# HISTORY
24 Mar 2016: Updated by: TOUCHUP-v1.15
14 Mar 2016: Updated by: TOUCHUP-v1.14

# molecular_function
20150626: root_PTN000206333 has function asparagine synthase (glutamine-hydrolyzing) activity (GO:0004066) 
20150626: root_PTN000206333 has function protein homodimerization activity (GO:0042803) 
20150626: Embryophyta_PTN000925225 has LOST/MODIFIED function asparagine synthase (glutamine-hydrolyzing) activity (GO:0004066) 
20150626: Embryophyta_PTN000925225 has LOST/MODIFIED function protein homodimerization activity (GO:0042803) 

# cellular_component
20150626: node_PTN000206336 is found in cytosol (GO:0005829) 
20150626: node_PTN000925259 is found in cytosol (GO:0005829) 

# biological_process
20150626: root_PTN000206333 participates in asparagine biosynthetic process (GO:0006529) 
20150626: root_PTN000206333 participates in glutamine metabolic process (GO:0006541) 
20150626: Magnoliophyta_PTN001717383 participates in response to absence of light (GO:0009646) 
20150626: Embryophyta_PTN000925225 does NOT participate in asparagine biosynthetic process (GO:0006529) 

# WARNINGS - THE FOLLOWING HAVE BEEN REMOVED FOR THE REASONS NOTED

# NOTES
- EC 6.3.5.4
- the coli homolog asnB has a structure - pmid:10587437
C1-D194: N-term domain (Q hydrolysis)
* W195-G516: C-term domain - binding of both Mg2+ATP and aspartate
* Ntn type glutamine amidotransferases
* C1 is part of the active site
* R49, N74, E76, D98 bind to glutamine
* the bacterial enzyme is a dimer
* it is cytosolic
- pmid:6105958 - both yeast proteins, asn1/2, are localized to the cytosol -did not see any diff between them(localization, activity, regulation)
- pmid:9881155 - AT asn genes
* there are 3 proteins in AT, asn1-3
* "Like ASN1, the ASN2 and ASN3 proteins each contain the conserved residues of a PurF-type glutamine-binding triad?
* asn1 is regulated inversely to asn2 (light vs. dark, etc)
- PTN000925225 doesn?t have the right sequence conservation to do the glutamine hydrolysis part

# REFERENCE
Annotation inferences using phylogenetic trees
The goal of the GO Reference Genome Project, described in PMID 19578431, is to provide accurate, complete and consistent GO annotations for all genes in twelve model organism genomes. To this end, GO curators are annotating evolutionary trees from the PANTHER database with GO terms describing molecular function, biological process and cellular component. GO terms based on experimental data from the scientific literature are used to annotate ancestral genes in the phylogenetic tree by sequence similarity (ISS), and unannotated descendants of these ancestral genes are inferred to have inherited these same GO annotations by descent. The annotations are done using a tool called PAINT (Phylogenetic Annotation and INference Tool).