UniProt UniProt http://www.uniprot.org The endonuclease Ankle1 requires its LEM and GIY-YIG motifs for DNA cleavage in vivo. The endonuclease Ankle1 requires its LEM and GIY-YIG motifs for DNA cleavage in vivo. Journal of cell science 2012 imex curation Brachner A., Braun J., Ghodgaonkar M., Castor D., Zlopasa L., Ehrlich V., Jiricny J., Gotzmann J., Knasmüller S., Foisner R. roland.foisner@meduniwien.ac.at Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Brachner A., Braun J., Ghodgaonkar M., Castor D., Zlopasa L., Ehrlich V., Jiricny J., Gotzmann J., Knasmüller S., Foisner R. This experiment relates to a negative interaction. roland.foisner@meduniwien.ac.at Accepted 2021-AUG-09 AT 09:05 BST AT 09:05 BST by MGT Journal of cell science 2012 Only protein-protein interactions imex curation imex curation q8nag6-1 Ankyrin repeat and LEM domain-containing protein 1 Ankyrin repeat domain-containing protein 41 LEM-domain containing protein 3 ANKLE1 ANKRD41 LEM3 protein protein human Homo sapiens Human MRCGRRSRVEELLRCGADPNLVLEDGAAAVHLAAGARHPRGLRCLGALLRQGGDPNARSVEALTPLHVAAAWGCRRGLELLLSQGADPALRDQDGLRPLDLALQQGHLECARVLQDLDTRTRTRTRIGAETQEPEPAPGTPGLSGPTDETLDSIALQKQPCRGDNRDIGLEADPGPPSLPVPLETVDKHGSSASPPGHWDYSSDASFVTAVEVSGAEDPASDTPPWAGSLPPTRQGLLHVVHANQRVPRSQGTEAELNARLQALTLTPPNAAGFQSSPSSMPLLDRSPAHSPPRTPTPGASDCHCLWEHQTSIDSDMATLWLTEDEASSTGGREPVGPCRHLPVSTVSDLELLKGLRALGPEFSGHSLELAAALRTGCIPDVQADEDALAQQFEQPDPARRWREGVVKSSFTYLLLDPRETQDLPARAFSLTPAERLQTFIRAIFYVGKGTRARPYVHLWEALGHHGRSRKQPHQACPKVRQILDIWASGCGVVSLHCFQHVVAVEAYTREACIVEALGIQTLTNQKQGHCYGVVAGWPPARRRRLGVHLLHRALLVFLAEGERQLHPQDIQARG DDBB792FD87FA8EA baf_human Barrier-to-autointegration factor BANF1 BAF BCRG1 Breakpoint cluster region protein 1 protein protein human Homo sapiens Human MTTSQKHRDFVAEPMGEKPVGSLAGIGEVLGKKLEERGFDKAYVVLGQFLVLKKDEDLFREWLKDTCGANAKQSRDCFGCLREWCDAFL DIP protein O75531 original sequence version: 123 9A2180A2D284F5D0 ankle1-banf1-2 1 3 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate ecoli Escherichia coli (strain K12) 2 unspecified role unspecified role bait bait purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association true Fig. 3B