IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ miR-302a-5p/367-3p-HMGA2 axis regulates malignant processes during endometrial cancer development. miR-302a-5p/367-3p-HMGA2 axis regulates malignant processes during endometrial cancer development. Journal of experimental & clinical cancer research : CR 2018 imex curation Ma J., Li D., Kong FF., Yang D., Yang H., Ma XX. maxiaoxin666@aliyun.com Only protein-protein interactions imex curation human-ishikawa_cell_line human Ishikawa ERalpha-positive endometrium adenocarcinoma Human ishikawa_cell_line ERalpha-positive endometrium adenocarcinoma ECACC Catalogue No.: 99040201 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ma J., Li D., Kong FF., Yang D., Yang H., Ma XX. maxiaoxin666@aliyun.com Accepted 2020-JUN-07 AT 09:46 BST AT 09:46 BST by KALPANAP Journal of experimental & clinical cancer research : CR 2018 Only protein-protein interactions imex curation imex curation miR-302a-5p/367-3p-HMGA2 axis regulates malignant processes during endometrial cancer development. miR-302a-5p/367-3p-HMGA2 axis regulates malignant processes during endometrial cancer development. Journal of experimental & clinical cancer research : CR 2018 imex curation Ma J., Li D., Kong FF., Yang D., Yang H., Ma XX. maxiaoxin666@aliyun.com Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag luciferase tag visualisation by luciferase assay tag luciferase Ma J., Li D., Kong FF., Yang D., Yang H., Ma XX. maxiaoxin666@aliyun.com Accepted 2020-JUN-07 AT 09:46 BST AT 09:46 BST by KALPANAP Journal of experimental & clinical cancer research : CR 2018 Only protein-protein interactions imex curation imex curation miR-302a-5p/367-3p-HMGA2 axis regulates malignant processes during endometrial cancer development. miR-302a-5p/367-3p-HMGA2 axis regulates malignant processes during endometrial cancer development. Journal of experimental & clinical cancer research : CR 2018 imex curation Ma J., Li D., Kong FF., Yang D., Yang H., Ma XX. maxiaoxin666@aliyun.com Only protein-protein interactions imex curation human-hec1a Homo sapiens hec1a human endometrial carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ma J., Li D., Kong FF., Yang D., Yang H., Ma XX. maxiaoxin666@aliyun.com Accepted 2020-JUN-07 AT 09:46 BST AT 09:46 BST by KALPANAP Journal of experimental & clinical cancer research : CR 2018 Only protein-protein interactions imex curation imex curation mrna_hmga2 mrna high mobility group AT-hook 2 mrna messenger rna mRNA mrna human Homo sapiens Human CAGCCCGGGATCCCCGGCCTGTCCCTTTAACCCCGCCGCCGGGCGGGAGCACGTGAGCGGGGCTCCGGGTGGCACCCGGGCGCCGCCGCCGCCGAGGCAGTTGTATTTCGAACGCTGCCTCTGGCTAGCAGCCAGGCGCCTTGGCTCGGCGGTCCGCCTGGCCTCCCTCCTCCTCATACTTTTCTTCCTGCGCAACCCCCTCCCCTTTATCCGCCCACGATTAGAGGTGGGCACTCCCCCCACCACCACCCCCTCCCCAAGCGCAAGCGCGTGCACGCACACACACCACACACACTCACACTCACACACACTCACACACACTCATCCCACTTGAATCTTGGGGCAGGAACTCAGAAAACTTCCAGCCCGGGCAGCGCGCGCTTGGTGCAAGACTCAGGAGCTAGCAGCCCGTCCCCCTCCGACTCTCCGGTGCCGCCGCTGCCTGCTCCCGCCACCCTAGGAGGCGCGGTGCCACCCACTACTCTGTCCTCTGCCTGTGCTCCGTGCCCGACCCTATCCCGGCGGAGTCTCCCCATCCTCCTTTGCTTTCCGACTGCCCAAGGCACTTTCAATCTCAATCTCTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCGCAGGGTGGGGGGAAGAGGAGGAGGAATTCTTTCCCCGCCTAACATTTCAAGGGACACAATTCACTCCAAGTCTCTTCCCTTTCCAAGCCGCTTCCGAAGTGCTCCCGGTGCCCGCAACTCCTGATCCCAACCCGCGAGAGGAGCCTCTGCGACCTCAAAGCCTCTCTTCCTTCTCCCTCGCTTCCCTCCTCCTCTTGCTACCTCCACCTCCACCGCCACCTCCACCTCCGGCACCCACCCACCGCCGCCGCCGCCACCGGCAGCGCCTCCTCCTCTCCTCCTCCTCCTCCCCTCTTCTCTTTTTGGCAGCCGCTGGACGTCCGGTGTTGATGGTGGCAGCGGCGGCAGCCTAAGCAACAGCAGCCCTCGCAGCCCGCCAGCTCGCGCTCGCCCCGCCGGCGTCCCCAGCCCTATCACCTCATCTCCCGAAAGGTGCTGGGCAGCTCCGGGGCGGTCGAGGCGAAGCGGCTGCAGCGGCGGTAGCGGCGGCGGGAGGCAGGATGAGCGCACGCGGTGAGGGCGCGGGGCAGCCGTCCACTTCAGCCCAGGGACAACCTGCCGCCCCAGCGCCTCAGAAGAGAGGACGCGGCCGCCCCAGGAAGCAGCAGCAAGAACCAACCGGTGAGCCCTCTCCTAAGAGACCCAGGGGAAGACCCAAAGGCAGCAAAAACAAGAGTCCCTCTAAAGCAGCTCAAAAGAAAGCAGAAGCCACTGGAGAAAAACGGCCAAGAGGCAGACCTAGGAAATGGCCACAACAAGTTGTTCAGAAGAAGCCTGCTCAGGAGGAAACTGAAGAGACATCCTCACAAGAGTCTGCCGAAGAGGACTAGGGGGCGCCAACGTTCGATTTCTACCTCAGCAGCAGTTGGATCTTTTGAAGGGAGAAGACACTGCAGTGACCACTTATTCTGTATTGCCATGGTCTTTCCACTTTCATCTGGGGTGGGGTGGGGTGGGGTGGGGGAGGGGGGGGTGGGGTGGGGAGAAATCACATAACCTTAAAAAGGACTATATTAATCACCTTCTTTGTAATCCCTTCACAGTCCCAGGTTTAGTGAAAAACTGCTGTAAACACAGGGGACACAGCTTAACAATGCAACTTTTAATTACTGTTTTCTTTTTTCTTAACCTACTAATAGTTTGTTGATCTGATAAGCAAGAGTGGGCGGGTGAGAAAAACCGAATTGGGTTTAGTCAATCACTGCACTGCATGCAAACAAGAAACGTGTCACACTTGTGACGTCGGGCATTCATATAGGAAGAACGCGGTGTGTAACACTGTGTACACCTCAAATACCACCCCAACCCACTCCCTGTAGTGAATCCTCTGTTTAGAACACCAAAGATAAGGACTAGATACTACTTTCTCTTTTTCGTATAATCTTGTAGACACTTACTTGATGATTTTTAACTTTTTATTTCTAAATGAGACGAAATGCTGATGTATCCTTTCATTCAGCTAACAAACTAGAAAAGGTTATGTTCATTTTTCAAAAAGGGAAGTAAGCAAACAAATATTGCCAACTCTTCTATTTATGGATATCACACATATCAGCAGGAGTAATAAATTTACTCACAGCACTTGTTTTCAGGACAACACTTCATTTTCAGGAAATCTACTTCCTACAGAGCCAAAATGCCATTTAGCAATAAATAACACTTGTCAGCCTCAGAGCATTTAAGGAAACTAGACAAGTAAAATTATCCTCTTTGTAATTTAATGAAAAGGTACAACAGAATAATGCATGATGAACTCACCTAATTATGAGGTGGGAGGAGCGAAATCTAAATTTCTTTTGCTATAGTTATACATCAATTTAAAAAGCAAAAAAAAAAAAGGGGGGGGCAATCTCTCTCTGTGTCTTTCTCTCTCTCTCTTCCTCTCCCTCTCTCTTTTCATTGTGTATCAGTTTCCATGAAAGACCTGAATACCACTTACCTCAAATTAAGCATATGTGTTACTTCAAGTAATACGTTTTGACATAAGATGGTTGACCAAGGTGCTTTTCTTCGGCTTGAGTTCACCATCTCTTCATTCAAACTGCACTTTTAGCCAGAGATGCAATATATCCCCACTACTCAATACTACCTCTGAATGTTACAACGAATTTACAGTCTAGTACTTATTACATGCTGCTATACACAAGCAATGCAAGAAAAAAACTTACTGGGTAGGTGATTCTAATCATCTGCAGTTCTTTTTGTACACTTAATTACAGTTAAAGAAGCAATCTCCTTACTGTGTTTCAGCATGACTATGTATTTTTCTATGTTTTTTTAATTAAAAATTTTTAAAATACTTGTTTCAGCTTCTCTGCTAGATTTCTACATTAACTTGAAAATTTTTTAACCAAGTCGCTCCTAGGTTCTTAAGGATAATTTTCCTCAATCACACTACACATCACACAAGATTTGACTGTAATATTTAAATATTACCCTCCAAGTCTGTACCTCAAATGAATTCTTTAAGGAGATGGACTAATTGACTTGCAAAGACCTACCTCCAGACTTCAAAAGGAATGAACTTGTTACTTGCAGCATTCATTTGTTTTTTCAATGTTTGAAATAGTTCAAACTGCAGCTAACCCTAGTCAAAACTATTTTTGTAAAAGACATTTGATAGAAAGGAACACGTTTTTACATACTTTTGCAAAATAAGTAAATAATAAATAAAATAAAAGCCAACCTTCAAAGAAACTTGAAGCTTTGTAGGTGAGATGCAACAAGCCCTGCTTTTGCATAATGCAATCAAAAATATGTGTTTTTAAGATTAGTTGAATATAAGAAAATGCTTGACAAATATTTTCATGTATTTTACACAAATGTGATTTTTGTAATATGTCTCAACCAGATTTATTTTAAACGCTTCTTATGTAGAGTTTTTATGCCTTTCTCTCCTAGTGAGTGTGCTGACTTTTTAACATGGTATTATCAACTGGGCCAGGAGGTAGTTTCTCATGACGGCTTTTGTCAGTATGGCTTTTAGTACTGAAGCCAAATGAAACTCAAAACCATCTCTCTTCCAGCTGCTTCAGGGAGGTAGTTTCAAAGGCCACATACCTCTCTGAGACTGGCAGATCGCTCACTGTTGTGAATCACCAAAGGAGCTATGGAGAGAATTAAAACTCAACATTACTGTTAACTGTGCGTTAAATAAGCAAATAAACAGTGGCTCATAAAAATAAAAGTCGCATTCCATATCTTTGGATGGGCCTTTTAGAAACCTCATTGGCCAGCTCATAAAATGGAAGCAATTGCTCATGTTGGCCAAACATGGTGCACCGAGTGATTTCCATCTCTGGTAAAGTTACACTTTTATTTCCTGTATGTTGTACAATCAAAACACACTACTACCTCTTAAGTCCCAGTATACCTCATTTTTCATACTGAAAAAAAAAGCTTGTGGCCAATGGAACAGTAAGAACATCATAAAATTTTTATATATATAGTTTATTTTTGTGGGAGATAAATTTTATAGGACTGTTCTTTGCTGTTGTTGGTCGCAGCTACATAAGACTGGACATTTAACTTTTCTACCATTTCTGCAAGTTAGGTATGTTTGCAGGAGAAAAGTATCAAGACGTTTAACTGCAGTTGACTTTCTCCCTGTTCCTTTGAGTGTCTTCTAACTTTATTCTTTGTTCTTTATGTAGAATTGCTGTCTATGATTGTACTTTGAATCGCTTGCTTGTTGAAAATATTTCTCTAGTGTATTATCACTGTCTGTTCTGCACAATAAACATAACAGCCTCTGTGATCCCCATGTGTTTTGATTCCTGCTCTTTGTTACAGTTCCATTAAATGAGTAATAAAGTTTGGTCAAAACAGATCAA hmga2_human High mobility group protein HMGI-C High mobility group AT-hook protein 2 HMGA2 HMGIC protein protein human Homo sapiens Human MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWPQQVVQKKPAQEETEETSSQESAEED F36BABE623DA4615 hsa-mir-367-3p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA human Homo sapiens Human AAUUGCACUUUAGCAAUGGUGA hsa-mir-302a-5p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA human Homo sapiens Human ACUUAAACGUGGAUGUACUUGCU runx1_human Runt-related transcription factor 1 RUNX1 AML1 CBFA2 Core-binding factor subunit alpha-2 Acute myeloid leukemia 1 protein Oncogene AML-1 Polyomavirus enhancer-binding protein 2 alpha B subunit SL3-3 enhancer factor 1 alpha B subunit SL3/AKV core-binding factor alpha B subunit protein protein human Homo sapiens Human MRIPVDASTSRRFTPPSTALSPGKMSEALPLGAPDAGAALAGKLRSGDRSMVEVLADHPGELVRTDSPNFLCSVLPTHWRCNKTLPIAFKVVALGDVPDGTLVTVMAGNDENYSAELRNATAAMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPPQVATYHRAIKITVDGPREPRRHRQKLDDQTKPGSLSFSERLSELEQLRRTAMRVSPHHPAPTPNPRASLNHSTAFNPQPQSQMQDTRQIQPSPPWSYDQSYQYLGSIASPSVHPATPISPGRASGMTTLSAELSSRLSTAPDLTAFSDPRQFPALPSISDPRMHYPGAFTYSPTPVTSGIGIGMSAMGSATRYHTYLPPPYPGSSQAQGGPFQASSPSYHLYYGASAGSYQFSMVGGERSPPRILPPCTNASTGSALLNPSLPNQSDVVEAEGSHSNSPTNMAPSARLEEAVWRPY DIP protein Q01196 original sequence version: 171 DIP protein Q01196 original sequence version: 189 DIP protein Q01196 original sequence version: 132 4F1F193A7CADDBAB hsa_mir_302a_5p-mrna_hmga2-1 2 4 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered tag luciferase tag luciferase tag 3' range 3' range 3' range 3' range r.3404_3410delinsaaattct mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain TTTAAGA AAATTCT 7 predetermined predetermined participant predetermined tag luciferase tag visualisation by luciferase assay tag luciferase regulator regulator modulator regulator neutral component neutral component engineered engineered purified purified physical association physical association FIG 3G sequences of miR 302a and 367 have been inverted in Figure 3 down-regulates quantity by destabilization hsa_mir_367_3p-mrna_hmga2-1 2 6 predetermined predetermined participant predetermined tag luciferase tag visualisation by luciferase assay tag luciferase regulator regulator modulator regulator neutral component neutral component engineered engineered purified purified 4 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered r.2701_2707delinsacgttat mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain TGCAATA ACGTTAT tag luciferase tag luciferase tag 3' range 3' range 3' range 3' range physical association physical association FIG 3G sequences of miR 302a and 367 have been inverted in Figure 3 down-regulates quantity by destabilization hmga2-runx1-1 3 8 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 5 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Suppl Fig S4h hmga2-runx1-2 1 8 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 5 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Suppl Fig S4h