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  <entry>
    <source releaseDate="2022-02-03">
      <names>
        <shortLabel>DIP</shortLabel>
        <fullName>Database of Interacting Proteins</fullName>
      </names>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0465" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="pubmed" dbAc="MI:0446" id="14681454" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1579232" refType="identity" refTypeAc="MI:0356"/>
      </xref>
      <attributeList>
        <attribute name="postaladdress">611 Young Drive East; Los Angeles CA 90095; USA</attribute>
        <attribute name="url" nameAc="MI:0614">http://dip.doe-mbi.ucla.edu/</attribute>
        <attribute name="contact-email" nameAc="MI:0634">dip@mbi.ucla.edu</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Structures of SRP54 and SRP19, the two proteins that organize the ribonucleic core of the signal recognition particle from Pyrococcus furiosus.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="18953414" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-26903505" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="imex" dbAc="MI:0670" id="IM-28904" refType="imex-primary" refTypeAc="MI:0662"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Structures of SRP54 and SRP19, the two proteins that organize the ribonucleic core of the signal recognition particle from Pyrococcus furiosus.</attribute>
            <attribute name="journal" nameAc="MI:0885">PloS one</attribute>
            <attribute name="publication year" nameAc="MI:0886">2007</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Egea PF., Napetschnig J., Walter P., Stroud RM.</attribute>
            <attribute name="contact-email" nameAc="MI:0634">stroud@msg.ucsf.edu</attribute>
            <attribute name="author-announcement">24-May-2021: Contacted by IntAct-Help.</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959">imex curation</attribute>
          </attributeList>
        </bibref>
        <xref>
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        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="186497">
            <names>
              <shortLabel>pyrfu</shortLabel>
              <fullName>Pyrococcus furiosus</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>x-ray diffraction</shortLabel>
            <fullName>x-ray crystallography</fullName>
            <alias type="go synonym" typeAc="MI:0303">X-ray</alias>
            <alias type="synonym" typeAc="MI:1041">X-ray</alias>
            <alias type="synonym" typeAc="MI:1041">x-ray diffraction</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0114" refType="identity" refTypeAc="MI:0356"/>
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          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
            <alias type="synonym" typeAc="MI:1041">predetermined</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
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          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Egea PF., Napetschnig J., Walter P., Stroud RM.</attribute>
          <attribute name="contact-email" nameAc="MI:0634">stroud@msg.ucsf.edu</attribute>
          <attribute name="accepted">Accepted 2021-APR-26 AT 07:33 BST AT 07:33 BST by ORCHARD</attribute>
          <attribute name="journal" nameAc="MI:0885">PloS one</attribute>
          <attribute name="publication year" nameAc="MI:0886">2007</attribute>
          <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>srp54_pyrfu</shortLabel>
          <fullName>Signal recognition particle 54 kDa protein</fullName>
          <alias type="gene name" typeAc="MI:0301">srp54</alias>
          <alias type="locus name" typeAc="MI:0305">PF1731</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="Q8U070" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensemblbacteria" id="AAL81855" version="SP_122" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q977Q0" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-26904515" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensemblbacteria" id="AAL81855" version="SP_122" refType="transcript"/>
          <secondaryRef db="ensemblbacteria" id="PF1731" version="SP_122" refType="gene"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0003924" version="SP_122"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005525" version="SP_122"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0006614" version="SP_122"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0008312" version="SP_122"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0048500" version="SP_122"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="WP_011012877.1"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3DM5"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR003593"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR027417"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR036891"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR013822"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR004125"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR036225"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR022941"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR000897"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR042101"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
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            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="186497">
          <names>
            <shortLabel>pyrfu</shortLabel>
            <fullName>Pyrococcus furiosus</fullName>
          </names>
        </organism>
        <sequence>MVLDNLGKALANTLKKIARASSVDEALIKELVRDIQRALIQADVNVRLVLQLTREIQRRALEEKPPAGISKKEHIIKIVYEELTKFLGTEAKPIEIKEKPTILLMVGIQGSGKTTTVAKLARYFQKRGYKVGVVCSDTWRPGAYHQLRQLLDRYHIEVFGNPQEKDAIKLAKEGVDYFKSKGVDIIIVDTAGRHKEDKALIEEMKQISNVIHPHEVILVIDGTIGQQAYNQALAFKEATPIGSIIVTKLDGSAKGGGALSAVAATGAPIKFIGTGEKIDDIEPFDPPRFVSRLLGLGDIQGLLEKFKELEKEVEIKEEDIERFLRGKFTLKDMYAQLEAMRKMGPLKQILRMIPGLGYSLPDDVISIGEERLKKFKVIMDSMTEEELLNPEIINYSRIKRIARGSGTSTKDVKELLDQYRQMKKLFKSMNKRQLSRLARRFGM</sequence>
        <attributeList>
          <attribute name="crc64">102D1717B207F71A</attribute>
        </attributeList>
      </interactor>
      <interactor id="3">
        <names>
          <shortLabel>srp19_pyrfu</shortLabel>
          <fullName>Signal recognition particle 19 kDa protein</fullName>
          <alias type="gene name" typeAc="MI:0301">srp19</alias>
          <alias type="locus name" typeAc="MI:0305">PF1894</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="Q8TZT9" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensemblbacteria" id="AAL82018" version="SP_94" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-26904487" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensemblbacteria" id="AAL82018" version="SP_94" refType="transcript"/>
          <secondaryRef db="ensemblbacteria" id="PF1894" version="SP_94" refType="gene"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0006614" version="SP_94"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0008312" version="SP_94"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0048500" version="SP_94"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="WP_011013033.1"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3DLU"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3DLV"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR002778"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR036521"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR022938"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
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            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="186497">
          <names>
            <shortLabel>pyrfu</shortLabel>
            <fullName>Pyrococcus furiosus</fullName>
          </names>
        </organism>
        <sequence>MGRFVVWPSELDSRLSRKYGRIVPRSIAVESPRVEEIVRAAEELKFKVIRVEEDKLNPRLSGIDEELRTFGMIVLESPYGKSKSLKLIAQKIREFRRRSA</sequence>
        <attributeList>
          <attribute name="crc64">648EF600402C9CD6</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="4" imexId="IM-28904-1">
        <names>
          <shortLabel>srp19-1</shortLabel>
        </names>
        <xref>
          <primaryRef db="wwpdb" dbAc="MI:0805" id="3DLU" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="wwpdb" dbAc="MI:0805" id="3DLV" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-26904485" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-28904-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="5">
            <interactorRef>3</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <experimentalPreparationList>
              <experimentalPreparation>
                <names>
                  <shortLabel>purified</shortLabel>
                  <fullName>purified</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0350" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537811" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalPreparation>
            </experimentalPreparationList>
            <featureList>
              <feature id="6">
                <names>
                  <shortLabel>binding region</shortLabel>
                  <fullName>binding region</fullName>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-26904511" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>binding region</shortLabel>
                    <fullName>binding-associated region</fullName>
                    <alias type="synonym" typeAc="MI:1041">binding region</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0117" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-456493" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <begin position="1"/>
                    <endStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
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                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
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                      </xref>
                    </endStatus>
                    <end position="100"/>
                  </featureRange>
                </featureRangeList>
              </feature>
            </featureList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="83333">
                <names>
                  <shortLabel>ecoli</shortLabel>
                  <fullName>Escherichia coli (strain K12)</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
            <stoichiometry value="4"/>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>direct interaction</shortLabel>
            <fullName>direct interaction</fullName>
          </names>
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            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">Fig 7, Table 1</attribute>
        </attributeList>
      </interaction>
      <interaction id="7" imexId="IM-28904-3">
        <names>
          <shortLabel>srp54-1</shortLabel>
        </names>
        <xref>
          <primaryRef db="wwpdb" dbAc="MI:0805" id="3DM5" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-26904513" refType="identity" refTypeAc="MI:0356"/>
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        <participantList>
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            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
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            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
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                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
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              </experimentalRole>
            </experimentalRoleList>
            <experimentalPreparationList>
              <experimentalPreparation>
                <names>
                  <shortLabel>purified</shortLabel>
                  <fullName>purified</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0350" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537811" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalPreparation>
            </experimentalPreparationList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="83333">
                <names>
                  <shortLabel>ecoli</shortLabel>
                  <fullName>Escherichia coli (strain K12)</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
            <stoichiometry value="4"/>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>direct interaction</shortLabel>
            <fullName>direct interaction</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0407" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608833" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">Fig 2, Table 1</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>