CHEK1
label: CHK1
label: UniProt:O14757 CHEK1

UniProt:P30291 WEE1
label: WEE1

ATM
label: UniProt:Q13315 ATM

Phosphorylation of Cdc25A at Ser-123 by Chk1
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: Restriction
  on property: enabled by
  some values from: CHEK1
type: Restriction
  on property: enabled by
  some values from: CHEK1
type: GO_0016301
BFO_0000050: Activation of ATR in response to replication stress

Recruitment and activation of Chk1
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: GO_0004674
type: GO_0006260
BFO_0000050: G2/M DNA damage checkpoint
BFO_0000050: Activation of ATR in response to replication stress
directly activates: Phosphorylation of Cdc25A at Ser-123 by Chk1
directly activates: Phosphorylation of Cdc25C at Ser 216 by Chk1
directly activates: Catalysis814
directly activates: Catalysis822

Phosphorylation and activation of CHK2 by ATM
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: Restriction
  on property: enabled by
  some values from: ATM
type: Restriction
  on property: enabled by
  some values from: ATM
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: GO_0016301
BFO_0000050: G2/M DNA damage checkpoint
directly activates: BiochemicalReaction1842
directly activates: Catalysis810

Phosphorylation of Cdc25C at Ser216
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: GO_0016301
BFO_0000050: Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex
directly activates: Association of phospho-Cdc25C(Ser 216) with 14-3-3 proteins

Association of phospho-Cdc25C(Ser 216) with 14-3-3 proteins
type: GO_0003674
BFO_0000050: Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex
directly activates: Retention of phospho-Cdc25C:14-3-3 complexes within the cytoplasm

Retention of phospho-Cdc25C:14-3-3 complexes within the cytoplasm
type: GO_0003674
BFO_0000050: Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex

Phosphorylation of Wee1 kinase by Chk1
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: Restriction
  on property: enabled by
  some values from: CHEK1
type: Restriction
  on property: enabled by
  some values from: CHEK1
type: Restriction
  on property: enabled by
  some values from: CHEK1
type: Restriction
  on property: enabled by
  some values from: CHEK1
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: GO_0016301
BFO_0000050: Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex
directly activates: Wee1-mediated phosphorylation of Cyclin B1:phospho-Cdc2 complexes
directly activates: Catalysis819

Wee1-mediated phosphorylation of Cyclin B1:phospho-Cdc2 complexes
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: Restriction
  on property: enabled by
  some values from: UniProt:P30291 WEE1
type: Restriction
  on property: enabled by
  some values from: UniProt:P30291 WEE1
type: Restriction
  on property: enabled by
  some values from: UniProt:P30291 WEE1
type: Restriction
  on property: enabled by
  some values from: UniProt:P30291 WEE1
type: Restriction
  on property: enabled by
  some values from: UniProt:P30291 WEE1
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: Restriction
  on property: enabled by
  some values from: UniProt:P30291 WEE1
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: GO_0016301
BFO_0000050: Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex
BFO_0000050: G2/M DNA replication checkpoint
directly activates: BiochemicalReaction1942
directly activates: Control139

Myt-1 mediated phosphorylation of Cyclin B:Cdc2 complexes
type: GO_0003674
BFO_0000050: G2/M DNA replication checkpoint
directly activates: BiochemicalReaction1940
directly activates: BiochemicalReaction1950
directly activates: Catalysis864

Stalling of DNA replication fork and RPA binding
type: GO_0006260
BFO_0000050: Activation of ATR in response to replication stress
directly activates: Binding of ATR-ATRIP to the RPA-ssDNA complex
directly activates: Recruitment of Rad17-RFC complex to DNA

Binding of ATR-ATRIP to the RPA-ssDNA complex
type: GO_0006260
BFO_0000050: Activation of ATR in response to replication stress
directly activates: Activation of claspin
directly activates: Catalysis821

Recruitment of Rad17-RFC complex to DNA
type: GO_0006260
BFO_0000050: Activation of ATR in response to replication stress
directly activates: Recruitment of the 9-1-1 complex to DNA
directly activates: Catalysis820

Recruitment of the 9-1-1 complex to DNA
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: GO_0003689
type: GO_0006260
BFO_0000050: Activation of ATR in response to replication stress
directly activates: Recruitment and activation of Chk1
directly activates: Catalysis815
label: Recruitment of the Rad9-Hus1-Rad1 complex to DNA

Loading of claspin onto DNA during replication origin firing
type: GO_0006260
BFO_0000050: Activation of ATR in response to replication stress
directly activates: Activation of claspin
directly activates: Catalysis821

Activation of claspin
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: GO_0004674
type: GO_0006260
BFO_0000050: Activation of ATR in response to replication stress
directly activates: Recruitment and activation of Chk1
directly activates: Catalysis815

Phosphorylation of Cdc25C at Ser 216 by Chk1
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005654
type: Restriction
  on property: enabled by
  some values from: CHEK1
type: Restriction
  on property: enabled by
  some values from: CHEK1
type: GO_0004674
BFO_0000050: Activation of ATR in response to replication stress

G2/M Checkpoints
type: GO_0008150

G2/M DNA damage checkpoint
type: GO_0008150
BFO_0000050: G2/M Checkpoints

Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex
type: GO_0008150
BFO_0000050: G2/M DNA damage checkpoint

G2/M DNA replication checkpoint
type: GO_0008150
BFO_0000050: G2/M Checkpoints

Activation of ATR in response to replication stress
type: GO_0008150
BFO_0000050: G2/M Checkpoints