---------------------------------------------------------------------------- UniProt - Swiss-Prot Protein Knowledgebase SIB Swiss Institute of Bioinformatics; Geneva, Switzerland European Bioinformatics Institute (EBI); Hinxton, United Kingdom Protein Information Resource (PIR); Washington DC, USA ---------------------------------------------------------------------------- Description: Restriction enzymes and methylases: classification and list of entries Name: restric.txt Release: 2022_03 of 03-Aug-2022 ---------------------------------------------------------------------------- Further informations about restriction enzymes and their associated methylases is available from: http://rebase.neb.com/ ============================== Type II enzymes and methylases ============================== _________ _________________________ ____________________ ____ ____________________ Name Recognition site(s) Restriction Type Methylase(s) of enzyme enzyme of Entry name AC met. Entry name AC _________ _________________________ ____________________ ____ ____________________ AbrI C^TCGAG T2A1_AZOBR (P26919) 6mA MTA1_AZOBR (P58284) AccI GT^MKAC T2A1_ACICA (P24546) 6mA MTA1_ACICA (P25201) AgeI A^CCGGT T2A1_THAGE (Q9KHV6) 5mC MTA1_THAGE (P94147) AluI AG^CT N.A. 5mC MTA1_CELCE (P31974) ApaLI G^TGCAC T2A1_ACEPA (O52703) 5mC MTA1_ACEPA (O52702) AquI C^YCGRG N.A. 5mC MTAA_SYNP2 (P34882) MTAB_SYNP2 (P34883) AvaI C^YCGRG T2A1_NOSS1 (P0A457) 4mC MTA1_NOSS1 (P0A461) AvaI C^YCGRG T2A1_ANAVA (P0A458) 4mC MTA1_ANAVA (P0A462) BamHI G^GATCC T2BA_BACAM (P23940) 4mC MTB1_BACAM (P23941) BamHII GGATCC N.A. 4mC MTB2_BACAM (P18051) BanI G^GYRCC T2BA_ANEAE (P19887) 5mC MTBA_ANEAE (P19888) BanIII AT^CGAT N.A. 6mA MTB3_ANEAE (P22772) BbvI GCAGC (13); GCTGC (-12) N.A. 5mC MTB1_BREBE (P34905) BepI CG^CG N.A. 5mC MTB1_BREEP (P10283) BglI GCCNNNN^NGGC T2B1_BACIU (O68557) 4mC MTB1_BACIU (O68556) BglII A^GATCT T2B2_BACIU (Q45488) 4mC MTB2_BACIU (Q45489) BseCI AT^CGAT N.A. 5mC MTC1_GEOSE (P43423) BslI CCNNNNN^NNGG T2BLA_BACSQ (Q9LAI1) 4mC MTB1_BACSQ (Q9LAI2) T2BLB_BACSQ (Q9LAI0) BsoBI C^YCGRG T2B1_GEOSE (P70985) 4mC MTB1_GEOSE (P70986) Bsp6I GC^NGC T2B6_BACSF (P43419) 5mC MTB6_BACSF (P43420) BspRI GG^CC N.A. 5mC MTB1_LYSSH (P13906) BstVI C^TCGAG T2V1_GEOSE (P43421) 6mA MTV1_GEOSE (P43422) BsuBI CTGCAG T2BB_BACIU (P33562) 6mA MTBB_BACIU (P33563) BsuFI C^CGG T2BF_BACIU (P25217) 5mC MTBF_BACIU (P17044) BsuRI GG^CC T2BR_BACIU (P06529) 5mC MTBR_BACIU (P06530) CeqI GAT^ATC T2C1_RHOHA (P42827) 6mA MTC1_RHOHA (P42828) Cfr9I C^CCGGG T2C9_CITFR (Q60132) 4mC MTC9_CITFR (P14243) Cfr10I R^CCGGY T2CX_CITFR (P56200) 5mC N.A. CfrBI C^CWWGG T2C1_CITFR (Q04852) 4mC MTC1_CITFR (Q04845) CviAII C^ATG T2C2_PBCV1 (P31117) 6mA MTC2_PBCV1 (P31118) CviBI G^ANTC N.A. 6mA MTC1_PBCVC (Q01511) CviJI RG^CY T2C1_PBCVI (P52283) 5mC MTC1_PBCVI (P36216) CviRI TG^CA N.A. 6mA MTR1_PBCVX (P52284) DdeI C^TNAG T2D1_DESNO (P05301) 5mC MTD1_DESNO (P05302) DpnI GA^TC T2D1_STRPN (P0A459) ? N.A. DpnII ^GATC T2D2_STREE (P09357) 6mA MTD21_STREE (P04043) MTD22_STREE (P09358) DsaV ^CCNGG N.A. 5mC MTD5_DACSA (P50185) EcaI G^GTNACC N.A. 6mA MTEC_ENTCL (P14827) Eco47I G^GWCC T2E7_ECOLX (P50194) ? N.A. Eco47II G^GNCC T2E8_ECOLX (P50195) 5mC MTE8_ECOLX (P50196) Eco57I CTGAAG (22); CTTCAG (-14) T257_ECOLX (P25239) 6mA MT57_ECOLX (P25240) EcoRI G^AATTC T2E1_ECOLX (P00642) 6mA MTE1_ECOLX (P00472) EcoRII ^CCWGG T2E2_ECOLX (P14633) 5mC MTE2_ECOLX (P05101) EcoRV GAT^ATC T2E5_ECOLX (P04390) 6mA MTE5_ECOLX (P04393) FnuDI GG^CC N.A. 5mC MTF1_FUSNU (P34906) FokI GGATG (14); CATCC (-13) T2F1_PLAOK (P14870) 6mA MTF1_PLAOK (P14871) HaeII RGCGC^Y T2H2_HAEAE (O30869) 5mC MTH2_HAEAE (O30868) HaeIII GG^CC T2H3_HAEAE (O68584) 5mC MTH3_HAEAE (P20589) HgaI GACGC (10); GCGTC (-10) T2G1_AVIPA (P43418) 5mC MTG1_AVIPA (P25282) MTG2_AVIPA (P25283) HgiBI G^GWCC T2B1_HERAU (P25257) 5mC MTB1_HERAU (P25262) HgiCI G^GYRCC T2C1_HERAU (P25258) 5mC MTC1_HERAU (P25263) HgiCII G^GWCC T2C2_HERAU (P25259) 5mC MTC2_HERAU (P25264) HgiDI GR^CGYC T2D1_HERAU (P24599) 5mC MTD1_HERAU (P24600) HgiDII G^TCGAC N.A. 5mC MTD2_HERAU (P25265) HgiEI G^GWCC T2E1_HERAU (P25260) 5mC MTE1_HERAU (P25266) HgiGI GR^CGYC T2G1_HERAU (P25261) 5mC MTG1_HERAU (P25267) HhaI GCG^C N.A. 5mC MTH1_HAEPH (P05102) HhaII G^ANTC T2H2_HAEPH (P00643) 6mA MTH2_HAEPH (P00473) HincII GTY^RAC T2C2_HAEIF (P17743) 6mA MTHC_HAEIF (P17744) HindII GTY^RAC T2D2_HAEIN (P44413) 6mA MTHD_HAEIN (P44414) HindIII A^AGCTT T2D3_HAEIN (P43870) 6mA MTH3_HAEIN (P43871) HindV GRCGYC T2D5_HAEIN (P44999) 5mC MTH5_HAEIN (P45000) HinfI G^ANTC T2F1_HAEIF (P20588) 6mA MTH1_HAEIF (P20590) HpaI GTT^AAC T2H1_HAEPA (P29537) 6mA MTH1_HAEPA (P29538) HpaII C^CGG T2H2_HAEPA (P36433) 5mC MTH2_HAEPA (P15446) HphI GGTGA (13); TCACC (-7) T2H1_HAEPH (P50191) 5mC MTHA_HAEPH (P50192) 6mA MTHB_HAEPH (P50193) KpnI GGTAC^C T2K1_KLEPN (P25237) 6mA MTK1_KLEPN (P25238) LlaDCHI ^GATC T2L2_LACLC (P50177) 6mA MTL21_LACLC (P50179) MTL22_LACLC (P50178) MamI GATNN^NNATC T2M1_MICAM (P50189) 6mA MTM1_MICAM (P50190) MboI ^GATC T2M1_MORBO (P34719) 6mA MT1A_MORBO (P34720) MT1B_MORBO (P34721) MboII GAAGA (13); TCTTC (-7) T2M2_MORBO (P23191) 6mA MTM2_MORBO (P23192) MjaI C^TAG T2M1_METJA (Q58391) 4mC MTM1_METJA (Q58392) MjaII CCNG T2M2_METJA (Q58844) 4mC MTM2_METJA (Q58843) MjaIII ^GATC T2M3_METJA (Q58017) 6mA MTM3_METJA (Q58015) MjaIV GTNNAC T2M4_METJA (Q58723) ? MTM4_METJA (Q58724) MjaV GTAC T2M5_METJA (Q58895) 4mC MTM5_METJA (Q58893) MjaVI CCGG T2M6_METJA (P81326) 4mC MTM6_METJA (Q58606) MspI C^CGG T2M1_MORSP (P11405) 5mC MTM1_MORSP (P11408) MthTI GGCC T2MT_METTF (P29565) 5mC MTHT_METTF (P29567) MthZI C^TAG T2MZ_METTF (P29566) 4mC MTHZ_METTF (P29568) MunI C^AATTG T2MU_MYCSP (P43642) 6mA MTMU_MYCSP (P43641) MvaI CC^WGG N.A. 4mC MTMV_KOCVA (P14244) MwoI GCNNNNN^NNGC T2MW_METWO (O59646) 4mC MTMW_METWO (O59647) NaeI GCC^GGC T2N1_NOCAE (P50187) 5mC MTN1_NOCAE (P50188) NgoBI RGCGC^Y T2B1_NEIGO (Q50973) 5mC MTB1_NEIGO (Q59603) NgoBV GGN^NCC T2B5_NEIGO (Q50975) 5mC MTB5_NEIGO (Q59605) NgoFVII GCSG^C T2F7_NEIGO (Q50976) 5mC MTF7_NEIGO (Q59606) NgoMIV G^CCGGC T2M4_NEIGO (P31032) 5mC MTM4_NEIGO (P31033) NgoPII GG^CC T2P2_NEIGO (P24617) 5mC MTP2_NEIGO (P08455) NlaIII CATG^ T2N3_NEILA (Q51083) 6mA MTN3_NEILA (P24582) NlaIV GGN^NCC T2N4_NEILA (P50183) 5mC MTN4_NEILA (P50182) NmeDI ? T2D1_NEIMC (Q9RLM3) 5mC MTD1_NEIMC (Q9RLM4) NspV TT^CGAA T2N5_NOSS7 (P35677) ? MTN5_NOSS7 (P35678) PaeR7I C^TCGAG T2P7_PSEAI (P05104) 6mA MTP7_PSEAI (P05103) PspPI G^GNCC N.A. 5mC MTP1_PSYTA (O33481) PstI CTGCA^G T2PS_PROST (P00640) 6mA MTPS_PROST (P00474) PvuI CGAT^CG T2P1_PROHU (P31031) ? N.A. PvuII CAG^CTG T2P2_PROHU (P23657) 4mC MTP2_PROHU (P11409) RsrI G^AATTC T2R1_RHOSH (P21763) 6mA MTR1_RHOSH (P14751) SacI GAGCT^C T2S1_STRAH (O31074) 5mC MTS1_STRAH (O31073) SalI G^TCGAC T2S1_STRAL (Q53608) 6mA MTS1_STRAL (Q53609) Sau3AI ^GATC T2S3_STAAU (P16667) 5mC MTS3_STAAU (P16668) Sau96I G^GNCC T2S9_STAAU (P23736) 5mC MTS9_STAAU (P23737) ScaI AGT^ACT T2S1_STRCS (O52691) 4mC MTS1_STRCS (O52692) ScrFI CC^NGG T2S1_LACLC (O34104) 5mC MTSA_LACLC (P34877) MTSB_LACLC (P34878) SfiI GGCCNNNN^NGGCC T2S1_STRFI (O52512) 4mC MTS1_STRFI (O52513) SinI G^GWCC T2S1_SALIN (P09796) 5mC MTS1_SALIN (P09795) SmaI CCC^GGG T2SM_SERMA (P14229) 4mC MTSM_SERMA (P14230) SsoII ^CCNGG T2S2_SHISO (P34880) 5mC MTS2_SHISO (P34879) StsI GGATG (15); CATCC (-14) T2S1_STRSA (P29346) 6mA MTS1_STRSA (P29347) TaqI T^CGA T2TA_THEAQ (P14386) 6mA MTTA_THEAQ (P14385) TthHB8I T^CGA T2T8_THET8 (P29748) 6mA MTT8_THET8 (P29749) VspI AT^TAAT N.A. 6mA MTV1_VIBS3 (Q03055) XamI G^TCGAC T2X1_XANCR (P96189) 6mA MTX1_XANCR (P96188) XcyI C^CCGGG T2X1_XANCC (P30773) 4mC MTX1_XANCC (P30774) XhoI C^TCGAG T2X1_XANVA (Q9KVZ7) 6mA MTX1_XANVA (Q9KVZ8) XorII CGATC^G N.A. 5mC MTX2_XANOR (P52311) M.BabI GANTC 6mA MTB1_BRUAB (P0C116) MTB1_BRUA2 (Q2YMK2) M.BsuSpb GGCC; GCNGC 5mC MTBB_BPSPB (P09389) M.BsuSprI GGCC; CCGG 5mC MTBS_BPSPR (P00476) M.CcrmI GANTC 6mA MTC1_CAUVN (B8GZ33) M.CviBIII TCGA 6mA MTC3_PBCVC (P10835) M.EcoDcm CCWGG 5mC DCM_ECOLI (P0AED9) M.EcoDcm CCWGG 5mC DCM_ECO57 (P0AEE0) M.EcoDam GATC 6mA DMA_ECOLI (P0AEE8) M.EcoDam GATC 6mA DMA_ECO57 (P0AEE9) M.EcoT2Dam GATC 6mA DMA_BPT2 (P12427) M.EcoT4Dam GATC 6mA DMA_BPT4 (P04392) M.Eco67Dam GATC 6mA DMA7_ECOLX (P21311) M.HindIV GATC 6mA DMA_HAEIN (P44431) M.LlaI ? 6mA MTL1_LACLL (P35516) M.NlaX CCNGG 5mC MTNX_NEILA (P24581) M.Phi3TI GGCC; GCNGC 5mC MTBP_BACSU (P68585) M.Phi3TI GGCC; GCNGC 5mC MTBP_BPPHT (P68586) M.Rho11sI GGCC; GAGCTC 5mC MTBR_BPRH1 (P09915) M.SmaII GATC 6mA DMA_SERMA (P45454) M.SmeI GANTC 6mA MTS1_RHIME (O30569) M.StyDam GATC 6mA DMA_SALTY (P0DMP3) M.SPbetaI GGCC 5mC MTBB_BPSPB (P09389) M.SPRI GGCC 5mC MTBS_BPSPR (P00476) M.SssI CG 5mC MTSI_SPISQ (P15840) M.SpomI CCWGG 5mC PMT1M_SCHPO (P40999) M.TpaI GATC 6mA DMA_TREPA (O33844) M.VchADam GATC 6mA DMA_VIBCH (P0C6Q8) ============== Type I enzymes ============== _________ ___________ ____________________ ____________________ ____________________ Name of Recognition S protein R protein M protein enzyme sequence Entry name AC Entry name AC Entry name AC _________ ___________ ____________________ ____________________ ____________________ EcoAI GAGN(7)GTCA T1SA_ECOLX (P19704) T1RA_ECOLX (Q07736) N.A. EcoBI TGAN(8)TGCT T1SB_ECOLX (P06990) N.A. N.A. EcoDI TTAN(7)GTCY T1SD_ECOLX (P06991) N.A. N.A. EcoEI GAGN(7)ATGC T1SE_ECOLX (P19705) T1RE_ECOLX (Q47281) T1ME_ECOLX (Q47282) EcoKI AACN(6)GTGC T1SK_ECOLI (P05719) T1RK_ECOLI (P08956) T1MK_ECOLI (P08957) EcoprrI CCAN(7)ATGC T1SP_ECOLX (P17222) T1RP_ECOLX (P17224) T1MP_ECOLX (Q47163) EcoR124II GAAN(6)RTCG T1S1_ECOLX (P10485) T1R1_ECOLX (P10486) T1M1_ECOLX (P10484) StySPI AACN(6)GTRC T1S_SALPO (P07990) N.A. T1M_SALPO (P07989) StySJI GAGN(6)GTRC T1S_SALTY (P06187) N.A. T1M_SALTY (P40813) MjaX ? T1SH_METJA (Q60296) T1RH_METJA (Q60295) T1MH_METJA (Q60297) _________ ___________ ____________________ ____________________ ____________________ ================ Type III enzymes ================ __________ _________________________ ____________________ ____________________ Name of Recognition sequence Mod protein Res protein enzyme Entry name AC Entry name AC __________ _________________________ ____________________ ____________________ EcoPI AGACC (?); GGTCT (?) T3MO_BPP1 (P08763) T3RE_BPP1 (P08764) EcoP15I CAGCAG (31); CTGCTG (-27) T3MO_ECOLX (P12364) N.A. StyLTI CAGAG (?); CTCTG (?) T3MO_SALTY (P40814) T3RE_SALTY (P40815) Bce10987IP ?; ? N.A. T3RE_BACC1 (P25241) HindVIP ?; ? T3MH_HAEIN (P71366) T3RH_HAEIN (P44105) __________ _________________________ ____________________ ____________________ =============== Type IV enzymes =============== _________ ____________________________________ ____________________ ____________________ Name of Recognition sequence Subunit A Subunit B enzyme entry name AC entry name AC _________ ____________________________________ ____________________ ____________________ BcgI GCANNNNNNTCG (24) CGANNNNNNTGC (-10) T4BA_BACCO (Q07605) T4BB_BACCO (Q07606) CGANNNNNNTGC (24) GCANNNNNNTCG (-10) _________ ____________________________________ ____________________ ____________________ ----------------------------------------------------------------------- Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License -----------------------------------------------------------------------