IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A dual-role of SARS-CoV-2 nucleocapsid protein in regulating innate immune response. A dual-role of SARS-CoV-2 nucleocapsid protein in regulating innate immune response. Signal transduction and targeted therapy 2021 imex curation Zhao Y., Sui L., Wu P., Wang W., Wang Z., Yu Y., Hou Z., Tan G., Liu Q., Wang G., Zhao Y., Sui L., Wu P., Wang W., Wang Z., Yu Y., Hou Z., Tan G., Liu Q., Wang G. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus RIG1 is of Human origin as the Public Protein/Plasmid Library (http://www.geneppl.com) only appears to stock human plasmids of DDX58. GST tags are N-terminal as shown in figure 6D. tgy0425@jlu.edu.cn,liuquan1973@hotmail.com,qing@jlu.edu.cn Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Zhao Y., Sui L., Wu P., Wang W., Wang Z., Yu Y., Hou Z., Tan G., Liu Q., Wang G., Zhao Y., Sui L., Wu P., Wang W., Wang Z., Yu Y., Hou Z., Tan G., Liu Q., Wang G. Virus - Publications including interactions involving viral proteins RIG1 is of Human origin as the Public Protein/Plasmid Library (http://www.geneppl.com) only appears to stock human plasmids of DDX58. GST tags are N-terminal as shown in figure 6D. tgy0425@jlu.edu.cn,liuquan1973@hotmail.com,qing@jlu.edu.cn Accepted 2021-OCT-22 AT 09:39 BST AT 09:39 BST by LUANA Signal transduction and targeted therapy 2021 Only protein-protein interactions imex curation imex curation A dual-role of SARS-CoV-2 nucleocapsid protein in regulating innate immune response. A dual-role of SARS-CoV-2 nucleocapsid protein in regulating innate immune response. Signal transduction and targeted therapy 2021 imex curation Zhao Y., Sui L., Wu P., Wang W., Wang Z., Yu Y., Hou Z., Tan G., Liu Q., Wang G., Zhao Y., Sui L., Wu P., Wang W., Wang Z., Yu Y., Hou Z., Tan G., Liu Q., Wang G. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus RIG1 is of Human origin as the Public Protein/Plasmid Library (http://www.geneppl.com) only appears to stock human plasmids of DDX58. GST tags are N-terminal as shown in figure 6D. tgy0425@jlu.edu.cn,liuquan1973@hotmail.com,qing@jlu.edu.cn Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Zhao Y., Sui L., Wu P., Wang W., Wang Z., Yu Y., Hou Z., Tan G., Liu Q., Wang G., Zhao Y., Sui L., Wu P., Wang W., Wang Z., Yu Y., Hou Z., Tan G., Liu Q., Wang G. Virus - Publications including interactions involving viral proteins RIG1 is of Human origin as the Public Protein/Plasmid Library (http://www.geneppl.com) only appears to stock human plasmids of DDX58. GST tags are N-terminal as shown in figure 6D. tgy0425@jlu.edu.cn,liuquan1973@hotmail.com,qing@jlu.edu.cn Accepted 2021-OCT-22 AT 09:39 BST AT 09:39 BST by LUANA Signal transduction and targeted therapy 2021 Only protein-protein interactions imex curation imex curation A dual-role of SARS-CoV-2 nucleocapsid protein in regulating innate immune response. A dual-role of SARS-CoV-2 nucleocapsid protein in regulating innate immune response. Signal transduction and targeted therapy 2021 imex curation Zhao Y., Sui L., Wu P., Wang W., Wang Z., Yu Y., Hou Z., Tan G., Liu Q., Wang G., Zhao Y., Sui L., Wu P., Wang W., Wang Z., Yu Y., Hou Z., Tan G., Liu Q., Wang G. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus RIG1 is of Human origin as the Public Protein/Plasmid Library (http://www.geneppl.com) only appears to stock human plasmids of DDX58. GST tags are N-terminal as shown in figure 6D. tgy0425@jlu.edu.cn,liuquan1973@hotmail.com,qing@jlu.edu.cn Only protein-protein interactions imex curation human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Zhao Y., Sui L., Wu P., Wang W., Wang Z., Yu Y., Hou Z., Tan G., Liu Q., Wang G., Zhao Y., Sui L., Wu P., Wang W., Wang Z., Yu Y., Hou Z., Tan G., Liu Q., Wang G. Virus - Publications including interactions involving viral proteins WT and sg25 cells were used: sg25 cells are TRIM25 knockout cells derived from HepG2 cell line. RIG1 is of Human origin as the Public Protein/Plasmid Library (http://www.geneppl.com) only appears to stock human plasmids of DDX58. GST tags are N-terminal as shown in figure 6D. tgy0425@jlu.edu.cn,liuquan1973@hotmail.com,qing@jlu.edu.cn Accepted 2021-OCT-22 AT 09:39 BST AT 09:39 BST by LUANA Signal transduction and targeted therapy 2021 Only protein-protein interactions imex curation imex curation A dual-role of SARS-CoV-2 nucleocapsid protein in regulating innate immune response. A dual-role of SARS-CoV-2 nucleocapsid protein in regulating innate immune response. Signal transduction and targeted therapy 2021 imex curation Zhao Y., Sui L., Wu P., Wang W., Wang Z., Yu Y., Hou Z., Tan G., Liu Q., Wang G., Zhao Y., Sui L., Wu P., Wang W., Wang Z., Yu Y., Hou Z., Tan G., Liu Q., Wang G. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus RIG1 is of Human origin as the Public Protein/Plasmid Library (http://www.geneppl.com) only appears to stock human plasmids of DDX58. GST tags are N-terminal as shown in figure 6D. tgy0425@jlu.edu.cn,liuquan1973@hotmail.com,qing@jlu.edu.cn Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Zhao Y., Sui L., Wu P., Wang W., Wang Z., Yu Y., Hou Z., Tan G., Liu Q., Wang G., Zhao Y., Sui L., Wu P., Wang W., Wang Z., Yu Y., Hou Z., Tan G., Liu Q., Wang G. Virus - Publications including interactions involving viral proteins RIG1 is of Human origin as the Public Protein/Plasmid Library (http://www.geneppl.com) only appears to stock human plasmids of DDX58. GST tags are N-terminal as shown in figure 6D. tgy0425@jlu.edu.cn,liuquan1973@hotmail.com,qing@jlu.edu.cn Accepted 2021-OCT-22 AT 09:39 BST AT 09:39 BST by LUANA Signal transduction and targeted therapy 2021 Only protein-protein interactions imex curation imex curation ncap_sars2 Nucleoprotein N Nucleocapsid protein protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA stat2_human Signal transducer and activator of transcription 2 p113 STAT2 protein protein human Homo sapiens Human MAQWEMLQNLDSPFQDQLHQLYSHSLLPVDIRQYLAVWIEDQNWQEAALGSDDSKATMLFFHFLDQLNYECGRCSQDPESLLLQHNLRKFCRDIQPFSQDPTQLAEMIFNLLLEEKRILIQAQRAQLEQGEPVLETPVESQQHEIESRILDLRAMMEKLVKSISQLKDQQDVFCFRYKIQAKGKTPSLDPHQTKEQKILQETLNELDKRRKEVLDASKALLGRLTTLIELLLPKLEEWKAQQQKACIRAPIDHGLEQLETWFTAGAKLLFHLRQLLKELKGLSCLVSYQDDPLTKGVDLRNAQVTELLQRLLHRAFVVETQPCMPQTPHRPLILKTGSKFTVRTRLLVRLQEGNESLTVEVSIDRNPPQLQGFRKFNILTSNQKTLTPEKGQSQGLIWDFGYLTLVEQRSGGSGKGSNKGPLGVTEELHIISFTVKYTYQGLKQELKTDTLPVVIISNMNQLSIAWASVLWFNLLSPNLQNQQFFSNPPKAPWSLLGPALSWQFSSYVGRGLNSDQLSMLRNKLFGQNCRTEDPLLSWADFTKRESPPGKLPFWTWLDKILELVHDHLKDLWNDGRIMGFVSRSQERRLLKKTMSGTFLLRFSESSEGGITCSWVEHQDDDKVLIYSVQPYTKEVLQSLPLTEIIRHYQLLTEENIPENPLRFLYPRIPRDEAFGCYYQEKVNLQERRKYLKHRLIVVSNRQVDELQQPLELKPEPELESLELELGLVPEPELSLDLEPLLKAGLDLGPELESVLESTLEPVIEPTLCMVSQTVPEPDQGPVSQPVPEPDLPCDLRHLNTEPMEIFRNCVKIEEIMPNGDPLLAGQNTVDEVYVSRPSHFYTDGPLMPSDF DIP protein P52630 original sequence version: 116 E4C74674CB7A3215 ddx58_human Antiviral innate immune response receptor RIG-I DEAD box protein 58 Retinoic acid-inducible gene 1 protein DDX58 Probable ATP-dependent RNA helicase DDX58 Retinoic acid-inducible gene I protein RIG-I-like receptor 1 protein protein human Homo sapiens Human MTTEQRRSLQAFQDYIRKTLDPTYILSYMAPWFREEEVQYIQAEKNNKGPMEAATLFLKFLLELQEEGWFRGFLDALDHAGYSGLYEAIESWDFKKIEKLEEYRLLLKRLQPEFKTRIIPTDIISDLSECLINQECEEILQICSTKGMMAGAEKLVECLLRSDKENWPKTLKLALEKERNKFSELWIVEKGIKDVETEDLEDKMETSDIQIFYQEDPECQNLSENSCPPSEVSDTNLYSPFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKGKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENNDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYLDQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIATVKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRICKDLENLSQIQNREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYNDALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELESVSRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEGNPKLSFLKPGILTGRGKTNQNTGMTLPAQKCILDAFKASGDHNILIATSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTSNAGVIEKEQINMYKEKMMNDSILRLQTWDEAVFREKILHIQTHEKFIRDSQEKPKPVPDKENKKLLCRKCKALACYTADVRVIEECHYTVLGDAFKECFVSRPHPKPKQFSSFEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATGVQTLYSKWKDFHFEKIPFDPAEMSK BF0D501C395BAE25 ncap_sars Nucleoprotein N 9a Nucleocapsid protein protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MSDNGPQSNQRSAPRITFGGPTDSTDNNQNGGRNGARPKQRRPQGLPNNTASWFTALTQHGKEELRFPRGQGVPINTNSGPDDQIGYYRRATRRVRGGDGKMKELSPRWYFYYLGTGPEASLPYGANKEGIVWVATEGALNTPKDHIGTRNPNNNAATVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRGNSRNSTPGSSRGNSPARMASGGGETALALLLLDRLNQLESKVSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKQYNVTQAFGRRGPEQTQGNFGDQDLIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYHGAIKLDDKDPQFKDNVILLNKHIDAYKTFPPTEPKKDKKKKTDEAQPLPQRQKKQPTVTLLPAADMDDFSRQLQNSMSGASADSTQA mint Major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid 43FC8750F1253034 tri25_human E3 ubiquitin/ISG15 ligase TRIM25 TRIM25 EFP RNF147 ZNF147 Zinc finger protein 147 Estrogen-responsive finger protein RING finger protein 147 Ubiquitin/ISG15-conjugating enzyme TRIM25 Tripartite motif-containing protein 25 RING-type E3 ubiquitin transferase RING-type E3 ubiquitin transferase TRIM25 protein protein human Homo sapiens Human MAELCPLAEELSCSICLEPFKEPVTTPCGHNFCGSCLNETWAVQGSPYLCPQCRAVYQARPQLHKNTVLCNVVEQFLQADLAREPPADVWTPPARASAPSPNAQVACDHCLKEAAVKTCLVCMASFCQEHLQPHFDSPAFQDHPLQPPVRDLLRRKCSQHNRLREFFCPEHSECICHICLVEHKTCSPASLSQASADLEATLRHKLTVMYSQINGASRALDDVRNRQQDVRMTANRKVEQLQQEYTEMKALLDASETTSTRKIKEEEKRVNSKFDTIYQILLKKKSEIQTLKEEIEQSLTKRDEFEFLEKASKLRGISTKPVYIPEVELNHKLIKGIHQSTIDLKNELKQCIGRLQEPTPSSGDPGEHDPASTHKSTRPVKKVSKEEKKSKKPPPVPALPSKLPTFGAPEQLVDLKQAGLEAAAKATSSHPNSTSLKAKVLETFLAKSRPELLEYYIKVILDYNTAHNKVALSECYTVASVAEMPQNYRPHPQRFTYCSQVLGLHCYKKGIHYWEVELQKNNFCGVGICYGSMNRQGPESRLGRNSASWCVEWFNTKISAWHNNVEKTLPSTKATRVGVLLNCDHGFVIFFAVADKVHLMYKFRVDFTEALYPAFWVFSAGATLSICSPK EB0AB353F0AD4C80 ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG n-stat2-3 4 6 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3E n-trim25-3 1 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 5A n-trim25-4 1 9 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 5B trim25-n-4 2 9 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Spry domain sufficient to bind sufficient binding region sufficient to bind range range range range 5 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization 5C, 7H, Supp. 7D spry domain: the 610 value was taken from a Blat of the only primer for the SPRY domain that was long enough to get a result. n-stat2-2 4 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 5D ddx58-n-5 1 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 5E ddx58-n-6 1 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 inhibitor inhibitor prey prey over-expressed over expressed level over-expressed n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association 5 E, 6C The interaction of TRIM25 with RIG-I was inhibited by 2N. Overexpression of TRIM25 could rescue the suppression of RIG-I ubiquitination caused by 2N. ddx58-n-7 1 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 inhibitor inhibitor prey prey over-expressed over expressed level over-expressed n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association 5F The interaction of TRIM25 with RIG-I was inhibited by 2N. ddx58-n-8 1 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 5F The interaction of TRIM25 with RIG-I was inhibited by 2N. ddx58-n-9 2 7 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 5G The interaction of TRIM25 with RIG-I was inhibited by 2N. "N" protein -P0DTC9 ddx58-ubiq_human-1 2 7 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 6B "N" protein -P0DTC9 I am considering Ubiquitin as a "protein" here as it not necessarily shown to be a PTM here. ddx58-n-10 1 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 6 A/C, supp. figure 5D. 2N inhibits RIG-I ubiquitination. ddx58-n-11 3 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 6 D 2N inhibits RIG-I ubiquitination. Weak interaction. trim25-n-5 1 9 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range CTD region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain NTD region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 7A/D/E/I, Supplementary Fig. S6a, b. 2N can interact with both the SPRY- and RING-deleted TRIM25 truncations with the lower binding level than that of full-length TRIM25, indicating that 2N can interact with the SPRY and other domains of TRIM25, including the RING-finger domain. Figure 7I - I have not attempted to map which domains of TRIM25 interact as the possible ranges of the domains are quite variable (interpro), and the isolated SPRY domain does not appear to interact at all. Different results are seen for what I think is the same interaction in each figure- Supp. figs and 7I. trim25-n-6 1 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 7F Other regions of the N protein also bound weekly - notably aas 1-175 and 252-360. trim25-n-7 1 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level Spry domain required to bind necessary binding region required to bind range range range range region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supplementary Fig. S6C Only the SPRY domain of TRIM25 is stated to be responsible for the interaction with SARS-CoV N. However a deletion construct not containing the SPRY domain also appears to bind weakly. spry domain: 610 figure taken from Blat of only primer that gave a result. n-trim25-5 2 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Spry domain sufficient to bind sufficient binding region sufficient to bind range range range range colocalization colocalization Supp. figure 6D spry domain: the 610 value was taken from a Blat of the only primer for the SPRY domain that was long enough to get a result.