DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Cryo-EM structure of the full-length WzmWzt ABC transporter required for lipid-linked O antigen transport. Cryo-EM structure of the full-length WzmWzt ABC transporter required for lipid-linked O antigen transport. Proceedings of the National Academy of Sciences of the United States of America 2021 imex curation Caffalette CA., Zimmer J. jochen_zimmer@virginia.edu. 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) comigration in sds comigration in sds page comigration in sds experimental particp experimental participant identification experimental particp Caffalette CA., Zimmer J. jochen_zimmer@virginia.edu. Accepted 2021-MAR-04 AT 11:59 GMT AT 11:59 GMT by PPORRAS Proceedings of the National Academy of Sciences of the United States of America 2021 Only protein-protein interactions imex curation imex curation Cryo-EM structure of the full-length WzmWzt ABC transporter required for lipid-linked O antigen transport. Cryo-EM structure of the full-length WzmWzt ABC transporter required for lipid-linked O antigen transport. Proceedings of the National Academy of Sciences of the United States of America 2021 imex curation Caffalette CA., Zimmer J. jochen_zimmer@virginia.edu. 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Caffalette CA., Zimmer J. jochen_zimmer@virginia.edu. Accepted 2021-MAR-04 AT 11:59 GMT AT 11:59 GMT by PPORRAS Proceedings of the National Academy of Sciences of the United States of America 2021 Only protein-protein interactions imex curation imex curation Cryo-EM structure of the full-length WzmWzt ABC transporter required for lipid-linked O antigen transport. Cryo-EM structure of the full-length WzmWzt ABC transporter required for lipid-linked O antigen transport. Proceedings of the National Academy of Sciences of the United States of America 2021 imex curation Caffalette CA., Zimmer J. jochen_zimmer@virginia.edu. 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) 3d-em-single electron microscopy 3D single particle reconstruction 3D-EM-single predetermined predetermined participant predetermined Caffalette CA., Zimmer J. jochen_zimmer@virginia.edu. Accepted 2021-MAR-04 AT 11:59 GMT AT 11:59 GMT by PPORRAS Proceedings of the National Academy of Sciences of the United States of America 2021 Only protein-protein interactions imex curation IMEx imex curation o67182_aquae Transport permease protein abcT3 aq_1095 protein protein aquae Aquifex aeolicus (strain VF5) MNLSLILELVRQEIKNRYADTVLGIWWAFLWPILLVLIYTLIFSHLIGAKLGHENTVYAYSIYLSSGIFPWFFFSNSLSRITGIFTEKKFLFTKIPIRLEVFPVVVIISELINYLIGISLVTLISFITLGFEGIKYFYLFPVALYLMIVYSFSIGMVLGTLNVFFRDIKEIIGVFLQIFFWFTPIVYTLDILPPFVKKLIYYNPMYPVVSIHHLVFVNYLDLHLYSLLGFLLASPLVFFVSYYFFKKLEKDIKDFA C22DB4E6A7F6BDD9 o67181_aquae abcT4 aq_1094 protein protein aquae Aquifex aeolicus (strain VF5) MMIRVFDVWKKYKYYKKPQDRLKEIIFRKPFHEELWVLKGINLEIEKGEVLGIVGPNGAGKSTLLKVITGVTEPDKGFVERSGKVVGLLELGTGFNYELSGLENIYVNASLLGLSRREIDEKLESIIEFSELDDFINKPLKTYSSGMIMRLAFSIAIHTEPECFIIDEALAVGDAHFQQKCFRKLKEHKQKGGSIIFVSHDMNAVKILCDRAILLHKGEIIEEGSPETVTQAYYKLMASLENKEGITFLQNGYGNFKAVIKEVRLKSEHGYTNNFPSGDTLFIELDVEAKEDLQDVVAGILIRDRFGQDIFGINTYLMEKKVELKKGKYLFTFKMPLNLAPGKYTLTVALHKGMDHAQECYHWIDNVCNFEVNGFKKEQFVGVCYLPTEFNYRKIP 351D3A01A04B33F9 caffalette-2021-1 3 4 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified ecoli Escherichia coli (strain K12) 5 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified O67181:p.Glu168Gln E167Q mutation mutation mutation certain certain sequence position certain certain certain sequence position certain E Q Mon Mar 01 11:33:09 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'o67181_aquae:p.Glu168Gln' His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 1, Fig 2, Table S1 abct3-abct4-1 2 4 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified ecoli Escherichia coli (strain K12) 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified O67181:p.Glu168Gln E167Q mutation mutation mutation certain certain sequence position certain certain certain sequence position certain E Q Mon Mar 01 10:43:38 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'o67181_aquae:p.Glu168Gln' His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig S1a abct4-2 1 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified O67181:p.Glu168Gln E167Q mutation mutation mutation certain certain sequence position certain certain certain sequence position certain E Q Mon Mar 01 11:03:24 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'o67181_aquae:p.Glu168Gln' His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig S1a