IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Interaction between IDH1 WT and calmodulin and its implications for glioblastoma cell growth and migration. Interaction between IDH1 WT and calmodulin and its implications for glioblastoma cell growth and migration. Biochemical and biophysical research communications 2020 imex curation Kang S., Kwon HN., Kang S., Park S. Cancer - Interactions investigated in the context of cancer Rare diseases - Interactions investigated in the context of Rare genetic disease lovesmart@snu.ac.kr,psh@snu.ac.kr 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-u87 Human U-87 MG glioblastoma cell line u87 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kang S., Kwon HN., Kang S., Park S. Rare diseases - Interactions investigated in the context of Rare genetic disease lovesmart@snu.ac.kr,psh@snu.ac.kr Accepted 2021-APR-12 AT 10:24 BST AT 10:24 BST by MGT Biochemical and biophysical research communications 2020 Only protein-protein interactions imex curation imex curation Interaction between IDH1 WT and calmodulin and its implications for glioblastoma cell growth and migration. Interaction between IDH1 WT and calmodulin and its implications for glioblastoma cell growth and migration. Biochemical and biophysical research communications 2020 imex curation Kang S., Kwon HN., Kang S., Park S. Cancer - Interactions investigated in the context of cancer Rare diseases - Interactions investigated in the context of Rare genetic disease lovesmart@snu.ac.kr,psh@snu.ac.kr 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie NaCl (CHEBI:26710) molar molar M M 150 mM 0 mN Kang S., Kwon HN., Kang S., Park S. Rare diseases - Interactions investigated in the context of Rare genetic disease lovesmart@snu.ac.kr,psh@snu.ac.kr Accepted 2021-APR-12 AT 10:24 BST AT 10:24 BST by MGT Biochemical and biophysical research communications 2020 Only protein-protein interactions imex curation imex curation Interaction between IDH1 WT and calmodulin and its implications for glioblastoma cell growth and migration. Interaction between IDH1 WT and calmodulin and its implications for glioblastoma cell growth and migration. Biochemical and biophysical research communications 2020 imex curation Kang S., Kwon HN., Kang S., Park S. Cancer - Interactions investigated in the context of cancer Rare diseases - Interactions investigated in the context of Rare genetic disease lovesmart@snu.ac.kr,psh@snu.ac.kr 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Kang S., Kwon HN., Kang S., Park S. Rare diseases - Interactions investigated in the context of Rare genetic disease lovesmart@snu.ac.kr,psh@snu.ac.kr Accepted 2021-APR-12 AT 10:24 BST AT 10:24 BST by MGT Biochemical and biophysical research communications 2020 Only protein-protein interactions imex curation imex curation Interaction between IDH1 WT and calmodulin and its implications for glioblastoma cell growth and migration. Interaction between IDH1 WT and calmodulin and its implications for glioblastoma cell growth and migration. Biochemical and biophysical research communications 2020 imex curation Kang S., Kwon HN., Kang S., Park S. Cancer - Interactions investigated in the context of cancer Rare diseases - Interactions investigated in the context of Rare genetic disease lovesmart@snu.ac.kr,psh@snu.ac.kr 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Kang S., Kwon HN., Kang S., Park S. Rare diseases - Interactions investigated in the context of Rare genetic disease lovesmart@snu.ac.kr,psh@snu.ac.kr Accepted 2021-APR-12 AT 10:24 BST AT 10:24 BST by MGT Biochemical and biophysical research communications 2020 Only protein-protein interactions imex curation imex curation calm1_human Calmodulin-1 CALM1 CALM CAM CAM1 protein protein human Homo sapiens Human MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK 6B4BC3FCDE10727B idhc_human Isocitrate dehydrogenase [NADP] cytoplasmic Cytosolic NADP-isocitrate dehydrogenase Oxalosuccinate decarboxylase NADP(+)-specific ICDH IDP IDH1 PICD protein protein human Homo sapiens Human MSKKISGGSVVEMQGDEMTRIIWELIKEKLIFPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKHNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVSGWVKPIIIGRHAYGDQYRATDFVVPGPGKVEITYTPSDGTQKVTYLVHNFEEGGGVAMGMYNQDKSIEDFAHSSFQMALSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSQFEAQKIWYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLVCPDGKTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWTRGLAHRAKLDNNKELAFFANALEEVSIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKIKLAQAKL DIP protein O75874 original sequence version: 127 DIP protein O75874 original sequence version: 168 60428B0B6E5851DC trifluoperazine trifluoperazine 10-[3-(4-methyl-1-piperazinyl)propyl]-2-(trifluoromethyl)-10H-phenothiazine 10-[3-(4-METHYL-PIPERAZIN-1-YL)-PROPYL]-2-TRIFLUOROMETHYL-10H-PHENOTHIAZINE trifluoperazina Trifluoperazine trifluoperazine trifluoperazinum trifluoromethyl-10-(3'-(1-methyl-4-piperazinyl)propyl)phenothiazine trifluoroperazine trifluperazine 10-[3-(4-methylpiperazin-1-yl)propyl]-2-(trifluoromethyl)-10H-phenothiazine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) calm1-idh1-1 2 8 unspecified role unspecified role prey prey purified purified O75874:p.Arg132His mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R H ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction 4 1c, SF1 calm1-idh1-2 2 8 unspecified role unspecified role prey prey purified purified O75874:p.Arg132His mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R H ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction 3 SF1 calm1-idh1-3 6 7 unspecified role unspecified role bait bait purified purified ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey purified purified O75874:p.Arg132His mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R H ecoli Escherichia coli (strain K12) direct interaction direct interaction 1d calm1-idh1-4 1 7 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell O75874:p.Arg132His mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R H physical association physical association 1f calm1-idh1-5 2 8 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait purified purified ecoli Escherichia coli (strain K12) 8 competitor competitor competitor neutral component neutral component binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 2b calm1-idh1-6 5 7 unspecified role unspecified role neutral component neutral component region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 2c-d Calcium (CHEBI:29108) calm1-idh1-7 2 9 inhibitor inhibitor neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction 3a