IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The microRNAs miR-302b and miR-372 regulate mitochondrial metabolism via the SLC25A12 transporter, which controls MAVS-mediated antiviral innate immunity. The microRNAs miR-302b and miR-372 regulate mitochondrial metabolism via the SLC25A12 transporter, which controls MAVS-mediated antiviral innate immunity. The Journal of biological chemistry 2020 imex curation Yasukawa K., Kinoshita D., Yaku K., Nakagawa T., Koshiba T. koshiba@kyudai.jp 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag luciferase tag visualisation by luciferase assay tag luciferase Yasukawa K., Kinoshita D., Yaku K., Nakagawa T., Koshiba T. koshiba@kyudai.jp Accepted 2021-MAR-05 AT 14:10 GMT AT 14:10 GMT by KALPANAP The Journal of biological chemistry 2020 Only protein-protein interactions imex curation imex curation The microRNAs miR-302b and miR-372 regulate mitochondrial metabolism via the SLC25A12 transporter, which controls MAVS-mediated antiviral innate immunity. The microRNAs miR-302b and miR-372 regulate mitochondrial metabolism via the SLC25A12 transporter, which controls MAVS-mediated antiviral innate immunity. The Journal of biological chemistry 2020 imex curation Yasukawa K., Kinoshita D., Yaku K., Nakagawa T., Koshiba T. koshiba@kyudai.jp 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy anti-tag immunocytochemistry anti-tag immunocytochemistry Yasukawa K., Kinoshita D., Yaku K., Nakagawa T., Koshiba T. koshiba@kyudai.jp Accepted 2021-MAR-05 AT 14:10 GMT AT 14:10 GMT by KALPANAP The Journal of biological chemistry 2020 Only protein-protein interactions imex curation imex curation The microRNAs miR-302b and miR-372 regulate mitochondrial metabolism via the SLC25A12 transporter, which controls MAVS-mediated antiviral innate immunity. The microRNAs miR-302b and miR-372 regulate mitochondrial metabolism via the SLC25A12 transporter, which controls MAVS-mediated antiviral innate immunity. The Journal of biological chemistry 2020 imex curation Yasukawa K., Kinoshita D., Yaku K., Nakagawa T., Koshiba T. koshiba@kyudai.jp 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip anti tag western anti tag western blot anti tag western Yasukawa K., Kinoshita D., Yaku K., Nakagawa T., Koshiba T. koshiba@kyudai.jp Accepted 2021-MAR-05 AT 14:10 GMT AT 14:10 GMT by KALPANAP The Journal of biological chemistry 2020 Only protein-protein interactions imex curation imex curation The microRNAs miR-302b and miR-372 regulate mitochondrial metabolism via the SLC25A12 transporter, which controls MAVS-mediated antiviral innate immunity. The microRNAs miR-302b and miR-372 regulate mitochondrial metabolism via the SLC25A12 transporter, which controls MAVS-mediated antiviral innate immunity. The Journal of biological chemistry 2020 imex curation Yasukawa K., Kinoshita D., Yaku K., Nakagawa T., Koshiba T. koshiba@kyudai.jp 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip western blot western blot Immuno blot Immuno blot Yasukawa K., Kinoshita D., Yaku K., Nakagawa T., Koshiba T. koshiba@kyudai.jp Accepted 2021-MAR-05 AT 14:10 GMT AT 14:10 GMT by KALPANAP The Journal of biological chemistry 2020 Only protein-protein interactions imex curation imex curation mrna_mpc1 mitochondrial pyruvate carrier 1 mRNA mrna messenger rna mRNA mrna human Homo sapiens Human GTCATTGGCTCTGGGAAGCGGCAGCAGAGGCAGGGACCACTCGGGGTCTGGTGTCGGCACAGCCATGGCGGGCGCGTTGGTGCGGAAAGCGGCGGACTATGTCCGAAGCAAGGATTTCCGGGACTACCTCATGAGTACGCACTTCTGGGGCCCAGTAGCCAACTGGGGTCTTCCCATTGCTGCCATCAATGATATGAAAAAGTCTCCAGAGATTATCAGTGGGCGGATGACATTTGCCCTCTGTTGCTATTCTTTGACATTCATGAGATTTGCCTACAAGGTACAGCCTCGGAACTGGCTTCTGTTTGCATGCCACGCAACAAATGAAGTAGCCCAGCTCATCCAGGGAGGGCGGCTTATCAAACACGAGATGACTAAAACGGCATCTGCATAACAATGGAAAAGGAAGAACAAGGTCTTGAAGGGACAGCATTGCCAGCTGCTGCTGAGTCACAGATTTCATTATAAATAGCCTCCCTAAGGAAAATACACTGAATGCTATTTTTACTAACCATTCTATTTTTATAGAAATAGCTGAGAGTTTCTAAACCAACTCTCTGCTGCCTTACAAGTATTAAATATTTTACTTCTTTCCATAAAGAGTAGCTCAAAATATGCAATTAATTTAATAATTTCTGATGATGGTTTTATCTGCAGTAATATGTATATCATCTATTAGAATTTACTTAATGAAAAACTGAAGAGAACAAAATTTGTAACCACTAGCACTTAAGTACTCCTGATTCTTAACATTGTCTTTAATGACCACAAGACAACCAACAGCTGGCCACGTACTTAAAATTTTGTCCCCACTGTTTAAAAATGTTACCTGTGTATTTCCATGCAGTGTATATATTGAGATGCTGTAACTTAATGGCAATAAATGATTTAAATATTTGTTAAA cmc1_human Calcium-binding mitochondrial carrier protein Aralar1 SLC25A12 ARALAR1 Mitochondrial aspartate glutamate carrier 1 Solute carrier family 25 member 12 protein protein human Homo sapiens Human MAVKVQTTKRGDPHELRNIFLQYASTEVDGERYMTPEDFVQRYLGLYNDPNSNPKIVQLLAGVADQTKDGLISYQEFLAFESVLCAPDSMFIVAFQLFDKSGNGEVTFENVKEIFGQTIIHHHIPFNWDCEFIRLHFGHNRKKHLNYTEFTQFLQELQLEHARQAFALKDKSKSGMISGLDFSDIMVTIRSHMLTPFVEENLVSAAGGSISHQVSFSYFNAFNSLLNNMELVRKIYSTLAGTRKDVEVTKEEFAQSAIRYGQVTPLEIDILYQLADLYNASGRLTLADIERIAPLAEGALPYNLAELQRQQSPGLGRPIWLQIAESAYRFTLGSVAGAVGATAVYPIDLVKTRMQNQRGSGSVVGELMYKNSFDCFKKVLRYEGFFGLYRGLIPQLIGVAPEKAIKLTVNDFVRDKFTRRDGSVPLPAEVLAGGCAGGSQVIFTNPLEIVKIRLQVAGEITTGPRVSALNVLRDLGIFGLYKGAKACFLRDIPFSAIYFPVYAHCKLLLADENGHVGGLNLLAAGAMAGVPAASLVTPADVIKTRLQVAARAGQTTYSGVIDCFRKILREEGPSAFWKGTAARVFRSSPQFGVTLVTYELLQRWFYIDFGGLKPAGSEPTPKSRIADLPPANPDHIGGYRLATATFAGIENKFGLYLPKFKSPSVAVVQPKAAVAATQ D00A7D3D4244539F mavs_human Mitochondrial antiviral-signaling protein Interferon beta promoter stimulator protein 1 Virus-induced-signaling adapter CARD adapter inducing interferon beta Putative NF-kappa-B-activating protein 031N MAVS IPS1 KIAA1271 VISA protein protein human Homo sapiens Human MPFAEDKTYKYICRNFSNFCNVDVVEILPYLPCLTARDQDRLRATCTLSGNRDTLWHLFNTLQRRPGWVEYFIAALRGCELVDLADEVASVYQSYQPRTSDRPPDPLEPPSLPAERPGPPTPAAAHSIPYNSCREKEPSYPMPVQETQAPESPGENSEQALQTLSPRAIPRNPDGGPLESSSDLAALSPLTSSGHQEQDTELGSTHTAGATSSLTPSRGPVSPSVSFQPLARSTPRASRLPGPTGSVVSTGTSFSSSSPGLASAGAAEGKQGAESDQAEPIICSSGAEAPANSLPSKVPTTLMPVNTVALKVPANPASVSTVPSKLPTSSKPPGAVPSNALTNPAPSKLPINSTRAGMVPSKVPTSMVLTKVSASTVPTDGSSRNEETPAAPTPAGATGGSSAWLDSSSENRGLGSELSKPGVLASQVDSPFSGCFEDLAISASTSLGMGPCHGPEENEYKSEGTFGIHVAENPSIQLLEGNPGPPADPDGGPRPQADRKFQEREVPCHRPSPGALWLQVAVTGVLVVTLLVVLYRRRLH 0E23E3E115941EE8 phb_human Prohibitin PHB PHB1 protein protein human Homo sapiens Human MAAKVFESIGKFGLALAVAGGVVNSALYNVDAGHRAVIFDRFRGVQDIVVGEGTHFLIPWVQKPIIFDCRSRPRNVPVITGSKDLQNVNITLRILFRPVASQLPRIFTSIGEDYDERVLPSITTEILKSVVARFDAGELITQRELVSRQVSDDLTERAATFGLILDDVSLTHLTFGKEFTEAVEAKQVAQQEAERARFVVEKAEQQKKAAIISAEGDSKAAELIANSLATAGDGLIELRKLEAAEDIAYQLSRSRNITYLPAGQSVLLQLPQ 915494273E342C76 phb2_human Prohibitin-2 BAP REA PHB2 D-prohibitin B-cell receptor-associated protein BAP37 Repressor of estrogen receptor activity protein protein human Homo sapiens Human MAQNLKDLAGRLPAGPRGMGTALKLLLGAGAVAYGVRESVFTVEGGHRAIFFNRIGGVQQDTILAEGLHFRIPWFQYPIIYDIRARPRKISSPTGSKDLQMVNISLRVLSRPNAQELPSMYQRLGLDYEERVLPSIVNEVLKSVVAKFNASQLITQRAQVSLLIRRELTERAKDFSLILDDVAITELSFSREYTAAVEAKQVAQQEAQRAQFLVEKAKQEQRQKIVQAEGEAEAAKMLGEALSKNPGYIKLRKIRAAQNISKTIATSQNRIYLTADNLVLNLQDESFTRGSDSLIKGKK A887CC982BF85C80 hsa-mir-302b-3p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA human Homo sapiens Human UAAGUGCUUCCAUGUUUUAGUAG mrna_slc25a12 solute carrier family 25 member 12, mRNA mrna messenger rna mRNA mrna human Homo sapiens Human ACTCGCTCGTCCCCGGCTTCCGAGCACAGCATGGCGGTCAAGGTGCAGACAACTAAGCGAGGGGATCCTCATGAGTTAAGAAACATATTTCTACAGTATGCCAGTACTGAGGTTGATGGAGAGCGTTATATGACCCCAGAAGACTTTGTTCAGCGCTATCTTGGACTGTATAATGATCCAAATAGTAACCCAAAGATCGTGCAGCTCTTGGCAGGAGTAGCTGATCAAACCAAGGATGGGTTGATCTCCTATCAAGAGTTTTTGGCATTTGAATCTGTTTTATGTGCTCCAGATTCCATGTTCATAGTGGCTTTCCAGTTGTTTGACAAGAGTGGAAATGGAGAGGTGACATTTGAAAATGTCAAAGAAATTTTTGGACAGACTATTATTCATCATCATATCCCTTTTAACTGGGATTGTGAATTTATCCGACTGCATTTTGGGCATAACCGGAAGAAGCATCTTAACTACACAGAATTCACGCAGTTTCTCCAGGAGCTGCAATTGGAACATGCAAGACAAGCCTTTGCACTCAAAGACAAAAGCAAAAGTGGCATGATTTCTGGTCTGGATTTCAGTGACATCATGGTTACCATTAGATCTCACATGCTTACTCCTTTTGTGGAGGAGAACTTAGTTTCAGCAGCTGGAGGAAGTATCTCACACCAGGTTAGCTTCTCCTACTTCAATGCATTTAACTCGTTACTGAATAACATGGAGCTTGTTCGTAAGATATATAGCACTCTAGCTGGCACAAGGAAAGATGTTGAAGTCACAAAGGAGGAATTTGCCCAGAGTGCCATACGCTATGGACAAGTCACACCACTAGAAATTGATATTCTATATCAGCTTGCAGACTTATATAATGCTTCAGGGCGCTTGACTTTGGCAGATATTGAGAGAATAGCCCCATTGGCTGAGGGGGCCTTACCTTACAACCTGGCAGAACTTCAGAGACAGCAGTCTCCTGGGTTAGGCAGGCCTATCTGGCTCCAGATTGCCGAGTCTGCTTACAGATTCACTCTGGGCTCAGTTGCTGGAGCTGTGGGAGCCACTGCAGTGTATCCTATAGATCTGGTGAAGACCCGAATGCAAAACCAGCGTGGCTCTGGCTCTGTTGTTGGGGAGCTAATGTACAAAAACAGCTTTGACTGTTTTAAGAAAGTCTTGCGTTATGAGGGCTTCTTTGGACTCTACAGGGGTCTGATACCACAACTTATAGGGGTTGCTCCAGAAAAGGCCATTAAACTGACTGTTAATGATTTTGTTCGGGACAAATTTACCAGAAGAGATGGCTCTGTTCCACTTCCAGCAGAAGTTCTTGCTGGAGGCTGTGCTGGAGGCTCTCAGGTCATTTTTACCAACCCATTGGAGATAGTGAAGATTCGTCTGCAAGTAGCTGGAGAGATCACCACGGGACCCAGAGTCAGCGCCCTGAATGTGCTCCGGGACTTGGGAATTTTTGGTCTGTATAAGGGTGCCAAAGCGTGTTTCCTCCGAGACATTCCCTTCTCTGCAATCTATTTTCCTGTTTATGCTCATTGCAAACTACTTCTGGCTGATGAAAATGGACACGTGGGAGGTTTAAATCTTCTTGCAGCTGGAGCCATGGCAGGTGTCCCAGCTGCATCTCTGGTGACCCCTGCTGATGTCATCAAGACAAGACTGCAGGTGGCTGCCCGCGCTGGCCAGACGACATACAGTGGTGTCATCGACTGTTTCAGGAAGATTCTCCGGGAAGAAGGGCCCTCAGCATTTTGGAAAGGGACTGCAGCTCGAGTGTTTCGATCCTCTCCCCAGTTTGGTGTTACCTTGGTCACTTATGAACTTCTCCAGCGGTGGTTTTACATTGATTTTGGAGGCCTCAAACCCGCTGGTTCAGAACCAACACCTAAGTCACGCATTGCAGACCTTCCTCCTGCCAACCCTGATCACATCGGTGGATACAGACTCGCCACAGCCACGTTTGCAGGCATCGAAAACAAATTTGGCCTTTATCTCCCGAAATTTAAGTCTCCTAGTGTTGCTGTGGTTCAGCCAAAGGCAGCAGTGGCAGCCACTCAGTGATGAGACAACTGTTGAGTGTGGCAAAATGGCGCCTTGAAGAAAGAGGCCTAGGAGAGCAGCCCTGTAATGTATCCAGTCAGCTGCATGGTACTGACTGAGCTGAGGAGTCAAACTCTTCTTTCTGTATGACATATACATATACTTGTTTATAAAATAATCATTTGCCCAGGGAAAAAACCACAACGCTGTTTCAAGCTTTAGTCTTATGTGTTGAAATGTTTTTGTAAGCCTTGGCATGAATTAGTGTTCTAGACTCTGCTTTGCACAGCTTGCACTTACAGTGATTGTACATATTGTACATCTTTGTACAGAGACATCTTGGCACCTCATCCCAACAAATCACATTTGTAGAAATGTAATGCGGTTCTGAGTGGCTTGAAATGTACAGAATGTTTTGAAAGTGTTTTATTAAGAATCACACAAAAATAAATGTATTAAAATTAAATTCATTCTCTTATTGGTGACTTATGGAAATAAAGCATCAATATTGGATGTATTTAATTCCTAGTTTGTTTTCCATTCTGGAATAAAAAGGTATTTGCTGATAAAAGGCATAACGAGACATAGTGCTGCTACCACTGAATAAGTGATACTTTGGAAAGATGCATGCCAGTGGATGCCAGAGGACCAGGCTAATGACTTGTGTGTGCTGATGTGTTTCCATTTGTATTTAATGTGTGTAGACCCTCCTCTGTTCATCAATCAAAAAGCATTTCCTAGGCAGCTCCTCGCCTGTCAGTGTGCATATGGAAACAGGGACATCTCCATCATTACTGGCTTAGTTTTGCTTTCCTTTGACACAGTAAGGCAAAGGCCAAGCTTTCAAAAGAGTAAAGGATACTTTCACAATTTCCCTTCATATGGATATGATTCCAGTCAAAAATAAAATGCACACCAAAATGTATACATGTAGTTGGATTTTTCTTTGTAAATATACTGATAATACTTTTTGTTCTGATTGACTACTGATTGCAAATAACTCATGATCGGGTTCGCCCTTTCTTCCACCTTTATAGAAAACAGAAAAATTAGTTTTAGAAAGGGCTAGCGCAGAGTGAAGGGAGGGAAGAAGAAAGAGAAGAAAAGCTCTATATGGTGATTTAGTGGGGTCTAGGTTCTTACTTTGGAATCCAAATGCAAACTGTTCTAAAAGTCACTCATCAAGGGCGTGATTACTAATTTGGATCTAAACATAGATCTTGACAGCCACATCTACACTTTGATATGGATGTGATTTGATGTGCAGTATCAGAATTTATTTACCTTACCAAAGTGCTCTGGGAAGACATTATTGCTACTGCCAGTAAGCTGGGATATGAGAATGTATGCAAATATTTTCAAGAGGCGAGCCTGGAGACAGACCAAGTTACTTAGTATCCTGCACAGTGCTCCTCGATTGATGGCTGACAGCCCTGTGACTGCAACTGTAGGCTCCCTGGAGGCCCTCCAGGAACAAAAGAATTAGCTTTGAAAGCAAGTTATTTTCAAATGTATTAGATTGGTCTCATGGGAGTGGAACAAATAATAATACAGTTGTTTTGCTACTACCACTTGTTAGAAATATATAAGTAGTCCATGGATATGTGATTACAAAAGAATCCTATGTATGTATCTAGAGTGAGATGGGAAAGTGTCCCGCTTACCCTCCTTCTACCTCCACCACTTCCTGGCCCAGAGGTAATCACTGCTAACAGGTTACAGCATGAATATGTAGTCTGTTTTTCATAACTCTATAGAAATATATGTACATATTTATATTTTAGAAACAGGATTATGTTATGTGTGTGTTATTCTGTGTCTTGCTTTTGTTCACTTAATGTGTTTTGGATATCTTTCCATGTCAGTTTATAAAGTTTTATTTTTAATGGC mrna_rab32 RAB32, member RAS oncogene family mrna messenger rna mRNA mrna human Homo sapiens Human AGACTCGGAGTCGAGGCGCGCCCGACAGCCGCAGCGCTCATGGCGGGCGGAGGAGCCGGGGACCCCGGCCTGGGGGCGGCCGCCGCCCCAGCGCCCGAGACCCGCGAGCACCTCTTCAAGGTGCTGGTGATCGGCGAGCTTGGCGTGGGCAAGACCAGCATCATCAAGCGCTACGTCCACCAGCTCTTCTCCCAGCACTACCGGGCCACCATCGGGGTGGACTTCGCCCTCAAGGTCCTCAACTGGGACAGCAGGACTCTGGTGCGCCTGCAGCTGTGGGACATCGCGGGGCAGGAGCGATTTGGCAACATGACCCGAGTATACTACAAGGAAGCTGTTGGTGCTTTTGTAGTCTTTGATATATCAAGAAGTTCCACATTTGAGGCAGTCTTAAAATGGAAAAGTGATCTGGATAGTAAAGTTCATCTTCCAAATGGCAGCCCTATCCCTGCTGTCCTCTTGGCTAACAAATGTGACCAGAACAAGGACAGTAGCCAGAGTCCTTCCCAGGTGGACCAATTCTGCAAAGAACATGGCTTTGCCGGATGGTTTGAAACCTCTGCAAAGGATAACATAAACATAGAGGAAGCTGCCCGGTTCCTAGTGGAGAAGATTCTTGTAAACCACCAAAGCTTTCCTAATGAAGAAAACGATGTGGACAAAATTAAGCTAGATCAAGAGACCTTGAGAGCAGAGAACAAATCCCAGTGTTGCTGATATATGGCTTCTGCTTCTCTTGTGTGTGCCTCAGCTCTGAAGAAGTTCCTGAGAATGGGTTACAGATGTCATGTTAGCTGGGAGTCTTCCCACATGTGGCACTTCAAAAGGCAGCACCACTGGGCGCCTGCACTTATTTGAAAATGGAACTTTGGGAGAAGTATCCCTGCTAGTGGCTCTGTAACTTAACAGATGACAATTAGGCTTTTGTCATTGTTGCCATCATATGGAAGATAATGTTTACATCCTTTTAAACATTTTTATATGACAATTCCTCAGGATTTGGTAAGGCTTCCAAGTTGTAGCTTTTAGTGTAAGTGCTGGGGTGGTAATAAAATGTTACCTCCA hsa-mir-372-3p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA human Homo sapiens Human AAAGUGCUGCGACAUUUGAGCGU hsa_mir_302b_3p-mrna_rab32-1 1 12 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered tag luciferase tag luciferase tag 3' range 3' range 3' range 3' range ENST00000367495:r.847-851delinscgaaa mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain GCACT CGAAA 10 predetermined predetermined participant predetermined tag luciferase tag visualisation by luciferase assay tag luciferase regulator regulator modulator regulator neutral component neutral component engineered engineered purified purified physical association physical association down-regulates quantity by destabilization Fig 3 D hsa_mir_302b_3p-mrna_slc25a12-1 1 11 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered tag luciferase tag luciferase tag 3' range 3' range 3' range 3' range ENST00000422440:r.[2332_2334delinstgt; 2339_2343delinscgagat] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain ACA TGT certain certain sequence position certain certain certain sequence position certain GCACT CGAGA 10 predetermined predetermined participant predetermined tag luciferase tag visualisation by luciferase assay tag luciferase regulator regulator modulator regulator neutral component neutral component engineered engineered purified purified physical association physical association down-regulates quantity by destabilization Fig 4 B hsa_mir_372_3p-mrna_rab32-1 1 13 predetermined predetermined participant predetermined tag luciferase tag visualisation by luciferase assay tag luciferase regulator regulator modulator regulator neutral component neutral component engineered engineered purified purified 12 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered tag luciferase tag luciferase tag 3' range 3' range 3' range 3' range ENST00000367495:r.847-851delinscgaaa mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain GCACT CGAAA physical association physical association down-regulates quantity by destabilization Suppl Fig 2 C hsa_mir_372_3p-mrna_slc25a12-1 1 11 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered tag luciferase tag luciferase tag 3' range 3' range 3' range 3' range ENST00000422440:r.[2332_2334delinstgt; 2339_2343delinscgagat] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain ACA TGT certain certain sequence position certain certain certain sequence position certain GCACT CGAGA mutation range 2332-2334 probably does not affect this interaction 13 predetermined predetermined participant predetermined tag luciferase tag visualisation by luciferase assay tag luciferase regulator regulator modulator regulator neutral component neutral component engineered engineered purified purified physical association physical association down-regulates quantity by destabilization Suppl Fig 3 A slc25a12-mavs-1 4 7 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 9 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 6 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level association association Fig 5 A slc25a12-mavs-2 3 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 5 B slc25a12-mavs-3 2 7 unspecified role unspecified role neutral component neutral component living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig 5 B hsa_mir_302b_3p-mrna_mpc1-1 1 10 predetermined predetermined participant predetermined tag luciferase tag visualisation by luciferase assay tag luciferase regulator regulator modulator regulator neutral component neutral component engineered engineered purified purified 5 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered ENST00000360961:r.718_730delinsttgcactacgaga mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain AACCACTAGCACT TTGCACTACGAGA tag luciferase tag luciferase tag 3' range 3' range 3' range 3' range physical association physical association down-regulates quantity by destabilization Suppl Fig 4 D