IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Molecular determinants for regulation of G3BP1/2 phase separation by the SARS-CoV-2 nucleocapsid protein. Molecular determinants for regulation of G3BP1/2 phase separation by the SARS-CoV-2 nucleocapsid protein. Cell discovery 2021 imex curation Huang W., Ju X., Tian M., Li X., Yu Y., Sun Q., Ding Q., Jia D. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus Most LLPS + Turbidity assays not added as i think the interaction is between G3BP1 and RNA AND/OR ficoll (both are often present and no sequences or ID for either);presumably cleavage of MBP tag starts the LLPS reaction, although there is nothing in Ref 2 +3 giving any info about this. qding@tsinghua.edu.cn, Jiada@scu.edu.cn Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Huang W., Ju X., Tian M., Li X., Yu Y., Sun Q., Ding Q., Jia D. Virus - Publications including interactions involving viral proteins The expression system for "Bait" proteins is not stated. According to Pmid:22513087 (traced back from Supplementary Ref. 1) these proteins are purified. Most LLPS + Turbidity assays not added as i think the interaction is between G3BP1 and RNA AND/OR ficoll (both are often present and no sequences or ID for either);presumably cleavage of MBP tag starts the LLPS reaction, although there is nothing in Ref 2 +3 giving any info about this. qding@tsinghua.edu.cn, Jiada@scu.edu.cn Accepted 2021-OCT-18 AT 06:07 BST AT 06:07 BST by KALPANAP Cell discovery 2021 Only protein-protein interactions imex curation imex curation Molecular determinants for regulation of G3BP1/2 phase separation by the SARS-CoV-2 nucleocapsid protein. Molecular determinants for regulation of G3BP1/2 phase separation by the SARS-CoV-2 nucleocapsid protein. Cell discovery 2021 imex curation Huang W., Ju X., Tian M., Li X., Yu Y., Sun Q., Ding Q., Jia D. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus Most LLPS + Turbidity assays not added as i think the interaction is between G3BP1 and RNA AND/OR ficoll (both are often present and no sequences or ID for either);presumably cleavage of MBP tag starts the LLPS reaction, although there is nothing in Ref 2 +3 giving any info about this. qding@tsinghua.edu.cn, Jiada@scu.edu.cn Only protein-protein interactions imex curation in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Huang W., Ju X., Tian M., Li X., Yu Y., Sun Q., Ding Q., Jia D. Virus - Publications including interactions involving viral proteins The expression system for "Bait" proteins is not stated. According to Pmid:22513087 (traced back from Supplementary Ref. 1) these proteins are purified. Most LLPS + Turbidity assays not added as i think the interaction is between G3BP1 and RNA AND/OR ficoll (both are often present and no sequences or ID for either);presumably cleavage of MBP tag starts the LLPS reaction, although there is nothing in Ref 2 +3 giving any info about this. qding@tsinghua.edu.cn, Jiada@scu.edu.cn Accepted 2021-OCT-18 AT 06:07 BST AT 06:07 BST by KALPANAP Cell discovery 2021 Only protein-protein interactions imex curation imex curation Molecular determinants for regulation of G3BP1/2 phase separation by the SARS-CoV-2 nucleocapsid protein. Molecular determinants for regulation of G3BP1/2 phase separation by the SARS-CoV-2 nucleocapsid protein. Cell discovery 2021 imex curation Huang W., Ju X., Tian M., Li X., Yu Y., Sun Q., Ding Q., Jia D. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus Most LLPS + Turbidity assays not added as i think the interaction is between G3BP1 and RNA AND/OR ficoll (both are often present and no sequences or ID for either);presumably cleavage of MBP tag starts the LLPS reaction, although there is nothing in Ref 2 +3 giving any info about this. qding@tsinghua.edu.cn, Jiada@scu.edu.cn Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Huang W., Ju X., Tian M., Li X., Yu Y., Sun Q., Ding Q., Jia D. Virus - Publications including interactions involving viral proteins Most LLPS + Turbidity assays not added as i think the interaction is between G3BP1 and RNA AND/OR ficoll (both are often present and no sequences or ID for either);presumably cleavage of MBP tag starts the LLPS reaction, although there is nothing in Ref 2 +3 giving any info about this. qding@tsinghua.edu.cn, Jiada@scu.edu.cn Accepted 2021-OCT-18 AT 06:07 BST AT 06:07 BST by KALPANAP Cell discovery 2021 Only protein-protein interactions imex curation imex curation Molecular determinants for regulation of G3BP1/2 phase separation by the SARS-CoV-2 nucleocapsid protein. Molecular determinants for regulation of G3BP1/2 phase separation by the SARS-CoV-2 nucleocapsid protein. Cell discovery 2021 imex curation Huang W., Ju X., Tian M., Li X., Yu Y., Sun Q., Ding Q., Jia D. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus Most LLPS + Turbidity assays not added as i think the interaction is between G3BP1 and RNA AND/OR ficoll (both are often present and no sequences or ID for either);presumably cleavage of MBP tag starts the LLPS reaction, although there is nothing in Ref 2 +3 giving any info about this. qding@tsinghua.edu.cn, Jiada@scu.edu.cn Only protein-protein interactions imex curation in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Huang W., Ju X., Tian M., Li X., Yu Y., Sun Q., Ding Q., Jia D. Virus - Publications including interactions involving viral proteins The expression system for "Bait" proteins is not stated. According to Pmid:22513087 (traced back from Supplementary Ref. 1) these proteins are purified. Most LLPS + Turbidity assays not added as i think the interaction is between G3BP1 and RNA AND/OR ficoll (both are often present and no sequences or ID for either);presumably cleavage of MBP tag starts the LLPS reaction, although there is nothing in Ref 2 +3 giving any info about this. qding@tsinghua.edu.cn, Jiada@scu.edu.cn Accepted 2021-OCT-18 AT 06:07 BST AT 06:07 BST by KALPANAP Cell discovery 2021 Only protein-protein interactions imex curation imex curation g3bp2_human Ras GTPase-activating protein-binding protein 2 GAP SH3 domain-binding protein 2 G3BP2 KIAA0660 protein protein human Homo sapiens Human MVMEKPSPLLVGREFVRQYYTLLNKAPEYLHRFYGRNSSYVHGGVDASGKPQEAVYGQNDIHHKVLSLNFSECHTKIRHVDAHATLSDGVVVQVMGLLSNSGQPERKFMQTFVLAPEGSVPNKFYVHNDMFRYEDEVFGDSEPELDEESEDEVEEEQEERQPSPEPVQENANSGYYEAHPVTNGIEEPLEESSHEPEPEPESETKTEELKPQVEEKNLEELEEKSTTPPPAEPVSLPQEPPKAFSWASVTSKNLPPSGTVSSSGIPPHVKAPVSQPRVEAKPEVQSQPPRVREQRPRERPGFPPRGPRPGRGDMEQNDSDNRRIIRYPDSHQLFVGNLPHDIDENELKEFFMSFGNVVELRINTKGVGGKLPNFGFVVFDDSEPVQRILIAKPIMFRGEVRLNVEEKKTRAARERETRGGGDDRRDIRRNDRGPGGPRGIVGGGMMRDRDGRGPPPRGGMAQKLGSGRGTGQMEGRFTGQRR DIP protein Q9UN86 original sequence version: 147 74B8EA43C0560229 ncap_sars Nucleoprotein N 9a Nucleocapsid protein protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MSDNGPQSNQRSAPRITFGGPTDSTDNNQNGGRNGARPKQRRPQGLPNNTASWFTALTQHGKEELRFPRGQGVPINTNSGPDDQIGYYRRATRRVRGGDGKMKELSPRWYFYYLGTGPEASLPYGANKEGIVWVATEGALNTPKDHIGTRNPNNNAATVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRGNSRNSTPGSSRGNSPARMASGGGETALALLLLDRLNQLESKVSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKQYNVTQAFGRRGPEQTQGNFGDQDLIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYHGAIKLDDKDPQFKDNVILLNKHIDAYKTFPPTEPKKDKKKKTDEAQPLPQRQKKQPTVTLLPAADMDDFSRQLQNSMSGASADSTQA mint Major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid 43FC8750F1253034 ncap_sars2 Nucleoprotein N Nucleocapsid protein protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA ncap_mers1 Nucleoprotein N Nucleocapsid protein 6 protein protein cvemc Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (Betacoronavirus England 1) Betacoronavirus England 1 Human coronavirus EMC MASPAAPRAVSFADNNDITNTNLSRGRGRNPKPRAAPNNTVSWYTGLTQHGKVPLTFPPGQGVPLNANSTPAQNAGYWRRQDRKINTGNGIKQLAPRWYFYYTGTGPEAALPFRAVKDGIVWVHEDGATDAPSTFGTRNPNNDSAIVTQFAPGTKLPKNFHIEGTGGNSQSSSRASSVSRNSSRSSSQGSRSGNSTRGTSPGPSGIGAVGGDLLYLDLLNRLQALESGKVKQSQPKVITKKDAAAAKNKMRHKRTSTKSFNMVQAFGLRGPGDLQGNFGDLQLNKLGTEDPRWPQIAELAPTASAFMGMSQFKLTHQNNDDHGNPVYFLRYSGAIKLDPKNPNYNKWLELLEQNIDAYKTFPKKEKKQKAPKEESTDQMSEPPKEHRVQGTQRTRTRPSVQPGPMIDVNTD 8AF44237C8308608 g3bp1_human Ras GTPase-activating protein-binding protein 1 G3BP1 G3BP ATP-dependent DNA helicase VIII GAP SH3 domain-binding protein 1 protein protein human Homo sapiens Human MVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPEERQQTPEVVPDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPVLEETAPEDAQKSSSPAPADIAQTVQEDLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPASQPRPESKPESQIPPQRPQRDQRVREQRINIPPQRGPRPIREAGEQGDIEPRRMVRHPDSHQLFIGNLPHEVDKSELKDFFQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRPIMFRGEVRLNVEEKKTRAAREGDRRDNRLRGPGGPRGGLGGGMRGPPRGGMVQKPGFGVGRGLAPRQ 0F9429D78E0C7F59 g3bp1-n-12 2 7 unspecified role unspecified role prey prey purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0DTC9:p.Ile15_Gly18delinsAlaAlaAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain ITFG AAAA Tue Oct 05 19:23:36 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile15_Gly18delinsAlaAlaAlaAla' P0DTC9:p.[Ile15Ala;Phe17Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain F A Tue Oct 05 19:26:11 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile15Ala;Phe17Ala]' P0DTC9:p.Ile15Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I A Tue Oct 05 18:51:04 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile15Ala' P0DTC9:p.Thr16Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T A Tue Oct 05 19:13:47 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr16Ala' P0DTC9:p.Phe17Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F A Tue Oct 05 19:25:56 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe17Ala' P0DTC9:p.Gly18Glu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G E Tue Oct 05 18:27:56 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly18Glu' ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role bait bait purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ntf2 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 1B, Supp. figure 1A All 4 residues within the ITFG motif contribute to the binding, with I and F making the greatest contribution. g3bp1-n-13 3 9 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed P0DTC9:p.Ile15_Gly18delinsAlaAlaAlaAla mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain ITFG AAAA Tue Oct 05 18:48:57 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile15_Gly18delinsAlaAlaAlaAla' P0DTC9:p.[Ile15Ala;Phe17Ala] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain F A Tue Oct 05 18:50:50 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile15Ala;Phe17Ala]' P0DTC9:p.Phe17Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain F A Tue Oct 05 19:19:33 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe17Ala' P0DTC9:p.[Arg92Glu;Arg107Glu;Arg149Glu] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain R E certain certain sequence position certain certain certain sequence position certain R E certain certain sequence position certain certain certain sequence position certain R E Tue Oct 05 18:30:23 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg92Glu;Arg107Glu;Arg149Glu]' region mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 1G Several mutations significantly impaired the inhibitory effect of N protein on G3BP1 phase separation/ stress granule formation. g3bp1-n-14 1 7 unspecified role unspecified role prey prey purified purified n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role bait bait purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ntf2 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. figure 1B All 4 residues within the ITFG motif contribute to the binding, with I and F making the greatest contribution. g3bp1-n-15 4 7 unspecified role unspecified role neutral component neutral component purified purified P0DTC9:p.[Ile15Ala;Thr16Ala;Phe17Ala;Gly18Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain G A Tue Oct 05 18:27:46 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile15Ala;The16Ala;Phe17Ala;Gly18Ala]' P0DTC9:p.Ile15Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I A Tue Oct 05 19:31:43 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile15Ala' P0DTC9:p.Thr16Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T A Tue Oct 05 18:55:21 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.The16Ala' P0DTC9:p.Phe17Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F A Tue Oct 05 19:27:59 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe17Ala' P0DTC9:p.Gly18Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G A Tue Oct 05 18:43:38 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly18Ala' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 9 unspecified role unspecified role neutral component neutral component purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ntf2 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. figure 1 C N11-24 bound to G3BP1-NTF2 with an affinity of ~10 μM, and the affinity was reduced by 6~7-fold for both the I/A and F/A mutations. g3bp1-n-16 2 7 unspecified role unspecified role prey prey purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role bait bait purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ntf2 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q13283:p.Phe15Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F A Tue Oct 05 19:40:30 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe15Ala' Q13283:p.[Phe15Ala;Phe33Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A Tue Oct 05 18:49:25 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe15Ala;Phe33Ala]' Q13283:p.[Phe15Ala;Phe33Ala;Phe124Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A Tue Oct 05 19:26:07 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe15Ala;Phe33Ala;Phe124Ala]' ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. figure 1D g3bp1-n-17 4 7 unspecified role unspecified role prey prey purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 9 unspecified role unspecified role bait bait purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ntf2 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q13283:p.Phe15Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F A Tue Oct 05 19:11:14 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe15Ala' Q13283:p.[Phe15Ala;Phe33Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A Tue Oct 05 18:40:02 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe15Ala;Phe33Ala]' Q13283:p.[Phe15Ala;Phe33Ala;Phe124Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A Tue Oct 05 18:35:30 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe15Ala;Phe33Ala;Phe124Ala]' ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. figure 1E g3bp1-n-18 2 6 unspecified role unspecified role prey prey purified purified possible tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role bait bait purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ntf2 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. figure 1F g3bp1-n-19 2 8 unspecified role unspecified role prey prey purified purified possible tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role bait bait purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ntf2 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. figure 1F very weak interaction g3bp1-n-20 2 7 unspecified role unspecified role prey prey purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role bait bait purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ntf2 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9UN86:p.Phe15Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F A Tue Oct 05 18:40:02 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe15Ala' Q9UN86:p.[Phe15Ala;Phe33Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A Tue Oct 05 19:06:54 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe15Ala;Phe33Ala]' Q9UN86:p.[Phe15Ala;Phe33Ala;Phe124Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A Tue Oct 05 19:26:54 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe15Ala;Phe33Ala;Phe124Ala]' ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. figure 1G No interaction was seen with the N (aas) 1-246 -4A mutant, as well.