IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Repression by the <i>Arabidopsis</i> TOPLESS corepressor requires association with the core mediator complex. Repression by the <i>Arabidopsis</i> TOPLESS corepressor requires association with the core mediator complex. eLife 2021 imex curation Alexander R. Leydon, Wei Wang2, Hardik P. Gala1, Sabrina Gilmour1, Samuel Juarez-Solis, Mollye L. Zahler1, Joseph E. Zemke, Ning Zheng, Jennifer L. Nemhauser, Leydon AR., Wang W., Gala HP., Gilmour S., Juarez-Solis S., Zahler ML., Zemke JE., Zheng N., Nemhauser JL. 2021-05-28: Dr. Alexander R Leydon, University of Washington, Seattle , USA curation material can be found at smb://pcserv.ebi.ac.uk/sp-pro6/intact/local/data/curation-material/2021/alexander-2021 jn7@uw.edu Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Alexander R. Leydon, Wei Wang2, Hardik P. Gala1, Sabrina Gilmour1, Samuel Juarez-Solis, Mollye L. Zahler1, Joseph E. Zemke, Ning Zheng, Jennifer L. Nemhauser 2021-05-28: Dr. Alexander R Leydon, University of Washington, Seattle , USA curation material can be found at smb://pcserv.ebi.ac.uk/sp-pro6/intact/local/data/curation-material/2021/alexander-2021 jn7@uw.edu Accepted 2021-JUN-28 AT 11:26 BST AT 11:26 BST by LPERFETTO Only protein-protein interactions imex curation imex curation Repression by the <i>Arabidopsis</i> TOPLESS corepressor requires association with the core mediator complex. Repression by the <i>Arabidopsis</i> TOPLESS corepressor requires association with the core mediator complex. eLife 2021 imex curation Alexander R. Leydon, Wei Wang2, Hardik P. Gala1, Sabrina Gilmour1, Samuel Juarez-Solis, Mollye L. Zahler1, Joseph E. Zemke, Ning Zheng, Jennifer L. Nemhauser, Leydon AR., Wang W., Gala HP., Gilmour S., Juarez-Solis S., Zahler ML., Zemke JE., Zheng N., Nemhauser JL. 2021-05-28: Dr. Alexander R Leydon, University of Washington, Seattle , USA curation material can be found at smb://pcserv.ebi.ac.uk/sp-pro6/intact/local/data/curation-material/2021/alexander-2021 jn7@uw.edu Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Alexander R. Leydon, Wei Wang2, Hardik P. Gala1, Sabrina Gilmour1, Samuel Juarez-Solis, Mollye L. Zahler1, Joseph E. Zemke, Ning Zheng, Jennifer L. Nemhauser 2021-05-28: Dr. Alexander R Leydon, University of Washington, Seattle , USA curation material can be found at smb://pcserv.ebi.ac.uk/sp-pro6/intact/local/data/curation-material/2021/alexander-2021 jn7@uw.edu Accepted 2021-JUN-28 AT 11:26 BST AT 11:26 BST by LPERFETTO Only protein-protein interactions imex curation imex curation Repression by the <i>Arabidopsis</i> TOPLESS corepressor requires association with the core mediator complex. Repression by the <i>Arabidopsis</i> TOPLESS corepressor requires association with the core mediator complex. eLife 2021 imex curation Alexander R. Leydon, Wei Wang2, Hardik P. Gala1, Sabrina Gilmour1, Samuel Juarez-Solis, Mollye L. Zahler1, Joseph E. Zemke, Ning Zheng, Jennifer L. Nemhauser, Leydon AR., Wang W., Gala HP., Gilmour S., Juarez-Solis S., Zahler ML., Zemke JE., Zheng N., Nemhauser JL. 2021-05-28: Dr. Alexander R Leydon, University of Washington, Seattle , USA curation material can be found at smb://pcserv.ebi.ac.uk/sp-pro6/intact/local/data/curation-material/2021/alexander-2021 jn7@uw.edu Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast ub reconstruction ubiquitin reconstruction ub reconstruction predetermined predetermined participant predetermined Alexander R. Leydon, Wei Wang2, Hardik P. Gala1, Sabrina Gilmour1, Samuel Juarez-Solis, Mollye L. Zahler1, Joseph E. Zemke, Ning Zheng, Jennifer L. Nemhauser 2021-05-28: Dr. Alexander R Leydon, University of Washington, Seattle , USA curation material can be found at smb://pcserv.ebi.ac.uk/sp-pro6/intact/local/data/curation-material/2021/alexander-2021 jn7@uw.edu Accepted 2021-JUN-28 AT 11:26 BST AT 11:26 BST by LPERFETTO Only protein-protein interactions imex curation imex curation Repression by the <i>Arabidopsis</i> TOPLESS corepressor requires association with the core mediator complex. Repression by the <i>Arabidopsis</i> TOPLESS corepressor requires association with the core mediator complex. eLife 2021 imex curation Alexander R. Leydon, Wei Wang2, Hardik P. Gala1, Sabrina Gilmour1, Samuel Juarez-Solis, Mollye L. Zahler1, Joseph E. Zemke, Ning Zheng, Jennifer L. Nemhauser, Leydon AR., Wang W., Gala HP., Gilmour S., Juarez-Solis S., Zahler ML., Zemke JE., Zheng N., Nemhauser JL. 2021-05-28: Dr. Alexander R Leydon, University of Washington, Seattle , USA curation material can be found at smb://pcserv.ebi.ac.uk/sp-pro6/intact/local/data/curation-material/2021/alexander-2021 jn7@uw.edu Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast pull down pull down anti tag western anti tag western blot anti tag western Alexander R. Leydon, Wei Wang2, Hardik P. Gala1, Sabrina Gilmour1, Samuel Juarez-Solis, Mollye L. Zahler1, Joseph E. Zemke, Ning Zheng, Jennifer L. Nemhauser 2021-05-28: Dr. Alexander R Leydon, University of Washington, Seattle , USA curation material can be found at smb://pcserv.ebi.ac.uk/sp-pro6/intact/local/data/curation-material/2021/alexander-2021 jn7@uw.edu Accepted 2021-JUN-28 AT 11:26 BST AT 11:26 BST by LPERFETTO Only protein-protein interactions imex curation imex curation Repression by the <i>Arabidopsis</i> TOPLESS corepressor requires association with the core mediator complex. Repression by the <i>Arabidopsis</i> TOPLESS corepressor requires association with the core mediator complex. eLife 2021 imex curation Alexander R. Leydon, Wei Wang2, Hardik P. Gala1, Sabrina Gilmour1, Samuel Juarez-Solis, Mollye L. Zahler1, Joseph E. Zemke, Ning Zheng, Jennifer L. Nemhauser, Leydon AR., Wang W., Gala HP., Gilmour S., Juarez-Solis S., Zahler ML., Zemke JE., Zheng N., Nemhauser JL. 2021-05-28: Dr. Alexander R Leydon, University of Washington, Seattle , USA curation material can be found at smb://pcserv.ebi.ac.uk/sp-pro6/intact/local/data/curation-material/2021/alexander-2021 jn7@uw.edu Only protein-protein interactions imex curation nicbe Nicotiana benthamiana bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Alexander R. Leydon, Wei Wang2, Hardik P. Gala1, Sabrina Gilmour1, Samuel Juarez-Solis, Mollye L. Zahler1, Joseph E. Zemke, Ning Zheng, Jennifer L. Nemhauser 2021-05-28: Dr. Alexander R Leydon, University of Washington, Seattle , USA curation material can be found at smb://pcserv.ebi.ac.uk/sp-pro6/intact/local/data/curation-material/2021/alexander-2021 jn7@uw.edu Accepted 2021-JUN-28 AT 11:26 BST AT 11:26 BST by LPERFETTO Only protein-protein interactions imex curation imex curation Repression by the <i>Arabidopsis</i> TOPLESS corepressor requires association with the core mediator complex. Repression by the <i>Arabidopsis</i> TOPLESS corepressor requires association with the core mediator complex. eLife 2021 imex curation Alexander R. Leydon, Wei Wang2, Hardik P. Gala1, Sabrina Gilmour1, Samuel Juarez-Solis, Mollye L. Zahler1, Joseph E. Zemke, Ning Zheng, Jennifer L. Nemhauser, Leydon AR., Wang W., Gala HP., Gilmour S., Juarez-Solis S., Zahler ML., Zemke JE., Zheng N., Nemhauser JL. 2021-05-28: Dr. Alexander R Leydon, University of Washington, Seattle , USA curation material can be found at smb://pcserv.ebi.ac.uk/sp-pro6/intact/local/data/curation-material/2021/alexander-2021 jn7@uw.edu Only protein-protein interactions imex curation nicbe-leaf Nicotiana benthamiana leaf leaf leaf anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Alexander R. Leydon, Wei Wang2, Hardik P. Gala1, Sabrina Gilmour1, Samuel Juarez-Solis, Mollye L. Zahler1, Joseph E. Zemke, Ning Zheng, Jennifer L. Nemhauser 2021-05-28: Dr. Alexander R Leydon, University of Washington, Seattle , USA curation material can be found at smb://pcserv.ebi.ac.uk/sp-pro6/intact/local/data/curation-material/2021/alexander-2021 jn7@uw.edu Accepted 2021-JUN-28 AT 11:26 BST AT 11:26 BST by LPERFETTO Only protein-protein interactions imex curation imex curation med21_arath Mediator of RNA polymerase II transcription subunit 21 Mediator complex subunit 21 RNAPII complex component SRB7 MED21 MED21_1 SRB7 T4B21.7 At4g04780 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDIISQLQEQVNTIAAITFNAFGTLQRDAPPVQLSPNYPEPPATTTVTDDATPFPEQPKQLSAGLVKAAKQFDALVAALPLSEGGEGAQLKRIAELQVENDLVGQELQKQLEAAEKELKQVQELFGQAADNCLNMKKPE iaa3_arath Auxin-responsive protein IAA3 IAA3 SHY2 At1g04240 F19P19.32 Indoleacetic acid-induced protein 3 Short hypocotyl Suppressor of HY2 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDEFVNLKETELRLGLPGTDNVCEAKERVSCCNNNNKRVLSTDTEKEIESSSRKTETSPPRKAQIVGWPPVRSYRKNNIQSKKNESEHEGQGIYVKVSMDGAPYLRKIDLSCYKGYSELLKALEVMFKFSVGEYFERDGYKGSDFVPTYEDKDGDWMLIGDVPWEMFICTCKRLRIMKGSEAKGLGCGV DIP protein Q38822 original sequence version: 82 DIP protein Q38822 original sequence version: 104 8627A4173363EF27 tpl_arath Protein TOPLESS TPL WSIP1 WUS-interacting protein 1 F7H2.9 At1g15750 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSSLSRELVFLILQFLDEEKFKETVHKLEQESGFFFNMKYFEDEVHNGNWDEVEKYLSGFTKVDDNRYSMKIFFEIRKQKYLEALDKHDRPKAVDILVKDLKVFSTFNEELFKEITQLLTLENFRENEQLSKYGDTKSARAIMLVELKKLIEANPLFRDKLQFPTLRNSRLRTLINQSLNWQHQLCKNPRPNPDIKTLFVDHSCGPPNGARAPSPVNNPLLGGIPKAGGFPPLGAHGPFQPTASPVPTPLAGWMSSPSSVPHPAVSAGAIALGGPSIPAALKHPRTPPTNASLDYPSADSEHVSKRTRPMGISDEVNLGVNMLPMSFSGQAHGHSPAFKAPDDLPKTVARTLSQGSSPMSMDFHPIKQTLLLVGTNVGDIGLWEVGSRERLVQKTFKVWDLSKCSMPLQAALVKEPVVSVNRVIWSPDGSLFGVAYSRHIVQLYSYHGGEDMRQHLEIDAHVGGVNDISFSTPNKQLCVITCGDDKTIKVWDAATGVKRHTFEGHEAPVYSVCPHYKENIQFIFSTALDGKIKAWLYDNMGSRVDYDAPGRWCTTMAYSADGTRLFSCGTSKDGESFIVEWNESEGAVKRTYQGFHKRSLGVVQFDTTKNRYLAAGDDFSIKFWDMDAVQLLTAIDGDGGLQASPRIRFNKEGSLLAVSGNENVIKIMANSDGLRLLHTFENISSESSKPAINSIAAAAAAAATSAGHADRSANVVSIQGMNGDSRNMVDVKPVITEESNDKSKIWKLTEVSEPSQCRSLRLPENLRVAKISRLIFTNSGNAILALASNAIHLLWKWQRNERNATGKATASLPPQQWQPASGILMTNDVAETNPEEAVPCFALSKNDSYVMSASGGKISLFNMMTFKTMATFMPPPPAATFLAFHPQDNNIIAIGMDDSTIQIYNVRVDEVKSKLKGHSKRITGLAFSNVLNVLVSSGADAQLCVWNTDGWEKQRSKVLPLPQGRPNSAPSDTRVQFHQDQAHFLVVHETQLAIYETTKLECMKQWAVRESLAPITHATFSCDSQLVYASFMDATVCVFSSANLRLRCRVNPSAYLPASLSNSNVHPLVIAAHPQEPNMFAVGLSDGGVHIFEPLESEGKWGVAPPAENGSASGAPTAPSVGASASDQPQR DIP protein Q94AI7 original sequence version: 64 6211EE51BC309299 iaa7_arath Auxin-responsive protein IAA7 Indoleacetic acid-induced protein 7 Auxin resistant 2 IAA7 AXR2 At3g23050 MXC7.8 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MIGQLMNLKATELCLGLPGGAEAVESPAKSAVGSKRGFSETVDLMLNLQSNKEGSVDLKNVSAVPKEKTTLKDPSKPPAKAQVVGWPPVRNYRKNMMTQQKTSSGAEEASSEKAGNFGGGAAGAGLVKVSMDGAPYLRKVDLKMYKSYQDLSDALAKMFSSFTMGNYGAQGMIDFMNESKLMNLLNSSEYVPSYEDKDGDWMLVGDVPWEMFVESCKRLRIMKGSEAVGLAPRAMEKYCKNRS DIP protein Q38825 original sequence version: 111 DIP protein Q38825 original sequence version: 70 DIP protein Q38825 original sequence version: 88 830E9B9EA2C2B5D0 md10b_arath Mediator of RNA polymerase II transcription subunit 10b MED10B MED10_1 T24P13.4 At1g26665 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDPTQNTSAGIGGSNGTIRYQTNDGTSTVTVADDSKENLSQVINSIEKTLGVLHQLHLTVTSFTPASQLHLLQRLNSLVMELDNMTKLSEKCNIQIPMEVLNLIDDGKNPDEFTKDVLNSCIARNQVTKGKTDAFKDLRKHILEELEQNFPDEVDMYREIRASSAAVTKRLAQSQSVLPNGDAKVKNEL C654B120A63A0FAC med21-tpl-1 3 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n-terminus ubiquitin reconstruction tag ubiquitin reconstruction tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed Q94AI7:p.Val145Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain V A Q94AI7:p.Glu146Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E A Q94AI7:p.Lys148Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K A Q94AI7:p.Lys149Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K A Q94AI7:p.[Val145_Glu146delinsAlaAla;Lys148_Lys149delinsAlaAla] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain VE AA certain certain sequence position certain certain certain sequence position certain KK AA N-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminus ubiquitin reconstruction tag ubiquitin reconstruction tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 2a, 2e, 3d, Figure 2 - Figure Supplement 1b med10b-tpl-1 3 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n-terminus ubiquitin reconstruction tag ubiquitin reconstruction tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminus ubiquitin reconstruction tag ubiquitin reconstruction tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q94AI7:p.[Val145_Glu146delinsAlaAla;Lys148_Lys149delinsAlaAla] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain VE AA certain certain sequence position certain certain certain sequence position certain KK AA physical association physical association 2c, 2d-e, Figure 2 - Figure Supplement 1d med21-tpl-2 4 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tag lexa dna bd lexa dna binding domain lexa dna bd undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined tag vp16 ad vp16 activation domain vp16 ad undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined tag protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2d med10b-tpl-2 4 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed tag vp16 ad vp16 activation domain vp16 ad undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined tag protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined tag lexa dna bd lexa dna binding domain lexa dna bd undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined N-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2d med21-tpl-3 5 7 predetermined predetermined participant predetermined tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell c-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range binding region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 9 predetermined predetermined participant predetermined tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q94AI7:p.[Val145_Glu146delinsAlaAla;Lys148_Lys149delinsAlaAla] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain VE AA certain certain sequence position certain certain certain sequence position certain KK AA physical association physical association 6a tpl-tpl-2 5 9 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell c-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 predetermined predetermined participant predetermined tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 6a med21-tpl-4 6 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 6b iaa3-tpl-1 3 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range c-terminus ubiquitin reconstruction tag ubiquitin reconstruction tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n-terminus ubiquitin reconstruction tag ubiquitin reconstruction tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 2a, 2e, Figure 2 - Figure Supplement 1b-c tpl-tpl-3 3 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n-terminus ubiquitin reconstruction tag ubiquitin reconstruction tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range N-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed LisH domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain c-terminus ubiquitin reconstruction tag ubiquitin reconstruction tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range H1-H9 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q94AI7:p.[Val145_Glu146delinsAlaAla;Lys148_Lys149delinsAlaAla] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain VE AA certain certain sequence position certain certain certain sequence position certain KK AA Q94AI7:p.Val145Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain V A Q94AI7:p.Glu146Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A Q94AI7:p.Lys148Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Q94AI7:p.Lys149Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A physical association physical association 2a, 2c-d, 3d, Figure 2 - Figure Supplement 1a-d tpl-1 1 9 unspecified role unspecified role neutral component neutral component purified purified tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined N-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 5h tpl-4 1 9 unspecified role unspecified role neutral component neutral component purified purified Q94AI7:p.[Lys102Ser;Thr116_Gln117delinsAlaSer;Glu122Ser] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K S certain certain sequence position certain certain certain sequence position certain TQ AS certain certain sequence position certain certain certain sequence position certain E S tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined N-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q94AI7:p.[Ser5Ala;Leu8Ser;Phe15Tyr;Glu19Ser;Lys102Ser;Thr116_Gln117delinsAlaSer;Glu122Ser] monomer mutation mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain L S certain certain sequence position certain certain certain sequence position certain F Y certain certain sequence position certain certain certain sequence position certain E S certain certain sequence position certain certain certain sequence position certain K S certain certain sequence position certain certain certain sequence position certain TQ AS certain certain sequence position certain certain certain sequence position certain E S ecoli Escherichia coli (strain K12) direct interaction direct interaction 5h tpl-tpl-4 3 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed Q94AI7:p.[Ser5Ala;Leu8Ser;Phe15Tyr;Glu19Ser;Lys102Ser;Thr116_Gln117delinsAlaSer;Glu122Ser] monomer mutation mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain L S certain certain sequence position certain certain certain sequence position certain F Y certain certain sequence position certain certain certain sequence position certain E S certain certain sequence position certain certain certain sequence position certain K S certain certain sequence position certain certain certain sequence position certain TQ AS certain certain sequence position certain certain certain sequence position certain E S c-terminus ubiquitin reconstruction tag ubiquitin reconstruction tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range N-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed Q94AI7:p.[Ser5Ala;Leu8Ser;Phe15Tyr;Glu19Ser;Lys102Ser;Thr116_Gln117delinsAlaSer;Glu122Ser] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain L S certain certain sequence position certain certain certain sequence position certain F Y certain certain sequence position certain certain certain sequence position certain E S certain certain sequence position certain certain certain sequence position certain K S certain certain sequence position certain certain certain sequence position certain TQ AS certain certain sequence position certain certain certain sequence position certain E S tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n-terminus ubiquitin reconstruction tag ubiquitin reconstruction tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range N-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q94AI7:p.[Lys102Ser;Thr116_Gln117delinsAlaSer;Glu122Ser] LDimer mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K S certain certain sequence position certain certain certain sequence position certain TQ AS certain certain sequence position certain certain certain sequence position certain E S physical association physical association 5I-1 TPL Monomer bait and TPL LDimer, monomer prey tpl-tpl-5 3 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n-terminus ubiquitin reconstruction tag ubiquitin reconstruction tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range N-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed Q94AI7:p.[Ser5Ala;Leu8Ser;Phe15Tyr;Glu19Ser;Lys102Ser;Thr116_Gln117delinsAlaSer;Glu122Ser] Monomer mutations mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K S certain certain sequence position certain certain certain sequence position certain TQ AS certain certain sequence position certain certain certain sequence position certain E S certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain F Y certain certain sequence position certain certain certain sequence position certain E S certain certain sequence position certain certain certain sequence position certain L S c-terminus ubiquitin reconstruction tag ubiquitin reconstruction tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range N-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 5I-2 TPL monomer bait and TPL prey tpl-tpl-6 2 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N-terminal region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 2 - Figure Supplement 1. tpl-iaa7-1 2 10 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 2 - Figure Supplement 1a tpl-med21-1 2 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain N-terminal region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 2 - Figure Supplement 1a tpl-med21-2 3 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n-terminus ubiquitin reconstruction tag ubiquitin reconstruction tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range N-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed H1-H9 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain H1-H7 domain required to bind necessary binding region required to bind certain certain sequence position certain range range c-terminus ubiquitin reconstruction tag ubiquitin reconstruction tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Figure 2 - Figure Supplement 1c med10b-med21-1 6 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Figure 6 - Figure Supplement 1 tpl-tpl-7 3 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n-terminus ubiquitin reconstruction tag ubiquitin reconstruction tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range N-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminus ubiquitin reconstruction tag ubiquitin reconstruction tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 5I-3 tpl-tpl-8 4 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tag lexa dna bd lexa dna binding domain lexa dna bd undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined tag vp16 ad vp16 activation domain vp16 ad undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined tag protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2d