IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The SARS-CoV-2 main protease Mpro causes microvascular brain pathology by cleaving NEMO in brain endothelial cells. The SARS-CoV-2 main protease Mpro causes microvascular brain pathology by cleaving NEMO in brain endothelial cells. Nature neuroscience 2021 imex curation Wenzel J., Lampe J., Müller-Fielitz H., Schuster R., Zille M., Müller K., Krohn M., Körbelin J., Zhang L., Özorhan Ü., Neve V., Wagner JUG., Bojkova D., Shumliakivska M., Jiang Y., Fähnrich A., Ott F., Sencio V., Robil C., Pfefferle S., Sauve F., Coêlho CFF., Franz J., Spiecker F., Lembrich B., Binder S., Feller N., König P., Busch H., Collin L., Villaseñor R., Jöhren O., Altmeppen HC., Pasparakis M., Dimmeler S., Cinatl J., Püschel K., Zelic M., Ofengeim D., Stadelmann C., Trottein F., Nogueiras R., Hilgenfeld R., Glatzel M., Prevot V., Schwaninger M. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus markus.schwaninger@uni-luebeck.de Only protein-protein interactions imex curation in vitro In vitro protease assay protease assay experimental particp experimental participant identification experimental particp Wenzel J., Lampe J., Müller-Fielitz H., Schuster R., Zille M., Müller K., Krohn M., Körbelin J., Zhang L., Özorhan Ü., Neve V., Wagner JUG., Bojkova D., Shumliakivska M., Jiang Y., Fähnrich A., Ott F., Sencio V., Robil C., Pfefferle S., Sauve F., Coêlho CFF., Franz J., Spiecker F., Lembrich B., Binder S., Feller N., König P., Busch H., Collin L., Villaseñor R., Jöhren O., Altmeppen HC., Pasparakis M., Dimmeler S., Cinatl J., Püschel K., Zelic M., Ofengeim D., Stadelmann C., Trottein F., Nogueiras R., Hilgenfeld R., Glatzel M., Prevot V., Schwaninger M. Virus - Publications including interactions involving viral proteins markus.schwaninger@uni-luebeck.de Accepted 2021-DEC-14 AT 15:33 GMT AT 15:33 GMT by SIMONA Nature neuroscience 2021 Only protein-protein interactions imex curation imex curation p0dtd1-pro_0000449623 3C-like proteinase nsp5 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV SGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQ 3264 3569 nemo_mouse NF-kappa-B essential modulator NF-kappa-B essential modifier Inhibitor of nuclear factor kappa-B kinase subunit gamma IkB kinase-associated protein 1 Ikbkg Nemo protein protein mouse Mus musculus Mouse MNKHPWKNQLSEMVQPSGGPAEDQDMLGEESSLGKPAMLHLPSEQGTPETLQRCLEENQELRDAIRQSNQMLRERCEELLHFQVSQREEKEFLMCKFQEARKLVERLSLEKLDLRSQREQALKELEQLKKCQQQMAEDKASVKAQVTSLLGELQESQSRLEAATKDRQALEGRIRAVSEQVRQLESEREVLQQQHSVQVDQLRMQNQSVEAALRMERQAASEEKRKLAQLQAAYHQLFQDYDSHIKSSKGMQLEDLRQQLQQAEEALVAKQELIDKLKEEAEQHKIVMETVPVLKAQADIYKADFQAERHAREKLVEKKEYLQEQLEQLQREFNKLKVGCHESARIEDMRKRHVETPQPPLLPAPAHHSFHLALSNQRRSPPEEPPDFCCPKCQYQAPDMDTLQIHVMECIE DIP protein O88522 original sequence version: 115 DIP protein O88522 original sequence version: 63 DIP protein O88522 original sequence version: 91 DIP protein O88522 original sequence version: 90 DIP protein O88522 original sequence version: 104 DIP protein O88522 original sequence version: 138 66C693C857A2D5E6 q9y6k9-1 NF-kappa-B essential modulator FIP-3 IkB kinase-associated protein 1 Inhibitor of nuclear factor kappa-B kinase subunit gamma NF-kappa-B essential modifier IKBKG FIP3 NEMO protein protein human Homo sapiens Human MNRHLWKSQLCEMVQPSGGPAADQDVLGEESPLGKPAMLHLPSEQGAPETLQRCLEENQELRDAIRQSNQILRERCEELLHFQASQREEKEFLMCKFQEARKLVERLGLEKLDLKRQKEQALREVEHLKRCQQQMAEDKASVKAQVTSLLGELQESQSRLEAATKECQALEGRARAASEQARQLESEREALQQQHSVQVDQLRMQGQSVEAALRMERQAASEEKRKLAQLQVAYHQLFQEYDNHIKSSVVGSERKRGMQLEDLKQQLQQAEEALVAKQEVIDKLKEEAEQHKIVMETVPVLKAQADIYKADFQAERQAREKLAEKKELLQEQLEQLQREYSKLKASCQESARIEDMRKRHVEVSQAPLPPAPAYLSSPLALPSQRRSPPEEPPDFCCPKCQYQAPDMDTLQIHVMECIE 322D1037881447FF ikbkg-rep-2 1 2 enzyme enzyme neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 4 sequence tag sequence tag identification sequence tag western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp enzyme target enzyme target substrate substrate neutral component neutral component purified purified gln83 biological feature biological feature certain certain sequence position certain certain certain sequence position certain resulting-cleavage resulting-cleavage gln205 biological feature biological feature certain certain sequence position certain certain certain sequence position certain resulting-cleavage resulting-cleavage gln231 biological feature biological feature certain certain sequence position certain certain certain sequence position certain resulting-cleavage resulting-cleavage gln304 biological feature biological feature certain certain sequence position certain certain certain sequence position certain resulting-cleavage resulting-cleavage gln313 biological feature biological feature certain certain sequence position certain certain certain sequence position certain resulting-cleavage resulting-cleavage n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) protein cleavage protein cleavage 3 A/D/E, Supp. figure 2 ikbkg-rep-3 1 2 enzyme enzyme neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 4 sequence tag sequence tag identification sequence tag experimental particp experimental participant identification experimental particp enzyme target enzyme target substrate substrate neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gln231 biological feature biological feature certain certain sequence position certain certain certain sequence position certain resulting-cleavage resulting-cleavage Q9Y6K9-1:p.Gln231Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain Q A chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage 3F, Supp. figure 3 ikbkg-rep-4 1 2 enzyme enzyme neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 3 sequence tag sequence tag identification sequence tag experimental particp experimental participant identification experimental particp enzyme target enzyme target substrate substrate neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gln231 biological feature biological feature certain certain sequence position certain certain certain sequence position certain resulting-cleavage resulting-cleavage chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage 3F, Supp. figure 3 B/C