IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Two conserved oligomer interfaces of NSP7 and NSP8 underpin the dynamic assembly of SARS-CoV-2 RdRP. Two conserved oligomer interfaces of NSP7 and NSP8 underpin the dynamic assembly of SARS-CoV-2 RdRP. Nucleic acids research 2021 imex curation Biswal M., Diggs S., Xu D., Khudaverdyan N., Lu J., Fang J., Blaha G., Hai R., Song J. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus jikui.song@ucr.edu,ronghai@ucr.edu Only protein-protein interactions imex curation in vitro In vitro crosslink cross-linking study crosslink weight by staining molecular weight estimation by staining weight by staining Biswal M., Diggs S., Xu D., Khudaverdyan N., Lu J., Fang J., Blaha G., Hai R., Song J. Virus - Publications including interactions involving viral proteins Figure 3 + more; I have mostly taken the effect of mutations from the text as I do not see several of their stated effects. (+ seen some they do not mention). Interactions have been entered as shown experimentally- separated the "sufficient to Bind" results from mutations. Cross linker EGS:ethylene glycol bis(succinimidyl succinate) jikui.song@ucr.edu,ronghai@ucr.edu Accepted 2021-JUN-21 AT 08:10 BST AT 08:10 BST by ORCHARD Nucleic acids research 2021 Only protein-protein interactions imex curation imex curation Two conserved oligomer interfaces of NSP7 and NSP8 underpin the dynamic assembly of SARS-CoV-2 RdRP. Two conserved oligomer interfaces of NSP7 and NSP8 underpin the dynamic assembly of SARS-CoV-2 RdRP. Nucleic acids research 2021 imex curation Biswal M., Diggs S., Xu D., Khudaverdyan N., Lu J., Fang J., Blaha G., Hai R., Song J. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus jikui.song@ucr.edu,ronghai@ucr.edu Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Biswal M., Diggs S., Xu D., Khudaverdyan N., Lu J., Fang J., Blaha G., Hai R., Song J. Virus - Publications including interactions involving viral proteins I have left all mutations as "decreasing" as I cannot tell the difference between 'decreasing' and 'disrupting' by looking at Gel filtration figures. (one lump or two - or three?). Interactions have been entered as shown experimentally- separated the "sufficient to Bind" results from mutations. jikui.song@ucr.edu,ronghai@ucr.edu Accepted 2021-JUN-21 AT 08:10 BST AT 08:10 BST by ORCHARD Nucleic acids research 2021 Only protein-protein interactions imex curation imex curation Two conserved oligomer interfaces of NSP7 and NSP8 underpin the dynamic assembly of SARS-CoV-2 RdRP. Two conserved oligomer interfaces of NSP7 and NSP8 underpin the dynamic assembly of SARS-CoV-2 RdRP. Nucleic acids research 2021 imex curation Biswal M., Diggs S., Xu D., Khudaverdyan N., Lu J., Fang J., Blaha G., Hai R., Song J. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus jikui.song@ucr.edu,ronghai@ucr.edu Only protein-protein interactions imex curation in vitro In vitro rna rna pol assay rna directed rna polymerase assay rna rna pol assay tag fluorescence tag visualisation by fluorescence tag fluorescence Biswal M., Diggs S., Xu D., Khudaverdyan N., Lu J., Fang J., Blaha G., Hai R., Song J. Virus - Publications including interactions involving viral proteins I cannot compare NSP12 native and NC_045512 sequences as no sequence or reference appears to be given for bacterially optimised version. jikui.song@ucr.edu,ronghai@ucr.edu Accepted 2021-JUN-21 AT 08:10 BST AT 08:10 BST by ORCHARD Nucleic acids research 2021 Only protein-protein interactions imex curation imex curation Two conserved oligomer interfaces of NSP7 and NSP8 underpin the dynamic assembly of SARS-CoV-2 RdRP. Two conserved oligomer interfaces of NSP7 and NSP8 underpin the dynamic assembly of SARS-CoV-2 RdRP. Nucleic acids research 2021 imex curation Biswal M., Diggs S., Xu D., Khudaverdyan N., Lu J., Fang J., Blaha G., Hai R., Song J. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus jikui.song@ucr.edu,ronghai@ucr.edu Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Biswal M., Diggs S., Xu D., Khudaverdyan N., Lu J., Fang J., Blaha G., Hai R., Song J. Virus - Publications including interactions involving viral proteins jikui.song@ucr.edu,ronghai@ucr.edu Accepted 2021-JUN-21 AT 08:10 BST AT 08:10 BST by ORCHARD Nucleic acids research 2021 Only protein-protein interactions imex curation imex curation p0dtd1-pro_0000449625 Non-structural protein 7 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV SKMSDVKCTSVVLLSVLQQLRVESSSKLWAQCVQLHNDILLAKDTTEAFEKMVSLLSVLLSMQGAVDINKLCEEMLDNRATLQ 3860 3942 p0dtd1-pro_0000449629 RNA-directed RNA polymerase nsp12 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV SADAQSFLNRVCGVSAARLTPCGTGTSTDVVYRAFDIYNDKVAGFAKFLKTNCCRFQEKDEDDNLIDSYFVVKRHTFSNYQHEETIYNLLKDCPAVAKHDFFKFRIDGDMVPHISRQRLTKYTMADLVYALRHFDEGNCDTLKEILVTYNCCDDDYFNKKDWYDFVENPDILRVYANLGERVRQALLKTVQFCDAMRNAGIVGVLTLDNQDLNGNWYDFGDFIQTTPGSGVPVVDSYYSLLMPILTLTRALTAESHVDTDLTKPYIKWDLLKYDFTEERLKLFDRYFKYWDQTYHPNCVNCLDDRCILHCANFNVLFSTVFPPTSFGPLVRKIFVDGVPFVVSTGYHFRELGVVHNQDVNLHSSRLSFKELLVYAADPAMHAASGNLLLDKRTTCFSVAALTNNVAFQTVKPGNFNKDFYDFAVSKGFFKEGSSVELKHFFFAQDGNAAISDYDYYRYNLPTMCDIRQLLFVVEVVDKYFDCYDGGCINANQVIVNNLDKSAGFPFNKWGKARLYYDSMSYEDQDALFAYTKRNVIPTITQMNLKYAISAKNRARTVAGVSICSTMTNRQFHQKLLKSIAATRGATVVIGTSKFYGGWHNMLKTVYSDVENPHLMGWDYPKCDRAMPNMLRIMASLVLARKHTTCCSLSHRFYRLANECAQVLSEMVMCGGSLYVKPGGTSSGDATTAYANSVFNICQAVTANVNALLSTDGNKIADKYVRNLQHRLYECLYRNRDVDTDFVNEFYAYLRKHFSMMILSDDAVVCFNSTYASQGLVASIKNFKSVLYYQNNVFMSEAKCWTETDLTKGPHEFCSQHTMLVKQGDDYVYLPYPDPSRILGAGCFVDDIVKTDGTLMIERFVSLAIDAYPLTKHPNQEYADVFHLYLQYIRKLHDELTGHMLDMYSVMLTNDNTSRYWEPEFYEAMYTPHTVLQ 4393 5324 p0dtd1-pro_0000449626 Non-structural protein 8 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV AIASEFSSLPSYAAFATAQEAYEQAVANGDSEVVLKKLKKSLNVAKSEFDRDAAMQRKLEKMADQAMTQMYKQARSEDKRAKVTSAMQTMLFTMLRKLDNDALNNIINNARDGCVPLNIIPLTTAAKLMVVIPDYNTYKNTCDGTTFTYASALWEIQQVVDADSKIVQLSEISMDNSPNLAWPLIVTALRANSAVKLQ 3943 4140 rna_substrate ds rna ds rna double stranded ribonucleic acid chemical synthesis Chemical synthesis (Chemical synthesis) UUUUCAUGCUACGCGUAGUUUUCUACGCG rep-rep-196 4 7 unspecified role unspecified role neutral component neutral component purified purified 5 unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction Table S1 rep-rep-197 2 7 unspecified role unspecified role neutral component neutral component purified purified C-terminal region/delta73 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction 1B, Supp. figure 4E The NSP7-NSP8 mixture eluted at a volume corresponding to what is expected for a 2:2 tetrameric form (∼62 kDa). I have NOT added a dimer for NSP8 as the fig. 1b does not show this (according to 1b the NSP8 dimer looks to have a higher m.wt than the NSP7/8 tetramer, and NSP7 M.Wt is nowhere near 7kDa wt for a monomer.) rep-rep-198 1 7 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component purified purified p.Phe49Ala mutation with complex effect mutation with complex effect certain certain sequence position certain certain certain sequence position certain F A p.Leu56Ala mutation with complex effect mutation with complex effect certain certain sequence position certain certain certain sequence position certain L A p.Met52Ala mutation with complex effect mutation with complex effect certain certain sequence position certain certain certain sequence position certain M A p.Leu71Ala mutation with complex effect mutation with complex effect certain certain sequence position certain certain certain sequence position certain L A P0DTD1-PRO_0000449625:p.Asn37Val mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain N V p.Cys8Gly mutation with complex effect mutation with complex effect certain certain sequence position certain certain certain sequence position certain C G p.Val11Ala mutation with complex effect mutation with complex effect certain certain sequence position certain certain certain sequence position certain V A ecoli Escherichia coli (strain K12) direct interaction direct interaction 1C, 3 C/E, supp. figure 1C/7A NSP7+8 tetramer Mutations of NSP7 F49A and NSP7 L56A (in Figure 3C) and of NSP8 F92A (in Figure 3D) lead to an increased NSP8 dimerization, accompanied by reduction of the hetero-tetramerization of NSP7–NSP8. Mutations of NSP7, C8G and V11A, lead to an even more pronounced increase of NSP8 homodimer at the expense of NSP7-NSP8 heterotetramer. The NSP7 M52A mutation does not appreciably affect the population of NSP8 dimer, but leads to a substantial heterotetramer-to-heterodimer shift of NSP7-NSP8. The NSP7 L71A mutant increases tetramerization of NSP7 with NSP8. Figure S1C appears to show a decrease in NSP8 dimerization and a slight increase in NSP7+8 dimerization. rep-rep-199 1 7 unspecified role unspecified role neutral component neutral component purified purified P0DTD1-PRO_0000449626:p.Met90Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain M A Mon Jun 28 17:39:09 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met90Ala' P0DTD1-PRO_0000449626:p.Phe92Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain F A Mon Jun 28 17:29:28 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe92Ala' P0DTD1-PRO_0000449626:p.Met94Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain M A Mon Jun 28 17:36:51 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met94Ala' ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction 1C, 3F NSP7+8 dimer Reduced band for the M94A mutation not mentioned in text, but (to me) fairly visible. rep-rep-200 1 7 unspecified role unspecified role neutral component neutral component purified purified P0DTD1-PRO_0000449626:p.Phe92Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain F A Mon Jun 28 17:14:35 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe92Ala' P0DTD1-PRO_0000449626:p.Met90Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain M A Mon Jun 28 17:46:59 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met90Ala' P0DTD1-PRO_0000449626:p.Met94Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain M A Mon Jun 28 17:02:41 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met94Ala' ecoli Escherichia coli (strain K12) direct interaction direct interaction 1C, 3 D/F NSP8 dimer rep-201 1 7 unspecified role unspecified role neutral component neutral component purified purified P0DTD1-PRO_0000449626:p.Met90Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain M A Mon Jun 28 16:53:29 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met90Ala' P0DTD1-PRO_0000449626:p.Met94Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain M A Mon Jun 28 17:34:14 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met94Ala' ecoli Escherichia coli (strain K12) direct interaction direct interaction 1C, 3F NSP8 tetramer Met 90/94 not mentioned in text but band looks darker than in fig. 3E for NSP8 tetramer - which IS mentioned. rep-rep-201 1 7 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component purified purified P0DTD1-PRO_0000449625:p.Met52Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain M A Mon Jun 28 17:25:58 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met52Ala' P0DTD1-PRO_0000449625:p.Asn37Val mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain N V Mon Jun 28 17:34:21 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn37Val' P0DTD1-PRO_0000449625:p.Phe49Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain F A Mon Jun 28 17:31:52 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe49Ala' P0DTD1-PRO_0000449625:p.Leu56Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L A Mon Jun 28 17:23:07 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu56Ala' P0DTD1-PRO_0000449625:p.Cys8Gly mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain C G Mon Jun 28 17:09:43 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys8Gly' P0DTD1-PRO_0000449625:p.Val11Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain V A Mon Jun 28 16:47:50 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val11Ala' ecoli Escherichia coli (strain K12) direct interaction direct interaction 1C, 3 C/E, Supp. figure 7A NSP7+8 dimer rep-rep-202 1 7 unspecified role unspecified role neutral component neutral component purified purified p.Phe92Ala mutation with complex effect mutation with complex effect certain certain sequence position certain certain certain sequence position certain F A p.Met90Ala mutation with complex effect mutation with complex effect certain certain sequence position certain certain certain sequence position certain M A p.Met94Ala mutation with complex effect mutation with complex effect certain certain sequence position certain certain certain sequence position certain M A ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction 1C, 3 D/F NSP7+8 tetramer Mutations of NSP7 F49A and NSP7 L56A (in Figure 3C) and of NSP8 F92A (in Figure 3D) lead to an increased NSP8 dimerization, accompanied by reduction of the hetero-tetramerization of NSP7–NSP8. Mutations M90A and M94A, lead to an increased NSP8 dimer formation at the expense of the NSP7–NSP8 heterotetramer. rep-rep-203 3 8 enzyme target enzyme target substrate substrate neutral component neutral component 5' FAM fluorescein fluorescein label fluorescein certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 5 stimulator stimulator ancillary ancillary purified purified P0DTD1-PRO_0000449625:p.[Phe49Ala;Met52Ala;Leu56Ala] mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain M A certain certain sequence position certain certain certain sequence position certain L A Mon Jun 28 17:08:15 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe49Ala;Met52Ala;Leu56Ala]' P0DTD1-PRO_0000449625:p.Cys8Gly mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain C G Mon Jun 28 17:14:02 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys8Gly' P0DTD1-PRO_0000449625:p.Phe49Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain F A Mon Jun 28 17:38:34 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe49Ala' P0DTD1-PRO_0000449625:p.Met52Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain M A Mon Jun 28 16:52:42 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met52Ala' P0DTD1-PRO_0000449625:p.Leu56Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain L A Mon Jun 28 17:17:04 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu56Ala' P0DTD1-PRO_0000449625:p.Val11Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain V A Mon Jun 28 17:02:03 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Vall11Ala' P0DTD1-PRO_0000449625:p.Asn37Val mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain N V Mon Jun 28 17:12:41 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn37Val' ecoli Escherichia coli (strain K12) 7 stimulator stimulator ancillary ancillary purified purified ecoli Escherichia coli (strain K12) 6 enzyme enzyme neutral component neutral component purified purified ecoli Escherichia coli (strain K12) rna elongation rna strand elongation rna elongation 5, (Supp. figure 8B) rna sequence taken from methods and pmid:32438371 Similar results were obtained using WT bacterially optimised NSP12 and "native" NSP12 with sequence from RefSeq identifier NC_045512. rep-rep-204 3 8 enzyme target enzyme target substrate substrate neutral component neutral component 5' FAM fluorescein fluorescein label fluorescein certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 5 stimulator stimulator ancillary ancillary purified purified ecoli Escherichia coli (strain K12) 7 stimulator stimulator ancillary ancillary purified purified P0DTD1-PRO_0000449626:p.Phe92Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain F A Mon Jun 28 16:55:55 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe92Ala' P0DTD1-PRO_0000449626:p.Met90Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain M A Mon Jun 28 17:01:55 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met90Ala' P0DTD1-PRO_0000449626:p.Met94Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain M A Mon Jun 28 17:15:50 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met94Ala' P0DTD1-PRO_0000449626:p.Lys58Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain K A Mon Jun 28 17:25:40 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys58Ala' P0DTD1-PRO_0000449626:p.Arg75Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R A Mon Jun 28 17:16:18 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg75Ala' P0DTD1-PRO_0000449626:p.Lys82Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain K A Mon Jun 28 17:12:28 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg82Ala' ecoli Escherichia coli (strain K12) 6 enzyme enzyme neutral component neutral component purified purified ecoli Escherichia coli (strain K12) rna elongation rna strand elongation rna elongation 5 rna sequence taken from methods and pmid:32438371 Similar results were obtained using WT bacterially optimised NSP12 and "native" NSP12 with sequence from RefSeq identifier NC_045512. rep-rep-205 2 7 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component purified purified P0DTD1-PRO_0000449625:p.Phe49Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F A Mon Jun 28 17:21:14 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe49Ala' P0DTD1-PRO_0000449625:p.Leu56Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L A Mon Jun 28 17:16:29 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu56Ala' P0DTD1-PRO_0000449625:p.Met52Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain M A Mon Jun 28 17:46:55 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met52Ala' P0DTD1-PRO_0000449625:p.Cys8Gly mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain C G Mon Jun 28 17:30:27 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys8Gly' P0DTD1-PRO_0000449625:p.Val11Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain V A Mon Jun 28 17:05:09 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val11Ala' P0DTD1-PRO_0000449625:p.Asn37Val mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain N V ecoli Escherichia coli (strain K12) direct interaction direct interaction supp. figure 3/7B NSP7+8 tetramer;mutations on the interface II of NSP7 lead to even more severe loss of the tetrameric NSP7–NSP8 fraction than mutations on interface I. rep-rep-206 2 7 unspecified role unspecified role neutral component neutral component purified purified P0DTD1-PRO_0000449626:p.Met90Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain M A Mon Jun 28 17:00:04 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met90Ala' P0DTD1-PRO_0000449626:p.Phe92Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F A Mon Jun 28 17:20:44 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe92Ala' P0DTD1-PRO_0000449626:p.Met94Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain M A Mon Jun 28 17:04:40 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met94Ala' ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. figure 3 NSP7+8 tetramer. rep-rep-207 1 7 unspecified role unspecified role neutral component neutral component purified purified C-terminal region/delta73 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. figure 4F NSP7+8 tetramer rep-rep-208 1 7 unspecified role unspecified role neutral component neutral component purified purified C-terminal region/delta73 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction supp. figure 4F NSP7+8 dimer rep-209 1 7 unspecified role unspecified role neutral component neutral component purified purified C-terminal region/delta73 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. figure 4f NSP8 dimer rep-rep-209 2 7 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus sumo tag sumo tag SUMO tag sumo tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus sumo tag sumo tag SUMO tag sumo tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus sumo tag sumo tag SUMO tag sumo tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Supp. figure 7 C/D It is stated that the NSP7N37V mutation leads to a modest, but notable, disruption of the NSP7-NSP8-NSP12 complex. Only added a comment for the mutation, as I see NO detectable difference between figures S7C (WT) and S7D (mutant.)