#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q9BXR0	uniprotkb:Q9H974	intact:EBI-716832|uniprotkb:Q96BQ4|uniprotkb:Q9BXQ9|uniprotkb:B4DFM7|ensembl:ENSP00000250237	intact:EBI-1221028|uniprotkb:Q6IA59|uniprotkb:A6NGE9|uniprotkb:B7Z472|uniprotkb:B7Z5R2|uniprotkb:J3KR78|ensembl:ENSP00000281273	psi-mi:tgt_human(display_long)|uniprotkb:tRNA-guanine transglycosylase(gene name synonym)|uniprotkb:Guanine insertion enzyme(gene name synonym)|uniprotkb:QTRT1(gene name)|psi-mi:QTRT1(display_short)|uniprotkb:TGT(gene name synonym)|uniprotkb:TGUT(gene name synonym)	psi-mi:qtrt2_human(display_long)|uniprotkb:Queuine tRNA-ribosyltransferase domain-containing protein 1(gene name synonym)|uniprotkb:QTRT2(gene name)|psi-mi:QTRT2(display_short)|uniprotkb:QTRTD1(gene name synonym)	psi-mi:"MI:0091"(chromatography technology)	Chen et al. (2010)	pubmed:20354154|imex:IM-28909	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-26963009|imex:IM-28909-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_112486.1|ensembl:ENSG00000213339(gene)|ensembl:ENST00000250237(transcript)|go:"GO:0005634"(nucleus)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0006400"(tRNA modification)|go:"GO:0008479"(queuine tRNA-ribosyltransferase activity)|go:"GO:0032991"(protein-containing complex)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0101030"(tRNA-guanine transglycosylation)|interpro:IPR002616(Queuine/other tRNA-ribosyltransferase)|interpro:IPR004803(Queuine tRNA-ribosyltransferase)|interpro:IPR036511|rcsb pdb:6H42|rcsb pdb:6H45|reactome:R-HSA-6782315|go:"GO:0046872"(metal ion binding)	refseq:NP_078914.1|refseq:NP_001243764.1|refseq:NP_001243765.1|refseq:NP_001243766.1|refseq:XP_005247827.1|refseq:XP_016862671.1|refseq:XP_016862672.1|ensembl:ENSG00000151576(gene)|ensembl:ENST00000281273(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0008479"(queuine tRNA-ribosyltransferase activity)|go:"GO:0032991"(protein-containing complex)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0046872"(metal ion binding)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0101030"(tRNA-guanine transglycosylation)|interpro:IPR002616(Queuine/other tRNA-ribosyltransferase)|interpro:IPR028592|interpro:IPR036511|reactome:R-HSA-6782315	-	-	-	figure legend:Figs. 2,3|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2021/04/26	2021/04/26	rogid:0Z1Trg9nHJebKDjnOB6gBKvoMxA9606	rogid:8OQIoDPH8P/UdGe3VQO9/4KdpFs9606	rigid:EFfkdD6hfTBq2rRQraSz3cjxUA0	false	his tag:?-?	-	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:Q9BXR0	uniprotkb:Q9H974	intact:EBI-716832|uniprotkb:Q96BQ4|uniprotkb:Q9BXQ9|uniprotkb:B4DFM7|ensembl:ENSP00000250237	intact:EBI-1221028|uniprotkb:Q6IA59|uniprotkb:A6NGE9|uniprotkb:B7Z472|uniprotkb:B7Z5R2|uniprotkb:J3KR78|ensembl:ENSP00000281273	psi-mi:tgt_human(display_long)|uniprotkb:tRNA-guanine transglycosylase(gene name synonym)|uniprotkb:Guanine insertion enzyme(gene name synonym)|uniprotkb:QTRT1(gene name)|psi-mi:QTRT1(display_short)|uniprotkb:TGT(gene name synonym)|uniprotkb:TGUT(gene name synonym)	psi-mi:qtrt2_human(display_long)|uniprotkb:Queuine tRNA-ribosyltransferase domain-containing protein 1(gene name synonym)|uniprotkb:QTRT2(gene name)|psi-mi:QTRT2(display_short)|uniprotkb:QTRTD1(gene name synonym)	psi-mi:"MI:0030"(cross-linking study)	Chen et al. (2010)	pubmed:20354154|imex:IM-28909	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-26963022|imex:IM-28909-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_112486.1|ensembl:ENSG00000213339(gene)|ensembl:ENST00000250237(transcript)|go:"GO:0005634"(nucleus)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0006400"(tRNA modification)|go:"GO:0008479"(queuine tRNA-ribosyltransferase activity)|go:"GO:0032991"(protein-containing complex)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0101030"(tRNA-guanine transglycosylation)|interpro:IPR002616(Queuine/other tRNA-ribosyltransferase)|interpro:IPR004803(Queuine tRNA-ribosyltransferase)|interpro:IPR036511|rcsb pdb:6H42|rcsb pdb:6H45|reactome:R-HSA-6782315|go:"GO:0046872"(metal ion binding)	refseq:NP_078914.1|refseq:NP_001243764.1|refseq:NP_001243765.1|refseq:NP_001243766.1|refseq:XP_005247827.1|refseq:XP_016862671.1|refseq:XP_016862672.1|ensembl:ENSG00000151576(gene)|ensembl:ENST00000281273(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0008479"(queuine tRNA-ribosyltransferase activity)|go:"GO:0032991"(protein-containing complex)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0046872"(metal ion binding)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0101030"(tRNA-guanine transglycosylation)|interpro:IPR002616(Queuine/other tRNA-ribosyltransferase)|interpro:IPR028592|interpro:IPR036511|reactome:R-HSA-6782315	-	-	-	figure legend:Fig. 4, Supp fig. 3, Supp Table 3|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/04/26	2021/04/26	rogid:0Z1Trg9nHJebKDjnOB6gBKvoMxA9606	rogid:8OQIoDPH8P/UdGe3VQO9/4KdpFs9606	rigid:EFfkdD6hfTBq2rRQraSz3cjxUA0	false	his tag:?-?	-	1	1	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
chebi:"CHEBI:16235"	rhea:"RHEA:16633"	intact:EBI-26963675|chebi:"CHEBI:42948"|chebi:"CHEBI:14371"|chebi:"CHEBI:14372"|chebi:"CHEBI:24443"|chebi:"CHEBI:5563"	intact:EBI-26963029|intenz:2.4.2.64|reactome:R-HSA-6782406	psi-mi:guanine(display_short)|psi-mi:"CHEBI:16235"(display_long)|intact:2-Amino-6-hydroxypurine(synonym)|intact:2-amino-6-oxopurine(synonym)|intact:G(synonym)|intact:Gua(synonym)|intact:Guanine(synonym)|intact:GUANINE(synonym)|intact:guanine(synonym)|intact:2-amino-1,9-dihydro-6H-purin-6-one(iupac name)	psi-mi:tgt_human-1(display_short)|psi-mi:"RHEA:16633"(display_long)|intact:Queuine tRNA-ribosyltransferase complex(complex synonym)|intact:QTRT1-QTRTD1 transglycosylase complex(complex synonym)|intact:tRNA-guanine transglycosylase complex(complex recommended name)|intact:"QTRT1:QTRT2"(complex systematic name)|intact:TGT complex(complex synonym)	psi-mi:"MI:0415"(enzymatic study)	Chen et al. (2010)	pubmed:20354154|imex:IM-28909	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0414"(enzymatic reaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-26963500|imex:IM-28909-5	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	go:"GO:1990234"(transferase complex)|pubmed:33658722(see-also)|pubmed:20354154(see-also)|go:"GO:0101030"(tRNA-guanine transglycosylation)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0008479"(queuine tRNA-ribosyltransferase activity)|intact:EBI-26963022(exp-evidence)|complex portal:CPX-6662(complex-primary)|evidence ontology:"ECO:0000353"	-	-	curated-complex:"Catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Q34 appears to enhance the speed of translation of C-ending codons but reduced that of U-ending codons. In human cytoplasm, tRNAs for His and Asn have queuosine, whilst in mitochondria, queuosine occurs at the wobble positions of tRNAs for Tyr, His, Asn and Asp."|complex-assembly:Heterodimer	figure legend:Figs. 5, 6, Table 1|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/04/26	2021/04/27	-	-	-	false	14c radiolabel:?-?	his tag:?-?|mutation disrupting interaction strength:279-279	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:16235"	chebi:"CHEBI:29182"	intact:EBI-26963675|chebi:"CHEBI:42948"|chebi:"CHEBI:14371"|chebi:"CHEBI:14372"|chebi:"CHEBI:24443"|chebi:"CHEBI:5563"	intact:EBI-26963493|chebi:"CHEBI:15189"|chebi:"CHEBI:10692"	psi-mi:guanine(display_short)|psi-mi:"CHEBI:16235"(display_long)|intact:2-Amino-6-hydroxypurine(synonym)|intact:2-amino-6-oxopurine(synonym)|intact:G(synonym)|intact:Gua(synonym)|intact:Guanine(synonym)|intact:GUANINE(synonym)|intact:guanine(synonym)|intact:2-amino-1,9-dihydro-6H-purin-6-one(iupac name)	psi-mi:"trna(tyr)"(display_short)|psi-mi:"CHEBI:29182"(display_long)|intact:transfer RNA-Tyr(synonym)|intact:"tRNA(Tyr)"(synonym)|intact:tyrosine tRNA(synonym)	psi-mi:"MI:0415"(enzymatic study)	Chen et al. (2010)	pubmed:20354154|imex:IM-28909	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	psi-mi:"MI:0414"(enzymatic reaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-26963500|imex:IM-28909-5	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0328"(small molecule)	-	-	-	-	-	figure legend:Figs. 5, 6, Table 1|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/04/26	2021/04/27	-	-	-	false	14c radiolabel:?-?	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)