UniProt UniProt http://www.uniprot.org Molecular insights into the human CLC-7/Ostm1 transporter. Molecular insights into the human CLC-7/Ostm1 transporter. Science advances 2020 imex curation Zhang S., Liu Y., Zhang B., Zhou J., Li T., Liu Z., Li Y., Yang M. maojunyang@tsinghua.edu.cn 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 3d-em 3D electron microscopy 3D-EM electron tomog structure determination identification by structure determination identification by structure determination structure determination Zhang S., Liu Y., Zhang B., Zhou J., Li T., Liu Z., Li Y., Yang M. maojunyang@tsinghua.edu.cn Accepted 2020-NOV-26 AT 13:54 GMT AT 13:54 GMT by MGT Science advances 2020 Only protein-protein interactions imex curation imex curation Molecular insights into the human CLC-7/Ostm1 transporter. Molecular insights into the human CLC-7/Ostm1 transporter. Science advances 2020 imex curation Zhang S., Liu Y., Zhang B., Zhou J., Li T., Liu Z., Li Y., Yang M. maojunyang@tsinghua.edu.cn 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Zhang S., Liu Y., Zhang B., Zhou J., Li T., Liu Z., Li Y., Yang M. maojunyang@tsinghua.edu.cn Accepted 2020-NOV-26 AT 13:54 GMT AT 13:54 GMT by MGT Science advances 2020 Only protein-protein interactions imex curation imex curation clcn7_human H(+)/Cl(-) exchange transporter 7 CLCN7 Chloride channel protein 7 Chloride channel 7 alpha subunit protein protein human Homo sapiens Human MANVSKKVSWSGRDRDDEEAAPLLRRTARPGGGTPLLNGAGPGAARQSPRSALFRVGHMSSVELDDELLDPDMDPPHPFPKEIPHNEKLLSLKYESLDYDNSENQLFLEEERRINHTAFRTVEIKRWVICALIGILTGLVACFIDIVVENLAGLKYRVIKGNIDKFTEKGGLSFSLLLWATLNAAFVLVGSVIVAFIEPVAAGSGIPQIKCFLNGVKIPHVVRLKTLVIKVSGVILSVVGGLAVGKEGPMIHSGSVIAAGISQGRSTSLKRDFKIFEYFRRDTEKRDFVSAGAAAGVSAAFGAPVGGVLFSLEEGASFWNQFLTWRIFFASMISTFTLNFVLSIYHGNMWDLSSPGLINFGRFDSEKMAYTIHEIPVFIAMGVVGGVLGAVFNALNYWLTMFRIRYIHRPCLQVIEAVLVAAVTATVAFVLIYSSRDCQPLQGGSMSYPLQLFCADGEYNSMAAAFFNTPEKSVVSLFHDPPGSYNPLTLGLFTLVYFFLACWTYGLTVSAGVFIPSLLIGAAWGRLFGISLSYLTGAAIWADPGKYALMGAAAQLGGIVRMTLSLTVIMMEATSNVTYGFPIMLVLMTAKIVGDVFIEGLYDMHIQLQSVPFLHWEAPVTSHSLTAREVMSTPVTCLRRREKVGVIVDVLSDTASNHNGFPVVEHADDTQPARLQGLILRSQLIVLLKHKVFVERSNLGLVQRRLRLKDFRDAYPRFPPIQSIHVSQDERECTMDLSEFMNPSPYTVPQEASLPRVFKLFRALGLRHLVVVDNRNQVVGLVTRKDLARYRLGKRGLEELSLAQT E56BC0B4ADE1C695 ostm1_human Osteopetrosis-associated transmembrane protein 1 OSTM1 Chloride channel 7 beta subunit GL HSPC019 UNQ6098/PRO21201 protein protein human Homo sapiens Human MEPGPTAAQRRCSLPPWLPLGLLLWSGLALGALPFGSSPHRVFHDLLSEQQLLEVEDLSLSLLQGGGLGPLSLPPDLPDLDPECRELLLDFANSSAELTGCLVRSARPVRLCQTCYPLFQQVVSKMDNISRAAGNTSESQSCARSLLMADRMQIVVILSEFFNTTWQEANCANCLTNNSEELSNSTVYFLNLFNHTLTCFEHNLQGNAHSLLQTKNYSEVCKNCREAYKTLSSLYSEMQKMNELENKAEPGTHLCIDVEDAMNITRKLWSRTFNCSVPCSDTVPVIAVSVFILFLPVVFYLSSFLHSEQKKRKLILPKRLKSSTSFANIQENSN 108719C2FDC5853D clcn7-ostm1-4 1 3 unspecified role unspecified role neutral component neutral component purified purified c-terminal strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction Supp. Table 1 clcn7-ostm1-2 2 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c-terminal strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q86WC4:p.Tyr300Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y A physical association physical association Fig.2E The CLC-7 E416A mutation resulted in a remarkably reduced protein level compared with wild-type (WT). ostm1-clcn7-7 2 3 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed c-terminal strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q86WC4:p.Tyr300Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y A physical association physical association Fig.2E The CLC-7 E416A mutation resulted in a remarkably reduced protein level compared with wild-type (WT).