IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ miR-124 attenuates Japanese encephalitis virus replication by targeting DNM2. miR-124 attenuates Japanese encephalitis virus replication by targeting DNM2. Virology journal 2016 imex curation Yang S., Pei Y., Li X., Zhao S., Zhu M., Zhao A. zhumengjin@mail.hzau.edu.cn Only protein-protein interactions imex curation mesau-bhk21 Mesocricetus auratus_BHK21 bhk-21 Cells from kidneys of five unsexed, one-day-old hamsters BHK-21/C13 cell mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag luciferase tag visualisation by luciferase assay tag luciferase Yang S., Pei Y., Li X., Zhao S., Zhu M., Zhao A. zhumengjin@mail.hzau.edu.cn Accepted 2021-JUN-21 AT 12:42 BST AT 12:42 BST by MGT Virology journal 2016 Only protein-protein interactions imex curation imex curation mrna_dnm2_pig dynamin 2 mRNA mrna messenger rna mRNA mrna pig Sus scrofa (Pig) Pig GGGCGCAGCCCCAGGGCCCCGGGCGGGAGGTCGCTTGGGTCGGGTGTCGCCTGAGAACCGGATGAGGCGGCGACCGTGAGGCCGAGCCGGGAGCGGGCGTCGCGCCGAGGCCCGGGCGGACGGGGAGCAACGGCCGTGGACGCCGCGGGGCCGCGTCGTTGAAGGCAGGGGCGGGTGGGGAGCGCGGGGCGCTCGGGCCGGGGCCACCGGCGCCATGGGCAACCGCGGGATGGAAGAACTGATCCCGCTGGTCAACAAGCTGCAGGATGCCTTCAGCTCCATCGGCCAGAGCTGCCACCTGGACCTGCCGCAGATTGCCGTGGTGGGCGGCCAGAGCGCCGGCAAGAGCTCAGTGCTCGAGAACTTCGTGGGCCGGGACTTCCTTCCCCGAGGTTCAGGAATCGTCACTCGGCGGCCTCTCATTCTGCAGCTCATCTTCTCAAAAACAGAATATGCTGAGTTTTTACACTGCAAGTCCAAAAAGTTTACAGACTTTGAAGAAGTCCGACAGGAGATCGAAGCGGAGACCGACAGGGTCACGGGGACCAATAAAGGCATCTCCCCAGTGCCCATCAACTTGCGGGTCTACTCACCACACGTGTTGAACCTGACCCTCATCGACCTGCCGGGCATCACCAAGGTGCCCGTGGGGGACCAGCCCCCAGACATCGAGTACCAGATCAAGGACATGATCCTGCAGTTCATCAGCCGGGAGAGCAGCCTCATCCTCGCCGTCACCCCCGCCAACATGGACCTGGCCAACTCGGACGCCCTCAAGCTGGCCAAGGAGGTCGACCCTCAAGGCCTGCGGACCATTGGCGTCATCACCAAGCTTGACCTGATGGACGAAGGCACTGATGCCAGGGATGTCCTGGAGAACAAGCTGCTCCCCTTGAGAAGAGGCTACATTGGTGTGGTGAACCGCAGCCAGAAGGACATCGAGGGCAAGAAGGACATTCGTGCAGCTCTGGCGGCTGAGAGGAAATTCTTCCTCTCCCACCCCGCCTACCGGCACATGGCTGACCGCATGGGCACCCCACACCTGCAGAAGACTCTGAATCAGCAACTGACCAATCACATCCGGGAGTCGCTGCCAGCCTTGCGCAGCAAGCTGCAGAGCCAGCTGCTGTCCTTGGAGAAGGAGGTGGAGGAGTATAAGAACTTCCGACCTGACGATCCCACTCGCAAGACCAAAGCTCTGCTGCAGATGGTCCAGCAATTTGGGGTGGACTTTGAGAAGAGGATCGAGGGTTCTGGAGACCAAGTGGACACGCTAGAGCTCTCCGGGGGCGCCCGAATCAATCGCATCTTCCATGAGCGGTTTCCCTTTGAGCTGGTGAAGATGGAGTTTGATGAGAAGGACTTAAGACGGGAGATCAGCTATGCCATTAAGAACATCCATGGAGTCAGGACCGGGCTCTTCACCCCGGACTTGGCATTCGAGGCCATTGTGAAAAAGCAGGTCGTCAAGCTGAAAGAGCCCTGTCTGAAATGTGTCGACCTGGTTATCCAGGAGCTAATCAATACAGTTAGGCAGTGTACCAGTAAGCTCAGCTCCTACCCCCGGCTGCGAGAGGAGACGGAACGAATCGTCACCACTTACATCCGGGAACGGGAGGGGAGAACCAAGGACCAGATTCTTCTTCTGATCGACATCGAGCAGTCCTACATCAACACGAACCACGAGGACTTCATTGGATTTGCCAAGTATGTACTTTTCAGGATAGTGGCTGGTGGGGCGGTGATCCGCCGGGGCTGGCTGACCATCAACAACATCAGCCTGATGAAGGGCGGCTCCAAGGAATACTGGTTTGTGCTGACAGCCGAGTCACTGTCCTGGTACAAGGATGAGGAGGAGAAAGAGAAGAAGTACATGCTGCCTCTGGACAACCTCAAAATCCGTGATGTGGAAAAGGGCTTCATGTCCAACAAGCATGTCTTCGCCATCTTCAACACAGAGCAGAGGAACGTCTACAAGGACCTGCGGCAGATCGAGCTGGCCTGTGACTCCCAGGAGGACGTGGACAGCTGGAAGGCCTCATTCCTCCGAGCCGGGGTCTACCCTGAGAAGGACCAGGTGAGGACCCAGGAGAACACCTTCTCCATGGACCCGCAGCTAGAGCGCCAGGTGGAGACCATTCGAAACCTGGTGGACTCATACGTGGCCATCATCAACAAGTCCATCCGTGATCTCATGCCAAAGACCATCATGCACCTCATGATCAATAACACGAAGGCCTTCATCCACCATGAGCTGCTGGCCTACCTGTACTCGTCAGCCGACCAGAGCAGCCTCATGGAGGAGTCAGCTGACCAGGCCCAGCGGCGGGACGACATGCTGCGCATGTACCATGCGCTCAAGGAGGCACTCAACATCATTGGGGACATCAGCACCAGCACCGTGTCCACGCCGGTGCCCCCGCCCGTTGATGACACCTGGCTCCAGAGCACCAGCAGCCACAGGTGTGCAAGGCCTGGTCACCCACAGGCCAGCTCCCCCCAAGTGTCCGTCAGGGCACAGCCCACGGGTGGGCGGGGTAAAGGGGCCCGACCACAGATGGTGCAGCACCCAGCTTGCCACAGTAGGGGCTGCCACCCCGCCTAGGCCTGAAGGCAGGTTAAGAGCTCGCCTGTCTCCTGGTTCCTTCCCTTGGCTAAGCTTAAACAGAAGCCAGAGAGAGCAGGGGGCCTGGGCAACCCTGGCCGTAGGGCTGAACCTACTGGAGCACAGAGCACAGCTGAGAATGGCAGGTGGGGAGAATCTGGATGGACACGTGGAGGATGTAACCAGCATCTCCCACCCTGACCACTTTAGAGCCCCACTCCGCAACGCCGGCCTGTGTCCAGCGTGCACCCCCCAGGCCGGCCCCCAGCAGTGAGGGGCCCCACCCCGGGGCCCCCCCTGATTCCTGTGCCGGTGGGAGCAGCAGCCTCCTTCTCCGCGCCCCCCATCCCATCCCGGCCTGGACCCCATCCTGGCGTGTTTGCCAACAATGACCCCTTCTCGGCCCCGCCTCAGATCCCATCTCGACCAGCTCGGATTCCACCCGGCATACCCCCCGGAGTCCCCAGCAGAAGACCCCCTGCTGCGCCCAGCCGGCCCACCATTATCCGCCCAGCCGAGCCATCCCTGCTCGACTAGGCTGCGGGGGGCACCTTCTCTGGGGTCCTCGAGCACCCTCGGTGCAGGAGCTTCATTGGTCTGGGCCCATCTGCCGCCCTTAGTCCTCCAGTCCCCATGCGGGACCAGGCTCTCTGTGGGCAGCCCCTGACTCTTCCCTAACCCCGGCCCCCAGGCAGGGGCTGGCCTGGCCCCCATCTCCAGCTGGACACAGCACTGAATGGAGGGGCCCTGGCAGCCCAGGATGAGGGCAGCCGGGGTGTTGCACTTTGGGGGATGGGGTCTCAGGTAGCAGAGCAGGCAGCTGCCGCCTGAGCACCATCTTGAATGAGGGAGCCAGGCTGAGGTGCCTCACCCAGGGGCTTCTCTGGCCAGGAAAGCCTGAGCAGACCAGGCCTTCTAGAAACAGCACCAAGGGAGTCTGCACCCCTAGTCCTTGCTGGGGCACAAGGAGTGTCTGCTTCTTGGCAGTGAGACCTCCCAGTGGAGAGCCAGGCCCAGTGGGCCCAGCGGGCCCAGCGCTACCCGCCCACCCAAGTTGTACATATTCCTTCCTTGGCCGTATTAACCGCACGGCCCAGCCTCGGCTGCCAGAGGTGCCTTTGCCAGGTCCGGAAAACCCTCGGCCCCGGCCAACCCCGTTCTCTCTTACCCGGGGCCTCCAGGGCAGCCTGGGCTGAGGGCTAAGGGGTGGAGAAGCCTCCTCCACCGGGTGAGCCCCGGGCAGCCTGTCTGAGCAGACCTTCTCGCTCGTTCGCTCGCTCGCTCGCTCGGTTTGACCACTGTAAGTGCCTGCACTCTGTATCCTATTAATAAACTAAAATAAAGGGAAGACGCTT hsa-mir124-3p Homo sapiens microRNA hsa-miR-124-3p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA human Homo sapiens Human UAAGGCACGCGGUGAAUGCC hsa_mir124_3p-mrna_dnm2_pig-1 1 3 predetermined predetermined participant predetermined tag luciferase tag visualisation by luciferase assay tag luciferase regulator regulator modulator regulator neutral component neutral component engineered engineered purified purified 2 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered tag luciferase tag luciferase tag 3' range 3' range 3' range 3' range ENSSSCT00000047677:r.3718-3724delinscacggaa mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain GTGCCTT CACGGAA ENSSSCT00000047677:r.3910-3915delinsaggcac mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain GTGCCT AGGCAC physical association physical association Fig 3 A B