IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Dalbavancin binds ACE2 to block its interaction with SARS-CoV-2 spike protein and is effective in inhibiting SARS-CoV-2 infection in animal models. Dalbavancin binds ACE2 to block its interaction with SARS-CoV-2 spike protein and is effective in inhibiting SARS-CoV-2 infection in animal models. Cell research 2020 imex curation Wang G., Yang ML., Duan ZL., Liu FL., Jin L., Long CB., Zhang M., Tang XP., Xu L., Li YC., Kamau PM., Yang L., Liu HQ., Xu JW., Chen JK., Zheng YT., Peng XZ., Lai R. I cannot find any info regarding the Spike protein;presumably "purified". zhengyt@mail.kiz.ac.cn,pengxiaozhong@pumc.edu.cn,rlai@mail.kiz.ac.cn 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Wang G., Yang ML., Duan ZL., Liu FL., Jin L., Long CB., Zhang M., Tang XP., Xu L., Li YC., Kamau PM., Yang L., Liu HQ., Xu JW., Chen JK., Zheng YT., Peng XZ., Lai R. I cannot find any info regarding the Spike protein;presumably "purified". zhengyt@mail.kiz.ac.cn,pengxiaozhong@pumc.edu.cn,rlai@mail.kiz.ac.cn Accepted 2021-JAN-05 AT 08:41 GMT AT 08:41 GMT by ORCHARD Cell research 2020 Only protein-protein interactions imex curation imex curation Dalbavancin binds ACE2 to block its interaction with SARS-CoV-2 spike protein and is effective in inhibiting SARS-CoV-2 infection in animal models. Dalbavancin binds ACE2 to block its interaction with SARS-CoV-2 spike protein and is effective in inhibiting SARS-CoV-2 infection in animal models. Cell research 2020 imex curation Wang G., Yang ML., Duan ZL., Liu FL., Jin L., Long CB., Zhang M., Tang XP., Xu L., Li YC., Kamau PM., Yang L., Liu HQ., Xu JW., Chen JK., Zheng YT., Peng XZ., Lai R. I cannot find any info regarding the Spike protein;presumably "purified". zhengyt@mail.kiz.ac.cn,pengxiaozhong@pumc.edu.cn,rlai@mail.kiz.ac.cn 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Wang G., Yang ML., Duan ZL., Liu FL., Jin L., Long CB., Zhang M., Tang XP., Xu L., Li YC., Kamau PM., Yang L., Liu HQ., Xu JW., Chen JK., Zheng YT., Peng XZ., Lai R. I cannot find any info regarding the Spike protein;presumably "purified". zhengyt@mail.kiz.ac.cn,pengxiaozhong@pumc.edu.cn,rlai@mail.kiz.ac.cn Accepted 2021-JAN-05 AT 08:41 GMT AT 08:41 GMT by ORCHARD Cell research 2020 Only protein-protein interactions imex curation imex curation Dalbavancin binds ACE2 to block its interaction with SARS-CoV-2 spike protein and is effective in inhibiting SARS-CoV-2 infection in animal models. Dalbavancin binds ACE2 to block its interaction with SARS-CoV-2 spike protein and is effective in inhibiting SARS-CoV-2 infection in animal models. Cell research 2020 imex curation Wang G., Yang ML., Duan ZL., Liu FL., Jin L., Long CB., Zhang M., Tang XP., Xu L., Li YC., Kamau PM., Yang L., Liu HQ., Xu JW., Chen JK., Zheng YT., Peng XZ., Lai R. I cannot find any info regarding the Spike protein;presumably "purified". zhengyt@mail.kiz.ac.cn,pengxiaozhong@pumc.edu.cn,rlai@mail.kiz.ac.cn 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang G., Yang ML., Duan ZL., Liu FL., Jin L., Long CB., Zhang M., Tang XP., Xu L., Li YC., Kamau PM., Yang L., Liu HQ., Xu JW., Chen JK., Zheng YT., Peng XZ., Lai R. I cannot find any info regarding the Spike protein;presumably "purified". zhengyt@mail.kiz.ac.cn,pengxiaozhong@pumc.edu.cn,rlai@mail.kiz.ac.cn Accepted 2021-JAN-05 AT 08:41 GMT AT 08:41 GMT by ORCHARD Cell research 2020 Only protein-protein interactions imex curation imex curation Dalbavancin binds ACE2 to block its interaction with SARS-CoV-2 spike protein and is effective in inhibiting SARS-CoV-2 infection in animal models. Dalbavancin binds ACE2 to block its interaction with SARS-CoV-2 spike protein and is effective in inhibiting SARS-CoV-2 infection in animal models. Cell research 2020 imex curation Wang G., Yang ML., Duan ZL., Liu FL., Jin L., Long CB., Zhang M., Tang XP., Xu L., Li YC., Kamau PM., Yang L., Liu HQ., Xu JW., Chen JK., Zheng YT., Peng XZ., Lai R. I cannot find any info regarding the Spike protein;presumably "purified". zhengyt@mail.kiz.ac.cn,pengxiaozhong@pumc.edu.cn,rlai@mail.kiz.ac.cn 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip sequence tag sequence tag identification sequence tag Wang G., Yang ML., Duan ZL., Liu FL., Jin L., Long CB., Zhang M., Tang XP., Xu L., Li YC., Kamau PM., Yang L., Liu HQ., Xu JW., Chen JK., Zheng YT., Peng XZ., Lai R. I cannot find any info regarding the Spike protein;presumably "purified". zhengyt@mail.kiz.ac.cn,pengxiaozhong@pumc.edu.cn,rlai@mail.kiz.ac.cn Accepted 2021-JAN-05 AT 08:41 GMT AT 08:41 GMT by ORCHARD Cell research 2020 Only protein-protein interactions imex curation imex curation ace2_human Angiotensin-converting enzyme 2 ACE2 UNQ868/PRO1885 Angiotensin-converting enzyme homolog Metalloprotease MPROT15 Angiotensin-converting enzyme-related carboxypeptidase protein protein human Homo sapiens Human MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSIWLIVFGVVMGVIVVGIVILIFTGIRDRKKKNKARSGENPYASIDISKGENNPGFQNTDDVQTSF mint Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses 8EE6EB0A931550E8 dalbavancin dalbavancin BI 397 BI397 dalbavancin dalbavancin B0 small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) glutathione disulfid glutathione disulfide Glutathione disulfide glutathione disulphide GSSG oxidised glutathione Oxidized glutathione OXIDIZED GLUTATHIONE DISULFIDE Oxiglutatione (2S,2'S)-5,5'-[disulfanediylbis({(2R)-3-[(carboxymethyl)amino]-3-oxopropane-1,2-diyl}imino)]bis(2-amino-5-oxopentanoic acid) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) YPZRWBKMTBYPTK-BJDJZHNGSA-N YPZRWBKMTBYPTK-BJDJZHNGSA-N spike_sars2 Spike glycoprotein S E2 Peplomer protein 2 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT s-ace2-48 3 5 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9inse 9inse 8 unspecified role unspecified role bait bait purified purified direct interaction direct interaction 2A s-ace2-49 3 5 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9inse 9inse 6 inhibitor inhibitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role bait bait purified purified direct interaction direct interaction 2A, top s-ace2-50 3 5 competitor competitor competitor unspecified role unspecified role purified purified C-terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 5 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9inse 9inse 8 unspecified role unspecified role bait bait purified purified direct interaction direct interaction 2A, bottom ace2-s-72 1 5 unspecified role unspecified role bait bait purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9inse 9inse 8 unspecified role unspecified role prey prey purified purified direct interaction direct interaction 2B s-ace2-51 1 5 competitor competitor competitor unspecified role unspecified role purified purified C-terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 5 anti tag western anti tag western blot anti tag western antibody detection identification by antibody antibody detection unspecified role unspecified role bait bait purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9inse 9inse 8 unspecified role unspecified role prey prey purified purified direct interaction direct interaction 2B ace2-s-75 1 5 unspecified role unspecified role bait bait purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9inse 9inse 6 inhibitor inhibitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role prey prey purified purified direct interaction direct interaction 2B ace2-s-76 1 5 unspecified role unspecified role bait bait purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9inse 9inse 7 inhibitor inhibitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role prey prey purified purified direct interaction direct interaction 2B ace2-s-77 2 5 unspecified role unspecified role bait bait purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9inse 9inse 8 unspecified role unspecified role prey prey purified purified direct interaction direct interaction 2C ace2-s-78 2 5 unspecified role unspecified role bait bait purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9inse 9inse 6 unspecified role unspecified role prey prey chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 2C ace2-dalbavancin-1 4 5 unspecified role unspecified role bait bait purified purified Q9BYF1:p.Leu391Gly mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L G Q9BYF1:p.Arg393Gly mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R G Q9BYF1:p.Asp355Gly mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain D G Q9BYF1:p.Phe356Gly mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain F G Q9BYF1:p.Phe390Gly mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain F G Q9BYF1:p.Trp48Gly mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain W G Q9BYF1:p.Thr324Gly mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T G Q9BYF1:p.Tyr41Gly mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Y G Q9BYF1:p.Gln325Gly mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Q G Q9BYF1:p.Trp349Gly mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain W G Q9BYF1:p.Lys353Gly mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K G human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 6 unspecified role unspecified role prey prey chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 3F