|taxid:10678(Enterobacteria phage P1)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2909109|imex:IM-14972-6	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0003677"(DNA binding)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0039604"(suppression by virus of host translation)|go:"GO:0044074"(negative regulation by symbiont of host translation)|go:"GO:0044696"(killing by virus of host cell by post-segregational killing)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR003812(Filamentation induced by cAMP protein Fic)|interpro:IPR006440(Death-on-curing protein)|rcsb pdb:3DD9|rcsb pdb:3K33|rcsb pdb:3KH2|rcsb pdb:3DD7|refseq:YP_006571.1|dip:DIP-61743N	go:"GO:0032993"(protein-DNA complex)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0043565"(sequence-specific DNA binding)|interpro:IPR006442(Prevent-host-death protein)|interpro:IPR036165|rcsb pdb:3DD7|rcsb pdb:3HRY|rcsb pdb:3HS2|rcsb pdb:3K33|rcsb pdb:3KH2|rcsb pdb:4ZLX|rcsb pdb:4ZM0|rcsb pdb:4ZM2|refseq:YP_006570.1|dip:DIP-62083N	-	-	-	figure legend:Fig 3E, Table S4.|kinetic_conditions:Temp=25C, pH 7.5.|comment:Doc-mediated enhancement of Phd binding to operator DNA is observed in operator regions containing a single Phd-binding site;the affinity of Phd2/Doc for OR1 is 0.3 uM, about 10-fold higher than the binding constant of Phd2 for OR1.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2010/08/17	2014/10/16	rogid:RqRmxYsC/026Cu2KVmktQ6oJZc810678	rogid:pYz+SCI+RweXyW9txckzCjdtA5Q10678	intact-crc:CABC051808E56BE8	false	-	sufficient binding region:51-73	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q06259	ddbj/embl/genbank:M95666	intact:EBI-2908816	intact:EBI-2908801	psi-mi:doc_bpp1(display_long)|uniprotkb:doc(gene name)|psi-mi:doc(display_short)|uniprotkb:Death on curing protein(gene name synonym)|uniprotkb:Toxin doc(gene name synonym)	psi-mi:ta_p1_operator(display_short)|psi-mi:M95666(display_long)	psi-mi:"MI:0065"(isothermal titration calorimetry)	Garcia-Pino et al. (2010)	imex:IM-14972|pubmed:20603017	taxid:10678(bpp1)|taxid:10678(Enterobacteria phage P1)	taxid:10678(bpp1)|taxid:10678(Enterobacteria phage P1)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2909109|imex:IM-14972-6	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0319"(deoxyribonucleic acid)	go:"GO:0003677"(DNA binding)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0039604"(suppression by virus of host translation)|go:"GO:0044074"(negative regulation by symbiont of host translation)|go:"GO:0044696"(killing by virus of host cell by post-segregational killing)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR003812(Filamentation induced by cAMP protein Fic)|interpro:IPR006440(Death-on-curing protein)|rcsb pdb:3DD9|rcsb pdb:3K33|rcsb pdb:3KH2|rcsb pdb:3DD7|refseq:YP_006571.1|dip:DIP-61743N	-	-	-	comment:"Contains two phd binding sites: OR1 consisting of a perfect palindrome (5'-TGTGTACACA-3' and OR2 consisting of an imperfect palindrome (5'-TGAGTACACA-3')."	figure legend:Fig 3E, Table S4.|kinetic_conditions:Temp=25C, pH 7.5.|comment:Doc-mediated enhancement of Phd binding to operator DNA is observed in operator regions containing a single Phd-binding site;the affinity of Phd2/Doc for OR1 is 0.3 uM, about 10-fold higher than the binding constant of Phd2 for OR1.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2010/08/17	2014/10/16	rogid:RqRmxYsC/026Cu2KVmktQ6oJZc810678	-	intact-crc:CABC051808E56BE8	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q06253	ddbj/embl/genbank:M95666	intact:EBI-2908787	intact:EBI-2908801	psi-mi:phd_bpp1(display_long)|uniprotkb:phd(gene name)|psi-mi:phd(display_short)|uniprotkb:Addiction protein pdh(gene name synonym)|uniprotkb:Prevent host death protein(gene name synonym)	psi-mi:ta_p1_operator(display_short)|psi-mi:M95666(display_long)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Garcia-Pino et al. (2010)	imex:IM-14972|pubmed:20603017	taxid:10678(bpp1)|taxid:10678(Enterobacteria phage P1)	taxid:10678(bpp1)|taxid:10678(Enterobacteria phage P1)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2909065|imex:IM-14972-8	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0319"(deoxyribonucleic acid)	go:"GO:0032993"(protein-DNA complex)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0043565"(sequence-specific DNA binding)|interpro:IPR006442(Prevent-host-death protein)|interpro:IPR036165|rcsb pdb:3DD7|rcsb pdb:3HRY|rcsb pdb:3HS2|rcsb pdb:3K33|rcsb pdb:3KH2|rcsb pdb:4ZLX|rcsb pdb:4ZM0|rcsb pdb:4ZM2|refseq:YP_006570.1|dip:DIP-62083N	-	-	-	comment:"Contains two phd binding sites: OR1 consisting of a perfect palindrome (5'-TGTGTACACA-3' and OR2 consisting of an imperfect palindrome (5'-TGAGTACACA-3')."	figure legend:Fig 3A, S3A.|comment:"Wild-type Phd binding to OR1/2 (operator region of the phd/doc operon containing the two binding sites). The affinity of these mutants for OR1/2 is much weaker than that of the wild-type protein."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2010/08/17	2014/10/16	rogid:pYz+SCI+RweXyW9txckzCjdtA5Q10678	-	intact-crc:D01FF849A4FA17B1	false	mutation decreasing interaction:44-44|mutation decreasing interaction:47-47|mutation decreasing interaction:48-48	-	-	-	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)
ddbj/embl/genbank:M95666	uniprotkb:Q06259	intact:EBI-2908801	intact:EBI-2908816	psi-mi:ta_p1_operator(display_short)|psi-mi:M95666(display_long)	psi-mi:doc_bpp1(display_long)|uniprotkb:doc(gene name)|psi-mi:doc(display_short)|uniprotkb:Death on curing protein(gene name synonym)|uniprotkb:Toxin doc(gene name synonym)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Garcia-Pino et al. (2010)	imex:IM-14972|pubmed:20603017	taxid:10678(bpp1)|taxid:10678(Enterobacteria phage P1)	taxid:10678(bpp1)|taxid:10678(Enterobacteria phage P1)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2909072|imex:IM-14972-5	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0319"(deoxyribonucleic acid)	psi-mi:"MI:0326"(protein)	-	go:"GO:0003677"(DNA binding)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0039604"(suppression by virus of host translation)|go:"GO:0044074"(negative regulation by symbiont of host translation)|go:"GO:0044696"(killing by virus of host cell by post-segregational killing)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR003812(Filamentation induced by cAMP protein Fic)|interpro:IPR006440(Death-on-curing protein)|rcsb pdb:3DD9|rcsb pdb:3K33|rcsb pdb:3KH2|rcsb pdb:3DD7|refseq:YP_006571.1|dip:DIP-61743N	-	comment:"Contains two phd binding sites: OR1 consisting of a perfect palindrome (5'-TGTGTACACA-3' and OR2 consisting of an imperfect palindrome (5'-TGAGTACACA-3')."	-	figure legend:Fig 3B,3F, S3C, 5A.|comment:Compared to wild-type Phd, the mutants PhdF44A and PhdY47A show little if any increase in their affinity for the OR1/2 DNA in presence of Doc.  Phd mutants F44A and Y47A bind to Doc, but this does not lead to an increase of the affinity of the Phd mutant for OR1. Mutant K48M shows weak but observable enhancement of Phd2 binding to OR1 in the presence of Doc.  Doc mutants  H66Y/N78W, and H66Y/A61R show a less complex set of bands in EMSA.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2010/08/17	2014/10/16	-	rogid:RqRmxYsC/026Cu2KVmktQ6oJZc810678	intact-crc:9F89FDD2F2F1109E	false	-	-	-	-	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)
ddbj/embl/genbank:M95666	uniprotkb:Q06253	intact:EBI-2908801	intact:EBI-2908787	psi-mi:ta_p1_operator(display_short)|psi-mi:M95666(display_long)	psi-mi:phd_bpp1(display_long)|uniprotkb:phd(gene name)|psi-mi:phd(display_short)|uniprotkb:Addiction protein pdh(gene name synonym)|uniprotkb:Prevent host death protein(gene name synonym)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Garcia-Pino et al. (2010)	imex:IM-14972|pubmed:20603017	taxid:10678(bpp1)|taxid:10678(Enterobacteria phage P1)	taxid:10678(bpp1)|taxid:10678(Enterobacteria phage P1)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2909072|imex:IM-14972-5	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0319"(deoxyribonucleic acid)	psi-mi:"MI:0326"(protein)	-	go:"GO:0032993"(protein-DNA complex)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0043565"(sequence-specific DNA binding)|interpro:IPR006442(Prevent-host-death protein)|interpro:IPR036165|rcsb pdb:3DD7|rcsb pdb:3HRY|rcsb pdb:3HS2|rcsb pdb:3K33|rcsb pdb:3KH2|rcsb pdb:4ZLX|rcsb pdb:4ZM0|rcsb pdb:4ZM2|refseq:YP_006570.1|dip:DIP-62083N	-	comment:"Contains two phd binding sites: OR1 consisting of a perfect palindrome (5'-TGTGTACACA-3' and OR2 consisting of an imperfect palindrome (5'-TGAGTACACA-3')."	-	figure legend:Fig 3B,3F, S3C, 5A.|comment:Compared to wild-type Phd, the mutants PhdF44A and PhdY47A show little if any increase in their affinity for the OR1/2 DNA in presence of Doc.  Phd mutants F44A and Y47A bind to Doc, but this does not lead to an increase of the affinity of the Phd mutant for OR1. Mutant K48M shows weak but observable enhancement of Phd2 binding to OR1 in the presence of Doc.  Doc mutants  H66Y/N78W, and H66Y/A61R show a less complex set of bands in EMSA.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2010/08/17	2014/10/16	-	rogid:pYz+SCI+RweXyW9txckzCjdtA5Q10678	intact-crc:9F89FDD2F2F1109E	false	-	-	-	-	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)
uniprotkb:Q06259	uniprotkb:Q06253	intact:EBI-2908816	intact:EBI-2908787	psi-mi:doc_bpp1(display_long)|uniprotkb:doc(gene name)|psi-mi:doc(display_short)|uniprotkb:Death on curing protein(gene name synonym)|uniprotkb:Toxin doc(gene name synonym)	psi-mi:phd_bpp1(display_long)|uniprotkb:phd(gene name)|psi-mi:phd(display_short)|uniprotkb:Addiction protein pdh(gene name synonym)|uniprotkb:Prevent host death protein(gene name synonym)	psi-mi:"MI:0114"(x-ray crystallography)	Garcia-Pino et al. (2010)	imex:IM-14972|pubmed:20603017	taxid:10678(bpp1)|taxid:10678(Enterobacteria phage P1)	taxid:10678(bpp1)|taxid:10678(Enterobacteria phage P1)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2909172|wwpdb:3k33|imex:IM-14972-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0003677"(DNA binding)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0039604"(suppression by virus of host translation)|go:"GO:0044074"(negative regulation by symbiont of host translation)|go:"GO:0044696"(killing by virus of host cell by post-segregational killing)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR003812(Filamentation induced by cAMP protein Fic)|interpro:IPR006440(Death-on-curing protein)|rcsb pdb:3DD9|rcsb pdb:3K33|rcsb pdb:3KH2|rcsb pdb:3DD7|refseq:YP_006571.1|dip:DIP-61743N	go:"GO:0032993"(protein-DNA complex)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0043565"(sequence-specific DNA binding)|interpro:IPR006442(Prevent-host-death protein)|interpro:IPR036165|rcsb pdb:3DD7|rcsb pdb:3HRY|rcsb pdb:3HS2|rcsb pdb:3K33|rcsb pdb:3KH2|rcsb pdb:4ZLX|rcsb pdb:4ZM0|rcsb pdb:4ZM2|refseq:YP_006570.1|dip:DIP-62083N	-	-	-	figure legend:Fig 4, Fig S4, Table 1.|3d-r-factors:working 25.7%, free 28.5%|3d-resolution:2.40 A|3d-structure:The asymmetric unit of the Phd/Doc cocrystals contain three copies of Phd and one copy of Doc, which through crystal symmetry form a hetero-octameric Phd2-Doc-Phd2-Doc-Phd2 complex.|comment:"Doc Site H (encompassing residues from alpha helices a1, a4, a5, and the loop a4-a5 of Doc) binds to Phd segment Phe56-Arg73.   Doc Site L  (mainly encompassing residues from loops  a1-a2 (residues 28�31) and a5-a6 (residues106�111) and from helices a2 (34�35, 38�39, 42) and a3 (57�61, 64�65)), binds to Leu52-Thr62 of Phd."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2010/08/17	2014/10/16	rogid:RqRmxYsC/026Cu2KVmktQ6oJZc810678	rogid:pYz+SCI+RweXyW9txckzCjdtA5Q10678	intact-crc:A37F02F9F4F70281|rigid:kztXHhQI70UNuMCI4DNuN4MITvs	false	-	-	1	3	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q06253	uniprotkb:Q06253	intact:EBI-2908787	intact:EBI-2908787	psi-mi:phd_bpp1(display_long)|uniprotkb:phd(gene name)|psi-mi:phd(display_short)|uniprotkb:Addiction protein pdh(gene name synonym)|uniprotkb:Prevent host death protein(gene name synonym)	psi-mi:phd_bpp1(display_long)|uniprotkb:phd(gene name)|psi-mi:phd(display_short)|uniprotkb:Addiction protein pdh(gene name synonym)|uniprotkb:Prevent host death protein(gene name synonym)	psi-mi:"MI:0114"(x-ray crystallography)	Garcia-Pino et al. (2010)	imex:IM-14972|pubmed:20603017	taxid:10678(bpp1)|taxid:10678(Enterobacteria phage P1)	taxid:10678(bpp1)|taxid:10678(Enterobacteria phage P1)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2911730|wwpdb:3HS2|imex:IM-14972-4	-	-	psi-mi:"MI:0840"(stimulator)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0032993"(protein-DNA complex)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0043565"(sequence-specific DNA binding)|interpro:IPR006442(Prevent-host-death protein)|interpro:IPR036165|rcsb pdb:3DD7|rcsb pdb:3HRY|rcsb pdb:3HS2|rcsb pdb:3K33|rcsb pdb:3KH2|rcsb pdb:4ZLX|rcsb pdb:4ZM0|rcsb pdb:4ZM2|refseq:YP_006570.1|dip:DIP-62083N	go:"GO:0032993"(protein-DNA complex)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0043565"(sequence-specific DNA binding)|interpro:IPR006442(Prevent-host-death protein)|interpro:IPR036165|rcsb pdb:3DD7|rcsb pdb:3HRY|rcsb pdb:3HS2|rcsb pdb:3K33|rcsb pdb:3KH2|rcsb pdb:4ZLX|rcsb pdb:4ZM0|rcsb pdb:4ZM2|refseq:YP_006570.1|dip:DIP-62083N	-	-	-	figure legend:Fig 1A.|3d-r-factors:working 23%, free 27.1%|3d-resolution:2.20 A|3d-structure:"This N-terminal domain forms a small globular homodimer comprising a central six-stranded beta sheet decorated with four alpha helices (two from each monomer), resembling the antitoxin YefM (Kamada and Hanaoka, 2005)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2010/08/17	2014/10/16	rogid:pYz+SCI+RweXyW9txckzCjdtA5Q10678	rogid:pYz+SCI+RweXyW9txckzCjdtA5Q10678	intact-crc:4C55A9F3FC4EF2F4|rigid:K3rDE0IXtWiWk5LlOzYfTJX0IqE	false	sufficient binding region:1-57	sufficient binding region:1-57	1	1	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)