IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Molecular basis of cross-species ACE2 interactions with SARS-CoV-2-like viruses of pangolin origin. Molecular basis of cross-species ACE2 interactions with SARS-CoV-2-like viruses of pangolin origin. The EMBO journal 2021 imex curation Niu S., Wang J., Bai B., Wu L., Zheng A., Chen Q., Du P., Han P., Zhang Y., Jia Y., Qiao C., Qi J., Tian WX., Wang HW., Wang Q., Fu Gao G. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus RBD sequences from Pangolin Spike proteins have been entered with sequence numbering related to N and C-terminal sequences of SARS2 virus RBD using Trembl identifiers from PDBe. The GISAID sequences are not publicly available. The Methods section states all Spike RBDs are "residues R319-F541", but these are the wrong amino acids at these locations for the Pangolin proteins with reference to the Trembl entries. Viral Uniprot identifiers were taken from PDBe. wenxiatian@126.com,hongweiwang@tsinghua.edu.cn,wangqihui@im.ac.cn,gaof@im.ac.cn Only protein-protein interactions imex curation in vitro In vitro facs fluorescence-activated cell sorting FACS Flow cytometry facs antibody detection identification by antibody antibody detection Niu S., Wang J., Bai B., Wu L., Zheng A., Chen Q., Du P., Han P., Zhang Y., Jia Y., Qiao C., Qi J., Tian WX., Wang HW., Wang Q., Fu Gao G. Virus - Publications including interactions involving viral proteins despite what the text says I have not made a FACS entry for the Intermediate horseshoe bat; it barely looks positive to me - if you disagree I will enter it. For 27+ species see pmid: 33020722 Supplementary Table 1. The Malay Pangolin sequence given from Wu et al, 2020a (pmid: 33020722) - XP_017505746 is obsolete; an identical sequence from a current entry (XP_017505752.1) has been used instead. The ACE2 entry for the Big-eared horseshoe bat has been taken from Trembl (E2DHI3), as it is not stated in the above reference. RBD sequences from Pangolin Spike proteins have been entered with sequence numbering related to N and C-terminal sequences of SARS2 virus RBD using Trembl identifiers from PDBe. The GISAID sequences are not publicly available. The Methods section states all Spike RBDs are "residues R319-F541", but these are the wrong amino acids at these locations for the Pangolin proteins with reference to the Trembl entries. Viral Uniprot identifiers were taken from PDBe. wenxiatian@126.com,hongweiwang@tsinghua.edu.cn,wangqihui@im.ac.cn,gaof@im.ac.cn Accepted 2021-AUG-03 AT 17:07 BST AT 17:07 BST by LUANA The EMBO journal 2021 Only protein-protein interactions imex curation imex curation a0a6m3g9r1_9betc Spike glycoprotein S E2 Peplomer protein protein protein Pangolin coronavirus Pangolin coronavirus MLFFFFLHFALVNSQCVNLTGRAAIQPSFTNSSQRGVYYPDTIFRSNTLVLSQGYFLPFYSNVSWYYALTKTNSAEKRVDNPVLDFKDGIYFAATEKSNIVRGWIFGTTLDNTSQSLLIVNNATNVIIKVCNFQFCYDPYLSGYYHNNKTWSTREFAVYSSYANCTFEYVSKSFMLDIAGKSGLFDTLREFVFRNVDGYFKIYSKYTPVNVNSNLPIGFSALEPLVEIPAGINITKFRTLLTIHRGDPMPNNGWTVFSAAYYVGYLAPRTFMLNYNENGTITDAVDCALDPLSEAKCTLKSLTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATTFASVYAWNRKRISNCVADYSVLYNSTSFSTFKCYGVSPTKLNDLCFTNVYADSFVVRGDEVRQIAPGQTGRIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFHPTNGVGYQPYRVVVLSFELLKAPATVCGPKQSTNLVKNKCVNFNFNGLTGTGVLTESSKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNTYECDIPIGAGICASYQTQTNSRSVSSQAIIAYTMSLGAENSVAYANNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSIECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPSQEKNFTTTPAICHEGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGSCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIIMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT 3AD969975DD0FAAE a0a6g6a1m4_9betc Spike glycoprotein S E2 Peplomer protein protein protein Pangolin coronavirus Pangolin coronavirus MFVFLFVLPLVSSQCVNLTTRTGIPPGYTNSSTRGVYYPDKVFRSSILHLTQDLFLPFFSNVTWFNTINYQGGFKKFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDARTQSLLIVNNATNVVIKVCEFQFCTDPFLGVYYHNNNKTWVENEFRVYSSANNCTFEYISQPFLMDLEGKQGNFKNLREFVFKNVDGYFKIYSKHTPIDLVRDLPRGFAALEPLVDLPIGINITRFQTLLALHRSYLTPGKLESGWTTGAAAYYVGYLQQRTFLLSYNQNGTITDAVDCSLDPLSETKCTLKSLTVEKGIYQTSNFRVQPTISIVRFPNITNLCPFGEVFNASKFASVYAWNRKRISNCVADYSVLYNSTSFSTFKCYGVSPTKLNDLCFTNVYADSFVVKGDEVRQIAPGQTGVIADYNYKLPDDFTGCVIAWNSVKQDALTGGNYGYLYRLFRKSKLKPFERDISTEIYQAGSTPCNGQVGLNCYYPLERYGFHPTTGVNYQPFRVVVLSFELLNGPATVCGPKLSTTLVKDKCVNFNFNGLTGTGVLTTSKKQFLPFQQFGRDISDTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPMAIHAEQLTPAWRVYSAGANVFQTRAGCLVGAEHVNNSYECDIPVGAGICASYHSMSSFRSVNQRSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSIECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHEGKAHFPREGVFVSNGTHWFITQRNFYEPQIITTDNTFVSGSCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIIMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT 3F0F7CDE3B8D3DF1 spike_sars2 Spike glycoprotein S E2 Peplomer protein 2 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT a0a3q7rat9_vulvu Angiotensin-converting enzyme ACE2 protein protein Red fox Vulpes vulpes MSGSSWLLLSLAALTAAQSTEDLVNTFLEKFNYEAEELSYQSSLASWDYNTNISDENVQKMNNAGAKWSAFYEEQSKLAKTYPLEEIQDSTVKRQLRALQHSGSSVLSADKNQRLNTILNSMSTIYSTGKACNPSNPQECLLLEPGLDDIMENSKDYNERLWAWEGWRSEVGKQLRPLYEEYVALKNEMARANNYEDYGDYWRGDYEEEWENGYNYSRNQLIDDVEHTFTQIMPLYQHLHAYVRTKLMDTYPSYISPTGCLPAHLLGDMWGRFWTNLYPLTVPFGQKPNIDVTNAMVNQSWDARKIFKEAEKFFVSVGLPNMTQGFWENSMLTEPSDSRKVVCHPTAWDLGKGDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPNHLKNIGLLPPSFFEDSETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKTWWEMKRNIVGVVEPVPHDETYCDPASLFHVANDYSFIRYYTRTIYQFQFQEALCQIAKHEGPLHKCDISNSSEAGQKLLEMLKLGKSKPWTYALEIVVGAKNMDVRPLLNYFEPLFTWLKEQNRNSFVGWNTDWSPYADQSIKVRISLKSALGEKAYEWNNNEMYLFRSSIAYAMRRYFSEVKKQTIPFVEDNVWVSDLKPRISFNFFVTSPGNVSDIIPRTEVEKAIRMYRGRINDVFRLDDNSLEFLGIQPTLGPSYEPPVTIWLIVFGVVMGVVVVGIVLLIFSGIRNRRKNDQARGEENPYASVDLSKGENNPGFQNVDDAQTSF A5276D1A33F36A55 ace2-s-125 1 5 tag fluorescence tag visualisation by fluorescence tag fluorescence antibody detection identification by antibody antibody detection unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mesau-bhk21 Mesocricetus auratus_BHK21 bhk-21 Cells from kidneys of five unsexed, one-day-old hamsters BHK-21/C13 cell 2 unspecified role unspecified role neutral component neutral component purified purified rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range gp67 signal sequence tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. physical association physical association 1, Fox number 1 ace2-s-126 1 3 unspecified role unspecified role neutral component neutral component purified purified rbd sufficient to bind sufficient binding region sufficient to bind range range range range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range gp67 signal sequence tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 5 tag fluorescence tag visualisation by fluorescence tag fluorescence antibody detection identification by antibody antibody detection unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mesau-bhk21 Mesocricetus auratus_BHK21 bhk-21 Cells from kidneys of five unsexed, one-day-old hamsters BHK-21/C13 cell physical association physical association 1, Fox number 2 ace2-s-127 1 4 unspecified role unspecified role neutral component neutral component purified purified rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range gp67 signal sequence tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 5 tag fluorescence tag visualisation by fluorescence tag fluorescence antibody detection identification by antibody antibody detection unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mesau-bhk21 Mesocricetus auratus_BHK21 bhk-21 Cells from kidneys of five unsexed, one-day-old hamsters BHK-21/C13 cell physical association physical association 1, Fox number 3