#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q15828-PRO_0000006648	uniprotkb:Q99538-PRO_0000026502	intact:EBI-29014783	intact:EBI-29020361	psi-mi:q15828-pro_0000006648(display_long)|uniprotkb:CST6(gene name)|psi-mi:CST6(display_short)|uniprotkb:Cystatin-E(gene name synonym)|uniprotkb:Cystatin-6(gene name synonym)	psi-mi:q99538-pro_0000026502(display_long)|uniprotkb:Asparaginyl endopeptidase(gene name synonym)|uniprotkb:Protease, cysteine 1(gene name synonym)|uniprotkb:LGMN(gene name)|psi-mi:LGMN(display_short)|uniprotkb:PRSC1(gene name synonym)	psi-mi:"MI:0114"(x-ray crystallography)	Dall et al. (2015)	pubmed:25630877|imex:IM-29408	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-28964177|wwpdb:4N6O|imex:IM-29408-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	intact:EBI-7163527(chain-parent)	intact:EBI-2511794(chain-parent)	-	chain-seq-start:29|chain-seq-end:149	chain-seq-start:18|chain-seq-end:323	figure legend:Supp. Table 1|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/11/29	2022/01/18	rogid:5J+QcWmPpP40Ntkt4Eu3bUYEO9k9606	rogid:MF5hIURQx43Y8UHYKcoAmFEBbJ09606	rigid:xZbvgkCfBEJMOO1vTs7UqM+MW/0	false	his tag:c-c|fusion protein:n-n	sufficient binding region:9-286|mutation:246-246|mutation:130-130	1	1	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q15828-PRO_0000006648	uniprotkb:Q99538-PRO_0000026502	intact:EBI-29014783	intact:EBI-29020361	psi-mi:q15828-pro_0000006648(display_long)|uniprotkb:CST6(gene name)|psi-mi:CST6(display_short)|uniprotkb:Cystatin-E(gene name synonym)|uniprotkb:Cystatin-6(gene name synonym)	psi-mi:q99538-pro_0000026502(display_long)|uniprotkb:Asparaginyl endopeptidase(gene name synonym)|uniprotkb:Protease, cysteine 1(gene name synonym)|uniprotkb:LGMN(gene name)|psi-mi:LGMN(display_short)|uniprotkb:PRSC1(gene name synonym)	psi-mi:"MI:0114"(x-ray crystallography)	Dall et al. (2015)	pubmed:25630877|imex:IM-29408	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-28964252|wwpdb:4N6N|imex:IM-29408-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	intact:EBI-7163527(chain-parent)	intact:EBI-2511794(chain-parent)	-	chain-seq-start:29|chain-seq-end:149	chain-seq-start:18|chain-seq-end:323	figure legend:Supp. Table 1|comment:"Cys-172 of LGMN was oxidised by adding S-methyl methanethiosulfonate (MMTS), resulting in the mixed disulfide Cys172-S-CH3"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/11/29	2022/01/18	rogid:5J+QcWmPpP40Ntkt4Eu3bUYEO9k9606	rogid:MF5hIURQx43Y8UHYKcoAmFEBbJ09606	rigid:xZbvgkCfBEJMOO1vTs7UqM+MW/0	false	his tag:c-c|fusion protein:n-n	sufficient binding region:9-286|mutation:246-246|mutation:130-130|modified L-cysteine residue:172-172	1	1	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q99538-PRO_0000026502	chebi:"CHEBI:189000"	intact:EBI-29020361	intact:EBI-28964397	psi-mi:q99538-pro_0000026502(display_long)|uniprotkb:Asparaginyl endopeptidase(gene name synonym)|uniprotkb:Protease, cysteine 1(gene name synonym)|uniprotkb:LGMN(gene name)|psi-mi:LGMN(display_short)|uniprotkb:PRSC1(gene name synonym)	psi-mi:bz-asn-pna(display_short)|psi-mi:"CHEBI:189000"(display_long)	psi-mi:"MI:0435"(protease assay)	Dall et al. (2015)	pubmed:25630877|imex:IM-29408	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	psi-mi:"MI:0570"(protein cleavage)	psi-mi:"MI:0486"(UniProt)	intact:EBI-28964399|imex:IM-29408-5	-	-	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:1100"(bioactive entity)	intact:EBI-2511794(chain-parent)	-	-	chain-seq-start:18|chain-seq-end:323	-	figure legend:Fig. 2A, Supp. figure 2C|comment:AEP activity was lost at pH 6.5.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/11/29	2022/01/18	rogid:MF5hIURQx43Y8UHYKcoAmFEBbJ09606	-	-	false	sufficient binding region:9-286	sufficient binding region:9-285	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:189000"	uniprotkb:P01034-PRO_0000006639	intact:EBI-28964397	intact:EBI-29036734	psi-mi:bz-asn-pna(display_short)|psi-mi:"CHEBI:189000"(display_long)	psi-mi:p01034-pro_0000006639(display_long)|uniprotkb:Cystatin-3(gene name synonym)|uniprotkb:Gamma-trace(gene name synonym)|uniprotkb:Neuroendocrine basic polypeptide(gene name synonym)|uniprotkb:Post-gamma-globulin(gene name synonym)|uniprotkb:CST3(gene name)|psi-mi:CST3(display_short)	psi-mi:"MI:0435"(protease assay)	Dall et al. (2015)	pubmed:25630877|imex:IM-29408	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0570"(protein cleavage)	psi-mi:"MI:0486"(UniProt)	intact:EBI-28965665|imex:IM-29408-21	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0586"(inhibitor)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:1100"(bioactive entity)	psi-mi:"MI:0326"(protein)	-	intact:EBI-948622(chain-parent)	-	-	chain-seq-start:27|chain-seq-end:146	figure legend:Fig. 2A. Supp. figure 2D|comment:Inhibitory effect of CST3 observed at pH5.5 but not at pH4.0.  Mutating Asn-65 to Asp-65 enabled CST3 to inhibit at both pHs.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/11/29	2022/01/31	-	rogid:tkUZLEEhONKxhyOw2RooehXz0GI9606	-	false	-	fusion protein:n-n|his tag:c-c	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:189000"	uniprotkb:Q99538-PRO_0000026502	intact:EBI-28964397	intact:EBI-29020361	psi-mi:bz-asn-pna(display_short)|psi-mi:"CHEBI:189000"(display_long)	psi-mi:q99538-pro_0000026502(display_long)|uniprotkb:Asparaginyl endopeptidase(gene name synonym)|uniprotkb:Protease, cysteine 1(gene name synonym)|uniprotkb:LGMN(gene name)|psi-mi:LGMN(display_short)|uniprotkb:PRSC1(gene name synonym)	psi-mi:"MI:0435"(protease assay)	Dall et al. (2015)	pubmed:25630877|imex:IM-29408	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0570"(protein cleavage)	psi-mi:"MI:0486"(UniProt)	intact:EBI-28965665|imex:IM-29408-21	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:1100"(bioactive entity)	psi-mi:"MI:0326"(protein)	-	intact:EBI-2511794(chain-parent)	-	-	chain-seq-start:18|chain-seq-end:323	figure legend:Fig. 2A. Supp. figure 2D|comment:Inhibitory effect of CST3 observed at pH5.5 but not at pH4.0.  Mutating Asn-65 to Asp-65 enabled CST3 to inhibit at both pHs.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/11/29	2022/01/31	-	rogid:MF5hIURQx43Y8UHYKcoAmFEBbJ09606	-	false	-	mutation disrupting interaction rate:173-173|sufficient binding region:9-286	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:189000"	uniprotkb:Q15828-PRO_0000006648	intact:EBI-28964397	intact:EBI-29014783	psi-mi:bz-asn-pna(display_short)|psi-mi:"CHEBI:189000"(display_long)	psi-mi:q15828-pro_0000006648(display_long)|uniprotkb:CST6(gene name)|psi-mi:CST6(display_short)|uniprotkb:Cystatin-E(gene name synonym)|uniprotkb:Cystatin-6(gene name synonym)	psi-mi:"MI:0435"(protease assay)	Dall et al. (2015)	pubmed:25630877|imex:IM-29408	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0570"(protein cleavage)	psi-mi:"MI:0486"(UniProt)	intact:EBI-28965672|imex:IM-29408-7	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0586"(inhibitor)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:1100"(bioactive entity)	psi-mi:"MI:0326"(protein)	-	intact:EBI-7163527(chain-parent)	-	-	chain-seq-start:29|chain-seq-end:149	figure legend:Fig. 2A, Supp. Figs 2D,  5C|comment:Inhibitory effect of CST6 observed at pH5.5 and pH4.0|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/11/29	2022/01/18	-	rogid:5J+QcWmPpP40Ntkt4Eu3bUYEO9k9606	-	false	-	fusion protein:n-n|his tag:c-c|mutation increasing interaction rate:36-36	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:189000"	uniprotkb:Q99538-PRO_0000026502	intact:EBI-28964397	intact:EBI-29020361	psi-mi:bz-asn-pna(display_short)|psi-mi:"CHEBI:189000"(display_long)	psi-mi:q99538-pro_0000026502(display_long)|uniprotkb:Asparaginyl endopeptidase(gene name synonym)|uniprotkb:Protease, cysteine 1(gene name synonym)|uniprotkb:LGMN(gene name)|psi-mi:LGMN(display_short)|uniprotkb:PRSC1(gene name synonym)	psi-mi:"MI:0435"(protease assay)	Dall et al. (2015)	pubmed:25630877|imex:IM-29408	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0570"(protein cleavage)	psi-mi:"MI:0486"(UniProt)	intact:EBI-28965672|imex:IM-29408-7	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:1100"(bioactive entity)	psi-mi:"MI:0326"(protein)	-	intact:EBI-2511794(chain-parent)	-	-	chain-seq-start:18|chain-seq-end:323	figure legend:Fig. 2A, Supp. Figs 2D,  5C|comment:Inhibitory effect of CST6 observed at pH5.5 and pH4.0|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/11/29	2022/01/18	-	rogid:MF5hIURQx43Y8UHYKcoAmFEBbJ09606	-	false	-	mutation with no effect:173-173|sufficient binding region:9-286	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q15828-PRO_0000006648	uniprotkb:Q99538-PRO_0000026502	intact:EBI-29014783	intact:EBI-29020361	psi-mi:q15828-pro_0000006648(display_long)|uniprotkb:CST6(gene name)|psi-mi:CST6(display_short)|uniprotkb:Cystatin-E(gene name synonym)|uniprotkb:Cystatin-6(gene name synonym)	psi-mi:q99538-pro_0000026502(display_long)|uniprotkb:Asparaginyl endopeptidase(gene name synonym)|uniprotkb:Protease, cysteine 1(gene name synonym)|uniprotkb:LGMN(gene name)|psi-mi:LGMN(display_short)|uniprotkb:PRSC1(gene name synonym)	psi-mi:"MI:0071"(molecular sieving)	Dall et al. (2015)	pubmed:25630877|imex:IM-29408	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-28965695|imex:IM-29408-9	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	intact:EBI-7163527(chain-parent)	intact:EBI-2511794(chain-parent)	-	chain-seq-start:29|chain-seq-end:149	chain-seq-start:18|chain-seq-end:323	figure legend:Fig. 2B|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/11/29	2022/01/18	rogid:5J+QcWmPpP40Ntkt4Eu3bUYEO9k9606	rogid:MF5hIURQx43Y8UHYKcoAmFEBbJ09606	rigid:xZbvgkCfBEJMOO1vTs7UqM+MW/0	false	sufficient binding region:37-121	sufficient binding region:9-286	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:P01034-PRO_0000006639	uniprotkb:Q99538-PRO_0000026502	intact:EBI-29036734	intact:EBI-29020361	psi-mi:p01034-pro_0000006639(display_long)|uniprotkb:Cystatin-3(gene name synonym)|uniprotkb:Gamma-trace(gene name synonym)|uniprotkb:Neuroendocrine basic polypeptide(gene name synonym)|uniprotkb:Post-gamma-globulin(gene name synonym)|uniprotkb:CST3(gene name)|psi-mi:CST3(display_short)	psi-mi:q99538-pro_0000026502(display_long)|uniprotkb:Asparaginyl endopeptidase(gene name synonym)|uniprotkb:Protease, cysteine 1(gene name synonym)|uniprotkb:LGMN(gene name)|psi-mi:LGMN(display_short)|uniprotkb:PRSC1(gene name synonym)	psi-mi:"MI:0071"(molecular sieving)	Dall et al. (2015)	pubmed:25630877|imex:IM-29408	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-28965962|imex:IM-29408-13	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	intact:EBI-948622(chain-parent)	intact:EBI-2511794(chain-parent)	-	chain-seq-start:27|chain-seq-end:146	chain-seq-start:18|chain-seq-end:323	figure legend:Supp Fig. 3A|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/11/29	2022/01/31	rogid:tkUZLEEhONKxhyOw2RooehXz0GI9606	rogid:MF5hIURQx43Y8UHYKcoAmFEBbJ09606	rigid:TyrEPQpMOH86K+gh4h3RukyEYlM	false	sufficient binding region:40-120	sufficient binding region:9-286	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:Q15828	uniprotkb:Q99538-PRO_0000026502	intact:EBI-7163527|ensembl:ENSP00000311313|intact:MINT-8247486|uniprotkb:Q540N7	intact:EBI-29020361	psi-mi:cytm_human(display_long)|uniprotkb:CST6(gene name)|psi-mi:CST6(display_short)|uniprotkb:Cystatin-6(gene name synonym)|uniprotkb:Cystatin-E(gene name synonym)	psi-mi:q99538-pro_0000026502(display_long)|uniprotkb:Asparaginyl endopeptidase(gene name synonym)|uniprotkb:Protease, cysteine 1(gene name synonym)|uniprotkb:LGMN(gene name)|psi-mi:LGMN(display_short)|uniprotkb:PRSC1(gene name synonym)	psi-mi:"MI:0435"(protease assay)	Dall et al. (2015)	pubmed:25630877|imex:IM-29408	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0570"(protein cleavage)	psi-mi:"MI:0486"(UniProt)	intact:EBI-28965711|imex:IM-29408-23	-	-	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensembl:ENSG00000175315(gene)|ensembl:ENST00000312134(transcript)|go:"GO:0001533"(cornified envelope)|go:"GO:0004869"(cysteine-type endopeptidase inhibitor activity)|go:"GO:0008544"(epidermis development)|go:"GO:0009653"(anatomical structure morphogenesis)|go:"GO:0070062"(extracellular exosome)|interpro:IPR000010(Proteinase inhibitor I25, cystatin)|interpro:IPR018073|rcsb pdb:4N6L|rcsb pdb:4N6M|rcsb pdb:4N6N|rcsb pdb:4N6O|rcsb pdb:6FK0|refseq:NP_001314.1	intact:EBI-2511794(chain-parent)	-	comment:mint|comment:homomint	chain-seq-start:18|chain-seq-end:323	figure legend:Figs. 2C, 3|comment:The cleavage was favored at more acidic pH, with complete cleavage at pH4. At pH6 the CST6 peptide bond was religated by LGMN.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/11/29	2022/01/18	rogid:jHDap5wSSZ+/3uyliVh1ZgqexOw9606	rogid:MF5hIURQx43Y8UHYKcoAmFEBbJ09606	rigid:XPKvaSCeLqIOKfAkb7Sog3Ipw2U	false	his tag:c-c	mutation with no effect:130-130|mutation with no effect:130-130	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:P01040	uniprotkb:Q99538	intact:EBI-724303|ensembl:ENSP00000264474|uniprotkb:Q6IB90	intact:EBI-2511794|ensembl:ENSP00000334052|ensembl:ENSP00000376911|uniprotkb:Q86TV2|uniprotkb:Q86TV3|uniprotkb:Q9BTY1|uniprotkb:O00123|intact:EBI-10963477	psi-mi:cyta_human(display_long)|uniprotkb:Cystatin-AS(gene name synonym)|uniprotkb:Stefin-A(gene name synonym)|uniprotkb:CSTA(gene name)|psi-mi:CSTA(display_short)|uniprotkb:STF1(gene name synonym)|uniprotkb:STFA(gene name synonym)	psi-mi:lgmn_human(display_long)|uniprotkb:LGMN(gene name)|psi-mi:LGMN(display_short)|uniprotkb:PRSC1(gene name synonym)|uniprotkb:Asparaginyl endopeptidase(gene name synonym)|uniprotkb:Protease, cysteine 1(gene name synonym)	psi-mi:"MI:0435"(protease assay)	Dall et al. (2015)	pubmed:25630877|imex:IM-29408	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0570"(protein cleavage)	psi-mi:"MI:0486"(UniProt)	intact:EBI-28965976|imex:IM-29408-15	-	-	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_005204.1|go:"GO:0098609"(cell-cell adhesion)|interpro:IPR000010(Proteinase inhibitor I25, cystatin)|interpro:IPR001713(Proteinase inhibitor I25A, stefin A)|mint:P01040|rcsb pdb:1CYU|rcsb pdb:1CYV|rcsb pdb:1DVC|rcsb pdb:1DVD|rcsb pdb:1GD3|rcsb pdb:1GD4|rcsb pdb:1N9J|rcsb pdb:1NB3|rcsb pdb:1NB5|rcsb pdb:3K9M|rcsb pdb:3KFQ|interpro:IPR018073|rcsb pdb:3KSE|reactome:R-HSA-6809371|ensembl:ENSG00000121552(gene)|ensembl:ENST00000264474(transcript)|go:"GO:0001533"(cornified envelope)|go:"GO:0002020"(protease binding)|go:"GO:0004869"(cysteine-type endopeptidase inhibitor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0010466"(negative regulation of peptidase activity)|go:"GO:0018149"(peptide cross-linking)|go:"GO:0030216"(keratinocyte differentiation)|go:"GO:0045861"(negative regulation of proteolysis)	ensembl:ENSG00000100600(gene)|ensembl:ENST00000334869(transcript)|ensembl:ENST00000393218(transcript)|go:"GO:0003014"(renal system process)|go:"GO:0004197"(cysteine-type endopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005737"(cytoplasm)|go:"GO:0005764"(lysosome)|go:"GO:0005770"(late endosome)|go:"GO:0006508"(proteolysis)|go:"GO:0006624"(vacuolar protein processing)|go:"GO:0007613"(memory)|go:"GO:0008233"(peptidase activity)|go:"GO:0008284"(positive regulation of cell population proliferation)|go:"GO:0008306"(associative learning)|go:"GO:0010447"(response to acidic pH)|go:"GO:0010629"(negative regulation of gene expression)|go:"GO:0019886"(antigen processing and presentation of exogenous peptide antigen via MHC class II)|go:"GO:0032801"(receptor catabolic process)|go:"GO:0035729"(cellular response to hepatocyte growth factor stimulus)|go:"GO:0036021"(endolysosome lumen)|go:"GO:0042359"(vitamin D metabolic process)|go:"GO:0043202"(lysosomal lumen)|go:"GO:0043524"(negative regulation of neuron apoptotic process)|go:"GO:0045177"(apical part of cell)|go:"GO:0045931"(positive regulation of mitotic cell cycle)|go:"GO:0048156"(tau protein binding)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0051603"(proteolysis involved in cellular protein catabolic process)|go:"GO:0070062"(extracellular exosome)|go:"GO:0071277"(cellular response to calcium ion)|go:"GO:0090026"(positive regulation of monocyte chemotaxis)|go:"GO:0097061"(dendritic spine organization)|go:"GO:0097202"(activation of cysteine-type endopeptidase activity)|go:"GO:0097264"(self proteolysis)|go:"GO:1900273"(positive regulation of long-term synaptic potentiation)|go:"GO:1901185"(negative regulation of ERBB signaling pathway)|go:"GO:1904646"(cellular response to amyloid-beta)|go:"GO:2001028"(positive regulation of endothelial cell chemotaxis)|interpro:IPR001096(Peptidase C13, legumain)|interpro:IPR043577|mint:Q99538|rcsb pdb:4AW9|rcsb pdb:4AWA|rcsb pdb:4AWB|rcsb pdb:4FGU|rcsb pdb:4N6N|rcsb pdb:4N6O|rcsb pdb:5LU8|rcsb pdb:5LU9|rcsb pdb:5LUA|rcsb pdb:5LUB|reactome:R-HSA-1679131|go:"GO:0040015"(negative regulation of multicellular organism growth)|reactome:R-HSA-196791|reactome:R-HSA-2132295|refseq:XP_005267919.1|refseq:XP_005267920.1|refseq:NP_001008530.1|refseq:NP_005597.3|refseq:XP_016876952.1|refseq:XP_016876953.1|refseq:XP_016876954.1|refseq:XP_016876955.1|refseq:XP_016876956.1	-	-	-	figure legend:Supp. Fig.3B|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/11/29	2022/01/11	rogid:SzbpyWUHlOtItIvffzuwhcqFsDI9606	rogid:W2jPq8/peH5iXt1wmbm8lFnkJwM9606	rigid:IZQJ2QmNAIkJ6aXGG42tqmsPLAY	false	his tag:n-n	-	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:P04080	uniprotkb:Q99538	intact:EBI-1051966|ensembl:ENSP00000291568	intact:EBI-2511794|ensembl:ENSP00000334052|ensembl:ENSP00000376911|uniprotkb:Q86TV2|uniprotkb:Q86TV3|uniprotkb:Q9BTY1|uniprotkb:O00123|intact:EBI-10963477	psi-mi:cytb_human(display_long)|uniprotkb:Stefin-B(gene name synonym)|uniprotkb:Liver thiol proteinase inhibitor(gene name synonym)|uniprotkb:CPI-B(gene name synonym)|uniprotkb:CSTB(gene name)|psi-mi:CSTB(display_short)|uniprotkb:CST6(gene name synonym)|uniprotkb:STFB(gene name synonym)	psi-mi:lgmn_human(display_long)|uniprotkb:LGMN(gene name)|psi-mi:LGMN(display_short)|uniprotkb:PRSC1(gene name synonym)|uniprotkb:Asparaginyl endopeptidase(gene name synonym)|uniprotkb:Protease, cysteine 1(gene name synonym)	psi-mi:"MI:0435"(protease assay)	Dall et al. (2015)	pubmed:25630877|imex:IM-29408	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0570"(protein cleavage)	psi-mi:"MI:0486"(UniProt)	intact:EBI-28965990|imex:IM-29408-17	-	-	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_000091.1|ensembl:ENSG00000160213(gene)|ensembl:ENST00000291568(transcript)|go:"GO:0002020"(protease binding)|go:"GO:0003723"(RNA binding)|go:"GO:0004866"(endopeptidase inhibitor activity)|go:"GO:0004869"(cysteine-type endopeptidase inhibitor activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0005634"(nucleus)|go:"GO:0005730"(nucleolus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0008344"(adult locomotory behavior)|go:"GO:0010466"(negative regulation of peptidase activity)|go:"GO:0034774"(secretory granule lumen)|go:"GO:0045861"(negative regulation of proteolysis)|go:"GO:0062023"(collagen-containing extracellular matrix)|go:"GO:0070062"(extracellular exosome)|go:"GO:1904724"(tertiary granule lumen)|go:"GO:1904813"(ficolin-1-rich granule lumen)|interpro:IPR000010(Proteinase inhibitor I25, cystatin)|interpro:IPR001713(Proteinase inhibitor I25A, stefin A)|interpro:IPR018073|mint:P04080|rcsb pdb:1STF|rcsb pdb:2OCT|rcsb pdb:4N6V|reactome:R-HSA-6798695	ensembl:ENSG00000100600(gene)|ensembl:ENST00000334869(transcript)|ensembl:ENST00000393218(transcript)|go:"GO:0003014"(renal system process)|go:"GO:0004197"(cysteine-type endopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005737"(cytoplasm)|go:"GO:0005764"(lysosome)|go:"GO:0005770"(late endosome)|go:"GO:0006508"(proteolysis)|go:"GO:0006624"(vacuolar protein processing)|go:"GO:0007613"(memory)|go:"GO:0008233"(peptidase activity)|go:"GO:0008284"(positive regulation of cell population proliferation)|go:"GO:0008306"(associative learning)|go:"GO:0010447"(response to acidic pH)|go:"GO:0010629"(negative regulation of gene expression)|go:"GO:0019886"(antigen processing and presentation of exogenous peptide antigen via MHC class II)|go:"GO:0032801"(receptor catabolic process)|go:"GO:0035729"(cellular response to hepatocyte growth factor stimulus)|go:"GO:0036021"(endolysosome lumen)|go:"GO:0042359"(vitamin D metabolic process)|go:"GO:0043202"(lysosomal lumen)|go:"GO:0043524"(negative regulation of neuron apoptotic process)|go:"GO:0045177"(apical part of cell)|go:"GO:0045931"(positive regulation of mitotic cell cycle)|go:"GO:0048156"(tau protein binding)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0051603"(proteolysis involved in cellular protein catabolic process)|go:"GO:0070062"(extracellular exosome)|go:"GO:0071277"(cellular response to calcium ion)|go:"GO:0090026"(positive regulation of monocyte chemotaxis)|go:"GO:0097061"(dendritic spine organization)|go:"GO:0097202"(activation of cysteine-type endopeptidase activity)|go:"GO:0097264"(self proteolysis)|go:"GO:1900273"(positive regulation of long-term synaptic potentiation)|go:"GO:1901185"(negative regulation of ERBB signaling pathway)|go:"GO:1904646"(cellular response to amyloid-beta)|go:"GO:2001028"(positive regulation of endothelial cell chemotaxis)|interpro:IPR001096(Peptidase C13, legumain)|interpro:IPR043577|mint:Q99538|rcsb pdb:4AW9|rcsb pdb:4AWA|rcsb pdb:4AWB|rcsb pdb:4FGU|rcsb pdb:4N6N|rcsb pdb:4N6O|rcsb pdb:5LU8|rcsb pdb:5LU9|rcsb pdb:5LUA|rcsb pdb:5LUB|reactome:R-HSA-1679131|go:"GO:0040015"(negative regulation of multicellular organism growth)|reactome:R-HSA-196791|reactome:R-HSA-2132295|refseq:XP_005267919.1|refseq:XP_005267920.1|refseq:NP_001008530.1|refseq:NP_005597.3|refseq:XP_016876952.1|refseq:XP_016876953.1|refseq:XP_016876954.1|refseq:XP_016876955.1|refseq:XP_016876956.1	-	-	-	figure legend:Supp. Fig.3B|comment:Mass spectrometric analysis revealed processing after Asn-61.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/11/29	2022/01/11	rogid:KwR0XgOq6SeHdqxriNE8xIUMmUo9606	rogid:W2jPq8/peH5iXt1wmbm8lFnkJwM9606	rigid:uHvMMwNheeycW7/X39hDifFpG8w	false	his tag:n-n	-	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:P01034-PRO_0000006639	uniprotkb:Q99538-PRO_0000026502	intact:EBI-29036734	intact:EBI-29020361	psi-mi:p01034-pro_0000006639(display_long)|uniprotkb:Cystatin-3(gene name synonym)|uniprotkb:Gamma-trace(gene name synonym)|uniprotkb:Neuroendocrine basic polypeptide(gene name synonym)|uniprotkb:Post-gamma-globulin(gene name synonym)|uniprotkb:CST3(gene name)|psi-mi:CST3(display_short)	psi-mi:q99538-pro_0000026502(display_long)|uniprotkb:Asparaginyl endopeptidase(gene name synonym)|uniprotkb:Protease, cysteine 1(gene name synonym)|uniprotkb:LGMN(gene name)|psi-mi:LGMN(display_short)|uniprotkb:PRSC1(gene name synonym)	psi-mi:"MI:0435"(protease assay)	Dall et al. (2015)	pubmed:25630877|imex:IM-29408	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0570"(protein cleavage)	psi-mi:"MI:0486"(UniProt)	intact:EBI-28966017|imex:IM-29408-19	-	-	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	intact:EBI-948622(chain-parent)	intact:EBI-2511794(chain-parent)	-	chain-seq-start:27|chain-seq-end:146	chain-seq-start:18|chain-seq-end:323	figure legend:Supp. Figs. 4A, 4B|comment:"Cys-172 of LGMN was oxidised by adding S-methyl methanethiosulfonate (MMTS), resulting in the mixed disulfide Cys172-S-CH3"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/11/29	2022/01/18	rogid:tkUZLEEhONKxhyOw2RooehXz0GI9606	rogid:MF5hIURQx43Y8UHYKcoAmFEBbJ09606	rigid:TyrEPQpMOH86K+gh4h3RukyEYlM	false	his tag:c-c	modified L-cysteine residue:172-172	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:Q99538	-	intact:EBI-2511794|ensembl:ENSP00000334052|ensembl:ENSP00000376911|uniprotkb:Q86TV2|uniprotkb:Q86TV3|uniprotkb:Q9BTY1|uniprotkb:O00123|intact:EBI-10963477	-	psi-mi:lgmn_human(display_long)|uniprotkb:LGMN(gene name)|psi-mi:LGMN(display_short)|uniprotkb:PRSC1(gene name synonym)|uniprotkb:Asparaginyl endopeptidase(gene name synonym)|uniprotkb:Protease, cysteine 1(gene name synonym)	-	psi-mi:"MI:0435"(protease assay)	Dall et al. (2015)	pubmed:25630877|imex:IM-29408	taxid:9606(human)|taxid:9606(Homo sapiens)	-	psi-mi:"MI:0414"(enzymatic reaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-28966058|imex:IM-29408-33	-	-	psi-mi:"MI:0898"(putative self)	-	psi-mi:"MI:0898"(putative self)	-	psi-mi:"MI:0326"(protein)	-	ensembl:ENSG00000100600(gene)|ensembl:ENST00000334869(transcript)|ensembl:ENST00000393218(transcript)|go:"GO:0003014"(renal system process)|go:"GO:0004197"(cysteine-type endopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005737"(cytoplasm)|go:"GO:0005764"(lysosome)|go:"GO:0005770"(late endosome)|go:"GO:0006508"(proteolysis)|go:"GO:0006624"(vacuolar protein processing)|go:"GO:0007613"(memory)|go:"GO:0008233"(peptidase activity)|go:"GO:0008284"(positive regulation of cell population proliferation)|go:"GO:0008306"(associative learning)|go:"GO:0010447"(response to acidic pH)|go:"GO:0010629"(negative regulation of gene expression)|go:"GO:0019886"(antigen processing and presentation of exogenous peptide antigen via MHC class II)|go:"GO:0032801"(receptor catabolic process)|go:"GO:0035729"(cellular response to hepatocyte growth factor stimulus)|go:"GO:0036021"(endolysosome lumen)|go:"GO:0042359"(vitamin D metabolic process)|go:"GO:0043202"(lysosomal lumen)|go:"GO:0043524"(negative regulation of neuron apoptotic process)|go:"GO:0045177"(apical part of cell)|go:"GO:0045931"(positive regulation of mitotic cell cycle)|go:"GO:0048156"(tau protein binding)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0051603"(proteolysis involved in cellular protein catabolic process)|go:"GO:0070062"(extracellular exosome)|go:"GO:0071277"(cellular response to calcium ion)|go:"GO:0090026"(positive regulation of monocyte chemotaxis)|go:"GO:0097061"(dendritic spine organization)|go:"GO:0097202"(activation of cysteine-type endopeptidase activity)|go:"GO:0097264"(self proteolysis)|go:"GO:1900273"(positive regulation of long-term synaptic potentiation)|go:"GO:1901185"(negative regulation of ERBB signaling pathway)|go:"GO:1904646"(cellular response to amyloid-beta)|go:"GO:2001028"(positive regulation of endothelial cell chemotaxis)|interpro:IPR001096(Peptidase C13, legumain)|interpro:IPR043577|mint:Q99538|rcsb pdb:4AW9|rcsb pdb:4AWA|rcsb pdb:4AWB|rcsb pdb:4FGU|rcsb pdb:4N6N|rcsb pdb:4N6O|rcsb pdb:5LU8|rcsb pdb:5LU9|rcsb pdb:5LUA|rcsb pdb:5LUB|reactome:R-HSA-1679131|go:"GO:0040015"(negative regulation of multicellular organism growth)|reactome:R-HSA-196791|reactome:R-HSA-2132295|refseq:XP_005267919.1|refseq:XP_005267920.1|refseq:NP_001008530.1|refseq:NP_005597.3|refseq:XP_016876952.1|refseq:XP_016876953.1|refseq:XP_016876954.1|refseq:XP_016876955.1|refseq:XP_016876956.1	-	-	-	-	figure legend:Supp. Fig 4C|comment:At pH6 a cleaved CST6 peptide bond was religated by LGMN.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/11/29	2022/01/11	rogid:W2jPq8/peH5iXt1wmbm8lFnkJwM9606	-	-	false	modified L-cysteine residue:189-189|mutation:309-309,303-303	-	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	-
uniprotkb:Q15828-PRO_0000006648	uniprotkb:Q99538-PRO_0000026502	intact:EBI-29014783	intact:EBI-29020361	psi-mi:q15828-pro_0000006648(display_long)|uniprotkb:CST6(gene name)|psi-mi:CST6(display_short)|uniprotkb:Cystatin-E(gene name synonym)|uniprotkb:Cystatin-6(gene name synonym)	psi-mi:q99538-pro_0000026502(display_long)|uniprotkb:Asparaginyl endopeptidase(gene name synonym)|uniprotkb:Protease, cysteine 1(gene name synonym)|uniprotkb:LGMN(gene name)|psi-mi:LGMN(display_short)|uniprotkb:PRSC1(gene name synonym)	psi-mi:"MI:0415"(enzymatic study)	Dall et al. (2015)	pubmed:25630877|imex:IM-29408	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0414"(enzymatic reaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-28965837|imex:IM-29408-11	-	-	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	intact:EBI-7163527(chain-parent)	intact:EBI-2511794(chain-parent)	-	chain-seq-start:29|chain-seq-end:149	chain-seq-start:18|chain-seq-end:323	figure legend:Figs. 2C, 3, Supp. figure 4D,5D|comment:"Cys-172 of LGMN was oxidised by adding S-methyl methanethiosulfonate (MMTS), resulting in the mixed disulfide Cys172-S-CH3. At pH6 a cleaved CST6 peptide bond was religated by LGMN."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/11/29	2022/01/18	rogid:5J+QcWmPpP40Ntkt4Eu3bUYEO9k9606	rogid:MF5hIURQx43Y8UHYKcoAmFEBbJ09606	rigid:xZbvgkCfBEJMOO1vTs7UqM+MW/0	false	sufficient binding region:?-?|mutation disrupting interaction rate:36-36	mutation disrupting interaction rate:130-130|modified L-cysteine residue:172-172	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:P01034-PRO_0000006639	uniprotkb:Q99538-PRO_0000026502	intact:EBI-29036734	intact:EBI-29020361	psi-mi:p01034-pro_0000006639(display_long)|uniprotkb:Cystatin-3(gene name synonym)|uniprotkb:Gamma-trace(gene name synonym)|uniprotkb:Neuroendocrine basic polypeptide(gene name synonym)|uniprotkb:Post-gamma-globulin(gene name synonym)|uniprotkb:CST3(gene name)|psi-mi:CST3(display_short)	psi-mi:q99538-pro_0000026502(display_long)|uniprotkb:Asparaginyl endopeptidase(gene name synonym)|uniprotkb:Protease, cysteine 1(gene name synonym)|uniprotkb:LGMN(gene name)|psi-mi:LGMN(display_short)|uniprotkb:PRSC1(gene name synonym)	psi-mi:"MI:0415"(enzymatic study)	Dall et al. (2015)	pubmed:25630877|imex:IM-29408	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0414"(enzymatic reaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-28966029|imex:IM-29408-29	-	-	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	intact:EBI-948622(chain-parent)	intact:EBI-2511794(chain-parent)	-	chain-seq-start:27|chain-seq-end:146	chain-seq-start:18|chain-seq-end:323	figure legend:Supp. Fig. 4 A/B|comment:"Cys-172 of LGMN was oxidised by adding S-methyl methanethiosulfonate (MMTS), resulting in the mixed disulfide Cys172-S-CH3."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/11/29	2022/01/18	rogid:tkUZLEEhONKxhyOw2RooehXz0GI9606	rogid:MF5hIURQx43Y8UHYKcoAmFEBbJ09606	rigid:TyrEPQpMOH86K+gh4h3RukyEYlM	false	sufficient binding region:?-?	modified L-cysteine residue:172-172	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:Q99538-PRO_0000026502	-	intact:EBI-29020361	-	psi-mi:q99538-pro_0000026502(display_long)|uniprotkb:Asparaginyl endopeptidase(gene name synonym)|uniprotkb:Protease, cysteine 1(gene name synonym)|uniprotkb:LGMN(gene name)|psi-mi:LGMN(display_short)|uniprotkb:PRSC1(gene name synonym)	-	psi-mi:"MI:0415"(enzymatic study)	Dall et al. (2015)	pubmed:25630877|imex:IM-29408	taxid:9606(human)|taxid:9606(Homo sapiens)	-	psi-mi:"MI:0414"(enzymatic reaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-28966042|imex:IM-29408-31	-	-	psi-mi:"MI:0898"(putative self)	-	psi-mi:"MI:0898"(putative self)	-	psi-mi:"MI:0326"(protein)	-	intact:EBI-2511794(chain-parent)	-	-	chain-seq-start:18|chain-seq-end:323	-	figure legend:Supp. Fig. 4C|comment:"Cys-172 of LGMN was oxidised by adding S-methyl methanethiosulfonate (MMTS), resulting in the mixed disulfide Cys172-S-CH3."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/11/29	2022/01/18	rogid:MF5hIURQx43Y8UHYKcoAmFEBbJ09606	-	-	false	modified L-cysteine residue:172-172|mutation:292-292,286-286|sufficient binding region:?-?	-	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	-
uniprotkb:Q15828-PRO_0000006648	uniprotkb:Q99538-PRO_0000026502	intact:EBI-29014783	intact:EBI-29020361	psi-mi:q15828-pro_0000006648(display_long)|uniprotkb:CST6(gene name)|psi-mi:CST6(display_short)|uniprotkb:Cystatin-E(gene name synonym)|uniprotkb:Cystatin-6(gene name synonym)	psi-mi:q99538-pro_0000026502(display_long)|uniprotkb:Asparaginyl endopeptidase(gene name synonym)|uniprotkb:Protease, cysteine 1(gene name synonym)|uniprotkb:LGMN(gene name)|psi-mi:LGMN(display_short)|uniprotkb:PRSC1(gene name synonym)	psi-mi:"MI:1235"(thermal shift binding)	Dall et al. (2015)	pubmed:25630877|imex:IM-29408	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-28965943|imex:IM-29408-25	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	intact:EBI-7163527(chain-parent)	intact:EBI-2511794(chain-parent)	-	chain-seq-start:29|chain-seq-end:149	chain-seq-start:18|chain-seq-end:323	figure legend:Supp. Fig. 2B|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/11/29	2022/01/18	rogid:5J+QcWmPpP40Ntkt4Eu3bUYEO9k9606	rogid:MF5hIURQx43Y8UHYKcoAmFEBbJ09606	rigid:xZbvgkCfBEJMOO1vTs7UqM+MW/0	false	fusion protein:n-n|his tag:c-c	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P01034-PRO_0000006639	uniprotkb:Q99538-PRO_0000026502	intact:EBI-29036734	intact:EBI-29020361	psi-mi:p01034-pro_0000006639(display_long)|uniprotkb:Cystatin-3(gene name synonym)|uniprotkb:Gamma-trace(gene name synonym)|uniprotkb:Neuroendocrine basic polypeptide(gene name synonym)|uniprotkb:Post-gamma-globulin(gene name synonym)|uniprotkb:CST3(gene name)|psi-mi:CST3(display_short)	psi-mi:q99538-pro_0000026502(display_long)|uniprotkb:Asparaginyl endopeptidase(gene name synonym)|uniprotkb:Protease, cysteine 1(gene name synonym)|uniprotkb:LGMN(gene name)|psi-mi:LGMN(display_short)|uniprotkb:PRSC1(gene name synonym)	psi-mi:"MI:1235"(thermal shift binding)	Dall et al. (2015)	pubmed:25630877|imex:IM-29408	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-28965954|imex:IM-29408-27	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	intact:EBI-948622(chain-parent)	intact:EBI-2511794(chain-parent)	-	chain-seq-start:27|chain-seq-end:146	chain-seq-start:18|chain-seq-end:323	figure legend:Supp. Fig. 2B|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/11/29	2022/01/18	rogid:tkUZLEEhONKxhyOw2RooehXz0GI9606	rogid:MF5hIURQx43Y8UHYKcoAmFEBbJ09606	rigid:TyrEPQpMOH86K+gh4h3RukyEYlM	false	his tag:c-c|fusion protein:n-n	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)