UniProt UniProt http://www.uniprot.org Glyoxalate reductase/hydroxypyruvate reductase interacts with the sodium-dependent vitamin C transporter-1 to regulate cellular vitamin C homeostasis. Glyoxalate reductase/hydroxypyruvate reductase interacts with the sodium-dependent vitamin C transporter-1 to regulate cellular vitamin C homeostasis. American journal of physiology. Gastrointestinal and liver physiology 2013 imex curation Subramanian VS., Nabokina SM., Patton JR., Marchant JS., Moradi H., Said HM. vsubrama@uci.edu 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Subramanian VS., Nabokina SM., Patton JR., Marchant JS., Moradi H., Said HM. vsubrama@uci.edu Accepted 2021-MAR-10 AT 10:10 GMT AT 10:10 GMT by MGT Human liver cDNA American journal of physiology. Gastrointestinal and liver physiology 2013 Only protein-protein interactions imex curation imex curation Glyoxalate reductase/hydroxypyruvate reductase interacts with the sodium-dependent vitamin C transporter-1 to regulate cellular vitamin C homeostasis. Glyoxalate reductase/hydroxypyruvate reductase interacts with the sodium-dependent vitamin C transporter-1 to regulate cellular vitamin C homeostasis. American journal of physiology. Gastrointestinal and liver physiology 2013 imex curation Subramanian VS., Nabokina SM., Patton JR., Marchant JS., Moradi H., Said HM. vsubrama@uci.edu 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Subramanian VS., Nabokina SM., Patton JR., Marchant JS., Moradi H., Said HM. vsubrama@uci.edu Accepted 2021-MAR-10 AT 10:10 GMT AT 10:10 GMT by MGT Human liver cDNA American journal of physiology. Gastrointestinal and liver physiology 2013 Only protein-protein interactions imex curation imex curation Glyoxalate reductase/hydroxypyruvate reductase interacts with the sodium-dependent vitamin C transporter-1 to regulate cellular vitamin C homeostasis. Glyoxalate reductase/hydroxypyruvate reductase interacts with the sodium-dependent vitamin C transporter-1 to regulate cellular vitamin C homeostasis. American journal of physiology. Gastrointestinal and liver physiology 2013 imex curation Subramanian VS., Nabokina SM., Patton JR., Marchant JS., Moradi H., Said HM. vsubrama@uci.edu 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Subramanian VS., Nabokina SM., Patton JR., Marchant JS., Moradi H., Said HM. vsubrama@uci.edu Accepted 2021-MAR-10 AT 10:10 GMT AT 10:10 GMT by MGT Human liver cDNA American journal of physiology. Gastrointestinal and liver physiology 2013 Only protein-protein interactions imex curation imex curation Glyoxalate reductase/hydroxypyruvate reductase interacts with the sodium-dependent vitamin C transporter-1 to regulate cellular vitamin C homeostasis. Glyoxalate reductase/hydroxypyruvate reductase interacts with the sodium-dependent vitamin C transporter-1 to regulate cellular vitamin C homeostasis. American journal of physiology. Gastrointestinal and liver physiology 2013 imex curation Subramanian VS., Nabokina SM., Patton JR., Marchant JS., Moradi H., Said HM. vsubrama@uci.edu 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). gal4 vp16 complement gal4 vp16 complementation karyoplasmic interaction ion strategy KISS mammalian two hybrid gal4 vp16 complement predetermined predetermined participant predetermined Subramanian VS., Nabokina SM., Patton JR., Marchant JS., Moradi H., Said HM. vsubrama@uci.edu Accepted 2021-MAR-10 AT 10:10 GMT AT 10:10 GMT by MGT Human liver cDNA American journal of physiology. Gastrointestinal and liver physiology 2013 Only protein-protein interactions imex curation imex curation Glyoxalate reductase/hydroxypyruvate reductase interacts with the sodium-dependent vitamin C transporter-1 to regulate cellular vitamin C homeostasis. Glyoxalate reductase/hydroxypyruvate reductase interacts with the sodium-dependent vitamin C transporter-1 to regulate cellular vitamin C homeostasis. American journal of physiology. Gastrointestinal and liver physiology 2013 imex curation Subramanian VS., Nabokina SM., Patton JR., Marchant JS., Moradi H., Said HM. vsubrama@uci.edu 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Subramanian VS., Nabokina SM., Patton JR., Marchant JS., Moradi H., Said HM. vsubrama@uci.edu Accepted 2021-MAR-10 AT 10:10 GMT AT 10:10 GMT by MGT Human liver cDNA American journal of physiology. Gastrointestinal and liver physiology 2013 Only protein-protein interactions imex curation imex curation Glyoxalate reductase/hydroxypyruvate reductase interacts with the sodium-dependent vitamin C transporter-1 to regulate cellular vitamin C homeostasis. Glyoxalate reductase/hydroxypyruvate reductase interacts with the sodium-dependent vitamin C transporter-1 to regulate cellular vitamin C homeostasis. American journal of physiology. Gastrointestinal and liver physiology 2013 imex curation Subramanian VS., Nabokina SM., Patton JR., Marchant JS., Moradi H., Said HM. vsubrama@uci.edu 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Subramanian VS., Nabokina SM., Patton JR., Marchant JS., Moradi H., Said HM. vsubrama@uci.edu Accepted 2021-MAR-10 AT 10:10 GMT AT 10:10 GMT by MGT Human liver cDNA American journal of physiology. Gastrointestinal and liver physiology 2013 Only protein-protein interactions imex curation imex curation grhpr_human Glyoxylate reductase/hydroxypyruvate reductase MSTP035 GRHPR GLXR protein protein human Homo sapiens Human MRPVRLMKVFVTRRIPAEGRVALARAADCEVEQWDSDEPIPAKELERGVAGAHGLLCLLSDHVDKRILDAAGANLKVISTMSVGIDHLALDEIKKRGIRVGYTPDVLTDTTAELAVSLLLTTCRRLPEAIEEVKNGGWTSWKPLWLCGYGLTQSTVGIIGLGRIGQAIARRLKPFGVQRFLYTGRQPRPEEAAEFQAEFVSTPELAAQSDFIVVACSLTPATEGLCNKDFFQKMKETAVFINISRGDVVNQDDLYQALASGKIAAAGLDVTSPEPLPTNHPLLTLKNCVILPHIGSATHRTRNTMSLLAANNLLAGLRGEPMPSELKL 68A0E311AA4E5650 s23a1_human Solute carrier family 23 member 1 SLC23A1 SVCT1 YSPL3 Sodium-dependent vitamin C transporter 1 Na(+)/L-ascorbic acid transporter 1 Yolk sac permease-like molecule 3 protein protein human Homo sapiens Human MRAQEDLEGRTQHETTRDPSTPLPTEPKFDMLYKIEDVPPWYLCILLGFQHYLTCFSGTIAVPFLLAEALCVGHDQHMVSQLIGTIFTCVGITTLIQTTVGIRLPLFQASAFAFLVPAKAILALERWKCPPEEEIYGNWSLPLNTSHIWHPRIREVQGAIMVSSVVEVVIGLLGLPGALLNYIGPLTVTPTVSLIGLSVFQAAGDRAGSHWGISACSILLIILFSQYLRNLTFLLPVYRWGKGLTLLRIQIFKMFPIMLAIMTVWLLCYVLTLTDVLPTDPKAYGFQARTDARGDIMAIAPWIRIPYPCQWGLPTVTAAAVLGMFSATLAGIIESIGDYYACARLAGAPPPPVHAINRGIFTEGICCIIAGLLGTGNGSTSSSPNIGVLGITKVGSRRVVQYGAAIMLVLGTIGKFTALFSSLPDPILGGMFCTLFGMITAVGLSNLQFVDMNSSRNLFVLGFSMFFGLTLPNYLESNPGAINTGILEVDQILIVLLTTEMFVGGCLAFILDNTVPGSPEERGLIQWKAGAHANSDMSSSLKSYDFPIGMGIVKRITFLKYIPICPVFKGFSSSSKDQIAIPEDTPENTETASVCTKV 1191B2B43CE76FD6 grhpr-slc23a1-1 5 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association slc23a1-grhpr-1 1 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 1B grhpr-slc23a1-2 2 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). 7 unspecified role unspecified role bait bait purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range in vitro In vitro physical association physical association Fig. 2A grhpr-slc23a1-3 3 8 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 2B grhpr-slc23a1-4 6 8 unspecified role unspecified role neutral component neutral component cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 7 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig. 3C slc23a1-grhpr-2 4 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association Fig. 2D