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  <entry>
    <source releaseDate="2022-02-04">
      <names>
        <shortLabel>MINT</shortLabel>
        <fullName>MINT, Dpt of Biology, University of Rome Tor Vergata</fullName>
        <alias type="synonym" typeAc="MI:1041">MINT</alias>
      </names>
      <bibref>
        <xref>
          <primaryRef db="pubmed" dbAc="MI:0446" id="17135203" refType="primary-reference" refTypeAc="MI:0358"/>
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        </xref>
      </bibref>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0471" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="mint" dbAc="MI:0471" id="MINT-80017" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1579228" refType="identity" refTypeAc="MI:0356"/>
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        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
      </xref>
      <attributeList>
        <attribute name="url" nameAc="MI:0614">http://mint.bio.uniroma2.it/mint</attribute>
        <attribute name="url" nameAc="MI:0614">url&amp;gt;http://mint.bio.uniroma2.it/mint/</attribute>
        <attribute name="url" nameAc="MI:0614">https://mint.bio.uniroma2.it</attribute>
        <attribute name="id-validation-regexp" nameAc="MI:0628">MINT-[0-9]+</attribute>
        <attribute name="search-url" nameAc="MI:0615">https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac}</attribute>
        <attribute name="definition">The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules.</attribute>
        <attribute name="url" nameAc="MI:0614">http://mint.bio.uniroma2.it</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Loss of GFAT-1 feedback regulation activates the hexosamine pathway that modulates protein homeostasis.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="32019926" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-26565996" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="imex" dbAc="MI:0670" id="IM-28579" refType="imex-primary" refTypeAc="MI:0662"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Loss of GFAT-1 feedback regulation activates the hexosamine pathway that modulates protein homeostasis.</attribute>
            <attribute name="journal" nameAc="MI:0885">Nature communications</attribute>
            <attribute name="publication year" nameAc="MI:0886">2020</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Ruegenberg S., Horn M., Pichlo C., Allmeroth K., Baumann U., Denzel MS.</attribute>
            <attribute name="contact-email" nameAc="MI:0634">ulrich.baumann@uni-koeln.de;martin.denzel@age.mpg.de</attribute>
            <attribute name="author-announcement">29-Mar-2021: Contacted by IntAct-Help.</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959">imex curation</attribute>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="imex" dbAc="MI:0670" id="IM-28579" refType="imex-primary" refTypeAc="MI:0662"/>
          <secondaryRef db="pubmed" dbAc="MI:0446" id="32019926" refType="primary-reference" refTypeAc="MI:0358"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="-1">
            <names>
              <shortLabel>in vitro</shortLabel>
              <fullName>In vitro</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>x-ray diffraction</shortLabel>
            <fullName>x-ray crystallography</fullName>
            <alias type="go synonym" typeAc="MI:0303">X-ray</alias>
            <alias type="synonym" typeAc="MI:1041">X-ray</alias>
            <alias type="synonym" typeAc="MI:1041">x-ray diffraction</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0114" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1272" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
            <alias type="synonym" typeAc="MI:1041">predetermined</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Ruegenberg S., Horn M., Pichlo C., Allmeroth K., Baumann U., Denzel MS.</attribute>
          <attribute name="contact-email" nameAc="MI:0634">ulrich.baumann@uni-koeln.de;martin.denzel@age.mpg.de</attribute>
          <attribute name="accepted">Accepted 2020-DEC-23 AT 10:51 GMT AT 10:51 GMT by LUANA</attribute>
          <attribute name="journal" nameAc="MI:0885">Nature communications</attribute>
          <attribute name="publication year" nameAc="MI:0886">2020</attribute>
          <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
          <attribute name="imex curation" nameAc="MI:0959">imex curation</attribute>
          <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>q062102</shortLabel>
          <fullName>Isoform 2 of Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1</fullName>
          <alias type="gene name" typeAc="MI:0301">GFPT1</alias>
          <alias type="gene name synonym" typeAc="MI:0302">GFAT</alias>
          <alias type="gene name synonym" typeAc="MI:0302">GFPT</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="Q06210-2" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-21684272" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q06210-2" refType="isoform-parent" refTypeAc="MI:0243"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="9606">
          <names>
            <shortLabel>human</shortLabel>
            <fullName>Homo sapiens</fullName>
            <alias type="synonym" typeAc="MI:1041">Human</alias>
          </names>
        </organism>
        <sequence>MCGIFAYLNYHVPRTRREILETLIKGLQRLEYRGYDSAGVGFDGGNDKDWEANACKIQLIKKKGKVKALDEEVHKQQDMDLDIEFDVHLGIAHTRWATHGEPSPVNSHPQRSDKNNEFIVIHNGIITNYKDLKKFLESKGYDFESETDTETIAKLVKYMYDNRESQDTSFTTLVERVIQQLEGAFALVFKSVHFPGQAVGTRRGSPLLIGVRSEHKLSTDHIPILYRTGKDKKGSCNLSRVDSTTCLFPVEEKAVEYYFASDASAVIEHTNRVIFLEDDDVAAVVDGRLSIHRIKRTAGDHPGRAVQTLQMELQQIMKGNFSSFMQKEIFEQPESVVNTMRGRVNFDDYTVNLGGLKDHIKEIQRCRRLILIACGTSYHAGVATRQVLEELTELPVMVELASDFLDRNTPVFRDDVCFFLSQSGETADTLMGLRYCKERGALTVGITNTVGSSISRETDCGVHINAGPEIGVASTKAYTSQFVSLVMFALMMCDDRISMQERRKEIMLGLKRLPDLIKEVLSMDDEIQKLATELYHQKSVLIMGRGYHYATCLEGALKIKEITYMHSEGILAGELKHGPLALVDKLMPVIMIIMRDHTYAKCQNALQQVVARQGRPVVICDKEDTETIKNTKRTIKVPHSVDCLQGILSVIPLQLLAFHLAVLRGYDVDFPRNLAKSVTVE</sequence>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="3" imexId="IM-28579-1">
        <names>
          <shortLabel>1-174</shortLabel>
        </names>
        <xref>
          <primaryRef db="intact" dbAc="MI:0469" id="EBI-26566021" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="pdbe" dbAc="MI:0472" id="6r4i"/>
          <secondaryRef db="pdbe" dbAc="MI:0472" id="6r4h"/>
          <secondaryRef db="pdbe" dbAc="MI:0472" id="6svm"/>
          <secondaryRef db="pdbe" dbAc="MI:0472" id="6svq"/>
          <secondaryRef db="pdbj" dbAc="MI:0806" id="6r4j"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-28579-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="4">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <experimentalPreparationList>
              <experimentalPreparation>
                <names>
                  <shortLabel>purified</shortLabel>
                  <fullName>purified</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0350" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537811" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalPreparation>
            </experimentalPreparationList>
            <featureList>
              <feature id="5">
                <names>
                  <shortLabel>his tag</shortLabel>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-26566024" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>his tag</shortLabel>
                    <fullName>his tag</fullName>
                    <alias type="go synonym" typeAc="MI:0303">Hexa-His-tag</alias>
                    <alias type="go synonym" typeAc="MI:0303">6-His-tag</alias>
                    <alias type="go synonym" typeAc="MI:0303">Histidine-tag</alias>
                    <alias type="synonym" typeAc="MI:1041">Hexa-His-tag</alias>
                    <alias type="synonym" typeAc="MI:1041">Histidine-tag</alias>
                    <alias type="synonym" typeAc="MI:1041">6-His-tag</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0521" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-456516" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <begin position="299"/>
                    <endStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                    <end position="300"/>
                  </featureRange>
                </featureRangeList>
              </feature>
              <feature id="6">
                <names>
                  <shortLabel>Q06210-2:p.Gly461Glu</shortLabel>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-26566042" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>mutation with no effect</shortLabel>
                    <fullName>mutation with no effect</fullName>
                    <alias type="synonym" typeAc="MI:1041">mutation with no effect</alias>
                    <alias type="synonym" typeAc="MI:1041">mutation not affecting interaction</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:2226" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-11688027" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14577292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <begin position="461"/>
                    <endStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                    <end position="461"/>
                    <resultingSequence>
                      <originalSequence>G</originalSequence>
                      <newSequence>E</newSequence>
                    </resultingSequence>
                  </featureRange>
                </featureRangeList>
                <attributeList>
                  <attribute name="remark-internal">Thu Apr 22 13:36:06 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'Q06210:p.Gly461Glu'</attribute>
                </attributeList>
              </feature>
              <feature id="7">
                <names>
                  <shortLabel>Q06210-2:p.Gly451Glu</shortLabel>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-26566044" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>mutation with no effect</shortLabel>
                    <fullName>mutation with no effect</fullName>
                    <alias type="synonym" typeAc="MI:1041">mutation with no effect</alias>
                    <alias type="synonym" typeAc="MI:1041">mutation not affecting interaction</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:2226" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-11688027" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14577292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <begin position="451"/>
                    <endStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                    <end position="451"/>
                    <resultingSequence>
                      <originalSequence>G</originalSequence>
                      <newSequence>E</newSequence>
                    </resultingSequence>
                  </featureRange>
                </featureRangeList>
                <attributeList>
                  <attribute name="remark-internal">Thu Apr 22 10:24:13 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'Q06210:p.Gly451Glu'</attribute>
                </attributeList>
              </feature>
            </featureList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="7108">
                <names>
                  <shortLabel>spofr-sf_21</shortLabel>
                  <fullName>Spodoptera frugiperda insect cells</fullName>
                </names>
                <cellType>
                  <names>
                    <shortLabel>sf_21</shortLabel>
                    <fullName>Insect cells</fullName>
                  </names>
                  <xref>
                    <primaryRef db="cabri" dbAc="MI:0246" id="ACC 119" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="IA:0072" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="mint" dbAc="MI:0471" id="MINT-7501077" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-307869" refType="identity" refTypeAc="MI:0356"/>
                  </xref>
                  <attributeList>
                    <attribute name="comment" nameAc="MI:0612">derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae.</attribute>
                    <attribute name="comment" nameAc="MI:0612">full name: IPLB-SF-21-AE</attribute>
                    <attribute name="comment" nameAc="MI:0612">cells are susceptible to Baculovirus infection.</attribute>
                  </attributeList>
                </cellType>
              </hostOrganism>
            </hostOrganismList>
            <stoichiometry value="2"/>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>direct interaction</shortLabel>
            <fullName>direct interaction</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0407" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608833" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">table2 fig.1b suppl fig.2</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>