UniProt UniProt http://www.uniprot.org Molecular interaction of neutral trehalase with other enzymes of trehalose metabolism in the fission yeast Schizosaccharomyces pombe. Molecular interaction of neutral trehalase with other enzymes of trehalose metabolism in the fission yeast Schizosaccharomyces pombe. European journal of biochemistry 2002 imex curation Soto T., Franco A., Padmanabhan S., Vicente-Soler J., Cansado J., Gacto M., Soto T., Franco A., Padmanabhan S., Vicente-Soler J., Cansado J., Gacto M. jcansado@um.es 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight identificat confirmation by molecular weight weight identificat Soto T., Franco A., Padmanabhan S., Vicente-Soler J., Cansado J., Gacto M., Soto T., Franco A., Padmanabhan S., Vicente-Soler J., Cansado J., Gacto M. jcansado@um.es Accepted 2021-MAR-08 AT 05:32 GMT AT 05:32 GMT by KALPANAP European journal of biochemistry 2002 Only protein-protein interactions imex curation imex curation Molecular interaction of neutral trehalase with other enzymes of trehalose metabolism in the fission yeast Schizosaccharomyces pombe. Molecular interaction of neutral trehalase with other enzymes of trehalose metabolism in the fission yeast Schizosaccharomyces pombe. European journal of biochemistry 2002 imex curation Soto T., Franco A., Padmanabhan S., Vicente-Soler J., Cansado J., Gacto M., Soto T., Franco A., Padmanabhan S., Vicente-Soler J., Cansado J., Gacto M. jcansado@um.es 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast pull down pull down anti tag western anti tag western blot anti tag western Soto T., Franco A., Padmanabhan S., Vicente-Soler J., Cansado J., Gacto M., Soto T., Franco A., Padmanabhan S., Vicente-Soler J., Cansado J., Gacto M. jcansado@um.es Accepted 2021-MAR-08 AT 05:32 GMT AT 05:32 GMT by KALPANAP European journal of biochemistry 2002 Only protein-protein interactions imex curation imex curation Molecular interaction of neutral trehalase with other enzymes of trehalose metabolism in the fission yeast Schizosaccharomyces pombe. Molecular interaction of neutral trehalase with other enzymes of trehalose metabolism in the fission yeast Schizosaccharomyces pombe. European journal of biochemistry 2002 imex curation Soto T., Franco A., Padmanabhan S., Vicente-Soler J., Cansado J., Gacto M., Soto T., Franco A., Padmanabhan S., Vicente-Soler J., Cansado J., Gacto M. jcansado@um.es 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Soto T., Franco A., Padmanabhan S., Vicente-Soler J., Cansado J., Gacto M., Soto T., Franco A., Padmanabhan S., Vicente-Soler J., Cansado J., Gacto M. jcansado@um.es Accepted 2021-MAR-08 AT 05:32 GMT AT 05:32 GMT by KALPANAP European journal of biochemistry 2002 Only protein-protein interactions imex curation imex curation trea_schpo Cytosolic neutral trehalase Neutral trehalase SPBC660.07 Alpha,alpha-trehalase Alpha,alpha-trehalose glucohydrolase ntp1 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MPSKFSSKYVDTEAISNDDDNPFATAKSYYSKDTDLSTRVSAGRPRTLSTSMEASAAPTIPELKNLRRRGSLDEHKQPRKFLVDVDKTLNALLESEDTDRNMQITIEDTGPKVVSLGSASSGGYRLYELRGTYQLSNLLQELTLAKDYGRRYILLDERRLNENPVNRLSRLIKGTFWDALTRRIDASVLDVICRDTKDRSGSHVNRIYVPKAEQEMYEYYVRAAKERPYLNLQVEYLPEEITPEWVRDVNDKPGLLALAMEKYQDDEGNTHLRGVPFVVPGGRFNELYGWDSYFESLGLLVDDRVDLAKGMVENFIFEITYYGKILNANRTYYLLRSQPPFLTDMALRVYERIKNEEGSLDFLHRAFSATIKEYHTVWTATPRLDPKTGLSRYRPGGLGIPPETEASHFEHLLRPYMEKYHMTLEEFTHAYNYQQIHEPALDEYFVHDRAVRESGHDTTYRLEKVCADLATVDLNSLLYKYETDISHVILEYFDDKFVLPNGTIETSAIWDRRARARRAAMEKYLWSEADSMWYDYNTKLETKSTYESATAFWALWAGVATPRQAAKFVDVSLPKFEVAGGIVAGTKRSLGKVGLDNPSRQWDYPNGWSPQQILAWYGLIRYGYEEETRRLVYRWLYTITKSFVDFNGIVVEKYDLTRPVDPHRVEAEYGNQGVNIKGVAREGFGWVNASYEVGLTFCNSHMRRALGACTTPDVFFAGIKEESLPAFENLSIHKN 73FF979534E91BD4 tps1_schpo Alpha,alpha-trehalose-phosphate synthase [UDP-forming] Trehalose-6-phosphate synthase UDP-glucose-glucosephosphate glucosyltransferase SPAC328.03 tps1 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSDAHDTIKSLTGDASNSRRLIVVSNRLPITIKRKDNGTYDFSMSSGGLVSALSGLKKLMTFQWLGWCGQEIPEDEKPMIIQRLQDECSAIPVFLDDETADRHYNGFSNSILWPLFHYHPGEINFDEENWEAYRAANYAFAEAIVKNLQDGDLIWVQDYHLMVLPQMLRELIGDKFKDIKIGFFLHTPFPSSEIYRVLPVRNEILEGVLNCDLVGFHTYDYARHFLSACSRILNLSTLPNGVEYNGQMVSVGTFPIGIDPEKFSDALKSDVVKDRIASIERRLQGVKVIVGVDRLDYIKGVPQKFHAFEVFLEQYPEWVGKVVLVQVAVPSRQDVEEYQNLRAVVNELVGRINGRFGTVEYTPIHFLHKSVRFEELVALYNVSDVCLITSTRDGMNLVSYEYICTQQERHGALILSEFAGAAQSLNGSIVINPWNTEELANSIHDALTMPEKQREANENKLFRYVNKYTSQFWGQSFVGELQRIQHYSHPHPRRTNPILRTKSAQVLSMNSSS 9FC247B626CE2B00 tpp1_schpo Trehalose-phosphatase Trehalose-6-phosphate phosphatase SPAC19G12.15c tpp1 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSVYGKIPSTSFEHENTFELSGDLLDPEELKSLGVSGRIIYVLRHLPFKSSINEETREWDLSGRRGATTMYSSMNWLANSTYWQTTLVGWTGVIPTVSEKEENKDAVTRLDSQDVKRFEETYSQWNSGERSTEYVPVWLPGPEKGSETIINETRSQQSRWLAYAENVIRPLIHYKYWPSSEVDENEEQWWRDYVKMNHAFADKICEIYKPGDFIIVQDYSLFLVPQLIRNKIDDAVIGFYHHHPFPSSEIARCFPRRRAILRSVLGADFIGFEDYSYARHFISCCSRVLDLEIGHDWVNLNGNKVTVRAITVGIDVPRIIRSSGNVSVSEKLEELNKRYENMKVILGRDRLDELYGVPQKLRSFQRFLRTYPEWRKKVVLIQITISSAFKHPKLLSSIKKLVQAINQEFGTDDYTPVHHVEEQLEPADYFALLTRADALFINSIREGVSNLALEYVVCQRDRYGMVLLSEFTATSAMLHDVPLINPWDYNECAEIISNALSTPLERRKMIERESYKQVTTHTMQSWTSSLIRSLANKLAATKTDQRIPTLTPEHALSVYSKASKRLFMMDYDGTLTPIVRDPNAAVPSKKLLDNLATLAADPKNQVWIISGRDQQFLRNWMDDIKGLGLSAEHGSFVRKPHSTTWINLAELLDMSWKKEVRRIFQYYTDRTQGSSIEEKRCAMTWHYRKADPENGAFQALECEALLEELVCSKYDVEIMRGKANLEVRPSSINKGGIVKQILSSYPEDSLPSFIFCAGDDRTDEDMFRSLHKNTRINKETSFAVTIGSDKKLSIADWCIADPANVIDILADLANFTN 23CFD2533505CCBA tps1-ntp1-1 2 5 unspecified role unspecified role bait bait purified purified c-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 1 Interaction observed in both control, exponentially growing cells and both thermal and osmotic stressed cells. tps1-ntp1-2 3 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate c-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 2A ntp1-tps1-1 3 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate c-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 2A tps1-tps1-3 2 5 unspecified role unspecified role bait bait purified purified c-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 3B Interaction observed in both control, exponentially growing cells and both thermal ands osmotic stressed cells. ntp1-ntp1-1 2 4 unspecified role unspecified role bait bait purified purified c-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 3A Interaction observed in both control, exponentially growing cells and both thermal ands osmotic stressed cells. tps1-tpp1-1 2 5 unspecified role unspecified role bait bait purified purified c-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 4A Interaction observed in both control, exponentially growing cells and both thermal ands osmotic stressed cells. ntp1-tpp1-1 2 4 unspecified role unspecified role bait bait purified purified c-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 4B Interaction observed in both control, exponentially growing cells and both thermal ands osmotic stressed cells. ntp1-tps1-2 1 6 weight identificat confirmation by molecular weight weight identificat anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed cell lysate cell lysate c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 5 weight identificat confirmation by molecular weight weight identificat anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed cell lysate cell lysate c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 weight identificat confirmation by molecular weight weight identificat anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed cell lysate cell lysate c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 5 Interaction observed in both control, exponentially growing cells and both thermal ands osmotic stressed cells.