MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it ER-localized Hrd1 ubiquitinates and inactivates Usp15 to promote TLR4-induced inflammation during bacterial infection. ER-localized Hrd1 ubiquitinates and inactivates Usp15 to promote TLR4-induced inflammation during bacterial infection. Nature microbiology 2019 imex curation Lu Y., Qiu Y., Chen P., Chang H., Guo L., Zhang F., Ma L., Zhang C., Zheng X., Xiao J., Zhong R., Han L., Xu X., Zhang Y., Li D., Zhong G., Boyton R., Huang Y., He Y., Hu R., Wei B., Wei B., Wang H. hongyanwang@sibcb.ac.cn 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lu Y., Qiu Y., Chen P., Chang H., Guo L., Zhang F., Ma L., Zhang C., Zheng X., Xiao J., Zhong R., Han L., Xu X., Zhang Y., Li D., Zhong G., Boyton R., Huang Y., He Y., Hu R., Wei B., Wei B., Wang H. hongyanwang@sibcb.ac.cn Accepted 2020-JUL-17 AT 11:39 BST AT 11:39 BST by LUANA Nature microbiology 2019 Only protein-protein interactions imex curation imex curation ER-localized Hrd1 ubiquitinates and inactivates Usp15 to promote TLR4-induced inflammation during bacterial infection. ER-localized Hrd1 ubiquitinates and inactivates Usp15 to promote TLR4-induced inflammation during bacterial infection. Nature microbiology 2019 imex curation Lu Y., Qiu Y., Chen P., Chang H., Guo L., Zhang F., Ma L., Zhang C., Zheng X., Xiao J., Zhong R., Han L., Xu X., Zhang Y., Li D., Zhong G., Boyton R., Huang Y., He Y., Hu R., Wei B., Wei B., Wang H. hongyanwang@sibcb.ac.cn 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Lu Y., Qiu Y., Chen P., Chang H., Guo L., Zhang F., Ma L., Zhang C., Zheng X., Xiao J., Zhong R., Han L., Xu X., Zhang Y., Li D., Zhong G., Boyton R., Huang Y., He Y., Hu R., Wei B., Wei B., Wang H. hongyanwang@sibcb.ac.cn Accepted 2020-JUL-17 AT 11:39 BST AT 11:39 BST by LUANA Nature microbiology 2019 Only protein-protein interactions imex curation imex curation ER-localized Hrd1 ubiquitinates and inactivates Usp15 to promote TLR4-induced inflammation during bacterial infection. ER-localized Hrd1 ubiquitinates and inactivates Usp15 to promote TLR4-induced inflammation during bacterial infection. Nature microbiology 2019 imex curation Lu Y., Qiu Y., Chen P., Chang H., Guo L., Zhang F., Ma L., Zhang C., Zheng X., Xiao J., Zhong R., Han L., Xu X., Zhang Y., Li D., Zhong G., Boyton R., Huang Y., He Y., Hu R., Wei B., Wei B., Wang H. hongyanwang@sibcb.ac.cn 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Lu Y., Qiu Y., Chen P., Chang H., Guo L., Zhang F., Ma L., Zhang C., Zheng X., Xiao J., Zhong R., Han L., Xu X., Zhang Y., Li D., Zhong G., Boyton R., Huang Y., He Y., Hu R., Wei B., Wei B., Wang H. hongyanwang@sibcb.ac.cn Accepted 2020-JUL-17 AT 11:39 BST AT 11:39 BST by LUANA Nature microbiology 2019 Only protein-protein interactions imex curation imex curation ER-localized Hrd1 ubiquitinates and inactivates Usp15 to promote TLR4-induced inflammation during bacterial infection. ER-localized Hrd1 ubiquitinates and inactivates Usp15 to promote TLR4-induced inflammation during bacterial infection. Nature microbiology 2019 imex curation Lu Y., Qiu Y., Chen P., Chang H., Guo L., Zhang F., Ma L., Zhang C., Zheng X., Xiao J., Zhong R., Han L., Xu X., Zhang Y., Li D., Zhong G., Boyton R., Huang Y., He Y., Hu R., Wei B., Wei B., Wang H. hongyanwang@sibcb.ac.cn 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lu Y., Qiu Y., Chen P., Chang H., Guo L., Zhang F., Ma L., Zhang C., Zheng X., Xiao J., Zhong R., Han L., Xu X., Zhang Y., Li D., Zhong G., Boyton R., Huang Y., He Y., Hu R., Wei B., Wei B., Wang H. hongyanwang@sibcb.ac.cn Accepted 2020-JUL-17 AT 11:39 BST AT 11:39 BST by LUANA Nature microbiology 2019 Only protein-protein interactions imex curation imex curation ER-localized Hrd1 ubiquitinates and inactivates Usp15 to promote TLR4-induced inflammation during bacterial infection. ER-localized Hrd1 ubiquitinates and inactivates Usp15 to promote TLR4-induced inflammation during bacterial infection. Nature microbiology 2019 imex curation Lu Y., Qiu Y., Chen P., Chang H., Guo L., Zhang F., Ma L., Zhang C., Zheng X., Xiao J., Zhong R., Han L., Xu X., Zhang Y., Li D., Zhong G., Boyton R., Huang Y., He Y., Hu R., Wei B., Wei B., Wang H. hongyanwang@sibcb.ac.cn 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. confocal microscopy confocal microscopy immunostaining immunostaining Lu Y., Qiu Y., Chen P., Chang H., Guo L., Zhang F., Ma L., Zhang C., Zheng X., Xiao J., Zhong R., Han L., Xu X., Zhang Y., Li D., Zhong G., Boyton R., Huang Y., He Y., Hu R., Wei B., Wei B., Wang H. hongyanwang@sibcb.ac.cn Accepted 2020-JUL-17 AT 11:39 BST AT 11:39 BST by LUANA Nature microbiology 2019 Only protein-protein interactions imex curation imex curation ER-localized Hrd1 ubiquitinates and inactivates Usp15 to promote TLR4-induced inflammation during bacterial infection. ER-localized Hrd1 ubiquitinates and inactivates Usp15 to promote TLR4-induced inflammation during bacterial infection. Nature microbiology 2019 imex curation Lu Y., Qiu Y., Chen P., Chang H., Guo L., Zhang F., Ma L., Zhang C., Zheng X., Xiao J., Zhong R., Han L., Xu X., Zhang Y., Li D., Zhong G., Boyton R., Huang Y., He Y., Hu R., Wei B., Wei B., Wang H. hongyanwang@sibcb.ac.cn 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Lu Y., Qiu Y., Chen P., Chang H., Guo L., Zhang F., Ma L., Zhang C., Zheng X., Xiao J., Zhong R., Han L., Xu X., Zhang Y., Li D., Zhong G., Boyton R., Huang Y., He Y., Hu R., Wei B., Wei B., Wang H. hongyanwang@sibcb.ac.cn Accepted 2020-JUL-17 AT 11:39 BST AT 11:39 BST by LUANA Nature microbiology 2019 Only protein-protein interactions imex curation imex curation ER-localized Hrd1 ubiquitinates and inactivates Usp15 to promote TLR4-induced inflammation during bacterial infection. ER-localized Hrd1 ubiquitinates and inactivates Usp15 to promote TLR4-induced inflammation during bacterial infection. Nature microbiology 2019 imex curation Lu Y., Qiu Y., Chen P., Chang H., Guo L., Zhang F., Ma L., Zhang C., Zheng X., Xiao J., Zhong R., Han L., Xu X., Zhang Y., Li D., Zhong G., Boyton R., Huang Y., He Y., Hu R., Wei B., Wei B., Wang H. hongyanwang@sibcb.ac.cn 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-thp-1 Homo sapiens acute monocytic leukemia cells thp-1 Human acute monocytic leukemia cell line anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Lu Y., Qiu Y., Chen P., Chang H., Guo L., Zhang F., Ma L., Zhang C., Zheng X., Xiao J., Zhong R., Han L., Xu X., Zhang Y., Li D., Zhong G., Boyton R., Huang Y., He Y., Hu R., Wei B., Wei B., Wang H. hongyanwang@sibcb.ac.cn Accepted 2020-JUL-17 AT 11:39 BST AT 11:39 BST by LUANA Nature microbiology 2019 Only protein-protein interactions imex curation imex curation ER-localized Hrd1 ubiquitinates and inactivates Usp15 to promote TLR4-induced inflammation during bacterial infection. ER-localized Hrd1 ubiquitinates and inactivates Usp15 to promote TLR4-induced inflammation during bacterial infection. Nature microbiology 2019 imex curation Lu Y., Qiu Y., Chen P., Chang H., Guo L., Zhang F., Ma L., Zhang C., Zheng X., Xiao J., Zhong R., Han L., Xu X., Zhang Y., Li D., Zhong G., Boyton R., Huang Y., He Y., Hu R., Wei B., Wei B., Wang H. hongyanwang@sibcb.ac.cn 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-thp-1 Homo sapiens acute monocytic leukemia cells thp-1 Human acute monocytic leukemia cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lu Y., Qiu Y., Chen P., Chang H., Guo L., Zhang F., Ma L., Zhang C., Zheng X., Xiao J., Zhong R., Han L., Xu X., Zhang Y., Li D., Zhong G., Boyton R., Huang Y., He Y., Hu R., Wei B., Wei B., Wang H. hongyanwang@sibcb.ac.cn Accepted 2020-JUL-17 AT 11:39 BST AT 11:39 BST by LUANA Nature microbiology 2019 Only protein-protein interactions imex curation imex curation wdr1_human WD repeat-containing protein 1 WDR1 Actin-interacting protein 1 NORI-1 protein protein human Homo sapiens Human MPYEIKKVFASLPQVERGVSKIIGGDPKGNNFLYTNGKCVILRNIDNPALADIYTEHAHQVVVAKYAPSGFYIASGDVSGKLRIWDTTQKEHLLKYEYQPFAGKIKDIAWTEDSKRIAVVGEGREKFGAVFLWDSGSSVGEITGHNKVINSVDIKQSRPYRLATGSDDNCAAFFEGPPFKFKFTIGDHSRFVNCVRFSPDGNRFATASADGQIYIYDGKTGEKVCALGGSKAHDGGIYAISWSPDSTHLLSASGDKTSKIWDVSVNSVVSTFPMGSTVLDQQLGCLWQKDHLLSVSLSGYINYLDRNNPSKPLHVIKGHSKSIQCLTVHKNGGKSYIYSGSHDGHINYWDSETGENDSFAGKGHTNQVSRMTVDESGQLISCSMDDTVRYTSLMLRDYSGQGVVKLDVQPKCVAVGPGGYAVVVCIGQIVLLKDQRKCFSIDNPGYEPEVVAVHPGGDTVAIGGVDGNVRLYSILGTTLKDEGKLLEAKGPVTDVAYSHDGAFLAVCDASKVVTVFSVADGYSENNVFYGHHAKIVCLAWSPDNEHFASGGMDMMVYVWTLSDPETRVKIQDAHRLHHVSSLAWLDEHTLVTTSHDASVKEWTITY DIP protein O75083 original sequence version: null 8C08C31D5CD097F1 syvn1_human E3 ubiquitin-protein ligase synoviolin Synovial apoptosis inhibitor 1 SYVN1 HRD1 KIAA1810 RING-type E3 ubiquitin transferase synoviolin protein protein human Homo sapiens Human MFRTAVMMAASLALTGAVVAHAYYLKHQFYPTVVYLTKSSPSMAVLYIQAFVLVFLLGKVMGKVFFGQLRAAEMEHLLERSWYAVTETCLAFTVFRDDFSPRFVALFTLLLFLKCFHWLAEDRVDFMERSPNISWLFHCRIVSLMFLLGILDFLFVSHAYHSILTRGASVQLVFGFEYAILMTMVLTIFIKYVLHSVDLQSENPWDNKAVYMLYTELFTGFIKVLLYMAFMTIMIKVHTFPLFAIRPMYLAMRQFKKAVTDAIMSRRAIRNMNTLYPDATPEELQAMDNVCIICREEMVTGAKRLPCNHIFHTSCLRSWFQRQQTCPTCRMDVLRASLPAQSPPPPEPADQGPPPAPHPPPLLPQPPNFPQGLLPPFPPGMFPLWPPMGPFPPVPPPPSSGEAVAPPSTSAAALSRPSGAATTTAAGTSATAASATASGPGSGSAPEAGPAPGFPFPPPWMGMPLPPPFAFPPMPVPPAGFAGLTPEELRALEGHERQHLEARLQSLRNIHTLLDAAMLQINQYLTVLASLGPPRPATSVNSTEETATTVVAAASSTSIPSSEATTPTPGASPPAPEMERPPAPESVGTEEMPEDGEPDAAELRRRRLQKLESPVAH DIP protein Q86TM6 original sequence version: 128 B8A6BACBAF9673C1 ubp15_human Ubiquitin carboxyl-terminal hydrolase 15 Unph4 USP15 KIAA0529 Ubiquitin-specific-processing protease 15 Deubiquitinating enzyme 15 Unph-2 Ubiquitin thioesterase 15 protein protein human Homo sapiens Human MAEGGAADLDTQRSDIATLLKTSLRKGDTWYLVDSRWFKQWKKYVGFDSWDKYQMGDQNVYPGPIDNSGLLKDGDAQSLKEHLIDELDYILLPTEGWNKLVSWYTLMEGQEPIARKVVEQGMFVKHCKVEVYLTELKLCENGNMNNVVTRRFSKADTIDTIEKEIRKIFSIPDEKETRLWNKYMSNTFEPLNKPDSTIQDAGLYQGQVLVIEQKNEDGTWPRGPSTPKSPGASNFSTLPKISPSSLSNNYNNMNNRNVKNSNYCLPSYTAYKNYDYSEPGRNNEQPGLCGLSNLGNTCFMNSAIQCLSNTPPLTEYFLNDKYQEELNFDNPLGMRGEIAKSYAELIKQMWSGKFSYVTPRAFKTQVGRFAPQFSGYQQQDCQELLAFLLDGLHEDLNRIRKKPYIQLKDADGRPDKVVAEEAWENHLKRNDSIIVDIFHGLFKSTLVCPECAKISVTFDPFCYLTLPLPMKKERTLEVYLVRMDPLTKPMQYKVVVPKIGNILDLCTALSALSGIPADKMIVTDIYNHRFHRIFAMDENLSSIMERDDIYVFEININRTEDTEHVIIPVCLREKFRHSSYTHHTGSSLFGQPFLMAVPRNNTEDKLYNLLLLRMCRYVKISTETEETEGSLHCCKDQNINGNGPNGIHEEGSPSEMETDEPDDESSQDQELPSENENSQSEDSVGGDNDSENGLCTEDTCKGQLTGHKKRLFTFQFNNLGNTDINYIKDDTRHIRFDDRQLRLDERSFLALDWDPDLKKRYFDENAAEDFEKHESVEYKPPKKPFVKLKDCIELFTTKEKLGAEDPWYCPNCKEHQQATKKLDLWSLPPVLVVHLKRFSYSRYMRDKLDTLVDFPINDLDMSEFLINPNAGPCRYNLIAVSNHYGGMGGGHYTAFAKNKDDGKWYYFDDSSVSTASEDQIVSKAAYVLFYQRQDTFSGTGFFPLDRETKGASAATGIPLESDEDSNDNDNDIENENCMHTN DIP protein Q9Y4E8 original sequence version: 128 E81FEB9DE57F7089 ubp15_mouse Ubiquitin carboxyl-terminal hydrolase 15 Usp15 Kiaa0529 Deubiquitinating enzyme 15 Ubiquitin thioesterase 15 Ubiquitin-specific-processing protease 15 protein protein mouse Mus musculus Mouse MAEGGAADLDTQRSDIATLLKTSLRKGDTWYLVDSRWFKQWKKYVGFDSWDKYQMGDQNVYPGPIDNSGLLKDGDAQSLKEHLIDELDYILLPTEGWNKLVSWYTLMEGQEPIARKVVEQGMFVKHCKVEVYLTELKLCENGNMNNVVTRRFSKADTIDTIEKEIRKIFNIPDEKEARLWNKYMSNTFEPLNKPDSTIQDAGLYQGQVLVIEQKNEDGTWPRGPSTPKSPGASNFSTLPKISPSSLSNNYNNINNRNVKNSNYCLPSYTAYKNYDYSEPGRNNEQPGLCGLSNLGNTCFMNSAIQCLSNTPPLTEYFLNDKYQEELNFDNPLGMRGEIAKSYAELIKQMWSGKFSYVTPRAFKTQVGRFAPQFSGYQQQDCQELLAFLLDGLHEDLNRIRKKPYIQLKDADGRPDKVVAEEAWENHLKRNDSIIVDIFHGLFKSTLVCPECAKISVTFDPFCYLTLPLPMKKERSLEVYLVRMDPLAKPMQYKVIVPKIGNILDLCTALSALSGVPADKMIVTDIYNHRFHRIFAVDENLSSIMERDDIYVFEININRAEDTEHVVIPVCLREKFRHSSYTHHTGSSLFGQPFLMAIPRNNTEDKLYNLLLLRMCRYVKMSTETEETDGHLRCCEDQNINGNGPNGLHEEGSPSEMETDEPDDESSQDQELPSENENSQSEDSVGGDNDSENGLCTEETCKGQLTGHKKRLFTFQFNNLGNNDINYIKDDTSHIRFDDRQLRLDERSFLALDWDPDLKKRYFDENAAEDFEKHESVEYKPPKRPFVKLKDCIELFTTKEKLGAEDPWYCPNCKEHQQATKKLDLWSLPPVLVVHLKRFSYSRYMRDKLDTLVDFPISDLDMSEFLINPNAGPCRYNLIAVSNHYGGMGGGHYTAFAKNKDDGKWYYFDDSSVSTASEDQIVSKAAYVLFYQRQDTFSGTGFFPLDRETKGASAATGIPLESDEDSNDNDNDLENENCMHTN 6D5377C3FEA6E40A se1l1_human Protein sel-1 homolog 1 SEL1L TSA305 UNQ128/PRO1063 Suppressor of lin-12-like protein 1 protein protein human Homo sapiens Human MRVRIGLTLLLCAVLLSLASASSDEEGSQDESLDSKTTLTSDESVKDHTTAGRVVAGQIFLDSEESELESSIQEEEDSLKSQEGESVTEDISFLESPNPENKDYEEPKKVRKPALTAIEGTAHGEPCHFPFLFLDKEYDECTSDGREDGRLWCATTYDYKADEKWGFCETEEEAAKRRQMQEAEMMYQTGMKILNGSNKKSQKREAYRYLQKAASMNHTKALERVSYALLFGDYLPQNIQAAREMFEKLTEEGSPKGQTALGFLYASGLGVNSSQAKALVYYTFGALGGNLIAHMVLGYRYWAGIGVLQSCESALTHYRLVANHVASDISLTGGSVVQRIRLPDEVENPGMNSGMLEEDLIQYYQFLAEKGDVQAQVGLGQLHLHGGRGVEQNHQRAFDYFNLAANAGNSHAMAFLGKMYSEGSDIVPQSNETALHYFKKAADMGNPVGQSGLGMAYLYGRGVQVNYDLALKYFQKAAEQGWVDGQLQLGSMYYNGIGVKRDYKQALKYFNLASQGGHILAFYNLAQMHASGTGVMRSCHTAVELFKNVCERGRWSERLMTAYNSYKDGDYNAAVIQYLLLAEQGYEVAQSNAAFILDQREASIVGENETYPRALLHWNRAASQGYTVARIKLGDYHFYGFGTDVDYETAFIHYRLASEQQHSAQAMFNLGYMHEKGLGIKQDIHLAKRFYDMAAEASPDAQVPVFLALCKLGVVYFLQYIRETNIRDMFTQLDMDQLLGPEWDLYLMTIIALLLGTVIAYRQRQHQDMPAPRPPGPRPAPPQQEGPPEQQPPQ DIP protein Q9UBV2 original sequence version: 67 DIP protein Q9UBV2 original sequence version: 84 DIP protein Q9UBV2 original sequence version: 134 323EB03DC7485459 syvn1_mouse E3 ubiquitin-protein ligase synoviolin Synovial apoptosis inhibitor 1 Syvn1 Hrd1 Kiaa1810 RING-type E3 ubiquitin transferase synoviolin protein protein mouse Mus musculus Mouse MFRTAVMMAASLALTGAVVAHAYYLKHQFYPTVVYLTKSSPSMAVLYIQAFVLVFLLGKVMGKVFFGQLRAAEMEHLLERSWYAVTETCLAFTVFRDDFSPRFVALFTLLLFLKCFHWLAEDRVDFMERSPNISWLFHCRIVSLMFLLGILDFLFVSHAYHSILTRGASVQLVFGFEYAILMTMVLTIFIKYVLHSVDLQSENPWDNKAVYMLYTELFTGFIKVLLYMAFMTIMIKVHTFPLFAIRPMYLAMRQFKKAVTDAIMSRRAIRNMNTLYPDATPEELQAVDNVCIICREEMVTGAKRLPCNHIFHTSCLRSWFQRQQTCPTCRMDVLRASLPAQSPPPPEPADQGPPPAPHPQPLLPQPPNFPQGLLPPFPPGMFPLWPPMGPFPPVPPPPSSGEAAAPPPTSTAVSRPSGAATTTAAGTSTSAPAPGSVPGPEAGPAPGFPFPPPWMGMPLPPPFAFPPMPVPPAGFAGLTPEELRALEGHERQHLEARLQSLRNIHTLLDAAMLQINQYLTVLASLGPPRPATSVNPTEETASTVVSAAPSTSAPSSEAPTPSPGASPPIPEAEKPPAPESVGIVEELPEDGEPDAAELRRRRLQKLESPVAH 8F7620E5016DF1F2 1-143 1 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 10 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association fig. 4a 1-144 2 11 unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding region transmembrane domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction fig. 4b 1-145 8 11 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association fig. 4c LPS treatment 1-146 3 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed ha tag ha ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9Y4E8:p.Lys21Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K R Thu Sep 17 11:28:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.lys21arg' Q9Y4E8:p.Lys154Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K R Wed Sep 16 10:53:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.lys154arg' Q9Y4E8:p.Lys228Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K R Wed Sep 16 10:22:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.lys228arg' 10 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association fig. 4e; suppl. fig.4f, suppl. fig. 5b 1-147 7 13 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q86TM6:p.Cys329Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain C S Fri Sep 18 22:02:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.cys329ser' physical association physical association suppl.fig.3c 1-148 4 12 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 14 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association suppl. fig.4b usp15-syvn1-1 8 13 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 11 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association suppl. fig. 4c LPS treatment 1-149 5 11 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association suppl.fig. 4d Immunofluorescence assays also showed that Usp15 colocalised with the ER marker Calnexin, and this colocalisation was enhanced by LPS treatment (Figure S4e, Pearson’s correlation value was increased from 0.58 to 0.68). usp15-syvn1-2 3 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association suppl. fig. 4g 1-152 3 9 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed ha tag ha ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association suppl. fig 4a 1-154 6 11 unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep 10 unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep colocalization colocalization fig. 4d LPS treatment