#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:P40970 uniprotkb:P25045 intact:EBI-10067|uniprotkb:Q66RG8|uniprotkb:D6VS48|ensemblfungi:YDR062W intact:EBI-10059|uniprotkb:D6W0C3|ensemblfungi:YMR296C psi-mi:lcb2_yeast(display_long)|uniprotkb:LCB2(gene name)|psi-mi:LCB2(display_short)|uniprotkb:SCS1(gene name synonym)|uniprotkb:YDR062W(locus name)|uniprotkb:YD9609.16(orf name)|uniprotkb:D4246(orf name)|uniprotkb:TSC1(gene name synonym)|uniprotkb:Long chain base biosynthesis protein 2(gene name synonym) psi-mi:lcb1_yeast(display_long)|uniprotkb:YMR296C(locus name)|uniprotkb:LCB1(gene name)|psi-mi:LCB1(display_short)|uniprotkb:END8(gene name synonym)|uniprotkb:TSC2(gene name synonym)|uniprotkb:Long chain base biosynthesis protein 1(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Gable et al. (2000) pubmed:10713067|imex:IM-20228 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-2206671|imex:IM-20228-1 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_010347.1|sgd:S000002469|ensemblfungi:YDR062W_mRNA(transcript)|go:"GO:0004758"(serine C-palmitoyltransferase activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0016021"(integral component of membrane)|go:"GO:0017059"(serine C-palmitoyltransferase complex)|go:"GO:0030148"(sphingolipid biosynthetic process)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0035339"(SPOTS complex)|go:"GO:0046512"(sphingosine biosynthetic process)|go:"GO:0046513"(ceramide biosynthetic process)|interpro:IPR001917(Aminotransferase, class-II, pyridoxal-phosphate binding site)|interpro:IPR004839(Aminotransferase, class I and II)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|ensemblfungi:YDR062W(gene)|dip:DIP-6658N refseq:NP_014025.1|sgd:S000004911|ensemblfungi:YMR296C_mRNA(transcript)|go:"GO:0004758"(serine C-palmitoyltransferase activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0016021"(integral component of membrane)|go:"GO:0017059"(serine C-palmitoyltransferase complex)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0035339"(SPOTS complex)|go:"GO:0046512"(sphingosine biosynthetic process)|go:"GO:0046513"(ceramide biosynthetic process)|interpro:IPR004839(Aminotransferase, class I and II)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|ensemblfungi:YMR296C(gene)|dip:DIP-5249N|mint:P25045 - crc64:06DF4A709CC9F29B crc64:9F7F93E4B2C70FDB figure legend:Figs. 5A and 5B|comment:Interaction does not require the presence of Tsc3|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2009/01/15 2014/10/16 rogid:9HWPnbYvkOQbNXB583l8v3dkiz8559292 rogid:maw7eX1MYPojWGQUtWBPX7Qn8wI559292 intact-crc:800C2E5F24E086BD|rigid:72JxauQn1o9xErNaz32tGoI6I00 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P40970 uniprotkb:P25045 intact:EBI-10067|uniprotkb:Q66RG8|uniprotkb:D6VS48|ensemblfungi:YDR062W intact:EBI-10059|uniprotkb:D6W0C3|ensemblfungi:YMR296C psi-mi:lcb2_yeast(display_long)|uniprotkb:LCB2(gene name)|psi-mi:LCB2(display_short)|uniprotkb:SCS1(gene name synonym)|uniprotkb:YDR062W(locus name)|uniprotkb:YD9609.16(orf name)|uniprotkb:D4246(orf name)|uniprotkb:TSC1(gene name synonym)|uniprotkb:Long chain base biosynthesis protein 2(gene name synonym) psi-mi:lcb1_yeast(display_long)|uniprotkb:YMR296C(locus name)|uniprotkb:LCB1(gene name)|psi-mi:LCB1(display_short)|uniprotkb:END8(gene name synonym)|uniprotkb:TSC2(gene name synonym)|uniprotkb:Long chain base biosynthesis protein 1(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Gable et al. (2000) pubmed:10713067|imex:IM-20228 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-2206676|imex:IM-20228-2 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_010347.1|sgd:S000002469|ensemblfungi:YDR062W_mRNA(transcript)|go:"GO:0004758"(serine C-palmitoyltransferase activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0016021"(integral component of membrane)|go:"GO:0017059"(serine C-palmitoyltransferase complex)|go:"GO:0030148"(sphingolipid biosynthetic process)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0035339"(SPOTS complex)|go:"GO:0046512"(sphingosine biosynthetic process)|go:"GO:0046513"(ceramide biosynthetic process)|interpro:IPR001917(Aminotransferase, class-II, pyridoxal-phosphate binding site)|interpro:IPR004839(Aminotransferase, class I and II)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|ensemblfungi:YDR062W(gene)|dip:DIP-6658N refseq:NP_014025.1|sgd:S000004911|ensemblfungi:YMR296C_mRNA(transcript)|go:"GO:0004758"(serine C-palmitoyltransferase activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0016021"(integral component of membrane)|go:"GO:0017059"(serine C-palmitoyltransferase complex)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0035339"(SPOTS complex)|go:"GO:0046512"(sphingosine biosynthetic process)|go:"GO:0046513"(ceramide biosynthetic process)|interpro:IPR004839(Aminotransferase, class I and II)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|ensemblfungi:YMR296C(gene)|dip:DIP-5249N|mint:P25045 - crc64:06DF4A709CC9F29B crc64:9F7F93E4B2C70FDB figure legend:Figs. 5A and 5B|comment:Interaction does not require the presence of Tsc3|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2009/01/15 2014/10/16 rogid:9HWPnbYvkOQbNXB583l8v3dkiz8559292 rogid:maw7eX1MYPojWGQUtWBPX7Qn8wI559292 intact-crc:58C6C8D4FF331A7B|rigid:72JxauQn1o9xErNaz32tGoI6I00 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P25045 uniprotkb:P40970 intact:EBI-10059|uniprotkb:D6W0C3|ensemblfungi:YMR296C intact:EBI-10067|uniprotkb:Q66RG8|uniprotkb:D6VS48|ensemblfungi:YDR062W psi-mi:lcb1_yeast(display_long)|uniprotkb:YMR296C(locus name)|uniprotkb:LCB1(gene name)|psi-mi:LCB1(display_short)|uniprotkb:END8(gene name synonym)|uniprotkb:TSC2(gene name synonym)|uniprotkb:Long chain base biosynthesis protein 1(gene name synonym) psi-mi:lcb2_yeast(display_long)|uniprotkb:LCB2(gene name)|psi-mi:LCB2(display_short)|uniprotkb:SCS1(gene name synonym)|uniprotkb:YDR062W(locus name)|uniprotkb:YD9609.16(orf name)|uniprotkb:D4246(orf name)|uniprotkb:TSC1(gene name synonym)|uniprotkb:Long chain base biosynthesis protein 2(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Gable et al. (2000) pubmed:10713067|imex:IM-20228 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-2206681|imex:IM-20228-3 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_014025.1|sgd:S000004911|ensemblfungi:YMR296C_mRNA(transcript)|go:"GO:0004758"(serine C-palmitoyltransferase activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0016021"(integral component of membrane)|go:"GO:0017059"(serine C-palmitoyltransferase complex)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0035339"(SPOTS complex)|go:"GO:0046512"(sphingosine biosynthetic process)|go:"GO:0046513"(ceramide biosynthetic process)|interpro:IPR004839(Aminotransferase, class I and II)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|ensemblfungi:YMR296C(gene)|dip:DIP-5249N|mint:P25045 refseq:NP_010347.1|sgd:S000002469|ensemblfungi:YDR062W_mRNA(transcript)|go:"GO:0004758"(serine C-palmitoyltransferase activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0016021"(integral component of membrane)|go:"GO:0017059"(serine C-palmitoyltransferase complex)|go:"GO:0030148"(sphingolipid biosynthetic process)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0035339"(SPOTS complex)|go:"GO:0046512"(sphingosine biosynthetic process)|go:"GO:0046513"(ceramide biosynthetic process)|interpro:IPR001917(Aminotransferase, class-II, pyridoxal-phosphate binding site)|interpro:IPR004839(Aminotransferase, class I and II)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|ensemblfungi:YDR062W(gene)|dip:DIP-6658N - crc64:9F7F93E4B2C70FDB crc64:06DF4A709CC9F29B figure legend:Fig. 5A|comment:Tagged protein was expressed in cells lacking lcb1|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2009/01/15 2014/10/16 rogid:maw7eX1MYPojWGQUtWBPX7Qn8wI559292 rogid:9HWPnbYvkOQbNXB583l8v3dkiz8559292 intact-crc:3903D5A81E739DE2|rigid:72JxauQn1o9xErNaz32tGoI6I00 false glutathione s tranferase tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P40970 uniprotkb:P25045 intact:EBI-10067|uniprotkb:Q66RG8|uniprotkb:D6VS48|ensemblfungi:YDR062W intact:EBI-10059|uniprotkb:D6W0C3|ensemblfungi:YMR296C psi-mi:lcb2_yeast(display_long)|uniprotkb:LCB2(gene name)|psi-mi:LCB2(display_short)|uniprotkb:SCS1(gene name synonym)|uniprotkb:YDR062W(locus name)|uniprotkb:YD9609.16(orf name)|uniprotkb:D4246(orf name)|uniprotkb:TSC1(gene name synonym)|uniprotkb:Long chain base biosynthesis protein 2(gene name synonym) psi-mi:lcb1_yeast(display_long)|uniprotkb:YMR296C(locus name)|uniprotkb:LCB1(gene name)|psi-mi:LCB1(display_short)|uniprotkb:END8(gene name synonym)|uniprotkb:TSC2(gene name synonym)|uniprotkb:Long chain base biosynthesis protein 1(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Gable et al. (2000) pubmed:10713067|imex:IM-20228 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-2206689|imex:IM-20228-4 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_010347.1|sgd:S000002469|ensemblfungi:YDR062W_mRNA(transcript)|go:"GO:0004758"(serine C-palmitoyltransferase activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0016021"(integral component of membrane)|go:"GO:0017059"(serine C-palmitoyltransferase complex)|go:"GO:0030148"(sphingolipid biosynthetic process)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0035339"(SPOTS complex)|go:"GO:0046512"(sphingosine biosynthetic process)|go:"GO:0046513"(ceramide biosynthetic process)|interpro:IPR001917(Aminotransferase, class-II, pyridoxal-phosphate binding site)|interpro:IPR004839(Aminotransferase, class I and II)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|ensemblfungi:YDR062W(gene)|dip:DIP-6658N refseq:NP_014025.1|sgd:S000004911|ensemblfungi:YMR296C_mRNA(transcript)|go:"GO:0004758"(serine C-palmitoyltransferase activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0016021"(integral component of membrane)|go:"GO:0017059"(serine C-palmitoyltransferase complex)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0035339"(SPOTS complex)|go:"GO:0046512"(sphingosine biosynthetic process)|go:"GO:0046513"(ceramide biosynthetic process)|interpro:IPR004839(Aminotransferase, class I and II)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|ensemblfungi:YMR296C(gene)|dip:DIP-5249N|mint:P25045 - crc64:06DF4A709CC9F29B crc64:9F7F93E4B2C70FDB figure legend:Fig. 5A|comment:Tagged protein was expressed in cells lacking lcb1|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2009/01/15 2014/10/16 rogid:9HWPnbYvkOQbNXB583l8v3dkiz8559292 rogid:maw7eX1MYPojWGQUtWBPX7Qn8wI559292 intact-crc:5970AAEC4F4889E8|rigid:72JxauQn1o9xErNaz32tGoI6I00 false - glutathione s tranferase tag:?-? - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P40970 uniprotkb:Q3E790 intact:EBI-10067|uniprotkb:Q66RG8|uniprotkb:D6VS48|ensemblfungi:YDR062W intact:EBI-2097198|uniprotkb:D6VQ58|ensemblfungi:YBR058C-A psi-mi:lcb2_yeast(display_long)|uniprotkb:LCB2(gene name)|psi-mi:LCB2(display_short)|uniprotkb:SCS1(gene name synonym)|uniprotkb:YDR062W(locus name)|uniprotkb:YD9609.16(orf name)|uniprotkb:D4246(orf name)|uniprotkb:TSC1(gene name synonym)|uniprotkb:Long chain base biosynthesis protein 2(gene name synonym) psi-mi:tsc3_yeast(display_long)|uniprotkb:TSC3(gene name)|psi-mi:TSC3(display_short)|uniprotkb:Temperature-sensitive CSG2-mutant suppressor protein 3(gene name synonym)|uniprotkb:YBR058C-A(locus name) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Gable et al. (2000) pubmed:10713067|imex:IM-20228 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-2206772|imex:IM-20228-5 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_010347.1|sgd:S000002469|ensemblfungi:YDR062W_mRNA(transcript)|go:"GO:0004758"(serine C-palmitoyltransferase activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0016021"(integral component of membrane)|go:"GO:0017059"(serine C-palmitoyltransferase complex)|go:"GO:0030148"(sphingolipid biosynthetic process)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0035339"(SPOTS complex)|go:"GO:0046512"(sphingosine biosynthetic process)|go:"GO:0046513"(ceramide biosynthetic process)|interpro:IPR001917(Aminotransferase, class-II, pyridoxal-phosphate binding site)|interpro:IPR004839(Aminotransferase, class I and II)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|ensemblfungi:YDR062W(gene)|dip:DIP-6658N sgd:S000007521|refseq:NP_116327.3|dip:DIP-47347N|ensemblfungi:YBR058C-A(gene)|ensemblfungi:YBR058C-A_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0006666"(3-keto-sphinganine metabolic process)|go:"GO:0008047"(enzyme activator activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0035339"(SPOTS complex)|go:"GO:0090154"(positive regulation of sphingolipid biosynthetic process) - crc64:06DF4A709CC9F29B crc64:F23EE19603FEC87E figure legend:Fig. 6C|comment:Tsc3 is lost from this protein complex at high salt concentrations|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2009/01/15 2014/10/16 rogid:9HWPnbYvkOQbNXB583l8v3dkiz8559292 rogid:RS7OZG+yOS2PFbpRdmYX2g8sjuw559292 intact-crc:30D0F242173305FF|rigid:YeqCenOKv2DCLaVMbBItJ2r3SEI false - ha tag:?-? - - psi-mi:"MI:0705"(anti tag western blot) psi-mi:"MI:0705"(anti tag western blot) uniprotkb:P40970 uniprotkb:P25045 intact:EBI-10067|uniprotkb:Q66RG8|uniprotkb:D6VS48|ensemblfungi:YDR062W intact:EBI-10059|uniprotkb:D6W0C3|ensemblfungi:YMR296C psi-mi:lcb2_yeast(display_long)|uniprotkb:LCB2(gene name)|psi-mi:LCB2(display_short)|uniprotkb:SCS1(gene name synonym)|uniprotkb:YDR062W(locus name)|uniprotkb:YD9609.16(orf name)|uniprotkb:D4246(orf name)|uniprotkb:TSC1(gene name synonym)|uniprotkb:Long chain base biosynthesis protein 2(gene name synonym) psi-mi:lcb1_yeast(display_long)|uniprotkb:YMR296C(locus name)|uniprotkb:LCB1(gene name)|psi-mi:LCB1(display_short)|uniprotkb:END8(gene name synonym)|uniprotkb:TSC2(gene name synonym)|uniprotkb:Long chain base biosynthesis protein 1(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Gable et al. (2000) pubmed:10713067|imex:IM-20228 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-2206772|imex:IM-20228-5 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_010347.1|sgd:S000002469|ensemblfungi:YDR062W_mRNA(transcript)|go:"GO:0004758"(serine C-palmitoyltransferase activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0016021"(integral component of membrane)|go:"GO:0017059"(serine C-palmitoyltransferase complex)|go:"GO:0030148"(sphingolipid biosynthetic process)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0035339"(SPOTS complex)|go:"GO:0046512"(sphingosine biosynthetic process)|go:"GO:0046513"(ceramide biosynthetic process)|interpro:IPR001917(Aminotransferase, class-II, pyridoxal-phosphate binding site)|interpro:IPR004839(Aminotransferase, class I and II)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|ensemblfungi:YDR062W(gene)|dip:DIP-6658N refseq:NP_014025.1|sgd:S000004911|ensemblfungi:YMR296C_mRNA(transcript)|go:"GO:0004758"(serine C-palmitoyltransferase activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0016021"(integral component of membrane)|go:"GO:0017059"(serine C-palmitoyltransferase complex)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0035339"(SPOTS complex)|go:"GO:0046512"(sphingosine biosynthetic process)|go:"GO:0046513"(ceramide biosynthetic process)|interpro:IPR004839(Aminotransferase, class I and II)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|ensemblfungi:YMR296C(gene)|dip:DIP-5249N|mint:P25045 - crc64:06DF4A709CC9F29B crc64:9F7F93E4B2C70FDB figure legend:Fig. 6C|comment:Tsc3 is lost from this protein complex at high salt concentrations|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2009/01/15 2014/10/16 rogid:9HWPnbYvkOQbNXB583l8v3dkiz8559292 rogid:maw7eX1MYPojWGQUtWBPX7Qn8wI559292 intact-crc:30D0F242173305FF|rigid:YeqCenOKv2DCLaVMbBItJ2r3SEI false - - - - psi-mi:"MI:0705"(anti tag western blot) psi-mi:"MI:0705"(anti tag western blot) uniprotkb:P25045 uniprotkb:Q3E790 intact:EBI-10059|uniprotkb:D6W0C3|ensemblfungi:YMR296C intact:EBI-2097198|uniprotkb:D6VQ58|ensemblfungi:YBR058C-A psi-mi:lcb1_yeast(display_long)|uniprotkb:YMR296C(locus name)|uniprotkb:LCB1(gene name)|psi-mi:LCB1(display_short)|uniprotkb:END8(gene name synonym)|uniprotkb:TSC2(gene name synonym)|uniprotkb:Long chain base biosynthesis protein 1(gene name synonym) psi-mi:tsc3_yeast(display_long)|uniprotkb:TSC3(gene name)|psi-mi:TSC3(display_short)|uniprotkb:Temperature-sensitive CSG2-mutant suppressor protein 3(gene name synonym)|uniprotkb:YBR058C-A(locus name) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Gable et al. (2000) pubmed:10713067|imex:IM-20228 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-2206778|imex:IM-20228-6 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_014025.1|sgd:S000004911|ensemblfungi:YMR296C_mRNA(transcript)|go:"GO:0004758"(serine C-palmitoyltransferase activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0016021"(integral component of membrane)|go:"GO:0017059"(serine C-palmitoyltransferase complex)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0035339"(SPOTS complex)|go:"GO:0046512"(sphingosine biosynthetic process)|go:"GO:0046513"(ceramide biosynthetic process)|interpro:IPR004839(Aminotransferase, class I and II)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|ensemblfungi:YMR296C(gene)|dip:DIP-5249N|mint:P25045 sgd:S000007521|refseq:NP_116327.3|dip:DIP-47347N|ensemblfungi:YBR058C-A(gene)|ensemblfungi:YBR058C-A_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0006666"(3-keto-sphinganine metabolic process)|go:"GO:0008047"(enzyme activator activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0035339"(SPOTS complex)|go:"GO:0090154"(positive regulation of sphingolipid biosynthetic process) - crc64:9F7F93E4B2C70FDB crc64:F23EE19603FEC87E figure legend:Fig. 6C|comment:Tsc3 is lost from this protein complex at high salt concentrations|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2009/01/15 2014/10/16 rogid:maw7eX1MYPojWGQUtWBPX7Qn8wI559292 rogid:RS7OZG+yOS2PFbpRdmYX2g8sjuw559292 intact-crc:6CC9DE0827920CDD|rigid:YeqCenOKv2DCLaVMbBItJ2r3SEI false - ha tag:?-? - - psi-mi:"MI:0705"(anti tag western blot) psi-mi:"MI:0705"(anti tag western blot) uniprotkb:P25045 uniprotkb:P40970 intact:EBI-10059|uniprotkb:D6W0C3|ensemblfungi:YMR296C intact:EBI-10067|uniprotkb:Q66RG8|uniprotkb:D6VS48|ensemblfungi:YDR062W psi-mi:lcb1_yeast(display_long)|uniprotkb:YMR296C(locus name)|uniprotkb:LCB1(gene name)|psi-mi:LCB1(display_short)|uniprotkb:END8(gene name synonym)|uniprotkb:TSC2(gene name synonym)|uniprotkb:Long chain base biosynthesis protein 1(gene name synonym) psi-mi:lcb2_yeast(display_long)|uniprotkb:LCB2(gene name)|psi-mi:LCB2(display_short)|uniprotkb:SCS1(gene name synonym)|uniprotkb:YDR062W(locus name)|uniprotkb:YD9609.16(orf name)|uniprotkb:D4246(orf name)|uniprotkb:TSC1(gene name synonym)|uniprotkb:Long chain base biosynthesis protein 2(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Gable et al. (2000) pubmed:10713067|imex:IM-20228 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-2206778|imex:IM-20228-6 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_014025.1|sgd:S000004911|ensemblfungi:YMR296C_mRNA(transcript)|go:"GO:0004758"(serine C-palmitoyltransferase activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0016021"(integral component of membrane)|go:"GO:0017059"(serine C-palmitoyltransferase complex)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0035339"(SPOTS complex)|go:"GO:0046512"(sphingosine biosynthetic process)|go:"GO:0046513"(ceramide biosynthetic process)|interpro:IPR004839(Aminotransferase, class I and II)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|ensemblfungi:YMR296C(gene)|dip:DIP-5249N|mint:P25045 refseq:NP_010347.1|sgd:S000002469|ensemblfungi:YDR062W_mRNA(transcript)|go:"GO:0004758"(serine C-palmitoyltransferase activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0016021"(integral component of membrane)|go:"GO:0017059"(serine C-palmitoyltransferase complex)|go:"GO:0030148"(sphingolipid biosynthetic process)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0035339"(SPOTS complex)|go:"GO:0046512"(sphingosine biosynthetic process)|go:"GO:0046513"(ceramide biosynthetic process)|interpro:IPR001917(Aminotransferase, class-II, pyridoxal-phosphate binding site)|interpro:IPR004839(Aminotransferase, class I and II)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|ensemblfungi:YDR062W(gene)|dip:DIP-6658N - crc64:9F7F93E4B2C70FDB crc64:06DF4A709CC9F29B figure legend:Fig. 6C|comment:Tsc3 is lost from this protein complex at high salt concentrations|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2009/01/15 2014/10/16 rogid:maw7eX1MYPojWGQUtWBPX7Qn8wI559292 rogid:9HWPnbYvkOQbNXB583l8v3dkiz8559292 intact-crc:6CC9DE0827920CDD|rigid:YeqCenOKv2DCLaVMbBItJ2r3SEI false - - - - psi-mi:"MI:0705"(anti tag western blot) psi-mi:"MI:0705"(anti tag western blot) uniprotkb:Q3E790 uniprotkb:P40970 intact:EBI-2097198|uniprotkb:D6VQ58|ensemblfungi:YBR058C-A intact:EBI-10067|uniprotkb:Q66RG8|uniprotkb:D6VS48|ensemblfungi:YDR062W psi-mi:tsc3_yeast(display_long)|uniprotkb:TSC3(gene name)|psi-mi:TSC3(display_short)|uniprotkb:Temperature-sensitive CSG2-mutant suppressor protein 3(gene name synonym)|uniprotkb:YBR058C-A(locus name) psi-mi:lcb2_yeast(display_long)|uniprotkb:LCB2(gene name)|psi-mi:LCB2(display_short)|uniprotkb:SCS1(gene name synonym)|uniprotkb:YDR062W(locus name)|uniprotkb:YD9609.16(orf name)|uniprotkb:D4246(orf name)|uniprotkb:TSC1(gene name synonym)|uniprotkb:Long chain base biosynthesis protein 2(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Gable et al. (2000) pubmed:10713067|imex:IM-20228 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-2206796|imex:IM-20228-7 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) sgd:S000007521|refseq:NP_116327.3|dip:DIP-47347N|ensemblfungi:YBR058C-A(gene)|ensemblfungi:YBR058C-A_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0006666"(3-keto-sphinganine metabolic process)|go:"GO:0008047"(enzyme activator activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0035339"(SPOTS complex)|go:"GO:0090154"(positive regulation of sphingolipid biosynthetic process) refseq:NP_010347.1|sgd:S000002469|ensemblfungi:YDR062W_mRNA(transcript)|go:"GO:0004758"(serine C-palmitoyltransferase activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0016021"(integral component of membrane)|go:"GO:0017059"(serine C-palmitoyltransferase complex)|go:"GO:0030148"(sphingolipid biosynthetic process)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0035339"(SPOTS complex)|go:"GO:0046512"(sphingosine biosynthetic process)|go:"GO:0046513"(ceramide biosynthetic process)|interpro:IPR001917(Aminotransferase, class-II, pyridoxal-phosphate binding site)|interpro:IPR004839(Aminotransferase, class I and II)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|ensemblfungi:YDR062W(gene)|dip:DIP-6658N - crc64:F23EE19603FEC87E crc64:06DF4A709CC9F29B figure legend:Fig. 6D|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2009/01/15 2014/10/16 rogid:RS7OZG+yOS2PFbpRdmYX2g8sjuw559292 rogid:9HWPnbYvkOQbNXB583l8v3dkiz8559292 intact-crc:CAA35773B1A28E35|rigid:YeqCenOKv2DCLaVMbBItJ2r3SEI false ha tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q3E790 uniprotkb:P25045 intact:EBI-2097198|uniprotkb:D6VQ58|ensemblfungi:YBR058C-A intact:EBI-10059|uniprotkb:D6W0C3|ensemblfungi:YMR296C psi-mi:tsc3_yeast(display_long)|uniprotkb:TSC3(gene name)|psi-mi:TSC3(display_short)|uniprotkb:Temperature-sensitive CSG2-mutant suppressor protein 3(gene name synonym)|uniprotkb:YBR058C-A(locus name) psi-mi:lcb1_yeast(display_long)|uniprotkb:YMR296C(locus name)|uniprotkb:LCB1(gene name)|psi-mi:LCB1(display_short)|uniprotkb:END8(gene name synonym)|uniprotkb:TSC2(gene name synonym)|uniprotkb:Long chain base biosynthesis protein 1(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Gable et al. (2000) pubmed:10713067|imex:IM-20228 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-2206796|imex:IM-20228-7 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) sgd:S000007521|refseq:NP_116327.3|dip:DIP-47347N|ensemblfungi:YBR058C-A(gene)|ensemblfungi:YBR058C-A_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0006666"(3-keto-sphinganine metabolic process)|go:"GO:0008047"(enzyme activator activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0035339"(SPOTS complex)|go:"GO:0090154"(positive regulation of sphingolipid biosynthetic process) refseq:NP_014025.1|sgd:S000004911|ensemblfungi:YMR296C_mRNA(transcript)|go:"GO:0004758"(serine C-palmitoyltransferase activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0016021"(integral component of membrane)|go:"GO:0017059"(serine C-palmitoyltransferase complex)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0035339"(SPOTS complex)|go:"GO:0046512"(sphingosine biosynthetic process)|go:"GO:0046513"(ceramide biosynthetic process)|interpro:IPR004839(Aminotransferase, class I and II)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|ensemblfungi:YMR296C(gene)|dip:DIP-5249N|mint:P25045 - crc64:F23EE19603FEC87E crc64:9F7F93E4B2C70FDB figure legend:Fig. 6D|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2009/01/15 2014/10/16 rogid:RS7OZG+yOS2PFbpRdmYX2g8sjuw559292 rogid:maw7eX1MYPojWGQUtWBPX7Qn8wI559292 intact-crc:CAA35773B1A28E35|rigid:YeqCenOKv2DCLaVMbBItJ2r3SEI false ha tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot)