CHEK1
CHK1
UniProt:O14757 CHEK1
CDS1
CHEK2
CHK2
RAD53
UniProt:O96017-12 CHEK2
UniProt:P30291 WEE1
WEE1
ATM
UniProt:Q13315 ATM
COP1
RFWD2
RNF200
UniProt:Q8NHY2 RFWD2
Phosphorylation of p53 at ser-15 by ATM kinase
Phosphorylation of MDM2 at serine-395 by ATM kinase
Phosphorylation of COP1 at Ser-387 by ATM
Dissociation of the COP1-p53 complex
Translocation of COP1 from the nucleus to the cytoplasm
Autoubiquitination of phospho-COP1(Ser-387 )
Proteasome mediated degradation of COP1
Inactivation of Cyclin E:Cdk2 complexes by p27/p21
Phosphorylation of Cdc25A at Ser-123 in response to DNA damage
Phosphorylation of Cdc25A at Ser-123 by Chk2
Ubiquitination of phosphorylated Cdc25A
Proteolytic degradation of ubiquitinated-Cdc25A
Phosphorylation and activation of Chk1 by ATM
Phosphorylation of Cdc25A at Ser-123 by Chk1
Recruitment and activation of Chk1
Phosphorylation and activation of CHK2 by ATM
Phosphorylation of Cdc25C at Ser216
Association of phospho-Cdc25C(Ser 216) with 14-3-3 proteins
Retention of phospho-Cdc25C:14-3-3 complexes within the cytoplasm
Phosphorylation of Wee1 kinase by Chk1
Wee1-mediated phosphorylation of Cyclin B1:phospho-Cdc2 complexes
Myt-1 mediated phosphorylation of Cyclin B:Cdc2 complexes
Stalling of DNA replication fork and RPA binding
Binding of ATR-ATRIP to the RPA-ssDNA complex
Recruitment of Rad17-RFC complex to DNA
Recruitment of the 9-1-1 complex to DNA
Recruitment of the Rad9-Hus1-Rad1 complex to DNA
Loading of claspin onto DNA during replication origin firing
Activation of claspin
Phosphorylation of Cdc25C at Ser 216 by Chk1
Mad1 binds kinetochore
MAD2 associates with the Mad1 kinetochore complex
MAD2 converted to an inhibitory state via interaction with Mad1
Release of activated MAD2 from kinetochores
Inactivation of APC/C via CDC20 sequestration
Formation of the MCC complex
Binding of the MCC complex to the APC/C complex
Cell Cycle Checkpoints
G1/S DNA Damage Checkpoints
p53-Dependent G1/S DNA damage checkpoint
p53-Dependent G1 DNA Damage Response
Stabilization of p53
Autodegradation of the E3 ubiquitin ligase COP1
Transcriptional activation of p53 responsive genes
Transcriptional activation of cell cycle inhibitor p21
p53-Independent G1/S DNA damage checkpoint
p53-Independent DNA Damage Response
Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
G2/M Checkpoints
G2/M DNA damage checkpoint
Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex
G2/M DNA replication checkpoint
Activation of ATR in response to replication stress
Mitotic Spindle Checkpoint
Amplification of signal from the kinetochores
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components
Inactivation of APC/C via direct inhibition of the APC/C complex
Transcriptional activation of p21 by p53 after DNA damage