#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B intact:EBI-26972469 wwpdb:7ctt - intact:EBI-25564960|wwpdb:6xqb|emdb:EMD-22288|wwpdb:6M71|wwpdb:7btf|emdb:EMD-30178|emdb:EMD-30127|intenz:2.7.7.48|wwpdb:7bv2|emdb:EMD-30210|wwpdb:7bv1|emdb:EMD-30209|wwpdb:6x2g|wwpdb:7bw4|emdb:EMD-30226|wwpdb:7l1f|emdb:EMD-23109|wwpdb:6yyt|emdb:EMD-11007|wwpdb:7c2k|emdb:EMD-30275|wwpdb:7bzf|emdb:EMD-30252|reactome:R-COV-9691364|wwpdb:7aap|emdb:EMD-11692|wwpdb:7d4f|emdb:EMD-30572|emdb:EMD-23009|wwpdb:7krp|wwpdb:7b3b|emdb:EMD-11993|wwpdb:7b3c|emdb:EMD-11994|wwpdb:7b3d|emdb:EMD-11995|rhea:"RHEA:21248"|wwpdb:7ozv|emdb:EMD-13138|wwpdb:7ozu|emdb:EMD-13135|wwpdb:7oyg|emdb:EMD-13116 psi-mi:btc5_p_strand_40_mer(display_short)|psi-mi:EBI-26972469(display_long) psi-mi:nsp7-nsp8-nsp12_sars2(display_short)|psi-mi:7ctt(display_long)|intact:SARS-CoV-2 polymerase complex(complex recommended name)|intact:"NSP7:2xNSP8:NSP12"(complex systematic name)|intact:replication-transcription complex(complex synonym)|intact:replication and transcription complex(complex synonym)|intact:RTC(complex synonym)|intact:central RTC(complex synonym)|intact:C-RTC(complex synonym)|intact:holo-RdRp complex(complex synonym)|intact:holo-RNA-dependent RNA polymerase complex(complex synonym) psi-mi:"MI:0410"(3D electron microscopy) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972376|emdb:EMD-23007|imex:IM-28942-1|wwpdb:7krn - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:1302"(stable complex) - intact:EBI-25506373(exp-evidence)|pubmed:32277040(see-also)|go:"GO:0003968"(RNA-directed 5'-3' RNA polymerase activity)|go:"GO:0003725"(double-stranded RNA binding)|go:"GO:0039694"(viral RNA genome replication)|efo:"EFO:0000694"(see-also)|pubmed:16228002(see-also)|go:"GO:0031381"(viral RNA-directed RNA polymerase complex)|pubmed:19875418(see-also)|pubmed:31138817(see-also)|pubmed:31987001(see-also)|pubmed:18255185(see-also)|complex portal:CPX-5742(complex-primary)|pubmed:32015508(see-also)|evidence ontology:"ECO:0000353" - - curated-complex:"RNA-directed 5'-3' RNA polymerase of the SARS-CoV-2 coronavirus which consists of the main polymerase protein NSP12 and a stoichiometric variant of the primase complex (CPX-5690). Extends partially double-stranded RNA templates and is probably also required for transcription initiation, though the mechanism for this has yet to be determined. Complex formation enhances dsRNA binding of the individual protomers."|complex-assembly:Heterotetramer|disease:"Severe acute respiratory syndrome (SARS) [EFO:0000694]: A viral respiratory infection caused by the SARS coronavirus. It is transmitted through close person-to-person contact. It is manifested with high fever, headache, dry cough and myalgias. It may progress to pneumonia and cause death."|complex-properties:"Primase complex without polymerase has a different stoichiometry (8:8). NSP12 possesses conserved motifs involved in template binding, NTP binding, and polymerization that assume a structure resembling a cupped “right hand”. The NSP7-NSP8 heterodimer binds to the index finger loop and may facilitates the interaction of NSP12 with additional components of the RNA synthesis machinery. The second NSP8 molecule binds close to the RNA template-binding channel." figure legend:Supplementary Table 1|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/10 - - - false - - 1 1 psi-mi:"MI:2287"(identification by structure determination) psi-mi:"MI:2287"(identification by structure determination) intact:EBI-26972469 uniprotkb:P0DTD1-PRO_0000449630 - intact:EBI-25475888 psi-mi:btc5_p_strand_40_mer(display_short)|psi-mi:EBI-26972469(display_long) psi-mi:p0dtd1-pro_0000449630(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0410"(3D electron microscopy) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972376|emdb:EMD-23007|imex:IM-28942-1|wwpdb:7krn - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - - chain-seq-start:5325|chain-seq-end:5925 figure legend:Supplementary Table 1|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/10 - rogid:F98KM8XJ6ElyhUel43fFsarYA3g2697049 - false - - 1 1 psi-mi:"MI:2287"(identification by structure determination) psi-mi:"MI:2287"(identification by structure determination) intact:EBI-26972469 intact:EBI-26972460 - - psi-mi:btc5_p_strand_40_mer(display_short)|psi-mi:EBI-26972469(display_long) psi-mi:rtc_t_strand_3'_nc_045512.2(display_short)|psi-mi:EBI-26972460(display_long) psi-mi:"MI:0410"(3D electron microscopy) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972376|emdb:EMD-23007|imex:IM-28942-1|wwpdb:7krn - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0320"(ribonucleic acid) - refseq:NC_045512.2(see-also) - - comment:NC_045512.2 residues 29831 - 29870 3' end figure legend:Supplementary Table 1|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/10 - - - false - - 1 1 psi-mi:"MI:2287"(identification by structure determination) psi-mi:"MI:2287"(identification by structure determination) intact:EBI-26972469 wwpdb:7ctt - intact:EBI-25564960|wwpdb:6xqb|emdb:EMD-22288|wwpdb:6M71|wwpdb:7btf|emdb:EMD-30178|emdb:EMD-30127|intenz:2.7.7.48|wwpdb:7bv2|emdb:EMD-30210|wwpdb:7bv1|emdb:EMD-30209|wwpdb:6x2g|wwpdb:7bw4|emdb:EMD-30226|wwpdb:7l1f|emdb:EMD-23109|wwpdb:6yyt|emdb:EMD-11007|wwpdb:7c2k|emdb:EMD-30275|wwpdb:7bzf|emdb:EMD-30252|reactome:R-COV-9691364|wwpdb:7aap|emdb:EMD-11692|wwpdb:7d4f|emdb:EMD-30572|emdb:EMD-23009|wwpdb:7krp|wwpdb:7b3b|emdb:EMD-11993|wwpdb:7b3c|emdb:EMD-11994|wwpdb:7b3d|emdb:EMD-11995|rhea:"RHEA:21248"|wwpdb:7ozv|emdb:EMD-13138|wwpdb:7ozu|emdb:EMD-13135|wwpdb:7oyg|emdb:EMD-13116 psi-mi:btc5_p_strand_40_mer(display_short)|psi-mi:EBI-26972469(display_long) psi-mi:nsp7-nsp8-nsp12_sars2(display_short)|psi-mi:7ctt(display_long)|intact:SARS-CoV-2 polymerase complex(complex recommended name)|intact:"NSP7:2xNSP8:NSP12"(complex systematic name)|intact:replication-transcription complex(complex synonym)|intact:replication and transcription complex(complex synonym)|intact:RTC(complex synonym)|intact:central RTC(complex synonym)|intact:C-RTC(complex synonym)|intact:holo-RdRp complex(complex synonym)|intact:holo-RNA-dependent RNA polymerase complex(complex synonym) psi-mi:"MI:0410"(3D electron microscopy) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972483|wwpdb:7krp|emdb:EMD-23009|imex:IM-28942-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:1302"(stable complex) - intact:EBI-25506373(exp-evidence)|pubmed:32277040(see-also)|go:"GO:0003968"(RNA-directed 5'-3' RNA polymerase activity)|go:"GO:0003725"(double-stranded RNA binding)|go:"GO:0039694"(viral RNA genome replication)|efo:"EFO:0000694"(see-also)|pubmed:16228002(see-also)|go:"GO:0031381"(viral RNA-directed RNA polymerase complex)|pubmed:19875418(see-also)|pubmed:31138817(see-also)|pubmed:31987001(see-also)|pubmed:18255185(see-also)|complex portal:CPX-5742(complex-primary)|pubmed:32015508(see-also)|evidence ontology:"ECO:0000353" - - curated-complex:"RNA-directed 5'-3' RNA polymerase of the SARS-CoV-2 coronavirus which consists of the main polymerase protein NSP12 and a stoichiometric variant of the primase complex (CPX-5690). Extends partially double-stranded RNA templates and is probably also required for transcription initiation, though the mechanism for this has yet to be determined. Complex formation enhances dsRNA binding of the individual protomers."|complex-assembly:Heterotetramer|disease:"Severe acute respiratory syndrome (SARS) [EFO:0000694]: A viral respiratory infection caused by the SARS coronavirus. It is transmitted through close person-to-person contact. It is manifested with high fever, headache, dry cough and myalgias. It may progress to pneumonia and cause death."|complex-properties:"Primase complex without polymerase has a different stoichiometry (8:8). NSP12 possesses conserved motifs involved in template binding, NTP binding, and polymerization that assume a structure resembling a cupped “right hand”. The NSP7-NSP8 heterodimer binds to the index finger loop and may facilitates the interaction of NSP12 with additional components of the RNA synthesis machinery. The second NSP8 molecule binds close to the RNA template-binding channel." figure legend:Supplementary Table 1|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/10 - - - false - - 1 1 psi-mi:"MI:2287"(identification by structure determination) psi-mi:"MI:2287"(identification by structure determination) intact:EBI-26972469 intact:EBI-26972460 - - psi-mi:btc5_p_strand_40_mer(display_short)|psi-mi:EBI-26972469(display_long) psi-mi:rtc_t_strand_3'_nc_045512.2(display_short)|psi-mi:EBI-26972460(display_long) psi-mi:"MI:0410"(3D electron microscopy) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972483|wwpdb:7krp|emdb:EMD-23009|imex:IM-28942-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0320"(ribonucleic acid) - refseq:NC_045512.2(see-also) - - comment:NC_045512.2 residues 29831 - 29870 3' end figure legend:Supplementary Table 1|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/10 - - - false - - 1 1 psi-mi:"MI:2287"(identification by structure determination) psi-mi:"MI:2287"(identification by structure determination) intact:EBI-26972469 wwpdb:7krn - intact:EBI-26617590|emdb:EMD-23007|wwpdb:7kro|emdb:EMD-23008|wwpdb:6xez|wwpdb:7cxm|wwpdb:7cxn|emdb:EMD-22160|emdb:EMD-30492|emdb:EMD-30493|intenz:2.7.7.48|rhea:"RHEA:13065"|intenz:3.6.4.13|rhea:"RHEA:21248"|intenz:2.7.7.50|rhea:"RHEA:54592"|rhea:"RHEA:60836"|rhea:"RHEA:60844" psi-mi:btc5_p_strand_40_mer(display_short)|psi-mi:EBI-26972469(display_long) psi-mi:rtc_sars2(display_short)|psi-mi:7krn(display_long)|intact:SARS-CoV-2 replication and transcription complex(complex recommended name)|intact:"NSP7:2xNSP8:NSP12:2xNSP13"(complex systematic name)|intact:mini RTC(complex synonym)|intact:RTC(complex synonym)|intact:replication-transcription complex(complex synonym)|intact:E-RTC(complex synonym)|intact:elongation RTC(complex synonym) psi-mi:"MI:0410"(3D electron microscopy) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972495|imex:IM-28942-5|wwpdb:7kro|emdb:EMD-23008 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:1302"(stable complex) - intact:EBI-25816865(exp-evidence)|pubmed:32277040(see-also)|go:"GO:0003968"(RNA-directed 5'-3' RNA polymerase activity)|go:"GO:0003725"(double-stranded RNA binding)|go:"GO:0039694"(viral RNA genome replication)|efo:"EFO:0000694"(see-also)|pubmed:16228002(see-also)|go:"GO:0031381"(viral RNA-directed RNA polymerase complex)|pubmed:19875418(see-also)|pubmed:31138817(see-also)|pubmed:31987001(see-also)|pubmed:18255185(see-also)|pubmed:32015508(see-also)|evidence ontology:"ECO:0000353"|complex portal:CPX-6442(complex-primary)|pubmed:33232691(see-also)|go:"GO:0070568"(guanylyltransferase activity)|go:"GO:0003724"(RNA helicase activity)|pubmed:32783916(see-also)|pubmed:33208736(see-also) - - curated-complex:"Replication and transcription complex (RTC) of the SARS-CoV-2 coronavirus which consists of the polymerase complex (CPX-5742) and 2 helicase molecules (nsp13). Although coronavirus nsp13s have been proposed to unwind RNA in the 5' to 3' direction, the 5' extension of template RNA is fed into the active site of SARS-CoV-2 nsp13 in the 3' to 5' direction. RNA polymerase has been known to possess a “backtrack” feature, in which the productive elongation and translocation complexes are in the same conformation to facilitate reversible backward motion during RNA synthesis. The SARS-CoV-2 RTC structure suggests it has the same function here. The nsp12 nucleotidyltransferase (NiRAN) domain possesses guanylyltransferase activity, catalyzing the formation of the cap core structure (GpppA) on the nascent mRNA. ADP-Mg2+ binds in the catalytic site of this domain."|complex-assembly:Heterohexamer|disease:"Severe acute respiratory syndrome (SARS) [EFO:0000694]: A viral respiratory infection caused by the SARS coronavirus. It is transmitted through close person-to-person contact. It is manifested with high fever, headache, dry cough and myalgias. It may progress to pneumonia and cause death."|complex-properties:"One of the nsp13 molecules stabilizes the overall architecture of the complex by interacting with both nsp7-nsp8-nsp12-RNA and the second nsp13 protein. This anchors the 5'-extension of RNA template, and enables continue transcription using the 5′ leader as a template, possibly by a second RTC complex. Orientation shifts of nsp13 results in its variable interactions with other components of this complex. While the second nsp13 molecule strongly binds to nsp12, the first makes only a weak hydrophobic contact. nsp9 monomer (P0DTD1-PRO_0000449627, not dimer CPX-5687) inhibits nsp12 guanylyltransferase activity." figure legend:Supplementary Table 1|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/10 - - - false - - 1 1 psi-mi:"MI:2287"(identification by structure determination) psi-mi:"MI:2287"(identification by structure determination) intact:EBI-26972469 intact:EBI-26972460 - - psi-mi:btc5_p_strand_40_mer(display_short)|psi-mi:EBI-26972469(display_long) psi-mi:rtc_t_strand_3'_nc_045512.2(display_short)|psi-mi:EBI-26972460(display_long) psi-mi:"MI:0410"(3D electron microscopy) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972495|imex:IM-28942-5|wwpdb:7kro|emdb:EMD-23008 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0320"(ribonucleic acid) - refseq:NC_045512.2(see-also) - - comment:NC_045512.2 residues 29831 - 29870 3' end figure legend:Supplementary Table 1|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/10 - - - false - - 1 1 psi-mi:"MI:2287"(identification by structure determination) psi-mi:"MI:2287"(identification by structure determination) intact:EBI-26972534 intact:EBI-26972460 - - psi-mi:p_strand_35_mer(display_short)|psi-mi:EBI-26972534(display_long) psi-mi:rtc_t_strand_3'_nc_045512.2(display_short)|psi-mi:EBI-26972460(display_long) psi-mi:"MI:0413"(electrophoretic mobility shift assay) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972520|imex:IM-28942-21 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0496"(bait) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0320"(ribonucleic acid) - refseq:NC_045512.2(see-also) - - comment:NC_045512.2 residues 29831 - 29870 3' end figure legend:1B, Lane 1, Supp. figure 1A lanes 1+3|comment:"The holo-RdRp (nsp7/nsp82/nsp12) bound the RTC scaffold in the presence or absence of nsp13 and ADP + AlF3."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/10 - - - false - - - - psi-mi:"MI:0816"(molecular weight estimation by staining) psi-mi:"MI:0816"(molecular weight estimation by staining) intact:EBI-26972534 wwpdb:7ctt - intact:EBI-25564960|wwpdb:6xqb|emdb:EMD-22288|wwpdb:6M71|wwpdb:7btf|emdb:EMD-30178|emdb:EMD-30127|intenz:2.7.7.48|wwpdb:7bv2|emdb:EMD-30210|wwpdb:7bv1|emdb:EMD-30209|wwpdb:6x2g|wwpdb:7bw4|emdb:EMD-30226|wwpdb:7l1f|emdb:EMD-23109|wwpdb:6yyt|emdb:EMD-11007|wwpdb:7c2k|emdb:EMD-30275|wwpdb:7bzf|emdb:EMD-30252|reactome:R-COV-9691364|wwpdb:7aap|emdb:EMD-11692|wwpdb:7d4f|emdb:EMD-30572|emdb:EMD-23009|wwpdb:7krp|wwpdb:7b3b|emdb:EMD-11993|wwpdb:7b3c|emdb:EMD-11994|wwpdb:7b3d|emdb:EMD-11995|rhea:"RHEA:21248"|wwpdb:7ozv|emdb:EMD-13138|wwpdb:7ozu|emdb:EMD-13135|wwpdb:7oyg|emdb:EMD-13116 psi-mi:p_strand_35_mer(display_short)|psi-mi:EBI-26972534(display_long) psi-mi:nsp7-nsp8-nsp12_sars2(display_short)|psi-mi:7ctt(display_long)|intact:SARS-CoV-2 polymerase complex(complex recommended name)|intact:"NSP7:2xNSP8:NSP12"(complex systematic name)|intact:replication-transcription complex(complex synonym)|intact:replication and transcription complex(complex synonym)|intact:RTC(complex synonym)|intact:central RTC(complex synonym)|intact:C-RTC(complex synonym)|intact:holo-RdRp complex(complex synonym)|intact:holo-RNA-dependent RNA polymerase complex(complex synonym) psi-mi:"MI:0413"(electrophoretic mobility shift assay) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972520|imex:IM-28942-21 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:1302"(stable complex) - intact:EBI-25506373(exp-evidence)|pubmed:32277040(see-also)|go:"GO:0003968"(RNA-directed 5'-3' RNA polymerase activity)|go:"GO:0003725"(double-stranded RNA binding)|go:"GO:0039694"(viral RNA genome replication)|efo:"EFO:0000694"(see-also)|pubmed:16228002(see-also)|go:"GO:0031381"(viral RNA-directed RNA polymerase complex)|pubmed:19875418(see-also)|pubmed:31138817(see-also)|pubmed:31987001(see-also)|pubmed:18255185(see-also)|complex portal:CPX-5742(complex-primary)|pubmed:32015508(see-also)|evidence ontology:"ECO:0000353" - - curated-complex:"RNA-directed 5'-3' RNA polymerase of the SARS-CoV-2 coronavirus which consists of the main polymerase protein NSP12 and a stoichiometric variant of the primase complex (CPX-5690). Extends partially double-stranded RNA templates and is probably also required for transcription initiation, though the mechanism for this has yet to be determined. Complex formation enhances dsRNA binding of the individual protomers."|complex-assembly:Heterotetramer|disease:"Severe acute respiratory syndrome (SARS) [EFO:0000694]: A viral respiratory infection caused by the SARS coronavirus. It is transmitted through close person-to-person contact. It is manifested with high fever, headache, dry cough and myalgias. It may progress to pneumonia and cause death."|complex-properties:"Primase complex without polymerase has a different stoichiometry (8:8). NSP12 possesses conserved motifs involved in template binding, NTP binding, and polymerization that assume a structure resembling a cupped “right hand”. The NSP7-NSP8 heterodimer binds to the index finger loop and may facilitates the interaction of NSP12 with additional components of the RNA synthesis machinery. The second NSP8 molecule binds close to the RNA template-binding channel." figure legend:1B, Lane 1, Supp. figure 1A lanes 1+3|comment:"The holo-RdRp (nsp7/nsp82/nsp12) bound the RTC scaffold in the presence or absence of nsp13 and ADP + AlF3."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/10 - - - false - mutation with no effect:760-760 - - psi-mi:"MI:0816"(molecular weight estimation by staining) psi-mi:"MI:0816"(molecular weight estimation by staining) intact:EBI-26972534 intact:EBI-26972460 - - psi-mi:p_strand_35_mer(display_short)|psi-mi:EBI-26972534(display_long) psi-mi:rtc_t_strand_3'_nc_045512.2(display_short)|psi-mi:EBI-26972460(display_long) psi-mi:"MI:0413"(electrophoretic mobility shift assay) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972527|imex:IM-28942-23 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0496"(bait) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0320"(ribonucleic acid) - refseq:NC_045512.2(see-also) - - comment:NC_045512.2 residues 29831 - 29870 3' end figure legend:1B, Lane 4, supp. figure 1A lanes 5 + 7|comment:"The holo-RdRp (nsp7/nsp82/nsp12) bound the RTC scaffold in the presence or absence of nsp13 and ADP + AlF3."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/11 - - - false - - - - psi-mi:"MI:0816"(molecular weight estimation by staining) psi-mi:"MI:0816"(molecular weight estimation by staining) intact:EBI-26972534 wwpdb:7krn - intact:EBI-26617590|emdb:EMD-23007|wwpdb:7kro|emdb:EMD-23008|wwpdb:6xez|wwpdb:7cxm|wwpdb:7cxn|emdb:EMD-22160|emdb:EMD-30492|emdb:EMD-30493|intenz:2.7.7.48|rhea:"RHEA:13065"|intenz:3.6.4.13|rhea:"RHEA:21248"|intenz:2.7.7.50|rhea:"RHEA:54592"|rhea:"RHEA:60836"|rhea:"RHEA:60844" psi-mi:p_strand_35_mer(display_short)|psi-mi:EBI-26972534(display_long) psi-mi:rtc_sars2(display_short)|psi-mi:7krn(display_long)|intact:SARS-CoV-2 replication and transcription complex(complex recommended name)|intact:"NSP7:2xNSP8:NSP12:2xNSP13"(complex systematic name)|intact:mini RTC(complex synonym)|intact:RTC(complex synonym)|intact:replication-transcription complex(complex synonym)|intact:E-RTC(complex synonym)|intact:elongation RTC(complex synonym) psi-mi:"MI:0413"(electrophoretic mobility shift assay) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972527|imex:IM-28942-23 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:1302"(stable complex) - intact:EBI-25816865(exp-evidence)|pubmed:32277040(see-also)|go:"GO:0003968"(RNA-directed 5'-3' RNA polymerase activity)|go:"GO:0003725"(double-stranded RNA binding)|go:"GO:0039694"(viral RNA genome replication)|efo:"EFO:0000694"(see-also)|pubmed:16228002(see-also)|go:"GO:0031381"(viral RNA-directed RNA polymerase complex)|pubmed:19875418(see-also)|pubmed:31138817(see-also)|pubmed:31987001(see-also)|pubmed:18255185(see-also)|pubmed:32015508(see-also)|evidence ontology:"ECO:0000353"|complex portal:CPX-6442(complex-primary)|pubmed:33232691(see-also)|go:"GO:0070568"(guanylyltransferase activity)|go:"GO:0003724"(RNA helicase activity)|pubmed:32783916(see-also)|pubmed:33208736(see-also) - - curated-complex:"Replication and transcription complex (RTC) of the SARS-CoV-2 coronavirus which consists of the polymerase complex (CPX-5742) and 2 helicase molecules (nsp13). Although coronavirus nsp13s have been proposed to unwind RNA in the 5' to 3' direction, the 5' extension of template RNA is fed into the active site of SARS-CoV-2 nsp13 in the 3' to 5' direction. RNA polymerase has been known to possess a “backtrack” feature, in which the productive elongation and translocation complexes are in the same conformation to facilitate reversible backward motion during RNA synthesis. The SARS-CoV-2 RTC structure suggests it has the same function here. The nsp12 nucleotidyltransferase (NiRAN) domain possesses guanylyltransferase activity, catalyzing the formation of the cap core structure (GpppA) on the nascent mRNA. ADP-Mg2+ binds in the catalytic site of this domain."|complex-assembly:Heterohexamer|disease:"Severe acute respiratory syndrome (SARS) [EFO:0000694]: A viral respiratory infection caused by the SARS coronavirus. It is transmitted through close person-to-person contact. It is manifested with high fever, headache, dry cough and myalgias. It may progress to pneumonia and cause death."|complex-properties:"One of the nsp13 molecules stabilizes the overall architecture of the complex by interacting with both nsp7-nsp8-nsp12-RNA and the second nsp13 protein. This anchors the 5'-extension of RNA template, and enables continue transcription using the 5′ leader as a template, possibly by a second RTC complex. Orientation shifts of nsp13 results in its variable interactions with other components of this complex. While the second nsp13 molecule strongly binds to nsp12, the first makes only a weak hydrophobic contact. nsp9 monomer (P0DTD1-PRO_0000449627, not dimer CPX-5687) inhibits nsp12 guanylyltransferase activity." figure legend:1B, Lane 4, supp. figure 1A lanes 5 + 7|comment:"The holo-RdRp (nsp7/nsp82/nsp12) bound the RTC scaffold in the presence or absence of nsp13 and ADP + AlF3."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/11 - - - false - mutation with no effect:760-760 - - psi-mi:"MI:0816"(molecular weight estimation by staining) psi-mi:"MI:0816"(molecular weight estimation by staining) intact:EBI-26972469 intact:EBI-26972460 - - psi-mi:btc5_p_strand_40_mer(display_short)|psi-mi:EBI-26972469(display_long) psi-mi:rtc_t_strand_3'_nc_045512.2(display_short)|psi-mi:EBI-26972460(display_long) psi-mi:"MI:0413"(electrophoretic mobility shift assay) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972540|imex:IM-28942-7 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0496"(bait) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0320"(ribonucleic acid) - refseq:NC_045512.2(see-also) - - comment:NC_045512.2 residues 29831 - 29870 3' end figure legend:1B, Lane 6, Supp. figure 1A lanes 6 + 8.|comment:Nsp13 was required for efficient binding to the BTC scaffolds.|comment:Interaction was seen in the presence of ADP + AlF3.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/10 - - - false - - - - psi-mi:"MI:0816"(molecular weight estimation by staining) psi-mi:"MI:0816"(molecular weight estimation by staining) intact:EBI-26972469 uniprotkb:P0DTD1-PRO_0000449630 - intact:EBI-25475888 psi-mi:btc5_p_strand_40_mer(display_short)|psi-mi:EBI-26972469(display_long) psi-mi:p0dtd1-pro_0000449630(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0413"(electrophoretic mobility shift assay) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972540|imex:IM-28942-7 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0684"(ancillary) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - - chain-seq-start:5325|chain-seq-end:5925 figure legend:1B, Lane 6, Supp. figure 1A lanes 6 + 8.|comment:Nsp13 was required for efficient binding to the BTC scaffolds.|comment:Interaction was seen in the presence of ADP + AlF3.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/10 - rogid:F98KM8XJ6ElyhUel43fFsarYA3g2697049 - false - - - - psi-mi:"MI:0816"(molecular weight estimation by staining) psi-mi:"MI:0816"(molecular weight estimation by staining) intact:EBI-26972469 wwpdb:7ctt - intact:EBI-25564960|wwpdb:6xqb|emdb:EMD-22288|wwpdb:6M71|wwpdb:7btf|emdb:EMD-30178|emdb:EMD-30127|intenz:2.7.7.48|wwpdb:7bv2|emdb:EMD-30210|wwpdb:7bv1|emdb:EMD-30209|wwpdb:6x2g|wwpdb:7bw4|emdb:EMD-30226|wwpdb:7l1f|emdb:EMD-23109|wwpdb:6yyt|emdb:EMD-11007|wwpdb:7c2k|emdb:EMD-30275|wwpdb:7bzf|emdb:EMD-30252|reactome:R-COV-9691364|wwpdb:7aap|emdb:EMD-11692|wwpdb:7d4f|emdb:EMD-30572|emdb:EMD-23009|wwpdb:7krp|wwpdb:7b3b|emdb:EMD-11993|wwpdb:7b3c|emdb:EMD-11994|wwpdb:7b3d|emdb:EMD-11995|rhea:"RHEA:21248"|wwpdb:7ozv|emdb:EMD-13138|wwpdb:7ozu|emdb:EMD-13135|wwpdb:7oyg|emdb:EMD-13116 psi-mi:btc5_p_strand_40_mer(display_short)|psi-mi:EBI-26972469(display_long) psi-mi:nsp7-nsp8-nsp12_sars2(display_short)|psi-mi:7ctt(display_long)|intact:SARS-CoV-2 polymerase complex(complex recommended name)|intact:"NSP7:2xNSP8:NSP12"(complex systematic name)|intact:replication-transcription complex(complex synonym)|intact:replication and transcription complex(complex synonym)|intact:RTC(complex synonym)|intact:central RTC(complex synonym)|intact:C-RTC(complex synonym)|intact:holo-RdRp complex(complex synonym)|intact:holo-RNA-dependent RNA polymerase complex(complex synonym) psi-mi:"MI:0413"(electrophoretic mobility shift assay) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972540|imex:IM-28942-7 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:1302"(stable complex) - intact:EBI-25506373(exp-evidence)|pubmed:32277040(see-also)|go:"GO:0003968"(RNA-directed 5'-3' RNA polymerase activity)|go:"GO:0003725"(double-stranded RNA binding)|go:"GO:0039694"(viral RNA genome replication)|efo:"EFO:0000694"(see-also)|pubmed:16228002(see-also)|go:"GO:0031381"(viral RNA-directed RNA polymerase complex)|pubmed:19875418(see-also)|pubmed:31138817(see-also)|pubmed:31987001(see-also)|pubmed:18255185(see-also)|complex portal:CPX-5742(complex-primary)|pubmed:32015508(see-also)|evidence ontology:"ECO:0000353" - - curated-complex:"RNA-directed 5'-3' RNA polymerase of the SARS-CoV-2 coronavirus which consists of the main polymerase protein NSP12 and a stoichiometric variant of the primase complex (CPX-5690). Extends partially double-stranded RNA templates and is probably also required for transcription initiation, though the mechanism for this has yet to be determined. Complex formation enhances dsRNA binding of the individual protomers."|complex-assembly:Heterotetramer|disease:"Severe acute respiratory syndrome (SARS) [EFO:0000694]: A viral respiratory infection caused by the SARS coronavirus. It is transmitted through close person-to-person contact. It is manifested with high fever, headache, dry cough and myalgias. It may progress to pneumonia and cause death."|complex-properties:"Primase complex without polymerase has a different stoichiometry (8:8). NSP12 possesses conserved motifs involved in template binding, NTP binding, and polymerization that assume a structure resembling a cupped “right hand”. The NSP7-NSP8 heterodimer binds to the index finger loop and may facilitates the interaction of NSP12 with additional components of the RNA synthesis machinery. The second NSP8 molecule binds close to the RNA template-binding channel." figure legend:1B, Lane 6, Supp. figure 1A lanes 6 + 8.|comment:Nsp13 was required for efficient binding to the BTC scaffolds.|comment:Interaction was seen in the presence of ADP + AlF3.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/10 - - - false - mutation with no effect:760-760 - - psi-mi:"MI:0816"(molecular weight estimation by staining) psi-mi:"MI:0816"(molecular weight estimation by staining) intact:EBI-26972588 intact:EBI-26972460 - - psi-mi:p_strand_38_mer(display_short)|psi-mi:EBI-26972588(display_long) psi-mi:rtc_t_strand_3'_nc_045512.2(display_short)|psi-mi:EBI-26972460(display_long) psi-mi:"MI:0413"(electrophoretic mobility shift assay) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972552|imex:IM-28942-9 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0496"(bait) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0320"(ribonucleic acid) - refseq:NC_045512.2(see-also) - - comment:NC_045512.2 residues 29831 - 29870 3' end figure legend:1B, Lane 5|comment:Interaction was seen in the presence of ADP + AlF3.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/10 - - - false - - - - psi-mi:"MI:0816"(molecular weight estimation by staining) psi-mi:"MI:0816"(molecular weight estimation by staining) intact:EBI-26972588 uniprotkb:P0DTD1-PRO_0000449630 - intact:EBI-25475888 psi-mi:p_strand_38_mer(display_short)|psi-mi:EBI-26972588(display_long) psi-mi:p0dtd1-pro_0000449630(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0413"(electrophoretic mobility shift assay) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972552|imex:IM-28942-9 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0684"(ancillary) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - - chain-seq-start:5325|chain-seq-end:5925 figure legend:1B, Lane 5|comment:Interaction was seen in the presence of ADP + AlF3.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/10 - rogid:F98KM8XJ6ElyhUel43fFsarYA3g2697049 - false - - - - psi-mi:"MI:0816"(molecular weight estimation by staining) psi-mi:"MI:0816"(molecular weight estimation by staining) intact:EBI-26972588 wwpdb:7ctt - intact:EBI-25564960|wwpdb:6xqb|emdb:EMD-22288|wwpdb:6M71|wwpdb:7btf|emdb:EMD-30178|emdb:EMD-30127|intenz:2.7.7.48|wwpdb:7bv2|emdb:EMD-30210|wwpdb:7bv1|emdb:EMD-30209|wwpdb:6x2g|wwpdb:7bw4|emdb:EMD-30226|wwpdb:7l1f|emdb:EMD-23109|wwpdb:6yyt|emdb:EMD-11007|wwpdb:7c2k|emdb:EMD-30275|wwpdb:7bzf|emdb:EMD-30252|reactome:R-COV-9691364|wwpdb:7aap|emdb:EMD-11692|wwpdb:7d4f|emdb:EMD-30572|emdb:EMD-23009|wwpdb:7krp|wwpdb:7b3b|emdb:EMD-11993|wwpdb:7b3c|emdb:EMD-11994|wwpdb:7b3d|emdb:EMD-11995|rhea:"RHEA:21248"|wwpdb:7ozv|emdb:EMD-13138|wwpdb:7ozu|emdb:EMD-13135|wwpdb:7oyg|emdb:EMD-13116 psi-mi:p_strand_38_mer(display_short)|psi-mi:EBI-26972588(display_long) psi-mi:nsp7-nsp8-nsp12_sars2(display_short)|psi-mi:7ctt(display_long)|intact:SARS-CoV-2 polymerase complex(complex recommended name)|intact:"NSP7:2xNSP8:NSP12"(complex systematic name)|intact:replication-transcription complex(complex synonym)|intact:replication and transcription complex(complex synonym)|intact:RTC(complex synonym)|intact:central RTC(complex synonym)|intact:C-RTC(complex synonym)|intact:holo-RdRp complex(complex synonym)|intact:holo-RNA-dependent RNA polymerase complex(complex synonym) psi-mi:"MI:0413"(electrophoretic mobility shift assay) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972552|imex:IM-28942-9 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:1302"(stable complex) - intact:EBI-25506373(exp-evidence)|pubmed:32277040(see-also)|go:"GO:0003968"(RNA-directed 5'-3' RNA polymerase activity)|go:"GO:0003725"(double-stranded RNA binding)|go:"GO:0039694"(viral RNA genome replication)|efo:"EFO:0000694"(see-also)|pubmed:16228002(see-also)|go:"GO:0031381"(viral RNA-directed RNA polymerase complex)|pubmed:19875418(see-also)|pubmed:31138817(see-also)|pubmed:31987001(see-also)|pubmed:18255185(see-also)|complex portal:CPX-5742(complex-primary)|pubmed:32015508(see-also)|evidence ontology:"ECO:0000353" - - curated-complex:"RNA-directed 5'-3' RNA polymerase of the SARS-CoV-2 coronavirus which consists of the main polymerase protein NSP12 and a stoichiometric variant of the primase complex (CPX-5690). Extends partially double-stranded RNA templates and is probably also required for transcription initiation, though the mechanism for this has yet to be determined. Complex formation enhances dsRNA binding of the individual protomers."|complex-assembly:Heterotetramer|disease:"Severe acute respiratory syndrome (SARS) [EFO:0000694]: A viral respiratory infection caused by the SARS coronavirus. It is transmitted through close person-to-person contact. It is manifested with high fever, headache, dry cough and myalgias. It may progress to pneumonia and cause death."|complex-properties:"Primase complex without polymerase has a different stoichiometry (8:8). NSP12 possesses conserved motifs involved in template binding, NTP binding, and polymerization that assume a structure resembling a cupped “right hand”. The NSP7-NSP8 heterodimer binds to the index finger loop and may facilitates the interaction of NSP12 with additional components of the RNA synthesis machinery. The second NSP8 molecule binds close to the RNA template-binding channel." figure legend:1B, Lane 5|comment:Interaction was seen in the presence of ADP + AlF3.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/10 - - - false - - - - psi-mi:"MI:0816"(molecular weight estimation by staining) psi-mi:"MI:0816"(molecular weight estimation by staining) intact:EBI-26972469 intact:EBI-26972460 - - psi-mi:btc5_p_strand_40_mer(display_short)|psi-mi:EBI-26972469(display_long) psi-mi:rtc_t_strand_3'_nc_045512.2(display_short)|psi-mi:EBI-26972460(display_long) psi-mi:"MI:0413"(electrophoretic mobility shift assay) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972592|imex:IM-28942-11 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0496"(bait) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0320"(ribonucleic acid) - refseq:NC_045512.2(see-also) - - comment:NC_045512.2 residues 29831 - 29870 3' end figure legend:Supp. figure 1A, lane 4|comment:The holo-RdRp with nsp12-D760A does not require nsp13 to bind the BTC5 scaffold.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/10 - - - false - - - - psi-mi:"MI:0816"(molecular weight estimation by staining) psi-mi:"MI:0816"(molecular weight estimation by staining) intact:EBI-26972469 wwpdb:7ctt - intact:EBI-25564960|wwpdb:6xqb|emdb:EMD-22288|wwpdb:6M71|wwpdb:7btf|emdb:EMD-30178|emdb:EMD-30127|intenz:2.7.7.48|wwpdb:7bv2|emdb:EMD-30210|wwpdb:7bv1|emdb:EMD-30209|wwpdb:6x2g|wwpdb:7bw4|emdb:EMD-30226|wwpdb:7l1f|emdb:EMD-23109|wwpdb:6yyt|emdb:EMD-11007|wwpdb:7c2k|emdb:EMD-30275|wwpdb:7bzf|emdb:EMD-30252|reactome:R-COV-9691364|wwpdb:7aap|emdb:EMD-11692|wwpdb:7d4f|emdb:EMD-30572|emdb:EMD-23009|wwpdb:7krp|wwpdb:7b3b|emdb:EMD-11993|wwpdb:7b3c|emdb:EMD-11994|wwpdb:7b3d|emdb:EMD-11995|rhea:"RHEA:21248"|wwpdb:7ozv|emdb:EMD-13138|wwpdb:7ozu|emdb:EMD-13135|wwpdb:7oyg|emdb:EMD-13116 psi-mi:btc5_p_strand_40_mer(display_short)|psi-mi:EBI-26972469(display_long) psi-mi:nsp7-nsp8-nsp12_sars2(display_short)|psi-mi:7ctt(display_long)|intact:SARS-CoV-2 polymerase complex(complex recommended name)|intact:"NSP7:2xNSP8:NSP12"(complex systematic name)|intact:replication-transcription complex(complex synonym)|intact:replication and transcription complex(complex synonym)|intact:RTC(complex synonym)|intact:central RTC(complex synonym)|intact:C-RTC(complex synonym)|intact:holo-RdRp complex(complex synonym)|intact:holo-RNA-dependent RNA polymerase complex(complex synonym) psi-mi:"MI:0413"(electrophoretic mobility shift assay) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972592|imex:IM-28942-11 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:1302"(stable complex) - intact:EBI-25506373(exp-evidence)|pubmed:32277040(see-also)|go:"GO:0003968"(RNA-directed 5'-3' RNA polymerase activity)|go:"GO:0003725"(double-stranded RNA binding)|go:"GO:0039694"(viral RNA genome replication)|efo:"EFO:0000694"(see-also)|pubmed:16228002(see-also)|go:"GO:0031381"(viral RNA-directed RNA polymerase complex)|pubmed:19875418(see-also)|pubmed:31138817(see-also)|pubmed:31987001(see-also)|pubmed:18255185(see-also)|complex portal:CPX-5742(complex-primary)|pubmed:32015508(see-also)|evidence ontology:"ECO:0000353" - - curated-complex:"RNA-directed 5'-3' RNA polymerase of the SARS-CoV-2 coronavirus which consists of the main polymerase protein NSP12 and a stoichiometric variant of the primase complex (CPX-5690). Extends partially double-stranded RNA templates and is probably also required for transcription initiation, though the mechanism for this has yet to be determined. Complex formation enhances dsRNA binding of the individual protomers."|complex-assembly:Heterotetramer|disease:"Severe acute respiratory syndrome (SARS) [EFO:0000694]: A viral respiratory infection caused by the SARS coronavirus. It is transmitted through close person-to-person contact. It is manifested with high fever, headache, dry cough and myalgias. It may progress to pneumonia and cause death."|complex-properties:"Primase complex without polymerase has a different stoichiometry (8:8). NSP12 possesses conserved motifs involved in template binding, NTP binding, and polymerization that assume a structure resembling a cupped “right hand”. The NSP7-NSP8 heterodimer binds to the index finger loop and may facilitates the interaction of NSP12 with additional components of the RNA synthesis machinery. The second NSP8 molecule binds close to the RNA template-binding channel." figure legend:Supp. figure 1A, lane 4|comment:The holo-RdRp with nsp12-D760A does not require nsp13 to bind the BTC5 scaffold.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/10 - - - false - mutation causing an interaction:760-760 - - psi-mi:"MI:0816"(molecular weight estimation by staining) psi-mi:"MI:0816"(molecular weight estimation by staining) intact:EBI-26972627 wwpdb:7ctt - intact:EBI-25564960|wwpdb:6xqb|emdb:EMD-22288|wwpdb:6M71|wwpdb:7btf|emdb:EMD-30178|emdb:EMD-30127|intenz:2.7.7.48|wwpdb:7bv2|emdb:EMD-30210|wwpdb:7bv1|emdb:EMD-30209|wwpdb:6x2g|wwpdb:7bw4|emdb:EMD-30226|wwpdb:7l1f|emdb:EMD-23109|wwpdb:6yyt|emdb:EMD-11007|wwpdb:7c2k|emdb:EMD-30275|wwpdb:7bzf|emdb:EMD-30252|reactome:R-COV-9691364|wwpdb:7aap|emdb:EMD-11692|wwpdb:7d4f|emdb:EMD-30572|emdb:EMD-23009|wwpdb:7krp|wwpdb:7b3b|emdb:EMD-11993|wwpdb:7b3c|emdb:EMD-11994|wwpdb:7b3d|emdb:EMD-11995|rhea:"RHEA:21248"|wwpdb:7ozv|emdb:EMD-13138|wwpdb:7ozu|emdb:EMD-13135|wwpdb:7oyg|emdb:EMD-13116 psi-mi:btc6_p_strand_41_mer(display_short)|psi-mi:EBI-26972627(display_long) psi-mi:nsp7-nsp8-nsp12_sars2(display_short)|psi-mi:7ctt(display_long)|intact:SARS-CoV-2 polymerase complex(complex recommended name)|intact:"NSP7:2xNSP8:NSP12"(complex systematic name)|intact:replication-transcription complex(complex synonym)|intact:replication and transcription complex(complex synonym)|intact:RTC(complex synonym)|intact:central RTC(complex synonym)|intact:C-RTC(complex synonym)|intact:holo-RdRp complex(complex synonym)|intact:holo-RNA-dependent RNA polymerase complex(complex synonym) psi-mi:"MI:0069"(mass spectrometry studies of complexes) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972614|imex:IM-28942-13 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:1302"(stable complex) - intact:EBI-25506373(exp-evidence)|pubmed:32277040(see-also)|go:"GO:0003968"(RNA-directed 5'-3' RNA polymerase activity)|go:"GO:0003725"(double-stranded RNA binding)|go:"GO:0039694"(viral RNA genome replication)|efo:"EFO:0000694"(see-also)|pubmed:16228002(see-also)|go:"GO:0031381"(viral RNA-directed RNA polymerase complex)|pubmed:19875418(see-also)|pubmed:31138817(see-also)|pubmed:31987001(see-also)|pubmed:18255185(see-also)|complex portal:CPX-5742(complex-primary)|pubmed:32015508(see-also)|evidence ontology:"ECO:0000353" - - curated-complex:"RNA-directed 5'-3' RNA polymerase of the SARS-CoV-2 coronavirus which consists of the main polymerase protein NSP12 and a stoichiometric variant of the primase complex (CPX-5690). Extends partially double-stranded RNA templates and is probably also required for transcription initiation, though the mechanism for this has yet to be determined. Complex formation enhances dsRNA binding of the individual protomers."|complex-assembly:Heterotetramer|disease:"Severe acute respiratory syndrome (SARS) [EFO:0000694]: A viral respiratory infection caused by the SARS coronavirus. It is transmitted through close person-to-person contact. It is manifested with high fever, headache, dry cough and myalgias. It may progress to pneumonia and cause death."|complex-properties:"Primase complex without polymerase has a different stoichiometry (8:8). NSP12 possesses conserved motifs involved in template binding, NTP binding, and polymerization that assume a structure resembling a cupped “right hand”. The NSP7-NSP8 heterodimer binds to the index finger loop and may facilitates the interaction of NSP12 with additional components of the RNA synthesis machinery. The second NSP8 molecule binds close to the RNA template-binding channel." figure legend:Supplementary Figure 1 B/C|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/10 - - - false - - 1 1 psi-mi:"MI:0082"(peptide massfingerprinting) psi-mi:"MI:0082"(peptide massfingerprinting) intact:EBI-26972627 uniprotkb:P0DTD1-PRO_0000449630 - intact:EBI-25475888 psi-mi:btc6_p_strand_41_mer(display_short)|psi-mi:EBI-26972627(display_long) psi-mi:p0dtd1-pro_0000449630(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0069"(mass spectrometry studies of complexes) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972614|imex:IM-28942-13 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - - chain-seq-start:5325|chain-seq-end:5925 figure legend:Supplementary Figure 1 B/C|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/10 - rogid:F98KM8XJ6ElyhUel43fFsarYA3g2697049 - false - - 1 1 psi-mi:"MI:0082"(peptide massfingerprinting) psi-mi:"MI:0082"(peptide massfingerprinting) intact:EBI-26972627 intact:EBI-26972460 - - psi-mi:btc6_p_strand_41_mer(display_short)|psi-mi:EBI-26972627(display_long) psi-mi:rtc_t_strand_3'_nc_045512.2(display_short)|psi-mi:EBI-26972460(display_long) psi-mi:"MI:0069"(mass spectrometry studies of complexes) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972614|imex:IM-28942-13 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0320"(ribonucleic acid) - refseq:NC_045512.2(see-also) - - comment:NC_045512.2 residues 29831 - 29870 3' end figure legend:Supplementary Figure 1 B/C|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/10 - - - false - - 1 1 psi-mi:"MI:0082"(peptide massfingerprinting) psi-mi:"MI:0082"(peptide massfingerprinting) intact:EBI-26972627 wwpdb:7krn - intact:EBI-26617590|emdb:EMD-23007|wwpdb:7kro|emdb:EMD-23008|wwpdb:6xez|wwpdb:7cxm|wwpdb:7cxn|emdb:EMD-22160|emdb:EMD-30492|emdb:EMD-30493|intenz:2.7.7.48|rhea:"RHEA:13065"|intenz:3.6.4.13|rhea:"RHEA:21248"|intenz:2.7.7.50|rhea:"RHEA:54592"|rhea:"RHEA:60836"|rhea:"RHEA:60844" psi-mi:btc6_p_strand_41_mer(display_short)|psi-mi:EBI-26972627(display_long) psi-mi:rtc_sars2(display_short)|psi-mi:7krn(display_long)|intact:SARS-CoV-2 replication and transcription complex(complex recommended name)|intact:"NSP7:2xNSP8:NSP12:2xNSP13"(complex systematic name)|intact:mini RTC(complex synonym)|intact:RTC(complex synonym)|intact:replication-transcription complex(complex synonym)|intact:E-RTC(complex synonym)|intact:elongation RTC(complex synonym) psi-mi:"MI:0069"(mass spectrometry studies of complexes) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972621|imex:IM-28942-15 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:1302"(stable complex) - intact:EBI-25816865(exp-evidence)|pubmed:32277040(see-also)|go:"GO:0003968"(RNA-directed 5'-3' RNA polymerase activity)|go:"GO:0003725"(double-stranded RNA binding)|go:"GO:0039694"(viral RNA genome replication)|efo:"EFO:0000694"(see-also)|pubmed:16228002(see-also)|go:"GO:0031381"(viral RNA-directed RNA polymerase complex)|pubmed:19875418(see-also)|pubmed:31138817(see-also)|pubmed:31987001(see-also)|pubmed:18255185(see-also)|pubmed:32015508(see-also)|evidence ontology:"ECO:0000353"|complex portal:CPX-6442(complex-primary)|pubmed:33232691(see-also)|go:"GO:0070568"(guanylyltransferase activity)|go:"GO:0003724"(RNA helicase activity)|pubmed:32783916(see-also)|pubmed:33208736(see-also) - - curated-complex:"Replication and transcription complex (RTC) of the SARS-CoV-2 coronavirus which consists of the polymerase complex (CPX-5742) and 2 helicase molecules (nsp13). Although coronavirus nsp13s have been proposed to unwind RNA in the 5' to 3' direction, the 5' extension of template RNA is fed into the active site of SARS-CoV-2 nsp13 in the 3' to 5' direction. RNA polymerase has been known to possess a “backtrack” feature, in which the productive elongation and translocation complexes are in the same conformation to facilitate reversible backward motion during RNA synthesis. The SARS-CoV-2 RTC structure suggests it has the same function here. The nsp12 nucleotidyltransferase (NiRAN) domain possesses guanylyltransferase activity, catalyzing the formation of the cap core structure (GpppA) on the nascent mRNA. ADP-Mg2+ binds in the catalytic site of this domain."|complex-assembly:Heterohexamer|disease:"Severe acute respiratory syndrome (SARS) [EFO:0000694]: A viral respiratory infection caused by the SARS coronavirus. It is transmitted through close person-to-person contact. It is manifested with high fever, headache, dry cough and myalgias. It may progress to pneumonia and cause death."|complex-properties:"One of the nsp13 molecules stabilizes the overall architecture of the complex by interacting with both nsp7-nsp8-nsp12-RNA and the second nsp13 protein. This anchors the 5'-extension of RNA template, and enables continue transcription using the 5′ leader as a template, possibly by a second RTC complex. Orientation shifts of nsp13 results in its variable interactions with other components of this complex. While the second nsp13 molecule strongly binds to nsp12, the first makes only a weak hydrophobic contact. nsp9 monomer (P0DTD1-PRO_0000449627, not dimer CPX-5687) inhibits nsp12 guanylyltransferase activity." figure legend:Supplementary figure 1 B/C|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/10 - - - false - - 1 1 psi-mi:"MI:0082"(peptide massfingerprinting) psi-mi:"MI:0082"(peptide massfingerprinting) intact:EBI-26972627 intact:EBI-26972460 - - psi-mi:btc6_p_strand_41_mer(display_short)|psi-mi:EBI-26972627(display_long) psi-mi:rtc_t_strand_3'_nc_045512.2(display_short)|psi-mi:EBI-26972460(display_long) psi-mi:"MI:0069"(mass spectrometry studies of complexes) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972621|imex:IM-28942-15 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0320"(ribonucleic acid) - refseq:NC_045512.2(see-also) - - comment:NC_045512.2 residues 29831 - 29870 3' end figure legend:Supplementary figure 1 B/C|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/10 - - - false - - 1 1 psi-mi:"MI:0082"(peptide massfingerprinting) psi-mi:"MI:0082"(peptide massfingerprinting) intact:EBI-26972534 intact:EBI-26972460 - - psi-mi:p_strand_35_mer(display_short)|psi-mi:EBI-26972534(display_long) psi-mi:rtc_t_strand_3'_nc_045512.2(display_short)|psi-mi:EBI-26972460(display_long) psi-mi:"MI:0030"(cross-linking study) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972643|imex:IM-28942-17 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0496"(bait) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0320"(ribonucleic acid) - refseq:NC_045512.2(see-also) - - comment:NC_045512.2 residues 29831 - 29870 3' end figure legend:Supp. figure 5A, lanes 1,4, S5B, Lane 1|comment:Interaction was seen in the presence of ATP and UV light.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/11 - - - false RNA chemical modification:34-34|32p radiolabel:1-1 - - - psi-mi:"MI:0821"(molecular weight estimation by autoradiography) psi-mi:"MI:0821"(molecular weight estimation by autoradiography) intact:EBI-26972534 wwpdb:7ctt - intact:EBI-25564960|wwpdb:6xqb|emdb:EMD-22288|wwpdb:6M71|wwpdb:7btf|emdb:EMD-30178|emdb:EMD-30127|intenz:2.7.7.48|wwpdb:7bv2|emdb:EMD-30210|wwpdb:7bv1|emdb:EMD-30209|wwpdb:6x2g|wwpdb:7bw4|emdb:EMD-30226|wwpdb:7l1f|emdb:EMD-23109|wwpdb:6yyt|emdb:EMD-11007|wwpdb:7c2k|emdb:EMD-30275|wwpdb:7bzf|emdb:EMD-30252|reactome:R-COV-9691364|wwpdb:7aap|emdb:EMD-11692|wwpdb:7d4f|emdb:EMD-30572|emdb:EMD-23009|wwpdb:7krp|wwpdb:7b3b|emdb:EMD-11993|wwpdb:7b3c|emdb:EMD-11994|wwpdb:7b3d|emdb:EMD-11995|rhea:"RHEA:21248"|wwpdb:7ozv|emdb:EMD-13138|wwpdb:7ozu|emdb:EMD-13135|wwpdb:7oyg|emdb:EMD-13116 psi-mi:p_strand_35_mer(display_short)|psi-mi:EBI-26972534(display_long) psi-mi:nsp7-nsp8-nsp12_sars2(display_short)|psi-mi:7ctt(display_long)|intact:SARS-CoV-2 polymerase complex(complex recommended name)|intact:"NSP7:2xNSP8:NSP12"(complex systematic name)|intact:replication-transcription complex(complex synonym)|intact:replication and transcription complex(complex synonym)|intact:RTC(complex synonym)|intact:central RTC(complex synonym)|intact:C-RTC(complex synonym)|intact:holo-RdRp complex(complex synonym)|intact:holo-RNA-dependent RNA polymerase complex(complex synonym) psi-mi:"MI:0030"(cross-linking study) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972643|imex:IM-28942-17 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:1302"(stable complex) - intact:EBI-25506373(exp-evidence)|pubmed:32277040(see-also)|go:"GO:0003968"(RNA-directed 5'-3' RNA polymerase activity)|go:"GO:0003725"(double-stranded RNA binding)|go:"GO:0039694"(viral RNA genome replication)|efo:"EFO:0000694"(see-also)|pubmed:16228002(see-also)|go:"GO:0031381"(viral RNA-directed RNA polymerase complex)|pubmed:19875418(see-also)|pubmed:31138817(see-also)|pubmed:31987001(see-also)|pubmed:18255185(see-also)|complex portal:CPX-5742(complex-primary)|pubmed:32015508(see-also)|evidence ontology:"ECO:0000353" - - curated-complex:"RNA-directed 5'-3' RNA polymerase of the SARS-CoV-2 coronavirus which consists of the main polymerase protein NSP12 and a stoichiometric variant of the primase complex (CPX-5690). Extends partially double-stranded RNA templates and is probably also required for transcription initiation, though the mechanism for this has yet to be determined. Complex formation enhances dsRNA binding of the individual protomers."|complex-assembly:Heterotetramer|disease:"Severe acute respiratory syndrome (SARS) [EFO:0000694]: A viral respiratory infection caused by the SARS coronavirus. It is transmitted through close person-to-person contact. It is manifested with high fever, headache, dry cough and myalgias. It may progress to pneumonia and cause death."|complex-properties:"Primase complex without polymerase has a different stoichiometry (8:8). NSP12 possesses conserved motifs involved in template binding, NTP binding, and polymerization that assume a structure resembling a cupped “right hand”. The NSP7-NSP8 heterodimer binds to the index finger loop and may facilitates the interaction of NSP12 with additional components of the RNA synthesis machinery. The second NSP8 molecule binds close to the RNA template-binding channel." figure legend:Supp. figure 5A, lanes 1,4, S5B, Lane 1|comment:Interaction was seen in the presence of ATP and UV light.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/11 - - - false RNA chemical modification:34-34|32p radiolabel:1-1 mutation increasing interaction strength:760-760 - - psi-mi:"MI:0821"(molecular weight estimation by autoradiography) psi-mi:"MI:0821"(molecular weight estimation by autoradiography) intact:EBI-26972534 intact:EBI-26972460 - - psi-mi:p_strand_35_mer(display_short)|psi-mi:EBI-26972534(display_long) psi-mi:rtc_t_strand_3'_nc_045512.2(display_short)|psi-mi:EBI-26972460(display_long) psi-mi:"MI:0030"(cross-linking study) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972651|imex:IM-28942-19 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0496"(bait) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0320"(ribonucleic acid) - refseq:NC_045512.2(see-also) - - comment:NC_045512.2 residues 29831 - 29870 3' end figure legend:"Supp. figure 5A, lanes 2,(3), 5-10, S5B, Lane 2"|comment:Interaction was seen in the and presence and absence of ATP and UV light, although reduced in the absence of UV. For the D760A mutant the absence of UV resulted in a much greater reduction in binding than for WT.|comment:Cross-linking of the p-RNA to nsp12 was substantially increased by the addition of nsp13 with ATP.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/11 - - - false RNA chemical modification:34-34|32p radiolabel:1-1 - - - psi-mi:"MI:0821"(molecular weight estimation by autoradiography) psi-mi:"MI:0821"(molecular weight estimation by autoradiography) intact:EBI-26972534 wwpdb:7krn - intact:EBI-26617590|emdb:EMD-23007|wwpdb:7kro|emdb:EMD-23008|wwpdb:6xez|wwpdb:7cxm|wwpdb:7cxn|emdb:EMD-22160|emdb:EMD-30492|emdb:EMD-30493|intenz:2.7.7.48|rhea:"RHEA:13065"|intenz:3.6.4.13|rhea:"RHEA:21248"|intenz:2.7.7.50|rhea:"RHEA:54592"|rhea:"RHEA:60836"|rhea:"RHEA:60844" psi-mi:p_strand_35_mer(display_short)|psi-mi:EBI-26972534(display_long) psi-mi:rtc_sars2(display_short)|psi-mi:7krn(display_long)|intact:SARS-CoV-2 replication and transcription complex(complex recommended name)|intact:"NSP7:2xNSP8:NSP12:2xNSP13"(complex systematic name)|intact:mini RTC(complex synonym)|intact:RTC(complex synonym)|intact:replication-transcription complex(complex synonym)|intact:E-RTC(complex synonym)|intact:elongation RTC(complex synonym) psi-mi:"MI:0030"(cross-linking study) Malone et al. (2021) pubmed:33883267|imex:IM-28942 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26972651|imex:IM-28942-19 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:1302"(stable complex) - intact:EBI-25816865(exp-evidence)|pubmed:32277040(see-also)|go:"GO:0003968"(RNA-directed 5'-3' RNA polymerase activity)|go:"GO:0003725"(double-stranded RNA binding)|go:"GO:0039694"(viral RNA genome replication)|efo:"EFO:0000694"(see-also)|pubmed:16228002(see-also)|go:"GO:0031381"(viral RNA-directed RNA polymerase complex)|pubmed:19875418(see-also)|pubmed:31138817(see-also)|pubmed:31987001(see-also)|pubmed:18255185(see-also)|pubmed:32015508(see-also)|evidence ontology:"ECO:0000353"|complex portal:CPX-6442(complex-primary)|pubmed:33232691(see-also)|go:"GO:0070568"(guanylyltransferase activity)|go:"GO:0003724"(RNA helicase activity)|pubmed:32783916(see-also)|pubmed:33208736(see-also) - - curated-complex:"Replication and transcription complex (RTC) of the SARS-CoV-2 coronavirus which consists of the polymerase complex (CPX-5742) and 2 helicase molecules (nsp13). Although coronavirus nsp13s have been proposed to unwind RNA in the 5' to 3' direction, the 5' extension of template RNA is fed into the active site of SARS-CoV-2 nsp13 in the 3' to 5' direction. RNA polymerase has been known to possess a “backtrack” feature, in which the productive elongation and translocation complexes are in the same conformation to facilitate reversible backward motion during RNA synthesis. The SARS-CoV-2 RTC structure suggests it has the same function here. The nsp12 nucleotidyltransferase (NiRAN) domain possesses guanylyltransferase activity, catalyzing the formation of the cap core structure (GpppA) on the nascent mRNA. ADP-Mg2+ binds in the catalytic site of this domain."|complex-assembly:Heterohexamer|disease:"Severe acute respiratory syndrome (SARS) [EFO:0000694]: A viral respiratory infection caused by the SARS coronavirus. It is transmitted through close person-to-person contact. It is manifested with high fever, headache, dry cough and myalgias. It may progress to pneumonia and cause death."|complex-properties:"One of the nsp13 molecules stabilizes the overall architecture of the complex by interacting with both nsp7-nsp8-nsp12-RNA and the second nsp13 protein. This anchors the 5'-extension of RNA template, and enables continue transcription using the 5′ leader as a template, possibly by a second RTC complex. Orientation shifts of nsp13 results in its variable interactions with other components of this complex. While the second nsp13 molecule strongly binds to nsp12, the first makes only a weak hydrophobic contact. nsp9 monomer (P0DTD1-PRO_0000449627, not dimer CPX-5687) inhibits nsp12 guanylyltransferase activity." figure legend:"Supp. figure 5A, lanes 2,(3), 5-10, S5B, Lane 2"|comment:Interaction was seen in the and presence and absence of ATP and UV light, although reduced in the absence of UV. For the D760A mutant the absence of UV resulted in a much greater reduction in binding than for WT.|comment:Cross-linking of the p-RNA to nsp12 was substantially increased by the addition of nsp13 with ATP.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/04/28 2021/05/11 - - - false RNA chemical modification:34-34|32p radiolabel:1-1 mutation increasing interaction strength:760-760 - - psi-mi:"MI:0821"(molecular weight estimation by autoradiography) psi-mi:"MI:0821"(molecular weight estimation by autoradiography)