DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Coupled structural transitions enable highly cooperative regulation of human CTPS2 filaments. Coupled structural transitions enable highly cooperative regulation of human CTPS2 filaments. Nature structural & molecular biology 2019 imex curation Lynch EM., Kollman JM. jkoll@uw.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human Homo sapiens Human 3d-em 3D electron microscopy 3D-EM electron tomog experimental particp experimental participant identification experimental particp Lynch EM., Kollman JM. jkoll@uw.edu Accepted 2020-MAR-16 AT 10:45 GMT AT 10:45 GMT by PPORRAS Nature structural & molecular biology 2019 Only protein-protein interactions imex curation imex curation Coupled structural transitions enable highly cooperative regulation of human CTPS2 filaments. Coupled structural transitions enable highly cooperative regulation of human CTPS2 filaments. Nature structural & molecular biology 2019 imex curation Lynch EM., Kollman JM. jkoll@uw.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human Homo sapiens Human crosslink cross-linking study crosslink weight by staining molecular weight estimation by staining weight by staining Lynch EM., Kollman JM. jkoll@uw.edu Accepted 2020-MAR-16 AT 10:45 GMT AT 10:45 GMT by PPORRAS Nature structural & molecular biology 2019 Only protein-protein interactions imex curation imex curation pyrg2_human CTP synthase 2 UTP--ammonia ligase 2 CTP synthetase 2 CTPS2 protein protein human Homo sapiens Human MKYILVTGGVISGIGKGIIASSIGTILKSCGLRVTAIKIDPYINIDAGTFSPYEHGEVFVLNDGGEVDLDLGNYERFLDINLYKDNNITTGKIYQHVINKERRGDYLGKTVQVVPHITDAVQEWVMNQAKVPVDGNKEEPQICVIELGGTIGDIEGMPFVEAFRQFQFKAKRENFCNIHVSLVPQLSATGEQKTKPTQNSVRALRGLGLSPDLIVCRSSTPIEMAVKEKISMFCHVNPEQVICIHDVSSTYRVPVLLEEQSIVKYFKERLHLPIGDSASNLLFKWRNMADRYERLQKICSIALVGKYTKLRDCYASVFKALEHSALAINHKLNLMYIDSIDLEKITETEDPVKFHEAWQKLCKADGILVPGGFGIRGTLGKLQAISWARTKKIPFLGVCLGMQLAVIEFARNCLNLKDADSTEFRPNAPVPLVIDMPEHNPGNLGGTMRLGIRRTVFKTENSILRKLYGDVPFIEERHRHRFEVNPNLIKQFEQNDLSFVGQDVDGDRMEIIELANHPYFVGVQFHPEFSSRPMKPSPPYLGLLLAATGNLNAYLQQGCKLSSSDRYSDASDDSFSEPRIAELEIS AC1CF2E67D89741B ctps2-1 1 3 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified Q9NRF8:p.His355Ala H355A mutation mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain H A Tue Jun 30 16:34:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His355Ala' Q9NRF8:p.Val352Cys V352C mutation mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain V C Tue Jun 30 19:47:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val352Cys' yeast Saccharomyces cerevisiae Baker's yeast direct interaction direct interaction Fig 1, Fig 4, Extended Data Fig 1,2,3a-d, Table 1 ctps2-4 2 3 weight by staining molecular weight estimation by staining weight by staining predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified Q9NRF8:p.Val352Cys V352C mutation mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain V C Tue Jun 30 19:53:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val352Cys' yeast Saccharomyces cerevisiae Baker's yeast 3 weight by staining molecular weight estimation by staining weight by staining predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified Q9NRF8:p.Val352Cys V352C mutation mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain V C Tue Jun 30 18:07:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val352Cys' yeast Saccharomyces cerevisiae Baker's yeast direct interaction direct interaction Extended Data Fig 8