IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Physical interaction and mutual transrepression between CCAAT/enhancer-binding protein beta and the p53 tumor suppressor. Physical interaction and mutual transrepression between CCAAT/enhancer-binding protein beta and the p53 tumor suppressor. J. Biol. Chem. (0021-9258) 2006 imex curation Schneider-Merck T., Pohnke Y., Kempf R., Christian M., Brosens JJ., Gellersen B. BioCreative - Critical Assessment of Information Extraction systems in Biology Apoptosis - Interactions involving proteins with a function related to apoptosis Chromatin - Epigenetic interactions resulting in chromatin modulation Cells were incubated in the presence or absence of 8-Br-cAMP for 6 days prior to cell lysis. gellersen@endokrinologikum.com 14-DEC-2006: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Schneider-Merck T., Pohnke Y., Kempf R., Christian M., Brosens JJ., Gellersen B. BioCreative - Critical Assessment of Information Extraction systems in Biology Cells were incubated in the presence or absence of 8-Br-cAMP for 6 days prior to cell lysis. gellersen@endokrinologikum.com Accepted 2006-SEP-7 by JYOTI. 14-DEC-2006: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Physical interaction and mutual transrepression between CCAAT/enhancer-binding protein beta and the p53 tumor suppressor. Physical interaction and mutual transrepression between CCAAT/enhancer-binding protein beta and the p53 tumor suppressor. J. Biol. Chem. (0021-9258) 2006 imex curation Schneider-Merck T., Pohnke Y., Kempf R., Christian M., Brosens JJ., Gellersen B. BioCreative - Critical Assessment of Information Extraction systems in Biology Apoptosis - Interactions involving proteins with a function related to apoptosis Chromatin - Epigenetic interactions resulting in chromatin modulation Cells were incubated in the presence or absence of 8-Br-cAMP for 6 days prior to cell lysis. gellersen@endokrinologikum.com 14-DEC-2006: Contacted by JYOTI. Only protein-protein interactions human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Schneider-Merck T., Pohnke Y., Kempf R., Christian M., Brosens JJ., Gellersen B. BioCreative - Critical Assessment of Information Extraction systems in Biology Cells were incubated in the presence or absence of 8-Br-cAMP for 6 days prior to cell lysis. gellersen@endokrinologikum.com Accepted 2006-SEP-7 by JYOTI. 14-DEC-2006: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Physical interaction and mutual transrepression between CCAAT/enhancer-binding protein beta and the p53 tumor suppressor. Physical interaction and mutual transrepression between CCAAT/enhancer-binding protein beta and the p53 tumor suppressor. J. Biol. Chem. (0021-9258) 2006 imex curation Schneider-Merck T., Pohnke Y., Kempf R., Christian M., Brosens JJ., Gellersen B. BioCreative - Critical Assessment of Information Extraction systems in Biology Apoptosis - Interactions involving proteins with a function related to apoptosis Chromatin - Epigenetic interactions resulting in chromatin modulation Cells were incubated in the presence or absence of 8-Br-cAMP for 6 days prior to cell lysis. gellersen@endokrinologikum.com 14-DEC-2006: Contacted by JYOTI. Only protein-protein interactions human-endomet_stroma_cells Human endometrial stromal cells endometrial_stroma endometrial stroma Purified human ESCs were prepared from anonymized biopsy samples obtained from premenopausal women at the time of hysterectomy for benign gynecological disorders. fluorescence imaging fluorescence microscopy fluorescence imaging polyclonal immunost polyclonal antibody immunostaining polyclonal immunost Schneider-Merck T., Pohnke Y., Kempf R., Christian M., Brosens JJ., Gellersen B. BioCreative - Critical Assessment of Information Extraction systems in Biology Cells were incubated in the presence or absence of 8-Br-cAMP for 6 days prior to cell lysis. gellersen@endokrinologikum.com Accepted 2006-SEP-7 by JYOTI. 14-DEC-2006: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Physical interaction and mutual transrepression between CCAAT/enhancer-binding protein beta and the p53 tumor suppressor. Physical interaction and mutual transrepression between CCAAT/enhancer-binding protein beta and the p53 tumor suppressor. J. Biol. Chem. (0021-9258) 2006 imex curation Schneider-Merck T., Pohnke Y., Kempf R., Christian M., Brosens JJ., Gellersen B. BioCreative - Critical Assessment of Information Extraction systems in Biology Apoptosis - Interactions involving proteins with a function related to apoptosis Chromatin - Epigenetic interactions resulting in chromatin modulation Cells were incubated in the presence or absence of 8-Br-cAMP for 6 days prior to cell lysis. gellersen@endokrinologikum.com 14-DEC-2006: Contacted by JYOTI. Only protein-protein interactions human-endomet_stroma_cells Human endometrial stromal cells endometrial_stroma endometrial stroma Purified human ESCs were prepared from anonymized biopsy samples obtained from premenopausal women at the time of hysterectomy for benign gynecological disorders. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Schneider-Merck T., Pohnke Y., Kempf R., Christian M., Brosens JJ., Gellersen B. BioCreative - Critical Assessment of Information Extraction systems in Biology Cells were incubated in the presence or absence of 8-Br-cAMP for 6 days prior to cell lysis. gellersen@endokrinologikum.com Accepted 2006-SEP-7 by JYOTI. 14-DEC-2006: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation cebpb_human CCAAT/enhancer-binding protein beta CEBPB TCF5 PP9092 Liver activator protein Nuclear factor NF-IL6 Transcription factor 5 Liver-enriched inhibitory protein protein protein human Homo sapiens Human MQRLVAWDPACLPLPPPPPAFKSMEVANFYYEADCLAAAYGGKAAPAAPPAARPGPRPPAGELGSIGDHERAIDFSPYLEPLGAPQAPAPATATDTFEAAPPAPAPAPASSGQHHDFLSDLFSDDYGGKNCKKPAEYGYVSLGRLGAAKGALHPGCFAPLHPPPPPPPPPAELKAEPGFEPADCKRKEEAGAPGGGAGMAAGFPYALRAYLGYQAVPSGSSGSLSTSSSSSPPGTPSPADAKAPPTACYAGAAPAPSQVKSKAKKTVDKHSDEYKIRRERNNIAVRKSRDKAKMRNLETQHKVLELTAENERLQKKVEQLSRELSTLRNLFKQLPEPLLASSGHC DIP protein P17676 original sequence version: 102 9B725BD6CCAA771D p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 tp53-lip 1 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association Wild type and mutant 35S-labeled p53 proteins were subjected to pull-down assays with GST-LAP, GST-LIP, or GST alone immobilized on glutathione-Sepharose. The input of labeled protein is shown in the first of four lanes for each of the p53 variants. Figure 4 tp53-cebpb 2 5 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association Detection of immunoprecipitated samples with C/EBP{beta} antibody revealed the presence of LIP-FLAG in those cases where WT p53 or p53.M175 had been co-transfected Figure 5A The LIP protein arises from CCAAT/enhancer-binding protein beta mRNA which is initiated at the downstream codon Met199 giving rise to the 16-kDa repressor LIP protein. tp53-lap-1 4 5 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey physical association physical association p53 and C/EBP interact in vivo. Sufficient to bind region of cebpb_human is [1-345] or [24-345] due to the ambiguity of LAP arising from Met-1 or Met24 being used as initiatior methionine. Figure 5B The LAP protein arises from CCAAT/enhancer-binding protein beta mRNA. Partial colocalisation of the proteins to punctate nuclear structures was reported. tp53-lap-2 3 6 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain colocalization colocalization Figure 6 The LAP protein arises from CCAAT/enhancer-binding protein beta mRNA. There is an ambiguity concerning the exact sequence of the LAP protein which is documented as arising from Met1 or Met24 of the CCAAT/enhancer-binding protein beta. Partial colocalisation of the proteins to the punctate nucler structures was reported.