UniProt UniProt http://www.uniprot.org De novo mutations in MSL3 cause an X-linked syndrome marked by impaired histone H4 lysine 16 acetylation. De novo mutations in MSL3 cause an X-linked syndrome marked by impaired histone H4 lysine 16 acetylation. Nature genetics 2018 imex curation Basilicata MF., Bruel AL., Semplicio G., Valsecchi CIK., Aktaş T., Duffourd Y., Rumpf T., Morton J., Bache I., Szymanski WG., Gilissen C., Vanakker O., Õunap K., Mittler G., van der Burgt I., El Chehadeh S., Cho MT., Pfundt R., Tan TY., Kirchhoff M., Menten B., Vergult S., Lindstrom K., Reis A., Reis A., Johnson DS., Fryer A., McKay V., null null., Fisher RB., Thauvin-Robinet C., Francis D., Roscioli T., Pajusalu S., Radtke K., Ganesh J., Brunner HG., Wilson M., Faivre L., Kalscheuer VM., Thevenon J., Akhtar A. akhtar@ie-freiburg.mpg.de 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 flp-in Homo sapiens 293 flp-in 293 flp-in anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Basilicata MF., Bruel AL., Semplicio G., Valsecchi CIK., Aktaş T., Duffourd Y., Rumpf T., Morton J., Bache I., Szymanski WG., Gilissen C., Vanakker O., Õunap K., Mittler G., van der Burgt I., El Chehadeh S., Cho MT., Pfundt R., Tan TY., Kirchhoff M., Menten B., Vergult S., Lindstrom K., Reis A., Reis A., Johnson DS., Fryer A., McKay V., null null., Fisher RB., Thauvin-Robinet C., Francis D., Roscioli T., Pajusalu S., Radtke K., Ganesh J., Brunner HG., Wilson M., Faivre L., Kalscheuer VM., Thevenon J., Akhtar A. akhtar@ie-freiburg.mpg.de Accepted 2020-NOV-03 AT 12:19 GMT AT 12:19 GMT by KALPANAP Nature genetics 2018 Only protein-protein interactions imex curation imex curation De novo mutations in MSL3 cause an X-linked syndrome marked by impaired histone H4 lysine 16 acetylation. De novo mutations in MSL3 cause an X-linked syndrome marked by impaired histone H4 lysine 16 acetylation. Nature genetics 2018 imex curation Basilicata MF., Bruel AL., Semplicio G., Valsecchi CIK., Aktaş T., Duffourd Y., Rumpf T., Morton J., Bache I., Szymanski WG., Gilissen C., Vanakker O., Õunap K., Mittler G., van der Burgt I., El Chehadeh S., Cho MT., Pfundt R., Tan TY., Kirchhoff M., Menten B., Vergult S., Lindstrom K., Reis A., Reis A., Johnson DS., Fryer A., McKay V., null null., Fisher RB., Thauvin-Robinet C., Francis D., Roscioli T., Pajusalu S., Radtke K., Ganesh J., Brunner HG., Wilson M., Faivre L., Kalscheuer VM., Thevenon J., Akhtar A. akhtar@ie-freiburg.mpg.de 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Basilicata MF., Bruel AL., Semplicio G., Valsecchi CIK., Aktaş T., Duffourd Y., Rumpf T., Morton J., Bache I., Szymanski WG., Gilissen C., Vanakker O., Õunap K., Mittler G., van der Burgt I., El Chehadeh S., Cho MT., Pfundt R., Tan TY., Kirchhoff M., Menten B., Vergult S., Lindstrom K., Reis A., Reis A., Johnson DS., Fryer A., McKay V., null null., Fisher RB., Thauvin-Robinet C., Francis D., Roscioli T., Pajusalu S., Radtke K., Ganesh J., Brunner HG., Wilson M., Faivre L., Kalscheuer VM., Thevenon J., Akhtar A. akhtar@ie-freiburg.mpg.de Accepted 2020-NOV-03 AT 12:19 GMT AT 12:19 GMT by KALPANAP Nature genetics 2018 Only protein-protein interactions imex curation imex curation msl1_human Male-specific lethal 1 homolog MSL1 MSL1L1 Male-specific lethal 1-like 1 Male-specific lethal-1 homolog 1 protein protein human Homo sapiens Human MTMRSAVFKAAAAPAGGNPEQRLDYERAAALGGPEDEPGAAEAHFLPRHRKLKEPGPPLASSQGGSPAPSPAGCGGKGRGLLLPAGAAPGQQEESWGGSVPLPCPPPATKQAGIGGEPAAAGAGCSPRPKYQAVLPIQTGSLVAAAKEPTPWAGDKGGAASPAATASDPAGPPPLPLPGPPPLAPTATAGTLAASEGRWKSMRKSPLGGGGGSGASSQAACLKQILLLQLDLIEQQQQQLQAKEKEIEELKSERDTLLARIERMERRMQLVKKDNEKERHKLFQGYETEEREETELSEKIKLECQPELSETSQTLPPKPFSCGRSGKGHKRKSPFGSTERKTPVKKLAPEFSKVKTKTPKHSPIKEEPCGSLSETVCKRELRSQETPEKPRSSVDTPPRLSTPQKGPSTHPKEKAFSSEIEDLPYLSTTEMYLCRWHQPPPSPLPLRESSPKKEETVARCLMPSSVAGETSVLAVPSWRDHSVEPLRDPNPSDLLENLDDSVFSKRHAKLELDEKRRKRWDIQRIREQRILQRLQLRMYKKKGIQESEPEVTSFFPEPDDVESLMITPFLPVVAFGRPLPKLTPQNFELPWLDERSRCRLEIQKKQTPHRTCRK DIP protein Q68DK7 original sequence version: 75 D48D845C1C2ABF45 q8n5y2-1 Male-specific lethal 3 homolog MSL3 MSL3L1 Male-specific lethal-3 homolog 1 Male-specific lethal-3 protein-like 1 protein protein human Homo sapiens Human MSASEGMKFKFHSGEKVLCFEPDPTKARVLYDAKIVDVIVGKDEKGRKIPEYLIHFNGWNRSWDRWAAEDHVLRDTDENRRLQRKLARKAVARLRSTGRKKKRCRLPGVDSVLKGLPTEEKDENDENSLSSSSDCSENKDEEISEESDIEEKTEVKEEPELQTRREMEERTITIEIPEVLKKQLEDDCYYINRRKRLVKLPCQTNIITILESYVKHFAINAAFSANERPRHHHVMPHANMNVHYIPAEKNVDLCKEMVDGLRITFDYTLPLVLLYPYEQAQYKKVTSSKFFLPIKESATSTNRSQEELSPSPPLLNPSTPQSTESQPTTGEPATPKRRKAEPEALQSLRRSTRHSANCDRLSESSASPQPKRRQQDTSASMPKLFLHLEKKTPVHSRSSSPIPLTPSKEGSAVFAGFEGRRTNEINEVLSWKLVPDNYPPGDQPPPPSYIYGAQHLLRLFVKLPEILGKMSFSEKNLKALLKHFDLFLRFLAEYHDDFFPESAYVAACEAHYSTKNPRAIY DIP protein Q8N5Y2-1 original sequence version: 78 q68dk7-1 Male-specific lethal 1 homolog MSL1 MSL1L1 Male-specific lethal 1-like 1 Male-specific lethal-1 homolog 1 protein protein human Homo sapiens Human MTMRSAVFKAAAAPAGGNPEQRLDYERAAALGGPEDEPGAAEAHFLPRHRKLKEPGPPLASSQGGSPAPSPAGCGGKGRGLLLPAGAAPGQQEESWGGSVPLPCPPPATKQAGIGGEPAAAGAGCSPRPKYQAVLPIQTGSLVAAAKEPTPWAGDKGGAASPAATASDPAGPPPLPLPGPPPLAPTATAGTLAASEGRWKSMRKSPLGGGGGSGASSQAACLKQILLLQLDLIEQQQQQLQAKEKEIEELKSERDTLLARIERMERRMQLVKKDNEKERHKLFQGYETEEREETELSEKIKLECQPELSETSQTLPPKPFSCGRSGKGHKRKSPFGSTERKTPVKKLAPEFSKVKTKTPKHSPIKEEPCGSLSETVCKRELRSQETPEKPRSSVDTPPRLSTPQKGPSTHPKEKAFSSEIEDLPYLSTTEMYLCRWHQPPPSPLPLRESSPKKEETVARCLMPSSVAGETSVLAVPSWRDHSVEPLRDPNPSDLLENLDDSVFSKRHAKLELDEKRRKRWDIQRIREQRILQRLQLRMYKKKGIQESEPEVTSFFPEPDDVESLMITPFLPVVAFGRPLPKLTPQNFELPWLDERSRCRLEIQKKQTPHRTCRK D48D845C1C2ABF45 q9h7z6-1 Histone acetyltransferase KAT8 Lysine acetyltransferase 8 MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1 KAT8 MOF MYST1 PP7073 protein protein human Homo sapiens Human MAAQGAAAAVAAGTSGVAGEGEPGPGENAAAEGTAPSPGRVSPPTPARGEPEVTVEIGETYLCRRPDSTWHSAEVIQSRVNDQEGREEFYVHYVGFNRRLDEWVDKNRLALTKTVKDAVQKNSEKYLSELAEQPERKITRNQKRKHDEINHVQKTYAEMDPTTAALEKEHEAITKVKYVDKIHIGNYEIDAWYFSPFPEDYGKQPKLWLCEYCLKYMKYEKSYRFHLGQCQWRQPPGKEIYRKSNISVYEVDGKDHKIYCQNLCLLAKLFLDHKTLYFDVEPFVFYILTEVDRQGAHIVGYFSKEKESPDGNNVACILTLPPYQRRGYGKFLIAFSYELSKLESTVGSPEKPLSDLGKLSYRSYWSWVLLEILRDFRGTLSIKDLSQMTSITQNDIISTLQSLNMVKYWKGQHVICVTPKLVEEHLKSAQYKKPPITVDSVCLKWAPPKHKQVKLSKK 66C474BE5B90E8E3 kat8_human Histone acetyltransferase KAT8 KAT8 MOF PP7073 MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1 MYST1 Lysine acetyltransferase 8 protein protein human Homo sapiens Human MAAQGAAAAVAAGTSGVAGEGEPGPGENAAAEGTAPSPGRVSPPTPARGEPEVTVEIGETYLCRRPDSTWHSAEVIQSRVNDQEGREEFYVHYVGFNRRLDEWVDKNRLALTKTVKDAVQKNSEKYLSELAEQPERKITRNQKRKHDEINHVQKTYAEMDPTTAALEKEHEAITKVKYVDKIHIGNYEIDAWYFSPFPEDYGKQPKLWLCEYCLKYMKYEKSYRFHLGQCQWRQPPGKEIYRKSNISVYEVDGKDHKIYCQNLCLLAKLFLDHKTLYFDVEPFVFYILTEVDRQGAHIVGYFSKEKESPDGNNVACILTLPPYQRRGYGKFLIAFSYELSKLESTVGSPEKPLSDLGKLSYRSYWSWVLLEILRDFRGTLSIKDLSQMTSITQNDIISTLQSLNMVKYWKGQHVICVTPKLVEEHLKSAQYKKPPITVDSVCLKWAPPKHKQVKLSKK 66C474BE5B90E8E3 msl3-msl1-2 1 7 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association Fig.1H msl3-kat8-4 2 6 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 4 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain association association Fig.1E kat8-msl1-4 2 6 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig.1E