DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu ANK6, a mitochondrial ankyrin repeat protein, is required for male-female gamete recognition in Arabidopsis thaliana. ANK6, a mitochondrial ankyrin repeat protein, is required for male-female gamete recognition in Arabidopsis thaliana. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Yu F., Shi J., Zhou J., Gu J., Chen Q., Li J., Cheng W., Mao D., Tian L., Buchanan BB., Li L., Chen L., Li D., Luan S. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Yu F., Shi J., Zhou J., Gu J., Chen Q., Li J., Cheng W., Mao D., Tian L., Buchanan BB., Li L., Chen L., Li D., Luan S. DIP original experiment: DIP-254210X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx ANK6, a mitochondrial ankyrin repeat protein, is required for male-female gamete recognition in Arabidopsis thaliana. ANK6, a mitochondrial ankyrin repeat protein, is required for male-female gamete recognition in Arabidopsis thaliana. ANK6, a mitochondrial ankyrin repeat protein, is required for male-female gamete recognition in Arabidopsis thaliana. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Yu F., Shi J., Zhou J., Gu J., Chen Q., Li J., Cheng W., Mao D., Tian L., Buchanan BB., Li L., Chen L., Li D., Luan S. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Yu F., Shi J., Zhou J., Gu J., Chen Q., Li J., Cheng W., Mao D., Tian L., Buchanan BB., Li L., Chen L., Li D., Luan S. DIP original experiment: DIP-254209X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx ANK6, a mitochondrial ankyrin repeat protein, is required for male-female gamete recognition in Arabidopsis thaliana. ANK6, a mitochondrial ankyrin repeat protein, is required for male-female gamete recognition in Arabidopsis thaliana. ANK6, a mitochondrial ankyrin repeat protein, is required for male-female gamete recognition in Arabidopsis thaliana. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Yu F., Shi J., Zhou J., Gu J., Chen Q., Li J., Cheng W., Mao D., Tian L., Buchanan BB., Li L., Chen L., Li D., Luan S. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Yu F., Shi J., Zhou J., Gu J., Chen Q., Li J., Cheng W., Mao D., Tian L., Buchanan BB., Li L., Chen L., Li D., Luan S. DIP original experiment: DIP-254211X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx ANK6, a mitochondrial ankyrin repeat protein, is required for male-female gamete recognition in Arabidopsis thaliana. ANK6, a mitochondrial ankyrin repeat protein, is required for male-female gamete recognition in Arabidopsis thaliana. ANK6, a mitochondrial ankyrin repeat protein, is required for male-female gamete recognition in Arabidopsis thaliana. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Yu F., Shi J., Zhou J., Gu J., Chen Q., Li J., Cheng W., Mao D., Tian L., Buchanan BB., Li L., Chen L., Li D., Luan S. Only protein-protein interactions arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined Yu F., Shi J., Zhou J., Gu J., Chen Q., Li J., Cheng W., Mao D., Tian L., Buchanan BB., Li L., Chen L., Li D., Luan S. DIP original experiment: DIP-254212X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx ANK6, a mitochondrial ankyrin repeat protein, is required for male-female gamete recognition in Arabidopsis thaliana. sige_arath RNA polymerase sigma factor sigE, chloroplastic/mitochondrial SIGE RNA polymerase sigma factor sig5 SIG5 MLE8.4 At5g24120 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGVVSISSSAARSPLGLGNDLLTHRSSLKKPSIVAFKADDSSNSALIIPPREQVLIPAEKHNEKKREVTRRKPCKTPKKPLSLEHNSAPSCSLGVDYNEAAARLESIYKLSPATTTLVEDDVEDGGSKAKVSRRRKRKESGEEKKVVVRNNVKKEKRMSLDKRIALKRNVQEKPVVASVDKKVTKRQQEEEKIERLVRDYSASNDIVSLDWKKMKIPPVLSSTEHAWLFKLMQPMKALLEVKDVLQKSLGREPREAEIAGEINMTAGEVKKKIEIGRAARNKLIKHNLRLVLFVMNKYFHEFTNGPKFQDLCQAGMRGLITAIDRFEPKRKFRLSTYGLFWIRHAIIRSMTTSNFTRVPFGLESVRVEIYKAKTELWFEMGRPPTEDEVVERLKITPERYREVLRAAKPVLSLNSKHSVTQEEFINGITDVDGVGANNSRQPALLRLALDDVLDSLKPKESLVIRQRYGLDGKGDRTLGEIAGNLNISREMVRKHEVKALMKLKHQARVDYLRQYIV DIP protein Q9ZNX9 original sequence version: 75 09FD115934E4FBDD pia2_arath Phytochrome-interacting ankyrin-repeat protein 2 PIA2 ANK6 MAF19.22 At5g61230 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MLQEPSAAFSLRRNSFRRRSPRSNVDDRGWNPLHIKARKGDLKSVKQLLDQGMDVNALAWGPKSKGVSALHLAAEGGHIEVMDLLLERGANIDAKTWGSCGWTPLHAAAKERKREAVKFLVENGAFLADDITDTRFNPPVHYCHGLEWAYEEMKKLNSESSSSSGGDTSSSSDN DIP protein Q9FNP4 original sequence version: 60 22970E70A40A2966 q9fnp4_arath-sige-1 3 5 unspecified role unspecified role prey prey Region 65-517 Region 65-517 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 6 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli physical association physical association q9fnp4_arath-sige-2 2 5 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 6 unspecified role unspecified role bait bait Region 21-174 Region 21-174 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction q9fnp4_arath-sige-3 4 5 unspecified role unspecified role unspecified role unspecified role N-terminal domain of Venus Venus-tag fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress 6 unspecified role unspecified role unspecified role unspecified role C-terminal domain of CFP CFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association q9fnp4_arath-sige-4 1 5 unspecified role unspecified role prey prey Region 66-235 Region 66-235 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Region 148-293 Region 148-293 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 6 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction