UniProt UniProt http://www.uniprot.org TEFM (c17orf42) is necessary for transcription of human mtDNA. TEFM (c17orf42) is necessary for transcription of human mtDNA. Nucleic acids research 2011 imex curation Minczuk M., He J., Duch AM., Ettema TJ., Chlebowski A., Dzionek K., Nijtmans LG., Huynen MA., Holt IJ. michal.minczuk@mrc-mbu.cam.ac.uk Only protein-protein interactions imex curation human-293 flp-in Homo sapiens 293 flp-in 293 flp-in pull down pull down anti tag western anti tag western blot anti tag western Minczuk M., He J., Duch AM., Ettema TJ., Chlebowski A., Dzionek K., Nijtmans LG., Huynen MA., Holt IJ. michal.minczuk@mrc-mbu.cam.ac.uk Accepted 2021-NOV-26 AT 16:10 GMT AT 16:10 GMT by MGT Nucleic acids research 2011 Only protein-protein interactions imex curation imex curation TEFM (c17orf42) is necessary for transcription of human mtDNA. TEFM (c17orf42) is necessary for transcription of human mtDNA. Nucleic acids research 2011 imex curation Minczuk M., He J., Duch AM., Ettema TJ., Chlebowski A., Dzionek K., Nijtmans LG., Huynen MA., Holt IJ. michal.minczuk@mrc-mbu.cam.ac.uk Only protein-protein interactions imex curation human-293 flp-in Homo sapiens 293 flp-in 293 flp-in pull down pull down sequence tag sequence tag identification sequence tag Minczuk M., He J., Duch AM., Ettema TJ., Chlebowski A., Dzionek K., Nijtmans LG., Huynen MA., Holt IJ. michal.minczuk@mrc-mbu.cam.ac.uk Accepted 2021-NOV-26 AT 16:10 GMT AT 16:10 GMT by MGT Nucleic acids research 2011 Only protein-protein interactions imex curation imex curation TEFM (c17orf42) is necessary for transcription of human mtDNA. TEFM (c17orf42) is necessary for transcription of human mtDNA. Nucleic acids research 2011 imex curation Minczuk M., He J., Duch AM., Ettema TJ., Chlebowski A., Dzionek K., Nijtmans LG., Huynen MA., Holt IJ. michal.minczuk@mrc-mbu.cam.ac.uk Only protein-protein interactions imex curation human-293 flp-in Homo sapiens 293 flp-in 293 flp-in pull down pull down western blot western blot Immuno blot Immuno blot Minczuk M., He J., Duch AM., Ettema TJ., Chlebowski A., Dzionek K., Nijtmans LG., Huynen MA., Holt IJ. michal.minczuk@mrc-mbu.cam.ac.uk Accepted 2021-NOV-26 AT 16:10 GMT AT 16:10 GMT by MGT Nucleic acids research 2011 Only protein-protein interactions imex curation imex curation TEFM (c17orf42) is necessary for transcription of human mtDNA. TEFM (c17orf42) is necessary for transcription of human mtDNA. Nucleic acids research 2011 imex curation Minczuk M., He J., Duch AM., Ettema TJ., Chlebowski A., Dzionek K., Nijtmans LG., Huynen MA., Holt IJ. michal.minczuk@mrc-mbu.cam.ac.uk Only protein-protein interactions imex curation human-293 flp-in Homo sapiens 293 flp-in 293 flp-in bn-page blue native page bn-page western blot western blot Immuno blot Immuno blot Minczuk M., He J., Duch AM., Ettema TJ., Chlebowski A., Dzionek K., Nijtmans LG., Huynen MA., Holt IJ. Complex was pre-purified by affinity chromatography michal.minczuk@mrc-mbu.cam.ac.uk Accepted 2021-NOV-26 AT 16:10 GMT AT 16:10 GMT by MGT Nucleic acids research 2011 Only protein-protein interactions imex curation imex curation rm04_human 39S ribosomal protein L4, mitochondrial MRPL4 Mitochondrial large ribosomal subunit protein uL4m CDABP0091 CGI-28 protein protein human Homo sapiens Human MLQFVRAGARAWLRPTGSQGLSSLAEEAARATENPEQVASEGLPEPVLRKVELPVPTHRRPVQAWVESLRGFEQERVGLADLHPDVFATAPRLDILHQVAMWQKNFKRISYAKTKTRAEVRGGGRKPWPQKGTGRARHGSIRSPLWRGGGVAHGPRGPTSYYYMLPMKVRALGLKVALTVKLAQDDLHIMDSLELPTGDPQYLTELAHYRRWGDSVLLVDLTHEEMPQSIVEATSRLKTFNLIPAVGLNVHSMLKHQTLVLTLPTVAFLEDKLLWQDSRYRPLYPFSLPYSDFPRPLPHATQGPAATPYHC 8267C61C42BFCF07 rm39_human 39S ribosomal protein L39, mitochondrial MRPL39 C21orf92 MRPL5 RPML5 39S ribosomal protein L5, mitochondrial MSTP003 PRED22 Mitochondrial large ribosomal subunit protein mL39 protein protein human Homo sapiens Human MEALAMGSRALRLWLVAPGGGIKWRFIATSSASQLSPTELTEMRNDLFNKEKARQLSLTPRTEKIEVKHVGKTDPGTVFVMNKNISTPYSCAMHLSEWYCRKSILALVDGQPWDMYKPLTKSCEIKFLTFKDCDPGEVNKAYWRSCAMMMGCVIERAFKDEYMVNLVRAPEVPVISGAFCYDVVLDSKLDEWMPTKENLRSFTKDAHALIYKDLPFETLEVEAKVALEIFQHSKYKVDFIEEKASQNPERIVKLHRIGDFIDVSEGPLIPRTSICFQYEVSAVHNLQPTQPSLIRRFQGVSLPVHLRAHFTIWDKLLERSRKMVTEDQSKATEECTST BB0139678F72B63D rm13_human 39S ribosomal protein L13, mitochondrial MRPL13 Mitochondrial large ribosomal subunit protein uL13m protein protein human Homo sapiens Human MSSFSRAPQQWATFARIWYLLDGKMQPPGKLAAMASIRLQGLHKPVYHALSDCGDHVVIMNTRHIAFSGNKWEQKVYSSHTGYPGGFRQVTAAQLHLRDPVAIVKLAIYGMLPKNLHRRTMMERLHLFPDEYIPEDILKNLVEELPQPRKIPKRLDEYTQEEIDAFPRLWTPPEDYRL AF4FD524CC234D52 rpom_human DNA-directed RNA polymerase, mitochondrial POLRMT protein protein human Homo sapiens Human MSALCWGRGAAGLKRALRPCGRPGLPGKEGTAGGVCGPRRSSSASPQEQDQDRRKDWGHVELLEVLQARVRQLQAESVSEVVVNRVDVARLPECGSGDGSLQPPRKVQMGAKDATPVPCGRWAKILEKDKRTQQMRMQRLKAKLQMPFQSGEFKALTRRLQVEPRLLSKQMAGCLEDCTRQAPESPWEEQLARLLQEAPGKLSLDVEQAPSGQHSQAQLSGQQQRLLAFFKCCLLTDQLPLAHHLLVVHHGQRQKRKLLTLDMYNAVMLGWARQGAFKELVYVLFMVKDAGLTPDLLSYAAALQCMGRQDQDAGTIERCLEQMSQEGLKLQALFTAVLLSEEDRATVLKAVHKVKPTFSLPPQLPPPVNTSKLLRDVYAKDGRVSYPKLHLPLKTLQCLFEKQLHMELASRVCVVSVEKPTLPSKEVKHARKTLKTLRDQWEKALCRALRETKNRLEREVYEGRFSLYPFLCLLDEREVVRMLLQVLQALPAQGESFTTLARELSARTFSRHVVQRQRVSGQVQALQNHYRKYLCLLASDAEVPEPCLPRQYWEELGAPEALREQPWPLPVQMELGKLLAEMLVQATQMPCSLDKPHRSSRLVPVLYHVYSFRNVQQIGILKPHPAYVQLLEKAAEPTLTFEAVDVPMLCPPLPWTSPHSGAFLLSPTKLMRTVEGATQHQELLETCPPTALHGALDALTQLGNCAWRVNGRVLDLVLQLFQAKGCPQLGVPAPPSEAPQPPEAHLPHSAAPARKAELRRELAHCQKVAREMHSLRAEALYRLSLAQHLRDRVFWLPHNMDFRGRTYPCPPHFNHLGSDVARALLEFAQGRPLGPHGLDWLKIHLVNLTGLKKREPLRKRLAFAEEVMDDILDSADQPLTGRKWWMGAEEPWQTLACCMEVANAVRASDPAAYVSHLPVHQDGSCNGLQHYAALGRDSVGAASVNLEPSDVPQDVYSGVAAQVEVFRRQDAQRGMRVAQVLEGFITRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELSDFPQEFVWEASHYLVRQVFKSLQEMFSGTRAIQHWLTESARLISHMGSVVEWVTPLGVPVIQPYRLDSKVKQIGGGIQSITYTHNGDISRKPNTRKQKNGFPPNFIHSLDSSHMMLTALHCYRKGLTFVSVHDCYWTHAADVSVMNQVCREQFVRLHSEPILQDLSRFLVKRFCSEPQKILEASQLKETLQAVPKPGAFDLEQVKRSTYFFS DIP protein O00411 original sequence version: 113 47AFBF2E0884AEFA dhx30_human ATP-dependent RNA helicase DHX30 DHX30 KIAA0890 DDX30 DEAH box protein 30 protein protein human Homo sapiens Human MFSLDSFRKDRAQHRQRQCKLPPPRLPPMCVNPTPGGTISRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWIAPELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPVGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRKTADD 33D9A08799FE7A02 rt23_human 28S ribosomal protein S23, mitochondrial MRPS23 Mitochondrial small ribosomal subunit protein mS23 CGI-138 HSPC329 protein protein human Homo sapiens Human MAGSRLETVGSIFSRTRDLVRAGVLKEKPLWFDVYDAFPPLREPVFQRPRVRYGKAKAPIQDIWYHEDRIRAKFYSVYGSGQRAFDLFNPNFKSTCQRFVEKYTELQKLGETDEEKLFVETGKALLAEGVILRRVGEARTQHGGSHVSRKSEHLSVRPQTALEENETQKEVPQDQHLEAPADQSKGLLPP 0A50DEDA9BFB74AF rm15_human 39S ribosomal protein L15, mitochondrial MRPL15 Mitochondrial large ribosomal subunit protein uL15m HSPC145 protein protein human Homo sapiens Human MAGPLQGGGARALDLLRGLPRVSLANLKPNPGSKKPERRPRGRRRGRKCGRGHKGERQRGTRPRLGFEGGQTPFYIRIPKYGFNEGHSFRRQYKPLSLNRLQYLIDLGRVDPSQPIDLTQLVNGRGVTIQPLKRDYGVQLVEEGADTFTAKVNIEVQLASELAIAAIEKNGGVVTTAFYDPRSLDIVCKPVPFFLRGQPIPKRMLPPEELVPYYTDAKNRGYLADPAKFPEARLELARKYGYILPDITKDELFKMLCTRKDPRQIFFGLAPGWVVNMADKKILKPTDENLLKYYTS D7396086F808A72C tefm_human Transcription elongation factor, mitochondrial TEFM C17orf42 protein protein human Homo sapiens Human MSGSVLFTAGERWRCFLTPSRSSLYWALHNFCCRKKSTTPKKITPNVTFCDENAKEPENALDKLFSSEQQASILHVLNTASTKELEAFRLLRGRRSINIVEHRENFGPFQNLESLMNVPLFKYKSTVQVCNSILCPKTGREKRKSPENRFLRKLLKPDIERERLKAVNSIISIVFGTRRIAWAHLDRKLTVLDWQQSDRWSLMRGIYSSSVYLEEISSIISKMPKADFYVLEKTGLSIQNSSLFPILLHFHIMEAMLYALLNKTFAQDGQHQVLSMNRNAVGKHFELMIGDSRTSGKELVKQFLFDSILKADPRVFFPSDKIVHYRQMFLSTELQRVEELYDSLLQAIAFYELAVFDSQP 2D3473AC87D2650C rt12_human 28S ribosomal protein S12, mitochondrial MT-RPS12 MRPS12 RPMS12 RPSM12 Mitochondrial small ribosomal subunit protein uS12m protein protein human Homo sapiens Human MSWSGLLHGLNTSLTCGPALVPRLWATCSMATLNQMHRLGPPKRPPRKLGPTEGRPQLKGVVLCTFTRKPKKPNSANRKCCRVRLSTGREAVCFIPGEGHTLQEHQIVLVEGGRTQDLPGVKLTVVRGKYDCGHVQKK 893FD2CA1C7187A7 tfam_human Transcription factor A, mitochondrial TCF6 Mitochondrial transcription factor 1 Transcription factor 6-like 2 TFAM TCF6L2 Transcription factor 6 protein protein human Homo sapiens Human MAFLRSMWGVLSALGRSGAELCTGCGSRLRSPFSFVYLPRWFSSVLASCPKKPVSSYLRFSKEQLPIFKAQNPDAKTTELIRRIAQRWRELPDSKKKIYQDAYRAEWQVYKEEISRFKEQLTPSQIMSLEKEIMDKHLKRKAMTKKKELTLLGKPKRPRSAYNVYVAERFQEAKGDSPQEKLKTVKENWKNLSDSEKELYIQHAKEDETRYHNEMKSWEEQMIEVGRKDLLRRTIKKQRKYGAEEC 81F00CFA12DA4924 tfb2m_human Dimethyladenosine transferase 2, mitochondrial TFB2M NS5ATP5 S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 2 Mitochondrial 12S rRNA dimethylase 2 Mitochondrial transcription factor B2 Hepatitis C virus NS5A-transactivated protein 5 protein protein human Homo sapiens Human MWIPVVGLPRRLRLSALAGAGRFCILGSEAATRKHLPARNHCGLSDSSPQLWPEPDFRNPPRKASKASLDFKRYVTDRRLAETLAQIYLGKPSRPPHLLLECNPGPGILTQALLEAGAKVVALESDKTFIPHLESLGKNLDGKLRVIHCDFFKLDPRSGGVIKPPAMSSRGLFKNLGIEAVPWTADIPLKVVGMFPSRGEKRALWKLAYDLYSCTSIYKFGRIEVNMFIGEKEFQKLMADPGNPDLYHVLSVIWQLACEIKVLHMEPWSSFDIYTRKGPLENPKRRELLDQLQQKLYLIQMIPRQNLFTKNLTPMNYNIFFHLLKHCFGRRSATVIDHLRSLTPLDARDILMQIGKQEDEKVVNMHPQDFKTLFETIERSKDCAYKWLYDETLEDR DIP protein Q9H5Q4 original sequence version: 96 C13DC398974BB88F ptcd3_human Pentatricopeptide repeat domain-containing protein 3, mitochondrial Transformation-related gene 15 protein TRG15 28S ribosomal protein S39, mitochondrial MRPS39 PTCD3 Mitochondrial small ribosomal subunit protein mS39 protein protein human Homo sapiens Human MAVVSAVRWLGLRSRLGQPLTGRRAGLCEQARSCRFYSGSATLSKVEGTDVTGIEEVVIPKKKTWDKVAVLQALASTVNRDTTAVPYVFQDDPYLMPASSLESRSFLLAKKSGENVAKFIINSYPKYFQKDIAEPHIPCLMPEYFEPQIKDISEAALKERIELRKVKASVDMFDQLLQAGTTVSLETTNSLLDLLCYYGDQEPSTDYHFQQTGQSEALEEENDETSRRKAGHQFGVTWRAKNNAERIFSLMPEKNEHSYCTMIRGMVKHRAYEQALNLYTELLNNRLHADVYTFNALIEATVCAINEKFEEKWSKILELLRHMVAQKVKPNLQTFNTILKCLRRFHVFARSPALQVLREMKAIGIEPSLATYHHIIRLFDQPGDPLKRSSFIIYDIMNELMGKRFSPKDPDDDKFFQSAMSICSSLRDLELAYQVHGLLKTGDNWKFIGPDQHRNFYYSKFFDLICLMEQIDVTLKWYEDLIPSAYFPHSQTMIHLLQALDVANRLEVIPKIWKDSKEYGHTFRSDLREEILMLMARDKHPPELQVAFADCAADIKSAYESQPIRQTAQDWPATSLNCIAILFLRAGRTQEAWKMLGLFRKHNKIPRSELLNELMDSAKVSNSPSQAIEVVELASAFSLPICEGLTQRVMSDFAINQEQKEALSNLTALTSDSDTDSSSDSDSDTSEGK 901DBE1FF3A2E0B9 tefm-dhx30-1 2 6 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 7 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 5 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 13 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 11 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 12 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate region strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level 16 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level association association Fig. 5B, Supp. table 2 tefm-dhx30-2 3 12 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate region strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level 16 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level association association Fig. 5C, 6B polrmt-tefm-1 3 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate region strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level 15 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level 12 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level association association Fig. 5G-H tefm-polrmt-1 4 12 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate region strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 16 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level association association SUpp. Fig. 4A Treatment of the mitochondrial lysate with RNase A, but not DNase I, disrupted the complex tefm-polrmt-2 3 12 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate region strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Fig. 6B Cells were pretreated with RNase A tefm-polrmt-3 1 12 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate region strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate C-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 7C