IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ HY5 stability and activity in arabidopsis is regulated by phosphorylation in its COP1 binding domain. HY5 stability and activity in arabidopsis is regulated by phosphorylation in its COP1 binding domain. EMBO J. (0261-4189) 2000 imex curation Hardtke CS., Gohda K., Osterlund MT., Oyama T., Okada K., Deng XW. xingwang.deng@yale.edu 10-Jul-2009: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Hardtke CS., Gohda K., Osterlund MT., Oyama T., Okada K., Deng XW. It was not possible to determine the species of origin of the RBCS1a promoter , therefore interactions involving this promoter were not curated. details of the CHS1 promoter were taken from a reference provided in the text. xingwang.deng@yale.edu Accepted 2009-JUN-11 by ORCHARD. 10-Jul-2009: Contacted by JYOTI. EMBO J. (0261-4189) 2000 orchard Only protein-protein interactions imex curation Molecular interaction between COP1 and HY5 defines a regulatory switch for light control of Arabidopsis development. Molecular interaction between COP1 and HY5 defines a regulatory switch for light control of Arabidopsis development. Mol. Cell (1097-2765) 1998 imex curation Ang LH., Chattopadhyay S., Wei N., Oyama T., Okada K., Batschauer A., Deng XW. xingwang.deng@yale.edu 14-May-2008: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Ang LH., Chattopadhyay S., Wei N., Oyama T., Okada K., Batschauer A., Deng XW. xingwang.deng@yale.edu Accepted 2008-APR-28 by CDEROW. 14-May-2008: Contacted by JYOTI. Mol. Cell (1097-2765) 1998 Only protein-protein interactions imex curation chs-sinal-dna Chalcone synthase promoter Sinapis alba ds dna double stranded deoxyribonucleic acid ds DNA ds dna sinal Sinapis alba (White mustard) Brassica hirta White mustard The authors in the relevant paper (see PMID:9659918) describe this as "180 bp fragment of the CHS promoter that includes the Unit 1 region: 2247 to 268". They reference the promoter as the CHS promoter region from white mustard (Sinapis alba) and reference this with PMID 7599309. hy5_arath Transcription factor HY5 HY5 BZIP56 At5g11260 F2I11_150 Protein LONG HYPOCOTYL 5 bZIP transcription factor 56 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQEQATSSLAASSLPSSSERSSSSAPHLEIKEGIESDEEIRRVPEFGGEAVGKETSGRESGSATGQERTQATVGESQRKRGRTPAEKENKRLKRLLRNRVSAQQARERKKAYLSELENRVKDLENKNSELEERLSTLQNENQMLRHILKNTTGNKRGGGGGSNADASL 1E3C4672B192D8A9 hy5-dna 2 4 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag mutation analysis mutation analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) 3 unspecified role unspecified role bait bait direct interaction direct interaction Figure 6 HY5 Binds Specifically to the Light-Responsive Unit 1 Region (U1) of a CHS1 Promoter Binding of HY5 to the DNA probe was abolished by the presence of unlabelled synthetic consensus G-box tetramer and the DNA region described by the authors as Unit1 which contains the G box like motif. The DNA probe was 32P labelled. hy5-dna-1 1 4 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role bait bait direct interaction direct interaction Figure 4B We observed that for both the CHS1 and the RBCS1a minimal light inducible promoter fragments, dephosphorylation enhanced binding of HY5 to its DNA target in vitro ∼3- and 5-fold (as determined from scintillation counting of the retarded bands), respectively (Figure 4B and C). This result indicates that unphosphorylated HY5 is more effective in binding to these promoters. The authors concluded " unphosphorylated HY5 is more effective in binding to these promoters". MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Phylogenetic and functional features of the plastid transcription kinase cpCK2 from Arabidopsis signify a role of cysteinyl SH-groups in regulatory phosphorylation of plastid sigma factors. Phylogenetic and functional features of the plastid transcription kinase cpCK2 from Arabidopsis signify a role of cysteinyl SH-groups in regulatory phosphorylation of plastid sigma factors. FEBS J. (1742-464X) 2012 Türkeri H., Schweer J., Link G. in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Türkeri H., Schweer J., Link G. FEBS J. (1742-464X) 2012 o49935_sinal Sig1 protein protein sinal Sinapis alba (White mustard) Brassica hirta White mustard MATTAVIGLNAGKRLLSSSFYYSDVTEKFLSVNDLCSSQYHTRSGITAKKTSSSSSSNYSPSFPSSSRQTQSAKALKESVASSVQPWLPPGSDQELEEDDIDHSVEALLLLQRSMLEKQWNLSFEKTRKKVPVTCSGISARQRRIGAKKKTNVKAVSEVNFQNNLVKGYVKGVISDHVLSHAEVVRLSKKIKSGLRLDEHKSRLTDRLGCEPSDEQLAMSLKISRAELQAWLMECHLAREKLAMSNVRLVMSIAQRYDNMGAEMSDLVQGGLIGLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVDNSRTLRLPTHLHERLGLIRNAKLRLQEKGITPSIDRIAESLNMSQKKVRNATEAVSKIFSLDRDAFPSLNGLPGETHHSYIADNRLENNPWHGYDALALKDEVSKLISVTLGEREREIIRLYYGLDKECLTWEDISQRIGLSRERVRQVGLVALEKLKHAARKRKMEAMILKN mint AE15AF90DFD3AF20 csk2p_arath Casein kinase II subunit alpha-4, chloroplastic CKA4 Plastid-targeted casein kinase 2 alpha F21P24.13 At2g23070 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MALRPCTGFTISSLRNASAANNNLFSLLSFSSSSPAKRNLLLSSLQDNLRRFASSASLYRQHLRNQQQQHQQQQQSRVKEKSETLAQKIGKSIRRAGAPSKARVYADVNVVRPKDYWDYESLAVQWGVQDDYEVVRKVGRGKYSEVFEGIHATDNEKCVIKILKPVKKKKIKREIKILQNLCGGPNIVKLLDIVRDQQSKTPSLIFEHVNNKDFKVLYPTLSDYDVRYYIFELLKALDFCHSRGIMHRDVKPHNVMIDHEQRKLRLIDWGLAEFYHPGKEYNVRVASRYFKGPELLVDLQDYDYSLDLWSLGCMFAGMIFRKEPFFYGHDNYDQLVKIAKVLGTDELNAYLNKYRIELDPNLTSLVGRHSRKPWTKFINSENQHLAVPEAVDFVDKLLRYDHQERPTAKEAMAHPYFYPIRNAESSRTPRSQ 6DFCF7453B225E4A q9mth3_sinal RNA polymerase sigma factor sig2 protein protein sinal Sinapis alba (White mustard) Brassica hirta White mustard MSSCLLPNFKCQPDSFSVHFRTSYSVSKHSKGSAFFQPQCAVSTSPPLLTSTLDVAKLRLPSFDTDSDSRISDRPWTYTRTIGPSTEAKYLEALASETLLTSDEAVVAAAAAEAVALARAALKVAKDATLFGNSHNTKLSVSSAADKRSKWDQFTEKERAGILGHLAVSDTGVVSDRITAPGSLECNKEASDDLTPAEKQEVEKLSVSSAVRSTRQTERKARRVKGLEKTASGMQSSVKTGSSSRKKRVASQEIDHNDPLRYLRMTTSSSKLLTAREEQQLSEGIQDLLKLERLQAELTERCGHQPTFSQWASAAGVDKKTLRKRITHGTQCKDRMIKSNIRLVISIAKNYQGAGMDFQDLVQEGCRGLVRGAEKFDATKGFKFSTYAHWWIKQAVRKSLSDQSRMIRLPFHMVEATYRVKEARKQLYSETGKQPKNEEVAEATGLSMKRLMAVLLSPKPPRSLDQKIGINLNLKPSEVIADPEAETSEDILMKQFMRQDLDKVLDSLSTREKQVIRWRFGMEDGRMKTLQEIGETMGVSRERVRQIDTSAFRKLKNKKRNSHLQQYLVAQTKES mint 8670B6D65ABA9806 csk2p-sig1 1 SaSig1 2 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra predetermined predetermined participant predetermined enzyme target enzyme target substrate substrate neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cpCK2 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined phosphorylation phosphorylation reaction phosphorylation f2b mint csk2p-sig2 1 SaSig2 4 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra predetermined predetermined participant predetermined enzyme target enzyme target substrate substrate neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cpCK2 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined phosphorylation phosphorylation reaction phosphorylation f2b mint