IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Molecular basis of immune evasion by the Delta and Kappa SARS-CoV-2 variants. Molecular basis of immune evasion by the Delta and Kappa SARS-CoV-2 variants. Science (New York, N.Y.) 2021 imex curation McCallum M, McCallum M., Walls AC., Sprouse KR., Bowen JE., Rosen LE., Dang HV., De Marco A., Franko N., Tilles SW., Logue J., Miranda MC., Ahlrichs M., Carter L., Snell G., Pizzuto MS., Chu HY., Van Voorhis WC., Corti D., Veesler D. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus ACE2 construct (Elisa): Supplement Methods refers to Ref. 64 - which refers to several constructs of ACE2 of varying lengths + different tags;none of which, naturally, correspond to the construct used here. Spike RBD_ I do not understand what aas the construct is stated to contain so have entered in fuzzy type ("boundaries of the construct are N-328-RFPN-331 and 528-KKST-531.")- there is NO "N" at residue 328. Biotinylated proteins; I have assumed the Avi-tag was biotinylated - although this is not stated. Kd/BLI + SPR values for mutants - I have entered as mutations of "no effect" when the text says "roughly comparable affinity to the wild-type RBD" or similar. dveesler@uw.edu Only protein-protein interactions imex curation in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection McCallum M, McCallum M., Walls AC., Sprouse KR., Bowen JE., Rosen LE., Dang HV., De Marco A., Franko N., Tilles SW., Logue J., Miranda MC., Ahlrichs M., Carter L., Snell G., Pizzuto MS., Chu HY., Van Voorhis WC., Corti D., Veesler D. Virus - Publications including interactions involving viral proteins ACE2 construct (Elisa): Supplement Methods refers to Ref. 64 - which refers to several constructs of ACE2 of varying lengths + different tags;none of which, naturally, correspond to the construct used here. Spike RBD_ I do not understand what aas the construct is stated to contain so have entered in fuzzy type ("boundaries of the construct are N-328-RFPN-331 and 528-KKST-531.")- there is NO "N" at residue 328. Biotinylated proteins; I have assumed the Avi-tag was biotinylated - although this is not stated. Kd/BLI + SPR values for mutants - I have entered as mutations of "no effect" when the text says "roughly comparable affinity to the wild-type RBD" or similar. dveesler@uw.edu Accepted 2021-NOV-30 AT 07:44 GMT AT 07:44 GMT by ORCHARD Science (New York, N.Y.) 2021 Only protein-protein interactions imex curation imex curation Molecular basis of immune evasion by the Delta and Kappa SARS-CoV-2 variants. Molecular basis of immune evasion by the Delta and Kappa SARS-CoV-2 variants. Science (New York, N.Y.) 2021 imex curation McCallum M, McCallum M., Walls AC., Sprouse KR., Bowen JE., Rosen LE., Dang HV., De Marco A., Franko N., Tilles SW., Logue J., Miranda MC., Ahlrichs M., Carter L., Snell G., Pizzuto MS., Chu HY., Van Voorhis WC., Corti D., Veesler D. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus ACE2 construct (Elisa): Supplement Methods refers to Ref. 64 - which refers to several constructs of ACE2 of varying lengths + different tags;none of which, naturally, correspond to the construct used here. Spike RBD_ I do not understand what aas the construct is stated to contain so have entered in fuzzy type ("boundaries of the construct are N-328-RFPN-331 and 528-KKST-531.")- there is NO "N" at residue 328. Biotinylated proteins; I have assumed the Avi-tag was biotinylated - although this is not stated. Kd/BLI + SPR values for mutants - I have entered as mutations of "no effect" when the text says "roughly comparable affinity to the wild-type RBD" or similar. dveesler@uw.edu Only protein-protein interactions imex curation in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined McCallum M, McCallum M., Walls AC., Sprouse KR., Bowen JE., Rosen LE., Dang HV., De Marco A., Franko N., Tilles SW., Logue J., Miranda MC., Ahlrichs M., Carter L., Snell G., Pizzuto MS., Chu HY., Van Voorhis WC., Corti D., Veesler D. Virus - Publications including interactions involving viral proteins ACE2 construct (Elisa): Supplement Methods refers to Ref. 64 - which refers to several constructs of ACE2 of varying lengths + different tags;none of which, naturally, correspond to the construct used here. Spike RBD_ I do not understand what aas the construct is stated to contain so have entered in fuzzy type ("boundaries of the construct are N-328-RFPN-331 and 528-KKST-531.")- there is NO "N" at residue 328. Biotinylated proteins; I have assumed the Avi-tag was biotinylated - although this is not stated. Kd/BLI + SPR values for mutants - I have entered as mutations of "no effect" when the text says "roughly comparable affinity to the wild-type RBD" or similar. dveesler@uw.edu Accepted 2021-NOV-30 AT 07:44 GMT AT 07:44 GMT by ORCHARD Science (New York, N.Y.) 2021 Only protein-protein interactions imex curation imex curation Molecular basis of immune evasion by the Delta and Kappa SARS-CoV-2 variants. Molecular basis of immune evasion by the Delta and Kappa SARS-CoV-2 variants. Science (New York, N.Y.) 2021 imex curation McCallum M, McCallum M., Walls AC., Sprouse KR., Bowen JE., Rosen LE., Dang HV., De Marco A., Franko N., Tilles SW., Logue J., Miranda MC., Ahlrichs M., Carter L., Snell G., Pizzuto MS., Chu HY., Van Voorhis WC., Corti D., Veesler D. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus ACE2 construct (Elisa): Supplement Methods refers to Ref. 64 - which refers to several constructs of ACE2 of varying lengths + different tags;none of which, naturally, correspond to the construct used here. Spike RBD_ I do not understand what aas the construct is stated to contain so have entered in fuzzy type ("boundaries of the construct are N-328-RFPN-331 and 528-KKST-531.")- there is NO "N" at residue 328. Biotinylated proteins; I have assumed the Avi-tag was biotinylated - although this is not stated. Kd/BLI + SPR values for mutants - I have entered as mutations of "no effect" when the text says "roughly comparable affinity to the wild-type RBD" or similar. dveesler@uw.edu Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined McCallum M, McCallum M., Walls AC., Sprouse KR., Bowen JE., Rosen LE., Dang HV., De Marco A., Franko N., Tilles SW., Logue J., Miranda MC., Ahlrichs M., Carter L., Snell G., Pizzuto MS., Chu HY., Van Voorhis WC., Corti D., Veesler D. Virus - Publications including interactions involving viral proteins ACE2 construct (Elisa): Supplement Methods refers to Ref. 64 - which refers to several constructs of ACE2 of varying lengths + different tags;none of which, naturally, correspond to the construct used here. Spike RBD_ I do not understand what aas the construct is stated to contain so have entered in fuzzy type ("boundaries of the construct are N-328-RFPN-331 and 528-KKST-531.")- there is NO "N" at residue 328. Biotinylated proteins; I have assumed the Avi-tag was biotinylated - although this is not stated. Kd/BLI + SPR values for mutants - I have entered as mutations of "no effect" when the text says "roughly comparable affinity to the wild-type RBD" or similar. dveesler@uw.edu Accepted 2021-NOV-30 AT 07:44 GMT AT 07:44 GMT by ORCHARD Science (New York, N.Y.) 2021 Only protein-protein interactions imex curation imex curation ace2_human Angiotensin-converting enzyme 2 ACE2 UNQ868/PRO1885 Angiotensin-converting enzyme homolog Metalloprotease MPROT15 Angiotensin-converting enzyme-related carboxypeptidase protein protein human Homo sapiens Human MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSIWLIVFGVVMGVIVVGIVILIFTGIRDRKKKNKARSGENPYASIDISKGENNPGFQNTDDVQTSF mint Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses 8EE6EB0A931550E8 spike_sars2 Spike glycoprotein S E2 Peplomer protein 2 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT ace2-s-213 1 4 unspecified role unspecified role bait bait purified purified avi-tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range signal peptide sufficient to bind sufficient binding region sufficient to bind n-term range n-terminal range n-term range n-term range n-terminal range n-term range partial ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 5 unspecified role unspecified role prey prey purified purified rbd sufficient to bind sufficient binding region sufficient to bind range range range range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range avi-tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mu-phosphatase signal peptide tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P0DTC2:p.Asn501Tyr mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N Y Thu Jan 06 19:45:34 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn501Tyr' P0DTC2:p.[Leu452Arg;Glu484Gln] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L R certain certain sequence position certain certain certain sequence position certain E Q Thu Jan 06 19:51:58 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu452Arg;Glu484Gln]' P0DTC2:p.[Leu452Arg;Thr478Lys] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L R certain certain sequence position certain certain certain sequence position certain T K Thu Jan 06 19:45:13 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu452Arg;Thr478Lys]' P0DTC2:p.[Lys417Asn;Leu452Arg;Thr478Lys] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L R certain certain sequence position certain certain certain sequence position certain T K certain certain sequence position certain certain certain sequence position certain K N Thu Jan 06 19:06:56 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys417Asn;Leu452Arg;Thr478Lys]' human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction Fig. 2G, fig. S4, and table S4 s-ace2-125 3 4 unspecified role unspecified role prey prey purified purified avi-tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range signal peptide sufficient to bind sufficient binding region sufficient to bind n-term range n-terminal range n-term range n-term range n-terminal range n-term range partial ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 5 unspecified role unspecified role bait bait purified purified rbd sufficient to bind sufficient binding region sufficient to bind range range range range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mu-phosphatase signal peptide tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P0DTC2:p.Asn501Tyr mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N Y Thu Jan 06 18:26:07 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn501Tyr' P0DTC2:p.[Leu452Arg;Glu484Gln] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L R certain certain sequence position certain certain certain sequence position certain E Q Thu Jan 06 19:44:07 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu452Arg;Glu484Gln]' P0DTC2:p.[Leu452Arg;Thr478Lys] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L R certain certain sequence position certain certain certain sequence position certain T K Thu Jan 06 18:36:18 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu452Arg;Thr478Lys]' P0DTC2:p.[Lys417Asn;Leu452Arg;Thr478Lys] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L R certain certain sequence position certain certain certain sequence position certain T K certain certain sequence position certain certain certain sequence position certain K N Thu Jan 06 18:28:31 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys417Asn;Leu452Arg;Thr478Lys]' human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction Fig. 2H, fig. S4, and table S4 ace2-s-215 2 4 unspecified role unspecified role prey prey purified purified avi-tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range signal peptide sufficient to bind sufficient binding region sufficient to bind n-term range n-terminal range n-term range n-term range n-terminal range n-term range partial ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 5 unspecified role unspecified role bait bait purified purified rbd sufficient to bind sufficient binding region sufficient to bind range range range range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mu-phosphatase signal peptide tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P0DTC2:p.Asn501Tyr mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N Y Thu Jan 06 20:23:10 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn501Tyr' P0DTC2:p.[Leu452Arg;Glu484Gln] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L R certain certain sequence position certain certain certain sequence position certain E Q Thu Jan 06 19:00:06 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu452Arg;Glu484Gln]' P0DTC2:p.[Leu452Arg;Thr478Lys] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L R certain certain sequence position certain certain certain sequence position certain T K Thu Jan 06 19:22:57 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu452Arg;Thr478Lys]' P0DTC2:p.[Lys417Asn;Leu452Arg;Thr478Lys] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L R certain certain sequence position certain certain certain sequence position certain T K certain certain sequence position certain certain certain sequence position certain K N Thu Jan 06 18:38:32 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys417Asn;Leu452Arg;Thr478Lys]' Fri Jan 28 17:36:56 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'P0DTC2:p.[Leu452Arg;Thr478Lys]' human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction Fig. 2I, fig. S4, and table S4