#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q92888	uniprotkb:Q14344	intact:EBI-465400|uniprotkb:O00513|uniprotkb:Q8N4J4|uniprotkb:Q96BF4|uniprotkb:Q96F17|uniprotkb:Q9BSB1|uniprotkb:Q6NX52|ensembl:ENSP00000346532	intact:EBI-465387|ensembl:ENSP00000400717|uniprotkb:Q8TD70|uniprotkb:B7Z7R0|uniprotkb:F5H1G8|uniprotkb:B2R977	psi-mi:arhg1_human(display_long)|uniprotkb:ARHGEF1(gene name)|psi-mi:ARHGEF1(display_short)|uniprotkb:115 kDa guanine nucleotide exchange factor(gene name synonym)|uniprotkb:Sub1.5(gene name synonym)	psi-mi:gna13_human(display_long)|uniprotkb:GNA13(gene name)|psi-mi:GNA13(display_short)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Bhattacharyya et al. (2003)	pubmed:12681510	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-465428	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_004697.2|refseq:NP_945328.1|refseq:NP_945353.1|reactome:R-HSA-193648|reactome:R-HSA-416482|reactome:R-HSA-8980692|reactome:R-HSA-9013026|reactome:R-HSA-9013106|ensembl:ENSG00000076928(gene)|ensembl:ENST00000354532(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003723"(RNA binding)|go:"GO:0005085"(guanyl-nucleotide exchange factor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007266"(Rho protein signal transduction)|go:"GO:0051056"(regulation of small GTPase mediated signal transduction)|interpro:IPR000219("Dbl homology (DH) domain")|interpro:IPR001849(Pleckstrin-like)|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR015212(Regulator of G protein signalling-like fold)|interpro:IPR035899|interpro:IPR036305|interpro:IPR037887|interpro:IPR041020|mint:Q92888|rcsb pdb:1IAP|rcsb pdb:1SHZ|rcsb pdb:3AB3|rcsb pdb:3ODO|rcsb pdb:3ODW|rcsb pdb:3ODX|rcsb pdb:3P6A	ensembl:ENSG00000120063(gene)|ensembl:ENST00000439174(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003924"(GTPase activity)|go:"GO:0005085"(guanyl-nucleotide exchange factor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0007165"(signal transduction)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007204"(positive regulation of cytosolic calcium ion concentration)|go:"GO:0007266"(Rho protein signal transduction)|go:"GO:0016020"(membrane)|go:"GO:0031526"(brush border membrane)|go:"GO:0031584"(activation of phospholipase D activity)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0031752"(D5 dopamine receptor binding)|go:"GO:0042470"(melanosome)|go:"GO:0046872"(metal ion binding)|go:"GO:0051056"(regulation of small GTPase mediated signal transduction)|go:"GO:0070062"(extracellular exosome)|go:"GO:1904706"(negative regulation of vascular associated smooth muscle cell proliferation)|go:"GO:1904753"(negative regulation of vascular associated smooth muscle cell migration)|interpro:IPR000469(G-protein alpha subunit, group 12)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|reactome:R-HSA-193648|reactome:R-HSA-416482|reactome:R-HSA-428930|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|reactome:R-HSA-9013148|reactome:R-HSA-9013149|reactome:R-HSA-456926|refseq:NP_006563.2|refseq:NP_001269354.1	-	-	-	stimulant:AlF4- is added to stabilize the complex between GAP/G protein, here it enables to detect the P115/Galpha13 interaction. Same experiments were conducted without AlF4-, but with the GTPase-deficient G alpha 13 mutant Gln226Leu.	taxid:9534(chlae-cos_7)|taxid:9534(Cercopithecus aethiops simian cells transformed with SV40)	-	2004/12/06	2014/10/16	rogid:XIZmflM2cr80ZBD3FO21IDMnInE9606	rogid:ZYmlpIogjya3/ejteQS1YC+LqKI9606	intact-crc:A8E85C8610A0BA3C|rigid:+A00WVKkm/4W6VEBfgEpo2Tqgxo	false	myc tag:n-n|necessary binding region:22-31|mutation decreasing interaction:29-29,27-27	ha tag:n-n	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q92888	uniprotkb:Q14344	intact:EBI-465400|uniprotkb:O00513|uniprotkb:Q8N4J4|uniprotkb:Q96BF4|uniprotkb:Q96F17|uniprotkb:Q9BSB1|uniprotkb:Q6NX52|ensembl:ENSP00000346532	intact:EBI-465387|ensembl:ENSP00000400717|uniprotkb:Q8TD70|uniprotkb:B7Z7R0|uniprotkb:F5H1G8|uniprotkb:B2R977	psi-mi:arhg1_human(display_long)|uniprotkb:ARHGEF1(gene name)|psi-mi:ARHGEF1(display_short)|uniprotkb:115 kDa guanine nucleotide exchange factor(gene name synonym)|uniprotkb:Sub1.5(gene name synonym)	psi-mi:gna13_human(display_long)|uniprotkb:GNA13(gene name)|psi-mi:GNA13(display_short)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Bhattacharyya et al. (2003)	pubmed:12681510	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-465471	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_004697.2|refseq:NP_945328.1|refseq:NP_945353.1|reactome:R-HSA-193648|reactome:R-HSA-416482|reactome:R-HSA-8980692|reactome:R-HSA-9013026|reactome:R-HSA-9013106|ensembl:ENSG00000076928(gene)|ensembl:ENST00000354532(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003723"(RNA binding)|go:"GO:0005085"(guanyl-nucleotide exchange factor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007266"(Rho protein signal transduction)|go:"GO:0051056"(regulation of small GTPase mediated signal transduction)|interpro:IPR000219("Dbl homology (DH) domain")|interpro:IPR001849(Pleckstrin-like)|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR015212(Regulator of G protein signalling-like fold)|interpro:IPR035899|interpro:IPR036305|interpro:IPR037887|interpro:IPR041020|mint:Q92888|rcsb pdb:1IAP|rcsb pdb:1SHZ|rcsb pdb:3AB3|rcsb pdb:3ODO|rcsb pdb:3ODW|rcsb pdb:3ODX|rcsb pdb:3P6A	ensembl:ENSG00000120063(gene)|ensembl:ENST00000439174(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003924"(GTPase activity)|go:"GO:0005085"(guanyl-nucleotide exchange factor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0007165"(signal transduction)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007204"(positive regulation of cytosolic calcium ion concentration)|go:"GO:0007266"(Rho protein signal transduction)|go:"GO:0016020"(membrane)|go:"GO:0031526"(brush border membrane)|go:"GO:0031584"(activation of phospholipase D activity)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0031752"(D5 dopamine receptor binding)|go:"GO:0042470"(melanosome)|go:"GO:0046872"(metal ion binding)|go:"GO:0051056"(regulation of small GTPase mediated signal transduction)|go:"GO:0070062"(extracellular exosome)|go:"GO:1904706"(negative regulation of vascular associated smooth muscle cell proliferation)|go:"GO:1904753"(negative regulation of vascular associated smooth muscle cell migration)|interpro:IPR000469(G-protein alpha subunit, group 12)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|reactome:R-HSA-193648|reactome:R-HSA-416482|reactome:R-HSA-428930|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|reactome:R-HSA-9013148|reactome:R-HSA-9013149|reactome:R-HSA-456926|refseq:NP_006563.2|refseq:NP_001269354.1	-	-	-	stimulant:AlF4- is added to block the complex between GAP/G protein, here it enables to detect the P115/Galpha13 interaction. Same experiments were conducted without AlF4-, but with the GTPase-deficient G alpha 13 mutant Gln226Leu.	taxid:9534(chlae-cos_7)|taxid:9534(Cercopithecus aethiops simian cells transformed with SV40)	-	2004/12/06	2014/10/16	rogid:XIZmflM2cr80ZBD3FO21IDMnInE9606	rogid:ZYmlpIogjya3/ejteQS1YC+LqKI9606	intact-crc:A6DB9A58BF166E91|rigid:+A00WVKkm/4W6VEBfgEpo2Tqgxo	false	binding-associated region:22-31|mutation decreasing interaction:27-27,29-29|myc tag:n-n	ha tag:n-n	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)