MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it The APMAP interactome reveals new modulators of APP processing and beta-amyloid production that are altered in Alzheimer's disease. The APMAP interactome reveals new modulators of APP processing and beta-amyloid production that are altered in Alzheimer's disease. Acta neuropathologica communications 2019 imex curation Gerber H., Mosser S., Boury-Jamot B., Stumpe M., Piersigilli A., Goepfert C., Dengjel J., Albrecht U., Magara F., Fraering PC. Alzheimers - Interactions investigated in the context of Alzheimers disease fraeringpatrick@hotmail.com 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. bn-page blue native page bn-page sequence tag sequence tag identification sequence tag Gerber H., Mosser S., Boury-Jamot B., Stumpe M., Piersigilli A., Goepfert C., Dengjel J., Albrecht U., Magara F., Fraering PC. Alzheimers - Interactions investigated in the context of Alzheimers disease fraeringpatrick@hotmail.com Accepted 2021-JAN-27 AT 16:34 GMT AT 16:34 GMT by MGT Acta neuropathologica communications 2019 Only protein-protein interactions imex curation imex curation a0a061i3k8_crigr H671_7g18220 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MEGKWLLCLLLVLGTVAVQAHDGHDDDMIDIEDDLDDVIEEVEDSKSKSDSSTPPSPKVTYKAPVPTGEVYFADSFDRGSLSGWILSKAKKDDTDDEIAKYDGKWEVDEMKDTKLPGDKGLVLMSRAKHHAISAKLNKPFLFDTKPLIVQYEVNFQNGIECGGAYVKLLSKTSELNLDQFHDKTPYTIMFGPDKCGEDYKLHFIFRHKNPKTGVYEEKHAKRPDTDLKIYFTDKKTHLYTLILNPDNSFEILVDQYVVNSGNLLNDMTPAVNPSREIEDPEDQKPEDWDERPKIPDPDAVKPDDWDEDAPGKIPDEEATKPEGWLDDEPEYIPDPDADKPEDWDEDMDGEWEAPQIANPKCESAPGCGVWQRPLIDNPNYKGKWKPPMIDNPNYQGVWKPRKIPNPDFFEDLEPFKMTPFSAIGLELWSMTSDIFFDNFIISGDRRVVDDWANDGWGLKKAADGAAEPGVVGHMLEAAEERPWLWVVYILTVALPVFLVILFCCSGKKQSNAMEYKKTDAAQPDVKEDEGKEEEKNKGDEEEEEEKLEEKQKSDAEEDGGTGSQDEEDRKPTAEEDEILNRSPRNRKPRRESWDLWHLHDTVKRNLDSAASPQNGDQQNGYGDLFPGHKKTRREAPLGISVSANGLPPASPLGQPDKPPGGDALQSGGKHSLGLDPINKKCMADSSIHLNGSSNPNEPFPLSLSKELKQEPVDDLPCMIAGAGGSVSQSNLMPDLNLNEQEWKELIEELNRSVPDEDMKDLFTEDFEEKKDPEPPGSATQTPLAQDINIKTEFSPAAFEQEQLGSPQVRAGSAGQTFLGPSSAPVGTDSPSLGSSQTLFHTTGQPGADNPSPNLMPASAQAQNAQRALASVVLPSQGPGGASELSSAHQLQQIAAKQKREQMLQNPQQAASAPAPGQLSTWQQAGPSHSPLDVPYPMEKPASPPGYKQDFTNSKLLMMPSVNKSSPRPGGPYLQPSHSNLLSHQSPSNLNQSSVNNQGAVLDYGNTKPLSHYKADCGQGGPGSGQSKPALMAYLPQQLPHLSNEQNSLFLMKQKAANMPFRSLVPPGQEQNPSNVSVPAQTANVGTQPPSVSVASTHNSSPYLSSQQQAAVMKQHQLLLDQQKQREQQQLQQQQQQFLQRQHLLAEQEKQQFQRPSAAVPGMNNLGPSNSSCPRVFPQPGTLMSMGPGHAPVSSLPSSSGPQDRGVAQFTGSQSLPQSSLYGITSGLPQIVAQPPPQAASAHAHIPRQTSVGQNASAPAAYGQNSLGSAGLSQQHNKGTLPPGLTKSQVPRVSAAMGGQNASWQHQGMPNLSSQTPGNSSVSPFAAPSSFHMQQAHLKIPGQQFSQAMPSRPMAPMSSAGAAGSMLPPVSTQQRNSAPAPAPPQAAPQQGLPGLSPAGPELGAFSQSSTSQMNSRAGLHCTQAYPVRTMGQELPFAFSGQPGSSGLSSVAGHTDLIDSLLKNRTSEEWINELDDLLGSQ DB31EB6077B5186E apmap_human Adipocyte plasma membrane-associated protein Protein BSCv APMAP C20orf3 UNQ1869/PRO4305 protein protein human Homo sapiens Human MSEADGLRQRRPLRPQVVTDDDGQAPEAKDGSSFSGRVFRVTFLMLAVSLTVPLLGAMMLLESPIDPQPLSFKEPPLLLGVLHPNTKLRQAERLFENQLVGPESIAHIGDVMFTGTADGRVVKLENGEIETIARFGSGPCKTRDDEPVCGRPLGIRAGPNGTLFVADAYKGLFEVNPWKREVKLLLSSETPIEGKNMSFVNDLTVTQDGRKIYFTDSSSKWQRRDYLLLVMEGTDDGRLLEYDTVTREVKVLLDQLRFPNGVQLSPAEDFVLVAETTMARIRRVYVSGLMKGGADLFVENMPGFPDNIRPSSSGGYWVGMSTIRPNPGFSMLDFLSERPWIKRMIFKLFSQETVMKFVPRYSLVLELSDSGAFRRSLHDPDGLVATYISEVHEHDGHLYLGSFRSPFLCRLSLQAV C88E2DF2DF6093C4 g3h7x9_crigr Nicastrin I79_006475 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MVLLEGKLFNRAVMEKLKGRTSRIAGLAVTLAKPNSTSSFSPSVQCPNDGFGIYSNSYGSEFAHCKQTLWNELGNGLAYEDFSFPIFLLEDENETKVIKQCYQDHNLAQNGSAPRFPLCAMQLFSHMHAVISTATCMRRSFIQSTFSINPEIVCDPLSDYNVWSMLKPINTSGALEPDDRVVVAATRLDSRSFFWNVAPGAESAVASYVTQLAAAEALHKASDVATLPRNVMFVFFQGETFDYIGSSRMVYDMENGKFPVRLENIDSFVELGQVALRTSLDLWMHTDPMSQKNESVKNQVEDLLVTLEKSGAGVPEVVLRRQNQSQALPPSSLQRFLRARNISGVVLADHSGSFHNRYYQSIYDTAENINVTYPERQSPEEDLNFVTDTAKALANVATVLARALYELAGGTNFSDSIQADPQTVTRLLYGFLVRANNSWFQSILRQDLRSYLDDGPLQHYIAVTSPTNTTYVVQYALANLTGKVTNLTREQCQDPSKVPNESKALYDYSWVQGPLNSNKTERLPRCVRSTVRLARALSPAFELSQWGSTEYSTWAESRWKDIQARIFLVASKELEFITLTVGFGILIFSLIVTYCINAKADVLFVAPREPGAVSY D94886BBF3F5DA49 rtn4_crigr_protein Reticulon-4 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster g3hyq1_crigr I79_016192 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MEKASLIQKAKLAEQAERYEDMAAFMKGAVEKGEELSCEERNLLSVAYKNVVGGQRAAWRVLSSIEQKSNEEGSEEKGPEVKEYREKVETELRGVCDTVLGLLDSHLIKEAGEAGSRVFYLKMKGDYYRYLAEVATGDDKKRIIDSARSAYQEAMDISKKEMPPTNPIRLGLALNFSVFHYEIANSPEEAISLAKTTFDEAMADLHTLNEDSYKDSTLIMQLLRDNLTLWTADSAGEEGGEAPEEPQS D5F4B9223E4D33B7 g3h2e5_crigr Olfactory receptor I79_004355 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MSKGRENETGVSEFLLLGITNDSGQQQILFWAFLCMYLVTVAGNTLILLAISSDPRLHTPMYFFLANLSFVDICFTTNLIPRLLASHVAGTRTISYTHCLTQMYFLISFANVDTFLLAAMALDRFVAICTIITPQLCVGLAAIVWVCSGLISLLHTLLMSRLNFCSLLPEISHFYCDAYLIMKSACSDTRVNQRVFLGAVVLFVAPCILIVVSYVRITIAVFRIPSAKGRYKAFSTCSSHLSVVTLFYGTVLGIYIRPPDSFSTQDTVATIMYTVVTPMLNPFIYSLRNKDMKETMTRLLNKGLKSS BD8C596842B17E7B clus_crigr_protein Clusterin protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster similar to P10909 fetua_crigr_protein Fetuin-A protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster similar to P02765 g3hgq1_crigr I79_009791 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MPLVCSLYVHPLPLLIGDSLLRSSSRSLLEFNTTTDCQPSDSQHRTQTSITFLCGKTLGTPEFVTATDCVHYFEWRTTAACKKDIFKADKEVPCYAFDDKLKKHDLNPLIKLSGGYLVDDSDPETSLFINVCRDIDSLRDPSTQLRACPAGTAACLLKGDQAFDVGRPKEGLKLVSKDRLVLSYVKEEAEKPDFCNGHSPAVTITFVCPSERREGTIPKLTASSNCRYEVEWITEYACHRDYLESETCSLSSEQHDIAIDLSPLAQQGGSSYVSDGREYTFYINVCGNATVPLCNLKESAVCQVKKADSTQVKIAGRHQNQTLRYSDGDLTLIYSGGDECSSGFQRMSVINFECNKTAGNDGRGEPVFTGEVDCTYFFTWDTRYACVKEKEDLLCGVSDGKKRYDLSVLARHSESEKNWEAVDGDQAESEKKYFFINVCHRVLQEGKARSCPEDAAVCAVDKNGSKNLGKFVSSPTSEKGYIQLSYSDGDDCGSDKKITTNITLVCKPGDLESAPVLRTAGPDGCFYEFEWHTAAACVLSKTEGENCTVFDAQAGFSFDLSPLTKKNGAYKVGTEKYDFYINVCGPVSVDLCQPNSGACQVAKRQEEDNSTYNFRWYTSYACPEEPLECMVTDPSMMEQYDLSSLVKSEGRSGGNWYAMENSREHVTRRKYYINVCRPLNPVPGCDRYAAACQMRYENSEGSLAETVSISNLGVAKTGPVVEESGSLLLEYVNGSACTTSDGRLTTYSTRIHLVCGRGSLNTHPIFTYNWECVVSFLWNTEAACPIQTITDTDQACSIRDPNSGFVFNLSPLNRSQGYVVPGIGKNFVFNICGAMPECGTIAGNPAFGCEAGTQTELESLKPERPVGMEKSLQLSTEGFLTLTYKGSSPSDKGTAFIIRFICNDDIYPGTAKFLHQDIDSTRGIRNTFFEFETALACVPSRVDCQVTDPAGNEYDLSALSMAGKPWTAVDTSVDGKKRSFYLSVCTPLPYIPGCHGSAVGSCMVLPAEDRGLNLGVVQISPQADANGSLSILYVNGDKCGNQRFSTRIIFECAQTLGSPVFQFVKDCEYMFVWRTVEACPVVREEGDNCQVKDPRHGNFYDLKPLALSDTIITAGEYTYYFRVCGKLSSNVCHVRDGSKVVSSCQEKKGSQGFQKVAGLLTQKLTYENGLLKMNYTGGDTCHQVYQRSTTIYFYCDRSTQKPVFLRETTDCSYLFEWRTQYACPPFNVTECSTQDGAGNSIDLSSLSRYSDNWEAVTRTGATEHYLINICKSLSPRAGGGEPCPPEAAVCLLDGSKPLNLGKVRDGPQWTGGVTVLKYVDGDLCPDQIRKRSTIIRFTCSESQVSSRPLFISAVQDCEYTFSWPTPAACPVKNSTHDDCQVTNPSTGHLFDLSSLSGKAGVTVSYSEKGLVYMSICGENENCGAGVGACFGQTRISVGKASKRLSYVDQVLQLVYEEGSLCPSKSGLRYKSVISFVCRPEAGPTNRPMLISLDKQTCTLFFSWHTPLACEQVTECTVRNGSSIIDLSPLIHRTGGYEAYDESEDGTSDTTPDFYINICQPLNPMHGVPCPAGAAVCKVPVNGPPIDIGRVTGPPIFNPVANEVYLNFESSTPCLADKHMNYTSLIAFHCKRGVSMGTPKLIRTNDCDFVFEWETPIVCPDEVKTQGCSVTDEQLLYSFNLTSLSTSTFKARLPSIARGSD 79BF854978C5D310 g3h902_crigr I79_006863 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MASVCRGRVVRVPSGSLVRVVGTELVIPCNVSDYDGPIEQNFDWSFSSSGSSFLELASTWEPGFPAQLYRERLQRGDILLRRTANDAVELHIKNVQPSDQGHYKCSTPSTDATVQGNYEDTVQVKVLADALVVGPSSRPPPGLSLREGEPFELRCITSTMSPLHTHLALRWELHRGPVHRSILALSHEGRFHPGPGYEQRYHSGDVRLDTAGSDAYRLSVARALSADQGSYRCVVSEWITEQGNWQEIQEKAVEVATVVIQPTALQLAVPRTVSVTEGKDLDLSCNITTDRVDDVRPEVTWYFKKTPDASLLASHMLARLDRDSLVHSSPHVALSHVDTRSYHLLVRDASKENSGYYLCLVALWAPGHNRSWHKVAEAMSAPSSVSVTWLEPEYQVYLNASKVPGFSDDPTELQCRVIDMKRVEAGVRLTVSWYYRMNRRIGDEVVASELLAVMDGDWTLRYGERSKQRAQSGEFIFSKEHTDTFSFRIQRTTEEDRGNYYCVVSAWTRQRNNSWVKSKDVFSKPVNIFWASEDSVLVVRARQPKPFFAAGNTFEMTCKVSSKNIKSPRYSVLITAEKPVGDLTSPNETKYIISLDQDSVVKLENWTDASRVDGVVLEKVQEDEFRYRMYQTQVSDAGLYRCMVTAWSPIGGSLWREAATSLSNPIEIDFQTSDDMAFDVSWFAVHSFGLDKDPVLLSSLDRKGVVTTGQRDWKSTLSLERVSVLEFLLQVHGSEDQDFGNYYCSVTPWVRSPTGSWQREAEIHSRPIFITVKMDVLNAFKYPLLIGVGLSTVIGLLSCLIGYCSSHWCCKKEVRETRRERRRLMSMEMD 846E43F576003862 mbrl_crigr_protein Membralin protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster similar to F1LPC5 argi-1_crigr_protein Arginase-1 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster spb3_crigr_protein Serpin B3 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster similar to P29508 lamp1_crigr Lysosome-associated membrane glycoprotein 1 CD107 antigen-like family member A Lysosomal membrane glycoprotein A LAMP1 LGPA protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MAAPGAPRSLLLLLLAGLAHGASALFVVKDSNGTACIMANFSASFFTIYETGHGSKNSTFELPSSAEVLNSNSSCGRENVSEPILTIAFGSGYLLTLNFTRNATRYSVQDMYFAYNLSDTQHFLNASNKGIHSVDSSTDIKADINKTYRCLSAIQVHMGNVTVTLSDATIQAYLLNSNFSKEETRCTQDGPSPTTVPPSPSPPLVPTNPTVIKYNVTGENGTCLLASMALQMNITYMKKDNMTVTRALNISPNDTASGSCSPHVVTLTVESKNSILDLKFGMNGSSSLFFLQEVRLNMTLPDANVSSLMASNQSLRALQATVGNSYKCNTEEHIFVTKEFSLNVFSVQVQAFKVESDRFGSVEECMQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKRSHAGYQTI 651002040F86BB3D ahsg-1 1 7 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 13 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 6 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 14 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 10 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 11 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 4 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 15 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 12 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed partially purified partially purified flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 2 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 5 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. association association F3D SF4 We next designed a multistep purification procedure for the high-grade purification of APMAP and APMAP protein complexes. he Flag-tagged APMAP and APMAP protein complexes were affinity purified following a previously described M2 anti-Flag antibody immuno-affinity procedure [40]. As a last purification step, the proteins eluted from the M2 resin were separated by size exclusion chromatography (SEC) and the fractions enriched in APMAP (Fig. (Fig.3b)3b) were further loaded onto a Blue Native gel (BN-PAGE), which allows the detection of APMAP protein complexes in their native conformations.