IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity. Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity. Nature 2020 imex curation Shin D., Mukherjee R., Grewe D., Bojkova D., Baek K., Bhattacharya A., Schulz L., Widera M., Mehdipour AR., Tascher G., Geurink PP., Wilhelm A., van der Heden van Noort GJ., Ovaa H., Müller S., Knobeloch KP., Rajalingam K., Schulman BA., Cinatl J., Hummer G., Ciesek S., Dikic I. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus 8/01/21 -Figure 4A and elsewhere:As they are quoting a C111S mutation I assume they are still using the UBL domain of the viral proteins. The MERS PDBe entry (5W8U) refers to an "2'-O-methyltransferase" - wrong viral protein, but I have Blasted stated PDBe sequence to get the sequence in relation to MERS NSp3, reviewed entry - K9N7C7. It does map OK to Nsp3 - not Nsp16 - the methyltransferase. I cannot find any results for Bio-layer Interferometry- if you find any experimental data please send back, and maybe I can add kinetic data from Table ?? As no source or species origin is stated for (K48) Ubiquitin, Human UBC ubiquitin has been used as an example of this molecule. SARS and MERS PLpro UBL domains have been derived from PDBe entries 3mj5 and 5W8U, as quoted under "Plasmids construction". The authors have stated that IRF3-myc is of Human origin. dikic@biochem2.uni-frankfurt.de 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-caco2 Human Caucasian colon adenocarcinoma caco2 Human Caucasian colon adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Shin D., Mukherjee R., Grewe D., Bojkova D., Baek K., Bhattacharya A., Schulz L., Widera M., Mehdipour AR., Tascher G., Geurink PP., Wilhelm A., van der Heden van Noort GJ., Ovaa H., Müller S., Knobeloch KP., Rajalingam K., Schulman BA., Cinatl J., Hummer G., Ciesek S., Dikic I. Virus - Publications including interactions involving viral proteins 8/01/21 -Figure 4A and elsewhere:As they are quoting a C111S mutation I assume they are still using the UBL domain of the viral proteins. The MERS PDBe entry (5W8U) refers to an "2'-O-methyltransferase" - wrong viral protein, but I have Blasted stated PDBe sequence to get the sequence in relation to MERS NSp3, reviewed entry - K9N7C7. It does map OK to Nsp3 - not Nsp16 - the methyltransferase. I cannot find any results for Bio-layer Interferometry- if you find any experimental data please send back, and maybe I can add kinetic data from Table ?? As no source or species origin is stated for (K48) Ubiquitin, Human UBC ubiquitin has been used as an example of this molecule. SARS and MERS PLpro UBL domains have been derived from PDBe entries 3mj5 and 5W8U, as quoted under "Plasmids construction". The authors have stated that IRF3-myc is of Human origin. dikic@biochem2.uni-frankfurt.de Accepted 2021-FEB-23 AT 09:09 GMT AT 09:09 GMT by KALPANAP Nature 2020 Only protein-protein interactions imex curation imex curation Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity. Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity. Nature 2020 imex curation Shin D., Mukherjee R., Grewe D., Bojkova D., Baek K., Bhattacharya A., Schulz L., Widera M., Mehdipour AR., Tascher G., Geurink PP., Wilhelm A., van der Heden van Noort GJ., Ovaa H., Müller S., Knobeloch KP., Rajalingam K., Schulman BA., Cinatl J., Hummer G., Ciesek S., Dikic I. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus 8/01/21 -Figure 4A and elsewhere:As they are quoting a C111S mutation I assume they are still using the UBL domain of the viral proteins. The MERS PDBe entry (5W8U) refers to an "2'-O-methyltransferase" - wrong viral protein, but I have Blasted stated PDBe sequence to get the sequence in relation to MERS NSp3, reviewed entry - K9N7C7. It does map OK to Nsp3 - not Nsp16 - the methyltransferase. I cannot find any results for Bio-layer Interferometry- if you find any experimental data please send back, and maybe I can add kinetic data from Table ?? As no source or species origin is stated for (K48) Ubiquitin, Human UBC ubiquitin has been used as an example of this molecule. SARS and MERS PLpro UBL domains have been derived from PDBe entries 3mj5 and 5W8U, as quoted under "Plasmids construction". The authors have stated that IRF3-myc is of Human origin. dikic@biochem2.uni-frankfurt.de 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro probe interaction assay probe interaction assay probe interaction assay weight silver stain molecular weight estimation by silver staining weight silver stain Shin D., Mukherjee R., Grewe D., Bojkova D., Baek K., Bhattacharya A., Schulz L., Widera M., Mehdipour AR., Tascher G., Geurink PP., Wilhelm A., van der Heden van Noort GJ., Ovaa H., Müller S., Knobeloch KP., Rajalingam K., Schulman BA., Cinatl J., Hummer G., Ciesek S., Dikic I. Virus - Publications including interactions involving viral proteins 8/01/21 -Figure 4A and elsewhere:As they are quoting a C111S mutation I assume they are still using the UBL domain of the viral proteins. The MERS PDBe entry (5W8U) refers to an "2'-O-methyltransferase" - wrong viral protein, but I have Blasted stated PDBe sequence to get the sequence in relation to MERS NSp3, reviewed entry - K9N7C7. It does map OK to Nsp3 - not Nsp16 - the methyltransferase. I cannot find any results for Bio-layer Interferometry- if you find any experimental data please send back, and maybe I can add kinetic data from Table ?? The propargylamide warhead of the K48-Ub2 or SG15–Prg probe covalently binds to the Nsp3 active site cysteine. As no source or species origin is stated for (K48) Ubiquitin, Human UBC ubiquitin has been used as an example of this molecule. SARS and MERS PLpro UBL domains have been derived from PDBe entries 3mj5 and 5W8U, as quoted under "Plasmids construction". The authors have stated that IRF3-myc is of Human origin. dikic@biochem2.uni-frankfurt.de Accepted 2021-FEB-23 AT 09:09 GMT AT 09:09 GMT by KALPANAP Nature 2020 Only protein-protein interactions imex curation imex curation Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity. Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity. Nature 2020 imex curation Shin D., Mukherjee R., Grewe D., Bojkova D., Baek K., Bhattacharya A., Schulz L., Widera M., Mehdipour AR., Tascher G., Geurink PP., Wilhelm A., van der Heden van Noort GJ., Ovaa H., Müller S., Knobeloch KP., Rajalingam K., Schulman BA., Cinatl J., Hummer G., Ciesek S., Dikic I. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus 8/01/21 -Figure 4A and elsewhere:As they are quoting a C111S mutation I assume they are still using the UBL domain of the viral proteins. The MERS PDBe entry (5W8U) refers to an "2'-O-methyltransferase" - wrong viral protein, but I have Blasted stated PDBe sequence to get the sequence in relation to MERS NSp3, reviewed entry - K9N7C7. It does map OK to Nsp3 - not Nsp16 - the methyltransferase. I cannot find any results for Bio-layer Interferometry- if you find any experimental data please send back, and maybe I can add kinetic data from Table ?? As no source or species origin is stated for (K48) Ubiquitin, Human UBC ubiquitin has been used as an example of this molecule. SARS and MERS PLpro UBL domains have been derived from PDBe entries 3mj5 and 5W8U, as quoted under "Plasmids construction". The authors have stated that IRF3-myc is of Human origin. dikic@biochem2.uni-frankfurt.de 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Shin D., Mukherjee R., Grewe D., Bojkova D., Baek K., Bhattacharya A., Schulz L., Widera M., Mehdipour AR., Tascher G., Geurink PP., Wilhelm A., van der Heden van Noort GJ., Ovaa H., Müller S., Knobeloch KP., Rajalingam K., Schulman BA., Cinatl J., Hummer G., Ciesek S., Dikic I. Virus - Publications including interactions involving viral proteins 8/01/21 -Figure 4A and elsewhere:As they are quoting a C111S mutation I assume they are still using the UBL domain of the viral proteins. The MERS PDBe entry (5W8U) refers to an "2'-O-methyltransferase" - wrong viral protein, but I have Blasted stated PDBe sequence to get the sequence in relation to MERS NSp3, reviewed entry - K9N7C7. It does map OK to Nsp3 - not Nsp16 - the methyltransferase. I cannot find any results for Bio-layer Interferometry- if you find any experimental data please send back, and maybe I can add kinetic data from Table ?? Cells were stimulated with IFN-alpha to mimic an infection scenario. According to Ref. 64 (pmid: 31812349) TMT and SILAC labelling were used. As no source or species origin is stated for (K48) Ubiquitin, Human UBC ubiquitin has been used as an example of this molecule. SARS and MERS PLpro UBL domains have been derived from PDBe entries 3mj5 and 5W8U, as quoted under "Plasmids construction". The authors have stated that IRF3-myc is of Human origin. dikic@biochem2.uni-frankfurt.de Accepted 2021-FEB-23 AT 09:09 GMT AT 09:09 GMT by KALPANAP Nature 2020 Only protein-protein interactions imex curation imex curation Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity. Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity. Nature 2020 imex curation Shin D., Mukherjee R., Grewe D., Bojkova D., Baek K., Bhattacharya A., Schulz L., Widera M., Mehdipour AR., Tascher G., Geurink PP., Wilhelm A., van der Heden van Noort GJ., Ovaa H., Müller S., Knobeloch KP., Rajalingam K., Schulman BA., Cinatl J., Hummer G., Ciesek S., Dikic I. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus 8/01/21 -Figure 4A and elsewhere:As they are quoting a C111S mutation I assume they are still using the UBL domain of the viral proteins. The MERS PDBe entry (5W8U) refers to an "2'-O-methyltransferase" - wrong viral protein, but I have Blasted stated PDBe sequence to get the sequence in relation to MERS NSp3, reviewed entry - K9N7C7. It does map OK to Nsp3 - not Nsp16 - the methyltransferase. I cannot find any results for Bio-layer Interferometry- if you find any experimental data please send back, and maybe I can add kinetic data from Table ?? As no source or species origin is stated for (K48) Ubiquitin, Human UBC ubiquitin has been used as an example of this molecule. SARS and MERS PLpro UBL domains have been derived from PDBe entries 3mj5 and 5W8U, as quoted under "Plasmids construction". The authors have stated that IRF3-myc is of Human origin. dikic@biochem2.uni-frankfurt.de 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Shin D., Mukherjee R., Grewe D., Bojkova D., Baek K., Bhattacharya A., Schulz L., Widera M., Mehdipour AR., Tascher G., Geurink PP., Wilhelm A., van der Heden van Noort GJ., Ovaa H., Müller S., Knobeloch KP., Rajalingam K., Schulman BA., Cinatl J., Hummer G., Ciesek S., Dikic I. Virus - Publications including interactions involving viral proteins 8/01/21 -Figure 4A and elsewhere:As they are quoting a C111S mutation I assume they are still using the UBL domain of the viral proteins. The MERS PDBe entry (5W8U) refers to an "2'-O-methyltransferase" - wrong viral protein, but I have Blasted stated PDBe sequence to get the sequence in relation to MERS NSp3, reviewed entry - K9N7C7. It does map OK to Nsp3 - not Nsp16 - the methyltransferase. I cannot find any results for Bio-layer Interferometry- if you find any experimental data please send back, and maybe I can add kinetic data from Table ?? As no source or species origin is stated for (K48) Ubiquitin, Human UBC ubiquitin has been used as an example of this molecule. SARS and MERS PLpro UBL domains have been derived from PDBe entries 3mj5 and 5W8U, as quoted under "Plasmids construction". The authors have stated that IRF3-myc is of Human origin. dikic@biochem2.uni-frankfurt.de Accepted 2021-FEB-23 AT 09:09 GMT AT 09:09 GMT by KALPANAP Nature 2020 Only protein-protein interactions imex curation imex curation Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity. Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity. Nature 2020 imex curation Shin D., Mukherjee R., Grewe D., Bojkova D., Baek K., Bhattacharya A., Schulz L., Widera M., Mehdipour AR., Tascher G., Geurink PP., Wilhelm A., van der Heden van Noort GJ., Ovaa H., Müller S., Knobeloch KP., Rajalingam K., Schulman BA., Cinatl J., Hummer G., Ciesek S., Dikic I. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus 8/01/21 -Figure 4A and elsewhere:As they are quoting a C111S mutation I assume they are still using the UBL domain of the viral proteins. The MERS PDBe entry (5W8U) refers to an "2'-O-methyltransferase" - wrong viral protein, but I have Blasted stated PDBe sequence to get the sequence in relation to MERS NSp3, reviewed entry - K9N7C7. It does map OK to Nsp3 - not Nsp16 - the methyltransferase. I cannot find any results for Bio-layer Interferometry- if you find any experimental data please send back, and maybe I can add kinetic data from Table ?? As no source or species origin is stated for (K48) Ubiquitin, Human UBC ubiquitin has been used as an example of this molecule. SARS and MERS PLpro UBL domains have been derived from PDBe entries 3mj5 and 5W8U, as quoted under "Plasmids construction". The authors have stated that IRF3-myc is of Human origin. dikic@biochem2.uni-frankfurt.de 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro cleavage cleavage assay cleavage tag fluorescence tag visualisation by fluorescence tag fluorescence Shin D., Mukherjee R., Grewe D., Bojkova D., Baek K., Bhattacharya A., Schulz L., Widera M., Mehdipour AR., Tascher G., Geurink PP., Wilhelm A., van der Heden van Noort GJ., Ovaa H., Müller S., Knobeloch KP., Rajalingam K., Schulman BA., Cinatl J., Hummer G., Ciesek S., Dikic I. Virus - Publications including interactions involving viral proteins 8/01/21 -Figure 4A and elsewhere:As they are quoting a C111S mutation I assume they are still using the UBL domain of the viral proteins. The MERS PDBe entry (5W8U) refers to an "2'-O-methyltransferase" - wrong viral protein, but I have Blasted stated PDBe sequence to get the sequence in relation to MERS NSp3, reviewed entry - K9N7C7. It does map OK to Nsp3 - not Nsp16 - the methyltransferase. I cannot find any results for Bio-layer Interferometry- if you find any experimental data please send back, and maybe I can add kinetic data from Table ?? An 7-amido-4-methylcoumarine (AMC) probe emits fluorescence upon cleavage, enabling the monitoring of the kinetics of the protease activity. As no source or species origin is stated for (K48) Ubiquitin, Human UBC ubiquitin has been used as an example of this molecule. SARS and MERS PLpro UBL domains have been derived from PDBe entries 3mj5 and 5W8U, as quoted under "Plasmids construction". The authors have stated that IRF3-myc is of Human origin. dikic@biochem2.uni-frankfurt.de Accepted 2021-FEB-23 AT 09:09 GMT AT 09:09 GMT by KALPANAP Nature 2020 Only protein-protein interactions imex curation imex curation Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity. Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity. Nature 2020 imex curation Shin D., Mukherjee R., Grewe D., Bojkova D., Baek K., Bhattacharya A., Schulz L., Widera M., Mehdipour AR., Tascher G., Geurink PP., Wilhelm A., van der Heden van Noort GJ., Ovaa H., Müller S., Knobeloch KP., Rajalingam K., Schulman BA., Cinatl J., Hummer G., Ciesek S., Dikic I. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus 8/01/21 -Figure 4A and elsewhere:As they are quoting a C111S mutation I assume they are still using the UBL domain of the viral proteins. The MERS PDBe entry (5W8U) refers to an "2'-O-methyltransferase" - wrong viral protein, but I have Blasted stated PDBe sequence to get the sequence in relation to MERS NSp3, reviewed entry - K9N7C7. It does map OK to Nsp3 - not Nsp16 - the methyltransferase. I cannot find any results for Bio-layer Interferometry- if you find any experimental data please send back, and maybe I can add kinetic data from Table ?? As no source or species origin is stated for (K48) Ubiquitin, Human UBC ubiquitin has been used as an example of this molecule. SARS and MERS PLpro UBL domains have been derived from PDBe entries 3mj5 and 5W8U, as quoted under "Plasmids construction". The authors have stated that IRF3-myc is of Human origin. dikic@biochem2.uni-frankfurt.de 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Shin D., Mukherjee R., Grewe D., Bojkova D., Baek K., Bhattacharya A., Schulz L., Widera M., Mehdipour AR., Tascher G., Geurink PP., Wilhelm A., van der Heden van Noort GJ., Ovaa H., Müller S., Knobeloch KP., Rajalingam K., Schulman BA., Cinatl J., Hummer G., Ciesek S., Dikic I. Virus - Publications including interactions involving viral proteins 8/01/21 -Figure 4A and elsewhere:As they are quoting a C111S mutation I assume they are still using the UBL domain of the viral proteins. The MERS PDBe entry (5W8U) refers to an "2'-O-methyltransferase" - wrong viral protein, but I have Blasted stated PDBe sequence to get the sequence in relation to MERS NSp3, reviewed entry - K9N7C7. It does map OK to Nsp3 - not Nsp16 - the methyltransferase. I cannot find any results for Bio-layer Interferometry- if you find any experimental data please send back, and maybe I can add kinetic data from Table ?? Cells were stimulated with IFN-alpha to mimic an infection scenario. As no source or species origin is stated for (K48) Ubiquitin, Human UBC ubiquitin has been used as an example of this molecule. SARS and MERS PLpro UBL domains have been derived from PDBe entries 3mj5 and 5W8U, as quoted under "Plasmids construction". The authors have stated that IRF3-myc is of Human origin. dikic@biochem2.uni-frankfurt.de Accepted 2021-FEB-23 AT 09:09 GMT AT 09:09 GMT by KALPANAP Nature 2020 Only protein-protein interactions imex curation imex curation Mock publication and experiment for abstract interactions that are not interaction evidences. Mock publication and experiment for abstract interactions that are not interaction evidences. unspecified method Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity. Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity. Nature 2020 imex curation Shin D., Mukherjee R., Grewe D., Bojkova D., Baek K., Bhattacharya A., Schulz L., Widera M., Mehdipour AR., Tascher G., Geurink PP., Wilhelm A., van der Heden van Noort GJ., Ovaa H., Müller S., Knobeloch KP., Rajalingam K., Schulman BA., Cinatl J., Hummer G., Ciesek S., Dikic I. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus 8/01/21 -Figure 4A and elsewhere:As they are quoting a C111S mutation I assume they are still using the UBL domain of the viral proteins. The MERS PDBe entry (5W8U) refers to an "2'-O-methyltransferase" - wrong viral protein, but I have Blasted stated PDBe sequence to get the sequence in relation to MERS NSp3, reviewed entry - K9N7C7. It does map OK to Nsp3 - not Nsp16 - the methyltransferase. I cannot find any results for Bio-layer Interferometry- if you find any experimental data please send back, and maybe I can add kinetic data from Table ?? As no source or species origin is stated for (K48) Ubiquitin, Human UBC ubiquitin has been used as an example of this molecule. SARS and MERS PLpro UBL domains have been derived from PDBe entries 3mj5 and 5W8U, as quoted under "Plasmids construction". The authors have stated that IRF3-myc is of Human origin. dikic@biochem2.uni-frankfurt.de 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro cleavage cleavage assay cleavage weight by comassie molecular weight estimation by coomasie staining weight by comassie Shin D., Mukherjee R., Grewe D., Bojkova D., Baek K., Bhattacharya A., Schulz L., Widera M., Mehdipour AR., Tascher G., Geurink PP., Wilhelm A., van der Heden van Noort GJ., Ovaa H., Müller S., Knobeloch KP., Rajalingam K., Schulman BA., Cinatl J., Hummer G., Ciesek S., Dikic I. Virus - Publications including interactions involving viral proteins Expression system for CULL8 taken from PDBe entry 6ttu (pmid:32051583). Interactions involving "CSN" have been captured using a complex containing only Csn7b. (Ref:48 -pmid: 22959436). 8/01/21 -Figure 4A and elsewhere:As they are quoting a C111S mutation I assume they are still using the UBL domain of the viral proteins. The MERS PDBe entry (5W8U) refers to an "2'-O-methyltransferase" - wrong viral protein, but I have Blasted stated PDBe sequence to get the sequence in relation to MERS NSp3, reviewed entry - K9N7C7. It does map OK to Nsp3 - not Nsp16 - the methyltransferase. I cannot find any results for Bio-layer Interferometry- if you find any experimental data please send back, and maybe I can add kinetic data from Table ?? As no source or species origin is stated for (K48) Ubiquitin, Human UBC ubiquitin has been used as an example of this molecule. SARS and MERS PLpro UBL domains have been derived from PDBe entries 3mj5 and 5W8U, as quoted under "Plasmids construction". The authors have stated that IRF3-myc is of Human origin. dikic@biochem2.uni-frankfurt.de Accepted 2021-FEB-23 AT 09:09 GMT AT 09:09 GMT by KALPANAP Nature 2020 Only protein-protein interactions imex curation imex curation Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity. Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity. Nature 2020 imex curation Shin D., Mukherjee R., Grewe D., Bojkova D., Baek K., Bhattacharya A., Schulz L., Widera M., Mehdipour AR., Tascher G., Geurink PP., Wilhelm A., van der Heden van Noort GJ., Ovaa H., Müller S., Knobeloch KP., Rajalingam K., Schulman BA., Cinatl J., Hummer G., Ciesek S., Dikic I. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus 8/01/21 -Figure 4A and elsewhere:As they are quoting a C111S mutation I assume they are still using the UBL domain of the viral proteins. The MERS PDBe entry (5W8U) refers to an "2'-O-methyltransferase" - wrong viral protein, but I have Blasted stated PDBe sequence to get the sequence in relation to MERS NSp3, reviewed entry - K9N7C7. It does map OK to Nsp3 - not Nsp16 - the methyltransferase. I cannot find any results for Bio-layer Interferometry- if you find any experimental data please send back, and maybe I can add kinetic data from Table ?? As no source or species origin is stated for (K48) Ubiquitin, Human UBC ubiquitin has been used as an example of this molecule. SARS and MERS PLpro UBL domains have been derived from PDBe entries 3mj5 and 5W8U, as quoted under "Plasmids construction". The authors have stated that IRF3-myc is of Human origin. dikic@biochem2.uni-frankfurt.de 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Shin D., Mukherjee R., Grewe D., Bojkova D., Baek K., Bhattacharya A., Schulz L., Widera M., Mehdipour AR., Tascher G., Geurink PP., Wilhelm A., van der Heden van Noort GJ., Ovaa H., Müller S., Knobeloch KP., Rajalingam K., Schulman BA., Cinatl J., Hummer G., Ciesek S., Dikic I. Virus - Publications including interactions involving viral proteins 8/01/21 -Figure 4A and elsewhere:As they are quoting a C111S mutation I assume they are still using the UBL domain of the viral proteins. The MERS PDBe entry (5W8U) refers to an "2'-O-methyltransferase" - wrong viral protein, but I have Blasted stated PDBe sequence to get the sequence in relation to MERS NSp3, reviewed entry - K9N7C7. It does map OK to Nsp3 - not Nsp16 - the methyltransferase. I cannot find any results for Bio-layer Interferometry- if you find any experimental data please send back, and maybe I can add kinetic data from Table ?? Cells were stimulated with IFN-alpha to mimic an infection scenario. As no source or species origin is stated for (K48) Ubiquitin, Human UBC ubiquitin has been used as an example of this molecule. SARS and MERS PLpro UBL domains have been derived from PDBe entries 3mj5 and 5W8U, as quoted under "Plasmids construction". The authors have stated that IRF3-myc is of Human origin. dikic@biochem2.uni-frankfurt.de Accepted 2021-FEB-23 AT 09:09 GMT AT 09:09 GMT by KALPANAP Nature 2020 Only protein-protein interactions imex curation imex curation k9n7c7-pro_0000422441 Papain-like proteinase rep ORF1ab polyprotein 1a-1b protein protein cvemc Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (Betacoronavirus England 1) Betacoronavirus England 1 Human coronavirus EMC APVKKVAFGGDQVHEVAAVRSVTVEYNIHAVLDTLLASSSLRTFVVDKSLSIEEFADVVKEQVSDLLVKLLRGMPIPDFDLDDFIDAPCYCFNAEGDASWSSTMIFSLHPVECDEECSEVEASDLEEGESECISETSTEQVDVSHEISDDEWAAAVDEAFPLDEAEDVTESVQEEAQPVEVPVEDIAQVVIADTLQETPVVSDTVEVPPQVVKLPSEPQTIQPEVKEVAPVYEADTEQTQSVTVKPKRLRKKRNVDPLSNFEHKVITECVTIVLGDAIQVAKCYGESVLVNAANTHLKHGGGIAGAINAASKGAVQKESDEYILAKGPLQVGDSVLLQGHSLAKNILHVVGPDARAKQDVSLLSKCYKAMNAYPLVVTPLVSAGIFGVKPAVSFDYLIREAKTRVLVVVNSQDVYKSLTIVDIPQSLTFSYDGLRGAIRKAKDYGFTVFVCTDNSANTKVLRNKGVDYTKKFLTVDGVQYYCYTSKDTLDDILQQANKSVGIISMPLGYVSHGLDLIQAGSVVRRVNVPYVCLLANKEQEAILMSEDVKLNPSEDFIKHVRTNGGYNSWHLVEGELLVQDLRLNKLLHWSDQTICYKDSVFYVVKNSTAFPFETLSACRAYLDSRTTQQLTIEVLVTVDGVNFRTVVLNNKNTYRSQLGCVFFNGADISDTIPDEKQNGHSLYLADNLTADETKALKELYGPVDPTFLHRFYSLKAAVHKWKMVVCDKVRSLKLSDNNCYLNAVIMTLDLLKDIKFVIPALQHAFMKHKGGDSTDFIALIMAYGNCTFGAPDDASRLLHTVLAKAELCCSARMVWREWCNVCGIKDVVLQGLKACCYVGVQTVEDLRARMTYVCQCGGERHRQIVEHTTPWLLLSGTPNEKLVTTSTAPDFVAFNVFQGIETAVGHYVHARLKGGLILKFDSGTVSKTSDWKCKVTDVLFPGQKYSSDCNVVRYSLDGNFRTEVDPDLSAFYVKDGKYFTSEPPVTYSPATILAGSVYTNSCLVSSDGQPGGDAISLSFNNLLGFDSSKPVTKKYTYSFLPKEDGDVLLAEFDTYDPIYKNGAMYKGKPILWVNKASYDTNLNKFNRASLRQIFDVAPIELENKFTPLSVESTPVEPPTVDVVALQQEMTIVKCKGLNKPFVKDNVSFVADDSGTPVVEYLSKEDLHTLYVDPKYQVIVLKDNVLSSMLRLHTVESGDINVVAASGSLTRKVKLLFRASFYFKEFATRTFTATTAVGSCIKSVVRHLGVTKGILTGCFSFVKMLFMLPLAYFSDSKLGTTEVKVSALKTAGVVTGNVVKQCCTAAVDLSMDKLRRVDWKSTLRLLLMLCTTMVLLSSVYHLYVFNQVLSSDVMFEDAQGLKKFYKEVRAYLGISSACDGLASAYRANSFDVPTFCANRSAMCNWCLISQDSITHYPALKMVQTHLSHYVLNIDWLWFAFETGLAYMLYTSAFNWLLLAGTLHYFFAQTSIFVDWRSYNYAVSSAFWLFTHIPMAGLVRMYNLLACLWLLRKFYQHVINGCKDTACLLCYKRNRLTRVEASTVVCGGKRTFYITANGGISFCRRHNWNCVDCDTAGVGNTFICEEVANDLTTALRRPINATDRSHYYVDSVTVKETVVQFNYRRDGQPFYERFPLCAFTNLDKLKFKEVCKTTTGIPEYNFIIYDSSDRGQESLARSACVYYSQVLCKSILLVDSSLVTSVGDSSEIATKMFDSFVNSFVSLYNVTRDKLEKLISTARDGVRRGDNFHSVLTTFIDAARGPAGVESDVETNEIVDSVQYAHKHDIQITNESYNNYVPSYVKPDSVSTSDLGSLIDCNAASVNQIVLRNSNGACIWNAAAYMKLSDALKRQIRIACRKCNLAFRLTTSKLRANDNILSVRFTANKIVGG 854 2740 532E0D4BDE146A6E spb4_human Serpin B4 Squamous cell carcinoma antigen 2 Leupin SERPINB4 SCCA2 PI11 Peptidase inhibitor 11 protein protein human Homo sapiens Human MNSLSEANTKFMFDLFQQFRKSKENNIFYSPISITSALGMVLLGAKDNTAQQISKVLHFDQVTENTTEKAATYHVDRSGNVHHQFQKLLTEFNKSTDAYELKIANKLFGEKTYQFLQEYLDAIKKFYQTSVESTDFANAPEESRKKINSWVESQTNEKIKNLFPDGTIGNDTTLVLVNAIYFKGQWENKFKKENTKEEKFWPNKNTYKSVQMMRQYNSFNFALLEDVQAKVLEIPYKGKDLSMIVLLPNEIDGLQKLEEKLTAEKLMEWTSLQNMRETCVDLHLPRFKMEESYDLKDTLRTMGMVNIFNGDADLSGMTWSHGLSVSKVLHKAFVEVTEEGVEAAAATAVVVVELSSPSTNEEFCCNHPFLFFIRQNKTNSILFYGRFSSP 04E213CD892587D5 ubp18_mouse Ubl carboxyl-terminal hydrolase 18 Usp18 Ubp43 43 kDa ISG15-specific protease ISG15-specific-processing protease Ubl thioesterase 18 protein protein mouse Mus musculus Mouse MGKGFGLLRKPCQSVVAEPQQYSALEEERTMKRKRVLSRDLCSAWDSPHGLVGLHNIGQTCCLNSLLQVFMMNMDFRMILKRITVPRSAEERKRSVPFQLLLLLEKMQDSRQKAVLPTELVQCLQKYNVPLFVQHDAAQLYLTIWNLTKDQITDTDLTERLQGLFTIWTQESLICVGCTAESSRRSKLLTLSLPLFDKDAKPLKTLEDALRCFVQPKELASSDMCCESCGEKTPWKQVLKLTHLPQTLTIHLMRFSARNSRTEKICHSVNFPQSLDFSQVLPTEEDLGDTKEQSEIHYELFAVIAHVGMADFGHYCAYIRNPVDGKWFCFNDSHVCWVTWKDVQCTYGNHRYRWRETAYLLVYTKTGS 19A4458FEB44CC22 cul1_human Cullin-1 CUL1 protein protein human Homo sapiens Human MSSTRSQNPHGLKQIGLDQIWDDLRAGIQQVYTRQSMAKSRYMELYTHVYNYCTSVHQSNQARGAGVPPSKSKKGQTPGGAQFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYTQQWEDYRFSSKVLNGICAYLNRHWVRRECDEGRKGIYEIYSLALVTWRDCLFRPLNKQVTNAVLKLIEKERNGETINTRLISGVVQSYVELGLNEDDAFAKGPTLTVYKESFESQFLADTERFYTRESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLHESTQDELARKCEQVLIEKHLEIFHTEFQNLLDADKNEDLGRMYNLVSRIQDGLGELKKLLETHIHNQGLAAIEKCGEAALNDPKMYVQTVLDVHKKYNALVMSAFNNDAGFVAALDKACGRFINNNAVTKMAQSSSKSPELLARYCDSLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQSCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYTVQQLTDSTQIKMDILAQVLQILLKSKLLVLEDENANVDEVELKPDTLIKLYLGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLGEVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA DIP protein Q13616 original sequence version: 144 DIP protein Q13616 original sequence version: 133 DIP protein Q13616 original sequence version: null DIP protein Q13616 original sequence version: 135 DIP protein Q13616 original sequence version: 98 DIP protein Q13616 original sequence version: 110 DIP protein Q13616 original sequence version: 146 DIP protein Q13616 original sequence version: 148 DIP protein Q13616 original sequence version: 100 DIP protein Q13616 original sequence version: 85 DIP protein Q13616 original sequence version: 114 DIP protein Q13616 original sequence version: 119 DIP protein Q13616 original sequence version: 136 DIP protein Q13616 original sequence version: 117 DIP protein Q13616 original sequence version: 99 DIP protein Q13616 original sequence version: 108 6625A1FFA7799BBA s10a9_human Protein S100-A9 S100 calcium-binding protein A9 Calgranulin-B Migration inhibitory factor-related protein 14 Leukocyte L1 complex heavy chain Calprotectin L1H subunit S100A9 CAGB CFAG MRP14 protein protein human Homo sapiens Human MTCKMSQLERNIETIINTFHQYSVKLGHPDTLNQGEFKELVRKDLQNFLKKENKNEKVIEHIMEDLDTNADKQLSFEEFIMLMARLTWASHEKMHEGDEGPGHHHKPGLGEGTP C3BE19729E14C078 nedd8_human NEDD8 Ubiquitin-like protein Nedd8 Neddylin Neural precursor cell expressed developmentally down-regulated protein 8 NEDD8 protein protein human Homo sapiens Human MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGGGGLRQ DIP protein Q15843 original sequence version: 103 DIP protein Q15843 original sequence version: 149 DIP protein Q15843 original sequence version: 58 DIP protein Q15843 original sequence version: 124 DIP protein Q15843 original sequence version: 95 DIP protein Q15843 original sequence version: 111 DC2FE102BE4725D2 csn7b_human COP9 signalosome complex subunit 7b COPS7B CSN7B JAB1-containing signalosome subunit 7b protein protein human Homo sapiens Human MAGEQKPSSNLLEQFILLAKGTSGSALTALISQVLEAPGVYVFGELLELANVQELAEGANAAYLQLLNLFAYGTYPDYIANKESLPELSTAQQNKLKHLTIVSLASRMKCIPYSVLLKDLEMRNLRELEDLIIEAVYTDIIQGKLDQRNQLLEVDFCIGRDIRKKDINNIVKTLHEWCDGCEAVLLGIEQQVLRANQYKENHNRTQQQVEAEVTNIKKTLKATASSSAQEMEQQLAERECPPHAEQRQPTKKMSKVKGLVSSRH DIP protein Q9H9Q2 original sequence version: 54 FF9279CEA6CB7707 spb3_human Serpin B3 SERPINB3 SCCA SCCA1 Squamous cell carcinoma antigen 1 Protein T4-A protein protein human Homo sapiens Human MNSLSEANTKFMFDLFQQFRKSKENNIFYSPISITSALGMVLLGAKDNTAQQIKKVLHFDQVTENTTGKAATYHVDRSGNVHHQFQKLLTEFNKSTDAYELKIANKLFGEKTYLFLQEYLDAIKKFYQTSVESVDFANAPEESRKKINSWVESQTNEKIKNLIPEGNIGSNTTLVLVNAIYFKGQWEKKFNKEDTKEEKFWPNKNTYKSIQMMRQYTSFHFASLEDVQAKVLEIPYKGKDLSMIVLLPNEIDGLQKLEEKLTAEKLMEWTSLQNMRETRVDLHLPRFKVEESYDLKDTLRTMGMVDIFNGDADLSGMTGSRGLVLSGVLHKAFVEVTEEGAEAAAATAVVGFGSSPTSTNEEFHCNHPFLFFIRQNKTNSILFYGRFSSP E5F27F986C752CFA csn6_human COP9 signalosome complex subunit 6 COPS6 CSN6 HVIP JAB1-containing signalosome subunit 6 Vpr-interacting protein MOV34 homolog protein protein human Homo sapiens Human MAAAAAAAAATNGTGGSSGMEVDAAVVPSVMACGVTGSVSVALHPLVILNISDHWIRMRSQEGRPVQVIGALIGKQEGRNIEVMNSFELLSHTVEEKIIIDKEYYYTKEEQFKQVFKELEFLGWYTTGGPPDPSDIHVHKQVCEIIESPLFLKLNPMTKHTDLPVSVFESVIDIINGEATMLFAELTYTLATEEAERIGVDHVARMTATGSGENSTVAEHLIAQHSAIKMLHSRVKLILEYVKASEAGEVPFNHEILREAYALCHCLPVLSTDKFKTDFYDQCNDVGLMAYLGTITKTCNTMNQFVNKFNVLYDRQGIGRRMRGLFF DIP protein Q7L5N1 original sequence version: 63 DIP protein Q7L5N1 original sequence version: 102 DIP protein Q7L5N1 original sequence version: 119 E33CAC6ADF799A8D isg15_mouse Ubiquitin-like protein ISG15 Isg15 G1p2 Ucrp Interferon-induced 15 kDa protein Interferon-induced 17 kDa protein Ubiquitin cross-reactive protein protein protein mouse Mus musculus Mouse MAWDLKVKMLGGNDFLVSVTNSMTVSELKKQIAQKIGVPAFQQRLAHQTAVLQDGLTLSSLGLGPSSTVMLVVQNCSEPLSILVRNERGHSNIYEVFLTQTVDTLKKKVSQREQVHEDQFWLSFEGRPMEDKELLGEYGLKPQCTVIKHLRLRGGGGDQCA In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy mint Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections 9C70D9A507979171 isg15_human Ubiquitin-like protein ISG15 ISG15 G1P2 UCRP Interferon-induced 15 kDa protein Interferon-induced 17 kDa protein Ubiquitin cross-reactive protein protein protein human Homo sapiens Human MGWDLTVKMLAGNEFQVSLSSSMSVSELKAQITQKIGVHAFQQRLAVHPSGVALQDRVPLASQGLGPGSTVLLVVDKCDEPLSILVRNNKGRSSTYEVRLTQTVAHLKQQVSGLEGVQDDLFWLTFEGKPLEDQLPLGEYGLKPLSTVFMNLRLRGGGTEPGGRS DIP protein P05161 original sequence version: 130 DIP protein P05161 original sequence version: 142 DIP protein P05161 original sequence version: 155 DIP protein P05161 original sequence version: 96 DIP protein P05161 original sequence version: 141 DIP protein P05161 original sequence version: 168 DIP protein P05161 original sequence version: 150 B6858A15AB0FFFDE leg1_human Galectin-1 Lectin galactoside-binding soluble 1 Beta-galactoside-binding lectin L-14-I S-Lac lectin 1 Galaptin 14 kDa lectin HPL HBL Putative MAPK-activating protein PM12 LGALS1 Lactose-binding lectin 1 14 kDa laminin-binding protein protein protein human Homo sapiens Human MACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD DIP protein P09382 original sequence version: null DIP protein P09382 original sequence version: 111 2FBB8D7A1FC0F1F9 s10a8_human Protein S100-A8 S100A8 CAGA MRP8 CFAG S100 calcium-binding protein A8 Calgranulin-A Migration inhibitory factor-related protein 8 Cystic fibrosis antigen Leukocyte L1 complex light chain Calprotectin L1L subunit Urinary stone protein band A protein protein human Homo sapiens Human MLTELEKALNSIIDVYHKYSLIKGNFHAVYRDDLKKLLETECPQYIRKKGADVWFKELDINTDGAVNFQEFLILVIKMGVAAHKKSHEESHKE 78F589140B9CE166 def1_human Neutrophil defensin 1 HNP-1 Defensin, alpha 1 DEFA1 DEF1 DEFA2 MRS DEFA1B protein protein human Homo sapiens Human MRTLAILAAILLVALQAQAEPLQARADEVAAAPEQIAADIPEVVVSLAWDESLAPKHPGSRKNMACYCRIPACIAGERRYGTCIYQGRLWAFCC 0E0F8E95737396FA rbx1_human E3 ubiquitin-protein ligase RBX1 RBX1 RNF75 ROC1 Regulator of cullins 1 RING finger protein 75 Protein ZYP RING-box protein 1 E3 ubiquitin-protein transferase RBX1 protein protein human Homo sapiens Human MAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH DIP protein P62877 original sequence version: 120 DIP protein P62877 original sequence version: 115 DIP protein P62877 original sequence version: 78 DIP protein P62877 original sequence version: 113 DIP protein P62877 original sequence version: 75 DIP protein P62877 original sequence version: 86 DIP protein P62877 original sequence version: 136 DIP protein P62877 original sequence version: 126 DIP protein P62877 original sequence version: 90 DIP protein P62877 original sequence version: 114 30FC5ADF66096C0E zinc(2+) zinc(2+) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) grl-0617 GRL-0617 small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) csn5_human COP9 signalosome complex subunit 5 Jun activation domain-binding protein 1 COPS5 CSN5 JAB1 protein protein human Homo sapiens Human MAASGSGMAQKTWELANNMQEAQSIDEIYKYDKKQQQEILAAKPWTKDHHYFKYCKISALALLKMVMHARSGGNLEVMGLMLGKVDGETMIIMDSFALPVEGTETRVNAQAAAYEYMAAYIENAKQVGRLENAIGWYHSHPGYGCWLSGIDVSTQMLNQQFQEPFVAVVIDPTRTISAGKVNLGAFRTYPKGYKPPDEGPSEYQTIPLNKIEDFGVHCKQYYALEVSYFKSSLDRKLLELLWNKYWVNTLSSSSLLTNADYTTGQVFDLSEKLEQSEAQLGRGSFMLGLETHDRKSEDKLAKATRDSCKTTIEAIHGLMSQVIKDKLFNQINIS DIP protein Q92905 original sequence version: 161 DIP protein Q92905 original sequence version: 87 DIP protein Q92905 original sequence version: 132 DIP protein Q92905 original sequence version: 149 DIP protein Q92905 original sequence version: 136 DIP protein Q92905 original sequence version: 101 B5742F4AAD03A1CF ubp2_human Ubiquitin carboxyl-terminal hydrolase 2 Ubiquitin-specific-processing protease 2 Deubiquitinating enzyme 2 41 kDa ubiquitin-specific protease USP2 UBP41 Ubiquitin thioesterase 2 protein protein human Homo sapiens Human MSQLSSTLKRYTESARYTDAHYAKSGYGAYTPSSYGANLAASLLEKEKLGFKPVPTSSFLTRPRTYGPSSLLDYDRGRPLLRPDITGGGKRAESQTRGTERPLGSGLSGGSGFPYGVTNNCLSYLPINAYDQGVTLTQKLDSQSDLARDFSSLRTSDSYRIDPRNLGRSPMLARTRKELCTLQGLYQTASCPEYLVDYLENYGRKGSASQVPSQAPPSRVPEIISPTYRPIGRYTLWETGKGQAPGPSRSSSPGRDGMNSKSAQGLAGLRNLGNTCFMNSILQCLSNTRELRDYCLQRLYMRDLHHGSNAHTALVEEFAKLIQTIWTSSPNDVVSPSEFKTQIQRYAPRFVGYNQQDAQEFLRFLLDGLHNEVNRVTLRPKSNPENLDHLPDDEKGRQMWRKYLEREDSRIGDLFVGQLKSSLTCTDCGYCSTVFDPFWDLSLPIAKRGYPEVTLMDCMRLFTKEDVLDGDEKPTCCRCRGRKRCIKKFSIQRFPKILVLHLKRFSESRIRTSKLTTFVNFPLRDLDLREFASENTNHAVYNLYAVSNHSGTTMGGHYTAYCRSPGTGEWHTFNDSSVTPMSSSQVRTSDAYLLFYELASPPSRM DIP protein O75604 original sequence version: 57 AFF4DA9344D21812 s10a7_human Protein S100-A7 S100A7 PSOR1 S100A7C S100 calcium-binding protein A7 Psoriasin protein protein human Homo sapiens Human MSNTQAERSIIGMIDMFHKYTRRDDKIEKPSLLTMMKENFPNFLSACDKKGTNYLADVFEKKDKNEDKKIDFSEFLSLLGDIATDYHKQSHGAAPCSGGSQ 02C4CE39BF140971 prkdc_human DNA-dependent protein kinase catalytic subunit PRKDC HYRC HYRC1 DNPK1 p460 protein protein human Homo sapiens Human MAGSGAGVRCSLLRLQETLSAADRCGAALAGHQLIRGLGQECVLSSSPAVLALQTSLVFSRDFGLLVFVRKSLNSIEFRECREEILKFLCIFLEKMGQKIAPYSVEIKNTCTSVYTKDRAAKCKIPALDLLIKLLQTFRSSRLMDEFKIGELFSKFYGELALKKKIPDTVLEKVYELLGLLGEVHPSEMINNAENLFRAFLGELKTQMTSAVREPKLPVLAGCLKGLSSLLCNFTKSMEEDPQTSREIFNFVLKAIRPQIDLKRYAVPSAGLRLFALHASQFSTCLLDNYVSLFEVLLKWCAHTNVELKKAALSALESFLKQVSNMVAKNAEMHKNKLQYFMEQFYGIIRNVDSNNKELSIAIRGYGLFAGPCKVINAKDVDFMYVELIQRCKQMFLTQTDTGDDRVYQMPSFLQSVASVLLYLDTVPEVYTPVLEHLVVMQIDSFPQYSPKMQLVCCRAIVKVFLALAAKGPVLRNCISTVVHQGLIRICSKPVVLPKGPESESEDHRASGEVRTGKWKVPTYKDYVDLFRHLLSSDQMMDSILADEAFFSVNSSSESLNHLLYDEFVKSVLKIVEKLDLTLEIQTVGEQENGDEAPGVWMIPTSDPAANLHPAKPKDFSAFINLVEFCREILPEKQAEFFEPWVYSFSYELILQSTRLPLISGFYKLLSITVRNAKKIKYFEGVSPKSLKHSPEDPEKYSCFALFVKFGKEVAVKMKQYKDELLASCLTFLLSLPHNIIELDVRAYVPALQMAFKLGLSYTPLAEVGLNALEEWSIYIDRHVMQPYYKDILPCLDGYLKTSALSDETKNNWEVSALSRAAQKGFNKVVLKHLKKTKNLSSNEAISLEEIRIRVVQMLGSLGGQINKNLLTVTSSDEMMKSYVAWDREKRLSFAVPFREMKPVIFLDVFLPRVTELALTASDRQTKVAACELLHSMVMFMLGKATQMPEGGQGAPPMYQLYKRTFPVLLRLACDVDQVTRQLYEPLVMQLIHWFTNNKKFESQDTVALLEAILDGIVDPVDSTLRDFCGRCIREFLKWSIKQITPQQQEKSPVNTKSLFKRLYSLALHPNAFKRLGASLAFNNIYREFREEESLVEQFVFEALVIYMESLALAHADEKSLGTIQQCCDAIDHLCRIIEKKHVSLNKAKKRRLPRGFPPSASLCLLDLVKWLLAHCGRPQTECRHKSIELFYKFVPLLPGNRSPNLWLKDVLKEEGVSFLINTFEGGGCGQPSGILAQPTLLYLRGPFSLQATLCWLDLLLAALECYNTFIGERTVGALQVLGTEAQSSLLKAVAFFLESIAMHDIIAAEKCFGTGAAGNRTSPQEGERYNYSKCTVVVRIMEFTTTLLNTSPEGWKLLKKDLCNTHLMRVLVQTLCEPASIGFNIGDVQVMAHLPDVCVNLMKALKMSPYKDILETHLREKITAQSIEELCAVNLYGPDAQVDRSRLAAVVSACKQLHRAGLLHNILPSQSTDLHHSVGTELLSLVYKGIAPGDERQCLPSLDLSCKQLASGLLELAFAFGGLCERLVSLLLNPAVLSTASLGSSQGSVIHFSHGEYFYSLFSETINTELLKNLDLAVLELMQSSVDNTKMVSAVLNGMLDQSFRERANQKHQGLKLATTILQHWKKCDSWWAKDSPLETKMAVLALLAKILQIDSSVSFNTSHGSFPEVFTTYISLLADTKLDLHLKGQAVTLLPFFTSLTGGSLEELRRVLEQLIVAHFPMQSREFPPGTPRFNNYVDCMKKFLDALELSQSPMLLELMTEVLCREQQHVMEELFQSSFRRIARRGSCVTQVGLLESVYEMFRKDDPRLSFTRQSFVDRSLLTLLWHCSLDALREFFSTIVVDAIDVLKSRFTKLNESTFDTQITKKMGYYKILDVMYSRLPKDDVHAKESKINQVFHGSCITEGNELTKTLIKLCYDAFTENMAGENQLLERRRLYHCAAYNCAISVICCVFNELKFYQGFLFSEKPEKNLLIFENLIDLKRRYNFPVEVEVPMERKKKYIEIRKEAREAANGDSDGPSYMSSLSYLADSTLSEEMSQFDFSTGVQSYSYSSQDPRPATGRFRRREQRDPTVHDDVLELEMDELNRHECMAPLTALVKHMHRSLGPPQGEEDSVPRDLPSWMKFLHGKLGNPIVPLNIRLFLAKLVINTEEVFRPYAKHWLSPLLQLAASENNGGEGIHYMVVEIVATILSWTGLATPTGVPKDEVLANRLLNFLMKHVFHPKRAVFRHNLEIIKTLVECWKDCLSIPYRLIFEKFSGKDPNSKDNSVGIQLLGIVMANDLPPYDPQCGIQSSEYFQALVNNMSFVRYKEVYAAAAEVLGLILRYVMERKNILEESLCELVAKQLKQHQNTMEDKFIVCLNKVTKSFPPLADRFMNAVFFLLPKFHGVLKTLCLEVVLCRVEGMTELYFQLKSKDFVQVMRHRDDERQKVCLDIIYKMMPKLKPVELRELLNPVVEFVSHPSTTCREQMYNILMWIHDNYRDPESETDNDSQEIFKLAKDVLIQGLIDENPGLQLIIRNFWSHETRLPSNTLDRLLALNSLYSPKIEVHFLSLATNFLLEMTSMSPDYPNPMFEHPLSECEFQEYTIDSDWRFRSTVLTPMFVETQASQGTLQTRTQEGSLSARWPVAGQIRATQQQHDFTLTQTADGRSSFDWLTGSSTDPLVDHTSPSSDSLLFAHKRSERLQRAPLKSVGPDFGKKRLGLPGDEVDNKVKGAAGRTDLLRLRRRFMRDQEKLSLMYARKGVAEQKREKEIKSELKMKQDAQVVLYRSYRHGDLPDIQIKHSSLITPLQAVAQRDPIIAKQLFSSLFSGILKEMDKFKTLSEKNNITQKLLQDFNRFLNTTFSFFPPFVSCIQDISCQHAALLSLDPAAVSAGCLASLQQPVGIRLLEEALLRLLPAELPAKRVRGKARLPPDVLRWVELAKLYRSIGEYDVLRGIFTSEIGTKQITQSALLAEARSDYSEAAKQYDEALNKQDWVDGEPTEAEKDFWELASLDCYNHLAEWKSLEYCSTASIDSENPPDLNKIWSEPFYQETYLPYMIRSKLKLLLQGEADQSLLTFIDKAMHGELQKAILELHYSQELSLLYLLQDDVDRAKYYIQNGIQSFMQNYSSIDVLLHQSRLTKLQSVQALTEIQEFISFISKQGNLSSQVPLKRLLNTWTNRYPDAKMDPMNIWDDIITNRCFFLSKIEEKLTPLPEDNSMNVDQDGDPSDRMEVQEQEEDISSLIRSCKFSMKMKMIDSARKQNNFSLAMKLLKELHKESKTRDDWLVSWVQSYCRLSHCRSRSQGCSEQVLTVLKTVSLLDENNVSSYLSKNILAFRDQNILLGTTYRIIANALSSEPACLAEIEEDKARRILELSGSSSEDSEKVIAGLYQRAFQHLSEAVQAAEEEAQPPSWSCGPAAGVIDAYMTLADFCDQQLRKEEENASVIDSAELQAYPALVVEKMLKALKLNSNEARLKFPRLLQIIERYPEETLSLMTKEISSVPCWQFISWISHMVALLDKDQAVAVQHSVEEITDNYPQAIVYPFIISSESYSFKDTSTGHKNKEFVARIKSKLDQGGVIQDFINALDQLSNPELLFKDWSNDVRAELAKTPVNKKNIEKMYERMYAALGDPKAPGLGAFRRKFIQTFGKEFDKHFGKGGSKLLRMKLSDFNDITNMLLLKMNKDSKPPGNLKECSPWMSDFKVEFLRNELEIPGQYDGRGKPLPEYHVRIAGFDERVTVMASLRRPKRIIIRGHDEREHPFLVKGGEDLRQDQRVEQLFQVMNGILAQDSACSQRALQLRTYSVVPMTSRLGLIEWLENTVTLKDLLLNTMSQEEKAAYLSDPRAPPCEYKDWLTKMSGKHDVGAYMLMYKGANRTETVTSFRKRESKVPADLLKRAFVRMSTSPEAFLALRSHFASSHALICISHWILGIGDRHLNNFMVAMETGGVIGIDFGHAFGSATQFLPVPELMPFRLTRQFINLMLPMKETGLMYSIMVHALRAFRSDPGLLTNTMDVFVKEPSFDWKNFEQKMLKKGGSWIQEINVAEKNWYPRQKICYAKRKLAGANPAVITCDELLLGHEKAPAFRDYVAVARGSKDHNIRAQEPESGLSEETQVKCLMDQATDPNILGRTWEGWEPWM DIP protein P78527 original sequence version: 118 DIP protein P78527-1 original sequence version: 117 DIP protein P78527 original sequence version: 170 DIP protein P78527 original sequence version: 179 DIP protein P78527 original sequence version: 114 DIP protein P78527 original sequence version: 125 AC6E747FEB09F3E5 irf3_human Interferon regulatory factor 3 IRF3 protein protein human Homo sapiens Human MGTPKPRILPWLVSQLDLGQLEGVAWVNKSRTRFRIPWKHGLRQDAQQEDFGIFQAWAEATGAYVPGRDKPDLPTWKRNFRSALNRKEGLRLAEDRSKDPHDPHKIYEFVNSGVGDFSQPDTSPDTNGGGSTSDTQEDILDELLGNMVLAPLPDPGPPSLAVAPEPCPQPLRSPSLDNPTPFPNLGPSENPLKRLLVPGEEWEFEVTAFYRGRQVFQQTISCPEGLRLVGSEVGDRTLPGWPVTLPDPGMSLTDRGVMSYVRHVLSCLGGGLALWRAGQWLWAQRLGHCHTYWAVSEELLPNSGHGPDGEVPKDKEGGVFDLGPFIVDLITFTEGSGRSPRYALWFCVGESWPQDQPWTKRLVMVKVVPTCLRALVEMARVGGASSLENTVDLHISNSHPLSLTSDQYKAYLQDLVEGMDFQGPGES DIP protein Q14653 original sequence version: 96 DIP protein Q14653 original sequence version: 75 DIP protein Q14653 original sequence version: 95 DIP protein Q14653-1 original sequence version: 128 DIP protein Q14653 original sequence version: 119 F536676FA78B0110 hnrpk_human Heterogeneous nuclear ribonucleoprotein K HNRNPK HNRPK Transformation up-regulated nuclear protein protein protein human Homo sapiens Human METEQPEETFPNTETNGEFGKRPAEDMEEEQAFKRSRNTDEMVELRILLQSKNAGAVIGKGGKNIKALRTDYNASVSVPDSSGPERILSISADIETIGEILKKIIPTLEEGLQLPSPTATSQLPLESDAVECLNYQHYKGSDFDCELRLLIHQSLAGGIIGVKGAKIKELRENTQTTIKLFQECCPHSTDRVVLIGGKPDRVVECIKIILDLISESPIKGRAQPYDPNFYDETYDYGGFTMMFDDRRGRPVGFPMRGRGGFDRMPPGRGGRPMPPSRRDYDDMSPRRGPPPPPPGRGGRGGSRARNLPLPPPPPPRGGDLMAYDRRGRPGDRYDGMVGFSADETWDSAIDTWSPSEWQMAYEPQGGSGYDYSYAGGRGSYGDLGGPIITTQVTIPKDLAGSIIGKGGQRIKQIRHESGASIKIDEPLEGSEDRIITITGTQDQIQNAQYLLQNSVKQYSGKFF 0F70EE169B2A064A p0dtd1-pro_0000449621 Papain-like protease nsp3 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV APTKVTFGDDTVIEVQGYKSVNITFELDERIDKVLNEKCSAYTVELGTEVNEFACVVADAVIKTLQPVSELLTPLGIDLDEWSMATYYLFDESGEFKLASHMYCSFYPPDEDEEEGDCEEEEFEPSTQYEYGTEDDYQGKPLEFGATSAALQPEEEQEEDWLDDDSQQTVGQQDGSEDNQTTTIQTIVEVQPQLEMELTPVVQTIEVNSFSGYLKLTDNVYIKNADIVEEAKKVKPTVVVNAANVYLKHGGGVAGALNKATNNAMQVESDDYIATNGPLKVGGSCVLSGHNLAKHCLHVVGPNVNKGEDIQLLKSAYENFNQHEVLLAPLLSAGIFGADPIHSLRVCVDTVRTNVYLAVFDKNLYDKLVSSFLEMKSEKQVEQKIAEIPKEEVKPFITESKPSVEQRKQDDKKIKACVEEVTTTLEETKFLTENLLLYIDINGNLHPDSATLVSDIDITFLKKDAPYIVGDVVQEGVLTAVVIPTKKAGGTTEMLAKALRKVPTDNYITTYPGQGLNGYTVEEAKTVLKKCKSAFYILPSIISNEKQEILGTVSWNLREMLAHAEETRKLMPVCVETKAIVSTIQRKYKGIKIQEGVVDYGARFYFYTSKTTVASLINTLNDLNETLVTMPLGYVTHGLNLEEAARYMRSLKVPATVSVSSPDAVTAYNGYLTSSSKTPEEHFIETISLAGSYKDWSYSGQSTQLGIEFLKRGDKSVYYTSNPTTFHLDGEVITFDNLKTLLSLREVRTIKVFTTVDNINLHTQVVDMSMTYGQQFGPTYLDGADVTKIKPHNSHEGKTFYVLPNDDTLRVEAFEYYHTTDPSFLGRYMSALNHTKKWKYPQVNGLTSIKWADNNCYLATALLTLQQIELKFNPPALQDAYYRARAGEAANFCALILAYCNKTVGELGDVRETMSYLFQHANLDSCKRVLNVVCKTCGQQQTTLKGVEAVMYMGTLSYEQFKKGVQIPCTCGKQATKYLVQQESPFVMMSAPPAQYELKHGTFTCASEYTGNYQCGHYKHITSKETLYCIDGALLTKSSEYKGPITDVFYKENSYTTTIKPVTYKLDGVVCTEIDPKLDNYYKKDNSYFTEQPIDLVPNQPYPNASFDNFKFVCDNIKFADDLNQLTGYKKPASRELKVTFFPDLNGDVVAIDYKHYTPSFKKGAKLLHKPIVWHVNNATNKATYKPNTWCIRCLWSTKPVETSNSFDVLKSEDAQGMDNLACEDLKPVSEEVVENPTIQKDVLECNVKTTEVVGDIILKPANNSLKITEEVGHTDLMAAYVDNSSLTIKKPNELSRVLGLKTLATHGLAAVNSVPWDTIANYAKPFLNKVVSTTTNIVTRCLNRVCTNYMPYFFTLLLQLCTFTRSTNSRIKASMPTTIAKNTVKSVGKFCLEASFNYLKSPNFSKLINIIIWFLLLSVCLGSLIYSTAALGVLMSNLGMPSYCTGYREGYLNSTNVTIATYCTGSIPCSVCLSGLDSLDTYPSLETIQITISSFKWDLTAFGLVAEWFLAYILFTRFFYVLGLAAIMQLFFSYFAVHFISNSWLMWLIINLVQMAPISAMVRMYIFFASFYYVWKSYVHVVDGCNSSTCMMCYKRNRATRVECTTIVNGVRRSFYVYANGGKGFCKLHNWNCVNCDTFCAGSTFISDEVARDLSLQFKRPINPTDQSSYIVDSVTVKNGSIHLYFDKAGQKTYERHSLSHFVNLDNLRANNTKGSLPINVIVFDGKSKCEESSAKSASVYYSQLMCQPILLLDQALVSDVGDSAEVAVKMFDAYVNTFSSTFNVPMEKLKTLVATAEAELAKNVSLDNVLSTFISAARQGFVDSDVETKDVVECLKLSHQSDIEVTGDSCNNYMLTYNKVENMTPRDLGACIDCSARHINAQVAKSHNIALIWNVKDFMSLSEQLRKQIRSAAKKNNLPFKLTCATTRQVVNVVTTKIALKGG 819 2763 taldo_human Transaldolase TALDO1 TAL TALDO TALDOR protein protein human Homo sapiens Human MSSSPVKRQRMESALDQLKQFTTVVADTGDFHAIDEYKPQDATTNPSLILAAAQMPAYQELVEEAIAYGRKLGGSQEDQIKNAIDKLFVLFGAEILKKIPGRVSTEVDARLSFDKDAMVARARRLIELYKEAGISKDRILIKLSSTWEGIQAGKELEEQHGIHCNMTLLFSFAQAVACAEAGVTLISPFVGRILDWHVANTDKKSYEPLEDPGVKSVTKIYNYYKKFSYKTIVMGASFRNTGEIKALAGCDFLTISPKLLGELLQDNAKLVPVLSAKAAQASDLEKIHLDEKSFRWLHNEDQMAVEKLSDGIRKFAADAVKLERMLTERMFNAENGK 8CB4992AEF364E64 ikba_human NF-kappa-B inhibitor alpha NFKBIA IKBA MAD3 NFKBI I-kappa-B-alpha Major histocompatibility complex enhancer-binding protein MAD3 protein protein human Homo sapiens Human MFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTEDELPYDDCVFGGQRLTL DIP protein P25963 original sequence version: 160 DIP protein P25963 original sequence version: 157 DIP protein P25963 original sequence version: 145 DIP protein P25963 original sequence version: 170 DIP protein P25963 original sequence version: 122 DIP protein P25963 original sequence version: 128 DIP protein P25963 original sequence version: 78 DIP protein P25963 original sequence version: 121 DIP protein P25963 original sequence version: 124 088B313226786395 senp8_human Sentrin-specific protease 8 SENP8 DEN1 NEDP1 PRSC2 FKSG8 Sentrin/SUMO-specific protease SENP8 Protease, cysteine 2 NEDD8-specific protease 1 Deneddylase-1 protein protein human Homo sapiens Human MDPVVLSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVSFISPEVTQFIKCTSNPAEIAMFLEPLDLPNKRVVFLAINDNSNQAAGGTHWSLLVYLQDKNSFFHYDSHSRSNSVHAKQVAEKLEAFLGRKGDKLAFVEEKAPAQQNSYDCGMYVICNTEALCQNFFRQQTESLLQLLTPAYITKKRGEWKDLITTLAKK 680D93B85EF6028C leg7_human Galectin-7 LGALS7 PIG1 HKL-14 PI7 p53-induced gene 1 protein LGALS7B protein protein human Homo sapiens Human MSNVPHKSSLPEGIRPGTVLRIRGLVPPNASRFHVNLLCGEEQGSDAALHFNPRLDTSEVVFNSKEQGSWGREERGPGVPFQRGQPFEVLIIASDDGFKAVVGDAQYHHFRHRLPLARVRLVEVGGDVQLDSVRIF F613D3540331C13F spb12_human Serpin B12 SERPINB12 protein protein human Homo sapiens Human MDSLVTANTKFCFDLFQEIGKDDRHKNIFFSPLSLSAALGMVRLGARSDSAHQIDEVLHFNEFSQNESKEPDPCLKSNKQKAGSLNNESGLVSCYFGQLLSKLDRIKTDYTLSIANRLYGEQEFPICQEYLDGVIQFYHTTIESVDFQKNPEKSRQEINFWVECQSQGKIKELFSKDAINAETVLVLVNAVYFKAKWETYFDHENTVDAPFCLNANENKSVKMMTQKGLYRIGFIEEVKAQILEMRYTKGKLSMFVLLPSHSKDNLKGLEELERKITYEKMVAWSSSENMSEESVVLSFPRFTLEDSYDLNSILQDMGITDIFDETRADLTGISPSPNLYLSKIIHKTFVEVDENGTQAAAATGAVVSERSLRSWVEFNANHPFLFFIRHNKTQTILFYGRVCSP FFE12D4C9B7F3DFA csn2_human COP9 signalosome complex subunit 2 JAB1-containing signalosome subunit 2 Thyroid receptor-interacting protein 15 Alien homolog COPS2 CSN2 TRIP15 protein protein human Homo sapiens Human MSDMEDDFMCDDEEDYDLEYSEDSNSEPNVDLENQYYNSKALKEDDPKAALSSFQKVLELEGEKGEWGFKALKQMIKINFKLTNFPEMMNRYKQLLTYIRSAVTRNYSEKSINSILDYISTSKQMDLLQEFYETTLEALKDAKNDRLWFKTNTKLGKLYLEREEYGKLQKILRQLHQSCQTDDGEDDLKKGTQLLEIYALEIQMYTAQKNNKKLKALYEQSLHIKSAIPHPLIMGVIRECGGKMHLREGEFEKAHTDFFEAFKNYDESGSPRRTTCLKYLVLANMLMKSGINPFDSQEAKPYKNDPEILAMTNLVSAYQNNDITEFEKILKTNHSNIMDDPFIREHIEELLRNIRTQVLIKLIKPYTRIHIPFISKELNIDVADVESLLVQCILDNTIHGRIDQVNQLLELDHQKRGGARYTALDKWTNQLNSLNQAVVSKLA DIP protein P61201 original sequence version: 67 DIP protein P61201-1 original sequence version: 106 DIP protein P61201 original sequence version: 122 DIP protein P61201-1 original sequence version: 76 1DB6FA774C13BC59 ubc_human Polyubiquitin-C UBC protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGV B6E7BC06FEE77196 catd_human Cathepsin D CTSD CPSD protein protein human Homo sapiens Human MQPSSLLPLALCLLAAPASALVRIPLHKFTSIRRTMSEVGGSVEDLIAKGPVSKYSQAVPAVTEGPIPEVLKNYMDAQYYGEIGIGTPPQCFTVVFDTGSSNLWVPSIHCKLLDIACWIHHKYNSDKSSTYVKNGTSFDIHYGSGSLSGYLSQDTVSVPCQSASSASALGGVKVERQVFGEATKQPGITFIAAKFDGILGMAYPRISVNNVLPVFDNLMQQKLVDQNIFSFYLSRDPDAQPGGELMLGGTDSKYYKGSLSYLNVTRKAYWQVHLDQVEVASGLTLCKEGCEAIVDTGTSLMVGPVDEVRELQKAIGAVPLIQGEYMIPCEKVSTLPAITLKLGGKGYKLSPEDYTLKVSQAGKTLCLSGFMGMDIPPPSGPLWILGDVFIGRYYTVFDRDNNRVGFAEAARL DIP protein P07339 original sequence version: null DIP protein P07339 original sequence version: 170 903FB8412E0CF0B0 ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG csn1_human COP9 signalosome complex subunit 1 JAB1-containing signalosome subunit 1 G protein pathway suppressor 1 Protein MFH GPS1 COPS1 CSN1 protein protein human Homo sapiens Human MPLPVQVFNLQGAVEPMQIDVDPQEDPQNAPDVNYVVENPSLDLEQYAASYSGLMRIERLQFIADHCPTLRVEALKMALSFVQRTFNVDMYEEIHRKLSEATRSSLRELQNAPDAIPESGVEPPALDTAWVEATRKKALLKLEKLDTDLKNYKGNSIKESIRRGHDDLGDHYLDCGDLSNALKCYSRARDYCTSAKHVINMCLNVIKVSVYLQNWSHVLSYVSKAESTPEIAEQRGERDSQTQAILTKLKCAAGLAELAARKYKQAAKCLLLASFDHCDFPELLSPSNVAIYGGLCALATFDRQELQRNVISSSSFKLFLELEPQVRDIIFKFYESKYASCLKMLDEMKDNLLLDMYLAPHVRTLYTQIRNRALIQYFSPYVSADMHRMAAAFNTTVAALEDELTQLILEGLISARVDSHSKILYARDVDQRSTTFEKSLLMGKEFQRRAKAMMLRAAVLRNQIHVKSPPREGSQGELTPANSQSRMSTNM DIP protein Q13098 original sequence version: 97 DIP protein Q13098 original sequence version: 154 DIP protein Q13098 original sequence version: 134 BF925164ED985638 grp75_human Stress-70 protein, mitochondrial HSPA9 GRP75 HSPA9B 75 kDa glucose-regulated protein Heat shock 70 kDa protein 9 Peptide-binding protein 74 Mortalin mt-HSP70 protein protein human Homo sapiens Human MISASRAAAARLVGAAASRGPTAARHQDSWNGLSHEAFRLVSRRDYASEAIKGAVVGIDLGTTNSCVAVMEGKQAKVLENAEGARTTPSVVAFTADGERLVGMPAKRQAVTNPNNTFYATKRLIGRRYDDPEVQKDIKNVPFKIVRASNGDAWVEAHGKLYSPSQIGAFVLMKMKETAENYLGHTAKNAVITVPAYFNDSQRQATKDAGQISGLNVLRVINEPTAAALAYGLDKSEDKVIAVYDLGGGTFDISILEIQKGVFEVKSTNGDTFLGGEDFDQALLRHIVKEFKRETGVDLTKDNMALQRVREAAEKAKCELSSSVQTDINLPYLTMDSSGPKHLNMKLTRAQFEGIVTDLIRRTIAPCQKAMQDAEVSKSDIGEVILVGGMTRMPKVQQTVQDLFGRAPSKAVNPDEAVAIGAAIQGGVLAGDVTDVLLLDVTPLSLGIETLGGVFTKLINRNTTIPTKKSQVFSTAADGQTQVEIKVCQGEREMAGDNKLLGQFTLIGIPPAPRGVPQIEVTFDIDANGIVHVSAKDKGTGREQQIVIQSSGGLSKDDIENMVKNAEKYAEEDRRKKERVEAVNMAEGIIHDTETKMEEFKDQLPADECNKLKEEISKMRELLARKDSETGENIRQAASSLQQASLKLFEMAYKKMASEREGSGSSGTGEQKEDQKEEKQ DIP protein P38646 original sequence version: 188 90969A8D06757753 csn4_human COP9 signalosome complex subunit 4 COPS4 CSN4 JAB1-containing signalosome subunit 4 protein protein human Homo sapiens Human MAAAVRQDLAQLMNSSGSHKDLAGKYRQILEKAIQLSGAEQLEALKAFVEAMVNENVSLVISRQLLTDFCTHLPNLPDSTAKEIYHFTLEKIQPRVISFEEQVASIRQHLASIYEKEEDWRNAAQVLVGIPLETGQKQYNVDYKLETYLKIARLYLEDDDPVQAEAYINRASLLQNESTNEQLQIHYKVCYARVLDYRRKFIEAAQRYNELSYKTIVHESERLEALKHALHCTILASAGQQRSRMLATLFKDERCQQLAAYGILEKMYLDRIIRGNQLQEFAAMLMPHQKATTADGSSILDRAVIEHNLLSASKLYNNITFEELGALLEIPAAKAEKIASQMITEGRMNGFIDQIDGIVHFETREALPTWDKQIQSLCFQVNNLLEKISQTAPEWTAQAMEAQMAQ DIP protein Q9BT78 original sequence version: 72 DIP protein Q9BT78-1 original sequence version: 115 DIP protein Q9BT78 original sequence version: 132 DIP protein Q9BT78 original sequence version: 110 4EFF9079058EB609 csn3_human COP9 signalosome complex subunit 3 COPS3 CSN3 JAB1-containing signalosome subunit 3 protein protein human Homo sapiens Human MASALEQFVNSVRQLSAQGQMTQLCELINKSGELLAKNLSHLDTVLGALDVQEHSLGVLAVLFVKFSMPSVPDFETLFSQVQLFISTCNGEHIRYATDTFAGLCHQLTNALVERKQPLRGIGILKQAIDKMQMNTNQLTSIHADLCQLCLLAKCFKPALPYLDVDMMDICKENGAYDAKHFLCYYYYGGMIYTGLKNFERALYFYEQAITTPAMAVSHIMLESYKKYILVSLILLGKVQQLPKYTSQIVGRFIKPLSNAYHELAQVYSTNNPSELRNLVNKHSETFTRDNNMGLVKQCLSSLYKKNIQRLTKTFLTLSLQDMASRVQLSGPQEAEKYVLHMIEDGEIFASINQKDGMVSFHDNPEKYNNPAMLHNIDQEMLKCIELDERLKAMDQEITVNPQFVQKSMGSQEDDSGNKPSSYS DIP protein Q9UNS2 original sequence version: 82 DIP protein Q9UNS2-1 original sequence version: 122 DIP protein Q9UNS2 original sequence version: 140 1D371050C7D7BF8D p0c6x7-pro_0000037311 Papain-like protease nsp3 rep ORF1ab polyprotein 1a-1b protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus APIKGVTFGEDTVWEVQGYKNVRITFELDERVDKVLNEKCSVYTVESGTEVTEFACVVAEAVVKTLQPVSDLLTNMGIDLDEWSVATFYLFDDAGEENFSSRMYCSFYPPDEEEEDDAECEEEEIDETCEHEYGTEDDYQGLPLEFGASAETVRVEEEEEEDWLDDTTEQSEIEPEPEPTPEEPVNQFTGYLKLTDNVAIKCVDIVKEAQSANPMVIVNAANIHLKHGGGVAGALNKATNGAMQKESDDYIKLNGPLTVGGSCLLSGHNLAKKCLHVVGPNLNAGEDIQLLKAAYENFNSQDILLAPLLSAGIFGAKPLQSLQVCVQTVRTQVYIAVNDKALYEQVVMDYLDNLKPRVEAPKQEEPPNTEDSKTEEKSVVQKPVDVKPKIKACIDEVTTTLEETKFLTNKLLLFADINGKLYHDSQNMLRGEDMSFLEKDAPYMVGDVITSGDITCVVIPSKKAGGTTEMLSRALKKVPVDEYITTYPGQGCAGYTLEEAKTALKKCKSAFYVLPSEAPNAKEEILGTVSWNLREMLAHAEETRKLMPICMDVRAIMATIQRKYKGIKIQEGIVDYGVRFFFYTSKEPVASIITKLNSLNEPLVTMPIGYVTHGFNLEEAARCMRSLKAPAVVSVSSPDAVTTYNGYLTSSSKTSEEHFVETVSLAGSYRDWSYSGQRTELGVEFLKRGDKIVYHTLESPVEFHLDGEVLSLDKLKSLLSLREVKTIKVFTTVDNTNLHTQLVDMSMTYGQQFGPTYLDGADVTKIKPHVNHEGKTFFVLPSDDTLRSEAFEYYHTLDESFLGRYMSALNHTKKWKFPQVGGLTSIKWADNNCYLSSVLLALQQLEVKFNAPALQEAYYRARAGDAANFCALILAYSNKTVGELGDVRETMTHLLQHANLESAKRVLNVVCKHCGQKTTTLTGVEAVMYMGTLSYDNLKTGVSIPCVCGRDATQYLVQQESSFVMMSAPPAEYKLQQGTFLCANEYTGNYQCGHYTHITAKETLYRIDGAHLTKMSEYKGPVTDVFYKETSYTTTIKPVSYKLDGVTYTEIEPKLDGYYKKDNAYYTEQPIDLVPTQPLPNASFDNFKLTCSNTKFADDLNQMTGFTKPASRELSVTFFPDLNGDVVAIDYRHYSASFKKGAKLLHKPIVWHINQATTKTTFKPNTWCLRCLWSTKPVDTSNSFEVLAVEDTQGMDNLACESQQPTSEEVVENPTIQKEVIECDVKTTEVVGNVILKPSDEGVKVTQELGHEDLMAAYVENTSITIKKPNELSLALGLKTIATHGIAAINSVPWSKILAYVKPFLGQAAITTSNCAKRLAQRVFNNYMPYVFTLLFQLCTFTKSTNSRIRASLPTTIAKNSVKSVAKLCLDAGINYVKSPKFSKLFTIAMWLLLLSICLGSLICVTAAFGVLLSNFGAPSYCNGVRELYLNSSNVTTMDFCEGSFPCSICLSGLDSLDSYPALETIQVTISSYKLDLTILGLAAEWVLAYMLFTKFFYLLGLSAIMQVFFGYFASHFISNSWLMWFIISIVQMAPVSAMVRMYIFFASFYYIWKSYVHIMDGCTSSTCMMCYKRNRATRVECTTIVNGMKRSFYVYANGGRGFCKTHNWNCLNCDTFCTGSTFISDEVARDLSLQFKRPINPTDQSSYIVDSVAVKNGALHLYFDKAGQKTYERHPLSHFVNLDNLRANNTKGSLPINVIVFDGKSKCDESASKSASVYYSQLMCQPILLLDQALVSDVGDSTEVSVKMFDAYVDTFSATFSVPMEKLKALVATAHSELAKGVALDGVLSTFVSAARQGVVDTDVDTKDVIECLKLSHHSDLEVTGDSCNNFMLTYNKVENMTPRDLGACIDCNARHINAQVAKSHNVSLIWNVKDYMSLSEQLRKQIRSAAKKNNIPFRLTCATTRQVVNVITTKISLKGG 819 2740 BBB1A6190358ADDE csn8_human COP9 signalosome complex subunit 8 COPS8 CSN8 JAB1-containing signalosome subunit 8 COP9 homolog protein protein human Homo sapiens Human MPVAVMAESAFSFKKLLDQCENQELEAPGGIATPPVYGQLLALYLLHNDMNNARYLWKRIPPAIKSANSELGGIWSVGQRIWQRDFPGIYTTINAHQWSETVQPIMEALRDATRRRAFALVSQAYTSIIADDFAAFVGLPVEEAVKGILEQGWQADSTTRMVLPRKPVAGALDVSFNKFIPLSEPAPVPPIPNEQQLARLTDYVAFLEN DIP protein Q99627 original sequence version: 79 DIP protein Q99627-1 original sequence version: 118 DIP protein Q99627 original sequence version: 135 203E236D8E304A8A rab1a_human Ras-related protein Rab-1A YPT1-related protein RAB1A RAB1 protein protein human Homo sapiens Human MSSMNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKLLVGNKCDLTTKKVVDYTTAKEFADSLGIPFLETSAKNATNVEQSFMTMAAEIKKRMGPGATAGGAEKSNVKIQSTPVKQSGGGCC B2A8F4E3B0FB17D6 phb2_human Prohibitin-2 BAP REA PHB2 D-prohibitin B-cell receptor-associated protein BAP37 Repressor of estrogen receptor activity protein protein human Homo sapiens Human MAQNLKDLAGRLPAGPRGMGTALKLLLGAGAVAYGVRESVFTVEGGHRAIFFNRIGGVQQDTILAEGLHFRIPWFQYPIIYDIRARPRKISSPTGSKDLQMVNISLRVLSRPNAQELPSMYQRLGLDYEERVLPSIVNEVLKSVVAKFNASQLITQRAQVSLLIRRELTERAKDFSLILDDVAITELSFSREYTAAVEAKQVAQQEAQRAQFLVEKAKQEQRQKIVQAEGEAEAAKMLGEALSKNPGYIKLRKIRAAQNISKTIATSQNRIYLTADNLVLNLQDESFTRGSDSLIKGKK A887CC982BF85C80 rep-ubc-2 2 33 weight silver stain molecular weight estimation by silver staining weight silver stain weight silver stain molecular weight estimation by silver staining weight silver stain unspecified role unspecified role neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 40 unspecified role unspecified role neutral component neutral component purified purified propargylamide modification modres protein modification ModRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Lys48 ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain covalent binding covalent binding 1A (RHS) weak activity rep-ubc-3 2 19 enzyme target enzyme target substrate substrate neutral component neutral component purified purified propargylamide modification modres protein modification ModRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 33 unspecified role unspecified role neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0DTD1-PRO_0000449621:p.Phe814Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F A P0DTD1-PRO_0000449621:p.Val811Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain V A ecoli Escherichia coli (strain K12) covalent binding covalent binding 1A (RHS),2F, Ext. Data 1F, figure 4H rep-ubc-4 5 47 enzyme enzyme neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 40 enzyme target enzyme target substrate substrate neutral component neutral component purified purified Lys48 ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain 7-amido-4-methylcoumarine coumarin label coumarin label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cleavage cleavage reaction cleavage 1B/C, 2D, Ext. Data tables 2+3, figure 4D isg15-rep-4 5 19 enzyme target enzyme target substrate substrate neutral component neutral component purified purified 7-amido-4-methylcoumarine coumarin label coumarin label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 33 enzyme enzyme neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0DTD1-PRO_0000449621:p.Thr820Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain T A Mon Mar 01 11:44:30 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p0dtd1-pro_0000449621:p.Thr820Ala' P0DTD1-PRO_0000449621:p.Thr820Leu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain T L Mon Mar 01 11:44:22 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p0dtd1-pro_0000449621:p.Thr820Leu' ecoli Escherichia coli (strain K12) cleavage cleavage reaction cleavage 1B/C, 2D, 3C, Ext. Data tables 2+3, Ext. Data Figure 1G rep-ubc-5 2 26 inhibitor inhibitor neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 47 unspecified role unspecified role neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 40 unspecified role unspecified role neutral component neutral component purified purified propargylamide modification modres protein modification ModRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Lys48 ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain covalent binding covalent binding 1A (LHS), Ext Data figure 4G rep-ubc-6 2 19 unspecified role unspecified role neutral component neutral component purified purified propargylamide modification modres protein modification ModRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 47 unspecified role unspecified role neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) covalent binding covalent binding 1A (LHS) isg15-rep-6 5 33 enzyme enzyme neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0DTD1-PRO_0000449621:p.Thr820Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain T A Mon Mar 01 11:34:20 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p0dtd1-pro_0000449621:Thr820Ala' P0DTD1-PRO_0000449621:p.Thr820Leu mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain T L Mon Mar 01 10:51:25 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p0dtd1-pro_0000449621:Thr820Leu' ecoli Escherichia coli (strain K12) 40 enzyme target enzyme target substrate substrate neutral component neutral component purified purified Lys48 ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain 7-amido-4-methylcoumarine coumarin label coumarin label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cleavage cleavage reaction cleavage 1B/C, 2D, Ext. Data tables 2+3, figure 4D isg15-rep-7 5 19 enzyme target enzyme target substrate substrate neutral component neutral component purified purified 7-amido-4-methylcoumarine coumarin label coumarin label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 47 enzyme enzyme neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) cleavage cleavage reaction cleavage 1B/C,2D, Ext. Data tables 2+3, figure 4F isg15-rep-8 5 33 enzyme enzyme neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 20 enzyme target enzyme target substrate substrate neutral component neutral component purified purified 7-amido-4-methylcoumarine coumarin label coumarin label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cleavage cleavage reaction cleavage 1C Ext. Data table 3 isg15-rep-9 5 47 enzyme enzyme neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 20 enzyme target enzyme target substrate substrate neutral component neutral component purified purified 7-amido-4-methylcoumarine coumarin label coumarin label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain cleavage cleavage reaction cleavage 1C, Ext. Data table 3 isg15-rep-10 4 19 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 33 enzyme enzyme neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0DTD1-PRO_0000449621:p.Cys856Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Mon Mar 01 11:13:21 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p0dtd1-pro_0000449621:p.Cys856Ser' ecoli Escherichia coli (strain K12) direct interaction direct interaction Ext. data Table 1 isg15-rep-11 5 19 enzyme target enzyme target substrate substrate neutral component neutral component purified purified 7-amido-4-methylcoumarine coumarin label coumarin label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 26 inhibitor inhibitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) 33 enzyme enzyme neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range p0dtd1-pro_0000449621:p.Tyr1013Thr mutation with complex effect mutation with complex effect certain certain sequence position certain certain certain sequence position certain Y T ecoli Escherichia coli (strain K12) cleavage cleavage reaction cleavage 3C The mutation of Tyr268 to either Thr (Y269T) or Gly (Y268G) in SCoV2-PLpro strongly reduced the inhibitory effect of GRL-0617. rep-hnrnpk-1 3 49 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 20 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 50 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 34 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 44 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 21 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 32 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 30 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 33 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0DTD1-PRO_0000449621:p.Cys856Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Mon Mar 01 11:38:24 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p0dtd1-pro_0000449621:p.Cys856Ser' n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association 4A rep-hnrnpk-2 3 29 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 22 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 47 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P0C6X7-PRO_0000037311:p.Cys833Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Mon Mar 01 11:15:45 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p0c6x7-pro_0000037311:p.Cys833Ser' 23 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 41 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 37 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 42 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 38 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 11 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 17 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 14 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level association association 4A rep-isg15-1 6 20 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 33 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0DTD1-PRO_0000449621:p.Cys856Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Mon Mar 01 10:43:31 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p0dtd1-pro_0000449621:p.Cys856Ser' n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 4C GRL-0617 (CHEBI:167176) Unconjugated ISG15 and ISG15-positive smears probably representing ISGylated substrates were seen. rep-isg15-2 6 47 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P0C6X7-PRO_0000037311:p.Cys833Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Mon Mar 01 11:06:47 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p0c6x7-pro_0000037311:p.Cys833Ser' 20 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association 4C GRL-0617 (CHEBI:167176) Weak interaction. Unconjugated ISG15 and ISG15-positive smears probably representing ISGylated substrates were seen. irf3-isg15-1 1 31 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level isgylated lysine lys L-lysine residue (2S)-2,6-diaminohexanoic acid 2,6-bis(azanyl)hexanoic acid 6-amino-L-norleucine ACT_SITE Schiff-base intermediate with substrate L-lysine alpha,epsilon-diaminocaproic acid Lys undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined observed-ptm observed-ptm observed ptm observed-ptm 20 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 31 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association 5E GRL-0617, (CHEBI:167176) in SARS-CoV-2-infected cells, as well as SARS infected cells. isg15-rep-12 2 19 unspecified role unspecified role neutral component neutral component purified purified propargylamide modification modres protein modification ModRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 40 competitor competitor competitor unspecified role unspecified role purified purified 47 unspecified role unspecified role neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) covalent binding covalent binding Ext. Data 1E isg15-rep-13 5 19 enzyme target enzyme target substrate substrate neutral component neutral component purified purified 7-amido-4-methylcoumarine coumarin label coumarin label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 40 competitor competitor competitor unspecified role unspecified role purified purified Lys48 ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain 47 enzyme enzyme neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) cleavage cleavage reaction cleavage Ext. Data figure 1E, isg15-rep-14 2 19 enzyme target enzyme target substrate substrate neutral component neutral component purified purified propargylamide modification modres protein modification ModRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role neutral component neutral component purified purified covalent binding covalent binding Ext. Data 1F isg15-rep-15 5 19 enzyme target enzyme target substrate substrate neutral component neutral component purified purified 7-amido-4-methylcoumarine coumarin label coumarin label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 12 enzyme enzyme neutral component neutral component purified purified cleavage cleavage reaction cleavage Ext. Data Figure 1G,Ext. Data table 2 cul1-nedd8-6 8 13 enzyme target enzyme target substrate substrate neutral component neutral component purified purified lys720 xlnk-n6lys-1gly N6-glycyl-L-lysine (2S)-2-amino-6-[(aminoacetyl)amino]hexanoic acid N6-(glycyl)-L-lysine N6-glycyllysine XLNK-N6Lys-1Gly CROSSLNK Glycyl lysine isopeptide (Gly-Lys) (interchain with K-...) CROSSLNK Glycyl lysine isopeptide (Lys-Gly) (interchain with G-...) certain certain sequence position certain certain certain sequence position certain trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 36 enzyme enzyme neutral component neutral component purified purified 15 enzyme target enzyme target substrate substrate neutral component neutral component purified purified Gly76 xlnk-n6lys-1gly N6-glycyl-L-lysine (2S)-2-amino-6-[(aminoacetyl)amino]hexanoic acid N6-(glycyl)-L-lysine N6-glycyllysine XLNK-N6Lys-1Gly CROSSLNK Glycyl lysine isopeptide (Gly-Lys) (interchain with K-...) CROSSLNK Glycyl lysine isopeptide (Lys-Gly) (interchain with G-...) certain certain sequence position certain certain certain sequence position certain trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 24 ancillary ancillary neutral component neutral component purified purified trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 219 216 cleavage cleavage reaction cleavage Ext. Data 1I I can see NO complexes in CP for RBX1/CUL1. NEDD8 need not be entered as an interactor - it could be a "neddylated lysine" as a Feature on CUL1. cop9_human_2-cul1-1 8 13 enzyme target enzyme target substrate substrate neutral component neutral component purified purified lys720 xlnk-n6lys-1gly N6-glycyl-L-lysine (2S)-2-amino-6-[(aminoacetyl)amino]hexanoic acid N6-(glycyl)-L-lysine N6-glycyllysine XLNK-N6Lys-1Gly CROSSLNK Glycyl lysine isopeptide (Gly-Lys) (interchain with K-...) CROSSLNK Glycyl lysine isopeptide (Lys-Gly) (interchain with G-...) certain certain sequence position certain certain certain sequence position certain trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 225 enzyme enzyme neutral component neutral component purified purified 15 enzyme target enzyme target substrate substrate neutral component neutral component purified purified Gly76 xlnk-n6lys-1gly N6-glycyl-L-lysine (2S)-2-amino-6-[(aminoacetyl)amino]hexanoic acid N6-(glycyl)-L-lysine N6-glycyllysine XLNK-N6Lys-1Gly CROSSLNK Glycyl lysine isopeptide (Gly-Lys) (interchain with K-...) CROSSLNK Glycyl lysine isopeptide (Lys-Gly) (interchain with G-...) certain certain sequence position certain certain certain sequence position certain trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 24 ancillary ancillary neutral component neutral component purified purified trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 227 223 cleavage cleavage reaction cleavage Ext. Data 1I I can see NO complexes in CP for RBX1/CUL1. NEDD8 need not be entered as an interactor - it could be a "neddylated lysine" as a Feature on CUL1. The expression system for CSN is unclear. cul1-nedd8-7 8 13 enzyme target enzyme target substrate substrate neutral component neutral component purified purified lys720 xlnk-n6lys-1gly N6-glycyl-L-lysine (2S)-2-amino-6-[(aminoacetyl)amino]hexanoic acid N6-(glycyl)-L-lysine N6-glycyllysine XLNK-N6Lys-1Gly CROSSLNK Glycyl lysine isopeptide (Gly-Lys) (interchain with K-...) CROSSLNK Glycyl lysine isopeptide (Lys-Gly) (interchain with G-...) certain certain sequence position certain certain certain sequence position certain trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 28 enzyme enzyme neutral component neutral component purified purified 15 enzyme target enzyme target substrate substrate neutral component neutral component purified purified Gly76 xlnk-n6lys-1gly N6-glycyl-L-lysine (2S)-2-amino-6-[(aminoacetyl)amino]hexanoic acid N6-(glycyl)-L-lysine N6-glycyllysine XLNK-N6Lys-1Gly CROSSLNK Glycyl lysine isopeptide (Gly-Lys) (interchain with K-...) CROSSLNK Glycyl lysine isopeptide (Lys-Gly) (interchain with G-...) certain certain sequence position certain certain certain sequence position certain trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 24 ancillary ancillary neutral component neutral component purified purified trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 234 231 cleavage cleavage reaction cleavage Ext. Data 1I I can see NO complexes in CP for RBX1/CUL1. NEDD8 need not be entered as an interactor - it could be a "neddylated lysine" as a Feature on CUL1. cul1-nedd8-8 8 13 enzyme target enzyme target substrate substrate neutral component neutral component purified purified lys720 xlnk-n6lys-1gly N6-glycyl-L-lysine (2S)-2-amino-6-[(aminoacetyl)amino]hexanoic acid N6-(glycyl)-L-lysine N6-glycyllysine XLNK-N6Lys-1Gly CROSSLNK Glycyl lysine isopeptide (Gly-Lys) (interchain with K-...) CROSSLNK Glycyl lysine isopeptide (Lys-Gly) (interchain with G-...) certain certain sequence position certain certain certain sequence position certain trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 33 enzyme enzyme neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0DTD1-PRO_0000449621:p.Cys856Ser mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain C S Mon Mar 01 11:39:00 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p0dtd1-pro_0000449621:p.Cys 856Ser' ecoli Escherichia coli (strain K12) 15 enzyme target enzyme target substrate substrate neutral component neutral component purified purified Gly76 xlnk-n6lys-1gly N6-glycyl-L-lysine (2S)-2-amino-6-[(aminoacetyl)amino]hexanoic acid N6-(glycyl)-L-lysine N6-glycyllysine XLNK-N6Lys-1Gly CROSSLNK Glycyl lysine isopeptide (Gly-Lys) (interchain with K-...) CROSSLNK Glycyl lysine isopeptide (Lys-Gly) (interchain with G-...) certain certain sequence position certain certain certain sequence position certain trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 24 ancillary ancillary neutral component neutral component purified purified trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 238 244 cleavage cleavage reaction cleavage Ext. Data 1I I can see NO complexes in CP for RBX1/CUL1. NEDD8 need not be entered as an interactor - it could be a "neddylated lysine" as a Feature on CUL1. cul1-nedd8-9 8 13 enzyme target enzyme target substrate substrate neutral component neutral component purified purified lys720 xlnk-n6lys-1gly N6-glycyl-L-lysine (2S)-2-amino-6-[(aminoacetyl)amino]hexanoic acid N6-(glycyl)-L-lysine N6-glycyllysine XLNK-N6Lys-1Gly CROSSLNK Glycyl lysine isopeptide (Gly-Lys) (interchain with K-...) CROSSLNK Glycyl lysine isopeptide (Lys-Gly) (interchain with G-...) certain certain sequence position certain certain certain sequence position certain trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 47 enzyme enzyme neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0C6X7-PRO_0000037311:p.Cys833Ser mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain C S Mon Mar 01 11:21:08 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p0c6x7-pro_0000037311:p.Cys833Ser' ecoli Escherichia coli (strain K12) 15 enzyme target enzyme target substrate substrate neutral component neutral component purified purified Gly76 xlnk-n6lys-1gly N6-glycyl-L-lysine (2S)-2-amino-6-[(aminoacetyl)amino]hexanoic acid N6-(glycyl)-L-lysine N6-glycyllysine XLNK-N6Lys-1Gly CROSSLNK Glycyl lysine isopeptide (Gly-Lys) (interchain with K-...) CROSSLNK Glycyl lysine isopeptide (Lys-Gly) (interchain with G-...) certain certain sequence position certain certain certain sequence position certain trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 24 ancillary ancillary neutral component neutral component purified purified trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 248 254 cleavage cleavage reaction cleavage Ext. Data 1I I can see NO complexes in CP for RBX1/CUL1. NEDD8 need not be entered as an interactor - it could be a "neddylated lysine" as a Feature on CUL1. rep-ubc-7 5 26 cofactor cofactor coenzyme neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 33 enzyme enzyme neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range p0dtd1-pro_0000449621:Tyr1013Gly mutation with complex effect mutation with complex effect certain certain sequence position certain certain certain sequence position certain Y G ecoli Escherichia coli (strain K12) 40 enzyme target enzyme target substrate substrate neutral component neutral component purified purified 7-amido-4-methylcoumarine coumarin label coumarin label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Lys48 ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain cleavage cleavage reaction cleavage Ext.Data figure 4D The mutation of Tyr268 to either Thr (Y269T) or Gly (Y268G) in SCoV2-PLpro strongly reduced the inhibitory effect of GRL-0617. rep-ubc-8 5 26 inhibitor inhibitor neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 47 enzyme enzyme neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 40 enzyme target enzyme target substrate substrate neutral component neutral component purified purified Lys48 ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain 7-amido-4-methylcoumarine coumarin label coumarin label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cleavage cleavage reaction cleavage Ext. Data figure 4D rep-ubc-9 5 10 enzyme enzyme neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 40 enzyme target enzyme target substrate substrate neutral component neutral component purified purified Lys48 ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain 7-amido-4-methylcoumarine coumarin label coumarin label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cleavage cleavage reaction cleavage Ext. Data figure 4D grl-0617 (CHEBI:167176 ) has no inhibitory effect. isg15-rep-16 5 19 enzyme target enzyme target substrate substrate neutral component neutral component purified purified 7-amido-4-methylcoumarine coumarin label coumarin label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 26 inhibitor inhibitor neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 47 enzyme enzyme neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) cleavage cleavage reaction cleavage Ext. Data figure 4F rep-ubc-10 5 19 enzyme target enzyme target substrate substrate neutral component neutral component 7-amido-4-methylcoumarine coumarin label coumarin label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 10 enzyme enzyme neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) cleavage cleavage reaction cleavage Ext. Data figure 4F grl-0617 (CHEBI:167176 ) has no inhibitory effect. rep-ubc-11 2 40 unspecified role unspecified role neutral component neutral component purified purified Lys48 ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain propargylamide modification modres protein modification ModRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 47 unspecified role unspecified role neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) covalent binding covalent binding Ext Data figure 4G grl_0617-rep-1 2 26 inhibitor inhibitor neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 47 unspecified role unspecified role neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 40 unspecified role unspecified role neutral component neutral component purified purified propargylamide modification modres protein modification ModRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined covalent binding covalent binding 1A (LHS), Ext Data figure 4G grl_0617-rep-2 2 47 unspecified role unspecified role neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 40 unspecified role unspecified role neutral component neutral component purified purified propargylamide modification modres protein modification ModRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined covalent binding covalent binding Ext Data figure 4G isg15-rep-17 2 19 enzyme target enzyme target substrate substrate neutral component neutral component purified purified propargylamide modification modres protein modification ModRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 26 inhibitor inhibitor neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 33 unspecified role unspecified role neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) covalent binding covalent binding Ext. Data figure 4H The effects of GRL-0617 on the reaction between Prg probes with SCoV2-PLpro were more prominent with ISG15C-term-Prg than ISG15FL-Prg. nfkbia-rep-1 5 33 enzyme enzyme neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 35 enzyme target enzyme target substrate substrate neutral component neutral component purified purified region fluorophore fluorophore undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined lys21_22 ubiquitinylated lysine ubiquitinylated lysine range range range range cleavage cleavage reaction cleavage Ext. data figure 5G nfkbia-rep-2 5 47 enzyme enzyme neutral component neutral component purified purified UBL_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 35 enzyme target enzyme target substrate substrate neutral component neutral component purified purified region fluorophore fluorophore undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined lys21_22 ubiquitinylated lysine ubiquitinylated lysine range range range range cleavage cleavage reaction cleavage Ext. data figure 5G rep-isg15-3 9 31 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 20 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association 4D SCoV2-PLpro and SCoV-PLpro reduced IRF3 ISGylation; active site mutants (C111S) did not. cop9_human-2 COP9 signalosome variant 2 COP9 signalosome variant 2 Signalosome CSN complex Cop9 complex COPS2:COPS3:COPS4:COPS5:COPS6:COPS7B:COPS8:GPS1 25 cofactor cofactor coenzyme cops5 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain 16 unspecified role unspecified role 27 unspecified role unspecified role zinc binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 45 unspecified role unspecified role 48 unspecified role unspecified role 46 unspecified role unspecified role 43 unspecified role unspecified role 39 unspecified role unspecified role 18 unspecified role unspecified role 342 345 physical association physical association human Homo sapiens Human stable complex stable complex biological system reconstruction evidence based on paralogy evidence used in manual assertion biological system reconstruction evidence based on paralogy evidence used in manual assertion Essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. created with intact-jami-dbscripts