MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it MeCP2 binds to 5hmC enriched within active genes and accessible chromatin in the nervous system. MeCP2 binds to 5hmC enriched within active genes and accessible chromatin in the nervous system. Cell 2012 imex curation Mellén M., Ayata P., Dewell S., Kriaucionis S., Heintz N. Brain Disease - Neurological Diseases & Disorders Rare diseases - Interactions investigated in the context of Rare genetic disease heintz@rockefeller.edu skirmantas.kriaucionis@ludwig.ox.ac.uk 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa autoradiography autoradiography Mellén M., Ayata P., Dewell S., Kriaucionis S., Heintz N. Brain Disease - Neurological Diseases & Disorders interactions with MBD-family proteins were not captured as origin species was not provided (email sent to authors on 9/2/2021) heintz@rockefeller.edu skirmantas.kriaucionis@ludwig.ox.ac.uk Accepted 2021-MAR-23 AT 10:14 GMT AT 10:14 GMT by KALPANAP Cell 2012 Only protein-protein interactions imex curation imex curation MeCP2 binds to 5hmC enriched within active genes and accessible chromatin in the nervous system. MeCP2 binds to 5hmC enriched within active genes and accessible chromatin in the nervous system. Cell 2012 imex curation Mellén M., Ayata P., Dewell S., Kriaucionis S., Heintz N. Brain Disease - Neurological Diseases & Disorders Rare diseases - Interactions investigated in the context of Rare genetic disease heintz@rockefeller.edu skirmantas.kriaucionis@ludwig.ox.ac.uk 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Mellén M., Ayata P., Dewell S., Kriaucionis S., Heintz N. Brain Disease - Neurological Diseases & Disorders interactions with MBD-family proteins were not captured as origin species was not provided (email sent to authors on 9/2/2021) heintz@rockefeller.edu skirmantas.kriaucionis@ludwig.ox.ac.uk Accepted 2021-MAR-23 AT 10:14 GMT AT 10:14 GMT by KALPANAP Cell 2012 Only protein-protein interactions imex curation imex curation 5-methylcytosine 5-methylcytosine 4-amino-5-methyl-2(1H)-pyrimidinone 4-Amino-5-methyl-2-pyrimidinol 5-Methylcytosine 5-methylcytosine 4-amino-5-methylpyrimidin-2(1H)-one small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) 5-(hydroxymethyl)cytosine 5-(hydroxymethyl)cytosine 4-Amino-5-(hydroxymethyl)-2(1H)-pyrimidinone 5-hydroxymethylcytosine 4-amino-5-(hydroxymethyl)pyrimidin-2(1H)-one small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) mecp2_human Methyl-CpG-binding protein 2 MECP2 protein protein human Homo sapiens Human MVAGMLGLREEKSEDQDLQGLKDKPLKFKKVKKDKKEEKEGKHEPVQPSAHHSAEPAEAGKAETSEGSGSAPAVPEASASPKQRRSIIRDRGPMYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVELIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSGTTRPKAATSEGVQVKRVLEKSPGKLLVKMPFQTSPGGKAEGGGATTSTQVMVIKRPGRKRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRETVSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHSESPKAPVPLLPPLPPPPPEPESSEDPTSPPEPQDLSSSVCKEEKMPRGGSLESDGCPKEPAKTQPAVATAATAAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERVS DIP protein P51608 original sequence version: 182 DIP protein P51608-1 original sequence version: 195 EB6A33233AEDA566 mecp2 -5mc-1 1 5 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P51608:p.Leu100Val mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L V P51608:p.Asp121Gly mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain D G P51608:p.Arg133His mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R H P51608:p.Arg133Cys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R C P51608:p.Ser134Cys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S C P51608:p.Ser134Phe mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S F P51608:p.Glu137Gly mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E G P51608:p.Ala140Val mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A V ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role bait bait purified purified 32p radiolabel 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Fig. 4C-D, Fig. 5A 5_methylcytosine-mecp2-1 1 5 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P51608:p.Leu100Val mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L V P51608:p.Asp121Gly mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain D G P51608:p.Arg133His mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R H P51608:p.Arg133Cys mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R C P51608:p.Ser134Cys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S C P51608:p.Ser134Phe mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S F P51608:p.Glu137Gly mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E G P51608:p.Ala140Val mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A V ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait purified purified 32p radiolabel 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Fig. 4C-D, Fig. 5A 5_(hydroxymethyl)cytosine-mecp2-1 2 4 unspecified role unspecified role bait bait purified purified biotin tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 5 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P51608:p.Arg133Cys mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R C ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 5B-C 5_(hydroxymethyl)cytosine-mecp2-2 2 3 unspecified role unspecified role bait bait purified purified biotin tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 5 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P51608:p.Arg133Cys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R C ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 5B-C