MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Staphylococcus aureus SdrE captures complement factor H's C-terminus via a novel 'close, dock, lock and latch' mechanism for complement evasion. Staphylococcus aureus SdrE captures complement factor H's C-terminus via a novel 'close, dock, lock and latch' mechanism for complement evasion. The Biochemical journal 2017 imex curation Zhang Y., Wu M., Hang T., Wang C., Yang Y., Pan W., Zang J., Zhang M., Zhang X., Zhang Y., Wu M., Hang T., Wang C., Yang Y., Pan W., Zang J., Zhang M., Zhang X. xuanzbin@ustc.edu.cn; zhmin07@ustc.edu 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Zhang Y., Wu M., Hang T., Wang C., Yang Y., Pan W., Zang J., Zhang M., Zhang X., Zhang Y., Wu M., Hang T., Wang C., Yang Y., Pan W., Zang J., Zhang M., Zhang X. xuanzbin@ustc.edu.cn; zhmin07@ustc.edu Accepted 2020-DEC-03 AT 13:07 GMT AT 13:07 GMT by MARTA The Biochemical journal 2017 Only protein-protein interactions imex curation imex curation Staphylococcus aureus SdrE captures complement factor H's C-terminus via a novel 'close, dock, lock and latch' mechanism for complement evasion. Staphylococcus aureus SdrE captures complement factor H's C-terminus via a novel 'close, dock, lock and latch' mechanism for complement evasion. The Biochemical journal 2017 imex curation Zhang Y., Wu M., Hang T., Wang C., Yang Y., Pan W., Zang J., Zhang M., Zhang X., Zhang Y., Wu M., Hang T., Wang C., Yang Y., Pan W., Zang J., Zhang M., Zhang X. xuanzbin@ustc.edu.cn; zhmin07@ustc.edu 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Zhang Y., Wu M., Hang T., Wang C., Yang Y., Pan W., Zang J., Zhang M., Zhang X., Zhang Y., Wu M., Hang T., Wang C., Yang Y., Pan W., Zang J., Zhang M., Zhang X. xuanzbin@ustc.edu.cn; zhmin07@ustc.edu Accepted 2020-DEC-03 AT 13:07 GMT AT 13:07 GMT by MARTA The Biochemical journal 2017 Only protein-protein interactions imex curation imex curation Staphylococcus aureus SdrE captures complement factor H's C-terminus via a novel 'close, dock, lock and latch' mechanism for complement evasion. Staphylococcus aureus SdrE captures complement factor H's C-terminus via a novel 'close, dock, lock and latch' mechanism for complement evasion. The Biochemical journal 2017 imex curation Zhang Y., Wu M., Hang T., Wang C., Yang Y., Pan W., Zang J., Zhang M., Zhang X., Zhang Y., Wu M., Hang T., Wang C., Yang Y., Pan W., Zang J., Zhang M., Zhang X. xuanzbin@ustc.edu.cn; zhmin07@ustc.edu 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Zhang Y., Wu M., Hang T., Wang C., Yang Y., Pan W., Zang J., Zhang M., Zhang X., Zhang Y., Wu M., Hang T., Wang C., Yang Y., Pan W., Zang J., Zhang M., Zhang X. xuanzbin@ustc.edu.cn; zhmin07@ustc.edu Accepted 2020-DEC-03 AT 13:07 GMT AT 13:07 GMT by MARTA The Biochemical journal 2017 Only protein-protein interactions imex curation imex curation Staphylococcus aureus SdrE captures complement factor H's C-terminus via a novel 'close, dock, lock and latch' mechanism for complement evasion. Staphylococcus aureus SdrE captures complement factor H's C-terminus via a novel 'close, dock, lock and latch' mechanism for complement evasion. The Biochemical journal 2017 imex curation Zhang Y., Wu M., Hang T., Wang C., Yang Y., Pan W., Zang J., Zhang M., Zhang X., Zhang Y., Wu M., Hang T., Wang C., Yang Y., Pan W., Zang J., Zhang M., Zhang X. xuanzbin@ustc.edu.cn; zhmin07@ustc.edu 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down predetermined predetermined participant predetermined Zhang Y., Wu M., Hang T., Wang C., Yang Y., Pan W., Zang J., Zhang M., Zhang X., Zhang Y., Wu M., Hang T., Wang C., Yang Y., Pan W., Zang J., Zhang M., Zhang X. xuanzbin@ustc.edu.cn; zhmin07@ustc.edu Accepted 2020-DEC-03 AT 13:07 GMT AT 13:07 GMT by MARTA The Biochemical journal 2017 Only protein-protein interactions imex curation imex curation cfah_human Complement factor H CFH HF HF1 HF2 H factor 1 protein protein human Homo sapiens Human MRLLAKIICLMLWAICVAEDCNELPPRRNTEILTGSWSDQTYPEGTQAIYKCRPGYRSLGNVIMVCRKGEWVALNPLRKCQKRPCGHPGDTPFGTFTLTGGNVFEYGVKAVYTCNEGYQLLGEINYRECDTDGWTNDIPICEVVKCLPVTAPENGKIVSSAMEPDREYHFGQAVRFVCNSGYKIEGDEEMHCSDDGFWSKEKPKCVEISCKSPDVINGSPISQKIIYKENERFQYKCNMGYEYSERGDAVCTESGWRPLPSCEEKSCDNPYIPNGDYSPLRIKHRTGDEITYQCRNGFYPATRGNTAKCTSTGWIPAPRCTLKPCDYPDIKHGGLYHENMRRPYFPVAVGKYYSYYCDEHFETPSGSYWDHIHCTQDGWSPAVPCLRKCYFPYLENGYNQNYGRKFVQGKSIDVACHPGYALPKAQTTVTCMENGWSPTPRCIRVKTCSKSSIDIENGFISESQYTYALKEKAKYQCKLGYVTADGETSGSITCGKDGWSAQPTCIKSCDIPVFMNARTKNDFTWFKLNDTLDYECHDGYESNTGSTTGSIVCGYNGWSDLPICYERECELPKIDVHLVPDRKKDQYKVGEVLKFSCKPGFTIVGPNSVQCYHFGLSPDLPICKEQVQSCGPPPELLNGNVKEKTKEEYGHSEVVEYYCNPRFLMKGPNKIQCVDGEWTTLPVCIVEESTCGDIPELEHGWAQLSSPPYYYGDSVEFNCSESFTMIGHRSITCIHGVWTQLPQCVAIDKLKKCKSSNLIILEEHLKNKKEFDHNSNIRYRCRGKEGWIHTVCINGRWDPEVNCSMAQIQLCPPPPQIPNSHNMTTTLNYRDGEKVSVLCQENYLIQEGEEITCKDGRWQSIPLCVEKIPCSQPPQIEHGTINSSRSSQESYAHGTKLSYTCEGGFRISEENETTCYMGKWSSPPQCEGLPCKSPPEISHGVVAHMSDSYQYGEEVTYKCFEGFGIDGPAIAKCLGEKWSHPPSCIKTDCLSLPSFENAIPMGEKKDVYKAGEQVTYTCATYYKMDGASNVTCINSRWTGRPTCRDTSCVNPPTVQNAYIVSRQMSKYPSGERVRYQCRSPYEMFGDEEVMCLNGNWTEPPQCKDSTGKCGPPPPIDNGDITSFPLSVYAPASSVEYQCQNLYQLEGNKRITCRNGQWSEPPKCLHPCVISREIMENYNIALRWTAKQKLYSRTGESVEFVCKRGYRLSSRSHTLRTTCWDGKLEYPTCAKR DIP protein P08603 original sequence version: 161 DIP protein P08603-1 original sequence version: 149 3C26D62A2BF9BFEE sdre_staam Serine-aspartate repeat-containing protein E sdrE SAV0563 protein protein staam Staphylococcus aureus (strain Mu50 / ATCC 700699) MINRDNKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFGLGNQEAKAAENTSTENAKQDDATTSDNKEVVSETENNSTTENDSTNPIKKETNTDSQPEAKEESTTSSTQQQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNYTNNDVTTKPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPVNVSKEELKNNPEKLKELVRNDNNTDRSTKPVATAPTSVAPKRLNAKMRFAVAQPAAVASNNVNDLITVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKNVIPSDLTDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKARLTLYSYIDKQAVPNETSLNLTFATAGKETSQNVSVDYQDPMVHGDSNIQSIFTKLDENKQTIEQQIYVNPLKKTATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNPNQQLPQSNRIYDFSQYEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQGTSMRTTDKYGYYNYAGYSNFIVTSNDTGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQGTDSKEKPMANVLVTLTYPDGTTKSVRTDANGHYEFGGLKDGETYTVKFETPAGYLPTKVNGTTDGEKDSNGSSITVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDANEPGIKDVKVTLKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTTAEDKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKDVKVTLLNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTVTNTTEDDKDADGGEVDVTITDHDDFILDNGYFEEDTSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAGKHTPVKPMSTTKDHHNKAKALPETGSENNGSNNATLFGGLFAALGSLLLFGRRKKQNK E679F7C2991846D9 cfh-sdre-1 1 5 unspecified role unspecified role neutral component neutral component purified purified binding range binding range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range binding range binding range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction F3A F3D T1 SF2B cfh-sdre-2 4 5 unspecified role unspecified role neutral component neutral component purified purified P08603:p.Ser1208Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain S A Thu Oct 29 21:37:42 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P08603:p.[Ser1208Ala]' P08603:p.Arg1210Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain R A Thu Oct 29 22:41:18 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P08603:p.[Arg1210Ala]' P08603:p.Ser1211Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain S A Thu Oct 29 22:22:58 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P08603:p.[Ser1211Ala]' P08603:p.His1212Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain H A Thu Oct 29 22:10:20 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P08603:p.[His1212Ala]' P08603:p.Thr1213Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain T A Thu Oct 29 21:51:00 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P08603:p.[Thr1213Ala]' P08603:p.Leu1214Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain L A Thu Oct 29 22:15:32 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P08603:p.[Leu1214Ala]' P08603:p.Arg1215Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R A Thu Oct 29 22:54:10 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P08603:p.[Arg1215Ala]' P08603:p.Thr1216Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain T A Thu Oct 29 22:06:56 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P08603:p.[Thr1216Ala]' P08603:p.Thr1217Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain T A Thu Oct 29 21:23:56 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P08603:p.[Thr1217Ala]' P08603:p.Arg1206Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain R A Thu Oct 29 21:48:45 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P08603:p.[Arg1206Ala]' P08603:p.Leu1207Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain L A Thu Oct 29 22:21:46 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P08603:p.[Leu1207Ala]' P08603:p.Cys1218Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain C A Thu Oct 29 22:28:34 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P08603:p.[Leu1223Ala]' P08603:p.Trp1219Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain W A Thu Oct 29 22:33:36 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P08603:p.[Trp1219Ala]' P08603:p.Asp1220Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain D A Thu Oct 29 22:36:53 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P08603:p.[Asp1220Ala]' P08603:p.Lys1222Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Thu Oct 29 21:52:43 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P08603:p.[Lys1222Ala]' P08603:p.Leu1223Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain L A Thu Oct 29 22:28:34 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P08603:p.[Leu1223Ala]' P08603:p.Glu1224Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain E A Thu Oct 29 22:55:25 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P08603:p.[Glu1224Ala]' P08603:p.Tyr1225Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain Y A Thu Oct 29 22:29:06 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P08603:p.[Tyr1225Ala]' P08603:p.Pro1226Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain P A Thu Oct 29 21:45:34 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P08603:p.[Pro1226Ala]' P08603:p.Ser1209Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain S A Thu Oct 29 22:49:14 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P08603:p.[Ser1209Ala]' binding range binding range sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST tag GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component purified purified Q932F7:p.Asp334Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain D A Thu Oct 29 22:34:21 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'Q932F7:p.[Asp334Ala]' Q932F7:p.Leu335Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain L A Thu Oct 29 22:40:48 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'Q932F7:p.[Leu335Ala]' Q932F7:p.Gln419Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain Q A Thu Oct 29 21:48:03 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'Q932F7:p.[Gln419Ala]' Q932F7:p.Asp588Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain D A Thu Oct 29 22:58:22 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'Q932F7:p.[Leu588Ala]' binding range binding range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction F1B F1C F1D F3B F3C cfh-sdre-3 2 5 unspecified role unspecified role neutral component neutral component purified purified binding range binding range sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST tag GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component purified purified binding range binding range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction F1E cfh-sdre-4 3 5 unspecified role unspecified role neutral component neutral component purified purified binding range binding range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component purified purified binding range binding range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction SF2B