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  <entry>
    <source releaseDate="2022-02-04">
      <names>
        <shortLabel>DIP</shortLabel>
        <fullName>Database of Interacting Proteins</fullName>
      </names>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0465" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="pubmed" dbAc="MI:0446" id="14681454" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1579232" refType="identity" refTypeAc="MI:0356"/>
      </xref>
      <attributeList>
        <attribute name="postaladdress">611 Young Drive East; Los Angeles CA 90095; USA</attribute>
        <attribute name="url" nameAc="MI:0614">http://dip.doe-mbi.ucla.edu/</attribute>
        <attribute name="contact-email" nameAc="MI:0634">dip@mbi.ucla.edu</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Cryo-EM structures of the DCPIB-inhibited volume-regulated anion channel LRRC8A in lipid nanodiscs.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="30775971" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-27096130" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="imex" dbAc="MI:0670" id="IM-29274" refType="imex-primary" refTypeAc="MI:0662"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Cryo-EM structures of the DCPIB-inhibited volume-regulated anion channel LRRC8A in lipid nanodiscs.</attribute>
            <attribute name="journal" nameAc="MI:0885">eLife</attribute>
            <attribute name="publication year" nameAc="MI:0886">2019</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Kern DM., Oh S., Hite RK., Brohawn SG.</attribute>
            <attribute name="contact-email" nameAc="MI:0634">brohawn@berkeley.edu</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959">imex curation</attribute>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="pubmed" dbAc="MI:0446" id="30775971" refType="primary-reference" refTypeAc="MI:0358"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-29274" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="7108">
            <names>
              <shortLabel>spofr-sf_9</shortLabel>
              <fullName>Spodoptera frugiperda insect cells</fullName>
              <alias type="synonym" typeAc="MI:1041">Fall armyworm</alias>
            </names>
            <cellType>
              <names>
                <shortLabel>sf_9</shortLabel>
                <fullName>insect cells</fullName>
              </names>
              <xref>
                <primaryRef db="cabri" dbAc="MI:0246" id="ACC 125" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="IA:0078" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="mint" dbAc="MI:0471" id="MINT-86941" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-346407" refType="identity" refTypeAc="MI:0356"/>
              </xref>
              <attributeList>
                <attribute name="comment" nameAc="MI:0612">Derived from pupal ovarian tissue of the fall armyworm</attribute>
                <attribute name="comment" nameAc="MI:0612">Cloned from the parent cell line, IPLB-SF-21-AE (SF-21)</attribute>
                <attribute name="comment" nameAc="MI:0612">derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae</attribute>
                <attribute name="comment" nameAc="MI:0612">cells are susceptible to Baculovirus infection</attribute>
              </attributeList>
            </cellType>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>3d-em</shortLabel>
            <fullName>3D electron microscopy</fullName>
            <alias type="synonym" typeAc="MI:1041">3D-EM</alias>
            <alias type="synonym" typeAc="MI:1041">electron tomog</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0410" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608522" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="12160704" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>experimental particp</shortLabel>
            <fullName>experimental participant identification</fullName>
            <alias type="synonym" typeAc="MI:1041">experimental particp</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0661" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-933978" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Kern DM., Oh S., Hite RK., Brohawn SG.</attribute>
          <attribute name="contact-email" nameAc="MI:0634">brohawn@berkeley.edu</attribute>
          <attribute name="accepted">Accepted 2021-OCT-07 AT 12:36 BST AT 12:36 BST by MGT</attribute>
          <attribute name="journal" nameAc="MI:0885">eLife</attribute>
          <attribute name="publication year" nameAc="MI:0886">2019</attribute>
          <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
          <attribute name="imex curation" nameAc="MI:0959">imex curation</attribute>
          <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>lrc8a_mouse</shortLabel>
          <fullName>Volume-regulated anion channel subunit LRRC8A</fullName>
          <alias type="gene name synonym" typeAc="MI:0302">Protein ebouriffe</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Leucine-rich repeat-containing protein 8A</alias>
          <alias type="gene name" typeAc="MI:0301">Lrrc8a</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Lrrc8</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="Q80WG5" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSMUSP00000092690" version="SP_148" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSMUSP00000109284" version="SP_148" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSMUSP00000139038" version="SP_148" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="A2AQZ0" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-20718010" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSMUSG00000099041" version="SP_148" refType="gene"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSMUST00000095078" version="SP_148" refType="transcript"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSMUST00000113654" version="SP_148" refType="transcript"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSMUST00000139454" version="SP_148" refType="transcript"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0001678" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0002329" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005225" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005886" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005887" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0006820" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0006884" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0006970" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0007283" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0009986" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0015734" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0015810" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0016021" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0032024" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0034214" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0034702" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0042802" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0045663" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0098656" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0140360" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0140361" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:1902476" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:1905103" version="SP_148"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR001611" version="SP_148"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR003591" version="SP_148"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSMUSG00000007476" version="SP_148" refType="gene"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR021040" version="SP_148"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR032675" version="SP_148"/>
          <secondaryRef db="mint" dbAc="MI:0471" id="Q80WG5" version="SP_148"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6FNW" version="SP_148"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6G8Z" version="SP_148"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6G9L" version="SP_148"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6G9O" version="SP_148"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6NZW" version="SP_148"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6NZZ" version="SP_148"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6O00" version="SP_148"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="7M17" version="SP_148"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="7M19" version="SP_148"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-MMU-5223345" version="SP_148"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_808393.1"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="10090">
          <names>
            <shortLabel>mouse</shortLabel>
            <fullName>Mus musculus</fullName>
            <alias type="synonym" typeAc="MI:1041">Mouse</alias>
          </names>
        </organism>
        <sequence>MIPVTELRYFADTQPAYRILKPWWDVFTDYISIVMLMIAVFGGTLQVTQDKMICLPCKWVTKDSCNDSFRGWAASSPEPTYPNSTVLPTPDTGPTGIKYDLDRHQYNYVDAVCYENRLHWFAKYFPYLVLLHTLIFLACSNFWFKFPRTSSKLEHFVSILLKCFDSPWTTRALSETVVEESDPKPAFSKMNGSMDKKSSTVSEDVEATVPMLQRTKSRIEQGIVDRSETGVLDKKEGEQAKALFEKVKKFRTHVEEGDIVYRLYMRQTIIKVIKFALIICYTVYYVHNIKFDVDCTVDIESLTGYRTYRCAHPLATLFKILASFYISLVIFYGLICMYTLWWMLRRSLKKYSFESIREESSYSDIPDVKNDFAFMLHLIDQYDPLYSKRFAVFLSEVSENKLRQLNLNNEWTLDKLRQRLTKNAQDKLELHLFMLSGIPDTVFDLVELEVLKLELIPDVTIPPSIAQLTGLKELWLYHTAAKIEAPALAFLRENLRALHIKFTDIKEIPLWIYSLKTLEELHLTGNLSAENNRYIVIDGLRELKRLKVLRLKSNLSKLPQVVTDVGVHLQKLSINNEGTKLIVLNSLKKMVNLTELELIRCDLERIPHSIFSLHNLQEIDLKDNNLKTIEEIISFQHLHRLTCLKLWYNHIAYIPIQIGNLTNLERLYLNRNKIEKIPTQLFYCRKLRYLDLSHNNLTFLPADIGLLQNLQNLAVTANRIEALPPELFQCRKLRALHLGNNVLQSLPSRVGELTNLTQIELRGNRLECLPVELGECPLLKRSGLVVEEDLFSTLPPEVKERLWRADKEQA</sequence>
        <attributeList>
          <attribute name="crc64">E3B125BC9977DBB0</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="3" imexId="IM-29274-1">
        <names>
          <shortLabel>lrrc8a-7</shortLabel>
        </names>
        <xref>
          <primaryRef db="emdb" dbAc="MI:0936" id="EMD-0564" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="wwpdb" dbAc="MI:0805" id="6NZW" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="emdb" dbAc="MI:0936" id="EMD-0562" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="wwpdb" dbAc="MI:0805" id="6NZZ" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="emdb" dbAc="MI:0936" id="EMD-0563" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="wwpdb" dbAc="MI:0805" id="6O00" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-27097364" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-29274-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="4">
            <interactorRef>2</interactorRef>
            <participantIdentificationMethodList>
              <participantIdentificationMethod>
                <names>
                  <shortLabel>predetermined</shortLabel>
                  <fullName>predetermined participant</fullName>
                  <alias type="synonym" typeAc="MI:1041">predetermined</alias>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </participantIdentificationMethod>
              <participantIdentificationMethod>
                <names>
                  <shortLabel>structure determination</shortLabel>
                  <fullName>identification by structure determination</fullName>
                  <alias type="synonym" typeAc="MI:1041">identification by structure determination</alias>
                  <alias type="synonym" typeAc="MI:1041">structure determination</alias>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:2287" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-20597534" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="7877166" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </participantIdentificationMethod>
              <participantIdentificationMethod>
                <names>
                  <shortLabel>experimental particp</shortLabel>
                  <fullName>experimental participant identification</fullName>
                  <alias type="synonym" typeAc="MI:1041">experimental particp</alias>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0661" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-933978" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </participantIdentificationMethod>
            </participantIdentificationMethodList>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <experimentalPreparationList>
              <experimentalPreparation>
                <names>
                  <shortLabel>purified</shortLabel>
                  <fullName>purified</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0350" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537811" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalPreparation>
            </experimentalPreparationList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="7108">
                <names>
                  <shortLabel>spofr-sf_9</shortLabel>
                  <fullName>Spodoptera frugiperda insect cells</fullName>
                  <alias type="synonym" typeAc="MI:1041">Fall armyworm</alias>
                </names>
                <cellType>
                  <names>
                    <shortLabel>sf_9</shortLabel>
                    <fullName>insect cells</fullName>
                  </names>
                  <xref>
                    <primaryRef db="cabri" dbAc="MI:0246" id="ACC 125" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="IA:0078" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="mint" dbAc="MI:0471" id="MINT-86941" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-346407" refType="identity" refTypeAc="MI:0356"/>
                  </xref>
                  <attributeList>
                    <attribute name="comment" nameAc="MI:0612">Derived from pupal ovarian tissue of the fall armyworm</attribute>
                    <attribute name="comment" nameAc="MI:0612">Cloned from the parent cell line, IPLB-SF-21-AE (SF-21)</attribute>
                    <attribute name="comment" nameAc="MI:0612">derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae</attribute>
                    <attribute name="comment" nameAc="MI:0612">cells are susceptible to Baculovirus infection</attribute>
                  </attributeList>
                </cellType>
              </hostOrganism>
            </hostOrganismList>
            <stoichiometry value="6"/>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>physical association</shortLabel>
            <fullName>physical association</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">Table 1</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>