DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu NELL2-mediated lumicrine signaling through OVCH2 is required for male fertility. NELL2-mediated lumicrine signaling through OVCH2 is required for male fertility. Science (New York, N.Y.) 2020 imex curation Kiyozumi D., Noda T., Yamaguchi R., Tobita T., Matsumura T., Shimada K., Kodani M., Kohda T., Fujihara Y., Ozawa M., Yu Z., Miklossy G., Bohren KM., Horie M., Okabe M., Matzuk MM., Ikawa M. mmatzuk@bcm.edu, ikawa@biken.osaka-u.ac.jp 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down experimental particp experimental participant identification experimental particp Kiyozumi D., Noda T., Yamaguchi R., Tobita T., Matsumura T., Shimada K., Kodani M., Kohda T., Fujihara Y., Ozawa M., Yu Z., Miklossy G., Bohren KM., Horie M., Okabe M., Matzuk MM., Ikawa M. Info provided by the authors:- >NELL2 is a full length (819aa) one >derived from mouse. 6xHis tag was >added immediately after its C-terminus (no linker sequence). >ROS1 is a full length (2340aa) one >derived from mouse. A FLAG >(DYKDDDDK) epitope tag sequence was >added immediately after its C-terminus. mmatzuk@bcm.edu, ikawa@biken.osaka-u.ac.jp Accepted 2020-JUL-07 AT 09:37 BST AT 09:37 BST by MGT Science (New York, N.Y.) 2020 Only protein-protein interactions imex curation imex curation ros1_mouse Proto-oncogene tyrosine-protein kinase ROS Ros1 Ros-1 Proto-oncogene c-Ros Proto-oncogene c-Ros-1 Receptor tyrosine kinase c-ros oncogene 1 Ros c-Ros receptor tyrosine kinase protein protein mouse Mus musculus Mouse MKNICWLTLKLVKFVVLGCIIWISVAQSTVLSSCLTSCVTNLGRQLDSGTRYNLSEACIHGCQFWNSVDQETCALKCNDTYATICERESCEVGCSNAEGSYEEEVLESTELPTAPFASSIGSHGVTLRWNPANISGVKYIIQWKYAQLPGSWTFTETVSKLSYTVEPLHPFTEYIFRVVWIFTAQLHLYSPPSPSYRTHPYGVPETAPLILNMESWSPDTVEVSWAPPHFPGGPILGYNLRLISKNQKLDSGTQRTSFQFYSTLPNTTYRFSIAAVNEVGEGPEAESTVTTPSPSVQEEEQWLFLSRKTSLRKRSLKYLVDEAHCLWSDAIHHNITGISVYAQQQVVYFSEGTVIWMKGAANMSDVSDLRIFYQGSGLVSSISIDWLYQRMYFIMDKLVYVCELKNCSNLEEITPFSLIAPQKVVVDSYNGYLFYLLRDGIYRVNLPLPSGRDTKAVRIVESGTLKDFAVKPQSKRIIYFNDTMQLFMSTFLDGSAFHRVLPWVPLVTVKSFACENNDFLITDGKAIFQQDSLSFNEFIVGCDLSHIEEFGFGNLVIFGSSVQSYPLPGHPQEVSVLFGSREALIQWTPPALAIGASPSAWQNWTYEVKVYSQDILEITQVFSNISGTMLNVPELQSSTKYTVSVRASSPKGPGPWSAPSVGTTLVPATEPPFIMAVKEDGLWSKPLCSFGPGEFLSSDVGNVSDMDWYNNSLYYSDTKGNVYVRPLNGMDISENYHIPSIVGAGALAFEWLGHFLYWAGKTYVIQRQSVLTGHTDIVTHVKLLVNDMAVDSVGGYLYWTTLYSVESTRLNGESSLVLQAQPWLSGKKVIALTLDLSDGLLYWLVQDNQCIHLYTAVLRGWSGGDATITEFAAWSTSEISQNALMYYSGRLFWINGFRIITAQEIGQRTSVSVSEPAKFNQFTIIQTSLKPLPGNFSSTPKVIPDPVQESSFRIEGHTSSFQILWNEPPAVDWGIVFYSVEFSTHSKFLIIEQQSLPIFTVEGLEPYTLFNLSVTPYTYWGKGQKTSLSFRAPESVPSAPENPRIFILSSGRYTKKNEVVVEFRWNKPKHENGVLTKFEIFYHISKQSGTNRSTEDWMSASVIPPVMSFQLEAVSPEYTVAFQVRVFTSKGPGPFSDIVMSKTSEIKPCPYLISLLGNKIVFLDMDQNQVLWTFSLEGDVSTVGYTTDDEMGYFAQGDTLFLLNLRNHSSSKLFQDALVSDIRVIAVDWIARHLYFALKASQNGTQIFNVDLEHKVKSPREVKTCKAHTTIISFSIYPLLSRLYWTEVSDLGHQMFYCNISNHTSQHVLQPKASNQHGRSQCSCNVTESELSGAMTVDTSDPDRPWIYFTKRQEIWAMDLEGCQCWKVIMVPTIPGKRIISLTVDGEFIYWIMKTKDDAQIYQAKKGSGAILSQVKASRSKHILAYSSALQPFPDKAYLSLASDMVEATILYATNTSLTLKLPPVKTNLTWHGITHPTSTYLIYYMEANRANSSDRRHKMLESQENVARIEGLQPFSMYMIQIAVKNYYSEPLEHLPLGKEIQGQTKSGVPGAVCHINATVLSDTSLHVFWTESHKPNGPKESVRYQLVMSYLAPIPETPLRQGEFPSAKLSLLITKLSGGQLYVMKVLACHPEEMWCTESHPVSVNMFDTPEKPSALVPENTSLQLDWKARSNVNLTGFWFELQKWKYNEFYHVKASCSQGPVYVCNITDLQPYTSYNIRVVVVYTTGENSSSIPESFKTKAGVPSKPGIPKLLEGSKNSIQWEKAEDNGSRLMYYTLEVRKGISNDSQNQSSRWKVVFNGSCSSICTWRSKNLKGTFQFRAVAANEIGLGEYSEISEDITLVEDGVWITETSFILTIIVGIFLVATVPLTFVWHRSLKSHKASKEGLSVLNDNDKELAELRGLAAGVGLANACYAVHTVPTQEEIENLPAFPREKLSLRLLLGSGAFGEVYEGTAIDILGVGSGEIKVAVKTLKKGSTDQEKIEFLKEAHLMSKFNHPNILKQLGVCLLGEPQYIILELMEGGDLLSYLRKARGTTFHGPSLTLLDLVELCVDISKGCVYLEQMHFIHRDLAARNCLVSVKDYTSPRVVKIGDFGLAREIYKNDYYRKRGEGLLPVRWMAPENLMDGIFTSQSDVWSFGILVWEILTLGHQPYPAHSNLDVLNYVQAGGRLEPPRNCPDDLWNLMSQCWAQEPDQRPTFHNIQNQLQLFRNVFLNNVSHCGEAAPTGGVINKGFEGEDDEMVTLNSDDTMPVALMETKNQEGLNYMVLATKCSQGEGSYEGPLGPKELGSCDLKKDKKQPQADKDFCQEPQVAYGSPGLSEGLNYACLAHSEHGDVSE mint This is probably a cell growth or differentiation factor receptor with a tyrosine-protein kinase activity A3A670B0C4151D7C nell2_mouse Protein kinase C-binding protein NELL2 Nell2 MEL91 protein Mel91 NEL-like protein 2 protein protein mouse Mus musculus Mouse MHAMESRVLLRTFCVILGLGAVWGLGVDPSLQIDVLTELELGESTDGVRQVPGLHNGTKAFLFQESPRSIKASTATAERFFQKLRNKHEFTILVTLKQIHLNSGVILSIHHLDHRYLELESSGHRNEIRLHYRSGTHRPHTEVFPYILADAKWHKLSLAFSASHLILHIDCNKIYERVVEMPSTDLPLGTTFWLGQRNNAHGYFKGIMQDVHVLVMPQGFIAQCPDLNRTCPTCNDFHGLVQKIMELQDILSKTSAKLSRAEQRMNRLDQCYCERTCTVKGTTYRESESWTDGCKNCTCLNGTIQCETLVCPAPDCPPKSAPAYVDGKCCKECKSTCQFQGRSYFEGERNTVYSSSGMCVLYECKDQTMKLVENIGCPPLDCPESHQIALSHSCCKVCKGYDFCSEKHTCMENSVCRNLNDRAVCSCRDGFRALREDNAYCEDIDECAEGRHYCRENTMCVNTPGSFMCICKTGYIRIDDYSCTEHDECLTNQHNCDENALCFNTVGGHNCVCKPGYTGNGTTCKAFCKDGCRNGGACIAANVCACPQGFTGPSCETDIDECSEGFVQCDSRANCINLPGWYHCECRDGYHDNGMFAPGGESCEDIDECGTGRHSCTNDTICFNLDGGYDCRCPHGKNCTGDCVHEGKVKHTGQIWVLENDRCSVCSCQTGFVMCRRMVCDCENPTVDLSCCPECDPRLSSQCLHQNGETVYNSGDTWVQDCRQCRCLQGEVDCWPLACPEVECEFSVLPENECCPRCVTDPCQADTIRNDITKTCLDEMNVVRFTGSSWIKHGTECTLCQCKNGHLCCSVDPQCLQEL nell2_mouse-ros1-1 1 2 anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate FLAG Tag FLAG Tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 3 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait purified purified His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f physical association physical association FIg1a