IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein. GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein. Nature communications 2021 imex curation Zhao M., Yu Y., Sun LM., Xing JQ., Li T., Zhu Y., Wang M., Yu Y., Xue W., Xia T., Cai H., Han QY., Yin X., Li WH., Li AL., Cui J., Yuan Z., Zhang R., Zhou T., Zhang XM., Li T. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus It is not known if the Viral RNA sequences obtained from TSINGKE Biotech were In Vitro or chemically synthesised as the TSINGKE Biotechnology web site is in Chinese only. I have deleted the Light microscopy experiment as I agree that the results look dubious. Supp. figure 4J:I have added the 20-50 vRNAs as separate interactors, as if I added them as a "Sufficient to bind" as a region on the 60nt vRNA, it would not be clear that the 60nt molecule was used for all the other figures. Only protein-protein interactions imex curation in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Zhao M., Yu Y., Sun LM., Xing JQ., Li T., Zhu Y., Wang M., Yu Y., Xue W., Xia T., Cai H., Han QY., Yin X., Li WH., Li AL., Cui J., Yuan Z., Zhang R., Zhou T., Zhang XM., Li T. Virus - Publications including interactions involving viral proteins It is not known if the Viral RNA sequences obtained from TSINGKE Biotech were In Vitro or chemically synthesised as the TSINGKE Biotechnology web site is in Chinese only. I have deleted the Light microscopy experiment as I agree that the results look dubious. Accepted 2021-AUG-24 AT 11:03 BST AT 11:03 BST by LUANA Nature communications 2021 Only protein-protein interactions imex curation imex curation GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein. GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein. Nature communications 2021 imex curation Zhao M., Yu Y., Sun LM., Xing JQ., Li T., Zhu Y., Wang M., Yu Y., Xue W., Xia T., Cai H., Han QY., Yin X., Li WH., Li AL., Cui J., Yuan Z., Zhang R., Zhou T., Zhang XM., Li T. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus It is not known if the Viral RNA sequences obtained from TSINGKE Biotech were In Vitro or chemically synthesised as the TSINGKE Biotechnology web site is in Chinese only. I have deleted the Light microscopy experiment as I agree that the results look dubious. Supp. figure 4J:I have added the 20-50 vRNAs as separate interactors, as if I added them as a "Sufficient to bind" as a region on the 60nt vRNA, it would not be clear that the 60nt molecule was used for all the other figures. Only protein-protein interactions imex curation human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. light microscopy light microscopy predetermined predetermined participant predetermined Zhao M., Yu Y., Sun LM., Xing JQ., Li T., Zhu Y., Wang M., Yu Y., Xue W., Xia T., Cai H., Han QY., Yin X., Li WH., Li AL., Cui J., Yuan Z., Zhang R., Zhou T., Zhang XM., Li T. Virus - Publications including interactions involving viral proteins It is not known if the Viral RNA sequences obtained from TSINGKE Biotech were In Vitro or chemically synthesised as the TSINGKE Biotechnology web site is in Chinese only. I have deleted the Light microscopy experiment as I agree that the results look dubious. Accepted 2021-AUG-24 AT 11:03 BST AT 11:03 BST by LUANA Nature communications 2021 Only protein-protein interactions imex curation imex curation GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein. GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein. Nature communications 2021 imex curation Zhao M., Yu Y., Sun LM., Xing JQ., Li T., Zhu Y., Wang M., Yu Y., Xue W., Xia T., Cai H., Han QY., Yin X., Li WH., Li AL., Cui J., Yuan Z., Zhang R., Zhou T., Zhang XM., Li T. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus It is not known if the Viral RNA sequences obtained from TSINGKE Biotech were In Vitro or chemically synthesised as the TSINGKE Biotechnology web site is in Chinese only. I have deleted the Light microscopy experiment as I agree that the results look dubious. Supp. figure 4J:I have added the 20-50 vRNAs as separate interactors, as if I added them as a "Sufficient to bind" as a region on the 60nt vRNA, it would not be clear that the 60nt molecule was used for all the other figures. Only protein-protein interactions imex curation human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Zhao M., Yu Y., Sun LM., Xing JQ., Li T., Zhu Y., Wang M., Yu Y., Xue W., Xia T., Cai H., Han QY., Yin X., Li WH., Li AL., Cui J., Yuan Z., Zhang R., Zhou T., Zhang XM., Li T. Virus - Publications including interactions involving viral proteins It is not known if the Viral RNA sequences obtained from TSINGKE Biotech were In Vitro or chemically synthesised as the TSINGKE Biotechnology web site is in Chinese only. I have deleted the Light microscopy experiment as I agree that the results look dubious. Accepted 2021-AUG-24 AT 11:03 BST AT 11:03 BST by LUANA Nature communications 2021 Only protein-protein interactions imex curation imex curation GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein. GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein. Nature communications 2021 imex curation Zhao M., Yu Y., Sun LM., Xing JQ., Li T., Zhu Y., Wang M., Yu Y., Xue W., Xia T., Cai H., Han QY., Yin X., Li WH., Li AL., Cui J., Yuan Z., Zhang R., Zhou T., Zhang XM., Li T. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus It is not known if the Viral RNA sequences obtained from TSINGKE Biotech were In Vitro or chemically synthesised as the TSINGKE Biotechnology web site is in Chinese only. I have deleted the Light microscopy experiment as I agree that the results look dubious. Supp. figure 4J:I have added the 20-50 vRNAs as separate interactors, as if I added them as a "Sufficient to bind" as a region on the 60nt vRNA, it would not be clear that the 60nt molecule was used for all the other figures. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Zhao M., Yu Y., Sun LM., Xing JQ., Li T., Zhu Y., Wang M., Yu Y., Xue W., Xia T., Cai H., Han QY., Yin X., Li WH., Li AL., Cui J., Yuan Z., Zhang R., Zhou T., Zhang XM., Li T. Virus - Publications including interactions involving viral proteins It is not known if the Viral RNA sequences obtained from TSINGKE Biotech were In Vitro or chemically synthesised as the TSINGKE Biotechnology web site is in Chinese only. I have deleted the Light microscopy experiment as I agree that the results look dubious. Accepted 2021-AUG-24 AT 11:03 BST AT 11:03 BST by LUANA Nature communications 2021 Only protein-protein interactions imex curation imex curation GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein. GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein. Nature communications 2021 imex curation Zhao M., Yu Y., Sun LM., Xing JQ., Li T., Zhu Y., Wang M., Yu Y., Xue W., Xia T., Cai H., Han QY., Yin X., Li WH., Li AL., Cui J., Yuan Z., Zhang R., Zhou T., Zhang XM., Li T. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus It is not known if the Viral RNA sequences obtained from TSINGKE Biotech were In Vitro or chemically synthesised as the TSINGKE Biotechnology web site is in Chinese only. I have deleted the Light microscopy experiment as I agree that the results look dubious. Supp. figure 4J:I have added the 20-50 vRNAs as separate interactors, as if I added them as a "Sufficient to bind" as a region on the 60nt vRNA, it would not be clear that the 60nt molecule was used for all the other figures. Only protein-protein interactions imex curation in vitro In vitro frap fluorescence recovery after photobleaching frap tag fluorescence tag visualisation by fluorescence tag fluorescence Zhao M., Yu Y., Sun LM., Xing JQ., Li T., Zhu Y., Wang M., Yu Y., Xue W., Xia T., Cai H., Han QY., Yin X., Li WH., Li AL., Cui J., Yuan Z., Zhang R., Zhou T., Zhang XM., Li T. Virus - Publications including interactions involving viral proteins It is not known if the Viral RNA sequences obtained from TSINGKE Biotech were In Vitro or chemically synthesised as the TSINGKE Biotechnology web site is in Chinese only. I have deleted the Light microscopy experiment as I agree that the results look dubious. Accepted 2021-AUG-24 AT 11:03 BST AT 11:03 BST by LUANA Nature communications 2021 Only protein-protein interactions imex curation imex curation GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein. GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein. Nature communications 2021 imex curation Zhao M., Yu Y., Sun LM., Xing JQ., Li T., Zhu Y., Wang M., Yu Y., Xue W., Xia T., Cai H., Han QY., Yin X., Li WH., Li AL., Cui J., Yuan Z., Zhang R., Zhou T., Zhang XM., Li T. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus It is not known if the Viral RNA sequences obtained from TSINGKE Biotech were In Vitro or chemically synthesised as the TSINGKE Biotechnology web site is in Chinese only. I have deleted the Light microscopy experiment as I agree that the results look dubious. Supp. figure 4J:I have added the 20-50 vRNAs as separate interactors, as if I added them as a "Sufficient to bind" as a region on the 60nt vRNA, it would not be clear that the 60nt molecule was used for all the other figures. Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Zhao M., Yu Y., Sun LM., Xing JQ., Li T., Zhu Y., Wang M., Yu Y., Xue W., Xia T., Cai H., Han QY., Yin X., Li WH., Li AL., Cui J., Yuan Z., Zhang R., Zhou T., Zhang XM., Li T. Virus - Publications including interactions involving viral proteins It is not known if the Viral RNA sequences obtained from TSINGKE Biotech were In Vitro or chemically synthesised as the TSINGKE Biotechnology web site is in Chinese only. I have deleted the Light microscopy experiment as I agree that the results look dubious. Accepted 2021-AUG-24 AT 11:03 BST AT 11:03 BST by LUANA Nature communications 2021 Only protein-protein interactions imex curation imex curation GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein. GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein. Nature communications 2021 imex curation Zhao M., Yu Y., Sun LM., Xing JQ., Li T., Zhu Y., Wang M., Yu Y., Xue W., Xia T., Cai H., Han QY., Yin X., Li WH., Li AL., Cui J., Yuan Z., Zhang R., Zhou T., Zhang XM., Li T. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus It is not known if the Viral RNA sequences obtained from TSINGKE Biotech were In Vitro or chemically synthesised as the TSINGKE Biotechnology web site is in Chinese only. I have deleted the Light microscopy experiment as I agree that the results look dubious. Supp. figure 4J:I have added the 20-50 vRNAs as separate interactors, as if I added them as a "Sufficient to bind" as a region on the 60nt vRNA, it would not be clear that the 60nt molecule was used for all the other figures. Only protein-protein interactions imex curation in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa tag visualisation tag visualisation Zhao M., Yu Y., Sun LM., Xing JQ., Li T., Zhu Y., Wang M., Yu Y., Xue W., Xia T., Cai H., Han QY., Yin X., Li WH., Li AL., Cui J., Yuan Z., Zhang R., Zhou T., Zhang XM., Li T. Virus - Publications including interactions involving viral proteins It is not known if the Viral RNA sequences obtained from TSINGKE Biotech were In Vitro or chemically synthesised as the TSINGKE Biotechnology web site is in Chinese only. I have deleted the Light microscopy experiment as I agree that the results look dubious. Accepted 2021-AUG-24 AT 11:03 BST AT 11:03 BST by LUANA Nature communications 2021 Only protein-protein interactions imex curation imex curation GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein. GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein. Nature communications 2021 imex curation Zhao M., Yu Y., Sun LM., Xing JQ., Li T., Zhu Y., Wang M., Yu Y., Xue W., Xia T., Cai H., Han QY., Yin X., Li WH., Li AL., Cui J., Yuan Z., Zhang R., Zhou T., Zhang XM., Li T. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus It is not known if the Viral RNA sequences obtained from TSINGKE Biotech were In Vitro or chemically synthesised as the TSINGKE Biotechnology web site is in Chinese only. I have deleted the Light microscopy experiment as I agree that the results look dubious. Supp. figure 4J:I have added the 20-50 vRNAs as separate interactors, as if I added them as a "Sufficient to bind" as a region on the 60nt vRNA, it would not be clear that the 60nt molecule was used for all the other figures. Only protein-protein interactions imex curation human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. frap fluorescence recovery after photobleaching frap tag fluorescence tag visualisation by fluorescence tag fluorescence Zhao M., Yu Y., Sun LM., Xing JQ., Li T., Zhu Y., Wang M., Yu Y., Xue W., Xia T., Cai H., Han QY., Yin X., Li WH., Li AL., Cui J., Yuan Z., Zhang R., Zhou T., Zhang XM., Li T. Virus - Publications including interactions involving viral proteins It is not known if the Viral RNA sequences obtained from TSINGKE Biotech were In Vitro or chemically synthesised as the TSINGKE Biotechnology web site is in Chinese only. I have deleted the Light microscopy experiment as I agree that the results look dubious. Accepted 2021-AUG-24 AT 11:03 BST AT 11:03 BST by LUANA Nature communications 2021 Only protein-protein interactions imex curation imex curation 20-nt vrna rna ribonucleic acid RNA rna SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV vrna_3'utr rna ribonucleic acid RNA rna SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV poly(i:c) poly(I:C) 5'-Inosinic acid, homopolymer, complex with 5'-cytidylic acid homopolymer (1:1) PI:C Poly C poly I Poly I-C poly I:C Poly I:poly C Poly IC Poly rI:poly rC Poly(cytidylic acid)-poly(inosinic acid) Poly(I).poly(C) Poly(I).poly(C) complex poly(I).poly(C) Poly(I+C) Poly(rI).(rC) Poly(rI).poly(rC) Polycytidylic-polyinosinic acid Polyinosinic acid:polycytidylic acid Polyinosinic-polycytidylic acid polyinosinic:polycytidylic acid Polyriboinosinic-polyribocytidylic acid small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) 55-nt_vrna rna ribonucleic acid RNA rna SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV gallocatechin gallate Gallocatechin gallate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) 60-nt vrna rna ribonucleic acid RNA rna SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV 30-nt vrna rna ribonucleic acid RNA rna SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV dsrna-3'utr ds rna ds rna double stranded ribonucleic acid SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV ncap_sars2 Nucleoprotein N Nucleocapsid protein protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA 50-nt vrna rna ribonucleic acid RNA rna SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV 5′ppp-dsrna ds rna ds rna double stranded ribonucleic acid synthetic construct synthetic construct GCAUGCGACCUCUGUUUGA 40-nt vrna rna ribonucleic acid RNA rna SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV 60_nt vrna-n-1 1 14 unspecified role unspecified role neutral component neutral component nsp1_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region cy5 label cy5 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 17 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0DTC9:p.Arg203_Gly204delinsLysArg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain RG KR Mon Jun 28 17:32:52 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg203_Gly204delinsLysArg' ecoli Escherichia coli (strain K12) direct interaction direct interaction 1 C/D,3D, 4C, Supp. figure 4J, 5E None of the truncated N variants can undergo LLPS (Fig. 3d–h) 60_nt vrna-n-3 1 17 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0DTC9:p.Arg203_Gly204delinsLysArg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain RG KR Mon Jun 28 17:36:26 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg203_Gly204delinsLysArg' ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role ancillary ancillary nsp1_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 17 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0DTC9:p.Arg203_Gly204delinsLysArg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain RG KR Mon Jun 28 16:57:44 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg203_Gly204delinsLysArg' ecoli Escherichia coli (strain K12) direct interaction direct interaction 1 E,3G, 4F, Supp. fig 3C, 4 A/D/G None of the truncated N variants can undergo LLPS (Fig. 3d–h) pH > ~7.0 [NaCl] ~>physiological (150mM/L) n-n-62 5 17 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 17 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 1 F The photo-bleached fluorescence signal of N-RNA droplets can be recovered within seconds. n-n-63 3 17 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 17 unspecified role unspecified role neutral component neutral component C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 2 B/D, 6A/D, Supp. figure 7 D/F poly(I:C) -CHEBI:84491 vRNA 3′ UTR (NC_045512.2 residues 29675-29903). Transfection of N-expressing cells with poly(I:C) or the vRNA (3′ UTR), resulted in the formation of N protein condensates. GCG (-)-gallocatechin gallate, a polyphenol from green tea, CHEBI:156271. 60-nt vrna (NC_045512.2, nsp1_region residues 335-394) . 5’ triphosphate_5’ppp-dsRNA (5'- pppGCAUGCGACCUCUGUUUGA-3') 55nt-vRNA (5' region)[residues 1-55, NC_045512.2] dsRNA -3'prime (NC_045512.2 residues 29675-29903) EGCG - Epigallocatechin gallate -the most abundant catechin in (green) tea CHEBI:4806. EGCG had much weaker effect on the inhibition of N-RNA condensation than GCG (-)-gallocatechin gallate. n-n-64 3 10 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region cy5 label cy5 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined vRNA_3'UTR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 17 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range direct interaction direct interaction 2C, 6 C Transfection of N-expressing cells with poly(I:C) or the vRNA (3′ UTR), resulted in the formation of N protein condensates. I have added the viral RNA as "over-expressed" as both Methods and text state it was "transfected" even if image text states "treated-with" -which implies an In Vitro experiment. (prep:it was in-vitro translated) Figure 6C: I can see a small amount of RED/cy dye on the image - hence added as an interaction. GCG (-)-gallocatechin gallate (GCG), a polyphenol from green tea, CHEBI:156271. n-n-65 2 17 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 17 unspecified role unspecified role neutral component neutral component C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 2 D poly(I:C) -CHEBI:84491 vRNA 3′ UTR (NC_045512.2 residues 29675-29903). Transfection of N-expressing cells with poly(I:C) or the vRNA (3′ UTR), resulted in the formation of N protein condensates. n-n-66 8 17 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 17 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 2 E poly(I:C) -CHEBI:84491 55_nt_vrna-n-1 7 12 unspecified role unspecified role bait bait 55nt-vrna (5' region) sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region cy3 label cy3 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 17 unspecified role unspecified role prey prey purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 3C, 6F, Supp. figure 3B Deletion of any domain disrupted the RNA-binding ability of N protein. GCG (-)-gallocatechin gallate (GCG), a polyphenol from green tea, CHEBI:156271. 55_nt_vrna-n-2 7 12 unspecified role unspecified role bait bait 55nt-vrna (5' region) sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region cy3 label cy3 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 17 unspecified role unspecified role prey prey purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 13 inhibitor inhibitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 6F GCG (-)-gallocatechin gallate (GCG), a polyphenol from green tea, CHEBI:156271. gallocatechin gallate-n-1 4 17 unspecified role unspecified role prey prey purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9inse 9inse 13 unspecified role unspecified role bait bait chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 6G gallocatechin gallate-n-2 6 17 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma 13 unspecified role unspecified role bait bait chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association 6H n-vrna_3'utr-2 1 10 unspecified role unspecified role ancillary ancillary purified purified vRNA_3'UTR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 17 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 17 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. figure 3C n-vrna_3'utr-1 1 12 unspecified role unspecified role ancillary ancillary purified purified 55nt-vrna (5' region) sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 17 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 17 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. figure 3C 5ppp_dsrna-n-1 1 19 unspecified role unspecified role ancillary ancillary purified purified 5’ guanosine-triphosphate rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain synthetic construct synthetic construct 17 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 17 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. figure 3C n-vrna_3'utr-3 1 16 unspecified role unspecified role ancillary ancillary purified purified vRNA_3'UTR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 17 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 17 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. figure 3C n-poly(i:c)-1 1 17 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role ancillary ancillary purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 17 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. figure 3C 20_nt vrna-n-1 1 9 unspecified role unspecified role neutral component neutral component nsp1_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region cy5 label cy5 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 17 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. figure 4J 30nt vrna-n-1 1 15 unspecified role unspecified role neutral component neutral component nsp1_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region cy5 label cy5 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 17 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. figure 4J 50_nt vrna-n-1 1 17 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 18 unspecified role unspecified role neutral component neutral component nsp1_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region cy5 label cy5 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Supp. figure 4J 40_nt vrna-n-2 1 17 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 20 unspecified role unspecified role neutral component neutral component nsp1_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region cy5 label cy5 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Supp. figure 4J