DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Masitinib is a broad coronavirus 3CL inhibitor that blocks replication of SARS-CoV-2. Masitinib is a broad coronavirus 3CL inhibitor that blocks replication of SARS-CoV-2. Science (New York, N.Y.) 2021 imex curation Drayman N., DeMarco JK., Jones KA., Azizi SA., Froggatt HM., Tan K., Maltseva NI., Chen S., Nicolaescu V., Dvorkin S., Furlong K., Kathayat RS., Firpo MR., Mastrodomenico V., Bruce EA., Schmidt MM., Jedrzejczak R., Muñoz-Alía MÁ., Schuster B., Nair V., Han KY., O'Brien A., Tomatsidou A., Meyer B., Vignuzzi M., Missiakas D., Botten JW., Brooke CB., Lee H., Baker SC., Mounce BC., Heaton NS., Severson WE., Palmer KE., Dickinson BC., Joachimiak A., Randall G., Tay S. tays@uchicago.edu (S.T.); nirdra@uchicago.edu (N.D.) Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction experimental particp experimental participant identification experimental particp Drayman N., DeMarco JK., Jones KA., Azizi SA., Froggatt HM., Tan K., Maltseva NI., Chen S., Nicolaescu V., Dvorkin S., Furlong K., Kathayat RS., Firpo MR., Mastrodomenico V., Bruce EA., Schmidt MM., Jedrzejczak R., Muñoz-Alía MÁ., Schuster B., Nair V., Han KY., O'Brien A., Tomatsidou A., Meyer B., Vignuzzi M., Missiakas D., Botten JW., Brooke CB., Lee H., Baker SC., Mounce BC., Heaton NS., Severson WE., Palmer KE., Dickinson BC., Joachimiak A., Randall G., Tay S. tays@uchicago.edu (S.T.); nirdra@uchicago.edu (N.D.) Accepted 2021-SEP-30 AT 10:45 BST AT 10:45 BST by ORCHARD Science (New York, N.Y.) 2021 imex curation p0dtd1-pro_0000449623 3C-like proteinase nsp5 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV SGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQ 3264 3569 rep-282 1 2 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma direct interaction direct interaction Fig. 3d, Table S4